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b</title> <meta name="description" content="Search results for: cucurbitacin b"> <meta name="keywords" content="cucurbitacin b"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="cucurbitacin b"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 6</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: cucurbitacin b</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> Comparison of Overall Sensitivity of Meloidogyne incognita to Pure Cucurbitacins and Cucurbitacin-Containing Crude Extracts</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zakheleni%20P.%20Dube">Zakheleni P. Dube</a>, <a href="https://publications.waset.org/abstracts/search?q=Phatu%20W.%20Mashela"> Phatu W. Mashela </a> </p> <p class="card-text"><strong>Abstract:</strong></p> The Curve-fitting Allelochemical Response Data (CARD) model had been adopted as a valuable tool in enhancing the understanding of the efficacy of cucurbitacin-containing phytonematicides on the suppression of nematodes. In most cases, for registration purposes, the active ingredients should be in purified form. Evidence in other phytonematicides suggested that purified active ingredients were less effective in suppression of nematodes. The objective of this study was to use CARD model to compare the overall sensitivities of Meloidogyne incognita J2 hatch, mobility and mortality to Nemarioc-AL phytonematicides, cucurbitacin A, Nemafric-BL phytonematicide and cucurbitacin B. Meloidogyne incognita eggs and J2 were exposed to 0.00, 0.50, 1.00, 1.50, 2.00, 2.50, 3.00, 3.50, 4.00, 4.50 and 5.00% of each phytonematicide, whereas in purified form the concentrations were 0.00, 0.25, 0.50, 0.75, 1.00, 1.25, 1.50, 1.75, 2.00, 2.25 and 2.50 μg.mL⁻¹. The exposure period to each concentration was 24-, 48- and 72-h. The overall sensitivities of J2 hatch to Nemarioc-AL phytonematicide, cucurbitacin A, Nemafric-BL phytonematicide and cucurbitacin B were 1, 30, 5 and 2 units, respectively, whereas J2 mobiltity were 3, 17, 3 and 6 units, respectively. For J2 mortality overall sensitivities to Nemarioc-AL phytonematicide, cucurbitacin A, Nemafric-BL phytonematicide and cucurbitacin B were 2, 4, 1 and 4 units, respectively. In conclusion, the two crude extracts, Nemarioc-AL and Nemafric-BL phytonematicides were generally more potent to M. incognita compared to their pure active ingredients. The crude plant extract preparation is easy, and they could be an ideal tactic for the management of nematodes in resource poor farming communities. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Botanicals" title="Botanicals">Botanicals</a>, <a href="https://publications.waset.org/abstracts/search?q=cucumin" title=" cucumin"> cucumin</a>, <a href="https://publications.waset.org/abstracts/search?q=leptodermin" title=" leptodermin"> leptodermin</a>, <a href="https://publications.waset.org/abstracts/search?q=plant%20extracts" title=" plant extracts"> plant extracts</a>, <a href="https://publications.waset.org/abstracts/search?q=triterpenoids" title=" triterpenoids"> triterpenoids</a> </p> <a href="https://publications.waset.org/abstracts/72585/comparison-of-overall-sensitivity-of-meloidogyne-incognita-to-pure-cucurbitacins-and-cucurbitacin-containing-crude-extracts" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/72585.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">209</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> Influence of Cucurbitacin-Containing Phytonematicides on Nematode Biocontrol Agent: Trichoderma harzianum</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jacqueline%20T.%20Madaure">Jacqueline T. Madaure</a>, <a href="https://publications.waset.org/abstracts/search?q=Phatu%20W.%20Mashela"> Phatu W. Mashela</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cucurbitacin-containing phytonematicides consistently suppress root-knot (Meloidogyne species) nematode population densities. However, the impact of these products on nematode biocontrol agents is not documented. The objective of this study was to determine the influence of Nemarioc-AL and Nemafric-BL phytonematicides on growth of Trichoderma harzianum under in vitro conditions. The two phytonematicides were separately prepared to concentrations of 3% and used in poison plate assays. After exposure at different times from 0 to 72 h, there was 100% mycelial growth of T. harzianum. In conclusion, at the recommended concentrations of phytonematicides used in managing nematode population densities, there was no evidence of suppressive effects on growth of T. harzianum by the two phytonematicides. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=botanicals" title="botanicals">botanicals</a>, <a href="https://publications.waset.org/abstracts/search?q=crude%20extracts" title=" crude extracts"> crude extracts</a>, <a href="https://publications.waset.org/abstracts/search?q=cucumis%20africanus" title=" cucumis africanus"> cucumis africanus</a>, <a href="https://publications.waset.org/abstracts/search?q=cucumis%20myriocarpus" title=" cucumis myriocarpus"> cucumis myriocarpus</a>, <a href="https://publications.waset.org/abstracts/search?q=cucurbitacin%20a" title=" cucurbitacin a"> cucurbitacin a</a>, <a href="https://publications.waset.org/abstracts/search?q=cucurbitacin%20b" title=" cucurbitacin b"> cucurbitacin b</a>, <a href="https://publications.waset.org/abstracts/search?q=ethnomedicinal%20plants" title=" ethnomedicinal plants"> ethnomedicinal plants</a> </p> <a href="https://publications.waset.org/abstracts/72590/influence-of-cucurbitacin-containing-phytonematicides-on-nematode-biocontrol-agent-trichoderma-harzianum" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/72590.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">219</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Developing Cucurbitacin a Minimum Inhibition Concentration of Meloidogyne Incognita Using a Computer-Based Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zakheleni%20P.%20Dube">Zakheleni P. Dube</a>, <a href="https://publications.waset.org/abstracts/search?q=Phatu%20W.%20Mashela"> Phatu W. Mashela</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Minimum inhibition concentration (MIC) is the lowest concentration of a chemical that brings about significant inhibition of target organism. The conventional method for establishing the MIC for phytonematicides is tedious. The objective of this study was to use the Curve-fitting Allelochemical Response Data (CARD) to determine the MIC for pure cucurbitacin A on Meloidogyne incognita second-stage juveniles (J2) hatch, immobility and mortality. Meloidogyne incognita eggs and freshly hatched J2 were separately exposed to a series of pure cucurbitacin A concentrations of 0.00, 0.25, 0.50, 0.75, 1.00, 1.25, 1.50, 1.75, 2.00, 2.25 and 2.50 μg.mL⁻¹for 12, 24, 48 and 72 h in an incubator set at 25 ± 2°C. Meloidogyne incognita J2 hatch, immobility and mortality counts were determined using a stereomicroscope and the significant means were subjected to the CARD model. The model exhibited density-dependent growth (DDG) patterns of J2 hatch, immobility and mortality to increasing concentrations of cucurbitacin A. The average MIC for cucurbitacin A on M. incognita J2 hatch, immobility and mortality were 2.2, 0.58 and 0.63 µg.mL⁻¹, respectively. Meloidogyne incognita J2 hatch had the highest average MIC value followed by mortality and immobility had the least. In conclusion, the CARD model was able to generate MIC for cucurbitacin A, hence it could serve as a valuable tool in the chemical-nematode bioassay studies. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=inhibition%20concentration" title="inhibition concentration">inhibition concentration</a>, <a href="https://publications.waset.org/abstracts/search?q=phytonematicide" title=" phytonematicide"> phytonematicide</a>, <a href="https://publications.waset.org/abstracts/search?q=sensitivity%20index" title=" sensitivity index"> sensitivity index</a>, <a href="https://publications.waset.org/abstracts/search?q=threshold%20stimulation" title=" threshold stimulation"> threshold stimulation</a>, <a href="https://publications.waset.org/abstracts/search?q=triterpenoids." title=" triterpenoids."> triterpenoids.</a> </p> <a href="https://publications.waset.org/abstracts/72586/developing-cucurbitacin-a-minimum-inhibition-concentration-of-meloidogyne-incognita-using-a-computer-based-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/72586.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">190</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Interaction of Cucurbitacin-Containing Phytonematicides and Biocontrol Agents on Cultivated Tomato Plants and Nematode Numbers</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jacqueline%20T.%20Madaure">Jacqueline T. Madaure</a>, <a href="https://publications.waset.org/abstracts/search?q=Phatu%20W.%20Mashela"> Phatu W. Mashela</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Interactive effects of cucurbitacin-containing phytonematicides and biocontrol agents on growth and nematode suppression on tomato (Solanum lycopersicum) had not been documented. The objective of this study was to determine the interactive effects of Nemafric-BL phytonematicide, Trichoderma harzianum and Steinernema feltiae on growth of tomato plants and suppression of root-knot (Meloidogyne species) nematodes. A 2x2x2 trial was conducted using tomato cv. ‘HTX’ on a field infested with Meloidogyne species. The treatments were applied at commercial rates. At 56 days after treatments, interactions were significant (P ≤ 0.05) for selected plant variables, without significant interactions on nematode variables. In conclusion, results of the current study did not support the combination of the test products for nematode suppression, except that some combinations improved plant growth. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cucumis%20africanus" title="cucumis africanus">cucumis africanus</a>, <a href="https://publications.waset.org/abstracts/search?q=cucurbitacin%20b" title=" cucurbitacin b"> cucurbitacin b</a>, <a href="https://publications.waset.org/abstracts/search?q=ethnobotanicals" title=" ethnobotanicals"> ethnobotanicals</a>, <a href="https://publications.waset.org/abstracts/search?q=entomopathogenic%20nematodes" title=" entomopathogenic nematodes"> entomopathogenic nematodes</a>, <a href="https://publications.waset.org/abstracts/search?q=natural%20enemies" title=" natural enemies"> natural enemies</a>, <a href="https://publications.waset.org/abstracts/search?q=plant%20extracts" title=" plant extracts"> plant extracts</a> </p> <a href="https://publications.waset.org/abstracts/72591/interaction-of-cucurbitacin-containing-phytonematicides-and-biocontrol-agents-on-cultivated-tomato-plants-and-nematode-numbers" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/72591.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">195</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Efficacy of Nemafric-BL Phytonematicide on Suppression of Root-Knot Nematodes and Growth of Tomato Plants</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Pontsho%20E.%20Tseke">Pontsho E. Tseke</a>, <a href="https://publications.waset.org/abstracts/search?q=Phatu%20W.%20Mashela"> Phatu W. Mashela</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cucurbitacin-containing phytonematicides had been consistent in suppressing root-knot (Meloidogyne species) when used in dried crude form, with limited evidence whether the efficacy could be affected when fresh fruits were used during fermentation. The objective of this study was to determine the influence of Nemafric-BL phytonematicide prepared using fermented crude extracts of fresh fruit from wild watermelon (Cucumis africanus) on the growth of tomato (Solanum lycopersicum) plants and suppression of Meloidogyne species. Seedlings of tomato cultivar ‘Floradade’ were inoculated with 3 000 eggs and second-stage juveniles (J2) of M. incognita race 2 in pot trials, with treatments comprising 0, 2, 4, 8, 16, 32 and 64 % Nemafric-BL phytonematicide. At 56 days after inoculation, the phytonematicide reduced eggs and J2 in roots by 84-97%, J2 in soil by 49-96% and total nematodes by 70-97%. Plant variables and concentrations of Nemafric-BL phytonematicide exhibited positive quadratic relations, with 74-98% associations. In conclusion, fresh fruit of C. africanus could be used for the preparation of Nemafric-BL phytonematicide, particularly in cases where the dry infrastructure is not available. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Cucurbitacin%20B" title="Cucurbitacin B">Cucurbitacin B</a>, <a href="https://publications.waset.org/abstracts/search?q=density-dependent%20growth" title=" density-dependent growth"> density-dependent growth</a>, <a href="https://publications.waset.org/abstracts/search?q=effective%20microorganisms" title=" effective microorganisms"> effective microorganisms</a>, <a href="https://publications.waset.org/abstracts/search?q=quadratic%20relations" title=" quadratic relations"> quadratic relations</a> </p> <a href="https://publications.waset.org/abstracts/72583/efficacy-of-nemafric-bl-phytonematicide-on-suppression-of-root-knot-nematodes-and-growth-of-tomato-plants" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/72583.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">184</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Influence of Cucurbitacin-Containing Phytonematicides on Growth of Rough Lemon (Citrus jambhiri)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Raisibe%20V.%20Mathabatha">Raisibe V. Mathabatha</a>, <a href="https://publications.waset.org/abstracts/search?q=Phatu%20W.%20Mashela"> Phatu W. Mashela</a>, <a href="https://publications.waset.org/abstracts/search?q=Nehemiah%20M.%20Mokgalong"> Nehemiah M. Mokgalong</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Occasional incidence of phytotoxicity in Nemarioc-BL and Nemafric-AL phytonematicides to crops raises credibility challenges that could negate their registration as commercial products. Responses of plants to phytonematicides are characterized by the existence of stimulation, neutral and inhibition phases, with the mid-point of the former being referred to as the Mean Concentration Stimulation Point (MSCP = Dm + Rh/2). The objective of this study was to determine the MCSP and the overall sensitivity (∑k) of Nemarioc-AL and Nemafric-BL phytonematicides to rough lemon seedling rootstocks using the Curve-fitting Allelochemical Response Dosage (CARD) computer-based model. Two parallel greenhouse experiments were initiated, with seven dilutions of each phytonematicide arranged in a randomised complete block design, replicated nine times. Six-month-old rough lemon seedlings were transplanted into 20-cm-diameter plastic pots, filled with steam-pasteurised river sand (300°C for 3 h) and Hygromix-T growing mixture. Treatments at 0, 2, 4, 8, 16, 32 and 164% dilutions were applied weekly at 300 ml/plant. At 84 days after the treatments, analysis of variance-significant plant variables was subjected to the CARD model to generate appropriate biological indices. Computed MCSP values for Nemarioc-AL and Nemafric-BL phytonematicides on rough lemon were 29 and 38%, respectively, whereas ∑k values were 1 and 0, respectively. At the applied concentrations, rough lemon seedlings were highly sensitive to Nemarioc-AL and Nemafric-BL phytonematicides. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=crude%20extracts" title="crude extracts">crude extracts</a>, <a href="https://publications.waset.org/abstracts/search?q=cucurbitacins" title=" cucurbitacins"> cucurbitacins</a>, <a href="https://publications.waset.org/abstracts/search?q=effective%20microbes" title=" effective microbes"> effective microbes</a>, <a href="https://publications.waset.org/abstracts/search?q=fruit%20extracts" title=" fruit extracts"> fruit extracts</a> </p> <a href="https://publications.waset.org/abstracts/72537/influence-of-cucurbitacin-containing-phytonematicides-on-growth-of-rough-lemon-citrus-jambhiri" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/72537.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">146</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Explore <li><a href="https://waset.org/disciplines">Disciplines</a></li> <li><a href="https://waset.org/conferences">Conferences</a></li> <li><a href="https://waset.org/conference-programs">Conference Program</a></li> <li><a href="https://waset.org/committees">Committees</a></li> <li><a href="https://publications.waset.org">Publications</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Research <li><a href="https://publications.waset.org/abstracts">Abstracts</a></li> <li><a href="https://publications.waset.org">Periodicals</a></li> <li><a href="https://publications.waset.org/archive">Archive</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Open Science <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Philosophy.pdf">Open Science Philosophy</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Award.pdf">Open Science Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Society-Open-Science-and-Open-Innovation.pdf">Open Innovation</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Postdoctoral-Fellowship-Award.pdf">Postdoctoral Fellowship Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Scholarly-Research-Review.pdf">Scholarly Research Review</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Support <li><a href="https://waset.org/page/support">Support</a></li> <li><a href="https://waset.org/profile/messages/create">Contact Us</a></li> <li><a href="https://waset.org/profile/messages/create">Report Abuse</a></li> </ul> </div> </div> </div> </div> </div> <div class="container text-center"> <hr style="margin-top:0;margin-bottom:.3rem;"> <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" class="text-muted small">Creative Commons Attribution 4.0 International License</a> <div id="copy" class="mt-2">© 2024 World Academy of Science, Engineering and Technology</div> </div> </footer> <a href="javascript:" id="return-to-top"><i class="fas fa-arrow-up"></i></a> <div class="modal" id="modal-template"> <div class="modal-dialog"> <div class="modal-content"> <div class="row m-0 mt-1"> <div class="col-md-12"> <button type="button" class="close" data-dismiss="modal" aria-label="Close"><span aria-hidden="true">×</span></button> </div> </div> <div class="modal-body"></div> </div> </div> </div> <script src="https://cdn.waset.org/static/plugins/jquery-3.3.1.min.js"></script> <script src="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/js/bootstrap.bundle.min.js"></script> <script src="https://cdn.waset.org/static/js/site.js?v=150220211556"></script> <script> jQuery(document).ready(function() { /*jQuery.get("https://publications.waset.org/xhr/user-menu", function (response) { jQuery('#mainNavMenu').append(response); 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