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leishmaniasis</title> <meta name="description" content="Search results for: leishmaniasis"> <meta name="keywords" content="leishmaniasis"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="leishmaniasis"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 41</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: leishmaniasis</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">41</span> Treatment Outcome of Cutaneous Leishmaniasis and Its Associated Factors among Admitted Patients in All Africa Leprosy Rehabilitation and Training Center Hospital, Ethiopia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kebede%20Mairie">Kebede Mairie</a>, <a href="https://publications.waset.org/abstracts/search?q=Getahun%20Belete"> Getahun Belete</a>, <a href="https://publications.waset.org/abstracts/search?q=Mitike%20Abeba"> Mitike Abeba</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Leishmania aethiopica is a peculiar parasite causing cutaneous leishmaniasis in Ethiopia and its mainstay treatment is Sodium Stibogluconate. However, its treatment outcome in Ethiopia is not well documented. Objectives: To determine the treatment outcome of admitted cutaneous leishmaniasis patients and its associated factors in Addis Ababa, Ethiopia. Methods: A retrospective study was conducted from 1st November 2021 to 30th March 2022. Medical records of all cutaneous leishmaniasis-diagnosed and admitted patients who received parenteral sodium stibogluconate at All Africa Leprosy Rehabilitation and Training Center (ALERT) hospital, the main Leishmania treatment center in Ethiopia from July 2011 to September 2021 were reviewed. Results: A total of 827 charts of admitted cases from July 2011 to September 2021 were retrieved, but 667 (80.65%) were reviewed. Improvement in the treatment outcome was recorded in 93.36 % in the first course of SSG treatment and 96.23%, 94.62%, and 96.97% subsequently in the second, third and fourth treatment courses, respectively. Female gender and diffuse cutaneous leishmaniasis were the two predictive determinants in the treatment of cutaneous leishmaniasis. Conclusion: The study shows that parenteral sodium stibogluconate therapy treats hospitalized cutaneous leishmaniasis patients well, with female gender and diffuse cutaneous leishmaniasis having poor outcomes suggesting the need for a different approach for diffuse cutaneous leishmaniasis patients. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cutaneous%20leishmaniasis" title="cutaneous leishmaniasis">cutaneous leishmaniasis</a>, <a href="https://publications.waset.org/abstracts/search?q=leishmania%20aethiopica" title=" leishmania aethiopica"> leishmania aethiopica</a>, <a href="https://publications.waset.org/abstracts/search?q=sodium%20stibogluconate" title=" sodium stibogluconate"> sodium stibogluconate</a>, <a href="https://publications.waset.org/abstracts/search?q=diffuse%20cutaneous%20leishmaniasis" title=" diffuse cutaneous leishmaniasis"> diffuse cutaneous leishmaniasis</a>, <a href="https://publications.waset.org/abstracts/search?q=pentostam" title=" pentostam"> pentostam</a> </p> <a href="https://publications.waset.org/abstracts/164278/treatment-outcome-of-cutaneous-leishmaniasis-and-its-associated-factors-among-admitted-patients-in-all-africa-leprosy-rehabilitation-and-training-center-hospital-ethiopia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/164278.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">77</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">40</span> The Clinical Significance of Cutaneous Leishmaniasis in Immigrant and Refugee Populations</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Promise%20Ufomadu">Promise Ufomadu</a>, <a href="https://publications.waset.org/abstracts/search?q=Edgar%20Rodriguez"> Edgar Rodriguez</a>, <a href="https://publications.waset.org/abstracts/search?q=Grace%20Lee"> Grace Lee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cutaneous Leishmaniasis (CL) is an infection caused by a variety of Leishmania species which are protozoan organisms that are typically carried by sandflies found in tropical regions. The parasite causes skin lesions that may resolve spontaneously but commonly become chronic and therefore necessitate thorough clinical attention. We present a 15-year-old female patient with CL of her bilateral dorsal hands, which resolved after a 28-day course of miltefosine. This case details the significance of compiling a thorough patient history and considering CL as a possible differential in patients from endemic regions. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=leishmaniasis" title="leishmaniasis">leishmaniasis</a>, <a href="https://publications.waset.org/abstracts/search?q=infection" title=" infection"> infection</a>, <a href="https://publications.waset.org/abstracts/search?q=immigrant" title=" immigrant"> immigrant</a>, <a href="https://publications.waset.org/abstracts/search?q=parasites" title=" parasites"> parasites</a>, <a href="https://publications.waset.org/abstracts/search?q=pediatrics" title=" pediatrics"> pediatrics</a> </p> <a href="https://publications.waset.org/abstracts/168690/the-clinical-significance-of-cutaneous-leishmaniasis-in-immigrant-and-refugee-populations" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/168690.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">86</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">39</span> Attitude and Knowledge of Primary Health Care Physicians and Local Inhabitants about Leishmaniasis and Sandfly in West Alexandria, Egypt</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Randa%20M.%20Ali">Randa M. Ali</a>, <a href="https://publications.waset.org/abstracts/search?q=Naguiba%20F.%20Loutfy"> Naguiba F. Loutfy</a>, <a href="https://publications.waset.org/abstracts/search?q=Osama%20M.%20Awad"> Osama M. Awad</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Leishmaniasis is a worldwide disease, affecting 88 countries, it is estimated that about 350 million people are at risk of leishmaniasis. Overall prevalence is 12 million people with annual mortality of about 60,000. Annual incidence is 1,500,000 cases of cutaneous leishmaniasis (CL) worldwide and half million cases of visceral Leishmaniasis (VL). Objectives: The objective of this study was to assess primary health care physicians knowledge (PHP) and attitude about leishmaniasis and to assess awareness of local inhabitants about the disease and its vector in four areas in west Alexandria, Egypt. Methods: This study was a cross sectional survey that was conducted in four PHC units in west Alexandria. All physicians currently working in these units during the study period were invited to participate in the study, only 20 PHP completed the questionnaire. 60 local inhabitant were selected randomly from the four areas of the study, 15 from each area; Data was collected through two different specially designed questionnaires. Results: 11(55%) percent of the physicians had satisfactory knowledge, they answered more than 9 (60%) questions out of a total 14 questions about leishmaniasis and sandfly. The second part of the questionnaire is concerned with attitude of the primary health care physicians about leishmaniasis, 17 (85%) had good attitude and 3 (15%) had poor attitude. The second questionnaire showed that the awareness of local inhabitants about leishmaniasis and sandly as a vector of the disease is poor and needs to be corrected. Most of the respondents (90%) had not heard about leishmaniasis, Only 3 (5%) of the interviewed inhabitants said they know sandfly and its role in transmission of leishmaniasis. Conclusions: knowledge and attitudes of physicians are acceptable. However, there is, room for improvement and could be done through formal training courses and distribution of guidelines. In addition to raising the awareness of primary health care physicians about the importance of early detection and notification of cases of lesihmaniasis. Moreover, health education for raising awareness of the public regarding the vector and the disease is necessary because related studies have demonstrated that if the inhabitants do not perceive mosquitoes to be responsible for diseases such as malaria they do not take enough measures to protect themselves against the vector. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=leishmaniasis" title="leishmaniasis">leishmaniasis</a>, <a href="https://publications.waset.org/abstracts/search?q=PHP" title=" PHP"> PHP</a>, <a href="https://publications.waset.org/abstracts/search?q=knowledge" title=" knowledge"> knowledge</a>, <a href="https://publications.waset.org/abstracts/search?q=attitude" title=" attitude"> attitude</a>, <a href="https://publications.waset.org/abstracts/search?q=local%20inhabitants" title=" local inhabitants "> local inhabitants </a> </p> <a href="https://publications.waset.org/abstracts/12442/attitude-and-knowledge-of-primary-health-care-physicians-and-local-inhabitants-about-leishmaniasis-and-sandfly-in-west-alexandria-egypt" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/12442.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">449</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">38</span> Epidemiology, Prevention and Treatment of Leishmaniasis in Afghanistan</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20Reza%20Mohammadi">Mohammad Reza Mohammadi</a>, <a href="https://publications.waset.org/abstracts/search?q=Layegheh%20Daliri"> Layegheh Daliri</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Leishmaniasis occurs in infectious diseases of Leishmania protozoa in Afghanistan, anthroponotic leishmaniasis and common cutaneous leishmaniasis (ZCL). Anthroponotic skin leishmania tropica may cause urban diseases and transmitted by Phlebotomus Sergenti. In different parts of Afghanistan, different species of Leishmania are observed. We report the epidemiological characteristics of prevention and treatment in this study. Methods: This study examines the epidemiology and prevention of religious diseases in Afghanistan. Knowledge gaps were analyzed and collected with our own data. Results: In Afghanistan, most of the Lishmania Tropic seekers are Four species of Leishmania in northern Afghanistan, including Leishmania Tropica, L. Major and L. Donovani, cause skin lesions, but L. Donovani and L. infantum are visible. Even combined prevention can significantly reduce the amount of infection. Conclusion: Skinny, as well as visceral leishmaniasis, can occur among the returnees from Afghanistan. Unusual and poor skin lesions can be created by L. Donovani. In most pathogenic areas, the transmission of common diseases between humans and animals. Home dogs are the main reservoir, transferring in some areas such as India and Sudan. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=leishmania%20donovani" title="leishmania donovani">leishmania donovani</a>, <a href="https://publications.waset.org/abstracts/search?q=leishmania%20tropica" title=" leishmania tropica"> leishmania tropica</a>, <a href="https://publications.waset.org/abstracts/search?q=treatment" title=" treatment"> treatment</a>, <a href="https://publications.waset.org/abstracts/search?q=disease" title=" disease"> disease</a>, <a href="https://publications.waset.org/abstracts/search?q=epidemiology" title=" epidemiology"> epidemiology</a> </p> <a href="https://publications.waset.org/abstracts/142585/epidemiology-prevention-and-treatment-of-leishmaniasis-in-afghanistan" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/142585.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">182</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">37</span> Separation, Identification, and Measuring Gossypol in the Cottonseed Oil and Investigating the Performance of Drugs Prepared from the Combination of Plant Extract and Oil in the Treatment of Cutaneous Leishmaniasis Resistant to Drugs</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sara%20Taghdisi">Sara Taghdisi</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Mirmohammadi"> M. Mirmohammadi</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Mokhtarian"> M. Mokhtarian</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In 2013, the World Health Organization announced the cases of Cutaneous leishmaniasis infection in Iran between 69,000 to 113,000. The most common chemical drugs for Cutaneous leishmaniasis treatment are sodium stibogluconate, and meglumine antimonate, which not only have relatively many side effects, but also some species of the Leishmania genus have become resistant to them .The most prominent compound existing in different parts of the cotton plant is a yellow polyphenol called Gossypol. Gossypol is an extremely valuable compound and has anti-cancer properties. In the current project, Gossypol was extracted with a liquid-liquid extraction method in 120 minutes in the presence of Phosphoric acid from the cotton seed oil of Golestan beach varieties, then got crystallized in darkness using Acetic acid and isolated as Gossypol Acetic acid. The efficiency of the extracted crystal was obtained at 0.12+- 1.28. the cotton plant could be efficient in the treatment of Cutaneous leishmaniasis. The extract of the green-leaf cotton boll of Jargoyeh varieties was tested as an ointment on the target group of patients suffering from Cutaneous leishmaniasis resistant to drugs esistant to drugs by our colleagues in the research team. The results showed the Pearson's correlation coefficient of 0.72 between the two variables of wound diameter and the extract use over time which indicated the positive effect of this extract on the treatment of Cutaneous leishmaniasis was resistant to drugs. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cottonseed%20oil" title="cottonseed oil">cottonseed oil</a>, <a href="https://publications.waset.org/abstracts/search?q=crystallization" title=" crystallization"> crystallization</a>, <a href="https://publications.waset.org/abstracts/search?q=gossypol" title=" gossypol"> gossypol</a>, <a href="https://publications.waset.org/abstracts/search?q=green-leaf" title=" green-leaf"> green-leaf</a> </p> <a href="https://publications.waset.org/abstracts/170536/separation-identification-and-measuring-gossypol-in-the-cottonseed-oil-and-investigating-the-performance-of-drugs-prepared-from-the-combination-of-plant-extract-and-oil-in-the-treatment-of-cutaneous-leishmaniasis-resistant-to-drugs" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/170536.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">109</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">36</span> An Optimal Control Model for the Dynamics of Visceral Leishmaniasis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ibrahim%20M.%20Elmojtaba">Ibrahim M. Elmojtaba</a>, <a href="https://publications.waset.org/abstracts/search?q=Rayan%20M.%20Altayeb"> Rayan M. Altayeb</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Visceral leishmaniasis (VL) is a vector-borne disease caused by the protozoa parasite of the genus leishmania. The transmission of the parasite to humans and animals occurs via the bite of adult female sandflies previously infected by biting and sucking blood of an infectious humans or animals. In this paper we use a previously proposed model, and then applied two optimal controls, namely treatment and vaccination to that model to investigate optimal strategies for controlling the spread of the disease using treatment and vaccination as the system control variables. The possible impact of using combinations of the two controls, either one at a time or two at a time on the spread of the disease is also examined. Our results provide a framework for vaccination and treatment strategies to reduce susceptible and infection individuals of VL in five years. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=visceral%20leishmaniasis" title="visceral leishmaniasis">visceral leishmaniasis</a>, <a href="https://publications.waset.org/abstracts/search?q=treatment" title=" treatment"> treatment</a>, <a href="https://publications.waset.org/abstracts/search?q=vaccination" title=" vaccination"> vaccination</a>, <a href="https://publications.waset.org/abstracts/search?q=optimal%20control" title=" optimal control"> optimal control</a>, <a href="https://publications.waset.org/abstracts/search?q=numerical%20simulation" title=" numerical simulation"> numerical simulation</a> </p> <a href="https://publications.waset.org/abstracts/39487/an-optimal-control-model-for-the-dynamics-of-visceral-leishmaniasis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/39487.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">404</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">35</span> Isolation, Identification and Measurement of Cottonseed Oil Gossypol in the Treatment of Drug-Resistant Cutaneous Leishmaniasis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sara%20Taghdisi">Sara Taghdisi</a>, <a href="https://publications.waset.org/abstracts/search?q=Mehrosadat%20Mirmohammadi"> Mehrosadat Mirmohammadi</a>, <a href="https://publications.waset.org/abstracts/search?q=Mostafa%20Mokhtarian"> Mostafa Mokhtarian</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20Hossein%20Pazandeh"> Mohammad Hossein Pazandeh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Leishmaniasis is one of the 10 most important diseases of the World Health Organization with health problems in more than 90 countries. Over one billion people are at risk of these diseases on almost every continent. The present human study was performed to evaluate the therapeutic effect of cotton plant on cutaneous leishmaniasis leision. firstly, the cotton seeds were cleaned and grinded to smaller particles. In the second step, the seeds were oiled by cold press method. In order to separate bioactive compound, after saponification of the oil, its gossypol was hydrolyzed and crystalized. finally, the therapeutic effect of Cottonseed Oil on cutaneous leishmaniasis was investigated. In the current project, Gossypol was extracted with a liquid-liquid extraction method in 120 minutes in the presence of Phosphoric acid from the cotton seed oil of Golestan beach varieties, then got crystallized in darkness using Acetic acid and isolated as Gossypol Acetic acid. The efficiency of the extracted crystal was obtained at 1.28±0.12. the cotton plant could be efficient in the treatment of Cutaneous leishmaniasis. This double-blind randomized controlled clinical trial was performed on 88 cases of leishmaniasis wounds. Patients were randomly divided into two groups of 44 cases. two groups received conventional treatment. In addition to the usual treatment (glucantime), the first group received cottonseed oil and the control group received placebo. The results of the present study showed that the surface of lesion before the intervention and in the first to fourth weeks after the intervention was not significantly different between the two groups (P-value> 0.05). But the surface of lesion in the Intervention group in the eighth and twelfth weeks was lower than the control group (P-value <0.05). This study showed that the improvement of leishmaniasis lesion using topical cotton plant mark in the eighth and twelfth weeks after the intervention was significantly more than the control group. Considering the most common chemical drugs for Cutaneous leishmaniasis treatment are sodium stibogluconate, and meglumine antimonate, which not only have relatively many side effects, but also some species of the Leishmania genus have become resistant to them. Therefore, a plant base bioactive compound such as cottonseed oil can be useful whit fewer side effects. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cottonseed%20oil" title="cottonseed oil">cottonseed oil</a>, <a href="https://publications.waset.org/abstracts/search?q=crystallization" title=" crystallization"> crystallization</a>, <a href="https://publications.waset.org/abstracts/search?q=gossypol" title=" gossypol"> gossypol</a>, <a href="https://publications.waset.org/abstracts/search?q=leishmaniasis" title=" leishmaniasis"> leishmaniasis</a> </p> <a href="https://publications.waset.org/abstracts/184861/isolation-identification-and-measurement-of-cottonseed-oil-gossypol-in-the-treatment-of-drug-resistant-cutaneous-leishmaniasis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/184861.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">61</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">34</span> Prevalence of Cutaneous Leishmaniasis in Human Population of District Kurram, Khyber Pakhtunkhwa, Pakistan</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shah%20Abid">Shah Abid</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Leishmaniasis is a collection of zoonotic infections that affect the viscera, skin, and mucous membrane and are spread by several species of female sandflies in the subfamily phlebotominae. The study's goal was to assess the prevalence of Cutaneous Leishmaniasis in District Kurram using descriptive and cross-sectional methods. From December 2022 to May 2023, the study was carried out at Tehsil Head Quarter (THQ) Hospital, Sadda, District Kurram in the Dermatology Department. The disease was identified using a laboratory method based on clinical manifestations of leishmaniasis. A clean slide's surface was applied to the scraped-off portion of the lesions and rubbed over the blood to make a smear on the slide. The slides were methanol-fixed, stained with traditional Giemsa, and meticulously examined at high magnification to search for LD bodies. The necessary information, such as residence area, lesion kind and location, age, sex, and the total number of lesions, was meticulously acquired. During the time of the investigation, 393 instances of cutaneous leishmaniasis were observed. 1 year to 70 years old was the age range (mean age: 35.45). The age group that was most severely impacted, 16 years and older, had 23 (11.67%) children with this condition. Male to female ratio was 9.7:10. Most of the cases (n=52, 26.29%), were reported in the month of May. Majority of the patients 102 (51.77%) had lesion on face. 42 (16.73) patients had multiple lesions on their body. Face was the most common site followed by lower limbs 93 (37.05). Weekly intralesional injections of sodium stibogluconate (glucantime) were administered to all patients. Without any noticeable adverse effects, all patients had positive responses to the treatment. The condition affects adults more commonly than children, according to analysis of the combined results, and it is more common in women than in men. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=District%20Kurram" title="District Kurram">District Kurram</a>, <a href="https://publications.waset.org/abstracts/search?q=cutaneous%20leishmaniasis" title=" cutaneous leishmaniasis"> cutaneous leishmaniasis</a>, <a href="https://publications.waset.org/abstracts/search?q=zoonosis" title=" zoonosis"> zoonosis</a>, <a href="https://publications.waset.org/abstracts/search?q=glucantime" title=" glucantime"> glucantime</a> </p> <a href="https://publications.waset.org/abstracts/193843/prevalence-of-cutaneous-leishmaniasis-in-human-population-of-district-kurram-khyber-pakhtunkhwa-pakistan" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/193843.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">9</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">33</span> Genomic Analysis of Whole Genome Sequencing of Leishmania Major</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Fatimazahrae%20Elbakri">Fatimazahrae Elbakri</a>, <a href="https://publications.waset.org/abstracts/search?q=Azeddine%20Ibrahimi"> Azeddine Ibrahimi</a>, <a href="https://publications.waset.org/abstracts/search?q=Meryem%20Lemrani"> Meryem Lemrani</a>, <a href="https://publications.waset.org/abstracts/search?q=Dris%20Belghyti"> Dris Belghyti</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Leishmaniasis represents a major public health problem because of the number of cases recorded each year and the wide distribution of the disease. It is a parasitic disease of flagellated protozoa transmitted by the bite of certain species of sandfly, causing a spectrum of clinical pathology in humans ranging from disfiguring skin lesions to fatal visceral leishmaniasis. Cutaneous leishmaniasis due to Leishmania major is a polymorphic disease; in fact, the infection can be asymptomatic, localized, or disseminated. The objective of this work is to determine the genomic diversity that contributes to clinical variability by trying to identify the variation in chromosome number and to extract SNPs and SNPs and InDels; it is based on four sequences (WGS) of Leishmania major available on NCBI in Fastq form, from three countries: Tunisia, Algeria, and Israel, the analysis is set up from a pipeline to facilitate the discovery of genetic diversity, in particular SNP and chromosomal somy. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Leshmania%20major" title="Leshmania major">Leshmania major</a>, <a href="https://publications.waset.org/abstracts/search?q=cutaneous%20Leishmania" title=" cutaneous Leishmania"> cutaneous Leishmania</a>, <a href="https://publications.waset.org/abstracts/search?q=NGS" title=" NGS"> NGS</a>, <a href="https://publications.waset.org/abstracts/search?q=genomic" title=" genomic"> genomic</a>, <a href="https://publications.waset.org/abstracts/search?q=somy" title=" somy"> somy</a>, <a href="https://publications.waset.org/abstracts/search?q=variant%20calling" title=" variant calling"> variant calling</a> </p> <a href="https://publications.waset.org/abstracts/170783/genomic-analysis-of-whole-genome-sequencing-of-leishmania-major" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/170783.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">79</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">32</span> Canine Visceral Leishmaniasis In Brazil</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Elisangela%20Sobreira">Elisangela Sobreira</a>, <a href="https://publications.waset.org/abstracts/search?q=Denise%20Teixeira"> Denise Teixeira</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Visceral leishmaniasis is a public health problem in Brazil, it is the main reservoir dog. In the period 2012-2016 78 diagnoses were performed in dogs suspected. Blood samples were collected from the cephalic vein obtaining serum used for the indirect immunofluorescence test and enzyme-linked immunosorbent assay, while it collected a drop of blood for the rapid chromatographic immunoassay. Obtained in 32 dogs positive. The test is important for the control of this disease and is used routinely in the Zoonoses Control Center. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Brazil" title="Brazil">Brazil</a>, <a href="https://publications.waset.org/abstracts/search?q=dogs" title=" dogs"> dogs</a>, <a href="https://publications.waset.org/abstracts/search?q=Leismaniasis" title=" Leismaniasis"> Leismaniasis</a>, <a href="https://publications.waset.org/abstracts/search?q=Zoonoses%20center" title=" Zoonoses center"> Zoonoses center</a> </p> <a href="https://publications.waset.org/abstracts/58774/canine-visceral-leishmaniasis-in-brazil" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/58774.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">262</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">31</span> Refractory Visceral Leishmaniasis Responding to Second-Line Therapy</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Preet%20Shah">Preet Shah</a>, <a href="https://publications.waset.org/abstracts/search?q=Om%20Shrivastav"> Om Shrivastav</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction : In India, Leishmania donovani is the only parasite causing Leishmaniasis. The parasite infects the reticuloendothelial system and is found in the bone marrow, spleen and liver. Treatment of choice is amphotericin-B with sodium stibogluconate being an alternative. Miltefosine is useful in refractory cases. In our case, Leishmaniasis occurred in a person residing in western India (which is quite rare) and it failed to respond to two different drugs (again an uncommon feature) before it finally responded to a third one. Description: A 50 year old lady, a resident of western India, with no history of recent travel, presented with an ulcer on the left side of the nose since 8 months. She was apparently alright 8 months back, when she noticed a small ulcerated lesion on the left ala of the nose which was immediately biopsied. The biopsy revealed amastigotes of Leishmania for which she was administered intra-lesional sodium stibogluconate for 1 month (4 doses every 8 days).Despite this, there was no regression of the ulcer and hence she presented to us for further management. On examination, her vital parameters were normal. Barring an ulcer on the left side of the nose, rest of the examination findings were unremarkable. Complete blood count was normal. Ultrasound abdomen showed hepatomegaly. PET-CT scan showed increased metabolic activity in left ala of nose, hepatosplenomegaly and increased metabolic activity in spleen and bone marrow. Bone marrow biopsy was done which showed hypercellular marrow with erythroid preponderance. Considering a diagnosis of leishmaniasis which had so far been unresponsive to sodium stibogluconate, she was started on liposomal amphotericin-B. At the time of admission, her creatinine level was normal, but it started rising with the administration of liposomal amphotericin-B, hence the dose was reduced. Despite this, creatinine levels did not improve, and she started developing hypokalemia and hypomagnesemia as side effects of the drug, hence further reductions in the dosage were made. Despite a total of 3 weeks of liposomal amphotericin-B, there was no improvement in the ulcer. As had so far failed to respond to sodium stibogluconate and liposomal amphotericin-B, it was decided to start her on miltefosine. She received the miltefosine for a total of 12 weeks. At the end of this duration, there was a marked regression of the cutaneous lesion. Conclusion: Refractoriness to amphotericin-B in leishmaniasis may be seen in up to 5 % cases. Here, an alternative drug such as miltefosine is useful and hence we decided to use it, to which she responded adequately. Furthermore, although leishmaniasis is common in the eastern part of India, it is a relatively unknown entity in the western part of the country with the occurrence being very rare. Because of these 2 reasons, we consider our case to be a unique one. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=amphotericin-b" title="amphotericin-b">amphotericin-b</a>, <a href="https://publications.waset.org/abstracts/search?q=leishmaniasis" title=" leishmaniasis"> leishmaniasis</a>, <a href="https://publications.waset.org/abstracts/search?q=miltefosine" title=" miltefosine"> miltefosine</a>, <a href="https://publications.waset.org/abstracts/search?q=tropical%20diseases" title=" tropical diseases"> tropical diseases</a> </p> <a href="https://publications.waset.org/abstracts/96027/refractory-visceral-leishmaniasis-responding-to-second-line-therapy" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/96027.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">139</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">30</span> Utility of the Loop-Mediated Isothermal Amplification Assay for the Diagnosis of Visceral Leishmaniasis from Blood Samples in Ethiopia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dawit%20Gebreegzabher%20Hagos">Dawit Gebreegzabher Hagos</a>, <a href="https://publications.waset.org/abstracts/search?q=Yazezew%20Kebede%20Kiro"> Yazezew Kebede Kiro</a>, <a href="https://publications.waset.org/abstracts/search?q=Mahmud%20Abdulkader"> Mahmud Abdulkader</a>, <a href="https://publications.waset.org/abstracts/search?q=Henk%20H.%20D.%20F.%20Schallig"> Henk H. D. F. Schallig</a>, <a href="https://publications.waset.org/abstracts/search?q=Dawit%20Wolday"> Dawit Wolday</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Rapid and accurate visceral leishmaniasis (VL) diagnosis is needed to initiate prompt treatment to reduce morbidity and mortality. Here, we evaluated the performance of loop-mediated isothermal amplification (LAMP) assay for the diagnosis of VL from blood in an endemic area in Ethiopia. LAMP was positive in 117/122 confirmed VL cases and negative in 149/152 controls, resulting in a sensitivity of 95.9% (95% CI: 90.69–98.66) and a specificity of 98.0% (95% CI: 94.34–99.59), respectively. The sensitivity of the LAMP assay was 95.0% (95% CI: 88.61–98.34) in HIV-negatives and 100% (95% CI: 85.18–100.0) in HIV-positives. Compared with microscopy, LAMP detected 82/87 (94.3%, 95% CI: 87.10–98.11) of the microscopy1 cases and was negative in 11/27 (40.7%, 95% CI: 22.39–61.20) of the microscopy2 cases. Compared with the rK39 serology, LAMP detected 113/120 (94.2%, 95% CI: 88.35–97.62) of the rK391 cases and was negative in 149/154 (96.8%, 95% CI: 92.59–98.94) of the rK392 cases. However, when compared with microscopy only, rK39 detected 83/87 (95.4%, 95% CI: 88.64–98.73) of the microscopy1 cases and negative in only 12/27 (44.4%, 95% CI: 25.48–64.67) of the microscopy– cases. There was an excellent agreement between rK39 and LAMP (Kappa 5 0.91, 95% CI: 0.86–0.96). Furthermore, an algorithm using rK39 followed by LAMP would yield a sensitivity of 99.2% (95%CI: 95.52–99.89) and a specificity of 98.0% (95% CI: 94.34–99.59). The findings demonstrate that the LAMP assay is an accurate and rapid molecular assay for VL diagnosis, including in HIV-1 co-infected patients, in an endemic setting. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=visceral%20leishmaniasis" title="visceral leishmaniasis">visceral leishmaniasis</a>, <a href="https://publications.waset.org/abstracts/search?q=HIV" title=" HIV"> HIV</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnosis" title=" diagnosis"> diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=LAMP" title=" LAMP"> LAMP</a>, <a href="https://publications.waset.org/abstracts/search?q=Ethiopia" title=" Ethiopia"> Ethiopia</a> </p> <a href="https://publications.waset.org/abstracts/162163/utility-of-the-loop-mediated-isothermal-amplification-assay-for-the-diagnosis-of-visceral-leishmaniasis-from-blood-samples-in-ethiopia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/162163.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">98</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">29</span> A Comprehensive Analysis of LACK (Leishmania Homologue of Receptors for Activated C Kinase) in the Context of Visceral Leishmaniasis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sukrat%20Sinha">Sukrat Sinha</a>, <a href="https://publications.waset.org/abstracts/search?q=Abhay%20Kumar"> Abhay Kumar</a>, <a href="https://publications.waset.org/abstracts/search?q=Shanthy%20Sundaram"> Shanthy Sundaram</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The Leishmania homologue of activated C kinase (LACK) is known T cell epitope from soluble Leishmania antigens (SLA) that confers protection against Leishmania challenge. This antigen has been found to be highly conserved among Leishmania strains. LACK has been shown to be protective against L. donovani challenge. A comprehensive analysis of several LACK sequences was completed. The analysis shows a high level of conservation, lower variability and higher antigenicity in specific portions of the LACK protein. This information provides insights for the potential consideration of LACK as a putative candidate in the context of visceral Leishmaniasis vaccine target. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bioinformatics" title="bioinformatics">bioinformatics</a>, <a href="https://publications.waset.org/abstracts/search?q=genome%20assembly" title=" genome assembly"> genome assembly</a>, <a href="https://publications.waset.org/abstracts/search?q=leishmania%20activated%20protein%20kinase%20c%20%28lack%29" title="leishmania activated protein kinase c (lack)">leishmania activated protein kinase c (lack)</a>, <a href="https://publications.waset.org/abstracts/search?q=next-generation%20sequencing" title=" next-generation sequencing"> next-generation sequencing</a> </p> <a href="https://publications.waset.org/abstracts/30204/a-comprehensive-analysis-of-lack-leishmania-homologue-of-receptors-for-activated-c-kinase-in-the-context-of-visceral-leishmaniasis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/30204.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">338</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28</span> Molecular Detection of Leishmania from the Phlebotomus Genus: Tendency towards Leishmaniasis Regression in Constantine, North-East of Algeria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=K.%20Frahtia">K. Frahtia</a>, <a href="https://publications.waset.org/abstracts/search?q=I.%20Mihoubi"> I. Mihoubi</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Picot"> S. Picot</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Leishmaniasis is a group of parasitic disease with a varied clinical expression caused by flagellate protozoa of the Leishmania genus. These diseases are transmitted to humans and animals by the sting of a vector insect, the female sandfly. Among the groups of dipteral disease vectors, Phlebotominae occupy a prime position and play a significant role in human pathology, such as leishmaniasis that affects nearly 350 million people worldwide. The vector control operation launched by health services throughout the country proves to be effective since despite the prevalence of the disease remains high especially in rural areas, leishmaniasis appears to be declining in Algeria. In this context, this study mainly concerns molecular detection of Leishmania from the vector. Furthermore, a molecular diagnosis has also been made on skin samples taken from patients in the region of Constantine, located in the North-East of Algeria. Concerning the vector, 5858 sandflies were captured, including 4360 males and 1498 females. Male specimens were identified based on their morphological. The morphological identification highlighted the presence of the Phlebotomus genus with a prevalence of 93% against 7% represented by the Sergentomyia genus. About the identified species, P. perniciosus is the most abundant with 59.4% of the male identified population followed by P. longicuspis with 24.7% of the workforce. P. perfiliewi is poorly represented by 6.7% of specimens followed by P. papatasi with 2.2% and 1.5% S. dreyfussi. Concerning skin samples, 45/79 (56.96%) collected samples were found positive by real-time PCR. This rate appears to be in sharp decline compared to previous years (alert peak of 30,227 cases in 2005). Concerning the detection of Leishmania from sandflies by RT-PCR, the results show that 3/60 PCR performed genus are positive with melting temperatures corresponding to that of the reference strain (84.1 +/- 0.4 ° C for L. infantum). This proves that the vectors were parasitized. On the other side, identification by RT-PCR species did not give any results. This could be explained by the presence of an insufficient amount of leishmanian DNA in the vector, and therefore support the hypothesis of the regression of leishmaniasis in Constantine. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Algeria" title="Algeria">Algeria</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20diagnostic" title=" molecular diagnostic"> molecular diagnostic</a>, <a href="https://publications.waset.org/abstracts/search?q=phlebotomus" title=" phlebotomus"> phlebotomus</a>, <a href="https://publications.waset.org/abstracts/search?q=real%20time%20PCR" title=" real time PCR"> real time PCR</a> </p> <a href="https://publications.waset.org/abstracts/44561/molecular-detection-of-leishmania-from-the-phlebotomus-genus-tendency-towards-leishmaniasis-regression-in-constantine-north-east-of-algeria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/44561.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">272</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27</span> Gossypol Extraction from Cotton Seed and Evaluation of Cotton Seed and Boll-cotton-pol Extract on Treatment of Cutaneous Leishmaniasis Resistant to Drugs</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20Mirmohammadi">M. Mirmohammadi</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Taghdisi"> S. Taghdisi</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Anali"> F. Anali</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Gossypol is a yellow anti-nutritional compound found in the cotton plant. This substance exists in the cottonseed and other parts of the cotton plant, such as bark, leaves, and stems. Chemically, gossypol is a very active polyphenolic aldehyde compound, and due to this polyphenolic structure, it has antioxidant and therapeutic properties. On the other hand, this compound, especially in free form, has many toxic effects, that its excessive consumption can be very dangerous for humans and animals. In this study, gossypol was extracted as a derivative compound of gossypol acetic acid from cottonseed using the n-hexane solvent with an efficiency of 0.84 ± 0.04, which compared to the Gossypol extracted from cottonseed oil with the same method (cold press) showed a significant difference with its efficiency of 1.14 ± 0.06. Therefore, it can be suggested to use cottonseed oil to extract this valuable compound. In the other part of this research, cottonseed extracts and cotton bolls extracts were obtained by two methods of soaking and Soxhlet with hydroalcoholic solvent taken with a ratio of (25:75), then by using extracts and corn starch powder, four herbal medicine code was created and after receiving the code of ethics (IR.SSU.REC.1398.136) the therapeutic effect of each one on the Cutaneous leishmaniasis resistant to drugs (caused by the leishmaniasis parasite) was investigated in real patients and its results was compared with the common drug glucantime (local ampoule) (n = 36). Statistical studies showed that the use of herbal medicines prepared with cottonseed extract and cotton bolls extract has a significant positive effect on the treatment of the disease’s wounds (p-value > 0.05) compared to the control group (only ethanol). Also, by comparing the average diameter of the wounds after a two-month treatment period, no significant difference was found between the use of ointment containing extracts and local glucantime ampoules (p-value < 0.05). Bolls extract extracted with the Soxhlet method showed the best therapeutic effects, although there was no significant difference between them (p-value < 0.05). Therefore, there is acceptable reliability to recommend this medicine for the treatment of Cutaneous leishmaniasis resistant to drugs without the side effects of the chemical drug glucantime and the pain of injecting the ampoule. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cottonseed%20oil" title="cottonseed oil">cottonseed oil</a>, <a href="https://publications.waset.org/abstracts/search?q=gossypol" title=" gossypol"> gossypol</a>, <a href="https://publications.waset.org/abstracts/search?q=cotton%20boll" title=" cotton boll"> cotton boll</a>, <a href="https://publications.waset.org/abstracts/search?q=cutaneous%20leishmaniasis" title=" cutaneous leishmaniasis"> cutaneous leishmaniasis</a> </p> <a href="https://publications.waset.org/abstracts/170552/gossypol-extraction-from-cotton-seed-and-evaluation-of-cotton-seed-and-boll-cotton-pol-extract-on-treatment-of-cutaneous-leishmaniasis-resistant-to-drugs" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/170552.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">95</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">26</span> Hematological Profiles of Visceral Leishmaniasis Patients before and after Treatment of Anti-Leishmanial Drugs at University of Gondar Leishmania Research and Treatment Center Northwest, Ethiopia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Fitsumbrhan%20Tajebe">Fitsumbrhan Tajebe</a>, <a href="https://publications.waset.org/abstracts/search?q=Fadil%20Murad"> Fadil Murad</a>, <a href="https://publications.waset.org/abstracts/search?q=Mitikie%20%20Tigabie"> Mitikie Tigabie</a>, <a href="https://publications.waset.org/abstracts/search?q=Mareye%20Abebaw"> Mareye Abebaw</a>, <a href="https://publications.waset.org/abstracts/search?q=Tadele%20Alemu"> Tadele Alemu</a>, <a href="https://publications.waset.org/abstracts/search?q=Sefanit%20Abate"> Sefanit Abate</a>, <a href="https://publications.waset.org/abstracts/search?q=Rezika%20Mohammedw"> Rezika Mohammedw</a>, <a href="https://publications.waset.org/abstracts/search?q=Arega%20Yeshanew"> Arega Yeshanew</a>, <a href="https://publications.waset.org/abstracts/search?q=Elias%20Shiferaw"> Elias Shiferaw</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Visceral leshimaniasis is a parasitic disease characterized by a systemic infection of phagocytic cells. Hematological parameters of these patients may be affected by the progress of the disease or treatment. Thus, the current study aimed to assess the hematological profiles of visceral leishmaniasis patients before and after treatment. Method: An institutional based retrospective cohort study was conducted among visceral leishmaniasis patients at University of Gondar Comprehensive Specialized Referral Hospital Leishmaniasis Research and Treatment Center from 2013 to 2018. Hematological profiles before initiation and after completion of treatment were extracted from registration book. Descriptive statics was presented using frequency and percentage. Paired t-test and Wilcoxon Signed rank test were used for comparing mean difference for normally and non- normally distributed data, respectively. Spearman and Pearson correlation analysis was used to describe the correlation of hematological parameters with different variables. P value < 0.05 was considered as statistically significant. Result: Except absolute nerutrophil count, post treatment hematological parameters show a significant increment compared to pretreatment one. The prevalence of anemia, leucopenia and thrombocytopenia was 85.5%, 83.4% and 75.8% prior to treatment and it was 58.3%, 38.2% and 19.2% after treatment, respectively. Moreover, parasite load of the disease showed statistically significant negative correlation with hematological profiles mainly with white blood cell and red blood cell. Conclusion: Majority of hematological profiles of patients with active VL have been restored after treatment, which might be associated with treatment effect on parasite proliferation and concentration of parasite in visceral organ, which directly affect hematological profiles. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=visceral%20leshimaniasis" title="visceral leshimaniasis">visceral leshimaniasis</a>, <a href="https://publications.waset.org/abstracts/search?q=hematological%20profile" title=" hematological profile"> hematological profile</a>, <a href="https://publications.waset.org/abstracts/search?q=anti-leshimanial%20drug" title=" anti-leshimanial drug"> anti-leshimanial drug</a>, <a href="https://publications.waset.org/abstracts/search?q=Gondar" title=" Gondar"> Gondar</a> </p> <a href="https://publications.waset.org/abstracts/122906/hematological-profiles-of-visceral-leishmaniasis-patients-before-and-after-treatment-of-anti-leishmanial-drugs-at-university-of-gondar-leishmania-research-and-treatment-center-northwest-ethiopia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/122906.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">128</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">25</span> Bayesian Locally Approach for Spatial Modeling of Visceral Leishmaniasis Infection in Northern and Central Tunisia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kais%20Ben-Ahmed">Kais Ben-Ahmed</a>, <a href="https://publications.waset.org/abstracts/search?q=Mhamed%20Ali-El-Aroui"> Mhamed Ali-El-Aroui</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This paper develops a Local Generalized Linear Spatial Model (LGLSM) to describe the spatial variation of Visceral Leishmaniasis (VL) infection risk in northern and central Tunisia. The response from each region is a number of affected children less than five years of age recorded from 1996 through 2006 from Tunisian pediatric departments and treated as a poison county level data. The model includes climatic factors, namely averages of annual rainfall, extreme values of low temperatures in winter and high temperatures in summer to characterize the climate of each region according to each continentality index, the pluviometric quotient of Emberger (Q2) to characterize bioclimatic regions and component for residual extra-poison variation. The statistical results show the progressive increase in the number of affected children in regions with high continentality index and low mean yearly rainfull. On the other hand, an increase in pluviometric quotient of Emberger contributed to a significant increase in VL incidence rate. When compared with the original GLSM, Bayesian locally modeling is improvement and gives a better approximation of the Tunisian VL risk estimation. According to the Bayesian approach inference, we use vague priors for all parameters model and Markov Chain Monte Carlo method. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=generalized%20linear%20spatial%20model" title="generalized linear spatial model">generalized linear spatial model</a>, <a href="https://publications.waset.org/abstracts/search?q=local%20model" title=" local model"> local model</a>, <a href="https://publications.waset.org/abstracts/search?q=extra-poisson%20variation" title=" extra-poisson variation"> extra-poisson variation</a>, <a href="https://publications.waset.org/abstracts/search?q=continentality%20index" title=" continentality index"> continentality index</a>, <a href="https://publications.waset.org/abstracts/search?q=visceral%20leishmaniasis" title=" visceral leishmaniasis"> visceral leishmaniasis</a>, <a href="https://publications.waset.org/abstracts/search?q=Tunisia" title=" Tunisia"> Tunisia</a> </p> <a href="https://publications.waset.org/abstracts/14074/bayesian-locally-approach-for-spatial-modeling-of-visceral-leishmaniasis-infection-in-northern-and-central-tunisia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/14074.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">397</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">24</span> Endeavor to Develop Immunological and Hematological Early Diagnostic Marker to Check the Conversion of Asymptomatic to Symptomatic Visceral Leishmaniasis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Roshan%20Kamal%20Topno">Roshan Kamal Topno</a>, <a href="https://publications.waset.org/abstracts/search?q=Maneesh%20Kumar"> Maneesh Kumar</a>, <a href="https://publications.waset.org/abstracts/search?q=Manas%20Ranjan%20Dikhit"> Manas Ranjan Dikhit</a>, <a href="https://publications.waset.org/abstracts/search?q=Krishna%20Pandey"> Krishna Pandey</a>, <a href="https://publications.waset.org/abstracts/search?q=Major%20Madhukar"> Major Madhukar</a>, <a href="https://publications.waset.org/abstracts/search?q=Vidhya%20Nand%20Rabidas"> Vidhya Nand Rabidas</a>, <a href="https://publications.waset.org/abstracts/search?q=Vahab%20Ali"> Vahab Ali</a>, <a href="https://publications.waset.org/abstracts/search?q=Ganesh%20Chandra%20Sahoo"> Ganesh Chandra Sahoo</a>, <a href="https://publications.waset.org/abstracts/search?q=Bhawana"> Bhawana</a>, <a href="https://publications.waset.org/abstracts/search?q=Devendra%20Prasad%20Yadav"> Devendra Prasad Yadav</a>, <a href="https://publications.waset.org/abstracts/search?q=Rishikesh%20Kumar"> Rishikesh Kumar</a>, <a href="https://publications.waset.org/abstracts/search?q=Pradeep%20Das"> Pradeep Das</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A diagnostic marker for asymptomatic subject becomes a crucial need for advocating early prophylactic majors to control protozoal infection. The main issue in epidemiological affected regions is the presence of an asymptomatic individual that might potentially convert to a symptomatic visceral leishmaniasis (VL). The epidemiological study has been conducted at highly VL endemic Moriyama village in Patna district, Bihar, India that covers total population of 1540 individuals. Here, 1104(74.02%) people had been randomly screened and only 46 (4.17%) asymptomatic individuals were found sero-positive by the rK39 test. After taking signed informed consent form, blood samples were collected from 46 asymptomatic subjects for further hematological and immunological tests. Total leukocyte count, hemoglobin (gm%), neutrophil, lymphocyte, platelet count and interleukin-10 (IL-10) had been included as diagnostic markers. Interestingly only 5 (10.86%) individuals showed their asymptomatic conversion into symptomatic VL patients during quarterly surveillance. In overall analysis only two markers are suggestive for disease conversion that is hemoglobin (gm%) and IL-10. In all the infected patients, both the mean decrease in hemoglobin and mean increase of IL-10 was 19.23% from its normal value. The results might suggest that hematological and immunological changes would become helpful for early diagnosis of asymptomatic to symptomatic VL conversion. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=asymptomatic" title="asymptomatic">asymptomatic</a>, <a href="https://publications.waset.org/abstracts/search?q=epidemiological" title=" epidemiological"> epidemiological</a>, <a href="https://publications.waset.org/abstracts/search?q=symptomatic%20visceral%20leishmaniasis" title=" symptomatic visceral leishmaniasis"> symptomatic visceral leishmaniasis</a>, <a href="https://publications.waset.org/abstracts/search?q=hemoglobin%20%28gm%25%29" title=" hemoglobin (gm%)"> hemoglobin (gm%)</a>, <a href="https://publications.waset.org/abstracts/search?q=interleukin-10" title=" interleukin-10"> interleukin-10</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnosis" title=" diagnosis"> diagnosis</a> </p> <a href="https://publications.waset.org/abstracts/99701/endeavor-to-develop-immunological-and-hematological-early-diagnostic-marker-to-check-the-conversion-of-asymptomatic-to-symptomatic-visceral-leishmaniasis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/99701.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">209</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">23</span> Investigation of Leishmaniasis, Babesiosis, Ehrlichiosis, Dirofilariasis, and Hepatozoonosis in Referred Dogs to Veterinary Hospitals in Tehran, 2022</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohamad%20Bolandmartabe">Mohamad Bolandmartabe</a>, <a href="https://publications.waset.org/abstracts/search?q=Nafiseh%20Hassani"> Nafiseh Hassani</a>, <a href="https://publications.waset.org/abstracts/search?q=Saeed%20Abdi%20Darake"> Saeed Abdi Darake</a>, <a href="https://publications.waset.org/abstracts/search?q=Maryam%20Asghari"> Maryam Asghari</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Dogs are highly susceptible to diseases, nutritional problems, toxins, and parasites, with parasitic infections being common and causing hardship in their lives. Some important internal parasites include worms (such as roundworms and tapeworms) and protozoa, which can lead to anemia in dogs. Important bloodborne parasites in dogs include microfilariae and adult forms of Dirofilaria immitis, Dipetalonema reconditum, Babesia, Trypanosoma, Hepatozoon, Leishmania, Ehrlichia, and Hemobartonella. Babesia and Hemobartonella are parasites that reside inside red blood cells and cause regenerative anemia by directly destroying the red blood cells. Hepatozoon, Leishmania, and Ehrlichia are also parasites that reside within white blood cells and can infiltrate other tissues, such as the liver and lymph nodes. Since intermediate hosts are more commonly found in the open environment, the prevalence of parasites in stray and free-roaming dogs is higher compared to pet dogs. Furthermore, pet dogs are less exposed to internal and external parasites due to better care, hygiene, and being predominantly indoors. Therefore, they are less likely to be affected by them. Among the parasites, Leishmania carries significant importance as it is shared between dogs and humans, causing a dangerous disease known as visceral Leishmaniasis or kala-azar and cutaneous Leishmaniasis. Furthermore, dogs can act as reservoirs and spread the disease agent within human communities. Therefore, timely and accurate diagnosis of these diseases in dogs can be highly beneficial in preventing their occurrence in humans. In this article, we employed the Giemsa staining technique under a light microscope for the identification of bloodborne parasites in dogs. However, considering the negative impact of these parasites on the natural life of dogs, the development of chronic diseases, and the gradual loss of the animal's well-being, rapid and timely diagnosis is essential. Serological methods and PCR are available for the diagnosis of certain parasites, which have high sensitivity and desirable characteristics. Therefore, this research aims to investigate the molecular aspects of bloodborne parasites in dogs referred to veterinary hospitals in Tehran city. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=leishmaniasis" title="leishmaniasis">leishmaniasis</a>, <a href="https://publications.waset.org/abstracts/search?q=babesiosis" title=" babesiosis"> babesiosis</a>, <a href="https://publications.waset.org/abstracts/search?q=ehrlichiosis" title=" ehrlichiosis"> ehrlichiosis</a>, <a href="https://publications.waset.org/abstracts/search?q=dirofilariasis" title=" dirofilariasis"> dirofilariasis</a>, <a href="https://publications.waset.org/abstracts/search?q=hepatozoonosis" title=" hepatozoonosis"> hepatozoonosis</a> </p> <a href="https://publications.waset.org/abstracts/169495/investigation-of-leishmaniasis-babesiosis-ehrlichiosis-dirofilariasis-and-hepatozoonosis-in-referred-dogs-to-veterinary-hospitals-in-tehran-2022" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/169495.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">101</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">22</span> Infection of Phlebotomus Sergenti with Leishmania Tropica in a Classical Focus of Leishmania Major in Tunisia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kaouther%20Jaouadi">Kaouther Jaouadi</a>, <a href="https://publications.waset.org/abstracts/search?q=Jihene%20Bettaieb"> Jihene Bettaieb</a>, <a href="https://publications.waset.org/abstracts/search?q=Amira%20Bennour"> Amira Bennour</a>, <a href="https://publications.waset.org/abstracts/search?q=Ghassen%20Kharroubi"> Ghassen Kharroubi</a>, <a href="https://publications.waset.org/abstracts/search?q=Sadok%20Salem"> Sadok Salem</a>, <a href="https://publications.waset.org/abstracts/search?q=Afif%20Ben%20Salah"> Afif Ben Salah</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In Tunisia, chronic cutaneous leishmaniasis due to Leishmania (L) tropica is an important health problem. Its spreading has not been fully elucidated. Information on sandfly vectors, as well as their associated Leishmania species, is of paramount importance since vector dispersion is one of the major factors responsible for pathogen dissemination. In total, 650 sandflies were captured between June and August 2015 using sticky paper traps in the governorate of Sidi Bouzid, a classical focus of L. major in the Central-West of Tunisia. Polymerase chain reaction-restriction fragment length polymorphism analysis of the internal transcribed spacer 1 and sequencing were used for Leishmania detection and identification. Ninety-seven unfed females were tested for the presence of Leishmania parasite DNA. Six Phlebotomus sergenti were found positive for L. tropica. This finding enhances the understanding of the cycle extension of L. tropica outside its original focus of Tataouine in the South-East of the country. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cutaneous%20leishmaniasis" title="cutaneous leishmaniasis">cutaneous leishmaniasis</a>, <a href="https://publications.waset.org/abstracts/search?q=Leishmania%20tropica" title=" Leishmania tropica"> Leishmania tropica</a>, <a href="https://publications.waset.org/abstracts/search?q=sandflies" title=" sandflies"> sandflies</a>, <a href="https://publications.waset.org/abstracts/search?q=Tunisia" title=" Tunisia"> Tunisia</a> </p> <a href="https://publications.waset.org/abstracts/127947/infection-of-phlebotomus-sergenti-with-leishmania-tropica-in-a-classical-focus-of-leishmania-major-in-tunisia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/127947.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">156</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">21</span> Ribosomal Protein S4 Gene: Exploring the Presence in Syrian Strain of Leishmania Tropica Genome, Sequencing it and Evaluating Immune Response of pCI-S4 DNA Vaccine</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alyaa%20Abdlwahab">Alyaa Abdlwahab</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cutaneous leishmaniasis represents a serious health problem in Syria; this problem has become noticeably aggravated after the civil war in the country. Leishmania tropica parasite is the main cause of cutaneous leishmaniasis in Syria. In order to control the disease, we need an effective vaccine against leishmania parasite. DNA vaccination remains one of the favorable approaches that have been used to face cutaneous leishmaniasis. Ribosomal protein S4 is responsible for important roles in Leishmania parasite life. DNA vaccine based on S4 gene has been used against infections by many species of Leishmania parasite but leishmania tropica parasite, so this gene represents a good candidate for DNA vaccine construction. After proving the existence of ribosomal protein S4 gene in a Syrian strain of Leishmania tropica (LCED Syrian 01), sequencing it and cloning it into pCI plasmid, BALB/C mice were inoculated with pCI-S4 DNA vaccine. The immune response was determined by monitoring the lesion progression in inoculated BALB/C mice for six weeks after challenging mice with Leishmania tropica (LCED Syrian 01) parasites. IL-12, IFN-γ, and IL-4 were quantified in draining lymph nodes (DLNa) of the immunized BALB/C mice by using the RT-qPCR technique. The parasite burden was calculated in the final week for the footpad lesion and the DLNs of the mice. This study proved the existence and the expression of the ribosomal protein S4 gene in Leishmania tropica (LCED Syrian 01) promastigotes. The sequence of ribosomal protein cDNA S4 gene was determined and published in Genbank; the gene size was 822 bp. Expression was also demonstrated at the level of cDNA. Also, this study revealed that pCI-S4 DNA vaccine induces TH1\TH2 response in immunized mice; this response prevents partially developing a dermal lesion of Leishmania. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ribosomal%20protein%20S4" title="ribosomal protein S4">ribosomal protein S4</a>, <a href="https://publications.waset.org/abstracts/search?q=DNA%20vaccine" title=" DNA vaccine"> DNA vaccine</a>, <a href="https://publications.waset.org/abstracts/search?q=Leishmania%20tropica" title=" Leishmania tropica"> Leishmania tropica</a>, <a href="https://publications.waset.org/abstracts/search?q=BALB%5Cc" title=" BALB\c"> BALB\c</a> </p> <a href="https://publications.waset.org/abstracts/146394/ribosomal-protein-s4-gene-exploring-the-presence-in-syrian-strain-of-leishmania-tropica-genome-sequencing-it-and-evaluating-immune-response-of-pci-s4-dna-vaccine" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/146394.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">136</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">20</span> The Survey of Phlebotomine Sandfly (Diptera: Psychodidae) of Al-Asaba Area in the Northwest Region of the Libya</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Asherf%20El-Abaied">Asherf El-Abaied</a>, <a href="https://publications.waset.org/abstracts/search?q=Elsadik%20Anan"> Elsadik Anan</a>, <a href="https://publications.waset.org/abstracts/search?q=Badereddin%20Annajar"> Badereddin Annajar</a>, <a href="https://publications.waset.org/abstracts/search?q=Mustafa%20Saieh"> Mustafa Saieh</a>, <a href="https://publications.waset.org/abstracts/search?q=Abudalnaser%20El-Buni"> Abudalnaser El-Buni</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Zoonotic Cutaneous Leishmaniasis (ZCL) has been endemic in the Northwestern region of Libya for over nine decades. Survey of sandfly fauna in the region revealed that 13 species have been recorded with various distribution and abundance patterns. Phlebotomus papatasi proved to be the main vector of the disease in many areas. To identify sandfly species present in the Al-Asaba town and determine their spatial and seasonal abundance. An epidemiological analysis of the data obtained from the recorded cases was also carried out. Sand flies collected from various sites using sticky traps and CDC miniature light traps during the period from March-November 2006. Recorded ZCL cases were collected from the local Primary Health Care Department and analysed using SPSS statistical package. Ten species of sandflies were identified, seven belong to the genus Phlebotomus and three belong to the genus Sergentomyia. P. papatasi was the most abundant species with peak season recorded in September. The prevalence of the disease was low however; notable increase of ZCL cases in last three years has been indicated. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Cutaneous%20leishmaniasis" title="Cutaneous leishmaniasis">Cutaneous leishmaniasis</a>, <a href="https://publications.waset.org/abstracts/search?q=Phlebotomus%20papatasi" title=" Phlebotomus papatasi"> Phlebotomus papatasi</a>, <a href="https://publications.waset.org/abstracts/search?q=sandfly%20fauna" title=" sandfly fauna"> sandfly fauna</a>, <a href="https://publications.waset.org/abstracts/search?q=Libya" title=" Libya"> Libya</a> </p> <a href="https://publications.waset.org/abstracts/5821/the-survey-of-phlebotomine-sandfly-diptera-psychodidae-of-al-asaba-area-in-the-northwest-region-of-the-libya" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/5821.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">302</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">19</span> Exploring the Role of Immune-Modulators in Pathogen Recognition Receptor NOD2 Mediated Protection against Visceral Leishmaniasis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Junaid%20Jibran%20Jawed">Junaid Jibran Jawed</a>, <a href="https://publications.waset.org/abstracts/search?q=Prasanta%20Saini"> Prasanta Saini</a>, <a href="https://publications.waset.org/abstracts/search?q=Subrata%20Majumdar"> Subrata Majumdar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Leishmania donovani infection causes severe host immune-suppression through the modulation of pathogen recognition receptors. Apart from TLRs (Toll Like Receptor), recent studies focus on the important contribution of NLR (NOD-Like Receptor) family member NOD1 and NOD2 as these receptors are capable of triggering host innate immunity. The aim of this study was to decipher the role of NOD1/NOD2 receptors during experimental visceral leishmaniasis (VL) and the important link between host failure and parasite evasion strategy. Method: The status of NOD1 and NOD2 receptors were analysed in uninfected and infected cells through western blotting and RT-PCR. The active contributions of these receptors in reducing parasite burden were confirmed by siRNA mediated silencing, and over-expression studies and the parasite numbers were calculated through microscopic examination of the Giemsa-stained slides. In-vivo studies were done by using non-toxic dose of Mw (Mycobacterium indicus pranii), Ara-LAM(Arabinoasylated lipoarabinomannan) along with MDP (Muramyl dipeptide) administration. Result: Leishmania donovani infection of the macrophages reduced the expression of NOD2 receptors whereas NOD1 remain unaffected. MDP, a NOD2-ligand, treatment during over-expression of NOD2, reduced the parasite burden effectively which was associated with increased pro-inflammatory cytokine generation and NO production. In experimental mouse model, Ara-LAM treatment increased the expression of NOD2 and in combination with MDP it showed active therapeutic potential against VL and found to be more effective than Mw which was already reported to be involved in NOD2 modulation. Conclusion: This work explores the essential contribution of NOD2 during experimental VL and mechanistic understanding of Ara-LAM + MDP combination therapy to work against this disease and highlighted NOD2 as an essential therapeutic target. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ara-LAM%20%28Arabinoacylated%20Lipoarabinomannan%29" title="Ara-LAM (Arabinoacylated Lipoarabinomannan)">Ara-LAM (Arabinoacylated Lipoarabinomannan)</a>, <a href="https://publications.waset.org/abstracts/search?q=NOD2%20%28nucleotide%20binding%20oligomerization%20receptor%202%29" title=" NOD2 (nucleotide binding oligomerization receptor 2)"> NOD2 (nucleotide binding oligomerization receptor 2)</a>, <a href="https://publications.waset.org/abstracts/search?q=MDP%20%28muramyl%20di%20peptide%29" title=" MDP (muramyl di peptide)"> MDP (muramyl di peptide)</a>, <a href="https://publications.waset.org/abstracts/search?q=visceral%20Leishmaniasis" title=" visceral Leishmaniasis"> visceral Leishmaniasis</a> </p> <a href="https://publications.waset.org/abstracts/80536/exploring-the-role-of-immune-modulators-in-pathogen-recognition-receptor-nod2-mediated-protection-against-visceral-leishmaniasis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/80536.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">175</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">18</span> Etiologies of Megaloblastic Anemia in a Pediatric Hospital</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Atitallah%20Sofien">Atitallah Sofien</a>, <a href="https://publications.waset.org/abstracts/search?q=Bouyahia%20Olfa"> Bouyahia Olfa</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohsen%20S."> Mohsen S.</a>, <a href="https://publications.waset.org/abstracts/search?q=Boussetta%20Khadija"> Boussetta Khadija</a>, <a href="https://publications.waset.org/abstracts/search?q=Khemiri%20Monia"> Khemiri Monia</a>, <a href="https://publications.waset.org/abstracts/search?q=Fitouri%20Zohra"> Fitouri Zohra</a>, <a href="https://publications.waset.org/abstracts/search?q=Boukthir%20Samir"> Boukthir Samir</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Megaloblastic anemia (MA) is rare in children. The diversity of its etiologies can lead to misdiagnosis and may, therefore, delay the treatment. The aim of this study was to describe the epidemiological and etiological characteristics of children followed for MA at the Tunis children's hospital. Methodology: This is a retrospective study over a period of 25 years of all cases of MA in children in the Children's Hospital of Tunis. The diagnosis of MA was confirmed by myelogram in all patients. Results: We collected 29 observations, with an incidence of 1.2 cases/year and a sex ratio of 1. Sixty percent of the children were aged between 3 months and 2 years. The consultation time was between 15 and 30 days in a third of the patients. The clinical examination showed hypotrophy in 13% of cases, hepatosplenomegaly in 6% of cases, neurological or neurosensory damage in 23% of cases, and cardiac damage in 10% of children. MA was associated with thrombocytopenia in 65% of cases and leukoneutropenia in 24% of cases. One in 5 children had pancytopenia. The etiologies were mainly thiamine deficiency, Immerslund disease (20%), nutritional deficiency (13%), and Biermer anemia (13%). One of the patients presented an MA revealing visceral leishmaniasis. The outcome under vitamin B12, the dose of which was adapted to each etiology, was favorable for all patients. Conclusion: MA is rare in children with multiple etiologies that are mainly dominated by hereditary conditions and nutritional deficiencies, mainly in vitamin B12. The association with visceral leishmaniasis seems to be a particularity in our country not reported in the literature. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=megaloblastic%20anemia" title="megaloblastic anemia">megaloblastic anemia</a>, <a href="https://publications.waset.org/abstracts/search?q=children" title=" children"> children</a>, <a href="https://publications.waset.org/abstracts/search?q=vitamin%20B12" title=" vitamin B12"> vitamin B12</a>, <a href="https://publications.waset.org/abstracts/search?q=anemia" title=" anemia"> anemia</a> </p> <a href="https://publications.waset.org/abstracts/175680/etiologies-of-megaloblastic-anemia-in-a-pediatric-hospital" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/175680.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">66</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">17</span> Targeting Peptide Based Therapeutics: Integrated Computational and Experimental Studies of Autophagic Regulation in Host-Parasite Interaction</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Vrushali%20Guhe">Vrushali Guhe</a>, <a href="https://publications.waset.org/abstracts/search?q=Shailza%20Singh"> Shailza Singh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cutaneous leishmaniasis is neglected tropical disease present worldwide caused by the protozoan parasite Leishmania major, the therapeutic armamentarium for leishmaniasis are showing several limitations as drugs are showing toxic effects with increasing resistance by a parasite. Thus identification of novel therapeutic targets is of paramount importance. Previous studies have shown that autophagy, a cellular process, can either facilitate infection or aid in the elimination of the parasite, depending on the specific parasite species and host background in leishmaniasis. In the present study, our objective was to target the essential autophagy protein ATG8, which plays a crucial role in the survival, infection dynamics, and differentiation of the Leishmania parasite. ATG8 in Leishmania major and its homologue, LC3, in Homo sapiens, act as autophagic markers. Present study manifested the crucial role of ATG8 protein as a potential target for combating Leishmania major infection. Through bioinformatics analysis, we identified non-conserved motifs within the ATG8 protein of Leishmania major, which are not present in LC3 of Homo sapiens. Against these two non-conserved motifs, we generated a peptide library of 60 peptides on the basis of physicochemical properties. These peptides underwent a filtering process based on various parameters, including feasibility of synthesis and purification, compatibility with Selective Reaction Monitoring (SRM)/Multiple reaction monitoring (MRM), hydrophobicity, hydropathy index, average molecular weight (Mw average), monoisotopic molecular weight (Mw monoisotopic), theoretical isoelectric point (pI), and half-life. Further filtering criterion shortlisted three peptides by using molecular docking and molecular dynamics simulations. The direct interaction between ATG8 and the shortlisted peptides was confirmed through Surface Plasmon Resonance (SPR) experiments. Notably, these peptides exhibited the remarkable ability to penetrate the parasite membrane and exert profound effects on Leishmania major. The treatment with these peptides significantly impacted parasite survival, leading to alterations in the cell cycle and morphology. Furthermore, the peptides were found to modulate autophagosome formation, particularly under starved conditions, suggesting their involvement in disrupting the regulation of autophagy within Leishmania major. In vitro, studies demonstrated that the selected peptides effectively reduced the parasite load within infected host cells. Encouragingly, these findings were corroborated by in vivo experiments, which showed a reduction in parasite burden upon peptide administration. Additionally, the peptides were observed to affect the levels of LC3II within host cells. In conclusion, our findings highlight the efficacy of these novel peptides in targeting Leishmania major’s ATG8 and disrupting parasite survival. These results provide valuable insights into the development of innovative therapeutic strategies against leishmaniasis via targeting autophagy protein ATG8 of Leishmania major. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ATG8" title="ATG8">ATG8</a>, <a href="https://publications.waset.org/abstracts/search?q=leishmaniasis" title=" leishmaniasis"> leishmaniasis</a>, <a href="https://publications.waset.org/abstracts/search?q=surface%20plasmon%20resonance" title=" surface plasmon resonance"> surface plasmon resonance</a>, <a href="https://publications.waset.org/abstracts/search?q=MD%20simulation" title=" MD simulation"> MD simulation</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20docking" title=" molecular docking"> molecular docking</a>, <a href="https://publications.waset.org/abstracts/search?q=peptide%20designing" title=" peptide designing"> peptide designing</a>, <a href="https://publications.waset.org/abstracts/search?q=therapeutics" title=" therapeutics"> therapeutics</a> </p> <a href="https://publications.waset.org/abstracts/169688/targeting-peptide-based-therapeutics-integrated-computational-and-experimental-studies-of-autophagic-regulation-in-host-parasite-interaction" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/169688.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">82</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">16</span> Green Synthesis of Nano Liposomes Containing Berberine Chlorideagainst Leishmania major</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ali%20Fattahi%20Bafghi">Ali Fattahi Bafghi</a>, <a href="https://publications.waset.org/abstracts/search?q=Abolghasem%20Siyadatpanah"> Abolghasem Siyadatpanah</a>, <a href="https://publications.waset.org/abstracts/search?q=Farzaneh%20Mirzaei"> Farzaneh Mirzaei</a>, <a href="https://publications.waset.org/abstracts/search?q=Fahimeh%20Pournasir"> Fahimeh Pournasir</a>, <a href="https://publications.waset.org/abstracts/search?q=Roghayeh%20Norouzi"> Roghayeh Norouzi</a>, <a href="https://publications.waset.org/abstracts/search?q=Maria%20De%20Lourdes%20Pereira"> Maria De Lourdes Pereira</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Leishmaniasis caused by Leishmania major is one of the main infectious diseases that affect populations in developing countries around the world. We assessed the effectiveness of berberine chloride nano-liposome (BcNLs) against L. major promastigotes in vitro. Nano-liposomal berberine chloride was prepared using the thin-film hydration method and characterized based on encapsulation efficiency, size, and zeta potential. Anti-Leishmania effect of different concentrations (0.05-60 µg/ml) of BcNLs as studied in L. major [MRHO/IR/75/ER] at 24, 48, and 72 h using the hemocytometer technique. Berberine chloride was successfully loaded into nano-liposomes with an encapsulation efficiency of 85.54%. The surface charge of nanoparticles is neutral, and the morphology of nano-liposomal berberine chloride is spherical without any agglomeration. Cell viability assay was performed on the HFF cell line to show the biocompatibility of liposome nanoparticles. IC50 of BcNPs at 24, 48, and 72 h against L. major were found to be 7.6, 5.96, and 3.19 µg/ml, respectively. BcNLs showed a significant anti-Leishmania effect and induced a better and more tangible effect on the survival of L. major promastigotes and could be suitable candidates for further investigation. The results showed that the BcNLs agent is effective against L. major promastigotes and may be a promising alternative to current treatments. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Leishmania%20major" title="Leishmania major">Leishmania major</a>, <a href="https://publications.waset.org/abstracts/search?q=berberine%20chloride" title=" berberine chloride"> berberine chloride</a>, <a href="https://publications.waset.org/abstracts/search?q=nano-liposomes" title=" nano-liposomes"> nano-liposomes</a>, <a href="https://publications.waset.org/abstracts/search?q=cutaneous%20leishmaniasis" title=" cutaneous leishmaniasis"> cutaneous leishmaniasis</a> </p> <a href="https://publications.waset.org/abstracts/136889/green-synthesis-of-nano-liposomes-containing-berberine-chlorideagainst-leishmania-major" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/136889.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">151</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">15</span> Anti-Leishmanial Compounds from the Seaweed Padina pavonica</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nahal%20Najafi">Nahal Najafi</a>, <a href="https://publications.waset.org/abstracts/search?q=Afsaneh%20Yegdaneh"> Afsaneh Yegdaneh</a>, <a href="https://publications.waset.org/abstracts/search?q=Sedigheh%20Saberi"> Sedigheh Saberi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Leishmaniasis poses a substantial global risk, affecting millions and resulting in thousands of cases each year in endemic regions. Challenges in current leishmaniasis treatments include drug resistance, high toxicity, and pancreatitis. Marine compounds, particularly brown algae, serve as a valuable source of inspiration for discovering treatments against Leishmania. Material and method: Padina pavonica was collected from the Persian Gulf. The seaweeds were dried and extracted with methanol: ethylacetate (1:1). The extract was partitioned to hexane (Hex), dicholoromethane (DCM), butanol, and water by Kupchan partitioning method. Hex partition was fractionated by silica gel column chromatography to 10 fractions (Fr. 1-10). Fr. 6 was further separated by the normal phase HPLC method to yield compounds 1-3. The structures of isolated compounds were elucidated by NMR, Mass, and other spectroscopic methods. Hex and DCM partitions, Fr. 6 and compounds 1-3, were tested for anti-leishmanicidal activity. RAW cell lines were cultured in enriched RPMI (10% FBS, 1% pen-strep) in a 37°C CO2 5% incubator, while promastigote cells were initially cultured in NNN culture and subsequently transferred to the aforementioned medium. Cytotoxicity was assessed using MTT tests, anti-promastigote activity was evaluated through Hemocytometer chamber promastigote counting, and the impact of amastigote damage was determined by counting amastigotes within 100 macrophages. Results: NMR and Mass identified isolated compounds as fucosterol and two sulfoquinovosyldiacylglycerols (SQDG). Among the samples tested, Fr.6 exhibited the highest cytotoxicity (CC50=60.24), while compound 2 showed the lowest cytotoxicity (CC50=21984). Compound 1 and dichloromethane fraction demonstrated the highest and lowest anti-promastigote activity (IC50=115.7, IC50=16.42, respectively), and compound 1 and hexane fraction exhibited the highest and lowest anti-amastigote activity (IC50=7.874, IC50=40.18, respectively). Conclusion: All six samples, including Hex and DCM partitions, Fr.6, and compounds 1-3, demonstrate a noteworthy correlation between rising concentration and time, with a statistically significant P-value of ≤0.05. Considering the higher selectivity index of compound 2 compared to others, it can be inferred that the presence of sulfur groups and unsaturated chains potentially contributes to these effects by impeding the DNA polymerase, which, of course, needs more research. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Padina" title="Padina">Padina</a>, <a href="https://publications.waset.org/abstracts/search?q=leishmania" title=" leishmania"> leishmania</a>, <a href="https://publications.waset.org/abstracts/search?q=sulfoquinovosyldiacylglycerol" title=" sulfoquinovosyldiacylglycerol"> sulfoquinovosyldiacylglycerol</a>, <a href="https://publications.waset.org/abstracts/search?q=cytotoxicity" title=" cytotoxicity"> cytotoxicity</a> </p> <a href="https://publications.waset.org/abstracts/186826/anti-leishmanial-compounds-from-the-seaweed-padina-pavonica" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/186826.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">20</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">14</span> Determining the Effective Substance of Cottonseed Extract on the Treatment of Leishmaniasis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mehrosadat%20Mirmohammadi">Mehrosadat Mirmohammadi</a>, <a href="https://publications.waset.org/abstracts/search?q=Sara%20Taghdisi"> Sara Taghdisi</a>, <a href="https://publications.waset.org/abstracts/search?q=Ali%20Padash"> Ali Padash</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20Hossein%20Pazandeh"> Mohammad Hossein Pazandeh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Gossypol, a yellowish anti-nutritional compound found in cotton plants, exists in various plant parts, including seeds, husks, leaves, and stems. Chemically, gossypol is a potent polyphenolic aldehyde with antioxidant and therapeutic properties. However, its free form can be toxic, posing risks to both humans and animals. Initially, we extracted gossypol from cotton seeds using n-hexane as a solvent (yield: 84.0 ± 4.0%). We also obtained cotton seed and cotton boll extracts via Soxhlet extraction (25:75 hydroalcoholic ratio). These extracts, combined with cornstarch, formed four herbal medicinal formulations. Ethical approval allowed us to investigate their effects on Leishmania-caused skin wounds, comparing them to glucantime (local ampoule). Herbal formulas outperformed the control group (ethanol only) in wound treatment (p-value 0.05). The average wound diameter after two months did not significantly differ between plant extract ointments and topical glucantime. Notably, cotton boll extract with 1% extra gossypol crystal showed the best therapeutic effect. We extracted gossypol from cotton seeds using n-hexane via Soxhlet extraction. Saponification, acidification, and recrystallization steps followed. FTIR, UV-Vis, and HPLC analyses confirmed the product’s identity. Herbal medicines from cotton seeds effectively treated chronic wounds compared to the ethanol-only control group. Wound diameter differed significantly between extract ointments and glucantime injections. It seems that due to the presence of large amounts of fat in the oil, the extraction of gossypol from it faces many obstacles. The extraction of this compound with our technique showed that extraction from oil has a higher efficiency, perhaps because of the preparation of oil by cold pressing method, the possibility of losing this compound is much less than when extraction is done with Soxhlet. On the other hand, the gossypol in the oil is mostly bound to the protein, which somehow protects the gossypol until the last stage of the extraction process. Since this compound is very sensitive to light and heat, it was extracted as a derivative with acetic acid. Also, in the treatment section, it was found that the ointment prepared with the extract is more effective and Gossypol is one of the effective ingredients in the treatment. Therefore, gossypol can be extracted from the oil and added to the extract from which gossypol has been extracted to make an effective medicine with a certain dose. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cottonseed" title="cottonseed">cottonseed</a>, <a href="https://publications.waset.org/abstracts/search?q=glucantime" title=" glucantime"> glucantime</a>, <a href="https://publications.waset.org/abstracts/search?q=gossypol" title=" gossypol"> gossypol</a>, <a href="https://publications.waset.org/abstracts/search?q=leishmaniasis" title=" leishmaniasis"> leishmaniasis</a> </p> <a href="https://publications.waset.org/abstracts/184864/determining-the-effective-substance-of-cottonseed-extract-on-the-treatment-of-leishmaniasis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/184864.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">61</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">13</span> Pyrazolylpyrazolines: Design, Synthesis and Biological Evaluation as Dual Acting Antimalarial-Antileishmanial Agents</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Adnan%20Bekhit">Adnan Bekhit</a>, <a href="https://publications.waset.org/abstracts/search?q=Eskedar%20Lodebo"> Eskedar Lodebo</a>, <a href="https://publications.waset.org/abstracts/search?q=Ariaya%20Hymete"> Ariaya Hymete</a>, <a href="https://publications.waset.org/abstracts/search?q=Hanan%20Ragab"> Hanan Ragab</a>, <a href="https://publications.waset.org/abstracts/search?q=Alaa%20El-Din%20Bekhit"> Alaa El-Din Bekhit</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Malaria and leishmaniasis have emerged as serious universal health problems throughout history of mankind. According to the WHO 2008 malarial report, half of the world population is at risk of malarial infection with an estimate of 1 million deaths occurring annually mainly in the African region. Furthermore, 12-15 million people are infected with Leishmaniasis worldwide. Despite the continuous introduction of a large number of agents for the treatment of malaria, there is still unmet medical needs due to the emergence of resistance. Resistance has occurred for almost all therapeutic agents approved for the treatment of malaria. Accordingly, it was the aim of this work to design and synthesis a group of antimalarial-antileshmanial agents that would show inhibitory activity against chloroquine-resistant strain of Plasmodium falciparum. The synthesized compounds were designed to contain a pyrazolylpyrazoline moiety having an aromatic group (p-tolyl or p-chlorophenyl) at N1-position of one pyrazoline ring due to the reports of promising activities of such compounds. A formyl or acyl substituent was introduced at the N1-position of the other pyrazoline ring, to investigate the effect of bulkiness of acyl substituents at this position. The synthesized compounds were evaluated for their in-vivo antimalarial activity against Plasmodium berghei infected mice at dose levels of 20 and 30 mg/Kg. the two most active compounds were evaluated for their antimalarial activity against chloroquin-resistant strain (RKL9) of Plasmodium falciparum. In addition, the synthesized compounds were tested for their in-vitro antileshmanial activity against Leishmania aethiopica promastigotes and amastigotes. For both antimalarial and antileishmanial activities, compounds having an N1-p-tolyl group at the first pyrazoline ring did not require bulkiness at the second pyrazoline ring nitrogen where the compound bearing an acetyl group proved to be the most active of the whole series. On the other hand, bulkiness at the N1-position of the second pyazoline ring was necessary in case of compounds carrying the p-chlorophenyl group, where the two derivatives having an N1-butanoyl and an N1-benzoyl moieties at the second pyrazoline showed the best activity. Furthermore, the toxicity of the active compounds were tested and were proved to be non-toxic at 125, 250 and 500 mg/Kg. In addition, docking of the most active compound (having a p-tolyl group at the first pyrazoline-N and an acetyl moiety on the other pyrazoline-N) was performed against dihydrofolate reductase enzyme. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=pyrazoline%20derivatives" title="pyrazoline derivatives">pyrazoline derivatives</a>, <a href="https://publications.waset.org/abstracts/search?q=in-vivo%20antimalarial%20activity" title=" in-vivo antimalarial activity"> in-vivo antimalarial activity</a>, <a href="https://publications.waset.org/abstracts/search?q=docking" title=" docking"> docking</a>, <a href="https://publications.waset.org/abstracts/search?q=dihydrofolate%20reductase" title=" dihydrofolate reductase"> dihydrofolate reductase</a> </p> <a href="https://publications.waset.org/abstracts/62770/pyrazolylpyrazolines-design-synthesis-and-biological-evaluation-as-dual-acting-antimalarial-antileishmanial-agents" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/62770.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">341</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">12</span> Maximum Entropy Based Image Segmentation of Human Skin Lesion</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sheema%20Shuja%20Khattak">Sheema Shuja Khattak</a>, <a href="https://publications.waset.org/abstracts/search?q=Gule%20Saman"> Gule Saman</a>, <a href="https://publications.waset.org/abstracts/search?q=Imran%20Khan"> Imran Khan</a>, <a href="https://publications.waset.org/abstracts/search?q=Abdus%20Salam"> Abdus Salam</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Image segmentation plays an important role in medical imaging applications. Therefore, accurate methods are needed for the successful segmentation of medical images for diagnosis and detection of various diseases. In this paper, we have used maximum entropy to achieve image segmentation. Maximum entropy has been calculated using Shannon, Renyi, and Tsallis entropies. This work has novelty based on the detection of skin lesion caused by the bite of a parasite called Sand Fly causing the disease is called Cutaneous Leishmaniasis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=shannon" title="shannon">shannon</a>, <a href="https://publications.waset.org/abstracts/search?q=maximum%20entropy" title=" maximum entropy"> maximum entropy</a>, <a href="https://publications.waset.org/abstracts/search?q=Renyi" title=" Renyi"> Renyi</a>, <a href="https://publications.waset.org/abstracts/search?q=Tsallis%20entropy" title=" Tsallis entropy"> Tsallis entropy</a> </p> <a href="https://publications.waset.org/abstracts/19990/maximum-entropy-based-image-segmentation-of-human-skin-lesion" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/19990.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">463</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=leishmaniasis&page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=leishmaniasis&page=2" rel="next">&rsaquo;</a></li> </ul> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" 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