The Technique for Excessive-Results of Recombinant Protein P

<!DOCTYPE html> <html> <head> <meta charset="utf-8"> <meta name="viewport" content="width=device-width, initial-scale=1"> <title>The Technique for Excessive-Results of Recombinant Protein P | 122751</title> <meta name="keywords" content="Md Asaduzzaman1*, Lutfun Nahar2, Md. Bakhtiar Lijon3, Shahin Imran4, Mohammad Saidur Rhaman, Glutathione S-transferase (GST)-tag; Glutathione (GHS); pGEX/pET vectors; Recombinant protein expression; Protein purification"/> <meta name="description" content="Affinity Tags have been performed as the potential tools in the basic biological research field especially for production of recombinant protein and func..122751"/> <meta name="citation_publisher" content="Walsh Medical Media" /> <meta name="citation_journal_title" content="Journal of Microbial & Biochemical Technology"> <meta name="citation_title" content="The Technique for Excessive-Results of Recombinant Protein Purification led to GST-tag Affinity Chromatography: A Review"> <meta name="citation_author" content="Md Asaduzzaman1" /> <meta name="citation_author" content="Lutfun Nahar2" /> <meta name="citation_author" content="Md. Bakhtiar Lijon3" /> <meta name="citation_author" content="Shahin Imran4" /> <meta name="citation_author" content="Mohammad Saidur Rhaman" /> <meta name="citation_year" content="2023"> <meta name="citation_volume" content="15"> <meta name="citation_issue" content="6"> <meta name="citation_doi" content="10.35248/1948-5948.23.15.584"> <meta name="citation_issn" content="1948-5948"> <meta name="citation_publication_date" content="2023/12/28" /> <meta name="citation_firstpage" content="1"> <meta name="citation_lastpage" content="15"> <meta name="citation_abstract" content="Affinity Tags have been performed as the potential tools in the basic biological research field especially for production of recombinant protein and functional proteomics. Those affinity tags were wildly applied to simplify the purification of recombinant protein as well as differentiation of protein complex. Glutathione-S-Transferase (GST) tag has been extensively used in affinity chromatography for purification of fusion/recombinant protein to analysis of structure and function of protein, protein-protein interaction and to produce pharmaceutical product. In this review we describe the advantage of GST-tag in affinity chromatography technique as a method for inducible, high level protein expression and purification of recombinant protein. Recombinant protein which is expressed in a pGEX or pET vectors and that protein with GST-tag encoded at the NH2 or COOH-region of genome sequence. There are some expression vectors which has different site to approve for unidirectional insertion of the coding region DNA, promoter, primers, antibiotic, Ori and GST-tag into pGEX vectors. By used reduced Glutathione (GSH) during affinity chromatography the GST-tag with recombinant protein is eluted and stored it. Displacement of the GST-tag from the recombinant protein performed by protease enzyme for digestion which is purified by the application of another affinity technique."> <meta name="citation_fulltext_html_url" content="https://www.walshmedicalmedia.com/open-access/the-technique-for-excessiveresults-of-recombinant-protein-purification-led-to-gsttag-affinity-chromatography-a-review-122751.html"> <meta name="citation_pdf_url" content="https://www.walshmedicalmedia.com/open-access/the-technique-for-excessiveresults-of-recombinant-protein-purification-led-to-gsttag-affinity-chromatography-a-review.pdf"> <meta name="citation_abstract_html_url" content="https://www.walshmedicalmedia.com/abstract/the-technique-for-excessiveresults-of-recombinant-protein-purification-led-to-gsttag-affinity-chromatography-a-review-122751.html"> <meta itemprop="name" content="Walsh Medical Media" /> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <meta name="google-site-verification" 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Bakhtiar Lijon'>Md. Bakhtiar Lijon</a>, <a href='/author/shahin-imran-54637' class='latest_link_author' title='Shahin Imran'>Shahin Imran</a> and <a href='/author/mohammad-saidur-rhaman-54638' class='latest_link_author' title='Mohammad Saidur Rhaman'>Mohammad Saidur Rhaman</a> </p> <p><p>Affinity Tags have been performed as the potential tools in the basic biological research field especially for production of recombinant protein and functional proteomics. Those affinity tags were wildly applied to simplify the purification of recombinant protein as well as differentiation of protein complex. Glutathione-S-Transferase (GST) tag has been extensively used in affinity chromatography for purification of fusion/recombinant protein to analysis of structure and function of protein, protein-protein interaction and to produce pharmaceutical product. In this review we describe the advantage of GST-tag in affinity chromatography technique as a method for inducible, high level protein expression and purification of recombinant protein. Recombinant protein which is expressed in a pGEX or pET vectors and that protein with GST-tag encoded at the NH<sub>2</sub> or COOH-region of genome sequence. There are some expression vectors which has different site to approve for unidirectional insertion of the coding region DNA, promoter, primers, antibiotic, Ori and GST-tag into pGEX vectors. By used reduced Glutathione (GSH) during affinity chromatography the GST-tag with recombinant protein is eluted and stored it. Displacement of the GST-tag from the recombinant protein performed by protease enzyme for digestion which is purified by the application of another affinity technique.</p></p> <p class="mb-1 font-size-3 text-muted"> <b>Published Date: </b> 2023-12-28; <b>Received Date: </b>2023-11-27</p> <nav class="nav a-pl-0"> <a href="https://www.walshmedicalmedia.com/open-access/the-technique-for-excessiveresults-of-recombinant-protein-purification-led-to-gsttag-affinity-chromatography-a-review-122751.html" title="Click here" class="nav-link text-danger"><i class="fas fa-file-code"></i> HTML</a> <a href="https://www.walshmedicalmedia.com/open-access/the-technique-for-excessiveresults-of-recombinant-protein-purification-led-to-gsttag-affinity-chromatography-a-review.pdf" title="Click here" class="nav-link text-success"><i class="fas fa-file-pdf"></i> PDF</a> </nav> <div class="nav social-icons align-items-center"> <p class="m-0">Share this article:</p> <a class="nav-link" title="Click here" target="_blank" rel="noopener" href="https://www.facebook.com/sharer.php?u=https://www.walshmedicalmedia.com/abstract/the-technique-for-excessiveresults-of-recombinant-protein-purification-led-to-gsttag-affinity-chromatography-a-review-122751.html"><i class="fab fa-facebook-f"></i></a> <a class="nav-link" title="Click here" target="_blank" rel="noopener" href="https://twitter.com/share?url=https://www.walshmedicalmedia.com/abstract/the-technique-for-excessiveresults-of-recombinant-protein-purification-led-to-gsttag-affinity-chromatography-a-review-122751.html"><i class="fab fa-twitter"></i></a> <a class="nav-link" title="Click here" target="_blank" rel="noopener" href="https://www.linkedin.com/shareArticle?mini=true&url=https://www.walshmedicalmedia.com/abstract/the-technique-for-excessiveresults-of-recombinant-protein-purification-led-to-gsttag-affinity-chromatography-a-review-122751.html"><i class="fab fa-linkedin-in"></i></a> <a class="nav-link" title="Click here" target="_blank" rel="noopener" href="https://plus.google.com/share?url=https://www.walshmedicalmedia.com/abstract/the-technique-for-excessiveresults-of-recombinant-protein-purification-led-to-gsttag-affinity-chromatography-a-review-122751.html"><i class="fab fa-google-plus-g"></i></a> </div> </div> </div> </div> </section>   <div class="whatup"> <ul> <li><a href="tel:+44 1687550021">+44 1687550021 <i class="fab fa-whatsapp" aria-hidden="true"></i></a></li> </ul> </div> <section> <footer> <div class="custom-footer"> <div class="container"> <div class="row"> <div class="col-md-3 md-margin-bottom-40"> <a download="" href="/"><img id="logo-footer" src="/assets/images/footer-logo.png" alt="Walsh Medical Media" width="220px;"></a> <p class="py-3">Walsh Medical Media is a global leader in open access publishing, dedicated to advancing the frontiers of science and technology. 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