CINXE.COM

<!DOCTYPE html> <html lang="en" dir="ltr"> <head>  <script async src="https://www.googletagmanager.com/gtag/js?id=G-P63WKM1TM1"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-P63WKM1TM1'); </script>  <script type="text/javascript" > (function(m,e,t,r,i,k,a){m[i]=m[i]||function(){(m[i].a=m[i].a||[]).push(arguments)}; m[i].l=1*new Date(); for (var j = 0; j < document.scripts.length; j++) {if (document.scripts[j].src === r) { return; }} k=e.createElement(t),a=e.getElementsByTagName(t)[0],k.async=1,k.src=r,a.parentNode.insertBefore(k,a)}) (window, document, "script", "https://mc.yandex.ru/metrika/tag.js", "ym"); ym(55165297, "init", { clickmap:false, trackLinks:true, accurateTrackBounce:true, webvisor:false }); </script> <noscript><div><img src="https://mc.yandex.ru/watch/55165297" style="position:absolute; left:-9999px;" alt="" /></div></noscript>    <title>Search results for: Growth Hormone Gene</title> <meta name="description" content="Search results for: Growth Hormone Gene"> <meta name="keywords" content="Growth Hormone Gene"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" type="button" data-toggle="collapse" data-target="#navbarMenu" aria-controls="navbarMenu" aria-expanded="false" aria-label="Toggle navigation"> <span class="navbar-toggler-icon"></span> </button> <div class="w-100"> <div class="d-none d-lg-flex flex-row-reverse"> <form method="get" action="https://waset.org/search" class="form-inline my-2 my-lg-0"> <input class="form-control mr-sm-2" type="search" placeholder="Search Conferences" value="Growth Hormone Gene" name="q" aria-label="Search"> <button class="btn btn-light my-2 my-sm-0" type="submit"><i class="fas fa-search"></i></button> </form> </div> <div class="collapse navbar-collapse mt-1" id="navbarMenu"> <ul class="navbar-nav ml-auto align-items-center" id="mainNavMenu"> <li class="nav-item"> <a class="nav-link" href="https://waset.org/conferences" title="Conferences in 2024/2025/2026">Conferences</a> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/disciplines" title="Disciplines">Disciplines</a> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/committees" rel="nofollow">Committees</a> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdownPublications" role="button" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Publications </a> <div class="dropdown-menu" aria-labelledby="navbarDropdownPublications"> <a class="dropdown-item" href="https://publications.waset.org/abstracts">Abstracts</a> <a class="dropdown-item" href="https://publications.waset.org">Periodicals</a> <a class="dropdown-item" href="https://publications.waset.org/archive">Archive</a> </div> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/page/support" title="Support">Support</a> </li> </ul> </div> </div> </nav> </div> </header> <main> <div class="container mt-4"> <div class="row"> <div class="col-md-9 mx-auto"> <form method="get" action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="Growth Hormone Gene"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 7871</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: Growth Hormone Gene</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7661</span> Recognition of Gene Names from Gene Pathway Figures Using Siamese Network</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Azam">Muhammad Azam</a>, <a href="https://publications.waset.org/abstracts/search?q=Micheal%20Olaolu%20Arowolo"> Micheal Olaolu Arowolo</a>, <a href="https://publications.waset.org/abstracts/search?q=Fei%20He"> Fei He</a>, <a href="https://publications.waset.org/abstracts/search?q=Mihail%20Popescu"> Mihail Popescu</a>, <a href="https://publications.waset.org/abstracts/search?q=Dong%20Xu"> Dong Xu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The number of biological papers is growing quickly, which means that the number of biological pathway figures in those papers is also increasing quickly. Each pathway figure shows extensive biological information, like the names of genes and how the genes are related. However, manually annotating pathway figures takes a lot of time and work. Even though using advanced image understanding models could speed up the process of curation, these models still need to be made more accurate. To improve gene name recognition from pathway figures, we applied a Siamese network to map image segments to a library of pictures containing known genes in a similar way to person recognition from photos in many photo applications. We used a triple loss function and a triplet spatial pyramid pooling network by combining the triplet convolution neural network and the spatial pyramid pooling (TSPP-Net). We compared VGG19 and VGG16 as the Siamese network model. VGG16 achieved better performance with an accuracy of 93%, which is much higher than OCR results. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biological%20pathway" title="biological pathway">biological pathway</a>, <a href="https://publications.waset.org/abstracts/search?q=image%20understanding" title=" image understanding"> image understanding</a>, <a href="https://publications.waset.org/abstracts/search?q=gene%20name%20recognition" title=" gene name recognition"> gene name recognition</a>, <a href="https://publications.waset.org/abstracts/search?q=object%20detection" title=" object detection"> object detection</a>, <a href="https://publications.waset.org/abstracts/search?q=Siamese%20network" title=" Siamese network"> Siamese network</a>, <a href="https://publications.waset.org/abstracts/search?q=VGG" title=" VGG"> VGG</a> </p> <a href="https://publications.waset.org/abstracts/160723/recognition-of-gene-names-from-gene-pathway-figures-using-siamese-network" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/160723.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">291</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7660</span> Expression Level of Dehydration-Responsive Element Binding/DREB Gene of Some Local Corn Cultivars from Kisar Island-Maluku Indonesia Using Quantitative Real-Time PCR</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hermalina%20Sinay">Hermalina Sinay</a>, <a href="https://publications.waset.org/abstracts/search?q=Estri%20L.%20Arumingtyas"> Estri L. Arumingtyas</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The research objective was to determine the expression level of dehydration responsive element binding/DREB gene of local corn cultivars from Kisar Island Maluku. The study design was a randomized block design with single factor consist of six local corn cultivars obtained from farmers in Kisar Island and one reference varieties wich has been released by the government as a drought-tolerant varieties and obtained from Cereal Crops Research Institute (ICERI) Maros South Sulawesi. Leaf samples were taken is the second leaf after the flag leaf at the 65 days after planting. Isolation of total RNA from leaf samples was carried out according to the protocols of the R & A-BlueTM Total RNA Extraction Kit and was used as a template for cDNA synthesis. The making of cDNA from total RNA was carried out according to the protocol of One-Step Reverse Transcriptase PCR Premix Kit. Real Time-PCR was performed on cDNA from reverse transcription followed the procedures of Real MODTM Green Real-Time PCR Master Mix Kit. Data obtained from the real time-PCR results were analyzed using relative quantification method based on the critical point / Cycle Threshold (CP / CT). The results of gene expression analysis of DREB gene showed that the expression level of the gene was highest obtained at Deep Yellow local corn cultivar, and the lowest one was obtained at the Rubby Brown Cob cultivar. It can be concluded that the expression level of DREB gene of Deep Yellow local corn cultivar was highest than other local corn cultivars and Srikandi variety as a reference variety. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=expression" title="expression">expression</a>, <a href="https://publications.waset.org/abstracts/search?q=level" title=" level"> level</a>, <a href="https://publications.waset.org/abstracts/search?q=DREB%20gene" title=" DREB gene"> DREB gene</a>, <a href="https://publications.waset.org/abstracts/search?q=local%20corn%20cultivars" title=" local corn cultivars"> local corn cultivars</a>, <a href="https://publications.waset.org/abstracts/search?q=Kisar%20Island" title=" Kisar Island"> Kisar Island</a>, <a href="https://publications.waset.org/abstracts/search?q=Maluku" title=" Maluku"> Maluku</a> </p> <a href="https://publications.waset.org/abstracts/47435/expression-level-of-dehydration-responsive-element-bindingdreb-gene-of-some-local-corn-cultivars-from-kisar-island-maluku-indonesia-using-quantitative-real-time-pcr" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/47435.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">299</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7659</span> Insights into Archaeological Human Sample Microbiome Using 16S rRNA Gene Sequencing</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alisa%20Kazarina">Alisa Kazarina</a>, <a href="https://publications.waset.org/abstracts/search?q=Guntis%20Gerhards"> Guntis Gerhards</a>, <a href="https://publications.waset.org/abstracts/search?q=Elina%20Petersone-Gordina"> Elina Petersone-Gordina</a>, <a href="https://publications.waset.org/abstracts/search?q=Ilva%20Pole"> Ilva Pole</a>, <a href="https://publications.waset.org/abstracts/search?q=Viktorija%20Igumnova"> Viktorija Igumnova</a>, <a href="https://publications.waset.org/abstracts/search?q=Janis%20Kimsis"> Janis Kimsis</a>, <a href="https://publications.waset.org/abstracts/search?q=Valentina%20Capligina"> Valentina Capligina</a>, <a href="https://publications.waset.org/abstracts/search?q=Renate%20Ranka"> Renate Ranka</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Human body is inhabited by a vast number of microorganisms, collectively known as the human microbiome, and there is a tremendous interest in evolutionary changes in human microbial ecology, diversity and function. The field of paleomicrobiology, study of ancient human microbiome, is powered by modern techniques of Next Generation Sequencing (NGS), which allows extracting microbial genomic data directly from archaeological sample of interest. One of the major techniques is 16S rRNA gene sequencing, by which certain 16S rRNA gene hypervariable regions are being amplified and sequenced. However, some limitations of this method exist including the taxonomic precision and efficacy of different regions used. The aim of this study was to evaluate the phylogenetic sensitivity of different 16S rRNA gene hypervariable regions for microbiome studies in the archaeological samples. Towards this aim, archaeological bone samples and corresponding soil samples from each burial environment were collected in Medieval cemeteries in Latvia. The Ion 16S™ Metagenomics Kit targeting different 16S rRNA gene hypervariable regions was used for library construction (Ion Torrent technologies). Sequenced data were analysed by using appropriate bioinformatic techniques; alignment and taxonomic representation was done using Mothur program. Sequences of most abundant genus were further aligned to E. coli 16S rRNA gene reference sequence using MEGA7 in order to identify the hypervariable region of the segment of interest. Our results showed that different hypervariable regions had different discriminatory power depending on the groups of microbes, as well as the nature of samples. On the basis of our results, we suggest that wider range of primers used can provide more accurate recapitulation of microbial communities in archaeological samples. Acknowledgements. This work was supported by the ERAF grant Nr. 1.1.1.1/16/A/101. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=16S%20rRNA%20gene" title="16S rRNA gene">16S rRNA gene</a>, <a href="https://publications.waset.org/abstracts/search?q=ancient%20human%20microbiome" title=" ancient human microbiome"> ancient human microbiome</a>, <a href="https://publications.waset.org/abstracts/search?q=archaeology" title=" archaeology"> archaeology</a>, <a href="https://publications.waset.org/abstracts/search?q=bioinformatics" title=" bioinformatics"> bioinformatics</a>, <a href="https://publications.waset.org/abstracts/search?q=genomics" title=" genomics"> genomics</a>, <a href="https://publications.waset.org/abstracts/search?q=microbiome" title=" microbiome"> microbiome</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20biology" title=" molecular biology"> molecular biology</a>, <a href="https://publications.waset.org/abstracts/search?q=next-generation%20sequencing" title=" next-generation sequencing"> next-generation sequencing</a> </p> <a href="https://publications.waset.org/abstracts/78646/insights-into-archaeological-human-sample-microbiome-using-16s-rrna-gene-sequencing" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/78646.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">190</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7658</span> Expression of Selected miRNAs in Placenta of the Intrauterine Restricted Growth Fetuses in Cattle</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Karolina%20Rutkowska">Karolina Rutkowska</a>, <a href="https://publications.waset.org/abstracts/search?q=Hubert%20Pausch"> Hubert Pausch</a>, <a href="https://publications.waset.org/abstracts/search?q=Jolanta%20Oprzadek"> Jolanta Oprzadek</a>, <a href="https://publications.waset.org/abstracts/search?q=Krzysztof%20Flisikowski"> Krzysztof Flisikowski</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The placenta is one of the most important organs that plays a crucial role in the fetal growth and development. Placenta dysfunction is one of the primary cause of the intrauterine growth restriction (IUGR). Cattle have the cotyledonary placenta which consists of two anatomical parts: fetal and maternal. In the case of cattle during the first months of pregnancy, it is very easy to separate maternal caruncle from fetal cotyledon tissue, easier in fact than removing an ordinary glove from one's hand. Which in fact make easier to conduct tissue-specific molecular studies. Typically, animal models for the study of IUGR are created using surgical methods and malnutrition of the pregnant mother or in the case of mice by genetic modifications. However, proposed cattle model with MIMT1Del/WT deletion is unique because it was created without any surgical methods what significantly distinguish it from the other animal models. The primary objective of the study was to identify differential expression of selected miRNAs in the placenta from normal and intrauterine growth restricted fetuses. There was examined the expression of miRNA in the fetal and maternal part of the placenta from 24 fetuses (12 samples from the fetal part of the placenta and 12 samples from maternal part of the placenta). In the study, there was done miRNAs sequencing in the placenta of MIMT1Del/WT fetuses and MIMT1WT/WT fetuses. Then, there were selected miRNAs that are involved in fetal growth and development. Analysis of miRNAs expression was conducted on ABI7500 machine. miRNAs expression was analyzed by reverse-transcription polymerase chain reaction (RT-PCR). As the reference gene was used SNORD47. The results were expressed as 2ΔΔCt: ΔΔCt = (Ctij − CtSNORD47j) − (Cti1 − CtSNORD471). Where Ctij and CtSNORD47j are the Ct values for gene i and for SNORD47 in a sample (named j); Cti1 and CtSNORD471 are the Ct values in sample 1. Differences between groups were evaluated with analysis of variance by using One-Way ANOVA. Bonferroni’s tests were used for interpretation of the data. All normalised miRNA expression values are expressed on a value of natural logarithm. The data were expressed as least squares mean with standard errors. Significance was declared when P < 0.05. The study shows that miRNAs expression depends on the part of the placenta where they origin (fetal or maternal) and on the genotype of the animal. miRNAs offer a particularly new approach to study IUGR. Corresponding tissue samples were collected according to the standard veterinary protocols according to the European Union Normative for Care and Use of Experimental Animals. All animal experiments were approved by the Animal Ethics Committee of the State Provincial Office of Southern Finland (ESAVI-2010-08583/YM-23). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=placenta" title="placenta">placenta</a>, <a href="https://publications.waset.org/abstracts/search?q=intrauterine%20growth%20restriction" title=" intrauterine growth restriction"> intrauterine growth restriction</a>, <a href="https://publications.waset.org/abstracts/search?q=miRNA" title=" miRNA"> miRNA</a>, <a href="https://publications.waset.org/abstracts/search?q=cattle" title=" cattle"> cattle</a> </p> <a href="https://publications.waset.org/abstracts/64272/expression-of-selected-mirnas-in-placenta-of-the-intrauterine-restricted-growth-fetuses-in-cattle" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/64272.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">314</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7657</span> Analysis of ZBTB17 Gene rs10927875 Polymorphism in Relation to Dilated Cardiomyopathy in Slovak Population</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=I.%20Boro%C5%88ov%C3%A1">I. Boroňová</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Bernasovsk%C3%A1"> J. Bernasovská</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Kmec"> J. Kmec</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20Petrej%C4%8D%C3%ADkov%C3%A1"> E. Petrejčíková</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Dilated cardiomyopathy (DCM) is a primary myocardial disease, it is characterized by progressive systolic dysfunction due to cardiac chamber dilatation and inefficient myocardial contractility with estimated prevalence of 37 in 100 000 people. It is the most frequent cause of heart failure and cardiac transplantation in young adults. About one-third of all patients have a suspected familial disease indicating a genetic basis of DCM. Many candidate gene studies in humans have tested the association of single nucleotide polymorphisms (SNPs) in various genes coding for proteins with a known cardiovascular function. In our study we present the results of ZBTB17 gene rs10927875 polymorphism genotyping in relation to dilated cardiomyopathy in Slovak population. The study included 78 individuals, 39 patients with DCM and 39 healthy control persons. The mean age of patients with DCM was 50.7±11.5 years; the mean age of individuals in control group was 51.3±9.8 years. Risk factors detected at baseline in each group included age, sex, body mass index, smoking status, diabetes and blood pressure. Genomic DNA was extracted from leukocytes by a standard methodology and screened for rs10927875 polymorphism in intron of ZBTB17 gene using Real-time PCR method (Step One Applied Biosystems). The distribution of investigated genotypes for rs10927875 polymorphism in the group of patients with DCM was as follows: CC (89.74%), CT (10.26%), TT (0%), and the distribution in the control group: CC (92.31%), CT (5.13%), and TT (2.56%). Using the chi-square (χ2) test we compared genotype and allele frequencies between patients and controls. There was no difference in genotype or allele frequencies in ZBTB17 gene rs10927875 polymorphism between patients and control group (χ2=3.028, p=0.220; χ2=0.264, p=0.608). Our results represent an initial study, it can be considered as preliminary and first of its kind in Slovak population. Further studies of ZBTB17 gene polymorphisms of more numerous files and additional functional investigations are needed to fully understand the role of genetic associations. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=dilated%20cardiomyopathy" title="dilated cardiomyopathy">dilated cardiomyopathy</a>, <a href="https://publications.waset.org/abstracts/search?q=SNP%20polymorphism" title=" SNP polymorphism"> SNP polymorphism</a>, <a href="https://publications.waset.org/abstracts/search?q=ZBTB17%20gene" title=" ZBTB17 gene"> ZBTB17 gene</a>, <a href="https://publications.waset.org/abstracts/search?q=bioscience" title=" bioscience"> bioscience</a> </p> <a href="https://publications.waset.org/abstracts/1284/analysis-of-zbtb17-gene-rs10927875-polymorphism-in-relation-to-dilated-cardiomyopathy-in-slovak-population" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/1284.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">384</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7656</span> Electricity Consumption and Economic Growth: The Case of Mexico</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mario%20G%C3%B3mez">Mario Gómez</a>, <a href="https://publications.waset.org/abstracts/search?q=Jos%C3%A9%20Carlos%20Rodr%C3%ADguez"> José Carlos Rodríguez</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The causal relationship between energy consumption and economic growth has been an important issue in the economic literature. This paper studies the causal relationship between electricity consumption and economic growth in Mexico for the period of 1971-2011. In so doing, unit root tests and causality test are applied. The results show that the series are stationary in levels and that there is causality running from economic growth to energy consumption. The energy conservation policies have little or no impact on economic growth in México. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=causality" title="causality">causality</a>, <a href="https://publications.waset.org/abstracts/search?q=economic%20growth" title=" economic growth"> economic growth</a>, <a href="https://publications.waset.org/abstracts/search?q=energy%20consumption" title=" energy consumption"> energy consumption</a>, <a href="https://publications.waset.org/abstracts/search?q=Mexico" title=" Mexico"> Mexico</a> </p> <a href="https://publications.waset.org/abstracts/27583/electricity-consumption-and-economic-growth-the-case-of-mexico" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/27583.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">858</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7655</span> Mutation Analysis of the ATP7B Gene in 43 Vietnamese Wilson’s Disease Patients</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Huong%20M.%20T.%20Nguyen">Huong M. T. Nguyen</a>, <a href="https://publications.waset.org/abstracts/search?q=Hoa%20A.%20P.%20Nguyen"> Hoa A. P. Nguyen</a>, <a href="https://publications.waset.org/abstracts/search?q=Mai%20P.%20T.%20Nguyen"> Mai P. T. Nguyen</a>, <a href="https://publications.waset.org/abstracts/search?q=Ngoc%20D.%20Ngo"> Ngoc D. Ngo</a>, <a href="https://publications.waset.org/abstracts/search?q=Van%20T.%20Ta"> Van T. Ta</a>, <a href="https://publications.waset.org/abstracts/search?q=Hai%20T.%20Le"> Hai T. Le</a>, <a href="https://publications.waset.org/abstracts/search?q=Chi%20V.%20Phan"> Chi V. Phan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Wilson’s disease (WD) is an autosomal recessive disorder of the copper metabolism, which is caused by a mutation in the copper-transporting P-type ATPase (<em>ATP7B</em>). The mechanism of this disease is the failure of hepatic excretion of copper to bile, and leads to copper deposits in the liver and other organs. The <em>ATP7B</em> gene is located on the long arm of chromosome 13 (13q14.3). This study aimed to investigate the gene mutation in the Vietnamese patients with WD, and make a presymptomatic diagnosis for their familial members. Forty-three WD patients and their 65 siblings were identified as having <em>ATP7B</em> gene mutations. Genomic DNA was extracted from peripheral blood samples; 21 exons and exon-intron boundaries of the <em>ATP7B</em> gene were analyzed by direct sequencing. We recognized four mutations ([R723=; H724Tfs*34], V1042Cfs*79, D1027H, and IVS6+3A>G) in the sum of 20 detectable mutations, accounting for 87.2% of the total. Mutation S105* was determined to have a high rate (32.6%) in this study. The hotspot regions of <em>ATP7B</em> were found at exons 2, 16, and 8, and intron 14, in 39.6 %, 11.6 %, 9.3%, and 7 % of patients, respectively. Among nine homozygote/compound heterozygote siblings of the patients with WD, three individuals were determined as asymptomatic by screening mutations of the probands. They would begin treatment after diagnosis. In conclusion, 20 different mutations were detected in 43 WD patients. Of this number, four novel mutations were explored, including [R723=; H724Tfs*34], V1042Cfs*79, D1027H, and IVS6+3A>G. The mutation S105* is the most prevalent and has been considered as a biomarker that can be used in a rapid detection assay for diagnosis of WD patients. Exons 2, 8, and 16, and intron 14 should be screened initially for WD patients in Vietnam. Based on risk profile for WD, genetic testing for presymptomatic patients is also useful in diagnosis and treatment. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ATP7B%20gene" title="ATP7B gene">ATP7B gene</a>, <a href="https://publications.waset.org/abstracts/search?q=mutation%20detection" title=" mutation detection"> mutation detection</a>, <a href="https://publications.waset.org/abstracts/search?q=presymptomatic%20diagnosis" title=" presymptomatic diagnosis"> presymptomatic diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=Vietnamese%20Wilson%E2%80%99s%20disease" title=" Vietnamese Wilson’s disease"> Vietnamese Wilson’s disease</a> </p> <a href="https://publications.waset.org/abstracts/58250/mutation-analysis-of-the-atp7b-gene-in-43-vietnamese-wilsons-disease-patients" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/58250.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">380</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7654</span> PMEL Marker Identification of Dark and Light Feather Colours in Local Canary</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mudawamah%20Mudawamah">Mudawamah Mudawamah</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Z.%20Fadli"> Muhammad Z. Fadli</a>, <a href="https://publications.waset.org/abstracts/search?q=Gatot%20Ciptadi"> Gatot Ciptadi</a>, <a href="https://publications.waset.org/abstracts/search?q=Aulanni%E2%80%99am"> Aulanni’am</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Canary breeders have spread throughout Indonesian regions for the low-middle society and become an income source for them. The interesting phenomenon of the canary market is the feather colours become one of determining factor for the price. The advantages of this research were contributed to the molecular database as a base of selection and mating for the Indonesia canary breeder. The research method was experiment with the genome obtained from canary blood isolation. The genome did the PCR amplification with PMEL marker followed by sequencing. Canaries were used 24 heads of light and dark colour feathers. Research data analyses used BioEdit and Network 4.6.0.0 software. The results showed that all samples were amplification with PMEL gene with 500 bp fragment length. In base sequence of 40 was found Cytosine(C) in the light colour canaries, while the dark colour canaries was obtained Thymine (T) in same base sequence. Sequence results had 286-415 bp fragment and 10 haplotypes. The conclusions were the PMEL gene (gene of white pigment) was likely to be used PMEL gene to detect molecular genetic variation of dark and light colour feather. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=canary" title="canary">canary</a>, <a href="https://publications.waset.org/abstracts/search?q=haplotype" title=" haplotype"> haplotype</a>, <a href="https://publications.waset.org/abstracts/search?q=PMEL" title=" PMEL"> PMEL</a>, <a href="https://publications.waset.org/abstracts/search?q=sequence" title=" sequence"> sequence</a> </p> <a href="https://publications.waset.org/abstracts/39620/pmel-marker-identification-of-dark-and-light-feather-colours-in-local-canary" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/39620.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">237</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7653</span> Evaluating Gene-Gene Interaction among Nicotine Dependence Genes on the Risk of Oral Clefts</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mengying%20Wang">Mengying Wang</a>, <a href="https://publications.waset.org/abstracts/search?q=Dongjing%20Liu"> Dongjing Liu</a>, <a href="https://publications.waset.org/abstracts/search?q=Holger%20%20Schwender"> Holger Schwender</a>, <a href="https://publications.waset.org/abstracts/search?q=Ping%20Wang"> Ping Wang</a>, <a href="https://publications.waset.org/abstracts/search?q=Hongping%20Zhu"> Hongping Zhu</a>, <a href="https://publications.waset.org/abstracts/search?q=Tao%20Wu"> Tao Wu</a>, <a href="https://publications.waset.org/abstracts/search?q=Terri%20H%20Beaty"> Terri H Beaty</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Maternal smoking is a recognized risk factor for nonsyndromic cleft lip with or without cleft palate (NSCL/P). It has been reported that the effect of maternal smoking on oral clefts is mediated through genes that influence nicotine dependence. The polymorphisms of cholinergic receptor nicotinic alpha (CHRNA) and beta (CHRNB) subunits genes have previously shown strong associations with nicotine dependence. Here, we attempted to investigate whether the above genes are associated with clefting risk through testing for potential gene-gene (G×G) and gene-environment (G×E) interaction. Methods: We selected 120 markers in 14 genes associated with nicotine dependence to conduct transmission disequilibrium tests among 806 Chinese NSCL/P case-parent trios ascertained in an international consortium which conducted a genome-wide association study (GWAS) of oral clefts. We applied Cordell’s method using “TRIO” package in R to explore G×G as well as G×E interaction involving environmental tobacco smoke (ETS) based on conditional logistic regression model. Results: while no SNP showed significant association with NSCL/P after Bonferroni correction, we found signals for G×G interaction between 10 pairs of SNPs in CHRNA3, CHRNA5, and CHRNB4 (p<10-8), among which the most significant interaction was found between RS3743077 (CHRNA3) and RS11636753 (CHRNB4, p<8.2×10-12). Linkage disequilibrium (LD) analysis revealed only low level of LD between these markers. However, there were no significant results for G×ETS interaction. Conclusion: This study fails to detect association between nicotine dependence genes and NSCL/P, but illustrates the importance of taking into account potential G×G interaction for genetic association analysis in NSCL/P. This study also suggests nicotine dependence genes should be considered as important candidate genes for NSCL/P in future studies. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gene-Gene%20Interaction" title="Gene-Gene Interaction">Gene-Gene Interaction</a>, <a href="https://publications.waset.org/abstracts/search?q=Maternal%20Smoking" title=" Maternal Smoking"> Maternal Smoking</a>, <a href="https://publications.waset.org/abstracts/search?q=Nicotine%20Dependence" title=" Nicotine Dependence"> Nicotine Dependence</a>, <a href="https://publications.waset.org/abstracts/search?q=Non-Syndromic%20Cleft%20Lip%20with%20or%20without%20Cleft%20Palate" title=" Non-Syndromic Cleft Lip with or without Cleft Palate"> Non-Syndromic Cleft Lip with or without Cleft Palate</a> </p> <a href="https://publications.waset.org/abstracts/66238/evaluating-gene-gene-interaction-among-nicotine-dependence-genes-on-the-risk-of-oral-clefts" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/66238.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">337</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7652</span> The Genetic Basis of the Lack of Impulse Control: What is Provided for the Criminal Law?</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Amir%20Bastani">Amir Bastani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The result of the research in the field of human behavioural genetics demonstrates a genetic contribution of behavioural differences in aggression, violence, drug and substance abuse, antisocial personality disorder and other related traits. As the field of human behavioural genetics progresses and achieves credibility, the criminal accused continue to use its types of evidence into the criminal law. One of the most important genetic factors which controls certain neurotransmitters like dopamine and serotonin is the Monoamine Oxidase Acid A (MAOA) gene, known as the 'warrior gene'. The high-profile study by Caspi and colleagues in 2002 showed that the combination between one type of variation of the MAOA gene and childhood maltreatment noticeably predisposes a person to antisocial behaviour. Moreover, further scientific research shows that individuals with the MAOA gene have to some degree difficulties in controlling their impulses. Based on the evidence of MAOA, some criminal accused claimed difficulties in self-control. In the first case – the famous case of Mobley – the court rejected the MAOA evidence on the ground of the lack of scientific support. In contrast, in other cases after the Mobley trial, courts accepted the evidence of MAOA. In this paper, the issue of lack of impulse control produced by the MAOA gene and cases which relied on the MAOA evidence and successfully being accepted will be reviewed in detail. Finally, the anticipation of the paper for the future use of the MAOA evidence in criminal cases will be presented. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=genetic%20defence" title="genetic defence">genetic defence</a>, <a href="https://publications.waset.org/abstracts/search?q=criminal%20responsibility" title=" criminal responsibility"> criminal responsibility</a>, <a href="https://publications.waset.org/abstracts/search?q=MAOA" title=" MAOA"> MAOA</a>, <a href="https://publications.waset.org/abstracts/search?q=self-control" title=" self-control"> self-control</a> </p> <a href="https://publications.waset.org/abstracts/20503/the-genetic-basis-of-the-lack-of-impulse-control-what-is-provided-for-the-criminal-law" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/20503.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">472</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7651</span> Mutational and Evolutionary Analysis of Interleukin-2 Gene in Four Pakistani Goat Breeds</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tanveer%20Hussain">Tanveer Hussain</a>, <a href="https://publications.waset.org/abstracts/search?q=Misbah%20Hussain"> Misbah Hussain</a>, <a href="https://publications.waset.org/abstracts/search?q=Masroor%20Ellahi%20Babar"> Masroor Ellahi Babar</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Traiq%20Pervez"> Muhammad Traiq Pervez</a>, <a href="https://publications.waset.org/abstracts/search?q=Fiaz%20Hussain"> Fiaz Hussain</a>, <a href="https://publications.waset.org/abstracts/search?q=Sana%20Zahoor"> Sana Zahoor</a>, <a href="https://publications.waset.org/abstracts/search?q=Rashid%20Saif"> Rashid Saif</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Interleukin 2 (IL-2) is a cytokine which is produced by activated T cells, play important role in immune response against antigen. It act in both autocrine and paracrine manner. It can stimulate B cells and various other phagocytic cells like monocytes, lymphokine-activated killer cells and natural killer cells. Acting in autocrine fashion, IL-2 protein plays a crucial role in proliferation of T cells. IL-2 triggers the release of pro and anti- inflammatory cytokines by activating several pathways. In present study, exon 1 of IL-2 gene of four local Pakistani breeds (Dera Din Panah, Beetal, Nachi and Kamori) from two provinces was amplified by using reported Ovine IL-2 primers, yielding PCR product of 501 bp. The sequencing of all samples was done to identify the polymorphisms in amplified region of IL-2 gene. Analysis of sequencing data resulted in identification of one novel nucleotide substitution (T→A) in amplified non-coding region of IL-2 gene. Comparison of IL-2 gene sequence of all four breeds with other goat breeds showed high similarity in sequence. While phylogenetic analysis of our local breeds with other mammals showed that IL-2 is a variable gene which has undergone many substitutions. This high substitution rate can be due to the decreased or increased changed selective pressure. These rapid changes can also lead to the change in function of immune system. This pioneering study of Pakistani goat breeds urge for further studies on immune system of each targeted breed for fully understanding the functional role of IL-2 in goat immunity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=interleukin%202" title="interleukin 2">interleukin 2</a>, <a href="https://publications.waset.org/abstracts/search?q=mutational%20analysis" title=" mutational analysis"> mutational analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=phylogeny" title=" phylogeny"> phylogeny</a>, <a href="https://publications.waset.org/abstracts/search?q=goat%20breeds" title=" goat breeds"> goat breeds</a>, <a href="https://publications.waset.org/abstracts/search?q=Pakistan" title=" Pakistan"> Pakistan</a> </p> <a href="https://publications.waset.org/abstracts/26888/mutational-and-evolutionary-analysis-of-interleukin-2-gene-in-four-pakistani-goat-breeds" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/26888.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">610</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7650</span> Loss of Function of Only One of Two CPR5 Paralogs Causes Resistance Against Rice Yellow Mottle Virus</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yugander%20Arra">Yugander Arra</a>, <a href="https://publications.waset.org/abstracts/search?q=Florence%20Auguy"> Florence Auguy</a>, <a href="https://publications.waset.org/abstracts/search?q=Melissa%20Stiebner"> Melissa Stiebner</a>, <a href="https://publications.waset.org/abstracts/search?q=Sophie%20Ch%C3%A9ron"> Sophie Chéron</a>, <a href="https://publications.waset.org/abstracts/search?q=Michael%20M.%20Wudick"> Michael M. Wudick</a>, <a href="https://publications.waset.org/abstracts/search?q=Van%20Schepler-Luu"> Van Schepler-Luu</a>, <a href="https://publications.waset.org/abstracts/search?q=S%C3%A9bastien%20Cunnac"> Sébastien Cunnac</a>, <a href="https://publications.waset.org/abstracts/search?q=Wolf%20B.%20Frommer"> Wolf B. Frommer</a>, <a href="https://publications.waset.org/abstracts/search?q=Laurence%20Albar"> Laurence Albar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Rice yellow mottle virus (RYMV) is one of the most important diseases affecting rice in Africa. The most promising strategy to reduce yield losses is the use of highly resistant varieties. The resistance gene RYMV2 is homolog of the Arabidopsis constitutive expression of pathogenesis related protein-5 (AtCPR5) nucleoporin gene. Resistance alleles are originating from African cultivated rice Oryza glaberrima, rarely cultivated, and are characterized by frameshifts or early stop codons, leading to a non-functional or truncated protein. Rice possesses two paralogs of CPR5 and function of these genes are unclear. Here, we evaluated the role of the two rice candidate nucleoporin paralogs OsCPR5.1 (pathogenesis-related gene 5; RYMV2) and OsCPR5.2 by CRISPR/Cas9 genome editing. Despite striking sequence and structural similarity, only loss-of-function of OsCPR5.1 led to full resistance, while loss-of-function oscpr5.2 mutants remained susceptible. Short N-terminal deletions in OsCPR5.1 also did not lead to resistance. In contrast to Atcpr5 mutants, neither OsCPR5.1 nor OsCPR5.2 knock out mutants showed substantial growth defects. Taken together, the candidate nucleoporin OsCPR5.1, but not its close homolog OsCPR5.2, plays a specific role for the susceptibility to RYMV, possibly by impairing the import of viral RNA or protein into the nucleus. Whereas gene introgression from O. glaberrima to high yielding O. sativa varieties is impaired by strong sterility barriers and the negative impact of linkage drag, genome editing of OsCPR5.1, while maintaining OsCPR5.2 activity, thus provides a promising strategy to generate O. sativa elite lines that are resistant to RYMV. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=CRISPR%20Cas9" title="CRISPR Cas9">CRISPR Cas9</a>, <a href="https://publications.waset.org/abstracts/search?q=genome%20editing" title=" genome editing"> genome editing</a>, <a href="https://publications.waset.org/abstracts/search?q=knock%20out%20mutant" title=" knock out mutant"> knock out mutant</a>, <a href="https://publications.waset.org/abstracts/search?q=recessive%20resistance" title=" recessive resistance"> recessive resistance</a>, <a href="https://publications.waset.org/abstracts/search?q=rice%20yellow%20mottle%20virus" title=" rice yellow mottle virus"> rice yellow mottle virus</a> </p> <a href="https://publications.waset.org/abstracts/155739/loss-of-function-of-only-one-of-two-cpr5-paralogs-causes-resistance-against-rice-yellow-mottle-virus" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/155739.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">118</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7649</span> The Pharmacogenetics of Type 1 Cannabinoid Receptor (CB1) Gene Associated with Adverse Drug Reactions in Thai Patients</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kittitara%20Chunlakittiphan">Kittitara Chunlakittiphan</a>, <a href="https://publications.waset.org/abstracts/search?q=Patompong%20Satapornpong"> Patompong Satapornpong</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: The variation of genetics affects how our body responds to pharmaceuticals elucidates the correlation between long-term use of medical cannabis and adverse drug reactions (ADRs). Medical cannabis is regarded as the treatment for chronic pain, cancer pain, acute pain, psychological disorders, multiple sclerosis and migraine management. However, previous studies have shown that delta-9-Tetrahydrocannabinol (THC), an ingredient found in cannabis, was the cause of ADRs in CB1 receptors found in humans. Previous research suggests that distributions of the cannabinoid type 1 (CB1) receptor gene and pharmacogenetic markers, which vary amongst different populations, might affect incidences of ADRs. Although there is an evident need to investigate the level of the CB1 receptor gene (rs806365), studies on the distribution of CB1-pharmacogenetics markers in Thai patients are limited. Objective: Therefore, the aim of this study is to investigate the distribution of the rs806365 polymorphism in Thai patients who have been treated with medical cannabis. Materials and Methods: We enrolled 31 Thai patients with THC-induced ADRs and 34 THC-tolerant controls to take part in this study. All patients with THC-induced ADRs were accessed through a review of medical records by physicians. EDTA blood of 3ml was collected to obtain the CNR1 gene (rs806365) and genotyping of this gene was conducted using the real-time PCR ViiA7 (ABI, Foster City, CA, USA) following the manufacturer’s instruction. Results: The sample consisted of 65 patients (40/61.54%) were females and (25/38.46%) were males, with an age range of 19-87 years, who have been treated with medical cannabis. In this study, the most common THC-induced ADRs were dry mouth and/or dry throat, tachycardia, nausea, and arrhythmia. Across the whole sample, we found that 52.31% of Thai patients carried a heterozygous variant (rs806365, CT allele). Moreover, the number of rs806365 (CC, homozygous variant) carriers totaled seventeen people (26.15%) amongst the subjects of Thai patients treated with medical cannabis. Furthermore, 17 out of 22 patients (77.27%) who experienced severe ADRs: Tachycardia and/or arrhythmia, carried an abnormal rs806365 gene (CT and CC alleles). Conclusions: The results propose that the rs806365 gene is widely distributed amongst the Thai population and there is a link between this gene and vulnerability to developing THC-induced ADRs after being treated with medical cannabis. Therefore, it is necessary to screen for the rs806365 gene before using medical cannabis to treat a patient. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=rs806365" title="rs806365">rs806365</a>, <a href="https://publications.waset.org/abstracts/search?q=THC-induced%20adverse%20drug%20reactions" title=" THC-induced adverse drug reactions"> THC-induced adverse drug reactions</a>, <a href="https://publications.waset.org/abstracts/search?q=CB1%20receptor" title=" CB1 receptor"> CB1 receptor</a>, <a href="https://publications.waset.org/abstracts/search?q=Thai%20population" title=" Thai population"> Thai population</a> </p> <a href="https://publications.waset.org/abstracts/148193/the-pharmacogenetics-of-type-1-cannabinoid-receptor-cb1-gene-associated-with-adverse-drug-reactions-in-thai-patients" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/148193.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">101</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7648</span> Screening and Evaluation of Plant Growth Promoting Rhizobacteria of Wheat/Faba Bean for Increasing Productivity and Yield</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yasir%20Arafat">Yasir Arafat</a>, <a href="https://publications.waset.org/abstracts/search?q=Asma%20Shah"> Asma Shah</a>, <a href="https://publications.waset.org/abstracts/search?q=Hua%20Shao"> Hua Shao</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background and Aims: Legume/cereal intercropping is used worldwide for enhancement in biomass and yield of cereal crops. However, because of intercropping, the belowground biological and chemical interactions and their effect on physiological parameters and yield of crops are limited. Methods: Wheat faba bean (WF) intercropping was designed to understand the underlying changes in the soil's chemical environment, soil microbial communities, and effect on growth and yield parameters. Experimental plots were established as having no root partition (NRP), semi-root partition (SRP), complete root partition (CRP), and their sole cropping (CK). Low molecular weight organic acids (LMWOAs) were determined by GC-MS, and high throughput sequencing of the 16S rRNA gene was carried out to screen microbial structure and composition in different root partitions of the WF intercropping system. Results: We show that intercropping induced a shift in the relative abundance of some genera of plant growth promoting rhizobacteria (PGPR) such as Allorhizobium, Neorhizobium, Pararhizobium, and Rhizobium species and resulted in better growth and yield performance of wheat. Moreover, as the plant's distance of wheat from faba beans decreased, the diversity of microbes increased, and a positive effect was observed on physiological traits and crop yield. Furthermore, an abundance and positive correlations of palmitic acid, arachidic acid, stearic acid, and 9-Octadecenoic with PGPR were recorded in the root zone of WF intercropping, which can play an important role in this facilitative mechanism of enhancing growth and yield of cereals. Conclusion: The two treatments clearly affected soil microbial and chemical composition, which can be reflected in growth and yield enhancement. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=intercropping" title="intercropping">intercropping</a>, <a href="https://publications.waset.org/abstracts/search?q=microbial%20community" title=" microbial community"> microbial community</a>, <a href="https://publications.waset.org/abstracts/search?q=LMWOAs" title=" LMWOAs"> LMWOAs</a>, <a href="https://publications.waset.org/abstracts/search?q=PGPR" title=" PGPR"> PGPR</a>, <a href="https://publications.waset.org/abstracts/search?q=soil%20chemical%20environment" title=" soil chemical environment"> soil chemical environment</a> </p> <a href="https://publications.waset.org/abstracts/179034/screening-and-evaluation-of-plant-growth-promoting-rhizobacteria-of-wheatfaba-bean-for-increasing-productivity-and-yield" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/179034.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">84</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7647</span> Trans-Sphenoidal Approach to Pituitary Tumors: Analysis of 568 Cases Over a Decade</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sandeep%20Mohindra">Sandeep Mohindra</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Since 2013, the authors have operated on 568 cases of pituitary tumors through the trans-sphenoidal route, using the binostril approach. The distribution included 486 cases of non-functioning pituitary tumors, 24 cases of Growth hormone(GH) secreting tumors(acromegaly), and 28 cases of adrenocorticotrophic(ACTH) secreting tumors(Cushing's Disease). The authors utilized neuro-navigation for 18 cases, and all belonged to the functional tumor category. Complications included ICA injury in 2 cases, fatal meningitis in 5 cases, while CSF leak required repair in 28 cases. Satisfactory excision was noted in 512 cases, while recurrence/residual required repeat surgery in 32 cases. Authors conclude that trans sphenoidal route remains the best and optimal way of managing sellar tumors, especially pituitary adenomas. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=pituitary" title="pituitary">pituitary</a>, <a href="https://publications.waset.org/abstracts/search?q=adenoma" title=" adenoma"> adenoma</a>, <a href="https://publications.waset.org/abstracts/search?q=trans-sphenoidal" title=" trans-sphenoidal"> trans-sphenoidal</a>, <a href="https://publications.waset.org/abstracts/search?q=endonasal" title=" endonasal"> endonasal</a>, <a href="https://publications.waset.org/abstracts/search?q=neuronavigation" title=" neuronavigation"> neuronavigation</a> </p> <a href="https://publications.waset.org/abstracts/185181/trans-sphenoidal-approach-to-pituitary-tumors-analysis-of-568-cases-over-a-decade" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/185181.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">63</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7646</span> The Differences of Vascular Endothelial Growth Factor Levels in Serum to Determine Follicular Adenoma and Follicular Carcinoma of Thyroid</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tery%20Nehemia%20Nugraha%20Joseph">Tery Nehemia Nugraha Joseph</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20D.%20P.%20Wisnubroto"> J. D. P. Wisnubroto</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Thyroid cancer is a healthcare problem with high morbidity and mortality. Follicular adenoma and follicular carcinoma are thyroid tumors from the thyroid follicular cells differentiation with a microfollicular pattern that consists of follicular cuboidal cells. vascular endothelial growth factor (VEGF) is a potent and powerful mitogen for endothelial cells and increases vascular permeability. Therefore, due to an increase in thyroid-stimulating hormone (TSH), VEGF production is activated in the thyroid that leads to the end of mitogenic TSH stimulation and initiation of angiogenesis. The differences in VEGF levels in the follicular carcinoma of thyroid tissue with follicular adenoma thyroid can be used as a basis in differentiating the two types of neoplasms. This study aims to analyze VEGF in the serum so that it can be used to differentiate the types of thyroid carcinoma before surgery. This study uses a cross-sectional research design. Samples were carried out by taking serum samples, and the VEGF levels were calculated. Data were analyzed using the Mann-Whitney test. The results found a significant difference between VEGF levels in the follicular carcinoma thyroid group and VEGF levels in the follicular adenoma thyroid group with a value of p = 0.007 (p < 0.05). The results obtained are 560,427 ± 160,506 ng/mL in the type of follicular carcinoma thyroid and 320.943 ± 134.573 ng/mL in the type of follicular adenoma thyroid. VEGF levels between follicular adenoma and follicular carcinoma are different. VEGF levels are higher in follicular carcinoma thyroid than follicular adenoma thyroid. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=follicular%20adenoma%20thyroid" title="follicular adenoma thyroid">follicular adenoma thyroid</a>, <a href="https://publications.waset.org/abstracts/search?q=follicular%20carcinoma%20thyroid" title=" follicular carcinoma thyroid"> follicular carcinoma thyroid</a>, <a href="https://publications.waset.org/abstracts/search?q=thyroid" title=" thyroid"> thyroid</a>, <a href="https://publications.waset.org/abstracts/search?q=VEGF" title=" VEGF"> VEGF</a> </p> <a href="https://publications.waset.org/abstracts/136980/the-differences-of-vascular-endothelial-growth-factor-levels-in-serum-to-determine-follicular-adenoma-and-follicular-carcinoma-of-thyroid" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/136980.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">143</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7645</span> Lack of Association between IL-10 Promoter Gene Polymorphisms and Tuberculosis Susceptibility in Thai Population</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Manaphol%20Kulpraneet">Manaphol Kulpraneet</a>, <a href="https://publications.waset.org/abstracts/search?q=Anirut%20Limtrakul"> Anirut Limtrakul</a>, <a href="https://publications.waset.org/abstracts/search?q=Surangrat%20Srisurapanon"> Surangrat Srisurapanon</a>, <a href="https://publications.waset.org/abstracts/search?q=Piyatida%20Tangteerawatana"> Piyatida Tangteerawatana</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Tuberculosis (TB) remains a global health care disease world-wide. Control of the global TB epidemic has been impaired by the lack of an effective vaccine, by the emergence of drug resistant forms of Mycobacterium tuberculosis and by lack of sensitive and rapid diagnostics. Cytokines play a major role in defense against M. tuberculosis infection. Polymorphisms in the genes encoding various cytokines have been associated with tuberculosis susceptibility. Polymorphisms of the regulatory cytokine gene, the interleukin (IL)-10 is associated with the risk of tuberculosis (TB) in different populations. However, IL-10 gene polymorphism and susceptibility to TB in Thai is still unknown. The purpose of this study was to evaluate whether the common IL-10 promoter gene polymorphisms are associated with TB in Thai population. Forty eight patients with newly diagnosed pulmonary tuberculosis were studied. DNA samples were extracted from leukocytes and used to investigate -1087A/G, -819C/T, -252C/A (rs1800896, rs1800871, rs1800872) in IL-10 gene using restriction fragment length polymorphism (PCR-RFLP) methods. In this study, the genotype and allele frequencies of IL-10-1087A/G, -819C/T, -252C/A polymorphism did not significantly different between TB patients and healthy controls ((genotype: p=0.38, p=0.92, p=1; allele: p=0.57, p=0.77, p=0.89, respectively). The lack of association between common IL-10 promoter polymorphisms and TB susceptibility in this study may provide clue for better understanding of IL-10-1087A/G, -819C/T, -252C/A polymorphism and TB susceptibility in Thai population, which might facilitate the rationale design of vaccines. However, further studies in large scales population are required for confirmation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=IL-10" title="IL-10">IL-10</a>, <a href="https://publications.waset.org/abstracts/search?q=cytokines" title=" cytokines"> cytokines</a>, <a href="https://publications.waset.org/abstracts/search?q=single%20nucleotide%20polymorphism%20%28SNP%29" title=" single nucleotide polymorphism (SNP)"> single nucleotide polymorphism (SNP)</a>, <a href="https://publications.waset.org/abstracts/search?q=tuberculosis" title=" tuberculosis"> tuberculosis</a> </p> <a href="https://publications.waset.org/abstracts/40459/lack-of-association-between-il-10-promoter-gene-polymorphisms-and-tuberculosis-susceptibility-in-thai-population" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/40459.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">333</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7644</span> Recovery of Helicobacter Pylori from Stagnant and Moving Water Biofilms</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Maryam%20Zafar">Maryam Zafar</a>, <a href="https://publications.waset.org/abstracts/search?q=Sajida%20Rasheed"> Sajida Rasheed</a>, <a href="https://publications.waset.org/abstracts/search?q=Imran%20Hashmi"> Imran Hashmi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Water as an environmental reservoir is reported to act as a habitat and transmission route to microaerophilic bacteria such as Heliobacter pylori. It has been studied that in biofilms are the predominant dwellings for the bacteria to grow in water and protective reservoir for numerous pathogens by protecting them against harsh conditions, such as shear stress, low carbon concentration and less than optimal temperature. In this study, influence of these and many other parameters was studied on H. pylori in stagnant and moving water biofilms both in surface and underground aquatic reservoirs. H. pylori were recovered from pipe of different materials such as Polyvinyl Chloride, Polypropylene and Galvanized iron pipe cross sections from an urban water distribution network. Biofilm swabbed from inner cross section was examined by molecular biology methods coupled with gene sequencing and H. pylori 16S rRNA peptide nucleic acid probe showing positive results for H. pylori presence. Studies showed that pipe material affect growth of biofilm which in turn provide additional survival mechanism for pathogens like H. pylori causing public health concerns. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biofilm" title="biofilm">biofilm</a>, <a href="https://publications.waset.org/abstracts/search?q=gene%20sequencing" title=" gene sequencing"> gene sequencing</a>, <a href="https://publications.waset.org/abstracts/search?q=heliobacter%20pylori" title=" heliobacter pylori"> heliobacter pylori</a>, <a href="https://publications.waset.org/abstracts/search?q=pipe%20materials" title=" pipe materials"> pipe materials</a> </p> <a href="https://publications.waset.org/abstracts/37768/recovery-of-helicobacter-pylori-from-stagnant-and-moving-water-biofilms" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/37768.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">359</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7643</span> Genetic Association of SIX6 Gene with Pathogenesis of Glaucoma</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Riffat%20Iqbal">Riffat Iqbal</a>, <a href="https://publications.waset.org/abstracts/search?q=Sidra%20Ihsan"> Sidra Ihsan</a>, <a href="https://publications.waset.org/abstracts/search?q=Andleeb%20Batool"> Andleeb Batool</a>, <a href="https://publications.waset.org/abstracts/search?q=Maryam%20Mukhtar"> Maryam Mukhtar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Glaucoma is a gathering of optic neuropathies described by dynamic degeneration of retinal ganglionic cells. It is clinically and innately heterogenous illness containing a couple of particular forms each with various causes and severities. Primary open-angle glaucoma (POAG) is the most generally perceived kind of glaucoma. This study investigated the genetic association of single nucleotide polymorphisms (SNPs; rs10483727 and rs33912345) at the SIX1/SIX6 locus with primary open-angle glaucoma (POAG) in the Pakistani population. The SIX6 gene plays an important role in ocular development and has been associated with morphology of the optic nerve. A total of 100 patients clinically diagnosed with glaucoma and 100 control individuals of age over 40 were enrolled in the study. Genomic DNA was extracted by organic extraction method. The SNP genotyping was done by (i) PCR based restriction fragment length polymorphism (RFLP) and sequencing method. Significant genetic associations were observed for rs10483727 (risk allele T) and rs33912345 (risk allele C) with POAG. Hence, it was concluded that Six6 gene is genetically associated with pathogenesis of Glaucoma in Pakistan. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=genotyping" title="genotyping">genotyping</a>, <a href="https://publications.waset.org/abstracts/search?q=Pakistani%20population" title=" Pakistani population"> Pakistani population</a>, <a href="https://publications.waset.org/abstracts/search?q=primary%20open-angle%20glaucoma" title=" primary open-angle glaucoma"> primary open-angle glaucoma</a>, <a href="https://publications.waset.org/abstracts/search?q=SIX6%20gene" title=" SIX6 gene"> SIX6 gene</a> </p> <a href="https://publications.waset.org/abstracts/102183/genetic-association-of-six6-gene-with-pathogenesis-of-glaucoma" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/102183.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">184</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7642</span> An Attenuated Quadruple Gene Mutant of Mycobacterium tuberculosis Imparts Protection against Tuberculosis in Guinea Pigs</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shubhita%20Mathur">Shubhita Mathur</a>, <a href="https://publications.waset.org/abstracts/search?q=Ritika%20Kar%20Bahal"> Ritika Kar Bahal</a>, <a href="https://publications.waset.org/abstracts/search?q=Priyanka%20Chauhan"> Priyanka Chauhan</a>, <a href="https://publications.waset.org/abstracts/search?q=Anil%20K.%20Tyagi"> Anil K. Tyagi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Mycobacterium tuberculosis, the causative agent of human tuberculosis, is a major cause of mortality. Bacillus Calmette-Guérin (BCG), the only licensed vaccine available for protection against tuberculosis confers highly variable protection ranging from 0%-80%. Thus, novel vaccine strains need to be evaluated for their potential as a vaccine against tuberculosis. We had previously constructed a triple gene mutant of M. tuberculosis (MtbΔmms), having deletions in genes encoding for phosphatases mptpA, mptpB, and sapM that are involved in host-pathogen interaction. Though vaccination with Mtb∆mms strain induced protection in the lungs of guinea pigs, the mutant strain was not able to control the hematogenous spread of the challenge strain to the spleens. Additionally, inoculation with Mtb∆mms resulted in some pathological damage to the spleens in the early phase of infection. In order to overcome the pathology caused by MtbΔmms in the spleens of guinea pigs and also to control the dissemination of the challenge strain, MtbΔmms was genetically modified by disrupting bioA gene to generate MtbΔmmsb strain. Further, in vivo attenuation of MtbΔmmsb was evaluated, and its protective efficacy was assessed against virulent M. tuberculosis challenge in guinea pigs. Our study demonstrates that Mtb∆mmsb mutant was highly attenuated for growth and virulence in guinea pigs. Vaccination with Mtb∆mmsb mutant generated significant protection in comparison to sham-immunized animals at 4 and 12 weeks post-infection in lungs and spleens of the infected animals. Our findings provide evidence that deletion of genes involved in signal transduction and biotin biosynthesis severely attenuates the pathogen and the single immunization with the auxotroph was able to provide significant protection as compared to sham-immunized animals. The protection imparted by Mtb∆mmsb fell short in comparison to the protection observed in BCG-immunized animals. This study nevertheless indicates the importance of attenuated multiple gene deletion mutants of M. tuberculosis in generating protection against tuberculosis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mycobacterium%20tuberculosis" title="Mycobacterium tuberculosis">Mycobacterium tuberculosis</a>, <a href="https://publications.waset.org/abstracts/search?q=BCG" title=" BCG"> BCG</a>, <a href="https://publications.waset.org/abstracts/search?q=Mtb%CE%94mmsb" title=" MtbΔmmsb"> MtbΔmmsb</a>, <a href="https://publications.waset.org/abstracts/search?q=bioA" title=" bioA"> bioA</a>, <a href="https://publications.waset.org/abstracts/search?q=guinea%20pigs" title=" guinea pigs"> guinea pigs</a> </p> <a href="https://publications.waset.org/abstracts/109444/an-attenuated-quadruple-gene-mutant-of-mycobacterium-tuberculosis-imparts-protection-against-tuberculosis-in-guinea-pigs" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/109444.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">140</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7641</span> Potential Growth of Tomato Plants in Induced Saline Soil with Rhizobacteria (PGPR)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Arfan%20Ali">Arfan Ali</a>, <a href="https://publications.waset.org/abstracts/search?q=Idrees%20Ahmad%20Nasir"> Idrees Ahmad Nasir</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The critical evaluation of tolerance in tomato plants against the induced saline soil were assessed by transcript analysis of genes coding for products potentially involved in stress tolerance. A reverse transcriptase PCR experiment was performed with Hsp90-1, MT2, and GR1like protein genes using RNA isolated from different tissues of tomato plants. Four strains of Bacillus magisterium were inoculated with 100 Mm & 200 Mm concentrations of salt. Eleven treatments each ten replica pots were installed in green house experiment and the parameters taken into account were morphological (length, weight, number of leaves, leaf surface area), chemical (anthocyanin, chlorophyll-a, chlorophyll-b, carotenoids) and biological (gene expression). Results bare a response i.e. highest response of MT2 like gene was at 24 hpi and the highest levels of GR1 like protein transcript accumulation were detected at 36 hpi. The chemical and morphological parameters at diverse salt concentrations bequeath superlative response amongst strains which candidly flank on Zm7 and Zm4. Therefore, Bacillus magisterium Zm7 strains and somehow Zm4 strain can be used in saline condition to make plants tolerant. The overall performance of strains Zm7, Zm6, and Zm4 was found better for all studied traits under salt stress conditions. Significant correlations among traits root length, shoot length, number of leaves, leaf surface area, carotenoids, anthocyanin, chlorophyll-a and chlorophyll-b were found and suggested that the salt tolerance in tomato may be improved through the use of PGPR strains. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bacillus%20magisterium" title="Bacillus magisterium">Bacillus magisterium</a>, <a href="https://publications.waset.org/abstracts/search?q=gene%20expression%20glutathione%20reductase" title=" gene expression glutathione reductase"> gene expression glutathione reductase</a>, <a href="https://publications.waset.org/abstracts/search?q=metallothionein" title=" metallothionein"> metallothionein</a>, <a href="https://publications.waset.org/abstracts/search?q=PGPR" title=" PGPR"> PGPR</a>, <a href="https://publications.waset.org/abstracts/search?q=Rhizobacteria" title=" Rhizobacteria"> Rhizobacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=saline" title=" saline "> saline </a> </p> <a href="https://publications.waset.org/abstracts/25192/potential-growth-of-tomato-plants-in-induced-saline-soil-with-rhizobacteria-pgpr" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/25192.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">434</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7640</span> Computational Model for Predicting Effective siRNA Sequences Using Whole Stacking Energy (ΔG) for Gene Silencing</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Reena%20Murali">Reena Murali</a>, <a href="https://publications.waset.org/abstracts/search?q=David%20Peter%20S."> David Peter S.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The small interfering RNA (siRNA) alters the regulatory role of mRNA during gene expression by translational inhibition. Recent studies shows that up regulation of mRNA cause serious diseases like Cancer. So designing effective siRNA with good knockdown effects play an important role in gene silencing. Various siRNA design tools had been developed earlier. In this work, we are trying to analyze the existing good scoring second generation siRNA predicting tools and to optimize the efficiency of siRNA prediction by designing a computational model using Artificial Neural Network and whole stacking energy (ΔG), which may help in gene silencing and drug design in cancer therapy. Our model is trained and tested against a large data set of siRNA sequences. Validation of our results is done by finding correlation coefficient of experimental versus observed inhibition efficacy of siRNA. We achieved a correlation coefficient of 0.727 in our previous computational model and we could improve the correlation coefficient up to 0.753 when the threshold of whole tacking energy is greater than or equal to -32.5 kcal/mol. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=artificial%20neural%20network" title="artificial neural network">artificial neural network</a>, <a href="https://publications.waset.org/abstracts/search?q=double%20stranded%20RNA" title=" double stranded RNA"> double stranded RNA</a>, <a href="https://publications.waset.org/abstracts/search?q=RNA%20interference" title=" RNA interference"> RNA interference</a>, <a href="https://publications.waset.org/abstracts/search?q=short%20interfering%20RNA" title=" short interfering RNA"> short interfering RNA</a> </p> <a href="https://publications.waset.org/abstracts/16841/computational-model-for-predicting-effective-sirna-sequences-using-whole-stacking-energy-dg-for-gene-silencing" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/16841.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">526</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7639</span> The Stem Cell Transcription Co-factor Znf521 Sustains Mll-af9 Fusion Protein In Acute Myeloid Leukemias By Altering The Gene Expression Landscape</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Emanuela%20Chiarella">Emanuela Chiarella</a>, <a href="https://publications.waset.org/abstracts/search?q=Annamaria%20Aloisio"> Annamaria Aloisio</a>, <a href="https://publications.waset.org/abstracts/search?q=Nistic%C3%B2%20Clelia"> Nisticò Clelia</a>, <a href="https://publications.waset.org/abstracts/search?q=Maria%20Mesuraca"> Maria Mesuraca</a> </p> <p class="card-text"><strong>Abstract:</strong></p> ZNF521 is a stem cell-associated transcription co-factor, that plays a crucial role in the homeostatic regulation of the stem cell compartment in the hematopoietic, osteo-adipogenic, and neural system. In normal hematopoiesis, primary human CD34+ hematopoietic stem cells display typically a high expression of ZNF521, while its mRNA levels rapidly decrease when these progenitors progress towards erythroid, granulocytic, or B-lymphoid differentiation. However, most acute myeloid leukemias (AMLs) and leukemia-initiating cells keep high ZNF521 expression. In particular, AMLs are often characterized by chromosomal translocations involving the Mixed Lineage Leukemia (MLL) gene, which MLL gene includes a variety of fusion oncogenes arisen from genes normally required during hematopoietic development; once they are fused, they promote epigenetic and transcription factor dysregulation. The chromosomal translocation t(9;11)(p21-22;q23), fusing the MLL gene with AF9 gene, results in a monocytic immune phenotype with an aggressive course, frequent relapses, and a short survival time. To better understand the dysfunctional transcriptional networks related to genetic aberrations, AML gene expression profile datasets were queried for ZNF521 expression and its correlations with specific gene rearrangements and mutations. The results showed that ZNF521 mRNA levels are associated with specific genetic aberrations: the highest expression levels were observed in AMLs involving t(11q23) MLL rearrangements in two distinct datasets (MILE and den Boer); elevated ZNF521 mRNA expression levels were also revealed in AMLs with t(7;12) or with internal rearrangements of chromosome 16. On the contrary, relatively low ZNF521 expression levels seemed to be associated with the t(8;21) translocation, that in turn is correlated with the AML1-ETO fusion gene or the t(15;17) translocation and in AMLs with FLT3-ITD, NPM1, or CEBPα double mutations. Invitro, we found that the enforced co-expression of ZNF521 in cord blood-derived CD34+ cells induced a significant proliferative advantage, improving MLL-AF9 effects on the induction of proliferation and the expansion of leukemic progenitor cells. Transcriptome profiling of CD34+ cells transduced with either MLL-AF9, ZNF521, or a combination of the two transgenes highlighted specific sets of up- or down-regulated genes that are involved in the leukemic phenotype, including those encoding transcription factors, epigenetic modulators, and cell cycle regulators as well as those engaged in the transport or uptake of nutrients. These data enhance the functional cooperation between ZNF521 and MA9, resulting in the development, maintenance, and clonal expansion of leukemic cells. Finally, silencing of ZNF521 in MLL-AF9-transformed primary CD34+ cells inhibited their proliferation and led to their extinction, as well as ZNF521 silencing in the MLL-AF9+ THP-1 cell line resulted in an impairment of their growth and clonogenicity. Taken together, our data highlight ZNF521 role in the control of self-renewal and in the immature compartment of malignant hematopoiesis, which, by altering the gene expression landscape, contributes to the development and/or maintenance of AML acting in concert with the MLL-AF9 fusion oncogene. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=AML" title="AML">AML</a>, <a href="https://publications.waset.org/abstracts/search?q=human%20zinc%20finger%20protein%20521%20%28hZNF521%29" title=" human zinc finger protein 521 (hZNF521)"> human zinc finger protein 521 (hZNF521)</a>, <a href="https://publications.waset.org/abstracts/search?q=mixed%20lineage%20leukemia%20gene%20%28MLL%29%20AF9%20%28MLLT3%20or%20LTG9%29" title=" mixed lineage leukemia gene (MLL) AF9 (MLLT3 or LTG9)"> mixed lineage leukemia gene (MLL) AF9 (MLLT3 or LTG9)</a>, <a href="https://publications.waset.org/abstracts/search?q=cord%20blood-derived%20hematopoietic%20stem%20cells%20%28CB-CD34%2B%29" title=" cord blood-derived hematopoietic stem cells (CB-CD34+)"> cord blood-derived hematopoietic stem cells (CB-CD34+)</a> </p> <a href="https://publications.waset.org/abstracts/157048/the-stem-cell-transcription-co-factor-znf521-sustains-mll-af9-fusion-protein-in-acute-myeloid-leukemias-by-altering-the-gene-expression-landscape" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/157048.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">110</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7638</span> Oxidative and Hormonal Disruptions Underlie Bisphenol A: Induced Testicular Toxicity in Male Rabbits</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kadry%20M.%20Sadek">Kadry M. Sadek</a>, <a href="https://publications.waset.org/abstracts/search?q=Tarek%20K.%20Abouzed"> Tarek K. Abouzed</a>, <a href="https://publications.waset.org/abstracts/search?q=Mousa%20A.%20Ayoub"> Mousa A. Ayoub</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The presence of endocrine-disrupting compounds, such as bisphenol A (BPA), in the environment can cause serious health problems. However, there are controversial opinions. This study investigated the reproductive, metabolic, oxidative and immunologic-disrupting effects of bisphenol A in male rabbits. Rabbits were divided into five groups. The first four rabbit groups were administered oral BPA (1, 10, 50, or 100 mg/kg/day) for ten weeks. The fifth group was administered corn oil as the vehicle. BPA significantly decreased serum testosterone, estradiol and the free androgen index (FAI) and significantly increased sex hormone binding globulin (SHBG) compared with the placebo group. The higher doses of BPA showed a significant decrease in follicular stimulating hormone (FSH) and luteinizing hormone (LH). A significant increase in blood glucose levels was identified in the BPA groups. The non-significant difference in insulin levels is a novel finding. The cumulative testicular toxicity of BPA was clearly demonstrated by the dose-dependent decrease in absolute testes weight, primary measures of semen quality and a significant increase in testicular malonaldehyde (MDA). Moreover, BPA significantly decreased total antioxidant capacity (TAC) and significantly increased immunoglobulin G (IgG) at the highest concentration. Our results suggest that BPA, especially at higher doses, is associated with many adverse effects on metabolism, oxidative stress, immunity, sperm quality and markers of androgenic action. These results may reflect the estrogenic effects of BPA, which we hypothesize could be related, in part, to an inhibitory effect on testicular steroidogenesis. The induction of oxidative stress by BPA may play an additional role in testicular toxicity. These results suggest that BPA poses a threat to endocrine and reproductive functions. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bisphenol%20A" title="bisphenol A">bisphenol A</a>, <a href="https://publications.waset.org/abstracts/search?q=oxidative%20stress" title=" oxidative stress"> oxidative stress</a>, <a href="https://publications.waset.org/abstracts/search?q=rabbits" title=" rabbits"> rabbits</a>, <a href="https://publications.waset.org/abstracts/search?q=semen%20quality" title=" semen quality"> semen quality</a>, <a href="https://publications.waset.org/abstracts/search?q=steroidogenesis" title=" steroidogenesis"> steroidogenesis</a> </p> <a href="https://publications.waset.org/abstracts/14594/oxidative-and-hormonal-disruptions-underlie-bisphenol-a-induced-testicular-toxicity-in-male-rabbits" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/14594.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">294</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7637</span> Applying Cationic Porphyrin Derivative 5, 10-Dihexyl-15, 20bis Porphyrin, as Transfection Reagent for Gene Delivery into Mammalian Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hajar%20Hosseini%20Khorami">Hajar Hosseini Khorami</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Porphyrins are organic, aromatic compounds found in heme, cytochrome, cobalamin, chlorophyll , and many other natural products with essential roles in biological processes that their cationic forms have been used as groups of favorable non-viral vectors recently. Cationic porphyrins are self-chromogenic reagents with a high capacity for modifications, great interaction with DNA and protection of DNA from nuclease during delivery of it into a cell with low toxicity. In order to have high efficient gene transfection into the cell while causing low toxicity, genetically manipulations of the non-viral vector, cationic porphyrin, would be useful. In this study newly modified cationic porphyrin derivative, 5, 10-dihexyl-15, 20bis (N-methyl-4-pyridyl) porphyrin was applied. Cytotoxicity of synthesized cationic porphyrin on Chinese Hamster Ovarian (CHO) cells was evaluated by using MTT assay. This cationic derivative is dose-dependent, with low cytotoxicity at the ranges from 100 μM to 0.01μM. It was uptake by cells at high concentration. Using direct non-viral gene transfection method and different concentration of cationic porphyrin were tested on transfection of CHO cells by applying derived transfection reagent with X-tremeGENE HP DNA as a positive control. However, no transfection observed by porphyrin derivative and the parameters tested except for positive control. Results of this study suggested that applying different protocol, and also trying other concentration of cationic porphyrins and DNA for forming a strong complex would increase the possibility of efficient gene transfection by using cationic porphyrins. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cationic%20porphyrins" title="cationic porphyrins">cationic porphyrins</a>, <a href="https://publications.waset.org/abstracts/search?q=gene%20delivery" title=" gene delivery"> gene delivery</a>, <a href="https://publications.waset.org/abstracts/search?q=non-viral%20vectors" title=" non-viral vectors"> non-viral vectors</a>, <a href="https://publications.waset.org/abstracts/search?q=transfection%20reagents" title=" transfection reagents"> transfection reagents</a> </p> <a href="https://publications.waset.org/abstracts/88175/applying-cationic-porphyrin-derivative-5-10-dihexyl-15-20bis-porphyrin-as-transfection-reagent-for-gene-delivery-into-mammalian-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/88175.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">199</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7636</span> Utilizing the RhlR/RhlI Quorum Sensing System to Express the ß-Galactosidase Reporter Gene by Using the N-Butanoyl Homoserine Lactone and N-Hexanoyl Homoserine Lactone</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ngoc%20Tu%20Truong">Ngoc Tu Truong</a>, <a href="https://publications.waset.org/abstracts/search?q=Nuong%20T.%20Bui"> Nuong T. Bui</a>, <a href="https://publications.waset.org/abstracts/search?q=Ben%20Rao"> Ben Rao</a>, <a href="https://publications.waset.org/abstracts/search?q=Ya%20L.%20Shen"> Ya L. Shen</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Quorum sensing is a phenomenon present in many gram-negative bacteria that allows bacterial communication and controlled expression of a large suite of genes through quorum sensing signals - N-acyl homoserine lactones (AHLs). In order to investigate the ability of the rhlR/rhlI quorum sensing system in Pseudomonas aeruginosa to express the ß-Galactosidase reporter gene, an engineered E. coli strain EpHL02, was genetically engineered. This engineered E. coli strain EpHL02 responded to the presence of the N-butanoyl homoserine lactone and N-hexanoyl homoserine lactone to express the ß-Galactosidase reporter gene at a concentration limit of 5x10⁻⁸ M. This was also found to be comparable to AHLs extraction from Serratia marcescens H31. Moreover, we examined this ability of this engineered E. coli strain for respond of AHLs from extractions of Pseudomonas aeruginosa ATCC9027. The results demonstrated that the rhlR/rhlI quorum sensing system can express the ß-Galactosidase reporter gene by using the N-butanoyl homoserine lactone, N-hexanoyl homoserine lactone and AHLs from extractions of Serratia marcescens H31 and Pseudomonas aeruginosa ATCC9027 in the engineered E. coli strain EpHL02. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=N-butanoyl%20homoserine%20lactone" title="N-butanoyl homoserine lactone">N-butanoyl homoserine lactone</a>, <a href="https://publications.waset.org/abstracts/search?q=C4-HSL" title=" C4-HSL"> C4-HSL</a>, <a href="https://publications.waset.org/abstracts/search?q=N-hexanoyl%20homoserine%20lactone" title=" N-hexanoyl homoserine lactone"> N-hexanoyl homoserine lactone</a>, <a href="https://publications.waset.org/abstracts/search?q=C6-HSL" title=" C6-HSL"> C6-HSL</a>, <a href="https://publications.waset.org/abstracts/search?q=Pseudomonas%20aeruginosa" title=" Pseudomonas aeruginosa"> Pseudomonas aeruginosa</a>, <a href="https://publications.waset.org/abstracts/search?q=quorum%20sensing" title=" quorum sensing"> quorum sensing</a>, <a href="https://publications.waset.org/abstracts/search?q=Serratia%20marcescens" title=" Serratia marcescens"> Serratia marcescens</a>, <a href="https://publications.waset.org/abstracts/search?q=%C3%9F-galactosidase%20reporter%20gene" title=" ß-galactosidase reporter gene"> ß-galactosidase reporter gene</a> </p> <a href="https://publications.waset.org/abstracts/90029/utilizing-the-rhlrrhli-quorum-sensing-system-to-express-the-ss-galactosidase-reporter-gene-by-using-the-n-butanoyl-homoserine-lactone-and-n-hexanoyl-homoserine-lactone" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/90029.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">305</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7635</span> Establishing a Genetic Link between Fat Mass and Obesity Associated and Vitamin D Receptor Gene Polymorphisms and Obesity in the Emirati Population</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Saad%20Mahmud%20Khan">Saad Mahmud Khan</a>, <a href="https://publications.waset.org/abstracts/search?q=Sarah%20El%20Hajj%20Chehadeh"> Sarah El Hajj Chehadeh</a>, <a href="https://publications.waset.org/abstracts/search?q=Mehera%20Abdulrahman"> Mehera Abdulrahman</a>, <a href="https://publications.waset.org/abstracts/search?q=Wael%20Osman"> Wael Osman</a>, <a href="https://publications.waset.org/abstracts/search?q=Habiba%20Al%20Safar"> Habiba Al Safar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Obesity is a non-communicable disease that is widely prevalent with approximately 600 million people classified as obese worldwide. Its etiology is multifactorial and involves a complex interplay between genes and the environment. Over the past few decades, obesity rates among the Emirati population have been increasing. The aim of this study was to investigate the association of candidate gene single nucleotide polymorphisms (SNPs), namely the fat mass and obesity associated (FTO) gene SNP rs9939609 and Vitamin D Receptor (VDR) gene SNP rs1544410, with obesity in the UAE population. Methods: This is a case-control study in which 414 individuals were enrolled during their routine visit to endocrinology clinics in Abu Dhabi, United Arab Emirates between the period of June 2012 and December 2013. Several biochemical tests and clinical assessments along with a lifestyle questionnaire for each participant were completed at the clinic. Genomic DNA was extracted from saliva samples of 201 obese, 114 overweight and 99 normal subjects. Genotyping for the variants was performed using TaqMan assay. Results: The mean Body Mass Index (BMI) ± SD for the obese, overweight, and normal subjects was 35.76 ± 4.54, 27.53 ± 1.45 and 22.69 ± 1.84 kg/m2, respectively. Increasing BMI values were associated with an increase in values for systolic blood pressure, diastolic blood pressure, HbA1c, and triglycerides. The SNP rs9939609 in the FTO gene was found to be significantly associated with the BMI (p=0.028), with the minor allele A having a clear additive effect on BMI values. No significant association was detected between BMI and rs1544410 of the VDR gene. Conclusions: Our study findings indicate that the minor allele A of the rs9939609 has a significant association with increasing BMI values. In addition, our findings support the fact that increasing BMI is associated with increasing risks of other comorbidities such as higher blood pressure, poorer glycemic control and higher triglycerides. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=body%20mass%20index" title="body mass index">body mass index</a>, <a href="https://publications.waset.org/abstracts/search?q=FTO%20gene" title=" FTO gene"> FTO gene</a>, <a href="https://publications.waset.org/abstracts/search?q=obesity" title=" obesity"> obesity</a>, <a href="https://publications.waset.org/abstracts/search?q=rs9939609" title=" rs9939609"> rs9939609</a>, <a href="https://publications.waset.org/abstracts/search?q=United%20Arab%20Emirates" title=" United Arab Emirates"> United Arab Emirates</a> </p> <a href="https://publications.waset.org/abstracts/75440/establishing-a-genetic-link-between-fat-mass-and-obesity-associated-and-vitamin-d-receptor-gene-polymorphisms-and-obesity-in-the-emirati-population" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/75440.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">222</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7634</span> Ordinary Differentiation Equations (ODE) Reconstruction of High-Dimensional Genetic Networks through Game Theory with Application to Dissecting Tree Salt Tolerance</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Libo%20Jiang">Libo Jiang</a>, <a href="https://publications.waset.org/abstracts/search?q=Huan%20Li"> Huan Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Rongling%20Wu"> Rongling Wu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Ordinary differentiation equations (ODE) have proven to be powerful for reconstructing precise and informative gene regulatory networks (GRNs) from dynamic gene expression data. However, joint modeling and analysis of all genes, essential for the systematical characterization of genetic interactions, are challenging due to high dimensionality and a complex pattern of genetic regulation including activation, repression, and antitermination. Here, we address these challenges by unifying variable selection and game theory through ODE. Each gene within a GRN is co-expressed with its partner genes in a way like a game of multiple players, each of which tends to choose an optimal strategy to maximize its “fitness” across the whole network. Based on this unifying theory, we designed and conducted a real experiment to infer salt tolerance-related GRNs for Euphrates poplar, a hero tree that can grow in the saline desert. The pattern and magnitude of interactions between several hub genes within these GRNs were found to determine the capacity of Euphrates poplar to resist to saline stress. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=gene%20regulatory%20network" title="gene regulatory network">gene regulatory network</a>, <a href="https://publications.waset.org/abstracts/search?q=ordinary%20differential%20equation" title=" ordinary differential equation"> ordinary differential equation</a>, <a href="https://publications.waset.org/abstracts/search?q=game%20theory" title=" game theory"> game theory</a>, <a href="https://publications.waset.org/abstracts/search?q=LASSO" title=" LASSO"> LASSO</a>, <a href="https://publications.waset.org/abstracts/search?q=saline%20resistance" title=" saline resistance"> saline resistance</a> </p> <a href="https://publications.waset.org/abstracts/65286/ordinary-differentiation-equations-ode-reconstruction-of-high-dimensional-genetic-networks-through-game-theory-with-application-to-dissecting-tree-salt-tolerance" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/65286.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">639</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7633</span> The Functionality of Ovarian Follicle on Steroid Hormone Secretion under Heat Stress</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Petnamnueng%20Dettipponpong">Petnamnueng Dettipponpong</a>, <a href="https://publications.waset.org/abstracts/search?q=Shuen%20E.%20Chen"> Shuen E. Chen</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Heat stress is known to have negative effects on reproductive functions, such as follicular development and ovulation. This study aimed to investigate the specific effects of heat stress on steroid hormone secretion of ovarian follicle cells, particularly in relation to the expression of Apolipoprotein B (ApoB) and microsomal triglyceride transfer protein (MTP). The aim of the study was to understand the impact of heat stress on steroid hormone secretion in ovarian follicle cells and to explore the role of ApoB and MTP in this process. Primary granulosa and theca cells were collected from follicles and cultured under heat stress conditions (42 °C) for various time periods. Controls were maintained under normal conditions (37.5 °C ). The culture medium was collected at different time points to measure levels of progesterone and estradiol using ELISA kits. ApoB and MTP expression levels were analyzed using homemade antibodies and western blot. Data were assessed by a one-way ANOVA comparison test with Duncan’s new multiple-range test. Results were expressed as mean±S.E. Difference was considered significant at P<0.05. The results showed that heat stress significantly increased progesterone secretion in granulosa cells, with the peak observed after 13 hours of recovery under thermoneutral conditions. Estradiol secretion by theca cells was not affected. Heat stress also had a significant negative effect on granulosa cell viability. Additionally, the expression of ApoB and MTP was found to be differentially regulated by heat stress. ApoB expression in theca cells was transiently promoted, while ApoB expression in granulosa cells was consistently suppressed. MTP expression increased after 5 hours of recovery in both cell types. These findings suggest a mechanism by which chicken follicle cells export cellular lipids as very low-density lipoprotein (VLDL) in response to thermal stress. These contribute to our understanding of the role of ApoB and MTP steroidogenesis and lipid metabolism under heat stress conditions. The study involved the collection of primary granulosa and theca cells, culture under different temperature conditions, and analysis of the culture medium for hormone levels using ELISA kits. ApoB and MTP expression levels were assessed using homemade antibodies and western blot. This study aimed to address the effects of heat stress on steroid hormone secretion in ovarian follicle cells, as well as the role of ApoB and MTP in this process. The study demonstrates that heat stress stimulates steroidogenesis in granulosa cells, affecting progesterone secretion. ApoB and MTP expression were found to be differentially regulated by heat stress, indicating a potential mechanism for the export of cellular lipids in response to thermal stress. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=heat%20stress" title="heat stress">heat stress</a>, <a href="https://publications.waset.org/abstracts/search?q=granulosa%20cells" title=" granulosa cells"> granulosa cells</a>, <a href="https://publications.waset.org/abstracts/search?q=theca%20cells" title=" theca cells"> theca cells</a>, <a href="https://publications.waset.org/abstracts/search?q=steroidogenesis" title=" steroidogenesis"> steroidogenesis</a>, <a href="https://publications.waset.org/abstracts/search?q=chicken" title=" chicken"> chicken</a>, <a href="https://publications.waset.org/abstracts/search?q=apolipoprotein%20B" title=" apolipoprotein B"> apolipoprotein B</a>, <a href="https://publications.waset.org/abstracts/search?q=microsomal%20triglyceride%20transfer%20protein" title=" microsomal triglyceride transfer protein"> microsomal triglyceride transfer protein</a> </p> <a href="https://publications.waset.org/abstracts/181241/the-functionality-of-ovarian-follicle-on-steroid-hormone-secretion-under-heat-stress" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/181241.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">75</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7632</span> Identification and Characterization of Oil-Degrading Bacteria from Crude Oil-Contaminated Desert Soil in Northeastern Jordan</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20Aladwan">Mohammad Aladwan</a>, <a href="https://publications.waset.org/abstracts/search?q=Adelia%20Skripova"> Adelia Skripova</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Bioremediation aspects of crude oil-polluted fields can be achieved by isolation and identification of bacterial species from oil-contaminated soil in order to choose the most active isolates and increase the strength of others. In this study, oil-degrading bacteria were isolated and identified from oil-contaminated soil samples in northeastern Jordan. The bacterial growth count (CFU/g) was between 1.06×10⁵ and 0.75×10⁹. Eighty-two bacterial isolates were characterized by their morphology and biochemical tests. The identified bacterial genera included: Klebsiella, Staphylococcus, Citrobacter, Lactobacillus, Alcaligenes, Pseudomonas, Hafnia, Micrococcus, Rhodococcus, Serratia, Enterobacter, Bacillus, Salmonella, Mycobacterium, Corynebacterium, and Acetobacter. Molecular identification of a universal primer 16S rDNA gene was used to identify four bacterial isolates: Microbacterium esteraromaticum strain L20, Pseudomonas stutzeri strain 13636M, Klebsilla pneumoniae, and uncultured Klebsilla sp., known as new strains. Our results indicate that their specific oil-degrading bacteria isolates might have a high strength of oil degradation from oil-contaminated sites. Staphylococcus intermedius (75%), Corynebacterium xerosis (75%), and Pseudomonas fluorescens (50%) showed a high growth rate on different types of hydrocarbons, such as crude oil, toluene, naphthalene, and hexane. In addition, monooxygenase and catechol 2,3-dioxygenase were detected in 17 bacterial isolates, indicating their superior hydrocarbon degradation potential. Total petroleum hydrocarbons were analyzed using gas chromatography for soil samples. Soil samples M5, M7, and M8 showed the highest levels (43,645, 47,805, and 45,991 ppm, respectively), and M4 had the lowest level (7,514 ppm). All soil samples were analyzed for heavy metal contamination (Cu, Cd, Mn, Zn, and Pb). Site M7 contains the highest levels of Cu, Mn, and Pb, while Site M8 contains the highest levels of Mn and Zn. In the future, these isolates of bacteria can be used for the cleanup of oil-contaminated soil. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bioremediation" title="bioremediation">bioremediation</a>, <a href="https://publications.waset.org/abstracts/search?q=16S%20rDNA%20gene" title=" 16S rDNA gene"> 16S rDNA gene</a>, <a href="https://publications.waset.org/abstracts/search?q=oil-degrading%20bacteria" title=" oil-degrading bacteria"> oil-degrading bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=hydrocarbons" title=" hydrocarbons"> hydrocarbons</a> </p> <a href="https://publications.waset.org/abstracts/155484/identification-and-characterization-of-oil-degrading-bacteria-from-crude-oil-contaminated-desert-soil-in-northeastern-jordan" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/155484.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">126</span> </span> </div> </div> <ul class="pagination"> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=Growth%20Hormone%20Gene&page=7" rel="prev">&lsaquo;</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=Growth%20Hormone%20Gene&page=1">1</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=Growth%20Hormone%20Gene&page=2">2</a></li> <li class="page-item disabled"><span class="page-link">...</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=Growth%20Hormone%20Gene&page=5">5</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=Growth%20Hormone%20Gene&page=6">6</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=Growth%20Hormone%20Gene&page=7">7</a></li> <li class="page-item active"><span class="page-link">8</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=Growth%20Hormone%20Gene&page=9">9</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=Growth%20Hormone%20Gene&page=10">10</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=Growth%20Hormone%20Gene&page=11">11</a></li> <li class="page-item disabled"><span class="page-link">...</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=Growth%20Hormone%20Gene&page=262">262</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=Growth%20Hormone%20Gene&page=263">263</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=Growth%20Hormone%20Gene&page=9" rel="next">&rsaquo;</a></li> </ul> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Explore <li><a href="https://waset.org/disciplines">Disciplines</a></li> <li><a href="https://waset.org/conferences">Conferences</a></li> <li><a href="https://waset.org/conference-programs">Conference Program</a></li> <li><a href="https://waset.org/committees">Committees</a></li> <li><a href="https://publications.waset.org">Publications</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Research <li><a href="https://publications.waset.org/abstracts">Abstracts</a></li> <li><a href="https://publications.waset.org">Periodicals</a></li> <li><a href="https://publications.waset.org/archive">Archive</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Open Science <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Philosophy.pdf">Open Science Philosophy</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Award.pdf">Open Science Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Society-Open-Science-and-Open-Innovation.pdf">Open Innovation</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Postdoctoral-Fellowship-Award.pdf">Postdoctoral Fellowship Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Scholarly-Research-Review.pdf">Scholarly Research Review</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Support <li><a href="https://waset.org/page/support">Support</a></li> <li><a href="https://waset.org/profile/messages/create">Contact Us</a></li> <li><a href="https://waset.org/profile/messages/create">Report Abuse</a></li> </ul> </div> </div> </div> </div> </div> <div class="container text-center"> <hr style="margin-top:0;margin-bottom:.3rem;"> <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" class="text-muted small">Creative Commons Attribution 4.0 International License</a> <div id="copy" class="mt-2">© 2024 World Academy of Science, Engineering and Technology</div> </div> </footer> <a href="javascript:" id="return-to-top"><i class="fas fa-arrow-up"></i></a> <div class="modal" id="modal-template"> <div class="modal-dialog"> <div class="modal-content"> <div class="row m-0 mt-1"> <div class="col-md-12"> <button type="button" class="close" data-dismiss="modal" aria-label="Close"><span aria-hidden="true">×</span></button> </div> </div> <div class="modal-body"></div> </div> </div> </div> <script src="https://cdn.waset.org/static/plugins/jquery-3.3.1.min.js"></script> <script src="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/js/bootstrap.bundle.min.js"></script> <script src="https://cdn.waset.org/static/js/site.js?v=150220211556"></script> <script> jQuery(document).ready(function() { /*jQuery.get("https://publications.waset.org/xhr/user-menu", function (response) { jQuery('#mainNavMenu').append(response); });*/ jQuery.get({ url: "https://publications.waset.org/xhr/user-menu", cache: false }).then(function(response){ jQuery('#mainNavMenu').append(response); }); }); </script> </body> </html>