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<span>Applications</span> </div> </a> <button aria-controls="toc-Applications-sublist" class="cdx-button cdx-button--weight-quiet cdx-button--icon-only vector-toc-toggle"> <span class="vector-icon mw-ui-icon-wikimedia-expand"></span> <span>Toggle Applications subsection</span> </button> <ul id="toc-Applications-sublist" class="vector-toc-list"> <li id="toc-Precision_cut_kidney_slices" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#Precision_cut_kidney_slices"> <div class="vector-toc-text"> <span class="vector-toc-numb">2.1</span> <span>Precision cut kidney slices</span> </div> </a> <ul id="toc-Precision_cut_kidney_slices-sublist" class="vector-toc-list"> </ul> </li> </ul> </li> <li id="toc-Types" class="vector-toc-list-item vector-toc-level-1 vector-toc-list-item-expanded"> <a class="vector-toc-link" href="#Types"> <div class="vector-toc-text"> <span class="vector-toc-numb">3</span> <span>Types</span> </div> </a> <button aria-controls="toc-Types-sublist" class="cdx-button cdx-button--weight-quiet cdx-button--icon-only vector-toc-toggle"> <span class="vector-icon mw-ui-icon-wikimedia-expand"></span> <span>Toggle Types subsection</span> </button> <ul id="toc-Types-sublist" class="vector-toc-list"> <li id="toc-Sledge" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#Sledge"> <div class="vector-toc-text"> <span class="vector-toc-numb">3.1</span> <span>Sledge</span> </div> </a> <ul id="toc-Sledge-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-Rotary" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#Rotary"> <div class="vector-toc-text"> <span class="vector-toc-numb">3.2</span> <span>Rotary</span> </div> </a> <ul id="toc-Rotary-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-Cryomicrotome" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#Cryomicrotome"> <div class="vector-toc-text"> <span class="vector-toc-numb">3.3</span> <span>Cryomicrotome</span> </div> </a> <ul id="toc-Cryomicrotome-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-Ultramicrotome" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#Ultramicrotome"> <div class="vector-toc-text"> <span class="vector-toc-numb">3.4</span> <span>Ultramicrotome</span> </div> </a> <ul id="toc-Ultramicrotome-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-Vibrating" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#Vibrating"> <div class="vector-toc-text"> <span class="vector-toc-numb">3.5</span> <span>Vibrating</span> </div> </a> <ul id="toc-Vibrating-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-Saw" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#Saw"> <div class="vector-toc-text"> <span class="vector-toc-numb">3.6</span> <span>Saw</span> </div> </a> <ul id="toc-Saw-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-Laser" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#Laser"> <div class="vector-toc-text"> <span class="vector-toc-numb">3.7</span> <span>Laser</span> </div> </a> <ul id="toc-Laser-sublist" class="vector-toc-list"> </ul> </li> </ul> </li> <li id="toc-Knives" class="vector-toc-list-item vector-toc-level-1 vector-toc-list-item-expanded"> <a class="vector-toc-link" href="#Knives"> <div class="vector-toc-text"> <span class="vector-toc-numb">4</span> <span>Knives</span> </div> </a> <button aria-controls="toc-Knives-sublist" class="cdx-button cdx-button--weight-quiet cdx-button--icon-only vector-toc-toggle"> <span class="vector-icon mw-ui-icon-wikimedia-expand"></span> <span>Toggle Knives subsection</span> </button> <ul id="toc-Knives-sublist" class="vector-toc-list"> <li id="toc-Design_and_cut_types" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#Design_and_cut_types"> <div class="vector-toc-text"> <span class="vector-toc-numb">4.1</span> <span>Design and cut types</span> </div> </a> <ul id="toc-Design_and_cut_types-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-Sectioning" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#Sectioning"> <div class="vector-toc-text"> <span class="vector-toc-numb">4.2</span> <span>Sectioning</span> </div> </a> <ul id="toc-Sectioning-sublist" class="vector-toc-list"> </ul> </li> </ul> </li> <li id="toc-See_also" class="vector-toc-list-item vector-toc-level-1 vector-toc-list-item-expanded"> <a class="vector-toc-link" href="#See_also"> <div class="vector-toc-text"> <span class="vector-toc-numb">5</span> <span>See also</span> </div> </a> <ul id="toc-See_also-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-References" class="vector-toc-list-item vector-toc-level-1 vector-toc-list-item-expanded"> <a class="vector-toc-link" href="#References"> <div class="vector-toc-text"> <span class="vector-toc-numb">6</span> <span>References</span> </div> </a> <ul id="toc-References-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-External_links" class="vector-toc-list-item vector-toc-level-1 vector-toc-list-item-expanded"> <a class="vector-toc-link" href="#External_links"> <div class="vector-toc-text"> <span class="vector-toc-numb">7</span> <span>External links</span> </div> </a> <ul id="toc-External_links-sublist" class="vector-toc-list"> </ul> </li> </ul> </div> </div> </nav> </div> </div> <div class="mw-content-container"> <main id="content" class="mw-body"> <header class="mw-body-header vector-page-titlebar"> <nav aria-label="Contents" class="vector-toc-landmark"> <div id="vector-page-titlebar-toc" class="vector-dropdown vector-page-titlebar-toc vector-button-flush-left" > <input type="checkbox" id="vector-page-titlebar-toc-checkbox" role="button" aria-haspopup="true" data-event-name="ui.dropdown-vector-page-titlebar-toc" class="vector-dropdown-checkbox 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Available in 28 languages" > <label id="p-lang-btn-label" for="p-lang-btn-checkbox" class="vector-dropdown-label cdx-button cdx-button--fake-button cdx-button--fake-button--enabled cdx-button--weight-quiet cdx-button--action-progressive mw-portlet-lang-heading-28" aria-hidden="true" ><span class="vector-icon mw-ui-icon-language-progressive mw-ui-icon-wikimedia-language-progressive"></span> <span class="vector-dropdown-label-text">28 languages</span> </label> <div class="vector-dropdown-content"> <div class="vector-menu-content"> <ul class="vector-menu-content-list"> <li class="interlanguage-link interwiki-ar mw-list-item"><a href="https://ar.wikipedia.org/wiki/%D9%85%D8%B4%D8%B1%D8%A7%D8%AD" title="مشراح – Arabic" lang="ar" hreflang="ar" data-title="مشراح" data-language-autonym="العربية" data-language-local-name="Arabic" class="interlanguage-link-target"><span>العربية</span></a></li><li class="interlanguage-link interwiki-bg mw-list-item"><a href="https://bg.wikipedia.org/wiki/%D0%9C%D0%B8%D0%BA%D1%80%D0%BE%D1%82%D0%BE%D0%BC" title="Микротом – Bulgarian" lang="bg" hreflang="bg" data-title="Микротом" data-language-autonym="Български" data-language-local-name="Bulgarian" class="interlanguage-link-target"><span>Български</span></a></li><li class="interlanguage-link interwiki-ca mw-list-item"><a href="https://ca.wikipedia.org/wiki/Micr%C3%B2tom" title="Micròtom – Catalan" lang="ca" hreflang="ca" data-title="Micròtom" data-language-autonym="Català" data-language-local-name="Catalan" class="interlanguage-link-target"><span>Català</span></a></li><li class="interlanguage-link interwiki-cs mw-list-item"><a href="https://cs.wikipedia.org/wiki/Mikrotom" title="Mikrotom – Czech" lang="cs" hreflang="cs" data-title="Mikrotom" data-language-autonym="Čeština" data-language-local-name="Czech" class="interlanguage-link-target"><span>Čeština</span></a></li><li class="interlanguage-link interwiki-da mw-list-item"><a href="https://da.wikipedia.org/wiki/Mikrotom" title="Mikrotom – Danish" lang="da" hreflang="da" data-title="Mikrotom" data-language-autonym="Dansk" data-language-local-name="Danish" class="interlanguage-link-target"><span>Dansk</span></a></li><li class="interlanguage-link interwiki-de badge-Q17437796 badge-featuredarticle mw-list-item" title="featured article badge"><a href="https://de.wikipedia.org/wiki/Mikrotom" title="Mikrotom – German" lang="de" hreflang="de" data-title="Mikrotom" data-language-autonym="Deutsch" data-language-local-name="German" class="interlanguage-link-target"><span>Deutsch</span></a></li><li class="interlanguage-link interwiki-et mw-list-item"><a href="https://et.wikipedia.org/wiki/Mikrotoom" title="Mikrotoom – Estonian" lang="et" hreflang="et" data-title="Mikrotoom" data-language-autonym="Eesti" data-language-local-name="Estonian" class="interlanguage-link-target"><span>Eesti</span></a></li><li class="interlanguage-link interwiki-el mw-list-item"><a href="https://el.wikipedia.org/wiki/%CE%9C%CE%B9%CE%BA%CF%81%CE%BF%CF%84%CF%8C%CE%BC%CE%BF%CF%82" title="Μικροτόμος – Greek" lang="el" hreflang="el" data-title="Μικροτόμος" data-language-autonym="Ελληνικά" data-language-local-name="Greek" class="interlanguage-link-target"><span>Ελληνικά</span></a></li><li class="interlanguage-link interwiki-es mw-list-item"><a href="https://es.wikipedia.org/wiki/Microtomo" title="Microtomo – Spanish" lang="es" hreflang="es" data-title="Microtomo" data-language-autonym="Español" data-language-local-name="Spanish" class="interlanguage-link-target"><span>Español</span></a></li><li class="interlanguage-link interwiki-eo mw-list-item"><a href="https://eo.wikipedia.org/wiki/Mikrotomo" title="Mikrotomo – Esperanto" lang="eo" hreflang="eo" data-title="Mikrotomo" data-language-autonym="Esperanto" data-language-local-name="Esperanto" class="interlanguage-link-target"><span>Esperanto</span></a></li><li class="interlanguage-link interwiki-eu mw-list-item"><a href="https://eu.wikipedia.org/wiki/Mikrotomo" title="Mikrotomo – Basque" lang="eu" hreflang="eu" data-title="Mikrotomo" data-language-autonym="Euskara" data-language-local-name="Basque" class="interlanguage-link-target"><span>Euskara</span></a></li><li class="interlanguage-link interwiki-fa mw-list-item"><a href="https://fa.wikipedia.org/wiki/%D9%85%DB%8C%DA%A9%D8%B1%D9%88%D8%AA%D9%88%D9%85" title="میکروتوم – Persian" lang="fa" hreflang="fa" data-title="میکروتوم" data-language-autonym="فارسی" data-language-local-name="Persian" class="interlanguage-link-target"><span>فارسی</span></a></li><li class="interlanguage-link interwiki-fr mw-list-item"><a href="https://fr.wikipedia.org/wiki/Microtome" title="Microtome – French" lang="fr" hreflang="fr" data-title="Microtome" data-language-autonym="Français" data-language-local-name="French" class="interlanguage-link-target"><span>Français</span></a></li><li class="interlanguage-link interwiki-gl mw-list-item"><a href="https://gl.wikipedia.org/wiki/Micr%C3%B3tomo" title="Micrótomo – Galician" lang="gl" hreflang="gl" data-title="Micrótomo" data-language-autonym="Galego" data-language-local-name="Galician" class="interlanguage-link-target"><span>Galego</span></a></li><li class="interlanguage-link interwiki-ko mw-list-item"><a href="https://ko.wikipedia.org/wiki/%EB%A7%88%EC%9D%B4%ED%81%AC%EB%A1%9C%ED%86%B0" title="마이크로톰 – Korean" lang="ko" hreflang="ko" data-title="마이크로톰" data-language-autonym="한국어" data-language-local-name="Korean" class="interlanguage-link-target"><span>한국어</span></a></li><li class="interlanguage-link interwiki-id mw-list-item"><a href="https://id.wikipedia.org/wiki/Mikrotom" title="Mikrotom – Indonesian" lang="id" hreflang="id" data-title="Mikrotom" data-language-autonym="Bahasa Indonesia" data-language-local-name="Indonesian" class="interlanguage-link-target"><span>Bahasa Indonesia</span></a></li><li class="interlanguage-link interwiki-it mw-list-item"><a href="https://it.wikipedia.org/wiki/Microtomo" title="Microtomo – Italian" lang="it" hreflang="it" data-title="Microtomo" data-language-autonym="Italiano" data-language-local-name="Italian" class="interlanguage-link-target"><span>Italiano</span></a></li><li class="interlanguage-link interwiki-he mw-list-item"><a href="https://he.wikipedia.org/wiki/%D7%9E%D7%99%D7%A7%D7%A8%D7%95%D7%98%D7%95%D7%9D" title="מיקרוטום – Hebrew" lang="he" hreflang="he" data-title="מיקרוטום" data-language-autonym="עברית" data-language-local-name="Hebrew" class="interlanguage-link-target"><span>עברית</span></a></li><li class="interlanguage-link interwiki-ms mw-list-item"><a href="https://ms.wikipedia.org/wiki/Mikrotom" title="Mikrotom – Malay" lang="ms" hreflang="ms" data-title="Mikrotom" data-language-autonym="Bahasa Melayu" data-language-local-name="Malay" class="interlanguage-link-target"><span>Bahasa Melayu</span></a></li><li class="interlanguage-link interwiki-nl mw-list-item"><a href="https://nl.wikipedia.org/wiki/Microtoom" title="Microtoom – Dutch" lang="nl" hreflang="nl" data-title="Microtoom" data-language-autonym="Nederlands" data-language-local-name="Dutch" class="interlanguage-link-target"><span>Nederlands</span></a></li><li class="interlanguage-link interwiki-ja mw-list-item"><a href="https://ja.wikipedia.org/wiki/%E3%83%9F%E3%82%AF%E3%83%AD%E3%83%88%E3%83%BC%E3%83%A0" title="ミクロトーム – Japanese" lang="ja" hreflang="ja" data-title="ミクロトーム" data-language-autonym="日本語" data-language-local-name="Japanese" class="interlanguage-link-target"><span>日本語</span></a></li><li class="interlanguage-link interwiki-pl mw-list-item"><a href="https://pl.wikipedia.org/wiki/Mikrotom" title="Mikrotom – Polish" lang="pl" hreflang="pl" data-title="Mikrotom" data-language-autonym="Polski" data-language-local-name="Polish" class="interlanguage-link-target"><span>Polski</span></a></li><li class="interlanguage-link interwiki-pt mw-list-item"><a href="https://pt.wikipedia.org/wiki/Micr%C3%B3tomo" title="Micrótomo – Portuguese" lang="pt" hreflang="pt" data-title="Micrótomo" data-language-autonym="Português" data-language-local-name="Portuguese" class="interlanguage-link-target"><span>Português</span></a></li><li class="interlanguage-link interwiki-ru mw-list-item"><a href="https://ru.wikipedia.org/wiki/%D0%9C%D0%B8%D0%BA%D1%80%D0%BE%D1%82%D0%BE%D0%BC" title="Микротом – Russian" lang="ru" hreflang="ru" data-title="Микротом" data-language-autonym="Русский" data-language-local-name="Russian" class="interlanguage-link-target"><span>Русский</span></a></li><li class="interlanguage-link interwiki-fi mw-list-item"><a href="https://fi.wikipedia.org/wiki/Mikrotomi" title="Mikrotomi – Finnish" lang="fi" hreflang="fi" data-title="Mikrotomi" data-language-autonym="Suomi" data-language-local-name="Finnish" class="interlanguage-link-target"><span>Suomi</span></a></li><li class="interlanguage-link interwiki-sv mw-list-item"><a href="https://sv.wikipedia.org/wiki/Mikrotom" title="Mikrotom – Swedish" lang="sv" hreflang="sv" data-title="Mikrotom" data-language-autonym="Svenska" data-language-local-name="Swedish" class="interlanguage-link-target"><span>Svenska</span></a></li><li class="interlanguage-link interwiki-tr mw-list-item"><a href="https://tr.wikipedia.org/wiki/Mikrotom" title="Mikrotom – Turkish" lang="tr" hreflang="tr" data-title="Mikrotom" data-language-autonym="Türkçe" data-language-local-name="Turkish" class="interlanguage-link-target"><span>Türkçe</span></a></li><li class="interlanguage-link interwiki-uk mw-list-item"><a href="https://uk.wikipedia.org/wiki/%D0%9C%D1%96%D0%BA%D1%80%D0%BE%D1%82%D0%BE%D0%BC" title="Мікротом – Ukrainian" lang="uk" hreflang="uk" data-title="Мікротом" data-language-autonym="Українська" data-language-local-name="Ukrainian" class="interlanguage-link-target"><span>Українська</span></a></li> </ul> <div class="after-portlet after-portlet-lang"><span class="wb-langlinks-edit 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searchaux" style="display:none">Tool to cut fine samples for microscopy</div> <style data-mw-deduplicate="TemplateStyles:r1236090951">.mw-parser-output .hatnote{font-style:italic}.mw-parser-output div.hatnote{padding-left:1.6em;margin-bottom:0.5em}.mw-parser-output .hatnote i{font-style:normal}.mw-parser-output .hatnote+link+.hatnote{margin-top:-0.5em}@media print{body.ns-0 .mw-parser-output .hatnote{display:none!important}}</style><div role="note" class="hatnote navigation-not-searchable">For the moth genus, see <a href="/wiki/Microtome_(moth)" title="Microtome (moth)">Microtome (moth)</a>.</div> <p class="mw-empty-elt"> </p><p>A <b>microtome</b> (from the Greek <i>mikros</i>, meaning "small", and <i>temnein</i>, meaning "to cut") is a <a href="/wiki/Cutting_tool" class="mw-redirect" title="Cutting tool">cutting tool</a> used to produce extremely thin slices of material known as <i>sections</i>, with the process being termed <b>microsectioning</b>. Important in <a href="/wiki/Science" title="Science">science</a>, microtomes are used in <a href="/wiki/Microscopy" title="Microscopy">microscopy</a> for the <a href="/wiki/Microscope_slide" title="Microscope slide">preparation of samples</a> for observation under transmitted <a href="/wiki/Visible_light" class="mw-redirect" title="Visible light">light</a> or <a href="/wiki/Electron" title="Electron">electron</a> radiation. </p><p>Microtomes use <a href="/wiki/Steel" title="Steel">steel</a>, <a href="/wiki/Glass" title="Glass">glass</a> or <a href="/wiki/Diamond" title="Diamond">diamond</a> <a href="/wiki/Blade" title="Blade">blades</a> depending upon the specimen being sliced and the desired thickness of the sections being cut. Steel blades are used to prepare <a href="/wiki/Histological" class="mw-redirect" title="Histological">histological</a> sections of animal or plant tissues for <a href="/wiki/Light_microscopy" class="mw-redirect" title="Light microscopy">light microscopy</a>. Glass knives are used to slice sections for light microscopy and to slice very thin sections for <a href="/wiki/Electron_microscopy" class="mw-redirect" title="Electron microscopy">electron microscopy</a>. Industrial grade diamond knives are used to slice hard materials such as <a href="/wiki/Bone" title="Bone">bone</a>, <a href="/wiki/Teeth" class="mw-redirect" title="Teeth">teeth</a> and tough plant matter for both light microscopy and for electron microscopy. Gem-quality <a href="/wiki/Diamond_knife" title="Diamond knife">diamond knives</a> are also used for slicing thin sections for electron microscopy. </p><p>Microtomy is a method for the preparation of thin sections for materials such as bones, minerals and teeth, and an alternative to <a href="/wiki/Electropolishing" title="Electropolishing">electropolishing</a> and <a href="/wiki/Focused_ion_beam" title="Focused ion beam">ion milling</a>. Microtome sections can be made thin enough to section a human hair across its breadth, with section thickness between 50 <a href="/wiki/Nanometre" title="Nanometre">nm</a> and 100 <a href="/wiki/Micrometre" title="Micrometre">μm</a>. </p> <meta property="mw:PageProp/toc" /> <div class="mw-heading mw-heading2"><h2 id="History">History</h2><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Microtome&action=edit&section=1" title="Edit section: History"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <figure typeof="mw:File/Thumb"><a href="/wiki/File:Cummings_1774_Microtome.jpg" class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/e/e0/Cummings_1774_Microtome.jpg/150px-Cummings_1774_Microtome.jpg" decoding="async" width="150" height="290" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/e/e0/Cummings_1774_Microtome.jpg/225px-Cummings_1774_Microtome.jpg 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/e/e0/Cummings_1774_Microtome.jpg/300px-Cummings_1774_Microtome.jpg 2x" data-file-width="1434" data-file-height="2772" /></a><figcaption>A diagram of a microtome drawn by Cummings in 1770<sup id="cite_ref-Hill_1770_1-0" class="reference"><a href="#cite_note-Hill_1770-1"><span class="cite-bracket">[</span>1<span class="cite-bracket">]</span></a></sup></figcaption></figure> <p>In the beginnings of <a href="/wiki/Light_microscope" class="mw-redirect" title="Light microscope">light microscope</a> development, sections from plants and animals were manually prepared using razor blades. It was found that to observe the structure of the specimen under observation it was important to make clean reproducible cuts on the order of 100 μm, through which light can be transmitted. This allowed for the observation of samples using light microscopes in a transmission mode. </p><p>One of the first devices for the preparation of such cuts was invented in 1770 by George Adams, Jr. (1750–1795) and further developed by <a href="/wiki/Alexander_Cummings" class="mw-redirect" title="Alexander Cummings">Alexander Cummings</a>.<sup id="cite_ref-2" class="reference"><a href="#cite_note-2"><span class="cite-bracket">[</span>2<span class="cite-bracket">]</span></a></sup> The device was hand operated, and the sample held in a cylinder and sections created from the top of the sample using a hand crank.<sup id="cite_ref-Hill_1770_1-1" class="reference"><a href="#cite_note-Hill_1770-1"><span class="cite-bracket">[</span>1<span class="cite-bracket">]</span></a></sup><sup id="cite_ref-Anonymous_1910_3-0" class="reference"><a href="#cite_note-Anonymous_1910-3"><span class="cite-bracket">[</span>3<span class="cite-bracket">]</span></a></sup> </p><p>In 1835, Andrew Prichard developed a table based model which allowed for the vibration to be isolated by affixing the device to the table, separating the operator from the knife.<sup id="cite_ref-Gilbert_Morgan_Smith_4-0" class="reference"><a href="#cite_note-Gilbert_Morgan_Smith-4"><span class="cite-bracket">[</span>4<span class="cite-bracket">]</span></a></sup> </p><p>Occasionally, attribution for the invention of the microtome is given to the anatomist <a href="/wiki/Wilhelm_His,_Sr." class="mw-redirect" title="Wilhelm His, Sr.">Wilhelm His, Sr.</a> (1865).<sup id="cite_ref-5" class="reference"><a href="#cite_note-5"><span class="cite-bracket">[</span>5<span class="cite-bracket">]</span></a></sup><sup id="cite_ref-6" class="reference"><a href="#cite_note-6"><span class="cite-bracket">[</span>6<span class="cite-bracket">]</span></a></sup> In his <span title="German-language text"><i lang="de">Beschreibung eines Mikrotoms</i></span> (German for <i>Description of a Microtome</i>), Wilhelm wrote: </p> <style data-mw-deduplicate="TemplateStyles:r1244412712">.mw-parser-output .templatequote{overflow:hidden;margin:1em 0;padding:0 32px}.mw-parser-output .templatequotecite{line-height:1.5em;text-align:left;margin-top:0}@media(min-width:500px){.mw-parser-output .templatequotecite{padding-left:1.6em}}</style><blockquote class="templatequote"><p>The apparatus has enabled a precision in work by which I can achieve sections that by hand I cannot possibly create. Namely it has enabled the possibility of achieving unbroken sections of objects in the course of research.</p></blockquote> <p>Other sources further attribute the development to a Czech physiologist <a href="/wiki/Jan_Evangelista_Purkyn%C4%9B" title="Jan Evangelista Purkyně">Jan Evangelista Purkyně</a>.<sup id="cite_ref-7" class="reference"><a href="#cite_note-7"><span class="cite-bracket">[</span>7<span class="cite-bracket">]</span></a></sup> Several sources describe the Purkyne model as the first in practical use.<sup id="cite_ref-8" class="reference"><a href="#cite_note-8"><span class="cite-bracket">[</span>8<span class="cite-bracket">]</span></a></sup><sup id="cite_ref-9" class="reference"><a href="#cite_note-9"><span class="cite-bracket">[</span>9<span class="cite-bracket">]</span></a></sup> </p><p>The obscurities in the origins of the microtome are due to the fact that the first microtomes were simply cutting apparatuses, and the developmental phase of early devices is widely undocumented. </p><p>At the end of the 1800s, the development of very thin and consistently thin samples by microtomy, together with the selective staining of important cell components or molecules allowed for the visualisation of microscope details.<sup id="cite_ref-10" class="reference"><a href="#cite_note-10"><span class="cite-bracket">[</span>10<span class="cite-bracket">]</span></a></sup><sup id="cite_ref-11" class="reference"><a href="#cite_note-11"><span class="cite-bracket">[</span>11<span class="cite-bracket">]</span></a></sup> </p><p>Today, the majority of microtomes are a knife-block design with a changeable knife, a specimen holder and an advancement mechanism. In most devices the cutting of the sample begins by moving the sample over the knife, where the advancement mechanism automatically moves forward such that the next cut for a chosen thickness can be made. The section thickness is controlled by an adjustment mechanism, allowing for precise control. </p> <div class="mw-heading mw-heading2"><h2 id="Applications">Applications</h2><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Microtome&action=edit&section=2" title="Edit section: Applications"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <figure class="mw-default-size" typeof="mw:File/Thumb"><a href="/wiki/File:Microtome1905.JPG" class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/9/97/Microtome1905.JPG/220px-Microtome1905.JPG" decoding="async" width="220" height="134" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/9/97/Microtome1905.JPG/330px-Microtome1905.JPG 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/9/97/Microtome1905.JPG/440px-Microtome1905.JPG 2x" data-file-width="3696" data-file-height="2247" /></a><figcaption>Microtome (C. Reichert, Vienna, 1905–1915)</figcaption></figure> <p>The most common applications of <b>microtomes</b> are: </p> <ul><li>Traditional <a href="/wiki/Histological" class="mw-redirect" title="Histological">Histology</a> Technique: tissues are fixed, dehydrated, cleared, and embedded in melted <a href="/wiki/Paraffin_wax" title="Paraffin wax">paraffin</a>, which when cooled forms a solid block. The tissue is then cut in the microtome at thicknesses varying from 2 to 50 μm. From there the tissue can be mounted on a microscope slide, stained with appropriate aqueous dye(s) after removal of the paraffin, and examined using a light microscope.<sup id="cite_ref-Bancroft_and_Stevens,_1982_12-0" class="reference"><a href="#cite_note-Bancroft_and_Stevens,_1982-12"><span class="cite-bracket">[</span>12<span class="cite-bracket">]</span></a></sup></li> <li><a href="/wiki/Frozen_section_procedure" title="Frozen section procedure">Frozen section procedure</a>: water-rich tissues are hardened by freezing and cut in the frozen state with a freezing microtome or microtome-<a href="/wiki/Cryostat" title="Cryostat">cryostat</a>; sections are stained and examined with a light microscope. This technique is much faster than traditional histology (5 minutes vs 16 hours) and is used in conjunction with medical procedures to achieve a quick diagnosis. Cryosections can also be used in <a href="/wiki/Immunohistochemistry" title="Immunohistochemistry">immunohistochemistry</a> as freezing tissue stops degradation of tissue faster than using a <a href="/wiki/Fixation_(histology)" title="Fixation (histology)">fixative</a> and does not alter or mask its chemical composition as much.</li> <li><a href="/wiki/Electron_Microscopy" class="mw-redirect" title="Electron Microscopy">Electron Microscopy</a> Technique: after embedding tissues in epoxy resin, a microtome equipped with a glass or gem grade diamond knife is used to cut very thin sections (typically 60 to 100 nanometer). Sections are stained with an aqueous solution of an appropriate heavy metal salt and examined with a <a href="/wiki/Transmission_electron_microscope" class="mw-redirect" title="Transmission electron microscope">transmission electron microscope</a>. This instrument is often called an <i>ultramicrotome</i>. The ultramicrotome is also used with its glass knife or an industrial grade diamond knife to cut survey sections prior to thin sectioning. These survey sections are generally 0.5 to 1 μm thick and are mounted on a glass slide and stained to locate areas of interest under a light microscope prior to thin sectioning for the TEM. Thin sectioning for the TEM is often done with a gem quality diamond knife. Complementing traditional TEM techniques ultramicrotomes are increasingly found mounted inside an SEM chamber so the surface of the block face can be imaged and then removed with the microtome to uncover the next surface for imaging. This technique is called <a href="/wiki/Serial_block-face_scanning_electron_microscopy" title="Serial block-face scanning electron microscopy">serial block-face scanning electron microscopy</a> (SBFSEM).</li> <li>Botanical Microtomy Technique: hard materials like wood, bone and leather require a <a class="mw-selflink-fragment" href="#Sledge_microtome">sledge microtome</a>. These microtomes have heavier blades and cannot cut as thin as a regular microtome.</li> <li><a href="/wiki/Spectroscopy" title="Spectroscopy">Spectroscopy</a> (especially <a href="/wiki/FTIR" class="mw-redirect" title="FTIR">FTIR</a> or <a href="/wiki/Infrared_spectroscopy" title="Infrared spectroscopy">infrared spectroscopy</a>) Technique: thin polymer sections are needed in order that the infra-red beam can penetrate the sample under examination. It is normal to cut samples to between 20 and 100 μm in thickness. For more detailed analysis of much smaller areas in a thin section, FTIR <a href="/wiki/Microscopy" title="Microscopy">microscopy</a> can be used for sample inspection.</li></ul> <p>A recent development is the <a href="/wiki/Laser_microtome" title="Laser microtome">laser microtome</a>, which cuts the target specimen with a <a href="/wiki/Femtosecond_laser" class="mw-redirect" title="Femtosecond laser">femtosecond laser</a> instead of a mechanical knife. This method is contact-free and does not require sample preparation techniques. The laser microtome has the ability to slice almost every tissue in its native state. Depending on the material being processed, slice thicknesses of 10 to 100 μm are feasible. </p><p><span class="anchor" id="Intervals"></span>Sectioning intervals can be classified mainly into either: </p> <ul><li>Serial sectioning: obtaining a continuous ribbon of sections from a paraffin block and using all for slides.</li> <li>Step sections: collected at specified depths in the block.</li></ul> <div class="mw-heading mw-heading3"><h3 id="Precision_cut_kidney_slices">Precision cut kidney slices</h3><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Microtome&action=edit&section=3" title="Edit section: Precision cut kidney slices"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <p><b>Precision-cut kidney slices</b> refer to thin sections of the kidney tissue that are prepared using a microtome to study kidney functions, drug metabolism or disease processes. Researchers use these slices to study the impact of substances on renal function.<sup id="cite_ref-13" class="reference"><a href="#cite_note-13"><span class="cite-bracket">[</span>13<span class="cite-bracket">]</span></a></sup><sup id="cite_ref-14" class="reference"><a href="#cite_note-14"><span class="cite-bracket">[</span>14<span class="cite-bracket">]</span></a></sup> This includes drug metabolism<sup id="cite_ref-15" class="reference"><a href="#cite_note-15"><span class="cite-bracket">[</span>15<span class="cite-bracket">]</span></a></sup><sup id="cite_ref-16" class="reference"><a href="#cite_note-16"><span class="cite-bracket">[</span>16<span class="cite-bracket">]</span></a></sup> and the effects of toxic substances.<sup id="cite_ref-17" class="reference"><a href="#cite_note-17"><span class="cite-bracket">[</span>17<span class="cite-bracket">]</span></a></sup> </p> <div class="mw-heading mw-heading2"><h2 id="Types">Types</h2><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Microtome&action=edit&section=4" title="Edit section: Types"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <div class="mw-heading mw-heading3"><h3 id="Sledge">Sledge</h3><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Microtome&action=edit&section=5" title="Edit section: Sledge"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <figure class="mw-default-size mw-halign-left" typeof="mw:File/Thumb"><a href="/wiki/File:Sledge_microtome.jpg" class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/9/9f/Sledge_microtome.jpg/220px-Sledge_microtome.jpg" decoding="async" width="220" height="167" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/9/9f/Sledge_microtome.jpg/330px-Sledge_microtome.jpg 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/9/9f/Sledge_microtome.jpg/440px-Sledge_microtome.jpg 2x" data-file-width="576" data-file-height="436" /></a><figcaption>A sled microtome</figcaption></figure> <p>A sledge microtome is a device where the sample is placed into a fixed holder (shuttle), which then moves backwards and forwards across a knife. Modern sled microtomes have the sled placed upon a linear bearing, a design that allows the microtome to readily cut many coarse sections.<sup id="cite_ref-Lang_18-0" class="reference"><a href="#cite_note-Lang-18"><span class="cite-bracket">[</span>18<span class="cite-bracket">]</span></a></sup> By adjusting the angles between the sample and the microtome knife, the pressure applied to the sample during the cut can be reduced.<sup id="cite_ref-Lang_18-1" class="reference"><a href="#cite_note-Lang-18"><span class="cite-bracket">[</span>18<span class="cite-bracket">]</span></a></sup> Typical applications for this design of microtome are of the preparation of large samples, such as those embedded in paraffin for biological preparations. Typical cut thickness achievable on a sledge microtome is between 1 and 60 μm. </p> <div style="clear:both;" class=""></div> <div class="mw-heading mw-heading3"><h3 id="Rotary">Rotary</h3><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Microtome&action=edit&section=6" title="Edit section: Rotary"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <figure class="mw-default-size" typeof="mw:File/Thumb"><a href="/wiki/File:Microtome-1.jpg" class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/6/6f/Microtome-1.jpg/220px-Microtome-1.jpg" decoding="async" width="220" height="246" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/6/6f/Microtome-1.jpg/330px-Microtome-1.jpg 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/6/6f/Microtome-1.jpg/440px-Microtome-1.jpg 2x" data-file-width="1146" data-file-height="1284" /></a><figcaption>A rotary microtome of older construction</figcaption></figure> <p>This instrument is a common microtome design. This device operates with a staged rotary action such that the actual cutting is part of the rotary motion. In a rotary microtome, the knife is typically fixed in a vertical position.<sup id="cite_ref-MVM_19-0" class="reference"><a href="#cite_note-MVM-19"><span class="cite-bracket">[</span>19<span class="cite-bracket">]</span></a></sup> </p> <figure class="mw-default-size mw-halign-left" typeof="mw:File/Thumb"><a href="/wiki/File:Microtome_principle.svg" class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/2/28/Microtome_principle.svg/220px-Microtome_principle.svg.png" decoding="async" width="220" height="200" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/2/28/Microtome_principle.svg/330px-Microtome_principle.svg.png 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/2/28/Microtome_principle.svg/440px-Microtome_principle.svg.png 2x" data-file-width="550" data-file-height="500" /></a><figcaption>Principle of sample movement for making a cut on a rotary microtome</figcaption></figure> <p>In the figure to the left, the principle of the cut is explained. Through the motion of the sample holder, the sample is cut by the knife position 1 to position 2, at which point the fresh section remains on the knife. At the highest point of the rotary motion, the sample holder is advanced by the same thickness as the section that is to be made, allowing the next section to be made. </p><p>The flywheel in many microtomes can be operated by hand. This has the advantage that a clean cut can be made, as the relatively large mass of the flywheel prevents the sample from being stopped during the sample cut. The flywheel in newer models is often integrated inside the microtome casing. The typical cut thickness for a rotary microtome is between 1 and 60 μm. For hard materials, such as a sample embedded in a synthetic resin, this design of microtome can allow good "semi-thin" sections with a thickness of as low as 0.5 μm. </p> <div class="mw-heading mw-heading3"><h3 id="Cryomicrotome">Cryomicrotome</h3><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Microtome&action=edit&section=7" title="Edit section: Cryomicrotome"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r1236090951"><div role="note" class="hatnote navigation-not-searchable">See also: <a href="/wiki/Frozen_section_procedure" title="Frozen section procedure">Frozen section procedure</a></div> <figure class="mw-default-size mw-halign-left" typeof="mw:File/Thumb"><a href="/wiki/File:Cryostat_microtome.jpg" class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/9/9c/Cryostat_microtome.jpg/220px-Cryostat_microtome.jpg" decoding="async" width="220" height="165" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/9/9c/Cryostat_microtome.jpg/330px-Cryostat_microtome.jpg 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/9/9c/Cryostat_microtome.jpg/440px-Cryostat_microtome.jpg 2x" data-file-width="800" data-file-height="600" /></a><figcaption>A cryomicrotome</figcaption></figure> <p>For the cutting of frozen samples, many rotary microtomes can be adapted to cut in a liquid-nitrogen chamber, in a so-called cryomicrotome setup. The reduced temperature allows the hardness of the sample to be increased, such as by undergoing a glass transition, which allows the preparation of semi-thin samples.<sup id="cite_ref-Lang_18-2" class="reference"><a href="#cite_note-Lang-18"><span class="cite-bracket">[</span>18<span class="cite-bracket">]</span></a></sup> However the sample temperature and the knife temperature must be controlled in order to optimise the resultant sample thickness. </p> <div class="mw-heading mw-heading3"><h3 id="Ultramicrotome">Ultramicrotome</h3><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Microtome&action=edit&section=8" title="Edit section: Ultramicrotome"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <figure typeof="mw:File/Thumb"><a href="/wiki/File:Microtome-ultras.jpg" class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/e/e4/Microtome-ultras.jpg/200px-Microtome-ultras.jpg" decoding="async" width="200" height="150" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/e/e4/Microtome-ultras.jpg/300px-Microtome-ultras.jpg 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/e/e4/Microtome-ultras.jpg/400px-Microtome-ultras.jpg 2x" data-file-width="800" data-file-height="600" /></a><figcaption>A ribbon of ultrathin sections prepared by room-temperature ultramicrotomy, floating on water in the boat of a diamond knife used to cut the sections. The knife blade is the edge at the upper end of the trough of water.</figcaption></figure> <p>An ultramicrotome is a main tool of <a href="/wiki/Ultramicrotomy" title="Ultramicrotomy">ultramicrotomy</a>. It allows the preparation of extremely thin sections, with the device functioning in the same manner as a rotational microtome, but with very tight tolerances on the mechanical construction. As a result of the careful mechanical construction, the linear thermal expansion of the mounting is used to provide very fine control of the thickness.<sup id="cite_ref-Lang_18-3" class="reference"><a href="#cite_note-Lang-18"><span class="cite-bracket">[</span>18<span class="cite-bracket">]</span></a></sup> </p><p>These extremely thin cuts are important for use with <a href="/wiki/Transmission_electron_microscope" class="mw-redirect" title="Transmission electron microscope">transmission electron microscope</a> (TEM) and <a href="/wiki/Serial_block-face_scanning_electron_microscopy" title="Serial block-face scanning electron microscopy">serial block-face scanning electron microscopy</a> (SBFSEM), and are sometimes also important for light-optical microscopy.<sup id="cite_ref-MVM_19-1" class="reference"><a href="#cite_note-MVM-19"><span class="cite-bracket">[</span>19<span class="cite-bracket">]</span></a></sup> The typical thickness of these cuts is between 40 and 100 nm for transmission electron microscopy and often between 30 and 50 nm for SBFSEM. Thicker sections up to 500 nm thick are also taken for specialized TEM applications or for light-microscopy survey sections to select an area for the final thin sections. <a href="/wiki/Diamond_knives" class="mw-redirect" title="Diamond knives">Diamond knives</a> (preferably) and glass knives are used with ultramicrotomes. To collect the sections, they are floated on top of a liquid as they are cut and are carefully picked up onto grids suitable for TEM specimen viewing. The thickness of the section can be estimated by the <a href="/wiki/Thin-film_interference" title="Thin-film interference">thin-film interference</a> colors of reflected light that are seen as a result of the extremely low sample thickness.<sup id="cite_ref-20" class="reference"><a href="#cite_note-20"><span class="cite-bracket">[</span>20<span class="cite-bracket">]</span></a></sup> </p> <div class="mw-heading mw-heading3"><h3 id="Vibrating">Vibrating</h3><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Microtome&action=edit&section=9" title="Edit section: Vibrating"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <p>The vibrating microtome operates by cutting using a vibrating blade, allowing the resultant cut to be made with less pressure than would be required for a stationary blade. The vibrating microtome is usually used for difficult biological samples.<sup id="cite_ref-Lang_18-4" class="reference"><a href="#cite_note-Lang-18"><span class="cite-bracket">[</span>18<span class="cite-bracket">]</span></a></sup> The cut thickness is usually around 30–500 μm for live tissue and 10–500 μm for fixed tissue.<sup id="cite_ref-21" class="reference"><a href="#cite_note-21"><span class="cite-bracket">[</span>21<span class="cite-bracket">]</span></a></sup> </p> <div class="mw-heading mw-heading3"><h3 id="Saw">Saw</h3><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Microtome&action=edit&section=10" title="Edit section: Saw"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <p>The saw microtome is especially for hard materials such as teeth or bones. The microtome of this type has a recessed rotating saw, which slices through the sample. The minimal cut thickness is approximately 30 μm and can be made for comparatively large samples.<sup id="cite_ref-Lang_18-5" class="reference"><a href="#cite_note-Lang-18"><span class="cite-bracket">[</span>18<span class="cite-bracket">]</span></a></sup> </p> <div class="mw-heading mw-heading3"><h3 id="Laser">Laser</h3><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Microtome&action=edit&section=11" title="Edit section: Laser"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r1236090951"><div role="note" class="hatnote navigation-not-searchable">See also: <a href="/wiki/Laser_microtome" title="Laser microtome">Laser microtome</a></div> <figure class="mw-default-size" typeof="mw:File/Thumb"><a href="/wiki/File:Laser-microtome-schematic.png" class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/c/cf/Laser-microtome-schematic.png/220px-Laser-microtome-schematic.png" decoding="async" width="220" height="140" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/c/cf/Laser-microtome-schematic.png/330px-Laser-microtome-schematic.png 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/c/cf/Laser-microtome-schematic.png/440px-Laser-microtome-schematic.png 2x" data-file-width="785" data-file-height="501" /></a><figcaption>A conceptual diagram of laser microtome operation</figcaption></figure> <p>The <a href="/wiki/Laser" title="Laser">laser</a> microtome is an instrument for contact-free slicing.<sup id="cite_ref-Lasermicrotome_22-0" class="reference"><a href="#cite_note-Lasermicrotome-22"><span class="cite-bracket">[</span>22<span class="cite-bracket">]</span></a></sup> Prior preparation of the sample through embedding, freezing or chemical <a href="/wiki/Fixation_(histology)" title="Fixation (histology)">fixation</a> is not required, thereby minimizing the artifacts from preparation methods. Alternately this design of microtome can also be used for very hard materials, such as bones or teeth, as well as some ceramics. Dependent upon the properties of the sample material, the thickness achievable is between 10 and 100 μm. </p><p>The device operates using a cutting action of an infrared laser. As the laser emits a radiation in the near infrared, in this wavelength regime the laser can interact with biological materials. Through sharp focusing of the probe within the sample, a focal point of very high intensity, up to <a href="/wiki/Terawatt" class="mw-redirect" title="Terawatt">TW</a>/cm<sup>2</sup>, can be achieved. Through the non-linear interaction of the optical penetration in the focal region a material separation in a process known as photo-disruption is introduced. By limiting the laser pulse durations to the femtoseconds range, the energy expended at the target region is precisely controlled, thereby limiting the interaction zone of the cut to under a micrometre. External to this zone the ultra-short beam application time introduces minimal to no thermal damage to the remainder of the sample. </p><p>The laser radiation is directed onto a fast scanning mirror-based optical system, which allows three-dimensional positioning of the beam crossover, whilst allowing beam traversal to the desired region of interest. The combination of high power with a high raster rate allows the scanner to cut large areas of sample in a short time. In the laser microtome the laser-microdissection of internal areas in tissues, cellular structures, and other types of small features is also possible. </p> <div class="mw-heading mw-heading2"><h2 id="Knives">Knives</h2><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Microtome&action=edit&section=12" title="Edit section: Knives"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <figure class="mw-default-size" typeof="mw:File/Thumb"><a href="/wiki/File:Diamond_Knife_Blade_Edge.jpg" class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/4/4c/Diamond_Knife_Blade_Edge.jpg/220px-Diamond_Knife_Blade_Edge.jpg" decoding="async" width="220" height="165" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/4/4c/Diamond_Knife_Blade_Edge.jpg/330px-Diamond_Knife_Blade_Edge.jpg 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/4/4c/Diamond_Knife_Blade_Edge.jpg/440px-Diamond_Knife_Blade_Edge.jpg 2x" data-file-width="2048" data-file-height="1536" /></a><figcaption>A diamond knife blade used for cutting ultrathin sections (typically 70 to 350 nm) for transmission electron microscopy</figcaption></figure> <figure class="mw-default-size" typeof="mw:File/Thumb"><a href="/wiki/File:%D0%9E%D0%B4%D0%BD%D0%BE%D1%80%D0%B0%D0%B7%D0%BE%D0%B2%D0%BE%D0%B5_%D0%BB%D0%B5%D0%B7%D0%B2%D0%B8%D0%B5_%D0%B4%D0%BB%D1%8F_%D0%BC%D0%B8%D0%BA%D1%80%D0%BE%D1%82%D0%BE%D0%BC%D0%B0.jpg" class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/0/0f/%D0%9E%D0%B4%D0%BD%D0%BE%D1%80%D0%B0%D0%B7%D0%BE%D0%B2%D0%BE%D0%B5_%D0%BB%D0%B5%D0%B7%D0%B2%D0%B8%D0%B5_%D0%B4%D0%BB%D1%8F_%D0%BC%D0%B8%D0%BA%D1%80%D0%BE%D1%82%D0%BE%D0%BC%D0%B0.jpg/220px-%D0%9E%D0%B4%D0%BD%D0%BE%D1%80%D0%B0%D0%B7%D0%BE%D0%B2%D0%BE%D0%B5_%D0%BB%D0%B5%D0%B7%D0%B2%D0%B8%D0%B5_%D0%B4%D0%BB%D1%8F_%D0%BC%D0%B8%D0%BA%D1%80%D0%BE%D1%82%D0%BE%D0%BC%D0%B0.jpg" decoding="async" width="220" height="147" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/0/0f/%D0%9E%D0%B4%D0%BD%D0%BE%D1%80%D0%B0%D0%B7%D0%BE%D0%B2%D0%BE%D0%B5_%D0%BB%D0%B5%D0%B7%D0%B2%D0%B8%D0%B5_%D0%B4%D0%BB%D1%8F_%D0%BC%D0%B8%D0%BA%D1%80%D0%BE%D1%82%D0%BE%D0%BC%D0%B0.jpg/330px-%D0%9E%D0%B4%D0%BD%D0%BE%D1%80%D0%B0%D0%B7%D0%BE%D0%B2%D0%BE%D0%B5_%D0%BB%D0%B5%D0%B7%D0%B2%D0%B8%D0%B5_%D0%B4%D0%BB%D1%8F_%D0%BC%D0%B8%D0%BA%D1%80%D0%BE%D1%82%D0%BE%D0%BC%D0%B0.jpg 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/0/0f/%D0%9E%D0%B4%D0%BD%D0%BE%D1%80%D0%B0%D0%B7%D0%BE%D0%B2%D0%BE%D0%B5_%D0%BB%D0%B5%D0%B7%D0%B2%D0%B8%D0%B5_%D0%B4%D0%BB%D1%8F_%D0%BC%D0%B8%D0%BA%D1%80%D0%BE%D1%82%D0%BE%D0%BC%D0%B0.jpg/440px-%D0%9E%D0%B4%D0%BD%D0%BE%D1%80%D0%B0%D0%B7%D0%BE%D0%B2%D0%BE%D0%B5_%D0%BB%D0%B5%D0%B7%D0%B2%D0%B8%D0%B5_%D0%B4%D0%BB%D1%8F_%D0%BC%D0%B8%D0%BA%D1%80%D0%BE%D1%82%D0%BE%D0%BC%D0%B0.jpg 2x" data-file-width="5448" data-file-height="3632" /></a><figcaption>The cutting edge of a disposable blade for a microtome under a microscope</figcaption></figure> <p>The selection of microtome knife blade profile depends upon the material and preparation of the samples, as well as the final sample requirements (e.g. cut thickness and quality). </p> <div class="mw-heading mw-heading3"><h3 id="Design_and_cut_types">Design and cut types</h3><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Microtome&action=edit&section=13" title="Edit section: Design and cut types"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <figure class="mw-default-size" typeof="mw:File/Thumb"><a href="/wiki/File:Microtome-knife-profile.svg" class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/7/7b/Microtome-knife-profile.svg/220px-Microtome-knife-profile.svg.png" decoding="async" width="220" height="377" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/7/7b/Microtome-knife-profile.svg/330px-Microtome-knife-profile.svg.png 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/7/7b/Microtome-knife-profile.svg/440px-Microtome-knife-profile.svg.png 2x" data-file-width="350" data-file-height="600" /></a><figcaption>Profiles of microtome knives</figcaption></figure> <p>Generally, knives are characterized by the profile of the knife blade, which falls under the categories of planar concave, wedge shaped or chisel shaped designs. </p><p>Planar concave microtome knives are extremely sharp, but are also very delicate and are therefore only used with very soft samples.<sup id="cite_ref-MVM_19-2" class="reference"><a href="#cite_note-MVM-19"><span class="cite-bracket">[</span>19<span class="cite-bracket">]</span></a></sup> The wedge profile knives are somewhat more stable and find use in moderately hard materials, such as in epoxy or cryogenic sample cutting. Finally, the chisel profile with its blunt edge, raises the stability of the knife, whilst requiring significantly more force to achieve the cut. </p><p>For ultramicrotomes, glass and diamond knives are required, the cut breadth of the blade is therefore on the order of a few millimetres and is therefore significantly smaller than for classical microtome knives. Glass knives are usually manufactured by the fracture of glass bars using special "knife-maker" fracturing devices. Glass knives may be used for initial sample preparations even where diamond knives may be used for final sectioning. Glass knives usually have small troughs, made with plastic tape, which are filled with water to allow the sample to float for later collection.<sup id="cite_ref-Lang_18-6" class="reference"><a href="#cite_note-Lang-18"><span class="cite-bracket">[</span>18<span class="cite-bracket">]</span></a></sup> Diamond blades may be built into such an existing trough, allowing for the same collection method. </p> <div class="mw-heading mw-heading3"><h3 id="Sectioning">Sectioning</h3><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Microtome&action=edit&section=14" title="Edit section: Sectioning"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <p>Prior to cutting by microtome, biological materials are usually placed in a more rigid fixative, in a process known as embedding. This is achieved by the inflow of a liquid substance around the sample, such as paraffin (wax) or epoxy, which is placed in a mold and later hardened to produce a "block" which is readily cut. </p><p>The declination is the angle of contact between the sample vertical and knife blade. If the knife blade is at right angles (declination=90) the cut is made directly using a pressure based mode, and the forces are therefore proportionally larger. If the knife is tilted, however, the relative motion of the knife is increasingly parallel to sample motion, allowing for a slicing action. This behaviour is very important for large or hard samples </p><p>The inclination of the knife is the angle between the knife face and the sample. For an optimal result, this angle must be chosen appropriately. The optimal angle depends upon the knife geometry, the cut speed and many other parameters. If the angle is adjusted to zero, the knife cut can often become erratic, and a new location of the knife must be used to smooth this out. </p><p>If the angle is too large, the sample can crumple and the knife can induce periodic thickness variations in the cut. By further increasing the angle such that it is too large one can damage the knife blade itself. </p><p><br /> </p> <div class="mw-heading mw-heading2"><h2 id="See_also">See also</h2><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Microtome&action=edit&section=15" title="Edit section: See also"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <ul><li><a href="/wiki/Histology" title="Histology">Histology</a></li> <li><a href="/wiki/Microscope" title="Microscope">Microscope</a></li> <li><a href="/wiki/Precision_cut_lung_slices" title="Precision cut lung slices">Precision cut lung slices</a></li></ul> <div style="clear:both;" class=""></div> <div class="mw-heading mw-heading2"><h2 id="References">References</h2><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Microtome&action=edit&section=16" title="Edit section: References"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <div class="mw-references-wrap mw-references-columns"><ol class="references"> <li id="cite_note-Hill_1770-1"><span class="mw-cite-backlink">^ <a href="#cite_ref-Hill_1770_1-0"><sup><i><b>a</b></i></sup></a> <a href="#cite_ref-Hill_1770_1-1"><sup><i><b>b</b></i></sup></a></span> <span class="reference-text"><style data-mw-deduplicate="TemplateStyles:r1238218222">.mw-parser-output cite.citation{font-style:inherit;word-wrap:break-word}.mw-parser-output .citation q{quotes:"\"""\"""'""'"}.mw-parser-output .citation:target{background-color:rgba(0,127,255,0.133)}.mw-parser-output .id-lock-free.id-lock-free a{background:url("//upload.wikimedia.org/wikipedia/commons/6/65/Lock-green.svg")right 0.1em center/9px no-repeat}.mw-parser-output .id-lock-limited.id-lock-limited a,.mw-parser-output .id-lock-registration.id-lock-registration a{background:url("//upload.wikimedia.org/wikipedia/commons/d/d6/Lock-gray-alt-2.svg")right 0.1em center/9px no-repeat}.mw-parser-output .id-lock-subscription.id-lock-subscription a{background:url("//upload.wikimedia.org/wikipedia/commons/a/aa/Lock-red-alt-2.svg")right 0.1em center/9px no-repeat}.mw-parser-output .cs1-ws-icon a{background:url("//upload.wikimedia.org/wikipedia/commons/4/4c/Wikisource-logo.svg")right 0.1em center/12px no-repeat}body:not(.skin-timeless):not(.skin-minerva) .mw-parser-output .id-lock-free a,body:not(.skin-timeless):not(.skin-minerva) .mw-parser-output .id-lock-limited a,body:not(.skin-timeless):not(.skin-minerva) .mw-parser-output .id-lock-registration a,body:not(.skin-timeless):not(.skin-minerva) .mw-parser-output .id-lock-subscription a,body:not(.skin-timeless):not(.skin-minerva) .mw-parser-output .cs1-ws-icon a{background-size:contain;padding:0 1em 0 0}.mw-parser-output .cs1-code{color:inherit;background:inherit;border:none;padding:inherit}.mw-parser-output .cs1-hidden-error{display:none;color:var(--color-error,#d33)}.mw-parser-output .cs1-visible-error{color:var(--color-error,#d33)}.mw-parser-output .cs1-maint{display:none;color:#085;margin-left:0.3em}.mw-parser-output .cs1-kern-left{padding-left:0.2em}.mw-parser-output .cs1-kern-right{padding-right:0.2em}.mw-parser-output .citation .mw-selflink{font-weight:inherit}@media screen{.mw-parser-output .cs1-format{font-size:95%}html.skin-theme-clientpref-night .mw-parser-output .cs1-maint{color:#18911f}}@media screen and (prefers-color-scheme:dark){html.skin-theme-clientpref-os .mw-parser-output .cs1-maint{color:#18911f}}</style><cite id="CITEREFHill1770" class="citation book cs1">Hill, John (1770). <a rel="nofollow" class="external text" href="https://archive.org/details/constructiontim00hillgoog"><i>The Construction of Timber, from its early growth; Explained by Microscope, and proven from Experiments, in a great Variety of Kinds</i></a>. London: The author. pp. <a rel="nofollow" class="external text" href="https://archive.org/details/constructiontim00hillgoog/page/n10">5</a>–11, Plate I.</cite><span title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Abook&rft.genre=book&rft.btitle=The+Construction+of+Timber%2C+from+its+early+growth%3B+Explained+by+Microscope%2C+and+proven+from+Experiments%2C+in+a+great+Variety+of+Kinds.&rft.place=London&rft.pages=5-11%2C+Plate+I&rft.pub=The+author&rft.date=1770&rft.aulast=Hill&rft.aufirst=John&rft_id=https%3A%2F%2Farchive.org%2Fdetails%2Fconstructiontim00hillgoog&rfr_id=info%3Asid%2Fen.wikipedia.org%3AMicrotome" class="Z3988"></span></span> </li> <li id="cite_note-2"><span class="mw-cite-backlink"><b><a href="#cite_ref-2">^</a></b></span> <span class="reference-text"><link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r1238218222"><cite id="CITEREFQuekett1848" class="citation book cs1">Quekett, John (1848). <a rel="nofollow" class="external text" href="https://archive.org/details/practicaltreatis00quekuoft"><i>A Practical Treatise on the use of the Microscope</i></a>. London: Hippolyte Bailliere. pp. <a rel="nofollow" class="external text" href="https://archive.org/details/practicaltreatis00quekuoft/page/306">306</a>, Chapter XII (Microtomes and Microtome Knives).</cite><span title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Abook&rft.genre=book&rft.btitle=A+Practical+Treatise+on+the+use+of+the+Microscope&rft.place=London&rft.pages=306%2C+Chapter+XII+%28Microtomes+and+Microtome+Knives%29&rft.pub=Hippolyte+Bailliere&rft.date=1848&rft.aulast=Quekett&rft.aufirst=John&rft_id=https%3A%2F%2Farchive.org%2Fdetails%2Fpracticaltreatis00quekuoft&rfr_id=info%3Asid%2Fen.wikipedia.org%3AMicrotome" class="Z3988"></span></span> </li> <li id="cite_note-Anonymous_1910-3"><span class="mw-cite-backlink"><b><a href="#cite_ref-Anonymous_1910_3-0">^</a></b></span> <span class="reference-text"><link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r1238218222"><cite id="CITEREFAnonymous1910" class="citation journal cs1">Anonymous (1910). <a href="/wiki/File:Anonymous_1910_An_Eighteenth_Century_Microtome.pdf" title="File:Anonymous 1910 An Eighteenth Century Microtome.pdf">"An eighteenth century Microtome"</a>. <i>Journal of the Royal Microscopical Society</i>. 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title="Heavy equipment">equipment</a></div></th></tr><tr><th scope="row" class="navbox-group" style="width:1%"><a href="/wiki/Tree_planting" title="Tree planting">Tree planting</a>,<br /><a href="/wiki/Afforestation" title="Afforestation">afforestation</a></th><td class="navbox-list-with-group navbox-list navbox-odd" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Caulk_boots" title="Caulk boots">Caulk boots</a></li> <li><a href="/wiki/Hoe_(tool)#Types" title="Hoe (tool)">Hoedad</a> (hoedag)</li> <li><a href="/wiki/Groasis_Waterboxx" title="Groasis Waterboxx">Groasis Waterboxx</a></li> <li><a href="/wiki/Mattock" title="Mattock">Mattock</a></li> <li><a href="/wiki/Pottiputki" title="Pottiputki">Pottiputki</a></li> <li><a href="/wiki/Root_trainer" title="Root trainer">Root trainer</a></li> <li><a href="/wiki/Seed_trap" title="Seed trap">Seed trap</a></li> <li><a href="/wiki/Tree_planting_bar" title="Tree planting bar">Tree planting bar</a> (dibble bar)</li> <li><a href="/wiki/Tree_shelter" title="Tree shelter">Tree shelter</a> (Tuley tube)</li> <li><a href="/wiki/Tree_spade" title="Tree spade">Tree spade</a></li></ul> </div></td><td class="noviewer navbox-image" rowspan="7" style="width:1px;padding:0 0 0 2px"><div><span typeof="mw:File"><a href="/wiki/File:Using_a_DTApe.JPG" class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/a/ae/Using_a_DTApe.JPG/70px-Using_a_DTApe.JPG" decoding="async" width="70" height="93" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/a/ae/Using_a_DTApe.JPG/105px-Using_a_DTApe.JPG 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/a/ae/Using_a_DTApe.JPG/140px-Using_a_DTApe.JPG 2x" data-file-width="1536" data-file-height="2048" /></a></span></div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%"><a href="/wiki/Tree_measurement" title="Tree measurement">Mensuration</a></th><td class="navbox-list-with-group navbox-list navbox-even" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/3D_scanning" title="3D scanning">3D scanner</a></li> <li><a href="/wiki/Angle_gauge" title="Angle gauge">Angle gauge</a></li> <li><a href="/wiki/Biltmore_stick" title="Biltmore stick">Biltmore stick</a></li> <li><a href="/wiki/Chain_(unit)#Other_instruments" title="Chain (unit)">Chain</a></li> <li><a href="/wiki/Cruising_rod" title="Cruising rod">Cruising rod</a></li> <li><a href="/wiki/Diameter_tape" title="Diameter tape">Diameter tape</a></li> <li><a href="/wiki/Hemispherical_photography" title="Hemispherical photography">Hemispherical photography</a></li> <li><a href="/wiki/Inclinometer" title="Inclinometer">Inclinometer</a></li> <li><a href="/wiki/Increment_borer" title="Increment borer">Increment borer</a></li> <li><a href="/wiki/Rangefinding_telemeter" class="mw-redirect" title="Rangefinding telemeter">Rangefinder</a> <ul><li><a href="/wiki/Laser_rangefinder" title="Laser rangefinder">laser</a></li></ul></li> <li><a class="mw-selflink selflink">Microtome</a></li> <li><a href="/wiki/Relascope" title="Relascope">Relascope</a></li> <li><a href="/wiki/Tree_caliper" title="Tree caliper">Tree caliper</a></li> <li><a href="/wiki/Wedge_prism" title="Wedge prism">Wedge prism</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%"><a href="/wiki/Wildfire_suppression_equipment_and_personnel" title="Wildfire suppression equipment and personnel">Fire suppression</a></th><td class="navbox-list-with-group navbox-list navbox-odd" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Aerial_firefighting" title="Aerial firefighting">Aerial firefighting</a> <ul><li><a href="/wiki/DC-10_Air_Tanker" title="DC-10 Air Tanker">DC-10</a></li> <li><a href="/wiki/Unmanned_aerial_vehicle#Forest_fire_detection" title="Unmanned aerial vehicle">UAVs</a></li></ul></li> <li><a href="/wiki/Driptorch" title="Driptorch">Driptorch</a></li> <li><a href="/wiki/Fire_flapper_(tool)" class="mw-redirect" title="Fire flapper (tool)">Fire flapper</a></li> <li><a href="/wiki/Fire_rake" title="Fire rake">Fire rake</a></li> <li><a href="/wiki/Fire_retardant#Forest-fire_fighting" title="Fire retardant">Fire retardant</a></li> <li><a href="/wiki/Helitack" title="Helitack">Helitack</a></li> <li><a href="/wiki/McLeod_(tool)" title="McLeod (tool)">McLeod</a> (rakehoe)</li> <li><a href="/wiki/Pulaski_(tool)" title="Pulaski (tool)">Pulaski</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%"><a href="/wiki/Axe" title="Axe">Axes</a></th><td class="navbox-list-with-group navbox-list navbox-even" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Billhook" title="Billhook">Billhook</a></li> <li><a href="/wiki/Broadaxe" title="Broadaxe">Broadaxe</a></li> <li><a href="/wiki/Brush_hook" class="mw-redirect" title="Brush hook">Brush hook</a></li> <li><a href="/wiki/Froe" title="Froe">Froe</a> (shake axe)</li> <li><a href="/wiki/Hatchet" title="Hatchet">Hatchet</a></li> <li><a href="/wiki/Labrys" title="Labrys">Labrys</a></li> <li><a href="/wiki/Log_splitter" title="Log splitter">Log splitter</a></li> <li><a href="/wiki/Marking_axe" title="Marking axe">Marking axe</a></li> <li><a href="/wiki/Splitting_maul" title="Splitting maul">Splitting maul</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%"><a href="/wiki/Saw" title="Saw">Saws</a></th><td class="navbox-list-with-group navbox-list navbox-odd" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Bow_saw" title="Bow saw">Bow saw</a></li> <li><a href="/wiki/Bucksaw" title="Bucksaw">Bucksaw</a></li> <li><a href="/wiki/Chainsaw" title="Chainsaw">Chainsaw</a> <ul><li><a href="/wiki/Chainsaw_safety_clothing" title="Chainsaw safety clothing">safety clothing</a></li> <li><a href="/wiki/Chainsaw_safety_features" title="Chainsaw safety features">safety features</a></li></ul></li> <li><a href="/wiki/Crosscut_saw" title="Crosscut saw">Crosscut saw</a></li> <li><a href="/wiki/Dragsaw" title="Dragsaw">Dragsaw</a></li> <li><a href="/wiki/Head_saw" title="Head saw">Head saw</a></li> <li><a href="/wiki/Lumber_edger" title="Lumber edger">Lumber edger</a></li> <li><a href="/wiki/Polesaw" title="Polesaw">Polesaw</a></li> <li><a href="/wiki/Portable_sawmill" title="Portable sawmill">Portable sawmill</a></li> <li><a href="/wiki/Resaw" title="Resaw">Resaw</a></li> <li><a href="/wiki/Two-man_saw" title="Two-man saw">Two-man saw</a></li> <li><a href="/wiki/Whipsaw" title="Whipsaw">Whipsaw</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%"><a href="/wiki/Logging" title="Logging">Logging</a></th><td class="navbox-list-with-group navbox-list navbox-even" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Cant_hook" title="Cant hook">Cant hook</a></li> <li><a href="/wiki/Feller_buncher" title="Feller buncher">Feller buncher</a></li> <li><a href="/wiki/Forwarder" title="Forwarder">Forwarder</a></li> <li><a href="/wiki/Go-devil" title="Go-devil">Go-devil</a></li> <li><a href="/wiki/Harvester_(forestry)" title="Harvester (forestry)">Harvester</a></li> <li><a href="/wiki/Heli-logging" title="Heli-logging">Helicopter</a></li> <li><a href="/wiki/Logging_truck" title="Logging truck">Log truck</a></li> <li><a href="/wiki/Lombard_Steam_Log_Hauler" title="Lombard Steam Log Hauler">Lombard Steam Log Hauler</a></li> <li><a href="/wiki/Peavey_(tool)" class="mw-redirect" title="Peavey (tool)">Peavey</a></li> <li><a href="/wiki/Pickaroon" title="Pickaroon">Pickaroon</a></li> <li><a href="/wiki/Pike_pole" title="Pike pole">Pike pole</a></li> <li><a href="/wiki/Skid_cone" title="Skid cone">Skid cone</a></li> <li><a href="/wiki/Skidder" title="Skidder">Skidder</a> <ul><li><a href="/wiki/Washington_Winch" title="Washington Winch">Washington Winch</a></li></ul></li> <li><a href="/wiki/Steam_donkey" title="Steam donkey">Steam donkey</a></li> <li><a href="/wiki/Yarder" title="Yarder">Yarder</a> <ul><li><a href="/wiki/Swing_yarder" title="Swing yarder">swing</a></li></ul></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%">Other</th><td class="navbox-list-with-group navbox-list navbox-odd" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Denailer" title="Denailer">Denailer</a></li> <li><a href="/wiki/Firewood_processor" title="Firewood processor">Firewood processor</a></li> <li><a href="/wiki/Forest_railway" title="Forest railway">Forest railway</a></li> <li><a href="/wiki/Forestry_mulching" title="Forestry mulching">Forestry mulcher</a></li> <li><a href="/wiki/Hand_compass" title="Hand compass">Hand compass</a></li> <li><a href="/wiki/Hook_(hand_tool)#Forestry_hook" title="Hook (hand tool)">Hand hook</a></li> <li><a href="/wiki/Hydraulic_debarker" title="Hydraulic debarker">Hydraulic debarker</a></li> <li><a href="/wiki/Log_house_moulder" title="Log house moulder">Log house moulder</a></li> <li><a href="/wiki/Log_pond" title="Log pond">Log pond</a></li> <li><a href="/wiki/Log_flume" title="Log flume">Log flume</a></li> <li><a href="/wiki/Machete" title="Machete">Machete</a></li> <li><a href="/wiki/Stump_grinder" title="Stump grinder">Stump grinder</a></li> <li><a href="/wiki/Chainsaw_mill" title="Chainsaw mill">Chainsaw mill</a></li> <li><a href="/wiki/Tree_baler" title="Tree baler">Tree tyer</a></li> <li><a href="/wiki/Tsakat" class="mw-redirect" title="Tsakat">Tsakat</a></li> <li><a href="/wiki/Whoopie_sling" title="Whoopie sling">Whoopie sling</a></li> <li><a href="/wiki/Kiln#Wood-drying_kiln" title="Kiln">Wood-drying kiln</a></li> <li><a href="/wiki/Woodchipper" title="Woodchipper">Woodchipper</a></li></ul> </div></td></tr><tr><td class="navbox-abovebelow" 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