Janelia Fluor® 525, NHS ester (6296) by Tocris, Part of Bio-Techne

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class="top_region"><div class="mb-2 o___product-summary"><div id="pp-page-title-brand-wrapper"> <div id="header-subheader" data-section="section-base--header-subheader"> <div class="row" id="pp-page-title-brand-wrapper"> <div class="col-lg-9 col-md-12"> <h1 class="font-weight-bold"> Janelia Fluor<sup>®</sup> 525, NHS ester </h1> <h2 class="font-weight-normal h4"> Tocris Bioscience, part of Bio-Techne <span id="product-code"> | Catalog # <span class="font-weight-bold">6296</span> </span> </h2> <div id="pp-reviews-citations-row"> <div id="header_left" class="row"> <div class="col-auto d-flex align-self-center"> <div> <a href="#product-documents" class="datasheet_link product-documents" rel="nofollow">COA / SDS</a> </div> </div> </div> </div> <div id="new_version_available"> </div> <div class="row mb-2" id="extended-description"> <div class="col-md-10 extended-description-wrapper" id="extended-description-wrapper"> Yellow dye (Reactive group: NHS ester). Application: confocal microscopy, super resolution microscopy (SRM) including dSTORM. Suitable for live cell imaging </div> </div> </div> <div class="col-lg-3 col-md-12"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-tocris.svg" alt="Tocris Bioscience, part of Bio-Techne" class="d-flex h-100 w-100"> </div> </div> </div> </div> <div id="product_alerts_wrapper"><article class="product-data-wrapper"> </article> </div> </div> <div class="row border-top mb-2" id="images_atc_orderinfo_wrapper"><div class="col-xl-9 mt-2" id="image_atc_keydetails_wrapper"><div class="row" id="image_atc_wrapper"><div class="col-xl-4 order-xl-first" id="image_wrapper"><article class="product-data-wrapper"> <div class="image-slider-container"> <div class="product-image-lightbox tocris-image" data-brand="tocris"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="https://resources.bio-techne.com/images/products/structures/janelia-fluor-525-se_6296.png" alt="Yellow dye (Reactive group: NHS ester). Applications: super resolution microscopy, confocal and live cell imaging" title="Janelia Fluor 525, SE" data-caption="Yellow dye (Reactive group: NHS ester). Applications: super resolution microscopy, confocal and live cell imaging" data-badges="" data-application="N/A" width="250" height="250" fetchpriority=high as=image class="search_result_thumbnail_image" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </article> </div> <div class="col-xl-8" id="atc_wrapper"><article class="product-data-wrapper"> <div class="atc-container"> <div class="atc-block" data-type="ds" data-blockid="ProductCart" > <form action="/commerce/addtocart" method="post" class="add-to-cart-block" data-productcode="6296"> <input type="hidden" name="print-quote" class="print-quote-input" value=""> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-3 font-weight-bold p-0">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-4 p-0 font-weight-bold d-none d-md-block">Availability</div> <div class="commerce-atc-price atc-head col-4 col-md-3 p-0 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-4 col-3 col-md-2 text-right font-weight-bold p-0">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart" class="atc-lines"> <div class="commerce-atc-ds-productline productline col-four row my-2 mx-0 text-left" data-catalog="6296/2" data-hidden=""> <div class="commerce-atc-catnum col-3 col-md-3 pl-0 m-0">6296/2</div> <div class="commerce-atc-availability col-md-4 pl-0 m-0 d-none d-md-block"> </div> <div class="commerce-atc-price col-4 col-md-3 pl-0 m-0"> </div> <div class="commerce-atc-quantity col-4 col-md-2 text-right px-0 m-0"> </div> </div> </div> <div class="atc-control ProductCart-control text-right"> <div class="row container d-flex justify-content-end mx-0 mb-3 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="" class="atc-print-quote-button btn btn-secondary m-1 col-xs-4 btn-md-block w-100">Print Quote</a> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=6296" class="atc-bulk-button btn btn-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </article> </div> </div> <div class="row datapoint_list border-top" id="key_details_wrapper"><div class="col-xl-12 mt-2" id="key_details_col_wrapper"></div> </div> </div> <div class="col-xl-3 mt-2" id="orderinfo_guarantee_wrapper"><div id="order_information_wrapper"><div id="product_shipping_block_wrapper" data-brand="tocris" data-shipping="" data-source="ep-data" data-sub_category_id="75"> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"><span class="sr-only">Loading...</span></div> </div> </div> <div id="video-tile-wrapper"><article class="product-data-wrapper"> <div class="video_tile_wrapper"> <article> <div> <lite-youtube videoid="J-YqGoL3m_c" style="background-image: url('https://resources.tocris.com/images/webinars/about-tocris-video.jpg');"> <a href="https://www.youtube.com/embed/J-YqGoL3m_c?autoplay=1" class="lty-playbtn" title="Introduction to Tocris"> <span class="lyt-visually-hidden">Introduction to Tocris</span> </a> </lite-youtube> </div> </article> </div> </article> </div> <div id="product_ads_wrapper"><article class="product-data-wrapper"> </article> </div> </div> </div> <div class="datapoint_list" id="key_details"><h3>Key Product Details</h3><table class=" table" id="key_details_table_wrapper"><tr class="product-data"> <td class="product-data-label py-2 align-top text-md-left">Description: </td> <td class="product-data-value py-2 align-top w-75 text-break"> Yellow dye (Reactive group: NHS ester). Application: confocal microscopy, super resolution microscopy (SRM) including dSTORM. Suitable for live cell imaging </td> </tr> <tr class="product-data"> <td class="product-data-label py-2 align-top text-md-left">Alternative Names: </td> <td class="product-data-value py-2 align-top w-75 text-break"> JF 525,NHS </td> </tr> <tr class="product-data"> <td class="product-data-label py-2 align-top text-md-left">Chemical Name: </td> <td class="product-data-value py-2 align-top w-75 text-break"> 3,6-Di-1-(3,3-difluoroazetidinyl)-9-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]xanthylium, inner salt </td> </tr> <tr class="product-data"> <td class="product-data-label py-2 align-top text-md-left">Molecular Weight: </td> <td class="product-data-value py-2 align-top w-75 text-break"> 623.51 </td> </tr> </table> </div> <div id="view_technical_detail_links"><div class="text-center"> <a id="view_technical" href="#technical-data-tab-tab">View Full Technical Details for Janelia Fluor<sup>®</sup> 525, NHS ester</a> </div> </div> </div> <div class="tab-content"><div id="about-data-tab-tab"></div> <ul class="nav nav-tabs nav-fill " id="productTab" role="tablist"> <li class="nav-item"> <a class="nav-link active" id="technical-data-tab-tab" href="#technical-data-tab" data-toggle="tab" role="tab" aria-controls="region-2411584697015" aria-selected="true" >Technical Data</a> </li> <li class="nav-item"> <a class="nav-link" id="citations-tab-tab" href="#citations-tab" data-toggle="tab" role="tab" aria-controls="region-8061584697120" aria-selected="false" >Citations</a> </li> </ul> <div class="tab-content rounded-bottom border-left border-right border-bottom mb-3" id="ProductTabContent"><div class="tab-pane fade show active mx-4" id="technical-data-tab" data-tab-label="Technical Data" role="tabpanel" aria-labelledby="technical-data-tab-tab"><div class="product_details_anchors"><div class="product-pages-anchors nav mx-0 pt-4 pb-2 m___nav-anchors d-none d-lg-flex"><li id="biological-activity_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor active" href="#biological-activity" id="biological-activity_li" >Biological Activity</a> </li> <li id="spectra-viewer_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#spectra-viewer" id="spectra-viewer_li" >Spectra Viewer</a> </li> <li id="technical-data_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#technical-data" id="technical-data_li" >Technical Data</a> </li> <li id="solubility_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#solubility" id="solubility_li" >Solubility</a> </li> <li id="preparing-stock-solutions_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#preparing-stock-solutions" id="preparing-stock-solutions_li" >Preparing Stock Solutions</a> </li> <li id="calculators_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#calculators" id="calculators_li" >Calculators</a> </li> <li id="references_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#references" id="references_li" >References</a> </li> <li id="product-documents_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 product-documents datasheet_static_link" href="#product-documents" id="product-documents_li" >Product Datasheets</a> </li> </div> <div id="biological-activity" class="nav-link px-0" data-anchor-label="Biological Activity"><div id="biological_activity_wrapper"><h3>Biological Activity for Janelia Fluor<sup>®</sup> 525, NHS ester</h3><div class="product-data-wrapper"> <p> <strong>Key information:</strong> Janelia Fluor<sup>®</sup> 525, NHS ester is a yellow fluorescent dye; supplied with an NHS ester reactive for the labeling of primary amines. Suitable for live cell imaging.<p><strong>Application: </strong>Suitable for confocal microscopy and super resolution microscopy (SRM) techniques, including dSTORM (in both live and fixed cells). Janelia Fluor<sup>®</sup> 525, NHS ester is cell permeable.</p><p><strong>Properties and Photophysical Data:</strong> NHS ester can be converted to relevant substrate for use in self-labeling tag systems, <em>e.g.</em> HaloTag<sup>®</sup> and SNAP-tag<sup>®</sup>. Can be multiplexed for two color imaging with <a href="/p/fluorescent-probes-dyes/janelia-fluor-635-se_6419" target="_self">Janelia Fluor<sup>®</sup> 635 NHS ester</a> (Cat. No. 6419). Excitation and emission maxima (λ) are 525 nm and 549 nm, respectively; quantum yield = 0.91; extinction coefficient = 122,000 M<sup>-1</sup>cm<sup>-1</sup>; A280 correction factor = 0.185; lactone-zwitterion equilibrium constant (K<sub>L-Z</sub>) = 0.068.</p><p>Please see the <a href="https://resources.tocris.com/pdfs/protocols/conjugation-protocol-for-amine-reactive-dyes.pdf" title="Download Conjugation Protocol for Amine Reactive Dyes" target="_blank" class="a___link-external">protocol</a> for further information on protein/antibody labeling and conjugation.</p><p><em>We also offer <a href="https://www.bio-techne.com/services/custom-antibody-services" target="_blank" class="a___link-external">Janelia Fluor<sup>®</sup> conjugated antibodies and custom conjugation services</a>.</em></p><p><em>HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.</em></p><p><em>Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.</em></p><p><em>This product has been recently renamed. The previous name for this product was Janelia Fluor<sup>®</sup> 525, SE.</em></p> </p> </div> </div> <div id="licensing_caveat_wrapper"><h3>Licensing Information</h3><div class="product-data-wrapper"> <p> Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus </p> </div> </div> <div id="optical_data_wrapper"><h3>Optical Data for Janelia Fluor<sup>®</sup> 525, NHS ester</h3> <div class="row"> <div class="col-lg-6"> <div class="product-image-lightbox"> <div class="text-center item active"> <img alt="Janelia Fluor® 525, NHS ester Dye Spectra" class="dye-chart" src="https://resources.bio-techne.com/images/products/spectra-charts/janelia-fluor-525-se-6296-spectra.jpg" title="Janelia Fluor® 525, NHS ester Dye Spectra"> </div> </div> </div> <div class="col-lg-6"> <table class="dye-table table table-striped table-condensed table-responsive"> <tbody> <tr> <th>Emission Color</th> <td>Yellow</td> </tr> <tr> <th>Brightness</th> <td> <div class="brightness border border-dark" style="background-color:#FFFF00"></div> <div class="brightness border border-dark" style="background-color:#FFFF00"></div> <div class="brightness border border-dark" style="background-color:#FFFF00"></div> <div class="brightness border border-dark" style="background-color:#FFFF00"></div> <div class="brightness border border-dark" style="background-color:#FFFF00"></div> </td> </tr> <tr> <th>λ<sub>abs</sub></th> <td>525 nm</td> </tr> <tr> <th>λ<sub>em</sub></th> <td>549 nm</td> </tr> <tr> <th>Extinction Coefficient (ε)</th> <td>122,000 M<sup>-1</sup>cm<sup>-1</sup></td> </tr> <tr> <th>Quantum Yield (φ)</th> <td>0.91</td> </tr> <tr> <th>Closest Laser line</th> <td>488 nm</td> </tr> <tr> <th>Reactive Group</th> <td>NHS ester</td> </tr> <tr> <th>Reactivity</th> <td>Primary amines</td> </tr> <tr> <th>Cell Permeable</th> <td>Yes</td> </tr> <tr> <th>Correction Factor 280</th> <td>0.185</td> </tr> <tr> <th>Application</th> <td>Confocal Microscopy, Super Resolution Microscopy (SRM)</td> </tr> <tr> <th>Alternative For</th> <td>Alexa Fluor<sup>®</sup> 532, Alexa Fluor<sup>®</sup> 514, Atto 532, CF514, CF532</td> </tr> </tbody> </table> </div> </div> <div class="row"> <div class="col-md-12"> <p id="alexa-fluor-disclaimer"> Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. </p> </div> </div> </div> </div> <div id="spectra-viewer" class="nav-link px-0" data-anchor-label="Spectra Viewer"><div class="top_region pr-0" id="spectra_viewer_wrapper"> <div class="row"> <div class="col-xs-12 col-md-7"> <h3>Plan Your Experiments</h3> <p>Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.</p> <a href="/resources/spectra-viewer" class="btn btn-primary" title="Use our spectra viewer to interactively plan your experiments."> Spectra Viewer </a> <div class="visible-xs visible-sm"><br></div> </div> <div class="col-xs-12 col-md-4"> <a href='/resources/spectra-viewer' title='Use our spectra viewer to interactively plan your experiments.'> <img src="https://resources.bio-techne.com/bio-techne-assets/images/services/spectral-viewer.png" alt='Spectra Viewer'> </a> </div> </div> </div> </div> <div id="technical-data" class="nav-link px-0" data-anchor-label="Technical Data"><div id="technical_data_wrapper"><h3>Technical Data for Janelia Fluor<sup>®</sup> 525, NHS ester</h3><table class="tablesaw tablesaw-stack table-bordered table" id="technical_data_product-data-wrapper"><tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">M. Wt</td> <td class="product-data-value p-3 px-4 text-break"> 623.51 </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Formula</td> <td class="product-data-value p-3 px-4 text-break"> C<sub>31</sub>H<sub>21</sub>F<sub>4</sub>N<sub>3</sub>O<sub>7</sub> </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Storage</td> <td class="product-data-value p-3 px-4 text-break"> Store at -20°C </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">CAS Number</td> <td class="product-data-value p-3 px-4 text-break"> 2975167-21-4 </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">PubChem ID</td> <td class="product-data-value p-3 px-4 text-break"> 134160239 </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">InChi Key</td> <td class="product-data-value p-3 px-4 text-break"> VPHPPEABRFKVDC-UHFFFAOYSA-N </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Smiles</td> <td class="product-data-value p-3 px-4 text-break"> O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CC(F)(F)C4)C=C3O5)=C(C=C/6)C5=CC6=[N+]7CC(F)(F)C\7)=C2 </td> </tr> </table> <p class="small">The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.</p> <p class="small">Tocris products are intended for laboratory research use only, unless stated otherwise.</p> </div> </div> <div id="solubility" class="nav-link px-0" data-anchor-label="Solubility"><div id="solubility"><h3>Solubility for Janelia Fluor<sup>®</sup> 525, NHS ester</h3> <table class="tablesaw tablesaw-stack table-bordered"> <thead> <tr> <th scope="col" data-tablesaw-priority="persist"></th> <th scope="col" data-tablesaw-priority="persist">Solvent</th> <th scope="col" data-tablesaw-priority="persist">Max Conc. mg/mL</th> <th scope="col" data-tablesaw-priority="persist">Max Conc. mM</th> </tr> </thead> <tbody> <tr> <th rowspan="2">Solubility</th> </tr> <tr> <td>DMSO</td> <td>31.18</td> <td> 50 </td> </tr> </tbody> </table> </div> </div> <div id="preparing-stock-solutions" class="nav-link px-0" data-anchor-label="Preparing Stock Solutions"><div id="stock_solutions_wrapper"><h3>Preparing Stock Solutions for Janelia Fluor<sup>®</sup> 525, NHS ester</h3> <p>The following data is based on the <span id="molecular_weight_type">product</span> molecular weight <span id="molecular_weight">623.51</span>.<p> <p>Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.</p> <form id="prepare_stock_solution_form" class="form-inline"> <input type="hidden" name="max_concentration" id="max_concentration_value" value="50"> <div class="d-flex mb-3"> <div> <p class="mt-2 mr-2">Select a batch to recalculate based on the batch molecular weight:</p> </div> <div> <select id="select_stock_solutions" class="form-control input-sm" name="batch"> <option value="623.51"></option> <option value="623.51">4</option> <option value="623.51">3</option> <option value="623.51">2</option> <option value="623.51">1</option> </select> <button class="btn btn-primary py-2" id="recalc_stock_soln_data">Recalculate</button> </div> </div> </form> <table class="tablesaw tablesaw-stack table-bordered"> <thead> <tr> <th scope="col" data-tablesaw-priority="persist">Concentration / Solvent Volume / Mass</th> <th scope="col" data-tablesaw-priority="persist">1 mg</th> <th scope="col" data-tablesaw-priority="persist">5 mg</th> <th scope="col" data-tablesaw-priority="persist">10 mg</th> </tr> </thead> <tbody> <tr> <td>0.5 mM</td> <td id="ss_0_5_1">3.21 mL</td> <td id="ss_0_5_5">16.04 mL</td> <td id="ss_0_5_10">32.08 mL</td> </tr> <tr> <td>2.5 mM</td> <td id="ss_2_5_1">0.64 mL</td> <td id="ss_2_5_5">3.21 mL</td> <td id="ss_2_5_10">6.42 mL</td> </tr> <tr> <td>5 mM</td> <td id="ss_5_1">0.32 mL</td> <td id="ss_5_5">1.60 mL</td> <td id="ss_5_10">3.21 mL</td> </tr> <tr> <td>25 mM</td> <td id="ss_25_1">0.06 mL</td> <td id="ss_25_5">0.32 mL</td> <td id="ss_25_10">0.64 mL</td> </tr> </tbody> </table> </div> </div> <div id="calculators" class="nav-link px-0" data-anchor-label="Calculators"><div class="calculator card p-3" id="molarity_calculator_wrapper"><h3>Molarity Calculator</h3><article class="product-data-wrapper"> <div class="product-calculator"> <p>Calculate the mass, volume, or concentration required for a solution.</p> <form id="molarity-calculator" class="calculator-wrapper"> <div class="form-inline"> <div class="calc_section"> <label>Mass</label> <div class="form-row"> <fieldset class="js-form-item form-item js-form-wrapper form-wrapper"> <div class="fieldset-wrapper"> <div class="float-left mx-1 js-form-item mb-2 form-item js-form-type-textfield form-item-molarity-calculator-mass js-form-item-molarity-calculator-mass"> <input id="molarity_calculator_mass" class="calculator_input calculator_text form-control form-text" placeholder="mass" type="text" name="molarity_calculator_mass" value="" size="10" maxlength="6"> </div> <div class="float-left mx-1 js-form-item mb-2 form-item js-form-type-select form-item-molarity-calculator-mass-unit js-form-item-molarity-calculator-mass-unit"> <select id="molarity_calculator_mass_unit" class="calculator_select custom-select form-select" name="molarity_calculator_mass_unit"> <option value="-12">pg</option> <option value="-9">ng</option> <option value="-6">µg</option> <option value="-3" selected="selected">mg</option> <option value="0">g</option> <option value="3">kg</option> </select> </div> </div> </fieldset> </div> </div> <div class="calculator_equals calculator_operator mx-2 position-relative">=</div> <div class="calc_section"> <label>Concentration</label> <div class="form-row"> <fieldset class="js-form-item form-item js-form-wrapper form-wrapper"> <div class="fieldset-wrapper"> <div class="float-left mx-1 js-form-item mb-2 form-item js-form-type-textfield form-item-molarity-calculator-concentration js-form-item-molarity-calculator-concentration"> <input id="molarity_calculator_concentration" placeholder="conc." class="calculator_input calculator_text form-control form-text" type="text" name="molarity_calculator_concentration" value="" size="10" maxlength="6"> </div> <div class="float-left mx-1 js-form-item mb-2 form-item js-form-type-select form-item-molarity-calculator-concentration-unit js-form-item-molarity-calculator-concentration-unit"> <select id="molarity_calculator_concentration_unit" class="calculator_select custom-select form-select" name="molarity_calculator_concentration_unit"> <option value="-15">fM</option> <option value="-12">pM</option> <option value="-9">nM</option> <option value="-6">µM</option> <option value="-3" selected="selected">mM</option> <option value="0">M</option> </select> </div> </div> </fieldset> </div> </div> <div class="calculator_multiply calculator_operator mx-2 position-relative">x</div> <div class="calc_section"> <label>Volume</label> <div class="form-row"> <fieldset class="js-form-item form-item js-form-wrapper form-wrapper"> <div class="fieldset-wrapper"> <div class="float-left mx-1 js-form-item mb-2 form-item js-form-type-textfield form-item-molarity-calculator-volume js-form-item-molarity-calculator-volume"> <input id="molarity_calculator_volume" placeholder="volume" class="calculator_input calculator_text form-control form-text" type="text" name="molarity_calculator_volume" value="" size="10" maxlength="6"> </div> <div class="float-left mx-1 js-form-item mb-2 form-item js-form-type-select form-item-molarity-calculator-volume-unit js-form-item-molarity-calculator-volume-unit"> <select id="molarity_calculator_volume_unit" class="calculator_select custom-select form-select" name="molarity_calculator_volume_unit"> <option value="-9">nL</option> <option value="-6">µL</option> <option value="-3" selected="selected">mL</option> <option value="0">L</option> </select> </div> </div> </fieldset> </div> </div> <div class="calculator_multiply calculator_operator mx-2 position-relative">x</div> <div class="calc_section"> <label>M. Wt*</label> <div class="form-row"> <fieldset class="js-form-item form-item js-form-wrapper form-wrapper"> <div class="fieldset-wrapper"> <div class="float-left mx-1 js-form-item mb-2 form-item js-form-type-textfield form-item-molarity-calculator-molecular-weight js-form-item-molarity-calculator-molecular-weight"> <input id="molarity_calculator_molecular_weight" placeholder="mwt" class="calculator_input calculator_text form-control form-text" type="text" name="molarity_calculator_molecular_weight" value="623.51" size="10" maxlength="6"> </div> </div> </fieldset> <div class="calculator_operator mx-2 position-relative"> g/mol </div> </div> </div> </div> <div class="alert alert-danger calculator-error d-none" role="alert"></div> <div class="calculator_footer"> <fieldset class="js-form-item form-item js-form-wrapper form-wrapper"> <div class="fieldset-wrapper"> <input id="button_molarity_calculator_calculate" class="calculator_button btn btn-primary button js-form-submit form-submit" type="submit" name="op" value="Calculate"> </div> </fieldset> </div> <div class="calculator_disclaimer"> <p>*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).</p> </div> </form> </div> </article> </div> <div class="calculator card p-3" id="dilution_calculator_wrapper"><h3>Dilution Calculator</h3><article class="product-data-wrapper"> <div class="product-calculator"> <p>Calculate the dilution required to prepare a stock solution.</p> <form id="dilution-calculator" class="calculator-wrapper"> <div class="form-inline"> <div class="calc_section"> <label>Concentration 1 (C1)</label> <div class="form-row"> <fieldset class="js-form-item form-item js-form-wrapper form-wrapper"> <div class="fieldset-wrapper"> <div class="float-left mx-1 js-form-item mb-2 form-item js-form-type-textfield form-item-dilution-calculator-concentration js-form-item-dilution-calculator-concentration"> <input id="dilution_calculator_concentration" placeholder="conc." class="calculator_input calculator_text form-control form-text" type="text" name="dilution_calculator_concentration" value="" size="10" maxlength="6"> </div> <div class="float-left mx-1 js-form-item mb-2 form-item js-form-type-select form-item-dilution-calculator-concentration-unit js-form-item-dilution-calculator-concentration-unit"> <select id="dilution_calculator_concentration_unit" class="calculator_select custom-select form-select" name="dilution_calculator_concentration_unit"> <option value="fM">fM</option> <option value="pM">pM</option> <option value="nM">nM</option> <option value="uM">µM</option> <option value="mM" selected="selected">mM</option> <option value="M">M</option> </select> </div> </div> </fieldset> </div> </div> <div class="calculator_multiply calculator_operator mx-2 position-relative">x</div> <div class="calc_section"> <label>Volume 1 (V1)</label> <div class="form-row"> <fieldset class="js-form-item form-item js-form-wrapper form-wrapper"> <div class="fieldset-wrapper"> <div class="float-left mx-1 js-form-item mb-2 form-item js-form-type-textfield form-item-dilution-calculator-volume js-form-item-dilution-calculator-volume"> <input id="dilution_calculator_volume" placeholder="volume" class="calculator_input calculator_text form-control form-text" type="text" name="dilution_calculator_volume" value="" size="10" maxlength="6"> </div> <div class="float-left mx-1 js-form-item mb-2 form-item js-form-type-select form-item-dilution-calculator-volume-unit js-form-item-dilution-calculator-volume-unit"> <select id="dilution_calculator_volume_unit" class="calculator_select custom-select form-select" name="dilution_calculator_volume_unit"> <option value="nl">nL</option> <option value="ul">µL</option> <option value="ml" selected="selected">mL</option> <option value="l">L</option> </select> </div> </div> </fieldset> </div> </div> <div class="calculator_equals calculator_operator mx-2 position-relative">=</div> <div class="calc_section"> <label>Concentration 2 (C2)</label> <div class="form-row"> <fieldset class="js-form-item form-item js-form-wrapper form-wrapper"> <div class="fieldset-wrapper"> <div class="float-left mx-1 js-form-item mb-2 form-item js-form-type-textfield form-item-dilution-calculator-concentration2 js-form-item-dilution-calculator-concentration2"> <input id="dilution_calculator_concentration2" placeholder="conc." class="calculator_input calculator_text form-control form-text" type="text" name="dilution_calculator_concentration2" value="" size="10" maxlength="6"> </div> <div class="float-left mx-1 js-form-item mb-2 form-item js-form-type-select form-item-dilution-calculator-concentration2-unit js-form-item-dilution-calculator-concentration2-unit"> <select id="dilution_calculator_concentration2_unit" class="calculator_select custom-select form-select" name="dilution_calculator_concentration2_unit"> <option value="fM">fM</option> <option value="pM">pM</option> <option value="nM">nM</option> <option value="uM">µM</option> <option value="mM" selected="selected">mM</option> <option value="M">M</option> </select> </div> </div> </fieldset> </div> </div> <div class="calculator_multiply calculator_operator mx-2 position-relative">x</div> <div class="calc_section"> <label>Volume 2 (V2)</label> <div class="form-row"> <fieldset class="js-form-item form-item js-form-wrapper form-wrapper"> <div class="fieldset-wrapper"> <div class="float-left mx-1 js-form-item mb-2 form-item js-form-type-textfield form-item-dilution-calculator-volume2 js-form-item-dilution-calculator-volume2"> <input id="dilution_calculator_volume2" placeholder="volume" class="calculator_input calculator_text form-control form-text" type="text" name="dilution_calculator_volume2" value="" size="10" maxlength="6"> </div> <div class="float-left mx-1 js-form-item mb-2 form-item js-form-type-select form-item-dilution-calculator-volume2-unit js-form-item-dilution-calculator-volume2-unit"> <select id="dilution_calculator_volume2_unit" class="calculator_select custom-select form-select" name="dilution_calculator_volume2_unit"> <option value="nl">nL</option> <option value="ul">µL</option> <option value="ml" selected="selected">mL</option> <option value="l">L</option> </select> </div> </div> </fieldset> </div> </div> </div> <div class="alert alert-danger calculator-error d-none" role="alert"></div> <div class="calculator_footer"> <fieldset class="js-form-item form-item js-form-wrapper form-wrapper"> <div class="fieldset-wrapper"> <input id="button_dilution_calculator_calculate" class="calculator_button btn btn-primary button js-form-submit form-submit" type="submit" name="op" value="Calculate"> </div> </fieldset> </div> </form> </div> </article> </div> <div class="calculator card p-3" id="reconstitution_calculator_wrapper"><h3>Reconstitution Calculator</h3><article class="product-data-wrapper"> <div class="product-calculator"> <form id="reconstitution-calculator" class="calculator-wrapper"> <p>The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.</p> <div class="form-inline"> <div class="calc_section"> <label>Volume (to add to vial)</label> <div class="form-row"> <fieldset class="js-form-item form-item js-form-wrapper form-wrapper"> <div class="fieldset-wrapper"> <div class="mx-1 p-0 js-form-item mb-2 form-item js-form-type-textfield form-item-reconstitution-calculator-volume js-form-item-reconstitution-calculator-volume"> <input id="reconstitution_calculator_volume" placeholder="volume" class="calculator_input calculator_text form-control form-text" type="text" name="reconstitution_calculator_volume" value="" size="10" maxlength="6"> </div> <div class="mx-1 p-0 js-form-item mb-2 form-item js-form-type-select form-item-reconstitution-calculator-volume-unit js-form-item-reconstitution-calculator-volume-unit"> <select id="reconstitution_calculator_volume_unit" class="calculator_select custom-select form-select" name="reconstitution_calculator_volume_unit"> <option value="ml">ml</option> <option value="ul" selected="selected">µl</option> </select> </div> </div> </fieldset> </div> </div> <div class="calculator_equals calculator_operator mx-2 position-relative">=</div> <div class="calc_section"> <label>Mass (in vial)</label> <div class="form-row"> <fieldset class="js-form-item form-item js-form-wrapper form-wrapper"> <div class="fieldset-wrapper"> <div class="mx-1 p-0 js-form-item mb-2 form-item js-form-type-textfield form-item-reconstitution-calculator-mass js-form-item-reconstitution-calculator-mass"> <input id="reconstitution_calculator_mass" class="calculator_input calculator_text form-control form-text" placeholder="mass" type="text" name="reconstitution_calculator_mass" value="" size="10" maxlength="6"> </div> <div class="mx-1 p-0 js-form-item mb-2 form-item js-form-type-select form-item-reconstitution-calculator-mass-unit js-form-item-reconstitution-calculator-mass-unit"> <select id="reconstitution_calculator_mass_unit" class="calculator_select custom-select form-select" name="reconstitution_calculator_mass_unit"> <option value="mg">mg</option> <option value="ug" selected="selected">ug</option> <option value="ng">ng</option> </select> </div> </div> </fieldset> </div> </div> <div class="calculator_divide_by calculator_operator mx-2 position-relative">÷</div> <div class="calc_section"> <div> <label>Desired Reconstitution Concentration</label> </div> <div class="form-row"> <fieldset class="js-form-item form-item js-form-wrapper form-wrapper"> <div class="fieldset-wrapper"> <div class="mx-1 p-0 js-form-item mb-2 form-item js-form-type-textfield form-item-reconstitution-calculator-concentration js-form-item-reconstitution-calculator-concentration"> <input id="reconstitution_calculator_concentration" placeholder="conc." class="calculator_input calculator_text form-control form-text" type="text" name="reconstitution_calculator_concentration" value="" size="10" maxlength="6"> </div> <div class="mx-1 p-0 js-form-item mb-2 form-item js-form-type-select form-item-reconstitution-calculator-concentration-unit js-form-item-reconstitution-calculator-concentration-unit"> <select id="reconstitution_calculator_concentration_unit" class="calculator_select custom-select form-select" name="reconstitution_calculator_concentration_unit"> <option value="mg/ml">mg/ml</option> <option value="ug/ml" selected="selected">µg/ml</option> <option value="ng/ml">ng/ml</option> </select> </div> </div> </fieldset> </div> </div> </div> <div class="alert alert-danger calculator-error d-none" role="alert"></div> <div class="calculator_footer"> <fieldset class="js-form-item form-item js-form-wrapper form-wrapper"> <div class="fieldset-wrapper"> <input id="button_reconstitution_calculator_calculate" class="calculator_button btn btn-primary button js-form-submit form-submit" type="submit" name="op" value="Calculate"> </div> </fieldset> </div> </form> </div> </article> </div> </div> <div id="references" class="nav-link px-0" data-anchor-label="References"> <div id="references"> <h3>References for Janelia Fluor<sup>®</sup> 525, NHS ester</h3> <p>References are publications that support the biological activity of the product.</p> <ul> <li> <strong>Zheng</strong> <a href="https://www.ncbi.nlm.nih.gov/pubmed/31572787" target="_blank" rel="nofollow">Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging.</a> ACS Cent.Sci. 2019 PMID: 31572787 </li> <li> <strong>Grimm</strong> <a href="https://www.ncbi.nlm.nih.gov/pubmed/28869757" target="_blank" rel="nofollow">A general method to fine-tune fluorophores for live-cell and<em> in vivo</em> imaging.</a> Nat. Methods 2017 PMID: 28869757 </li> </ul> </div> </div> <div id="product-datasheets-anchor" class="nav-link static-anchor p-0" data-anchor-label="Product Datasheets"></div> </div> </div> <div class="tab-pane fade mx-4 py-4" id="citations-tab" data-tab-label="Citations" role="tabpanel" aria-labelledby="citations-tab-tab"><div class="product_details_anchors mb-2"> <div class="nav mx-0 pt-4 pb-2 m___nav-anchors d-none d-lg-flex"> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#citations"> Citations </a> </li> </div> </div> <div id="citations"> <section id="citations-tab-content-placeholder" class="tab-content-placeholder" data-id="citations" data-product_code="6296"> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> </section> </div> </div> </div> </div> <div id="product-documents"> <div id="datasheet-accordion" class="accordion border mb-4"> <div class="rounded-top bg-white"> <h3 class="m-0 p-3">Product Documents for Janelia Fluor<sup>®</sup> 525, NHS ester</h3> </div> <div class="card-header"> <a data-toggle="collapse" data-target="#collapse_one" aria-expanded="false" aria-controls="collapse_one"> <h5 class="mb-0"> COA </h5> </a> </div> <div id="collapse_one" class="collapse show " aria-labelledby="heading_one" data-parent="#datasheet-accordion"> <div class="card-body"> <div class="row"> <div class="col-12"> <h2>Certificate of Analysis</h2> <p>To download a Certificate of Analysis, please select a batch below.</p> </div> </div> <section class="row"> <div class="col-md-6"> <form class="form-inline"> <div class="form-group select_sds_batch"> <div class="select_wrapper"> <select class="form-control mr-2" name="coa" id="tocris_coa_select"> <option value="">Select Your Batch</option> <option value="6296_4_coa.pdf">4</option> <option value="6296_3_coa.pdf">3</option> <option value="6296_2_coa.pdf">2</option> <option value="6296_1_coa.pdf">1</option> </select> </div> <button class="btn btn-primary" id="tocris_coa_view_batch"> View Batch </button> </div> </form> </div> <div id="pdf-result" class="col-md-6 mt-2"> <div class="mt-3" id="coa-result"> </div> </div> </section> </div> </div> <div class="card-header"> <a class="collapsed" data-toggle="collapse" data-target="#collapse_three" aria-expanded="false" aria-controls="collapse_three" > <h5 class="mb-0"> SDS </h5> </a> </div> <div id="collapse_three" class="collapse rounded-bottom" aria-labelledby="heading_three" data-parent="#datasheet-accordion"> <div class="card-body"> <div class="row sds-row"> <div class="col text-bt-blue nowrap mt-3" id="sds-tocris-wrap"> <button id="sds_link" class="sds-link datasheet_link datasheet-button-link" data-uri="/sds-pdf-tocris?pdf=6296_sds.pdf"> Download SDS </button> </div> <div class="col-md-5 sds-pdf-result datasheet-loading"></div> </div> </div> </div> </div> </div> <div id="product-page-details" data-time-Total="0" data-time-ToCompile="0" data-time-ToBuild="0" 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