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li > ul.mfn-megamenu > li.hide-border{border-right-width:0}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li a{border-bottom-width:0;padding:9px 15px;line-height:120%}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{font-weight:700}.rtl .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child{border-right-width:0}.rtl .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:last-child{border-right-width:1px}body.header-shop #Top_bar #menu{display:flex!important;background-color:transparent}.header-shop #Top_bar.is-sticky .top_bar_row_second{display:none}.header-plain:not(.menuo-right) #Header .top_bar_left{width:auto!important}.header-stack.header-center #Top_bar #menu{display:inline-block!important}.header-simple #Top_bar #menu{display:none;height:auto;width:300px;bottom:auto;top:100%;right:1px;position:absolute;margin:0}.header-simple #Header a.responsive-menu-toggle{display:block;right:10px}.header-simple #Top_bar #menu > ul{width:100%;float:left}.header-simple #Top_bar #menu ul li{width:100%;padding-bottom:0;border-right:0;position:relative}.header-simple #Top_bar #menu ul li a{padding:0 20px;margin:0;display:block;height:auto;line-height:normal;border:none}.header-simple #Top_bar #menu ul li a:not(.menu-toggle):after{display:none}.header-simple #Top_bar #menu ul li a span{border:none;line-height:44px;display:inline;padding:0}.header-simple #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:44px;height:44px;line-height:44px;font-size:30px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33;transform:unset}.header-simple #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+";position:static}.header-simple #Top_bar #menu ul li.hover > .menu-toggle:after{content:"-"}.header-simple #Top_bar #menu ul li.hover a{border-bottom:0}.header-simple #Top_bar #menu ul.mfn-megamenu li .menu-toggle{display:none}.header-simple #Top_bar #menu ul li ul{position:relative!important;left:0!important;top:0;padding:0;margin:0!important;width:auto!important;background-image:none}.header-simple #Top_bar #menu ul li ul li{width:100%!important;display:block;padding:0}.header-simple #Top_bar #menu ul li ul li a{padding:0 20px 0 30px}.header-simple #Top_bar #menu ul li ul li a .menu-arrow{display:none}.header-simple #Top_bar #menu ul li ul li a span{padding:0}.header-simple #Top_bar #menu ul li ul li a span:after{display:none!important}.header-simple #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{text-transform:uppercase;font-weight:400}.header-simple #Top_bar .menu > li > ul.mfn-megamenu > li > ul{display:block!important;position:inherit;left:auto;top:auto}.header-simple #Top_bar #menu ul li ul li ul{border-left:0!important;padding:0;top:0}.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 20px 0 40px}.rtl.header-simple #Top_bar #menu{left:1px;right:auto}.rtl.header-simple #Top_bar a.responsive-menu-toggle{left:10px;right:auto}.rtl.header-simple #Top_bar #menu ul li.submenu .menu-toggle{left:0;right:auto}.rtl.header-simple #Top_bar #menu ul li ul{left:auto!important;right:0!important}.rtl.header-simple #Top_bar #menu ul li ul li a{padding:0 30px 0 20px}.rtl.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 40px 0 20px}.menu-highlight #Top_bar .menu > li{margin:0 2px}.menu-highlight:not(.header-creative) #Top_bar .menu > li > a{padding:0;-webkit-border-radius:5px;border-radius:5px}.menu-highlight #Top_bar .menu > li > a:after{display:none}.menu-highlight #Top_bar .menu > li > a span:not(.description){line-height:50px}.menu-highlight #Top_bar .menu > li > a span.description{display:none}.menu-highlight.header-stack #Top_bar .menu > li > a{margin:10px 0!important}.menu-highlight.header-stack #Top_bar .menu > li > a span:not(.description){line-height:40px}.menu-highlight.header-simple #Top_bar #menu ul li,.menu-highlight.header-creative #Top_bar #menu ul li{margin:0}.menu-highlight.header-simple #Top_bar #menu ul li > a,.menu-highlight.header-creative #Top_bar #menu ul li > a{-webkit-border-radius:0;border-radius:0}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a{margin:10px 0!important;padding:5px 0!important}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a span{line-height:30px!important}.header-modern.menu-highlight.menuo-right .menu_wrapper{margin-right:20px}.menu-line-below #Top_bar .menu > li > a:not(.menu-toggle):after{top:auto;bottom:-4px}.menu-line-below #Top_bar.is-sticky .menu > li > a:not(.menu-toggle):after{top:auto;bottom:-4px}.menu-line-below-80 #Top_bar:not(.is-sticky) .menu > li > a:not(.menu-toggle):after{height:4px;left:10%;top:50%;margin-top:20px;width:80%}.menu-line-below-80-1 #Top_bar:not(.is-sticky) .menu > li > a:not(.menu-toggle):after{height:1px;left:10%;top:50%;margin-top:20px;width:80%}.menu-link-color #Top_bar .menu > li > a:not(.menu-toggle):after{display:none!important}.menu-arrow-top #Top_bar .menu > li > a:after{background:none repeat scroll 0 0 rgba(0,0,0,0)!important;border-color:#ccc transparent transparent;border-style:solid;border-width:7px 7px 0;display:block;height:0;left:50%;margin-left:-7px;top:0!important;width:0}.menu-arrow-top #Top_bar.is-sticky .menu > li > a:after{top:0!important}.menu-arrow-bottom #Top_bar .menu > li > a:after{background:none!important;border-color:transparent transparent #ccc;border-style:solid;border-width:0 7px 7px;display:block;height:0;left:50%;margin-left:-7px;top:auto;bottom:0;width:0}.menu-arrow-bottom #Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:0}.menuo-no-borders #Top_bar .menu > li > a span{border-width:0!important}.menuo-no-borders #Header_creative #Top_bar .menu > li > a span{border-bottom-width:0}.menuo-no-borders.header-plain #Top_bar a#header_cart,.menuo-no-borders.header-plain #Top_bar a#search_button,.menuo-no-borders.header-plain #Top_bar .wpml-languages,.menuo-no-borders.header-plain #Top_bar a.action_button{border-width:0}.menuo-right #Top_bar .menu_wrapper{float:right}.menuo-right.header-stack:not(.header-center) #Top_bar .menu_wrapper{margin-right:150px}body.header-creative{padding-left:50px}body.header-creative.header-open{padding-left:250px}body.error404,body.under-construction,body.template-blank,body.under-construction.header-rtl.header-creative.header-open{padding-left:0!important;padding-right:0!important}.header-creative.footer-fixed #Footer,.header-creative.footer-sliding #Footer,.header-creative.footer-stick #Footer.is-sticky{box-sizing:border-box;padding-left:50px}.header-open.footer-fixed #Footer,.header-open.footer-sliding #Footer,.header-creative.footer-stick #Footer.is-sticky{padding-left:250px}.header-rtl.header-creative.footer-fixed #Footer,.header-rtl.header-creative.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-left:0;padding-right:50px}.header-rtl.header-open.footer-fixed #Footer,.header-rtl.header-open.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-right:250px}#Header_creative{background-color:#fff;position:fixed;width:250px;height:100%;left:-200px;top:0;z-index:9002;-webkit-box-shadow:2px 0 4px 2px rgba(0,0,0,.15);box-shadow:2px 0 4px 2px rgba(0,0,0,.15)}#Header_creative .container{width:100%}#Header_creative .creative-wrapper{opacity:0;margin-right:50px}#Header_creative a.creative-menu-toggle{display:block;width:34px;height:34px;line-height:34px;font-size:22px;text-align:center;position:absolute;top:10px;right:8px;border-radius:3px}.admin-bar #Header_creative a.creative-menu-toggle{top:42px}#Header_creative #Top_bar{position:static;width:100%}#Header_creative #Top_bar .top_bar_left{width:100%!important;float:none}#Header_creative #Top_bar .logo{float:none;text-align:center;margin:15px 0}#Header_creative #Top_bar #menu{background-color:transparent}#Header_creative #Top_bar .menu_wrapper{float:none;margin:0 0 30px}#Header_creative #Top_bar .menu > li{width:100%;float:none;position:relative}#Header_creative #Top_bar .menu > li > a{padding:0;text-align:center}#Header_creative #Top_bar .menu > li > a:after{display:none}#Header_creative #Top_bar .menu > li > a span{border-right:0;border-bottom-width:1px;line-height:38px}#Header_creative #Top_bar .menu li ul{left:100%;right:auto;top:0;box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03);-webkit-box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03)}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu{margin:0;width:700px!important}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu > li > ul{left:0}#Header_creative #Top_bar .menu li ul li a{padding-top:9px;padding-bottom:8px}#Header_creative #Top_bar .menu li ul li ul{top:0}#Header_creative #Top_bar .menu > li > a span.description{display:block;font-size:13px;line-height:28px!important;clear:both}.menuo-arrows #Top_bar .menu > li.submenu > a > span:after{content:unset!important}#Header_creative #Top_bar .top_bar_right{width:100%!important;float:left;height:auto;margin-bottom:35px;text-align:center;padding:0 20px;top:0;-webkit-box-sizing:border-box;-moz-box-sizing:border-box;box-sizing:border-box}#Header_creative #Top_bar .top_bar_right:before{content:none}#Header_creative #Top_bar .top_bar_right .top_bar_right_wrapper{flex-wrap:wrap;justify-content:center}#Header_creative #Top_bar .top_bar_right .top-bar-right-icon,#Header_creative #Top_bar .top_bar_right .wpml-languages,#Header_creative #Top_bar .top_bar_right .top-bar-right-button,#Header_creative #Top_bar .top_bar_right .top-bar-right-input{min-height:30px;margin:5px}#Header_creative #Top_bar .search_wrapper{left:100%;top:auto}#Header_creative #Top_bar .banner_wrapper{display:block;text-align:center}#Header_creative #Top_bar .banner_wrapper img{max-width:100%;height:auto;display:inline-block}#Header_creative #Action_bar{display:none;position:absolute;bottom:0;top:auto;clear:both;padding:0 20px;box-sizing:border-box}#Header_creative #Action_bar .contact_details{width:100%;text-align:center;margin-bottom:20px}#Header_creative #Action_bar .contact_details li{padding:0}#Header_creative #Action_bar .social{float:none;text-align:center;padding:5px 0 15px}#Header_creative #Action_bar .social li{margin-bottom:2px}#Header_creative #Action_bar .social-menu{float:none;text-align:center}#Header_creative #Action_bar .social-menu li{border-color:rgba(0,0,0,.1)}#Header_creative .social li a{color:rgba(0,0,0,.5)}#Header_creative .social li a:hover{color:#000}#Header_creative .creative-social{position:absolute;bottom:10px;right:0;width:50px}#Header_creative .creative-social li{display:block;float:none;width:100%;text-align:center;margin-bottom:5px}.header-creative .fixed-nav.fixed-nav-prev{margin-left:50px}.header-creative.header-open .fixed-nav.fixed-nav-prev{margin-left:250px}.menuo-last #Header_creative #Top_bar .menu li.last ul{top:auto;bottom:0}.header-open #Header_creative{left:0}.header-open #Header_creative .creative-wrapper{opacity:1;margin:0!important}.header-open #Header_creative .creative-menu-toggle,.header-open #Header_creative .creative-social{display:none}.header-open #Header_creative #Action_bar{display:block}body.header-rtl.header-creative{padding-left:0;padding-right:50px}.header-rtl #Header_creative{left:auto;right:-200px}.header-rtl #Header_creative .creative-wrapper{margin-left:50px;margin-right:0}.header-rtl #Header_creative a.creative-menu-toggle{left:8px;right:auto}.header-rtl #Header_creative .creative-social{left:0;right:auto}.header-rtl #Footer #back_to_top.sticky{right:125px}.header-rtl #popup_contact{right:70px}.header-rtl #Header_creative #Top_bar .menu li ul{left:auto;right:100%}.header-rtl #Header_creative #Top_bar .search_wrapper{left:auto;right:100%}.header-rtl .fixed-nav.fixed-nav-prev{margin-left:0!important}.header-rtl .fixed-nav.fixed-nav-next{margin-right:50px}body.header-rtl.header-creative.header-open{padding-left:0;padding-right:250px!important}.header-rtl.header-open #Header_creative{left:auto;right:0}.header-rtl.header-open #Footer #back_to_top.sticky{right:325px}.header-rtl.header-open #popup_contact{right:270px}.header-rtl.header-open .fixed-nav.fixed-nav-next{margin-right:250px}#Header_creative.active{left:-1px}.header-rtl #Header_creative.active{left:auto;right:-1px}#Header_creative.active .creative-wrapper{opacity:1;margin:0}.header-creative .vc_row[data-vc-full-width]{padding-left:50px}.header-creative.header-open .vc_row[data-vc-full-width]{padding-left:250px}.header-open .vc_parallax .vc_parallax-inner{left:auto;width:calc(100% - 250px)}.header-open.header-rtl .vc_parallax .vc_parallax-inner{left:0;right:auto}#Header_creative.scroll{height:100%;overflow-y:auto}#Header_creative.scroll:not(.dropdown) .menu li ul{display:none!important}#Header_creative.scroll #Action_bar{position:static}#Header_creative.dropdown{outline:none}#Header_creative.dropdown #Top_bar .menu_wrapper{float:left;width:100%}#Header_creative.dropdown #Top_bar #menu ul li{position:relative;float:left}#Header_creative.dropdown #Top_bar #menu ul li a:not(.menu-toggle):after{display:none}#Header_creative.dropdown #Top_bar #menu ul li a span{line-height:38px;padding:0}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:38px;height:38px;line-height:38px;font-size:26px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33;z-index:203}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+";position:static}#Header_creative.dropdown #Top_bar #menu ul li.hover > .menu-toggle:after{content:"-"}#Header_creative.dropdown #Top_bar #menu ul.sub-menu li:not(:last-of-type) a{border-bottom:0}#Header_creative.dropdown #Top_bar #menu ul.mfn-megamenu li .menu-toggle{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul{position:relative!important;left:0!important;top:0;padding:0;margin-left:0!important;width:auto!important;background-image:none}#Header_creative.dropdown #Top_bar #menu ul li ul li{width:100%!important}#Header_creative.dropdown #Top_bar #menu ul li ul li a{padding:0 10px;text-align:center}#Header_creative.dropdown #Top_bar #menu ul li ul li a .menu-arrow{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul li a span{padding:0}#Header_creative.dropdown #Top_bar #menu ul li ul li a span:after{display:none!important}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{text-transform:uppercase;font-weight:400}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu > li > ul{display:block!important;position:inherit;left:auto;top:auto}#Header_creative.dropdown #Top_bar #menu ul li ul li ul{border-left:0!important;padding:0;top:0}#Header_creative{transition:left .5s ease-in-out,right .5s ease-in-out}#Header_creative .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out .5s}#Header_creative.active .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out}}@media only screen and (min-width:1240px){#Top_bar.is-sticky{position:fixed!important;width:100%;left:0;top:-60px;height:60px;z-index:701;background:#fff;opacity:.97;-webkit-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);-moz-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);box-shadow:0 2px 5px 0 rgba(0,0,0,0.1)}.layout-boxed.header-boxed #Top_bar.is-sticky{left:50%;-webkit-transform:translateX(-50%);transform:translateX(-50%)}#Top_bar.is-sticky .top_bar_left,#Top_bar.is-sticky .top_bar_right,#Top_bar.is-sticky .top_bar_right:before{background:none;box-shadow:unset}#Top_bar.is-sticky .logo{width:auto;margin:0 30px 0 20px;padding:0}#Top_bar.is-sticky #logo,#Top_bar.is-sticky .custom-logo-link{padding:5px 0!important;height:50px!important;line-height:50px!important}.logo-no-sticky-padding #Top_bar.is-sticky #logo{height:60px!important;line-height:60px!important}#Top_bar.is-sticky #logo img.logo-main{display:none}#Top_bar.is-sticky #logo img.logo-sticky{display:inline;max-height:35px}.logo-sticky-width-auto #Top_bar.is-sticky #logo img.logo-sticky{width:auto}#Top_bar.is-sticky .menu_wrapper{clear:none}#Top_bar.is-sticky .menu_wrapper .menu > li > a{padding:15px 0}#Top_bar.is-sticky .menu > li > a,#Top_bar.is-sticky .menu > li > a span{line-height:30px}#Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:-4px}#Top_bar.is-sticky .menu > li > a span.description{display:none}#Top_bar.is-sticky .secondary_menu_wrapper,#Top_bar.is-sticky .banner_wrapper{display:none}.header-overlay #Top_bar.is-sticky{display:none}.sticky-dark #Top_bar.is-sticky,.sticky-dark #Top_bar.is-sticky #menu{background:rgba(0,0,0,.8)}.sticky-dark #Top_bar.is-sticky .menu > li:not(.current-menu-item) > a{color:#fff}.sticky-dark #Top_bar.is-sticky .top_bar_right .top-bar-right-icon{color:rgba(255,255,255,.9)}.sticky-dark #Top_bar.is-sticky .top_bar_right .top-bar-right-icon svg .path{stroke:rgba(255,255,255,.9)}.sticky-dark #Top_bar.is-sticky .wpml-languages a.active,.sticky-dark #Top_bar.is-sticky .wpml-languages ul.wpml-lang-dropdown{background:rgba(0,0,0,0.1);border-color:rgba(0,0,0,0.1)}.sticky-white #Top_bar.is-sticky,.sticky-white #Top_bar.is-sticky #menu{background:rgba(255,255,255,.8)}.sticky-white #Top_bar.is-sticky .menu > li:not(.current-menu-item) > a{color:#222}.sticky-white #Top_bar.is-sticky .top_bar_right .top-bar-right-icon{color:rgba(0,0,0,.8)}.sticky-white #Top_bar.is-sticky .top_bar_right .top-bar-right-icon svg .path{stroke:rgba(0,0,0,.8)}.sticky-white #Top_bar.is-sticky .wpml-languages a.active,.sticky-white #Top_bar.is-sticky .wpml-languages ul.wpml-lang-dropdown{background:rgba(255,255,255,0.1);border-color:rgba(0,0,0,0.1)}}@media only screen and 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#Top_bar:not(.is-sticky){background-color:rgba(255,255,255,0.8)}}@media only screen and ( max-width:767px ){#Top_bar{background-color:#ffffff !important}#Action_bar{background-color:#007dc5 !important}#Action_bar .contact_details{color:#222222}#Action_bar .contact_details a{color:#0095eb}#Action_bar .contact_details a:hover{color:#007cc3}#Action_bar .social li a,#Action_bar .social-menu a{color:#bbbbbb!important}#Action_bar .social li a:hover,#Action_bar .social-menu a:hover{color:#777777!important}} form input.display-none{display:none!important} </style> <link rel='stylesheet' id='style-css' href='https://www.wyatt.com/wp-content/themes/betheme-child/style.css?ver=6.4.5' type='text/css' media='all' /> <style id='mfn-custom-inline-css' type='text/css'> @supports (-ms-ime-align: auto) { } span {font-family:"Open Sans", Arial, Tahoma, sans-serif !important;} @media only screen and (min-width: 1240px) { .header-classic #Header .top_bar_left {width:1306px;} #Top_bar .menu > li > a 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text-transform:unset;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title:hover span {text-decoration:none ;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu ul {background:transparent; } .mm-vertical #Top_bar .menu > li >ul.mfn-megamenu ul a {color:#007dc5; } .mm-vertical #Top_bar .menu > li >ul.mfn-megamenu ul a:hover {background:transparent;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li a {padding:5px 0px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li a:hover {text-decoration:underline;} .mm-vertical #Top_bar .menu > li > ul {padding:20px 20px 20px 2px;} .mm-vertical #Top_bar .menu > li > ul a {padding:5px; border:0px; color:#007dc5;} .mm-vertical #Top_bar .menu > li > ul a:hover {background:transparent; } .mm-vertical #Top_bar .menu > li > ul a:hover span {text-decoration:underline ;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li {padding-top:20px; padding-bottom:25px; border-right: 0px solid rgba(0, 0, 0, 0.05);} /*for parent title in sbmenu*/ .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child {display:none; width:auto; padding-bottom:0px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child h3 {padding-top:20px; line-height:30px; border-bottom:1px solid #ccc; font-weight:600;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child h3 a{color:#444444;} /*for contact submenu*/ .mm-vertical #Top_bar .menu > li#menu-item-2437 li.mfn-megamenu-cols-2 li {} /*menu button css*/ .mm-vertical #Top_bar .menu > li#menu-item-5221 a span {background:#007dc5; color:#fff; border-radius:6px; line-height:40px; margin-top:8px; margin-left:20px;} .mm-vertical #Top_bar .menu > li#menu-item-5221 a:after {display:none;} #Top_bar.is-sticky .menu > li#menu-item-5221 > a, #Top_bar.is-sticky .menu > li#menu-item-5221 > a span {line-height:30px; margin-top:0px;} .mm-vertical #Top_bar li#menu-item-6152 a {color:#000 !important; padding-top:0px !important; white-space:nowrap; color: #007dc5 !important; font-weight: 400;} /*set width of menu item*/ .mm-vertical #Top_bar li#menu-item-2341 {width:343px; padding-left: 80px;} .mm-vertical #Top_bar li#menu-item-2348 {width:278px;} .mm-vertical #Top_bar li#menu-item-2355 {width:288px;} .mm-vertical #Top_bar li#menu-item-2363 {width:240px;} .mm-vertical #Top_bar li#menu-item-2369 {width:541px; padding-left:70px;} .mm-vertical #Top_bar li#menu-item-2379 {width:340px;} .mm-vertical #Top_bar li#menu-item-2384 {width:280px;} .mm-vertical #Top_bar li#menu-item-2391 {width:350px; padding-left:80px;} .mm-vertical #Top_bar li#menu-item-2395 {width:310px;} .mm-vertical #Top_bar li#menu-item-2403 {width:310px;} .mm-vertical #Top_bar li#menu-item-2408 {width:210px;} .mm-vertical #Top_bar li#menu-item-5918 {width:487px; padding-left:115px;} .mm-vertical #Top_bar li#menu-item-5919 {width:387px;} .mm-vertical #Top_bar li#menu-item-5920 {width:280px;} .mm-vertical #Top_bar li#menu-item-2427 {width:700px; padding-left:300px;} .mm-vertical #Top_bar li#menu-item-5235 {width:397px;} .mm-vertical #Top_bar li#menu-item-5765 {width:280px;} .mm-vertical #Top_bar li#menu-item-5235 {width:700px; padding-left:300px;} .mm-vertical #Top_bar li#menu-item-5765 {width:397px;} .mm-vertical #Top_bar li#menu-item-15082 {width:700px; padding-left:300px;} .mm-vertical #Top_bar li#menu-item-15044 {width:397px;} } @media only screen and (max-width: 1239px) { #menu > ul > li > a, a.action_button, #overlay-menu ul li a { font-size: 15px; font-weight: 400; letter-spacing: 1px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child {display:none; width:auto; padding-bottom:0px;} .mm-vertical #Top_bar #menu ul li ul li a {font-size:16px;} .mm-vertical #Top_bar #menu ul li ul li ul li a {font-size:15px;} } /* Header */ .header-classic #Top_bar { border-bottom: 0px solid #e1e1e1;} .header-classic:not(.home) #Content { border-top: 0px solid #e1e1e1; } .header-classic.home .mfn-rev-slider { border-top: 1px solid #e1e1e1; } /*Action Bar*/ #Action_bar a {color:#fff;} /* Widget */ .widget-area:before { display: none; } .aside_left .widget-area { border: 0; } /* Icon box */ .icon_box.has_border { background: #fff; border: 1px solid #e1e1e1; padding: 40px 10%; } .icon_box.no_border {background: #fff; border:0px; padding: 40px 10%;} .icon_box.has_border:after {border:0px} /* Offer box */ .offer_box { background-color: #252525; padding: 20px 20px 5px; text-align: center; } .offer_box .offer_box_list { border-width: 1px 1px 0 1px; border-style: solid; border-color: #929292; font-weight: 700; font-size: 16px; letter-spacing: 2px; margin-bottom: 20px; } .offer_box .offer_box_list a { display: block; color: #fff; border-bottom: 1px solid #929292; padding: 18px 15px; word-wrap: break-word; } /* Footer */ /*#Footer { border-top: 1px solid #e1e1e1; }*/ #Footer .widget > h4 { font-size: 18px; font-weight: 300; margin-bottom: 20px; } #Footer .footer_copy { background: #465158; border-top: 0; } .before-footer {background: #f9f9f9;} #Footer .footer_copy .social { display: none; } /**/ .top-widgets-container {float:right} .top-widgets-container .widget {padding-bottom:0px; margin-bottom:0px; float:right;margin-top:0px;} .top-widgets-container .widget ul.menu{list-style:none; padding:0px;} .top-widgets-container .widget ul.menu li {float:left; padding:12px 0px;} .top-widgets-container .widget ul.menu li a{padding:0px 20px; border-right:1px solid #ccc; line-height:15px;} .top-widgets-container .widget ul.menu li:last-child a{border-right:0px;} .top-widgets-container .widget_search .icon-search-fine {font-size:24px; line-height:40px;} .top-widgets-container .widget_search input[type="text"] {width:82%; float:right; padding:5px 10px 5px 5px; border:0px; margin-top:7px; height:30px;min-height:30px;} #menu-top-navigation .cart a {padding:0px 20px; } #menu-top-navigation .cart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; padding-right:10px; font-size:26px; color:#fff; text-indent:0;} .mobilecart {display:none; width:30px; float:right; margin-top:11px;} .mobilecart a { padding:0px; border:0px;} .mobilecart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; left:15px; font-size:26px; color:#fff; text-indent:0;} @media only screen and (min-width:320px) and (max-width:767px) { .top-widgets-container .widget {float:none;} .top-widgets-container .widget.widget_nav_menu {max-width:245px; margin:0px auto; clear:both;} .top-widgets-container .widget ul.menu li a {padding:0px 15px;} #menu-top-navigation .cart.menu-item-2449 {display:none;} #menu-top-navigation .cart a {padding:0px 10px;} .widget_archive ul li:after, .widget_nav_menu ul li:after {display:none;} .widget:after {display:none;} .top-widgets-container {float:none; margin:0px auto;} .top-widgets-container .widget_search input[type="text"] {width:80%;} .mobilecart {display:block;} .top-widgets-container .widget_search {width:254px; overflow:hidden;padding-bottom:20px; margin:0px auto;} .top-widgets-container .widget_search .searchform{width:210px; float:left;} } .icon-100 .image_wrapper img {width:150px; height:150px;} .down-heading, .heading {padding-left:17px;} @media only screen and (min-width:320px) and (max-width:767px) { .down-heading, .heading {padding:0px; text-align:center;} } .ui-tabs .ui-tabs-nav li {border-width:0px;} .page-id-2602 .ui-tabs .ui-tabs-nav li a {padding:14px 28px !important;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li {border:0px; padding:5px 0px;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li a {background-image:none; font-size:100%; color:#2991d6; padding:5px 10px 5px 20px !important;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li a:hover {text-decoration:underline;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a {color:#000; border-left:3px solid #2991d6; border-radius:0px !important; padding:5px 10px 5px 17px !important;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav {width:23%; border:0px !important;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-panel {width:76.7%; border:0px !important; padding-top:5px;} @media only screen and (min-width:768px) and (max-width:1239px) { .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li a {font-size:100%;} } @media only screen and (min-width:320px) and (max-width:767px) { .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav {width:100%;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-panel {width:100%; padding-top:15px;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li a {-webkit-box-shadow:none;-moz-box-shadow:none;box-shadow:none;} } .ui-tabs .ui-tabs-nav li h3 {margin:0px; padding:0px;} .ui-tabs .ui-tabs-nav li h3 a { text-decoration: none; display: block; padding: 14px 30px !important; font-size: 100%; font-weight:initial; } .ui-tabs .ui-tabs-panel .icon_box {padding-bottom:2px;} .ui-tabs .ui-tabs-panel .column.one-fourth {width:25%;} .ui-tabs .ui-tabs-panel .column.one-second, .ui-tabs .ui-tabs-panel .column.one-fourth, .ui-tabs .ui-tabs-panel .column.one-third, .ui-tabs .ui-tabs-panel .column.two-third{margin-left:0px; margin-right:0px;} @media only screen and (min-width: 960px) and (max-width: 1239px) { .ui-tabs .ui-tabs-nav li h3 a {font-size:100%; padding:10px 20px !important;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .ui-tabs .ui-tabs-nav li h3 a {font-size:100%; padding:5px 10px !important;} } /* Make Nav Subheadings unclickable */ .unclickable > a:hover { cursor: default;} .wp-caption-text { background: transparent; color: #075c97; font-size:90%;} .ls-wrapper h3 a {color: #075c97;} .column_sidebar_widget {float:none;} /*app notes*/ ul.clients.appnotes .client_wrapper {text-align:left; height:auto !important; line-height:auto !important; overflow:hidden;} ul.clients.appnotes li:nth-child(2n+1) {clear:both;} ul.clients.appnotes .client_wrapper img { position:relative; left:auto !important; top: auto !important; float:left; -webkit-transform: none!important; -moz-transform: none!important; transform: none!important; padding-right:20px; width:auto!important; } ul.clients.appnotes .client_wrapper h3 { line-height:20px; font-size:16px; letter-spacing:1px; padding-top:10px;} ul.clients.appnotes .client_wrapper a:hover {text-decoration:none;} @media only screen and (max-width: 640px) { ul.clients.appnotes li {width:100% !important; clear:both;} } .pager {padding-top:20px;} .pager .page-numbers {border:1px solid #ccc; padding:3px 5px;} .pager .prev.page-numbers, .pager .next.page-numbers {border:0px;} .alignleft {margin:3px 10px 10px 0px;} dl {clear:both;} .team.team_vertical img.scale-with-grid {width:100%;} .column_placeholder {margin-bottom:0px;} .icon-height img{max-height:50px;} .single .post-header {margin-left:0px; margin-right:0px;} .single .section-post-header .single-photo-wrapper.image {display:none;} .widget_mfn_menu ul li.menu-item-has-children > a::after {content: '\e868'; font-family: "mfn-icons"; right:2px; position:absolute; width:20px; height:15px; color:#ccc; } table th ul, table td ul {list-style-position:inside !important;} .sticky-white #Top_bar.is-sticky, .sticky-white #Top_bar.is-sticky #menu, #Top_bar.is-sticky {background:#fff; opacity:1;} /*Webinars latest posts*/ .parent-pageid-3663 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .parent-pageid-3663 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .parent-pageid-3663 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .parent-pageid-3663 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .parent-pageid-3663 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .parent-pageid-3663 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .parent-pageid-3663 .Latest_news ul li.category-webinars .desc h5 a{color:#007dc5;} .parent-pageid-3663 .Latest_news ul li .desc_footer .date {display:none;} .parent-pageid-3663 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .parent-pageid-3663 .Latest_news ul li .desc_footer .post-comments {display:none;} .parent-pageid-3663 .Latest_news ul li .desc_footer .button-love {display:none;} .parent-pageid-3663 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .parent-pageid-3663 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Upcoming Live Webinars*/ .page-id-2653 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .page-id-2653 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .page-id-2653 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .page-id-2653 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .page-id-2653 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .page-id-2653 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .page-id-2653 .Latest_news ul li.category-webinars .desc h5 a{color:#007dc5;} .page-id-2653 .Latest_news ul li .desc_footer .date {display:none;} .page-id-2653 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .page-id-2653 .Latest_news ul li .desc_footer .post-comments {display:none;} .page-id-2653 .Latest_news ul li .desc_footer .button-love {display:none;} .page-id-2653 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .page-id-2653 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Newsletter Archive*/ .page-id-3661 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .page-id-3661 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .page-id-3661 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .page-id-3661 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .page-id-3661 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .page-id-3661 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .page-id-3661 .Latest_news ul li.category-newsletter-archive .desc h5 a{color:#007dc5;} .page-id-3661 .Latest_news ul li .desc_footer .date {display:none;} .page-id-3661 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .page-id-3661 .Latest_news ul li .desc_footer .post-comments {display:none;} .page-id-3661 .Latest_news ul li .desc_footer .button-love {display:none;} .page-id-3661 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .page-id-3661 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Ask the Expert*/ .page-id-5348 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .page-id-5348 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .page-id-5348 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .page-id-5348 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .page-id-5348 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .page-id-5348 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .page-id-5348 .Latest_news ul li .desc h5{margin-bottom:0px;} .page-id-5348 .Latest_news ul li.category-ask-the-expert .desc h5 a{color:#007dc5;} .page-id-5348 .Latest_news ul li .desc_footer {display:none;} .page-id-5348 .Latest_news ul li .desc_footer .date {display:none;} .page-id-5348 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .page-id-5348 .Latest_news ul li .desc_footer .post-comments {display:none;} .page-id-5348 .Latest_news ul li .desc_footer .button-love {display:none;} .page-id-5348 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .page-id-5348 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; font-weight:normal; line-height:20px;} /*accordion title as h3*/ .accordion .question .title h3 {margin-bottom:0px; } .accordion .question.active .title h3{color:#2991d6} /*column padding*/ .column, .columns {margin:0px 10px 40px;} .accordion .answer .one-second.column {width:47%;} .wrap .one-second.column {width:47%;} /*.column_column ul li {margin-bottom:2px;}*/ .single .post-header {margin-bottom:10px;} /* Appnote seach box*/ .widget_appnote_search_widget {clear:both; display:block; overflow:hidden;} .widget_appnote_search_widget .widget-title {display:inline-block; float:left; margin-right:15px; margin-top:5px;} .widget_appnote_search_widget .searchform {display:inline-block; float:left;} .widget_appnote_search_widget .searchform input[type=text] {float:left; margin:0px; min-width:350px; padding:7px 10px;} .widget_appnote_search_widget .searchform input[type=submit] {background:#0065a4; margin:0px; margin-left:15px; padding:8px 20px;} @media only screen and (min-width: 320px) and (max-width: 650px) { .widget_appnote_search_widget .searchform input[type=text] {min-width:240px;} } @media only screen and (min-width: 320px) and (max-width: 519px) { .widget_appnote_search_widget .searchform input[type=submit] {margin:0px; margin-top:15px;} } /*flat box*/ .flat_box .photo_wrapper .icon {display:none;} .flat_box .photo_wrapper img.photo {transform: translateX(0px)} .flat_box .desc_wrapper {margin-left:0px;} /*center images at user profile*/ .page-id-2504 .image_frame .image_wrapper {text-align:center} .page-id-2504.if-overlay .image_frame:hover .image_wrapper img, .page-id-2504.if-overlay .image_frame.hover .image_wrapper img {transform: scale(1);} .if-overlay .image_frame .image_wrapper .image_links {display:none;} /* Changes for ClickDimensions submit button */ a.button_theme, a.tp-button.button_theme, button, input[type="submit"], input[type="reset"], input[type="button"] { background-color: #0065a4 !important; color: #ffffff !important; font-family: inherit !important; /*padding:0px;*/ } /*isotope wrapper*/ .blog_wrapper.isotope_wrapper .post-item .post-footer {background:none;} .blog_wrapper.isotope_wrapper .post-item .post-footer .post-links{border:0px;} .blog_wrapper.isotope_wrapper .grid .post-photo-wrapper {border:0px;} /* product page*/ .page-id-2582 .ui-tabs-panel .team.team_vertical .image_frame {border:0px;} .page-id-2582 .ui-tabs-panel .team.team_vertical hr.hr_color {background:none;} .page-id-2582.if-overlay .ui-tabs-panel .team.team_vertical .image_frame:hover .image_wrapper img, .page-id-2582.if-overlay .ui-tabs-panel .team.team_vertical .image_frame.hover .image_wrapper img {-webkit-transform: scale(1); -moz-transform: scale(1); -o-transform: scale(1); transform: scale(1); } .page-id-2582 .ui-tabs-panel .one-third.column:nth-child(4n-3) {margin-left:10px; margin-right:17px; } .page-id-2582 .ui-tabs-panel .one-third.column:nth-child(4n-2) {margin-left:18px; margin-right:17px; } .page-id-2582 .ui-tabs-panel .one-third.column:nth-child(4n-1) {margin-left:18px; margin-right:10px; } .page-id-2582 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:65px; margin-right:52px; } .page-id-2582 .ui-tabs-panel .one-second.column:nth-child(even) {margin-left:53px; margin-right:65px; } @media only screen and (min-width: 1301px) { .page-id-2582 .ui-tabs-panel .one-third.column {width:30.9%;} .page-id-2582 .ui-tabs-panel .one-second.column {width:40.2%;} } @media only screen and (min-width: 1240px) and (max-width: 1300px) { .page-id-2582 .ui-tabs-panel .one-third.column {width:30.7%;} .page-id-2582 .ui-tabs-panel .one-second.column {width:47.6%;} } @media only screen and (min-width: 960px) and (max-width: 1239px) { .page-id-2582 .ui-tabs-panel .one-third.column {width:29.9%;} .page-id-2582 .ui-tabs-panel .one-second.column {width:36.6%;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .page-id-2582 .ui-tabs-panel .one-third.column {width:28.7%;} .page-id-2582 .ui-tabs-panel .one-second.column {width:38.9%;} } @media only screen and (min-width: 320px) and (max-width: 767px) { .page-id-2582 .ui-tabs-panel .one-third.column {margin-left:0px; margin-right:0px;} .page-id-2582 .ui-tabs-panel .one-second.column:nth-child(even), .page-id-2582 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:0px; margin-right:0px;} } .uk-margin-small-right {vertical-align:middle; margin-top:-2px;} .page-id-2647 .ui-tabs .ui-tabs-panel .column.one-second {margin-bottom:0px;} .page-id-3641 .blog_wrapper .classic .post-photo-wrapper {border-color:transparent;} @media only screen and (min-width: 960px) { .page-id-3641 .blog_wrapper .classic .post-photo-wrapper {width:12%;} .page-id-3641 .blog_wrapper .classic .post-desc-wrapper {width:88%;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .page-id-3641 .blog_wrapper .classic .post-photo-wrapper { width:25%} .page-id-3641 .blog_wrapper .classic .post-desc-wrapper {width:75%;} } @media only screen and (min-width: 768px) { .page-id-2640 .column.column_pricing_item {margin:0px 1% 40px;} } /* contact form 7 submit button*/ .wpcf7-form-control.wpcf7-submit {padding:10px 30px;} .wpcf7 .red {color:#990000;} .wpcf7 span.wpcf7-list-item {display:block; margin:0px;} .wpcf7 label {font-weight:400;} #lostusername {border:1px solid #ddd; padding:30px 20px 0px;} #lostusername .input {width:100%;} #lostusername .button {padding:10px 20px;} .ur-frontend-form button[type="submit"], .ur-frontend-form input[type="submit"] {float:none;} .page-id-2690.if-overlay .image_frame:hover .image_wrapper img, .page-id-2690.if-overlay .image_frame.hover .image_wrapper img {transform: scale(1);} .page-id-2690 .team .desc_wrapper hr {display:none;} .page-id-2690 .ui-tabs .ui-tabs-panel .column.one-second, .page-id-2690 .ui-tabs .ui-tabs-panel .column.one-fourth, .page-id-2690 .ui-tabs .ui-tabs-panel .column.one-third, .page-id-2690 .ui-tabs .ui-tabs-panel .column.two-third {margin-left:10px; margin-right:10px;} .page-id-2690 .ui-tabs .ui-tabs-panel .column.one-fourth {width:23.4%;} /* webinars seach box*/ .widget_webinars_search_widget {clear:both; display:block; overflow:hidden;} .widget_webinars_search_widget .widget-title {display:inline-block; float:left; margin-right:15px; margin-top:5px;} .widget_webinars_search_widget .searchform {display:inline-block; float:left;} .widget_webinars_search_widget .searchform input[type=text] {float:left; margin:0px; min-width:350px; padding:7px 10px;} .widget_webinars_search_widget .searchform input[type=submit] {background:#0065a4; margin:0px; margin-left:15px; padding:8px 20px;} @media only screen and (min-width: 320px) and (max-width: 650px) { .widget_webinars_search_widget .searchform input[type=text] {min-width:240px;} } @media only screen and (min-width: 320px) and (max-width: 519px) { .widget_webinars_search_widget .searchform input[type=submit] {margin:0px; margin-top:15px;} } /*Recent Webinars nav issue fix*/ .page-id-3665 li#menu-item-2421.current-menu-ancestor:not(.hover) a {color:#000000;} .page-id-3665 li#menu-item-2421.current-menu-ancestor:not(.hover) a:after {display:none;} /*login-in nav issue fix*/ .page-id-5451 li#menu-item-2390.current-menu-ancestor:not(.hover) a {color:#000000;} .page-id-5451 li#menu-item-2390.current-menu-ancestor:not(.hover) a:after {display:none;} /* Contact Customer Support nav issue fix*/ .page-id-9343 li#menu-item-2390.current-menu-ancestor:not(.hover) a {color:#000000;} .page-id-9343 li#menu-item-2390.current-menu-ancestor:not(.hover) a:after {display:none;} /* custom seach box*/ .search #Content .searchform {display:inline-block; float:left;} .search #Content .searchform h4{clear:both; padding:10px 0px; margin:0px;} .search #Content .searchform input[type=text] {float:left; margin:0px; min-width:350px; padding:7px 10px;} .search #Content .searchform select {-webkit-appearance:menulist; padding:7px 10px;} .search #Content .searchform input[type=submit] {background:#0065a4; margin:0px; margin-left:15px; padding:8px 20px;} @media only screen and (min-width: 320px) and (max-width: 650px) { .search #Content .searchform input[type=text] {min-width:240px;} } @media only screen and (min-width: 320px) and (max-width: 519px) { .search #Content .searchform input[type=submit] {margin:0px; margin-top:15px;} } .wpcf7-form .anr_captcha_field {position:absolute;} /*newsletter archive tables remove border*/ .post.category-newsletter-archive .post-wrapper-content table td {box-shadow:none; border:0px;} .be-ix-link-block {max-width:750px; text-align:center; margin:0px auto; padding-top:20px;} #Footer .widgets_wrapper .column {margin:0px 9px 0px;} @media only screen and (min-width: 768px) and (max-width: 959px) { #Footer .widgets_wrapper .column {margin:0px 5px 0px;} } /*Login page popup */ .page-id-5451.logged-in #pum-8728 {display:none !important;} #cookie-law-info-bar a {color:#fff; text-decoration:underline;} #cookie-law-info-bar a:hover {text-decoration:none;} #cookie-law-info-bar .cli-plugin-button {text-decoration:none;} @media only screen and (min-width: 320px) and (max-width: 767px) { #cookie-law-info-again{display:none !important;} } /*After Multi Language SETUP*/ #Action_bar .column {overflow:visible;} .top-widgets-container .widget_search input[type="text"] {margin-top:5px;} .widget.widget_nav_menu::after, .widget.widget_icl_lang_sel_widget::after, .widget_archive ul li:after, .widget_nav_menu ul li:after {display:none;} .wpml-ls-legacy-dropdown-click {margin-top:6px; } .wpml-ls-legacy-dropdown-click a, .wpml-ls-legacy-dropdown-click a:hover, .wpml-ls-legacy-dropdown-click a:focus, .wpml-ls-legacy-dropdown-click .wpml-ls-current-language:hover>a {color:#626262 !important; border:0px;text-decoration:none;} .wpml-ls-legacy-dropdown-click .wpml-ls-sub-menu {z-index:999;} .top-widgets-container h2.title {display:none;} @media only screen and (min-width: 320px) and (max-width: 767px) { .wpml-ls-legacy-dropdown-click {margin:6px auto 0px; } .top-widgets-container .widget_search {clear:both;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .wpml-ls-legacy-dropdown-click {width:auto;} } #menu-top-navigation-ch .cart a {padding:0px 20px; } #menu-top-navigation-ch .cart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; padding-right:10px; font-size:26px; color:#fff; text-indent:0;} @media only screen and (min-width:320px) and (max-width:767px) { #menu-top-navigation-ch .cart.menu-item-2449 {display:none;} #menu-top-navigation-ch .cart a {padding:0px 10px;} } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-13087 a span {background:#007dc5; color:#fff; border-radius:10px; line-height:40px; margin-top:8px; margin-left:20px;} .mm-vertical #Top_bar .menu > li#menu-item-13087 a:after {display:none;} #Top_bar.is-sticky .menu > li#menu-item-13087 > a, #Top_bar.is-sticky .menu > li#menu-item-13087 > a span {line-height:30px; margin-top:0px;} } .post-9911 .wpcf7 span.wpcf7-list-item{width: 260px;display: inline-block;} .post-9911 span.wpcf7-form-control-wrap{margin-bottom:20px;} .post-9911 .wpcf7-list-item-label{color:#000000;} .post-9911 .unique .one-third.column{width:30.333% !important;} .post-9911 .unique .required{color: #ff0000; font-size: 13px; padding: 0 5px 0 5px;} .post-9911 .wrap .one-second.column{width:32%;} .post-9911 .wpcf7 hr.small {color:#000000;background-color:#000000;height:1px; max-width: 440px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .post-9911 .wpcf7 hr.large{color:#000000;background-color:#000000;height:1px; max-width: 760px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .post-9911 .wpcf7 p strong{font-weight:normal; font-size:18px; color:#1F497D;} @media only screen and (min-width: 320px) and (max-width: 959px) { .post-9911 .wpcf7 span.wpcf7-list-item{width: 100%;} .post-9911 .unique .one-third.column{width:100% !important;} .post-9911 .wrap .one-second.column{width:100%;} .post-9911 span.wpcf7-form-control-wrap {margin-bottom:0px;} } /*remove meta from search-results*/ .search.search-results .post-item .post-meta {display:none;} .search.search-results .post-item .post-desc {padding-left:0px;} /*footer update*/ /*#Footer .widgets_wrapper {padding-bottom:10px;} #Footer .widgets_wrapper .column {margin-bottom:10px;} #Footer #custom_html-4 > h4 {text-align:center;} #Footer #custom_html-14 > h4 {text-align:center;} #Footer #custom_html-9 {max-width:160px; margin:0px auto; clear:both; padding-top:25px;} .footer-resources {max-width:80%; margin:0px auto; } .footer-resources li {float:left; line-height:28px;} .footer-resources li:nth-child(1), .footer-resources li:nth-child(6){width:12%; clear:both;} .footer-resources li:nth-child(2), .footer-resources li:nth-child(7){width:28%;} html[lang="zh-hans"] .footer-resources li:nth-child(1), html[lang="zh-hans"] .footer-resources li:nth-child(6){width:15%; clear:both;} html[lang="zh-hans"] .footer-resources li:nth-child(2), html[lang="zh-hans"] .footer-resources li:nth-child(7){width:25%;} .footer-resources li:nth-child(3), .footer-resources li:nth-child(8){width:17%;} .footer-resources li:nth-child(4), .footer-resources li:nth-child(9){width:18%;} .footer-resources li:nth-child(5), .footer-resources li:nth-child(10){width:25%;} @media only screen and (min-width:320px) and (max-width:1239px) { .footer-resources {max-width:60%; } .footer-resources li:nth-child(1), .footer-resources li:nth-child(6){width:50%; clear:none;} .footer-resources li:nth-child(2), .footer-resources li:nth-child(7){width:50%;} .footer-resources li:nth-child(3), .footer-resources li:nth-child(8){width:50%;} .footer-resources li:nth-child(4), .footer-resources li:nth-child(9){width:50%;} .footer-resources li:nth-child(5), .footer-resources li:nth-child(10){width:50%;} } @media only screen and (min-width:320px) and (max-width:767px) { .footer-resources {max-width:100%; } #Footer #custom_html-4 > h4 {text-align:left;} #Footer #custom_html-14 > h4 {text-align:left;} #Footer #custom_html-9 {max-width:100%;} } */ .tabs_vertical.ui-tabs .ui-tabs-nav li a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li a.button:hover, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button:hover { background: #007dc5; color: #fff; border-radius: 10px !important; text-align: center; margin-left: 20px; margin-top: 20px; display:inline-block; text-decoration: none; padding: 0px 20px !important; border:0px; text-transform: uppercase; line-height:30px; font-size: 15px; font-weight: 400;letter-spacing: 1px;} @media only screen and (min-width:320px) and (max-width:767px) { .tabs_vertical.ui-tabs .ui-tabs-nav li h3, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active h3 {text-align:center;} .tabs_vertical.ui-tabs .ui-tabs-nav li a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li a.button:hover, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button:hover {margin:20px auto;} } /*Webinars latest posts zh-hans*/ .parent-pageid-11725 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .parent-pageid-11725 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .parent-pageid-11725 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .parent-pageid-11725 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .parent-pageid-11725 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .parent-pageid-11725 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .parent-pageid-11725 .Latest_news ul li.category-webinars-zh-hans .desc h5 a{color:#007dc5;} .parent-pageid-11725 .Latest_news ul li .desc_footer .date {display:none;} .parent-pageid-11725 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .parent-pageid-11725 .Latest_news ul li .desc_footer .post-comments {display:none;} .parent-pageid-11725 .Latest_news ul li .desc_footer .button-love {display:none;} .parent-pageid-11725 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .parent-pageid-11725 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Upcoming Live Webinars zh-hans*/ .page-id-11764 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .page-id-11764 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .page-id-11764 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .page-id-11764 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .page-id-11764 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .page-id-11764 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .page-id-11764 .Latest_news ul li.category-webinars-zh-hans .desc h5 a{color:#007dc5;} .page-id-11764 .Latest_news ul li .desc_footer .date {display:none;} .page-id-11764 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .page-id-11764 .Latest_news ul li .desc_footer .post-comments {display:none;} .page-id-11764 .Latest_news ul li .desc_footer .button-love {display:none;} .page-id-11764 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .page-id-11764 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Request Product Quote zh-hans*/ .page-id-12227 .wpcf7 span.wpcf7-list-item{width: 260px;display: inline-block;} .page-id-12227 span.wpcf7-form-control-wrap{margin-bottom:20px;} .page-id-12227 .wpcf7-list-item-label{color:#000000;} .page-id-12227 .unique .one-third.column{width:29% !important;} .page-id-12227 .unique .required{color: #ff0000; font-size: 13px; padding: 0 5px 0 5px;} .page-id-12227 .wrap .one-second.column{width:32%;} .page-id-12227 .wpcf7 hr.small {color:#000000;background-color:#000000;height:1px; max-width: 440px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .page-id-12227 .wpcf7 hr.large{color:#000000;background-color:#000000;height:1px; max-width: 760px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .page-id-12227 .wpcf7 p strong{font-weight:normal; font-size:18px; color:#1F497D;} @media only screen and (min-width: 320px) and (max-width: 959px) { .page-id-12227 .wpcf7 span.wpcf7-list-item{width: 100%;} .page-id-12227 .unique .one-third.column{width:100% !important;} .page-id-12227 .wrap .one-second.column{width:100%;} .page-id-12227 span.wpcf7-form-control-wrap {margin-bottom:0px;} } /*Request Product Info zh-hans*/ .page-id-12612 .wpcf7 span.wpcf7-list-item{width: 260px;display: inline-block;} .page-id-12612 span.wpcf7-form-control-wrap{margin-bottom:20px;} .page-id-12612 .wpcf7-list-item-label{color:#000000;} .page-id-12612 .unique .one-third.column{width:29% !important;} .page-id-12612 .unique .required{color: #ff0000; font-size: 13px; padding: 0 5px 0 5px;} .page-id-12612 .wrap .one-second.column{width:32%;} .page-id-12612 .wpcf7 hr.small {color:#000000;background-color:#000000;height:1px; max-width: 440px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .page-id-12612 .wpcf7 hr.large{color:#000000;background-color:#000000;height:1px; max-width: 760px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .page-id-12612 .wpcf7 p strong{font-weight:normal; font-size:18px; color:#1F497D;} @media only screen and (min-width: 320px) and (max-width: 959px) { .page-id-12612 .wpcf7 span.wpcf7-list-item{width: 100%;} .page-id-12612 .unique .one-third.column{width:100% !important;} .page-id-12612 .wrap .one-second.column{width:100%;} .page-id-12612 span.wpcf7-form-control-wrap {margin-bottom:0px;} } /*Team in zh-hans*/ .page-id-12002.if-overlay .image_frame:hover .image_wrapper img, .page-id-12002.if-overlay .image_frame.hover .image_wrapper img {transform: scale(1);} .page-id-12002 .team .desc_wrapper hr {display:none;} .page-id-12002 .ui-tabs .ui-tabs-panel .column.one-second, .page-id-12002 .ui-tabs .ui-tabs-panel .column.one-fourth, .page-id-12002 .ui-tabs .ui-tabs-panel .column.one-third, .page-id-12002 .ui-tabs .ui-tabs-panel .column.two-third {margin-left:10px; margin-right:10px;} .page-id-12002 .ui-tabs .ui-tabs-panel .column.one-fourth {width:23.4%;} /* product page in zh-hans*/ .page-id-12156 .ui-tabs-panel .team.team_vertical .image_frame {border:0px;} .page-id-12156 .ui-tabs-panel .team.team_vertical hr.hr_color {background:none;} .page-id-12156.if-overlay .ui-tabs-panel .team.team_vertical .image_frame:hover .image_wrapper img, .page-id-12156.if-overlay .ui-tabs-panel .team.team_vertical .image_frame.hover .image_wrapper img {-webkit-transform: scale(1); -moz-transform: scale(1); -o-transform: scale(1); transform: scale(1); } .page-id-12156 .ui-tabs-panel .one-third.column:nth-child(4n-3) {margin-left:10px; margin-right:17px; } .page-id-12156 .ui-tabs-panel .one-third.column:nth-child(4n-2) {margin-left:18px; margin-right:17px; } .page-id-12156 .ui-tabs-panel .one-third.column:nth-child(4n-1) {margin-left:18px; margin-right:10px; } .page-id-12156 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:65px; margin-right:52px; } .page-id-12156 .ui-tabs-panel .one-second.column:nth-child(even) {margin-left:53px; margin-right:65px; } @media only screen and (min-width: 1301px) { .page-id-12156 .ui-tabs-panel .one-third.column {width:30.9%;} .page-id-12156 .ui-tabs-panel .one-second.column {width:40.2%;} } @media only screen and (min-width: 1240px) and (max-width: 1300px) { .page-id-12156 .ui-tabs-panel .one-third.column {width:30.7%;} .page-id-12156 .ui-tabs-panel .one-second.column {width:47.6%;} } @media only screen and (min-width: 960px) and (max-width: 1239px) { .page-id-12156 .ui-tabs-panel .one-third.column {width:29.9%;} .page-id-12156 .ui-tabs-panel .one-second.column {width:36.6%;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .page-id-12156 .ui-tabs-panel .one-third.column {width:28.7%;} .page-id-12156 .ui-tabs-panel .one-second.column {width:38.9%;} } @media only screen and (min-width: 320px) and (max-width: 767px) { .page-id-12156 .ui-tabs-panel .one-third.column {margin-left:0px; margin-right:0px;} .page-id-12156 .ui-tabs-panel .one-second.column:nth-child(even), .page-id-12156 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:0px; margin-right:0px;} } /*Recent Webinars nav issue fix for zh-hans*/ .page-id-11728 li#menu-item-13052.current-menu-ancestor:not(.hover) a {color:#000000;} .page-id-11728 li#menu-item-13052.current-menu-ancestor:not(.hover) a:after {display:none;} .large h3{font-size:22px; line-height:30px;} .small {margin-left:25px;} sup {display:inline-block; margin-top:-4px;} sub {display:inline-block; margin-bottom:-4px;} sup, sub {display:inline-table;} .ui-tabs-panel .one-second.column {width:50%;} .parent-pageid-2582 .ui-tabs-panel td {text-align:left !important;} .ls-borderlessdark3d .ls-shadow { display: none !important;} #menu-top-navigation-de .cart a {padding:0px 20px;} #menu-top-navigation-de .cart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; padding-right:15px; font-size:26px; color:#fff; text-indent:0;} @media only screen and (min-width:320px) and (max-width:767px) { #menu-top-navigation-de .cart.menu-item-14543{display:none;} #menu-top-navigation-de .cart a {padding:0px 10px;} } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-15087 a span {background:#007dc5; color:#fff; border-radius:10px; line-height:40px; margin-top:8px; margin-left:20px;} .mm-vertical #Top_bar .menu > li#menu-item-15087 a:after {display:none;} #Top_bar.is-sticky .menu > li#menu-item-15087 > a, #Top_bar.is-sticky .menu > li#menu-item-15087 > a span {line-height:30px; margin-top:0px;} } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar li#menu-item-12984 a {color:#000 !important; padding-top:20px !important;} .mm-vertical #Top_bar li#menu-item-14978 a {color:#000 !important; padding-top:20px !important;} } #Footer #custom_html-15 > h4 {text-align:center;} /* product page German */ .page-id-14845 .ui-tabs-panel .team.team_vertical .image_frame {border:0px;} .page-id-14845 .ui-tabs-panel .team.team_vertical hr.hr_color {background:none;} .page-id-14845.if-overlay .ui-tabs-panel .team.team_vertical .image_frame:hover .image_wrapper img, .page-id-14295.if-overlay .ui-tabs-panel .team.team_vertical .image_frame.hover .image_wrapper img {-webkit-transform: scale(1); -moz-transform: scale(1); -o-transform: scale(1); transform: scale(1); } .page-id-14845 .ui-tabs-panel .one-third.column:nth-child(4n-3) {margin-left:10px; margin-right:17px; } .page-id-14845 .ui-tabs-panel .one-third.column:nth-child(4n-2) {margin-left:18px; margin-right:17px; } .page-id-14845 .ui-tabs-panel .one-third.column:nth-child(4n-1) {margin-left:18px; margin-right:10px; } .page-id-14845 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:65px; margin-right:52px; } .page-id-14845 .ui-tabs-panel .one-second.column:nth-child(even) {margin-left:53px; margin-right:65px; } @media only screen and (min-width: 1301px) { .page-id-14845 .ui-tabs-panel .one-third.column {width:30.9%;} .page-id-14845 .ui-tabs-panel .one-second.column {width:40.2%;} } @media only screen and (min-width: 1240px) and (max-width: 1300px) { .page-id-14845 .ui-tabs-panel .one-third.column {width:30.7%;} .page-id-14845 .ui-tabs-panel .one-second.column {width:47.6%;} } @media only screen and (min-width: 960px) and (max-width: 1239px) { .page-id-14845 .ui-tabs-panel .one-third.column {width:29.9%;} .page-id-14845 .ui-tabs-panel .one-second.column {width:36.6%;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .page-id-14845 .ui-tabs-panel .one-third.column {width:28.7%;} .page-id-14845 .ui-tabs-panel .one-second.column {width:38.9%;} } @media only screen and (min-width: 320px) and (max-width: 767px) { .page-id-14845 .ui-tabs-panel .one-third.column {margin-left:0px; margin-right:0px;} .page-id-14845 .ui-tabs-panel .one-second.column:nth-child(even), .page-id-14845 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:0px; margin-right:0px;} } .parent-pageid-14845 .ui-tabs-panel td {text-align:left !important;} .button_align.align_left .button {text-align:left; padding:10px 0;} .button_align.align_left .as_tab {font-size: 18px; line-height: 20px; font-weight: 400; background:#fff; letter-spacing: 0px; color: #2991d6 !important; padding: 5px 10px 5px 20px;margin:0; } .button_align.align_left .button.as_tab:hover {text-decoration:underline !important; background-color:#fff!important;} .button_align.align_left .button.as_tab:after {display:none;} .button_align.align_left .button.as_tab.active {color: #000; border-left: 3px solid #2991d6; border-radius: 0px !important; padding: 5px 10px 5px 17px !important;} .button_align.align_left .button.as_tab .button_label {margin:0!important;} .button_align.align_left .button.as_tab_btn { background: #007dc5; color: #fff; border-radius: 10px !important; text-align: center; margin-left: 20px; margin-top: 20px; display: inline-block; text-decoration: none; padding: 0px 20px !important; border: 0px; text-transform: uppercase; line-height: 30px; font-size: 15px; font-weight: 400; letter-spacing: 1px; } /*Japan language*/ #Footer #custom_html-22 > h4 { text-align: center; } #menu-top-navigation-ja .cart a {padding:0px 20px;} #menu-top-navigation-ja .cart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; padding-right:15px; font-size:26px; color:#fff; text-indent:0;} @media only screen and (min-width:320px) and (max-width:767px) { #menu-top-navigation-ja .cart.menu-item-2449 {display:none;} #menu-top-navigation-ja .cart a {padding:0px 10px;} } .mm-vertical #Top_bar .menu > li#menu-item-18403 a span {background:#007dc5; color:#fff; border-radius:10px; line-height:40px; margin-top:8px; margin-left:20px;} .mm-vertical #Top_bar .menu > li#menu-item-18403 a:after {display:none;} #Top_bar.is-sticky .menu > li#menu-item-18403 > a, #Top_bar.is-sticky .menu > li#menu-item-18403 > a span {line-height:30px; margin-top:0px;} @media only screen and (min-width: 1240px) { #Top_bar #menu-german-main-menu > li > a span:not(.description) { padding: 0 3px; font-size:14px; } } body, ul.timeline_items, .icon_box a .desc, .icon_box a:hover .desc, .feature_list ul li a, .list_item a, .list_item a:hover, .widget_recent_entries ul li a, .flat_box a, .flat_box a:hover, .story_box .desc, .content_slider.carousel ul li a .title, .content_slider.flat.description ul li .desc, .content_slider.flat.description ul li a .desc, .post-nav.minimal a i { color: #333333 !important; } .column_column ul, .column_column ol, .the_content_wrapper:not(.is-elementor) ul, .the_content_wrapper:not(.is-elementor) ol { color: #333333 !important; } .hero { margin-top: 20px; } .bg-hero { background: radial-gradient(circle at 100% 0,#ebf1f2 0% 30%,#0e81a7 100%); } .hero-full { min-height: 450px; background-color: rgba(0, 0, 0, 0.5); } .column, .columns { margin: 0px 8px 40px; } .image-container { position: relative; display: inline-block; /* oder 'block' je nach Layout */ } .ribbon { position: absolute; top: 10px; /* Abstand von oben */ left: -20px; /* Verschiebung nach links, um das Ribbon schräg zu positionieren */ background-color: #FF5733; /* Farbe des Ribbons */ color: white; /* Textfarbe */ padding: 5px 15px; /* Innenabstand */ transform: rotate(-0deg); /* Dreht das Ribbon */ transform-origin: 0 0; /* Setzt den Drehpunkt */ font-size: 14px; /* Schriftgröße */ box-shadow: 2px 2px 5px rgba(0,0,0,0.3); /* Schatten für 3D-Effekt */ z-index: 10; /* Stellt sicher, dass das Ribbon über dem Bild liegt */ } .event-badge { position: relative; margin-right: 10px; border-radius: 6px; top: 0px; /* Abstand von oben */ left: 0px; /* Verschiebung nach links, um das Ribbon schräg zu positionieren */ background-color: #007dc5; /* Farbe des Ribbons */ color: white; /* Textfarbe */ padding: 2px 8px; /* Innenabstand */ font-size: 10px; /* Schriftgröße */ box-shadow: 1px 1px 2px rgba(0,0,0,0.3); /* Schatten für 3D-Effekt */ z-index: 10; /* Stellt sicher, dass das Ribbon über dem Bild liegt */ } .btn-default { background: #007dc5; color: #fff; border-radius: 6px; line-height: 40px; margin-top: 8px; margin-left: 20px; padding: 0px 10px; } .wpcf7 form.sent .wpcf7-response-output { color: #ffffff; background-color: #46b450; } .jumpto { padding-top: 100px; /* Adjust this value to match your header's height */ margin-top: -100px; /* This should be the same value as padding-top but negative */ } .post-item { margin-bottom: 40px; position: relative; float: left; width: 100%; border: 1px solid #ccc; padding: 20px; background-color: #f8f8f8; } .post-links { background-color: #007dc5; border: 1px solid #ccc; padding: 5px 20px; } #field_1_18, #field_1_113, #field_1_20, #field_1_21, #field_1_22, #field_1_23, #field_1_24, #field_1_25, #field_1_26, #field_1_27, #field_1_49, #field_1_51, #field_1_52, #field_1_53, #field_1_54, #field_1_56, #field_1_57, #field_1_58, #field_1_59, #field_1_60, #field_1_62, #field_1_63, #field_1_64, #field_1_65, #field_1_67, #field_1_68, #field_1_70, #field_1_72, #field_1_98, #field_1_99, #field_1_100, #field_1_101, #field_1_102, #field_1_103, #field_1_104, #field_1_107, #field_1_108, #field_1_122, #field_1_123, #field_1_124, #field_1_125, #field_1_126, #field_1_127, #field_1_128, #field_1_136 { background-color: #E8E8E8; padding: 20px 30px; border: 1px solid #C4C4C4; border-bottom: 1px solid #C4C4C4; margin-top: -20px; } #field_1_73, #field_1_74, #field_1_75, #field_1_76, #field_1_77, #field_1_78, #field_1_82, #field_1_83, 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href="https://store.wyatt.com/"></a></div> </div> </div> </div> </div> </div> </header> </div>  <div id="Content" role="main"> <div class="content_wrapper clearfix"> <main class="sections_group"> <div class="entry-content" itemprop="mainContentOfPage"> <div class="mfn-builder-content mfn-default-content-buider"><section class="section mcb-section mfn-default-section mcb-section-uzchk1kf2 default-width" style="" ><div class="mcb-background-overlay"></div><div class="section_wrapper mfn-wrapper-for-wraps mcb-section-inner mcb-section-inner-uzchk1kf2"><div class="wrap mcb-wrap mcb-wrap-gnrqdekl one-fourth tablet-one-fourth laptop-one-fourth mobile-one sticky-desktop clearfix" data-desktop-col="one-fourth" data-laptop-col="laptop-one-fourth" data-tablet-col="tablet-one-fourth" data-mobile-col="mobile-one" style="--sticky-offset-desktop:110px" ><div class="mcb-wrap-inner mcb-wrap-inner-gnrqdekl mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="wrap mcb-wrap mcb-wrap-0tlr96ljm one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-0tlr96ljm mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-zx50hiuh9 one laptop-one tablet-one mobile-one column_table_of_contents mfn-item-custom-width" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-zx50hiuh9 mcb-item-table_of_contents-inner"><div class="table_of_content clearfix " data-tags="H2 H3"><div class="title"><h4 class="title-inner">Table of contents</h4></div><div class="table_of_content_wrapper"><ol class="mfn_toc_numbers"><li data-depth="1" ><i></i><a class="scroll" href="#introduction">Introduction</a></li><li class="mfn_toc_nested" data-depth="2"><ol><li><i></i><a class="scroll" href="#how-light-scattering-measures-pc">How light scattering measures PC</a></li><li><i></i><a class="scroll" href="#concentration-of-small-viral-vectors">Concentration of small viral vectors</a></li><li><i></i><a class="scroll" href="#applications">Applications</a></li></ol><li><i></i><a class="scroll" href="#methods">Methods</a></li><li class="mfn_toc_nested" data-depth="2"><ol><li><i></i><a class="scroll" href="#sec-fff-mals">SEC/FFF-MALS</a></li><li><i></i><a class="scroll" href="#mals-for-pat">MALS for PAT</a></li><li><i></i><a class="scroll" href="#dls">DLS</a></li><li><i></i><a class="scroll" href="#dls-in-qc-and-at-line-pat">DLS in QC and at-line PAT</a></li></ol><li><i></i><a class="scroll" href="#additional-resources">Additional Resources</a></li><li class="mfn_toc_nested" data-depth="2"><ol><li><i></i><a class="scroll" href="#selected-resources">Selected Resources</a></li><li><i></i><a class="scroll" href="#selected-references">Selected References</a></li></ol></div></div> </div></div></div></div></div></div><div class="wrap mcb-wrap mcb-wrap-sdjvcmiig three-fourth tablet-three-fourth laptop-three-fourth mobile-one clearfix" data-desktop-col="three-fourth" data-laptop-col="laptop-three-fourth" data-tablet-col="tablet-three-fourth" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-sdjvcmiig mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="wrap mcb-wrap mcb-wrap-fitcdnj9d one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-fitcdnj9d mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-43e713b7c three-fifth laptop-three-fifth tablet-three-fifth mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-43e713b7c mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h1>Particle Concentration</h1> <p>Light scattering measures the concentration of large ensembles of nanoparticles, overcoming the limitations of single-particle counting methods such as electron microscopy, NTA or TRPS that provide low statistical sampling yet require stringent sample preparation and dilution to a narrow concentration window.</p> <p>Furthermore, light scattering instruments are not clogged by larger-than-expected particles, and the only manual labor involved is usually just pipetting or dispensing into a vial, cuvette or microwell plate.</p> <p>The light scattering techniques Wyatt provides for concentration analysis are based on <a href="/library/theory/understanding-multi-angle-static-light-scattering.html">multi-angle light scattering (MALS)</a> and <a href="/library/theory/dynamic-light-scattering-theory.html">dynamic light scattering (DLS)</a>. They trade off resolution, speed and sample volume, in order to meet the requirements of high-throughput screening, detailed characterization or real-time operation.</p></div></div></div><div class="column mcb-column mcb-item-f5172107f two-fifth laptop-two-fifth tablet-two-fifth mobile-one column_column" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-f5172107f mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><div align="center"> <p><img fetchpriority="high" src="/wp-content/uploads/2024/01/WAT-Stack-Optilab-DAWN-Eclipse-COB-700-2.jpg" align="center"></p> <p align="left" style="color:#075c97;font-size:14px">SEC-MALS and FFF-MALS provide quantitative and accurate particle concentrations and size distributions. Measurements are fully automated with industry-standard autosamplers and pumps.</p> </div></div></div></div></div></div><div id="introduction" class="wrap mcb-wrap mcb-wrap-elp5u04e one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-elp5u04e mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-ah6iyv0ti one laptop-one tablet-one mobile-one column_heading" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-ah6iyv0ti mcb-item-heading-inner"><h2 class="title">Introduction</h2></div></div></div></div><div id="how-light-scattering-measures-pc" class="wrap mcb-wrap mcb-wrap-bh7j0iofe one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-bh7j0iofe mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-xsp41m5ag one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-xsp41m5ag mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h3>How light scattering measures PC</h3> <p>Particle concentration (PC) is calculated from scattered intensity, particle volume and the refractive indices <i>n</i><sub>particle</sub>, <i>n</i><sub>solvent</sub> of the particle material and the solvent.</p></div></div></div><div class="column mcb-column mcb-item-grvw443pu three-fifth laptop-three-fifth tablet-three-fifth mobile-one column_image" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-grvw443pu mcb-item-image-inner"><div class="image_frame image_item no_link scale-with-grid alignnone no_border" ><div class="image_wrapper "><img width="882" height="186" src="https://www.wyatt.com/wp-content/uploads/2024/11/equation.webp" class="scale-with-grid" alt="" style="" decoding="async" fetchpriority="high" srcset="https://www.wyatt.com/wp-content/uploads/2024/11/equation.webp 882w, https://www.wyatt.com/wp-content/uploads/2024/11/equation-300x63.webp 300w, https://www.wyatt.com/wp-content/uploads/2024/11/equation-768x162.webp 768w, https://www.wyatt.com/wp-content/uploads/2024/11/equation-150x32.webp 150w, https://www.wyatt.com/wp-content/uploads/2024/11/equation-480x101.webp 480w" sizes="(max-width:767px) 480px, (max-width:882px) 100vw, 882px" /></div></div> </div></div><div class="column mcb-column mcb-item-tzdv5izci one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-tzdv5izci mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""> <ul> <li>In addition to scattered intensity, both MALS and DLS determine particle size, from which the particle’s volume is calculated.</li> <li>Refractive indices are taken from the literature or calculated from the particle composition if it is not homogeneous; the particle’s effective <i>n</i><sub>particle</sub> may also be calibrated against a known concentration of identical particles.</li> </ul> <p>That’s all it takes! More details may be found in the <a href="/library/theory/understanding-multi-angle-static-light-scattering.html">MALS theory</a> and <a href="/library/theory/dynamic-light-scattering-theory.html">DLS theory pages</a>.</p></div></div></div></div></div><div id="concentration-of-small-viral-vectors" class="wrap mcb-wrap mcb-wrap-szyzdduqo one tablet-one laptop-one mobile-one wrap mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-szyzdduqo mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-b9bpc00li two-third laptop-two-third tablet-two-third mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-b9bpc00li mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h3>Concentration of small viral vectors</h3> <p>The physical titer of small viruses such as adeno-associated virus (AAV) is measured differently than that of larger viruses when using SEC-MALS or FFF-MALS, since they are found at the very lower limit of size for the usual particle concentration analysis method.</p> <p>Here, MALS, UV and dRI signals are combined to determine the molar mass of the virus and the overall eluting mass in each peak. The ratio of eluting mass to molar mass provides an accurate estimate of the number of viral particles injected. However, DLS measurements of AAV concentration are similar to those of any other nanoparticle.</p></div></div></div><div class="column mcb-column mcb-item-pln7eplq one-third laptop-one-third tablet-one-third mobile-one column_image" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-pln7eplq mcb-item-image-inner"><div class="image_frame image_item no_link scale-with-grid alignnone no_border" ><div class="image_wrapper "><img width="600" height="600" src="https://www.wyatt.com/wp-content/uploads/2021/06/AAV-600.jpg" class="scale-with-grid" alt="" style="" decoding="async" srcset="https://www.wyatt.com/wp-content/uploads/2021/06/AAV-600.jpg 600w, https://www.wyatt.com/wp-content/uploads/2021/06/AAV-600-300x300.jpg 300w, https://www.wyatt.com/wp-content/uploads/2021/06/AAV-600-150x150.jpg 150w, https://www.wyatt.com/wp-content/uploads/2021/06/AAV-600-146x146.jpg 146w, https://www.wyatt.com/wp-content/uploads/2021/06/AAV-600-50x50.jpg 50w, https://www.wyatt.com/wp-content/uploads/2021/06/AAV-600-75x75.jpg 75w, https://www.wyatt.com/wp-content/uploads/2021/06/AAV-600-85x85.jpg 85w, https://www.wyatt.com/wp-content/uploads/2021/06/AAV-600-80x80.jpg 80w" sizes="(max-width:767px) 480px, 600px" /></div></div> </div></div></div></div><div id="applications" class="wrap mcb-wrap mcb-wrap-o3rbw0nej one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-o3rbw0nej mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-co3npnnbk two-third laptop-two-third tablet-two-third mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-co3npnnbk mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h3>Applications</h3> <p>The size range, convenience and automation inherent in MALS and DLS make them well-suited to determining concentration and characterizing quantitative particle size distributions of nanomedicines and engineered nanoparticles such as:</p> <ul> <li>viruses and viral vectors</li> <li>lipid nanoparticles and liposomes</li> <li>polymersomes, PLGA nanoparticles</li> <li>nanobeads for bioprocessing and nanocages</li> <li>decorated gold nanoparticles</li> </ul> <p>These techniques may be applied in research and discovery, product and process development, production and quality control, in standard labs or GMP environments.</p></div></div></div><div class="column mcb-column mcb-item-ja7ggbp8u one-third laptop-one-third tablet-one-third mobile-one column_image" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-ja7ggbp8u mcb-item-image-inner"><div class="image_frame image_item no_link scale-with-grid alignnone no_border" ><div class="image_wrapper "><img width="360" height="243" src="https://www.wyatt.com/wp-content/uploads/2021/08/DynaPro-Plate-Reader-III-360.png" class="scale-with-grid" alt="The DynaPro Plate Reader measures particle size and concentration in standard flat-bottom 96, 384 or 1536 microwell plates." style="" decoding="async" srcset="https://www.wyatt.com/wp-content/uploads/2021/08/DynaPro-Plate-Reader-III-360.png 360w, https://www.wyatt.com/wp-content/uploads/2021/08/DynaPro-Plate-Reader-III-360-300x203.png 300w, https://www.wyatt.com/wp-content/uploads/2021/08/DynaPro-Plate-Reader-III-360-216x146.png 216w, https://www.wyatt.com/wp-content/uploads/2021/08/DynaPro-Plate-Reader-III-360-50x34.png 50w, https://www.wyatt.com/wp-content/uploads/2021/08/DynaPro-Plate-Reader-III-360-111x75.png 111w" sizes="(max-width:767px) 360px, 360px" /></div><p class="wp-caption-text">The DynaPro Plate Reader measures particle size and concentration in standard flat-bottom 96, 384 or 1536 microwell plates.</p></div> </div></div></div></div><div id="methods" class="wrap mcb-wrap mcb-wrap-7ws5y1umc one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-7ws5y1umc mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-bihbydhfp one laptop-one tablet-one mobile-one column_heading" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-bihbydhfp mcb-item-heading-inner"><h2 class="title">Methods</h2></div></div></div></div><div id="sec-fff-mals" class="wrap mcb-wrap mcb-wrap-2nmqz3mas one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-2nmqz3mas mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div id="sec-fff-mals" class="column mcb-column mcb-item-r1it098qy one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-r1it098qy mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h3>SEC/FFF-MALS</h3> <p>MALS is most often coupled downstream of a size-based separation method such as size-exclusion chromatography (<a href="/solutions/techniques/sec-mals-molar-mass-size-multi-angle-light-scattering.html">SEC-MALS</a>) or field-flow fractionation (<a href="/solutions/techniques/fff-mals-characterization-of-nanoparticles-colloids-macromolecules.html">FFF-MALS</a>). Separation prior to measurement enables high-resolution particle size distributions across a size range of 13 nm to 650 nm in radius (equivalent to rms radius range of 10 nm to 500 nm), where the resolution is limited mainly by the separation method.</p> <p>Because each size fraction is measured individually, the detector is not “blinded” to small particles by strong scattering from large particles so concentration values are generally quite accurate. If particles composed of different materials are separated, each peak may be assigned a corresponding <i>n</i><sub>particle</sub> value. SEC-MALS uses a standard HPLC system with a <a href="/products/instruments/dawn-multi-angle-light-scattering-detector.html">DAWN<sup>™</sup> MALS instrument</a>; FFF-MALS adds an <a href="/products/instruments/eclipse-field-flow-fractionation-system.html">Eclipse<sup>™</sup> FFF controller</a> and separation channel. <a href="/products/software/astra.html">ASTRA<sup>™</sup> software</a> analyzes the particle size and concentration in each eluting fraction, and can integrate peaks to calculate the total number of injected particles in each size range. ASTRA supports <a href="/products/services/21-cfr-part-11-compliance.html">21 CFR Part 11 compliance</a>, as does the <a href="/products/software/vision.html">VISION<sup>™</sup> FFF software</a>.</p> <p>In addition to size and concentration, a setup may be configured with UV, fluorescence and/or refractive index detectors in order to determine the molar mass, composition and payload of many types of nanoparticles.</p> </div></div></div><div class="column mcb-column mcb-item-9ymbiqde three-fifth laptop-three-fifth tablet-three-fifth mobile-one column_image" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-9ymbiqde mcb-item-image-inner"><div class="image_frame image_item no_link scale-with-grid alignnone no_border hover-disable" ><div class="image_wrapper "><img width="750" height="475" src="https://www.wyatt.com/wp-content/uploads/2021/08/methods-fff-mals.jpg" class="scale-with-grid" alt="FFF-MALS is an excellent tool for vaccine characterization. Total viral count and size may be quantified with high resolution for each subpopulation." style="" decoding="async" loading="lazy" srcset="https://www.wyatt.com/wp-content/uploads/2021/08/methods-fff-mals.jpg 750w, https://www.wyatt.com/wp-content/uploads/2021/08/methods-fff-mals-300x190.jpg 300w, https://www.wyatt.com/wp-content/uploads/2021/08/methods-fff-mals-231x146.jpg 231w, https://www.wyatt.com/wp-content/uploads/2021/08/methods-fff-mals-50x32.jpg 50w, https://www.wyatt.com/wp-content/uploads/2021/08/methods-fff-mals-118x75.jpg 118w" sizes="(max-width:767px) 480px, 750px" /></div><p class="wp-caption-text">FFF-MALS is an excellent tool for vaccine characterization. Total viral count and size may be quantified with high resolution for each subpopulation.</p></div> </div></div></div></div><div id="mals-for-pat" class="wrap mcb-wrap mcb-wrap-4ai1mo6bv one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-4ai1mo6bv mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-hcbb1qs3h one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-hcbb1qs3h mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h3>MALS for PAT</h3> <p><a href="/solutions/techniques/real-time-mals.html">Real-time MALS (RT-MALS)</a> measurements of concentration are generally performed without separation in order to provide rapid feedback for process analytical (PAT) applications, reporting results 30 times per minute. The values may be integrated to calculate the final titer of, e.g., virions in the pooled fractions. At the same time, size is determined continuously for use as a biologics DSP pooling criterion or to monitor deviations of synthetic nanoparticles in the course of manufacturing.</p> <p>The <a href="/products/instruments/ultradawn.html">ultraDAWN<sup>™</sup> RT-MALS instrument</a> is usually connected in-line with production or purification systems operating at lower flow rates. For static reactors or high-flow-rate processes, it is configured on-line, where a pump draws some of the product and runs it through the ultraDAWN. <a href="/products/software/observer.html">OBSERVER<sup>™</sup> software</a> orchestrates the entire process; pre-defined trigger conditions may be set to alert the operator upon reaching an end point or deviating from acceptable product attributes.</p> <p>RT-MALS only determines the average size and not a full distribution, so if the size distribution is wide, the particle concentration values may not be accurate. The reported concentration is most reliable for narrowly disperse nanoparticles.</p></div></div></div><div class="column mcb-column mcb-item-7dvzthjm7 three-fifth laptop-three-fifth tablet-three-fifth mobile-one column_image" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-7dvzthjm7 mcb-item-image-inner"><div class="image_frame image_item no_link scale-with-grid alignnone no_border hover-disable" ><div class="image_wrapper "><img width="1000" height="667" src="https://www.wyatt.com/wp-content/uploads/2021/08/ultraDAWN-graph-1000.jpg" class="scale-with-grid" alt="RT-MALS monitors particle concentration in the course of tangential flow filtration. A trigger upon reaching the specified end point. The ultraDAWN simultaneously measures particle size to assess virion degradation." style="" decoding="async" loading="lazy" srcset="https://www.wyatt.com/wp-content/uploads/2021/08/ultraDAWN-graph-1000.jpg 1000w, https://www.wyatt.com/wp-content/uploads/2021/08/ultraDAWN-graph-1000-300x200.jpg 300w, https://www.wyatt.com/wp-content/uploads/2021/08/ultraDAWN-graph-1000-768x512.jpg 768w, https://www.wyatt.com/wp-content/uploads/2021/08/ultraDAWN-graph-1000-219x146.jpg 219w, https://www.wyatt.com/wp-content/uploads/2021/08/ultraDAWN-graph-1000-50x33.jpg 50w, https://www.wyatt.com/wp-content/uploads/2021/08/ultraDAWN-graph-1000-112x75.jpg 112w" sizes="(max-width:767px) 480px, (max-width:1000px) 100vw, 1000px" /></div><p class="wp-caption-text">RT-MALS monitors particle concentration in the course of tangential flow filtration. A trigger upon reaching the specified end point. The ultraDAWN simultaneously measures particle size to assess virion degradation.</p></div> </div></div></div></div><div id="dls" class="wrap mcb-wrap mcb-wrap-0a129a28h one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-0a129a28h mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-am7p673vo one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-am7p673vo mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h3>DLS</h3> <p>DLS is usually performed without separation, in microcuvettes or microwell plates. This “batch” mode operation enables fast measurements of relatively low-resolution distributions, with less accuracy than SEC-MALS or FFF-MALS but potentially more than RT-MALS. High-throughput DLS in microwell plates is ideal for analyzing viral vector downstream bioprocess fractions or a nanoparticle formulation matrix.</p> <p>The size range (hydrodynamic radius) over which concentration may be measured is 0.5 nm to 160 nm in the <a href="/products/instruments/dynapro-dynamic-light-scattering-plate-reader.html">DynaPro<sup>™</sup> Plate Reader</a> and 0.2 nm to 175 nm in the <a href="/products/instruments/dynapro-nanostar-dynamic-light-scattering-detector.html">DynaPro<sup>™</sup> NanoStar<sup>™</sup></a>, making it suitable for most viruses and nanomedicines as well as other types of nanoparticles. If more than one size population is evident in the distribution, <a href="/products/software/dynamics.html">DYNAMICS<sup>™</sup> software</a> will provide the concentration of each.</p></div></div></div><div class="column mcb-column mcb-item-15iakio1h three-fifth laptop-three-fifth tablet-three-fifth mobile-one column_image" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-15iakio1h mcb-item-image-inner"><div class="image_frame image_item no_link scale-with-grid alignnone no_border hover-disable" ><div class="image_wrapper "><img width="800" height="533" src="https://www.wyatt.com/wp-content/uploads/2021/08/DLS-Graph.jpg" class="scale-with-grid" alt="Size distribution obtained by DLS showing the mean size of two populations and the concentration of each." style="" decoding="async" loading="lazy" srcset="https://www.wyatt.com/wp-content/uploads/2021/08/DLS-Graph.jpg 800w, https://www.wyatt.com/wp-content/uploads/2021/08/DLS-Graph-300x200.jpg 300w, https://www.wyatt.com/wp-content/uploads/2021/08/DLS-Graph-768x512.jpg 768w, https://www.wyatt.com/wp-content/uploads/2021/08/DLS-Graph-219x146.jpg 219w, https://www.wyatt.com/wp-content/uploads/2021/08/DLS-Graph-50x33.jpg 50w, https://www.wyatt.com/wp-content/uploads/2021/08/DLS-Graph-113x75.jpg 113w" sizes="(max-width:767px) 480px, (max-width:800px) 100vw, 800px" /></div><p class="wp-caption-text">Size distribution obtained by DLS showing the mean size of two populations and the concentration of each.</p></div> </div></div><div class="column mcb-column mcb-item-gac8wtzos one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-gac8wtzos mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""> <p>Wyatt’s DLS instruments dramatically minimize sample requirements. Only 2 µL of sample are required in quartz cuvettes, and in the most common 384 well plates, 20 µL are needed for optimal measurements. Though these instruments cover several orders of magnitude in concentration, if particle concentrations are high and dilution is necessary, it is easy to do so directly in the measurement volume.</p></div></div></div></div></div><div id="dls-in-qc-and-at-line-pat" class="wrap mcb-wrap mcb-wrap-myc1ci4ln one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-myc1ci4ln mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-kyrbgxral one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-kyrbgxral mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h3>DLS in QC and at-line PAT</h3></div></div></div><div class="column mcb-column mcb-item-5ph6wsafd three-fifth laptop-three-fifth tablet-three-fifth mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-5ph6wsafd mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><p>DLS is especially useful for quick and easy nanoparticle analysis with minimal instrument complexity or sample prep effort. Wyatt’s DLS instruments are supported by <a href="/products/software/dynamics.html">DYNAMICS<sup>™</sup> software</a>, a comprehensive suite of instrument control, data acquisition and analysis that provides extensive automation, rich characterization capabilities and intuitive feedback on the quality and reliability of each and every measurement.</p> <p>For use in quality control and at-line process analytics, DYNAMICS offer <a href="/products/services/21-cfr-part-11-compliance.html">21 CFR Part 11 compliance</a>. In addition, it supports an application programming interface (API) that enables integration with third-party liquid handlers and high-level process or quality control software.</p></div></div></div><div class="column mcb-column mcb-item-ph2zrviqn two-fifth laptop-two-fifth tablet-two-fifth mobile-one column_image" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-ph2zrviqn mcb-item-image-inner"><div class="image_frame image_item no_link scale-with-grid alignnone no_border" ><div class="image_wrapper "><img width="825" height="422" src="https://www.wyatt.com/wp-content/uploads/2020/03/Researcher-Pipetting-825.jpg" class="scale-with-grid" alt="" style="" decoding="async" loading="lazy" srcset="https://www.wyatt.com/wp-content/uploads/2020/03/Researcher-Pipetting-825.jpg 825w, https://www.wyatt.com/wp-content/uploads/2020/03/Researcher-Pipetting-825-300x153.jpg 300w, https://www.wyatt.com/wp-content/uploads/2020/03/Researcher-Pipetting-825-768x393.jpg 768w, https://www.wyatt.com/wp-content/uploads/2020/03/Researcher-Pipetting-825-260x133.jpg 260w, https://www.wyatt.com/wp-content/uploads/2020/03/Researcher-Pipetting-825-50x26.jpg 50w, https://www.wyatt.com/wp-content/uploads/2020/03/Researcher-Pipetting-825-147x75.jpg 147w" sizes="(max-width:767px) 480px, (max-width:825px) 100vw, 825px" /></div></div> </div></div></div></div><div id="additional-resources" class="wrap mcb-wrap mcb-wrap-k4nfn57x7 one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-k4nfn57x7 mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-irjirwuid one laptop-one tablet-one mobile-one column_heading" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-irjirwuid mcb-item-heading-inner"><h2 class="title">Additional Resources</h2></div></div></div></div><div id="selected-resources" class="wrap mcb-wrap mcb-wrap-kvmp9an9z one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-kvmp9an9z mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-gz207x20d one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-gz207x20d mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h3>Selected Resources</h3> <p>Webinar - <a href="/library/webinars/virtual-user-meeting-june-2020.html" target="_blank" rel="noopener">The Light Scattering Toolbox: Measuring Particle Concentration</a></p> <p>Ask the Expert - <a href="https://www.wyatt.com/ask-the-expert/how-do-i-determine-particle-concentrations-for-multimodal-samples-in-dynamics.html" target="_blank" rel="noopener">How do I determine particle concentrations for multimodal samples in DYNAMICS?</a></p> <div style="display: flex; align-items: center; gap: 8px; margin-bottom: 10px;"><i class="fas fa-file-pdf" style="color: #8a8a8d; font-size: 22px;"></i> <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/AAV-Application-Notebook.pdf" target="_blank" rel="noopener">Application Note: AAV Application Notebook</a></div> <div style="display: flex; align-items: center; gap: 8px; margin-bottom: 10px;"><i class="fa-solid fa-arrow-up-right-from-square" style="color: #8a8a8d; font-size: 16px; opacity: 0.7;"></i> <a href="/library/application-notes/build-a-search.html" target="_blank" rel="noopener">More Applications</a></div></div></div></div></div></div><div id="selected-references" class="wrap mcb-wrap mcb-wrap-z11t62yoj one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-z11t62yoj mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-zld7gw8vb one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-zld7gw8vb mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h3>Selected References</h3> <p>Improved multidetector-asymmetrical flow field flow fractionation method for particle sizing and concentration measurements of lipid-based nanocarriers for RNA delivery, Mildner, R. et al. <i>Eu. J. Pharm. Biopharm.</i> <b>163</b>, 252-265 (2021) <a href="https://doi.org/10.1016/j.ejpb.2021.03.004" target="blank">https://doi.org/10.1016/j.ejpb.2021.03.004</a></p> <p>Biophysical characterization of influenza virus subpopulations using field flow fractionation and multiangle light scattering: correlation of particle counts, size distribution and infectivity, Wei, Z. et al <i>J. Virolog. Meth.</i> <b>144</b>(1-2), 122-132 (2007). <a href="https://doi.org/10.1016/j.jviromet.2007.04.008" target="blank">https://doi.org/10.1016/j.jviromet.2007.04.008</a></p> <p>Quantitation of influenza virus using field-flow fractionation and multi-angle light scattering for quantifying influenza A particles, Bousse, T. et al. <i>J. Virolog. Meth.</i> <b>193</b>(2), 589-596 (2013). <a href="https://doi.org/10.1016/j.jviromet.2013.07.026" target="blank">https://doi.org/10.1016/j.jviromet.2013.07.026</a></p> <p>Quantification and characterization of virus-like particles by size-exclusion chromatography and nanoparticle tracking analysis, Steppert, P. et al. <i>J. Chromat. A</i> <b>1487</b>, 89-99 (2017) <a href="https://dx.doi.org/10.1016/j.chroma.2016.12.085 " target="blank">http://dx.doi.org/10.1016/j.chroma.2016.12.085 </a></p> <p><a href="/library/bibliography.html" target="_blank" class="sidebarLink">BIBLIOGRAPHIC SEARCH</a></p></div></div></div></div></div></div></div></div></section></div><section class="section mcb-section the_content no_content"><div class="section_wrapper"><div class="the_content_wrapper "></div></div></section> <section class="section section-page-footer"> <div class="section_wrapper clearfix"> <div class="column one page-pager"> <div class="mcb-column-inner"> </div> </div> </div> </section> </div> </main> </div> </div>  <section class="section before-footer mcb-section mfn-default-section mcb-section-arexmxc3 default-width" style="padding-top:70px;"><div class="mcb-background-overlay"></div><div class="section_wrapper mfn-wrapper-for-wraps mcb-section-inner mcb-section-inner-arexmxc3"><div class="wrap mcb-wrap 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