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Anggraini</title> <meta name="description" content="Search results for: Vivi Anggraini"> <meta name="keywords" content="Vivi Anggraini"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="Vivi Anggraini"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 8</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: Vivi Anggraini</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8</span> Durability of Lime Treated Soil Reinforced by Natural Fibre under Bending Force</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Vivi%20Anggraini">Vivi Anggraini</a>, <a href="https://publications.waset.org/abstracts/search?q=Afshin%20Asadi"> Afshin Asadi</a>, <a href="https://publications.waset.org/abstracts/search?q=Bujang%20B.%20K.%20Huat"> Bujang B. K. Huat</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Earth structures constructed of marine clay soils have tendency to crack. In order to improve the flexural strength and brittleness, a technique of mixing short fibers is introduced to the soil lime mixture. Coir fiber was used in this study as reinforcing elements. An experimental investigation consisting primarily of flexural tensile tests was conducted to examine the influence of coir fibers on the flexural behaviour of the reinforced soils. The test results demonstrated that the coir fibers were effective in improving the flexural strength and young’s modulus of all soils were examined and ductility after peak strength for reinforced marine clay soil was treated by lime. 5% lime treated soil and 1% coir fiber reinforced soil specimen’s demonstrated good strength and durability when submerged in water and retained 45% of their air-cured strengths. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=flexural%20strength" title="flexural strength">flexural strength</a>, <a href="https://publications.waset.org/abstracts/search?q=durabilty" title=" durabilty"> durabilty</a>, <a href="https://publications.waset.org/abstracts/search?q=lime" title=" lime"> lime</a>, <a href="https://publications.waset.org/abstracts/search?q=coir%20fibers" title=" coir fibers"> coir fibers</a>, <a href="https://publications.waset.org/abstracts/search?q=bending%20force" title=" bending force"> bending force</a>, <a href="https://publications.waset.org/abstracts/search?q=ductility" title=" ductility"> ductility</a> </p> <a href="https://publications.waset.org/abstracts/35165/durability-of-lime-treated-soil-reinforced-by-natural-fibre-under-bending-force" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/35165.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">466</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7</span> Virtual Practical Work as Formation of Physics Concept for Student</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sepdiana%20W.%20Rahmawati">Sepdiana W. Rahmawati</a>, <a href="https://publications.waset.org/abstracts/search?q=Santi%20A.%20P.%20Anggraini"> Santi A. P. Anggraini</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The world of education has made progress with the various new technologies with help of computer. No exception physics education, especially virtual physics practical work. By doing practical work, memory of physics concept will be more advantageous for student and they will understand the essence of actual physics, not only spiked formula. With help of computers, created a variety of applications that can be used by students to perform virtual practical work and students will start thinking systematically to be able find its own concepts and understand the application of physics. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=essence%20of%20physics" title="essence of physics">essence of physics</a>, <a href="https://publications.waset.org/abstracts/search?q=formation%20concept" title=" formation concept"> formation concept</a>, <a href="https://publications.waset.org/abstracts/search?q=physics%20concept" title=" physics concept"> physics concept</a>, <a href="https://publications.waset.org/abstracts/search?q=virtual%20practical%20work" title=" virtual practical work"> virtual practical work</a> </p> <a href="https://publications.waset.org/abstracts/39435/virtual-practical-work-as-formation-of-physics-concept-for-student" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/39435.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">406</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> Determining the Effectiveness of Radiation Shielding and Safe Time for Radiation Worker by Employing Monitoring of Accumulation Dose in the Operator Room of CT Scan</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Risalatul%20Latifah">Risalatul Latifah</a>, <a href="https://publications.waset.org/abstracts/search?q=Bunawas%20Bunawas"> Bunawas Bunawas</a>, <a href="https://publications.waset.org/abstracts/search?q=Lailatul%20Muqmiroh"> Lailatul Muqmiroh</a>, <a href="https://publications.waset.org/abstracts/search?q=Anggraini%20D.%20Sensusiati"> Anggraini D. Sensusiati</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Along with the increasing frequency of the use of CT-Scan for radiodiagnostics purposes, it is necessary to study radiation protection. This study examined aspects of radiation protection of workers. This study tried using thermoluminescent dosimeter (TLD) for evaluating radiation shielding and estimating safe time for workers during CT Scan examination. Six TLDs were placed on door, wall, and window inside and outside of the CT Scan room for 1 month. By using TLD monitoring, it could be seen how much radiation was exposed in the operator room. The results showed the effective dose at door, window, and wall was respectively 0.04 mSv, 0.05 mSv, and 0.04 mSv. With these values, it could be evaluated the effectiveness of radiation shielding on doors, glass and walls were respectively 90.6%, 95.5%, and 92.2%. By applying the dose constraint and the estimation of the accumulated dose for one month, radiation workers were still safe to perform the irradiation for 180 patients. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=CT%20scan%20room" title="CT scan room">CT scan room</a>, <a href="https://publications.waset.org/abstracts/search?q=TLD" title=" TLD"> TLD</a>, <a href="https://publications.waset.org/abstracts/search?q=radiation%20worker" title=" radiation worker"> radiation worker</a>, <a href="https://publications.waset.org/abstracts/search?q=dose%20constraint" title=" dose constraint"> dose constraint</a> </p> <a href="https://publications.waset.org/abstracts/63239/determining-the-effectiveness-of-radiation-shielding-and-safe-time-for-radiation-worker-by-employing-monitoring-of-accumulation-dose-in-the-operator-room-of-ct-scan" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/63239.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">288</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> The Role of Substrate-Nozzle Distance in Atomic Nebulizers in the Photoelectrochemical Water Splitting Performance of ZnO Nanorods</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Lukman%20Andi%20Priyatna">Lukman Andi Priyatna</a>, <a href="https://publications.waset.org/abstracts/search?q=Vivi%20Fauzia"> Vivi Fauzia</a>, <a href="https://publications.waset.org/abstracts/search?q=Ferry%20Anggoro%20Ardy%20Nugroho"> Ferry Anggoro Ardy Nugroho</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Zinc oxide (ZnO) based nanostructures are ubiquitous in applications due to their favourable physicochemical properties and ease of fabrication. One widely accessible route to synthesize ZnO nanorods, which show promising performance in e.g. photoelectrochemical water splitting, is hydrothermal growth of ZnO seeds, obtained via an atomic nebulizer. Despite its popularity, study on the impact of the synthesis parameters in atomic nebulizer on the performance of the synthesized ZnO nanostructures is lacking. This study presents an investigation on the impact of the distance between substrates and atomic nebulizer nozzle on the photoelectrochemical water splitting performance of ZnO nanorods. Adjusting such a distance reveals an optimum separation which results in nanostructures with highest absorbance. Such high absorbance translates into improved photoelectrochemistry, as evaluated by higher photocurrent density, from 0.11 mA/cm² to 0.14 mA/cm² and higher Applied Bias Photon-to-Current Efficiency (ABPE) from 0.12% to 0.14%. These results underscore the importance of understanding and optimizing the experimental parameters during ZnO nanostructure synthesis. In a broader context, it advertises the need to carefully assess the corresponding fabrication parameters to optimize the performance of the obtained nanostructures. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=atomic%20nebulizer" title="atomic nebulizer">atomic nebulizer</a>, <a href="https://publications.waset.org/abstracts/search?q=photocurrent%20density" title=" photocurrent density"> photocurrent density</a>, <a href="https://publications.waset.org/abstracts/search?q=photoelectrochemical%20water%20splitting" title=" photoelectrochemical water splitting"> photoelectrochemical water splitting</a>, <a href="https://publications.waset.org/abstracts/search?q=ZnO%20nanorods" title=" ZnO nanorods"> ZnO nanorods</a> </p> <a href="https://publications.waset.org/abstracts/190248/the-role-of-substrate-nozzle-distance-in-atomic-nebulizers-in-the-photoelectrochemical-water-splitting-performance-of-zno-nanorods" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/190248.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">31</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Techno-Economic Study on the Potential of Dimethyl Ether (DME) as a Substitute for LPG</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Widya%20Anggraini%20Pamungkas">Widya Anggraini Pamungkas</a>, <a href="https://publications.waset.org/abstracts/search?q=Rosana%20Budi%20Setyawati"> Rosana Budi Setyawati</a>, <a href="https://publications.waset.org/abstracts/search?q=Awaludin%20Fitroh%20Rifai"> Awaludin Fitroh Rifai</a>, <a href="https://publications.waset.org/abstracts/search?q=Candra%20Pangesti%20Setiawan"> Candra Pangesti Setiawan</a>, <a href="https://publications.waset.org/abstracts/search?q=Anatta%20Wahyu%20Budiiman"> Anatta Wahyu Budiiman</a>, <a href="https://publications.waset.org/abstracts/search?q=Inayati"> Inayati</a>, <a href="https://publications.waset.org/abstracts/search?q=Joko%20Waluyo"> Joko Waluyo</a>, <a href="https://publications.waset.org/abstracts/search?q=Sunu%20Herwi%20Pranolo"> Sunu Herwi Pranolo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The increase in LPG consumption in Indonesia is not balanced with the amount of supply. The high demand for LPG due to the success of the government's kerosene-to-LPG conversion program and the Covid-19 pandemic in 2020 led to an increase in LPG consumption in the household sector and caused Indonesia's trade balance to experience a deficit. The high consumption of LPG encourages the need for alternative fuels as a substitute or which aims to substitute LPG; one of the materials that can be used is Dimethyl Ether (DME). Dimethyl ether (DME) is an organic compound with the chemical formula CH 3. OCH 3 has a high cetane number and has characteristics similar to LPG. DME can be produced from various sources, such as coal, biomass and natural gas. Based on the economic analysis conducted at 10% IRR, coal has the largest NPV of Rp. 20,034,837,497,241 with a payback period of 3.86 years, then biomass with an NPV of Rp. 10,401,526,072,850 and a payback period of 5.16. the latter is natural gas with an NPV of IDR 7,401,272,559,191 and a payback period of 6.17 years. Of the three sources of raw materials used, if the sensitivity is calculated using the selling price of DME equal to the selling price of LPG, it will get an NPV value that is greater than the NPV value when using the current DME price. The advantages of coal as a raw material for DME are not only because it is profitable, namely: low price and abundant resources, but has high greenhouse gas emissions. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=LPG" title="LPG">LPG</a>, <a href="https://publications.waset.org/abstracts/search?q=DME" title=" DME"> DME</a>, <a href="https://publications.waset.org/abstracts/search?q=coal" title=" coal"> coal</a>, <a href="https://publications.waset.org/abstracts/search?q=biomass" title=" biomass"> biomass</a>, <a href="https://publications.waset.org/abstracts/search?q=natural%20gas" title=" natural gas"> natural gas</a> </p> <a href="https://publications.waset.org/abstracts/161115/techno-economic-study-on-the-potential-of-dimethyl-ether-dme-as-a-substitute-for-lpg" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/161115.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">123</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Cilubaba: An Agriculture-Based Education Tool through Congklak Traditional Game as an Introduction of Home Garden for Children in Cibanteng, Bogor</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yoni%20Elviandri">Yoni Elviandri</a>, <a href="https://publications.waset.org/abstracts/search?q=Vivi%20Fitriyanti"> Vivi Fitriyanti</a>, <a href="https://publications.waset.org/abstracts/search?q=Agung%20Surya%20Wijaya"> Agung Surya Wijaya</a>, <a href="https://publications.waset.org/abstracts/search?q=Suryani%20Humayyah"> Suryani Humayyah</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Alif%20Azizi"> Muhammad Alif Azizi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The massive development of computing power and internet access nowadays is marked by audiovisual games and computers which are known as electronic games, one of the examples is online games. This kind of game can be found everywhere in Indonesia, both in the cities and even the villages. In the present time, online games are becoming a popular games in various layers of the community, one of them does happen to elementary school students. As the online games spread over, the traditional games gradually fade away and even thought as an old-fashioned game. Contrary, traditional games actually have the better and higher educational values such as patience, honesty, integrity and togetherness value which cannot be found in online games which are more to individualist. A brand new set of education tools is necessary to provide a convenience, safe and fun place for children to play around but still contains educational values. One interesting example goes to Cilulaba is an agricultural-based playground. It is a good place for children to play and learn as it was planned to entertain children to play around as well as introducing agriculture to them. One of the games is a 1990’s well-known traditional game which its name is Congklak. Congklak is an agricultural-based traditional game and it also introduces the home garden to the children. Some of the Cilulaba’s aims are to protect the existence of nation’s cultural inheritance through Congklak traditional game, as a tool to introduce the agriculture to the children through the methods of Congklak traditional game and giving explanation related to the advantages of a “healthy home garden” to the children. The expected output from this place is to deliver a good understanding about agriculture to the children and make them begin to love it to make an aesthetic home garden and enhance the optimalisation usage of home garden that will support the availability of various edible plants in productive and health households. The proposed method in this Student Creative Program in Society Service is Participatory Rural Appraisal (PRA) method. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Cilubaba" title="Cilubaba">Cilubaba</a>, <a href="https://publications.waset.org/abstracts/search?q=Congklak" title=" Congklak"> Congklak</a>, <a href="https://publications.waset.org/abstracts/search?q=traditional%20game" title=" traditional game"> traditional game</a>, <a href="https://publications.waset.org/abstracts/search?q=agricultural-based%20playground" title=" agricultural-based playground"> agricultural-based playground</a> </p> <a href="https://publications.waset.org/abstracts/3777/cilubaba-an-agriculture-based-education-tool-through-congklak-traditional-game-as-an-introduction-of-home-garden-for-children-in-cibanteng-bogor" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/3777.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">441</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Establishment and Aging Process Analysis in Dermal Fibroblast Cell Culture of Green Turtle (Chelonia mydas)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yemima%20Dani%20Riani">Yemima Dani Riani</a>, <a href="https://publications.waset.org/abstracts/search?q=Anggraini%20Barlian"> Anggraini Barlian</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Green turtle (Chelonia mydas) is one of well known long-lived turtle. Its age can reach 100 years old. Senescence in green turtle is an interesting process to study because until now no clear explanation has been established about senescence at cellular or molecular level in this species. Since 1999, green turtle announced as an endangered species. Hence, establishment of fibroblast skin cell culture of green turtle may be material for future study of senescence. One common marker used for detecting senescence is telomere shortening. Reduced telomerase activity, the reverse transcriptase enzyme which adds TTAGGG DNA sequence to telomere end, may also cause senescence. The purpose of this research are establish and identify green turtle fibroblast skin cell culture and also compare telomere length and telomerase activity from passage 5 and 14. Primary cell culture made with primary explant method then cultured in Leibovitz-15 (Sigma) supplemented by 10% Fetal Bovine Serum (Sigma) and 100 U/mL Penicillin/Streptomycin (Sigma) at 30 ± 1oC. Cells identified with Rabbit Anti-Vimentin Polyclonal Antibody (Abcam) and Goat Polyclonal Antibody (Abcam) using confocal microscope (Zeiss LSM 170). Telomere length obtained using TeloTAGGG Telomere Length Assay (Roche) while telomerase activity obtained using TeloTAGGG Telomerase PCR ElisaPlus (Roche). Primary cell culture from green turtle skin had fibroblastic morphology and immunocytochemistry test with vimentin antibody proved the culture was fibroblast cell. Measurement of telomere length and telomerase activity showed that telomere length and telomerase activity of passage 14 was greater than passage 5. However, based on morphology, green turtle fibroblast skin cell culture showed senescent morphology. Based on the analysis of telomere length and telomerase activity, suspected fibroblast skin cell culture of green turtles is not undergo aging through telomere shortening. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cell%20culture" title="cell culture">cell culture</a>, <a href="https://publications.waset.org/abstracts/search?q=chelonia%20mydas" title=" chelonia mydas"> chelonia mydas</a>, <a href="https://publications.waset.org/abstracts/search?q=telomerase" title=" telomerase"> telomerase</a>, <a href="https://publications.waset.org/abstracts/search?q=telomere" title=" telomere"> telomere</a>, <a href="https://publications.waset.org/abstracts/search?q=senescence" title=" senescence"> senescence</a> </p> <a href="https://publications.waset.org/abstracts/15170/establishment-and-aging-process-analysis-in-dermal-fibroblast-cell-culture-of-green-turtle-chelonia-mydas" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/15170.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">425</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Wt1 and FoxL2 Genes Expression Pattern in Mesonephros-Gonad Complexes of Green Sea Turtle (Chelonia mydas) Embryos Incubated in Feminization and Masculinization Temperature</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Fitria%20D.%20Ayuningtyas">Fitria D. Ayuningtyas</a>, <a href="https://publications.waset.org/abstracts/search?q=Anggraini%20Barlian"> Anggraini Barlian</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Green turtle (Chelonia mydas) is one of TSD (Temperature-dependent Sex Determination, TSD) animals which sex is determined by the egg’s incubation temperature. GSD (Genotypic Sex Determination) homologous genes such as Wilms’ Tumor (Wt1) and Forkhead Box L2 (FoxL2) play a role in TSD animal sex determination process. Wt1 plays a role in both male pathway, as a transcription factor for Sf1 gene and in female pathway, as a transcription factor for Dax1. FoxL2 plays a role specifically in female sex determination, and known as transcriptional factor for Aromatase gene. Until now, research on the pattern of Wt1 and FoxL2 genes expression in C.mydas has not been conducted yet. The aim of this research is to know the pattern of Wt1 and FoxL2 genes expression in Mesonephros-Gonad (MG) complexes of Chelonia mydas embryos incubated in masculinizing temperature (MT) and feminizing temperature (FT). Eggs of C.mydas incubated in 3 different stage of TSP (Thermosensitive Period) at masculinizing temperature (26±10C, MT) and feminizing temperature (31±10C FT). Mesonefros-gonad complexes were isolated at Pre-TSP stage (FT at days 14th, MT at days 24th), TSP stage (FT at days 24th, MT at days 36th) and differentiated stage (FT at days 40th, MT at days 58th). RNA from mesonephros-gonad (MG) complexes were converted into cDNA by RT-PCR process, and the pattern of Wt1 and FoxL2 genes expression is analyzed by quantitative Real Time PCR (qPCR) method, β-actin gene is used as an internal control. The pattern of Wt1 gene expression in Pre-TSP stage was almost the same between MG complexes incubated at MT or FT, while TSP and differentiation stage, the pattern of Wt1 gene expression in MG complexes incubated at MT or FT was increased. Wt1 gene expression of MG complexes that incubated at FT was higher than at MT. There was a difference pattern between Wt1 gene expression in this research compared to the previous research in protein level. It could be assumed that the difference caused by post-transcriptional regulation mechanisms before mRNA of Wt1 gene translated into protein structure. The pattern of FoxL2 gene expression in Pre-TSP stage was almost the same between MG complexes that incubated at MT and FT, and increased in both TSP and differentiated stage. The FoxL2 gene expression in MG complexes that incubated in FT is higher than MT on TSP and differentiated stage. Based on the results of this research, it can be assumed that Wt1 and FoxL2 gene were expressed in MG complexes that incubated both at MT and FT since Pre-TSP stage. The pattern of Wt1 gene expression was increased in every stage of gonadal development, and so do the pattern of FoxL2 gene expression. Wt1 and FoxL2 gene expressions were higher in MG complexes incubated at FT than MT. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=chelonia%20mydas" title="chelonia mydas">chelonia mydas</a>, <a href="https://publications.waset.org/abstracts/search?q=FoxL2" title=" FoxL2"> FoxL2</a>, <a href="https://publications.waset.org/abstracts/search?q=gene%20expression" title=" gene expression"> gene expression</a>, <a href="https://publications.waset.org/abstracts/search?q=TSD" title=" TSD"> TSD</a>, <a href="https://publications.waset.org/abstracts/search?q=Wt1" title=" Wt1"> Wt1</a> </p> <a href="https://publications.waset.org/abstracts/15175/wt1-and-foxl2-genes-expression-pattern-in-mesonephros-gonad-complexes-of-green-sea-turtle-chelonia-mydas-embryos-incubated-in-feminization-and-masculinization-temperature" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/15175.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">407</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Explore <li><a href="https://waset.org/disciplines">Disciplines</a></li> <li><a href="https://waset.org/conferences">Conferences</a></li> <li><a href="https://waset.org/conference-programs">Conference Program</a></li> <li><a href="https://waset.org/committees">Committees</a></li> <li><a href="https://publications.waset.org">Publications</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Research <li><a href="https://publications.waset.org/abstracts">Abstracts</a></li> <li><a href="https://publications.waset.org">Periodicals</a></li> <li><a href="https://publications.waset.org/archive">Archive</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Open Science <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Philosophy.pdf">Open Science Philosophy</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Award.pdf">Open Science Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Society-Open-Science-and-Open-Innovation.pdf">Open Innovation</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Postdoctoral-Fellowship-Award.pdf">Postdoctoral Fellowship Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Scholarly-Research-Review.pdf">Scholarly Research Review</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Support <li><a href="https://waset.org/page/support">Support</a></li> <li><a href="https://waset.org/profile/messages/create">Contact Us</a></li> <li><a href="https://waset.org/profile/messages/create">Report Abuse</a></li> </ul> </div> </div> </div> </div> </div> <div class="container text-center"> <hr style="margin-top:0;margin-bottom:.3rem;"> <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" class="text-muted small">Creative Commons Attribution 4.0 International License</a> <div id="copy" class="mt-2">© 2024 World Academy of Science, Engineering and Technology</div> </div> </footer> <a href="javascript:" id="return-to-top"><i class="fas fa-arrow-up"></i></a> <div class="modal" id="modal-template"> <div class="modal-dialog"> <div class="modal-content"> <div class="row m-0 mt-1"> <div class="col-md-12"> <button type="button" class="close" data-dismiss="modal" aria-label="Close"><span aria-hidden="true">×</span></button> </div> </div> <div class="modal-body"></div> </div> </div> </div> <script src="https://cdn.waset.org/static/plugins/jquery-3.3.1.min.js"></script> <script src="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/js/bootstrap.bundle.min.js"></script> <script src="https://cdn.waset.org/static/js/site.js?v=150220211556"></script> <script> jQuery(document).ready(function() { /*jQuery.get("https://publications.waset.org/xhr/user-menu", function (response) { jQuery('#mainNavMenu').append(response); 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