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不可消化的水苏糖结合小肠上皮膜上HSP90β调节外泌体miRNA—小柯机器人—科学网
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However, our research has reframed this understanding by uncovering a new function of oligosaccharide stachyose, which binds hydrophobic residues of membranous HSP90β on small intestinal epithelial cells, thus reprograming the exosomal miRNA profile. CRISPR-Cas9-mediated HSP90β knockout abolished the accumulation of stachyose on cell membrane and its regulatory effects on these miRNAs. Notably, stachyose’s regulation on these miRNAs is independent of its prebiotic role, as evidenced by the observation of stachyose-altered fecal miRNAs in pseudo-germ-free mice. These stachyose-altered miRNAs further shaped colonic microbiome, especially harboring Lactobacillus in mice. Thereinto, miR-30a-5p that was downregulated (Log2FC < 2) in both mice and human feces following stachyose treatment could specifically suppress the growth of Lactobacillus reuteri. These findings build a new regulatory axis of stachyose-intestinal miRNAs-gut microbiota and unveil a previously unknown mechanism underlying the direct “talk” of oligosaccharides to intestine epithelium via membranous HSP90β.</p> <p> DOI: 10.1016/j.cmet.2024.10.012</p> <p> Source: <a href="https://www.cell.com/cell-metabolism/abstract/S1550-4131(24)00408-X">https://www.cell.com/cell-metabolism/abstract/S1550-4131(24)00408-X</a></p> </p> <div class="remarks">期刊信息</div> <div class="dd"><p> <span style="font-weight: bold;">Cell Metabolism：</span>《细胞—代谢》，创刊于2005年。隶属于细胞出版社，最新IF：31.373<br /> <span style="font-weight: bold;">官方网址：</span><a href="https://www.cell.com/cell-metabolism/home" target="_blank">https://www.cell.com/cell-metabolism/home</a><br /> <span style="font-weight: bold;">投稿链接：</span><a href="https://www.editorialmanager.com/cell-metabolism/default.aspx" target="_blank">https://www.editorialmanager.com/cell-metabolism/default.aspx</a></p> </div> </div> </div> <style> #footer{ background:#890f0e; text-align:center; padding:10px; color:#FFF; font-size:12px;} #footer a{color:#FFF; font-size:12px;} </style> <div id="footer"><a href="http://www.sciencenet.cn/aboutus/" target="_blank">关于我们</a> | <a href="http://www.sciencenet.cn/shengming.aspx" target="_blank">网站声明</a> | <a href="http://www.sciencenet.cn/tiaokuang.aspx" target="_blank">服务条款</a> | <a href="http://www.sciencenet.cn/contact.aspx" target="_blank">联系方式</a> | <a href="http://www.sciencenet.cn/RSS.aspx">RSS</a> | <a href="mailto:jubao@stimes.cn">举报</a> | <a href="https://stimes.sciencenet.cn/">中国科学报社</a> <br> <a href="https://beian.miit.gov.cn" target="_blank" > 京ICP备07017567号-12 </a> 互联网新闻信息服务许可证10120230008 京公网安备 11010802032783<br>Copyright @ 2007-<script type="text/javascript">var Date22 = new Date();var year22 = Date22.getFullYear();document.write(year22);</script> 中国科学报社 All Rights Reserved<br> 地址：北京市海淀区中关村南一条乙三号 邮箱：blog@stimes.cn <br> <span style="display:none"> <script type="text/javascript"> var _bdhmProtocol = (("https:" == document.location.protocol) ? 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