CreMap™ Epitope Mapping & Discovery Services

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We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects. </p> <b class="Highlights_tit">Highlights</b> <ul class="HighlightsArea"> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Abouts-menu-lights-pic-1.jpg" srcset="static/images/Abouts-menu-lights-pic-1.webp 1x" width="200" height="131" loading="lazy"><a href="aboutus.html" class="bannerbutton"> <i class="fa fa-angle-right"></i>About CBL </a> </li> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Abouts-menu-lights-pic-2.jpg" srcset="static/images/Abouts-menu-lights-pic-2.webp 1x" width="200" height="131" loading="lazy"><a href="contactus.html" class="bannerbutton"> <i class="fa fa-angle-right"></i>Contact Us </a> </li> </ul> </li> </ul> </div> </div> </li> </ul> <script> window.addEventListener('load',function(){ var arr = ['/drug-discovery/therapeutics/','/bioconjugation/','/adc/']; $('.mennav ul li a').each(function(){ var $this = $(this); var href = $this.attr("href").toLocaleLowerCase(); $.each(arr, function(index, value) { if (href && href.startsWith(value)) { $this.attr('target', '_blank'); } }); }); }); </script> </div> <div class="top-search search-group"> <div class="col-md-5 col-sm-6"> <label class="radio-inline"><input type="radio" name="search" class="rdo_productsearch" value="product" data-type="product" placeholder="Please input cat#, product name, abbr, or keywords."> Product</label> <label class="radio-inline"><input type="radio" name="search" class="rdo_servicesearch" value="service" data-type="service" placeholder="Please input your keywords."> Service</label> <label class="radio-inline"><input type="radio" name="search" class="rdo_googlesearch" value="google" data-type="google" placeholder="Please input your keywords." checked="checked"> Keyword</label> </div> <div class="col-md-7 col-sm-6 search-div"> <input id="keys" name="keys" placeholder="Please input your keywords." class="search-input"> <div class="selectItems"></div> <span class="search_btn"><i class="fa fa-search"></i></span> </div> </div> </div> <div class="searchShow"> <div class="searchtopbg"></div> <span class="searchclosed"><i class="fa fa-times"></i>Close</span> <div class="addsearch search-group"></div> </div> <div class="banner_inpage services_bg_2"> <div class="auto-container small_content-width about_container"> <ul class="bread-crumb clearfix"> <li><a href="/">Home</a></li> <li><a href="javascrpt:void(0)">Characterization</a></li><li><a href="javascrpt:void(0)">Binding Analysis</a></li> <li>Epitope Mapping</li> </ul> <p>"Creative Biolabs is committed to providing highly customized comprehensive solutions with the best quality to advance our global clients’ projects."</p> </div> </div> <script> window.addEventListener('load', function () { if (($('.bread-crumb li:last-child').text()) == "Peer-Reviewed Publications") { $(".banner_inpage").addClass("peer-reviewed-publications_bg").removeClass("services_bg_2"); } if ($(window).hasClass('bread-crumb')) { if ($(".bread-crumb").html().indexOf("RESOURCE") > -1) { $(".pronote").hide(); } if ($(".bread-crumb").html().indexOf("Careers") > -1) { $(".pronote").hide(); } } }); </script> <div class="small_content-width services-container"> <div class="col-md-9"> <div class="inpage_section_title"> <h1>CreMap™ Epitope Mapping & Discovery Services</h1> </div> <div class="page-tab"> <span onclick="window.location.href=window.location.pathname+'#tab1'">Background</span> <span onclick="window.location.href=window.location.pathname+'#tab2'">B Cell Epitope Mapping</span> <span onclick="window.location.href=window.location.pathname+'#tab3'">T Cell Epitope Discovery</span> <span onclick="window.location.href=window.location.pathname+'#tab4'">Published Data</span> <span onclick="window.location.href=window.location.pathname+'#tab5'">FAQ</span> <span onclick="window.location.href=window.location.pathname+'#tab6'">Resources</span> </div> <script>window.addEventListener('load',function(){page_tab_margin();page_tab_css();})</script> <h2 class="h2_tit page_tab_margin" id="tab1"> Background </h2> <p> With state-of-the-art facilities and enriched experience in antibody engineering, <strong>Creative Biolabs</strong> has developed excellent CreMap™ epitope mapping & discovery platform to provide customers professional technical services in a time- and cost-efficient manner. </p> <p> Epitope, or antigenic determinant, is typically a segment on an antigen that can be recognized by antibodies or T cells for triggering the specific immune responses. Epitope mapping is the process of identifying the binding sites of antibodies (or TCRs) on target antigens, which helps discover the new therapeutics, vaccines, and diagnostics. Identifying epitopes also gives a clue about the mechanism of binding to antibodies or T cell receptors. In most cases, epitope mapping is performed with <a href="epitope-mapping-of-monoclonal-antibodies.html">monoclonal antibodies (mAbs)</a>. </p> <p class="ServiceShowPic"> <img src="static/img/CreMap-Epitope-Mapping-Discovery-Services-1.png" width="400" height="244" loading="lazy" alt="Predicted B cell epitopes of Human papillomavirus type 16 major late capsid protein L1." />Fig.1 Predicted B cell epitopes of Human papillomavirus type 16 major late capsid protein L1. </p> <p> The techniques determining the potent epitopes (both T cell epitopes and B cell epitopes) have been greatly improved by the application and development in immunology for the past years. And these robust techniques could help you to: </p> <ul class="ullist"> <li> Identify high antigenicity epitopes which have the potential to evoke immune responses, in vaccine development for instance. </li> <li> Identify peptide epitope sequences that may lead to the anti-drug antibody (ADA). </li> <li> Identify to binding sites between antigen and antibody in antibody functional studies. </li> <li> Identify the biomarkers or discover key epitopes in the research of cancer, infectious diseases as well as autoimmune diseases, and develop therapeutic antibodies or protein drugs. </li> </ul> <br /> <p> Creative Biolabs' outstanding research group has developed the CreMap™ B Cell Epitope Mapping Platform and CreMap™ T cell Epitope Mapping Platform based on the professional knowledge and enriched experiences. We provide excellent epitope mapping services for our clients based on our advanced platforms. </p> <h2 class="h2_tit page_tab_margin" id="tab2"> <a href="/CreMap-B-Cell-Epitope-Mapping-Service.html">CreMap™ B Cell Epitope Mapping Platform</a> </h2> <p> The B cell epitopes are the binding sites of an antigen recognized by the corresponding antibodies. Based on the different binding characteristics, B cell epitopes contain two categories: linear epitopes and conformation epitopes. The linear epitopes are continuous sequences of amino acid on peptide chains, whereas the conformation epitopes act as sequence discontinuous amino acid chains but spatially adjacent. </p> <p> Our expert group in Creative Biolabs has developed an advanced B cell epitope mapping platform, which contains several advanced technologies for mapping both conformational and linear epitopes in a high-throughput and efficient way. <a href="/CreMap-B-Cell-Epitope-Mapping-Service.html" rel="nofollow">Read more…</a> </p> <h2 class="h2_tit page_tab_margin" id="tab3"> <a href="/CreMap-T-Cell-Epitope-Discovery-Service.html">CreMap™ T Cell Epitope Discovery Platform</a> </h2> <p> In the CreMap™ T cell epitope discovery platform, Creative Biolabs has developed an exceptional MHC-peptide binding assay for determining the potent binding sites of the T cell with effective approaches. Compared with other ineffective and time-consume strategies, this platform provides a cost-effective solution for your epitope detection when you are attempting to design a peptide vaccine or develop a protein drug to certain diseases. <a href="/CreMap-T-Cell-Epitope-Discovery-Service.html " rel="nofollow">Read more…</a> </p> <p> Over the past few years,<strong> Creative Biolabs</strong> has been providing best-in-class epitope mapping service by our excellent CreMap™ platform, aiming to guide your epitope mapping and discovery research. If you are interested in our service, please <a class="inquiry-button">contact us</a> for more detail information. </p> <p class="ServiceShowPic"> <img src="static/img/CreMap-Epitope-Mapping-Discovery-Services-new.png" width="400" height="212" loading="lazy" alt="Predicted B cell epitopes of Human papillomavirus type 16 major late capsid protein L1." /> </p> <h2 class="h2_tit page_tab_margin" id="tab4"> Published Data </h2> <ul class="ullist"> <li> <strong>Epitope Mapping Techniques for Viral Antigen Discovery</strong> </li> </ul> <p class="show-center"> <img loading="lazy" src="static/img/1-2-8-3-cremap-epitope-mapping-discovery-services-2.jpg" width="450" height="298" alt="" />Fig. 2 Multiplexed serology for viral epitope mapping. (Diya Hu, 2023) </p> <p> The article explores the research significance of massively multiplexed epitope mapping techniques for viral antigen discovery, emphasizing their application in immunotherapy and vaccine development. Epitope mapping's main goal is to identify the minimal regions on antigens that immune cells recognize, which is crucial for developing effective vaccines and therapies against viruses. The study demonstrates the use of high-throughput technologies, such as bacteriophage-display libraries and deep mutational scanning, to identify and characterize viral epitopes comprehensively. These methods allow for the rapid screening of a vast number of epitopes, aiding in the quick response to viral mutations and the design of targeted immunotherapies. </p> <div class="reference"> <strong>Reference</strong> <ol class="ollist decimal"> <li> Hu, Diya, and Aaron T. Irving. "Massively-multiplexed epitope mapping techniques for viral antigen discovery." <em>Frontiers in Immunology</em> 14 (2023): 1192385. </li> </ol> </div> <script type="text/javascript"> window.addEventListener('load',function(){ faqlist(4); }); </script> <h2 class="h2_tit page_tab_margin" id="tab5"> FAQ </h2> <ol class="ollist faqlist"> <li> <span class="sFAQTit">What is epitope mapping?</span> <p> Epitope mapping is the process of identifying the specific parts, or epitopes, of antigens that are recognized by immune cells, such as antibodies or T-cell receptors. This technique is crucial for understanding how pathogens interact with the immune system, designing vaccines, and developing diagnostic tests. </p> </li> <li> <span class="sFAQTit">What techniques are used for epitope mapping?</span> <p> Several techniques are used for epitope mapping, including X-ray crystallography, nuclear magnetic resonance (NMR), mass spectrometry, phage display, yeast display, and computational prediction models. These methods help reveal the structural and functional characteristics of epitopes. </p> </li> <li> <span class="sFAQTit">How does phage display aid in epitope discovery?</span> <p> Phage display involves expressing peptide libraries on the surface of bacteriophages, which are then screened against antibodies or T-cell receptors to identify binding interactions. This technique is highly versatile and allows for the screening of millions of peptides, making it a powerful tool for epitope discovery. </p> </li> <li> <span class="sFAQTit">Can epitope mapping be used to improve existing vaccines?</span> <p> Epitope mapping can be used to enhance the effectiveness of existing vaccines by identifying new, more potent or broadly reactive epitopes. This information can be used to update vaccine formulations to cover emerging pathogen variants or to improve immune responses in different populations. </p> </li> <li> <span class="sFAQTit">What is the role of computational biology in epitope mapping?</span> <p> Computational biology plays a crucial role in epitope mapping by using algorithms and models to predict epitope structures and their interactions with antibodies or T-cell receptors. These tools can significantly speed up the discovery process and reduce the need for extensive laboratory testing. </p> </li> <li> <span class="sFAQTit">How do epitope mapping techniques differ for B-cell and T-cell epitopes?</span> <p> B-cell epitopes are often mapped using methods that identify continuous or conformational epitopes on the surface of proteins, such as phage display or mass spectrometry. T-cell epitopes, which must be presented by MHC molecules, are often mapped using peptide-MHC multimer technologies or cellular assays that measure T-cell responses. </p> </li> <li> <span class="sFAQTit">What challenges are associated with epitope mapping?</span> <p> Epitope mapping faces several challenges, including the complexity of protein structures, the vast diversity of epitopes, and the difficulty of predicting which epitopes will elicit a protective immune response. Additionally, technical limitations in accurately mimicking the natural processing and presentation of epitopes can affect the accuracy of mapping. </p> </li> <li> <span class="sFAQTit">How is epitope mapping used in personalized medicine?</span> <p> In personalized medicine, epitope mapping can be used to tailor immunotherapies to individual patients, especially in cancer treatment. By identifying tumor-specific epitopes, therapies can be designed to target only cancer cells, minimizing damage to normal tissues and improving treatment outcomes. </p> </li> <li> <span class="sFAQTit">What advancements are expected in the field of epitope mapping?</span> <p> Future advancements in epitope mapping are likely to come from improvements in high-throughput screening technologies, more accurate computational models, and better understanding of the immune system's interaction with different epitopes. These developments will enhance the speed and precision of vaccine design and immunotherapy development. </p> </li> </ol> <h2 class="h2_tit page_tab_margin" id="tab6"> Resources </h2> <p> Use the resources in our library to help you understand your options and make critical decisions for your study. </p> <div class="tab_nav"> <span class="tab_choosebg">Videos</span><span>Articles</span> </div>  <div class="tab_cont" style="display:block;"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/immunogenicity-overview-of-therapeutic-biologics.jpg" alt="" /> </div> <a href="https://youtu.be/J430752_dTo" target="_blank">Immunogenicity overview of therapeutic biologics</a> </div> </div> </div>  <div class="tab_cont"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="static/img/4-2-14-immune-monitoring-for-immunogenicity-assessment-1.jpg" alt="" /> </div> <a href="/immune-monitoring-for-immunogenicity-assessment.html">Immune Monitoring for Immunogenicity Assessment</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="static/img/4-2-14-current-immunogenicity-evaluation-systems-a-deep-dive-by-creative-biolabs-1.jpg" alt="" /> </div> <a href="/current-immunogenicity-evaluation-systems.html">Current Immunogenicity Evaluation Systems: A Deep Dive by Creative Biolabs</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="static/img/4-2-14-1-in-silico-immunogenicity-assessment-the-leading-edge-approach-to-drug-development-1.jpg" alt="" /> </div> <a href="/in-silico-immunogenicity-assessment-the-leading-edge-approach-to-drug-development.html"><i>In Silico</i> Immunogenicity Assessment: The Leading-edge Approach to Drug Development</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="static/img/4-2-14-4-how-to-evaluate-the-immunogenicity-of-therapeutic-protein-drugs-in-vivo-1.jpg" alt="" /> </div> <a href="/how-to-evaluate-the-immunogenicity-of-therapeutic-protein-drugs-in-vivo.html">How to Evaluate the Immunogenicity of Therapeutic Protein Drugs <i>In Vivo</i></a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="static/img/4-2-14-5-the-origin-and-detection-methods-of-anti-drug-antibodies-1.jpg" alt="" /> </div> <a href="/the-origin-and-detection-methods-of-anti-drug-antibodies.html">The Origin and Detection Methods of Anti-Drug Antibodies</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="static/img/4-2-14-1-8-immunogenic-risk-assessment-of-therapeutic-proteins-1.jpg" alt="" /> </div> <a href="/immunogenic-risk-assessment-of-therapeutic-proteins.html">Immunogenic Risk Assessment of Therapeutic Proteins</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="static/img/anti-drug-antibody-introduction-1.jpg" alt="" /> </div> <a href="/anti-drug-antibody-introduction.html">Anti-Drug Antibody Introduction</a> </div> </div> </div> <script> window.addEventListener('load',function(){ // $(function () { $(".tab_nav span").click(function(){ var eqindex=$(this).index(); $(this).addClass("tab_choosebg"); $(this).siblings("span").removeClass("tab_choosebg"); $(".tab_cont").hide().eq(eqindex).show(); }) // }) }); </script><br> <p class="pronote"><i class="fa fa-exclamation-triangle"></i>All listed services and products are For Research Use Only. 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