<?xml version="1.0" encoding="UTF-8"?> <article key="pdf/6057" mdate="2012-06-28 00:00:00"> <author>Rungsinee Phongpradist and Sawitree Chiampanichayakul and Singkome Tima and Teruna J. Siahaan and Cory J. Berkland and Songyot Anuchapreeda and Chadarat Ampasavate</author> <title>Potential cIBRConjugated PLGA Nanoparticles for Selective Targeting to Leukemic Cells</title> <pages>234 - 242</pages> <year>2012</year> <volume>6</volume> <number>6</number> <journal>International Journal of Pharmacological and Pharmaceutical Sciences</journal> <ee>https://publications.waset.org/pdf/6057</ee> <url>https://publications.waset.org/vol/66</url> <publisher>World Academy of Science, Engineering and Technology</publisher> <abstract>The expression of LFA1 diverges from the physiological condition, thus active targeting carrier can provide the benefits from difference into LFA1 expression in various conditions. Here, the selectivity of cIBRconjugated nanoparticles (cIBRNPs), in terms of uptake, was investigated using PBMCs, Mixed PBMCMolt 3 cells and Molt3 cells. The expressions of LFA1 on Molt3 cells, from flow cytometry and Western blot, possessed the highest level whereas PBMCs showed the lowest level. The kinetic uptake profiles of cIBRNPs were obtained by flow cytometry, which the degree of cellular uptake presented a similar trend with the level of LFA1 indicating the influence of LFA1 expression on the cellular uptake of cIBRNPs. The conformation of LFA1 had a slight effect on the cellular uptake of cIBRNPs. Overall we demonstrated that cIBRNPs enhanced cellular uptake and improved the selectivity of drug carriers to LFA1 on the leukemia cells, which related with the order of LFA1 expression.</abstract> <index>Open Science Index 66, 2012</index> </article>