Search results for: Hyukjin Lee

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Lee</title> <meta name="description" content="Search results for: Hyukjin Lee"> <meta name="keywords" content="Hyukjin Lee"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="Hyukjin Lee"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 2</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: Hyukjin Lee</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> A Microfluidic Biosensor for Detection of EGFR 19 Deletion Mutation Targeting Non-Small Cell Lung Cancer on Rolling Circle Amplification</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ji%20Su%20Kim">Ji Su Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Bo%20Ram%20Choi"> Bo Ram Choi</a>, <a href="https://publications.waset.org/abstracts/search?q=Ju%20Yeon%20Cho"> Ju Yeon Cho</a>, <a href="https://publications.waset.org/abstracts/search?q=Hyukjin%20Lee"> Hyukjin Lee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Epidermal growth factor receptor (EGFR) 19 deletion mutation gene is over-expressed in carcinoma patient. EGFR 19 deletion mutation is known as typical biomarker of non-small cell lung cancer (NSCLC), which one section in the coding exon 19 of EGFR is deleted. Therefore, there have been many attempts over the years to detect EGFR 19 deletion mutation for replacing conventional diagnostic method such as PCR and tissue biopsy. We developed a simple and facile detection platform based on Rolling Circle Amplification (RCA), which provides highly amplified products in isothermal amplification of the ligated DNA template. Limit of detection (~50 nM) and a faster detection time (~30 min) could be achieved by introducing RCA. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=EGFR19" title="EGFR19">EGFR19</a>, <a href="https://publications.waset.org/abstracts/search?q=cancer" title=" cancer"> cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnosis" title=" diagnosis"> diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=rolling%20circle%20amplification%20%28RCA%29" title=" rolling circle amplification (RCA)"> rolling circle amplification (RCA)</a>, <a href="https://publications.waset.org/abstracts/search?q=hydrogel" title=" hydrogel"> hydrogel</a> </p> <a href="https://publications.waset.org/abstracts/72641/a-microfluidic-biosensor-for-detection-of-egfr-19-deletion-mutation-targeting-non-small-cell-lung-cancer-on-rolling-circle-amplification" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/72641.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">255</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Visual Detection of Escherichia coli (E. coli) through Formation of Beads Aggregation in Capillary Tube by Rolling Circle Amplification</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bo%20Ram%20Choi">Bo Ram Choi</a>, <a href="https://publications.waset.org/abstracts/search?q=Ji%20Su%20Kim"> Ji Su Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Juyeon%20Cho"> Juyeon Cho</a>, <a href="https://publications.waset.org/abstracts/search?q=Hyukjin%20Lee"> Hyukjin Lee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Food contaminated by bacteria (E.coli), causes food poisoning, which occurs to many patients worldwide annually. We have introduced an application of rolling circle amplification (RCA) as a versatile biosensor and developed a diagnostic platform composed of capillary tube and microbeads for rapid and easy detection of Escherichia coli (E. coli). When specific mRNA of E.coli is extracted from cell lysis, rolling circle amplification (RCA) of DNA template can be achieved and can be visualized by beads aggregation in capillary tube. In contrast, if there is no bacterial pathogen in sample, no beads aggregation can be seen. This assay is possible to detect visually target gene without specific equipment. It is likely to the development of a genetic kit for point of care testing (POCT) that can detect target gene using microbeads. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=rolling%20circle%20amplification%20%28RCA%29" title="rolling circle amplification (RCA)">rolling circle amplification (RCA)</a>, <a href="https://publications.waset.org/abstracts/search?q=Escherichia%20coli%20%28E.%20coli%29" title=" Escherichia coli (E. coli)"> Escherichia coli (E. coli)</a>, <a href="https://publications.waset.org/abstracts/search?q=point%20of%20care%20testing%20%28POCT%29" title=" point of care testing (POCT)"> point of care testing (POCT)</a>, <a href="https://publications.waset.org/abstracts/search?q=beads%20aggregation" title=" beads aggregation"> beads aggregation</a>, <a href="https://publications.waset.org/abstracts/search?q=capillary%20tube" title=" capillary tube"> capillary tube</a> </p> <a href="https://publications.waset.org/abstracts/72639/visual-detection-of-escherichia-coli-e-coli-through-formation-of-beads-aggregation-in-capillary-tube-by-rolling-circle-amplification" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/72639.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">364</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th 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Search results for: Hyukjin Lee