Human BMI-1 Antibody MAB33341: R&D Systems

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class="ds_title">Human BMI-1 Antibody </h1> <div class="dsSprite_rnd ds_brand_logo ds_rnd_logo"></div> </div> <div class="product_meta"> <span class="category_number">Catalog #: MAB33341</span> <br/> <span class="citations"> <a href="#product-citations" id="citation_count" class="jump_to" data-tab="3" data-tabname="product-citations"> 1 Citation </a> </span> <span class="reviews review_stars_5"> <a href="#product-reviews" id="product_reviews" class="jump_to" data-tab="6" data-tabname="product-reviews"> 1 Review </a> </span> <a href="/reviews/MAB33341" rel="nofollow"><span class="submit_review"><button class="btn btn-quaternary">Submit a Review</button></span></a> <span class="datasheet_link"> <a href="#product-datasheets">Datasheet / COA / SDS</a> </span> </div>  <div class="product_wrapper row"> <div class="product_ordering_wrapper col-xs-12 col-md-8 col-md-push-4"> <div class="product_ordering"> <form action="/products/human-bmi-1-antibody-384515_mab33341" method="post" id="atc-datasheet-form" accept-charset="UTF-8"><div><div id="atc_datasheet_block">     <table class="add_to_cart"> <thead> <tr> <th class="ds_catalog">Catalog #</th> <th class="ds_availability_column mobile-hide">Availability</th> <th class="ds_size_price_column">Size / Price</th> <th class="ds_quantity_column">Qty</th> </tr> </thead> <tbody> <tr class="et_atc_product" data-sku="MAB33341"> <td class="dat_catalog_column">MAB33341</td> <td class="et_atc_results" colspan="2"></td> <td class="ds_quantity ds_quantity_column"><div class="form-item form-item-catnum-mab33341 form-type-textfield form-group"><input class="atc_quantity atc_hidden atc_qty form-control form-text" placeholder="0" style="display:none;" type="text" id="edit-catnum-mab33341" name="catnum-MAB33341" value="" size="5" maxlength="3" /></div></td> </tr> <tr class="et_atc_product" data-sku="MAB33341-SP"> <td class="dat_catalog_column">MAB33341-SP</td> <td class="et_atc_results" colspan="2"></td> <td class="ds_quantity 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No. MAB33341 - Human BMI-1 Antibody" href="https://resources.rndsystems.com/images/datasheets/antibody/BMI-1_MAB33341_Western_Blot_6094.jpg"> <img fetchpriority="high" src="https://resources.rndsystems.com/images/datasheets/antibody/BMI-1_MAB33341_Western_Blot_6094.jpg" class="img-fluid" alt="Detection of Human BMI‑1 by Western Blot." title="Detection of Human BMI‑1 by Western Blot."> </a> <div class="search_image_other_count"> <span class="search_more_images">3 Images</span> </div> </div> </div> <div id="extra_images overview-images" class="hidden"> <a class="ds-image" data-toggle="lightbox" data-gallery="overview-images" data-footer="Detection of BMI‑1-regulated Genes by Chromatin Immunoprecipitation." data-title="Detection of BMI‑1-regulated Genes by Chromatin Immunoprecipitation." title="Cat. No. MAB33341 - Human BMI-1 Antibody" onClick="event.preventDefault();" href="https://resources.rndsystems.com/images/datasheets/antibody/BMI-1_MAB33341_Chromatin_Immunoprecipitation_11249.jpg"> <img src="https://resources.rndsystems.com/images/datasheets/antibody/BMI-1_MAB33341_Chromatin_Immunoprecipitation_11249.jpg" class="img-fluid" loading="lazy" alt="Detection of BMI‑1-regulated Genes by Chromatin Immunoprecipitation." title="Detection of BMI‑1-regulated Genes by Chromatin Immunoprecipitation."> </a> <a class="ds-image" data-toggle="lightbox" data-gallery="overview-images" data-footer="Detection of BMI‑1 in HeLa Human Cell Line by Flow Cytometry." data-title="Detection of BMI‑1 in HeLa Human Cell Line by Flow Cytometry." title="Cat. No. MAB33341 - Human BMI-1 Antibody" onClick="event.preventDefault();" href="https://resources.rndsystems.com/images/datasheets/antibody/BMI-1_MAB33341_Flow_Cytometry_8402.jpg"> <img src="https://resources.rndsystems.com/images/datasheets/antibody/BMI-1_MAB33341_Flow_Cytometry_8402.jpg" class="img-fluid" loading="lazy" alt="Detection of BMI‑1 in HeLa Human Cell Line by Flow Cytometry." title="Detection of BMI‑1 in HeLa Human Cell Line by Flow Cytometry."> </a> </div> </div> <div class="product_info_wrapper"> </div> <div id="view-more-target" class="datasheet_section_element"> <a href="/target/bmi-1?category=Primary Antibodies" class="btn btn-secondary">All BMI-1 Antibodies</a> </div> </div> <div class="tabbed_content" id="rnd_antibody"> <div id="ds_tabs" class="tab_menu"> <div class="tab tab_1 tab_open" data-tab="1" data-tabname="product-details" id="dsTabs_product-details"> <span>Product Details</span> </div> <div class="tab tab_3" data-tab="3" data-tabname="product-citations" id="dsTabs_product-citations"> <span>Citations (1)</span> </div> <div class="tab tab_4" data-tab="4" data-tabname="faqs" id="dsTabs_faqs"> <span>FAQs</span> </div> <div class="tab tab_5" data-tab="5" data-tabname="supplemental-products" id="dsTabs_supplemental-products"> <span>Supplemental Products</span> </div> <div class="tab tab_6" data-tab="6" data-tabname="product-reviews" id="dsTabs_product-reviews"> <span> Reviews (1) </span> </div> </div> <div class="tab_content tab_1 tab_open" data-tab="1" data-tabname="product-details" id="product-details"> <div class="datasheet_section_element"> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_summary" id="product-anchor-ds_summary">Summary</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_data_examples" id="product-anchor-ds_data_examples">Data Examples</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_preparation_storage" id="product-anchor-ds_preparation_storage">Preparation and Storage</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_calculator" id="product-anchor-ds_calculator">Reconstitution Calculator</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_background" id="product-anchor-ds_background">Background</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_datasheets" id="product-anchor-ds_datasheets">Product Datasheets</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_related_research_areas" id="product-anchor-ds_related_research_areas">Related Research Areas</a> </div> <h2 id="ds_summary">Human BMI-1 Antibody Summary</h2> <div class="datasheet_section"> <div class="ds_table summary_table"> <div class="ds_table_body">  <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Species Reactivity</div> <div class="ds_table_cell summary_table_data"> Human </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Specificity</div> <div class="ds_table_cell summary_table_data"> Detects human BMI-1 in direct ELISAs and Western blots. </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Source</div> <div class="ds_table_cell summary_table_data"> Monoclonal Mouse IgG<sub>2A</sub> Clone # 384515 </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Purification</div> <div class="ds_table_cell summary_table_data"> Protein A or G purified from hybridoma culture supernatant </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Immunogen</div> <div class="ds_table_cell summary_table_data"> <EM>E. coli</EM>-derived recombinant human BMI-1<br />Asp96-Gly326<br />Accession # P35226 </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Formulation</div> <div class="ds_table_cell summary_table_data"> Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. </div> </div> </div> </div> </div> <div id="customer_also_purchased_block" class="loader" data-catalogId="MAB33341"></div> <div id="ds_applications" class="datasheet_section"> <h2>Applications</h2> <div class="ds_table app_table"> <div class="ds_table_head"> <div class="ds_table_head_row"> <div class="ds_table_head_cell app_table_blank"></div> <div class="ds_table_head_cell app_table_rec_conc">Recommended Concentration</div> <div class="ds_table_head_cell app_table_sample">Sample</div> </div> </div> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell app_table_name">Western Blot</div> <div class="ds_table_cell app_table_dilution">0.1 µg/mL</div> <div class="ds_table_cell app_table_sample">See below</div> </div> <div class="ds_table_row"> <div class="ds_table_cell app_table_name">Chromatin Immunoprecipitation (ChIP)</div> <div class="ds_table_cell app_table_dilution">5 µg/5 x 10<sup>6</sup> cells</div> <div class="ds_table_cell app_table_sample">See below</div> </div> <div class="ds_table_row"> <div class="ds_table_cell app_table_name2">CyTOF-ready</div> <div class="ds_table_cell app_table_freeform">Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.</div> <div class="ds_table_cell app_table_sample"> </div> </div> <div class="ds_table_row"> <div class="ds_table_cell app_table_name">Intracellular Staining by Flow Cytometry</div> <div class="ds_table_cell app_table_dilution">2.5 µg/10<sup>6</sup> cells</div> <div class="ds_table_cell app_table_sample">See below</div> </div> </div> </div> <p>Please Note: Optimal dilutions should be determined by each laboratory for each application. <a href="/resources/protocols-troubleshooting-guides">General Protocols</a> are available in the Technical Information section on our website. </p> </div> <div id="ds_scientific_data" class="datasheet_section"> <div id="ds_data_examples"> <h2>Scientific Data</h2> <div id="data_examples"> <div class="row data_ex_row"> <a id="ds_image_0" class="pageAnchor"></a> <div class="data_ex_image_wrapper col-xs-12 col-sm-5 col-md-4"> <div class="data_ex_image"> <span class="data_ex_image_label"> Western Blot </span> <a class="ds-image overview-images data_ex_image_link" data-toggle="lightbox" data-gallery="overview-images-0" data-title="Detection of Human BMI‑1 by Western Blot." data-footer="Detection of Human BMI‑1 antibody by Western Blot." title="Cat. No. MAB33341 - Detection of Human BMI‑1 antibody by Western Blot." href="https://resources.rndsystems.com/images/datasheets/antibody/BMI-1_MAB33341_Western_Blot_6094.jpg"> <img loading="lazy" src="https://resources.rndsystems.com/images/datasheets/antibody/BMI-1_MAB33341_Western_Blot_6094.jpg" class="img-fluid" alt="Detection of Human BMI‑1 antibody by Western Blot." title="Detection of Human BMI‑1 antibody by Western Blot."> <span class="data_ex_image_larger">View Larger</span> </a> </div> </div> <div class="data_ex_description col-xs-12 col-sm-7 col-md-8"> <p> <span class="data_ex_description_title">Detection of Human BMI‑1 by Western Blot.</span> Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.1 µg/mL Mouse Anti-Human BMI-1 Monoclonal Antibody (Catalog # MAB33341) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class="NoLineLink" href="https://www.rndsystems.com/products/mouse-igg-horseradish-peroxidase-conjugated-antibody_haf007">HAF007</a>). A specific band for BMI-1 was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using <a class="NoLineLink" href="https://www.rndsystems.com/resources/technical/western-blot-conditions">Immunoblot Buffer Group 4</a>. </p> </div> </div> <div class="row data_ex_row"> <a id="ds_image_1" class="pageAnchor"></a> <div class="data_ex_image_wrapper col-xs-12 col-sm-5 col-md-4"> <div class="data_ex_image"> <span class="data_ex_image_label"> Chromatin Immunoprecipitation (ChIP) </span> <a class="ds-image overview-images data_ex_image_link" data-toggle="lightbox" data-gallery="overview-images-1" data-title="Detection of BMI‑1-regulated Genes by Chromatin Immunoprecipitation." data-footer="Detection of BMI-1-regulated Genes antibody by Chromatin Immunoprecipitation." title="Cat. No. MAB33341 - Detection of BMI-1-regulated Genes antibody by Chromatin Immunoprecipitation." href="https://resources.rndsystems.com/images/datasheets/antibody/BMI-1_MAB33341_Chromatin_Immunoprecipitation_11249.jpg"> <img loading="lazy" src="https://resources.rndsystems.com/images/datasheets/antibody/BMI-1_MAB33341_Chromatin_Immunoprecipitation_11249.jpg" class="img-fluid" alt="Detection of BMI-1-regulated Genes antibody by Chromatin Immunoprecipitation." title="Detection of BMI-1-regulated Genes antibody by Chromatin Immunoprecipitation."> <span class="data_ex_image_larger">View Larger</span> </a> </div> </div> <div class="data_ex_description col-xs-12 col-sm-7 col-md-8"> <p> <span class="data_ex_description_title">Detection of BMI‑1-regulated Genes by Chromatin Immunoprecipitation.</span> HeLa human cervical epithelial carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. BMI-1/DNA complexes were immunoprecipitated using 5 µg Mouse Anti-Human BMI-1 Monoclonal Antibody (Catalog # MAB33341) or control antibody (Catalog # <a class="NoLineLink" href="https://www.rndsystems.com/products/mouse-igg-2a-isotype-control_mab003">MAB003</a>) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Mouse IgG Secondary Antibody (Catalog # <a class="NoLineLink" href="https://www.rndsystems.com/products/goat-anti-mouse-igg-biotinylated-antibody_baf007">BAF007</a>). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # <a class="NoLineLink" href="https://www.rndsystems.com/products/magcellect-streptavidin-ferrofluid_mag999">MAG999</a>) and DNA was purified using chelating resin solution. The<EM>hoxc13</EM>promoter was detected by standard PCR. </p> </div> </div> <div class="row data_ex_row"> <a id="ds_image_2" class="pageAnchor"></a> <div class="data_ex_image_wrapper col-xs-12 col-sm-5 col-md-4"> <div class="data_ex_image"> <span class="data_ex_image_label"> Intracellular Staining by Flow Cytometry </span> <a class="ds-image overview-images data_ex_image_link" data-toggle="lightbox" data-gallery="overview-images-2" data-title="Detection of BMI‑1 in HeLa Human Cell Line by Flow Cytometry." data-footer="Detection of BMI‑1 antibody in HeLa Human Cell Line antibody by Flow Cytometry." title="Cat. No. MAB33341 - Detection of BMI‑1 antibody in HeLa Human Cell Line antibody by Flow Cytometry." href="https://resources.rndsystems.com/images/datasheets/antibody/BMI-1_MAB33341_Flow_Cytometry_8402.jpg"> <img loading="lazy" src="https://resources.rndsystems.com/images/datasheets/antibody/BMI-1_MAB33341_Flow_Cytometry_8402.jpg" class="img-fluid" alt="Detection of BMI‑1 antibody in HeLa Human Cell Line antibody by Flow Cytometry." title="Detection of BMI‑1 antibody in HeLa Human Cell Line antibody by Flow Cytometry."> <span class="data_ex_image_larger">View Larger</span> </a> </div> </div> <div class="data_ex_description col-xs-12 col-sm-7 col-md-8"> <p> <span class="data_ex_description_title">Detection of BMI‑1 in HeLa Human Cell Line by Flow Cytometry.</span> HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human BMI-1 Monoclonal Antibody (Catalog # MAB33341, filled histogram) or isotype control antibody (Catalog # <a class="NoLineLink" href="https://www.rndsystems.com/products/mouse-igg-2a-isotype-control_mab003">MAB003</a>, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')<sub>2</sub>Secondary Antibody (Catalog # <a class="NoLineLink" href="https://www.rndsystems.com/products/mouse-f-ab-2-igg-h-l-pe-conjugated-antibody_f0102b">F0102B</a>). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. </p> </div> </div> </div> </div> </div> <div id="ds_calculator" class="datasheet_section"> <div id="btn_calculator_wrapper"><div class="datasheet_calculator_toggle btn btn-secondary" id="btn_reconstitution">Reconstitution Calculator</div></div><div id="datasheet_calculators"><div id="btn_reconstitution_calc" class="datasheet_calculator"><h2>Reconstitution Calculator</h2><div id="reconstitution_calculator" class="calculator"> <form id="recCalc"> <div class="reconstitution_calculator_text" id="reconstitution_calculator_instructions"> <p>The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.</p> </div> <div class="calc_section"> <label for="reconstitution_calculator_volume" class="calculator_label">Volume (to add to vial)</label> <input id="reconstitution_calculator_volume" name="reconstitution_calculator_volume" type="text" maxlength="16" placeholder="volume" class="calculator_input calculator_text"/> <span class="select_wrapper"> <select id="reconstitution_calculator_volume_unit" class="calculator_input calculator_select"> <option value="ml">ml</option> <option value="ul" selected="selected">ul</option> </select> </span> </div> <span class="calculator_equals calculator_operator">=</span> <div class="calc_section"> <label for="reconstitution_calculator_mass" class="calculator_label">Mass (in vial)</label> <input id="reconstitution_calculator_mass" name="reconstitution_calculator_mass" type="text" maxlength="16" placeholder="mass" class="calculator_input calculator_text"/> <span class="select_wrapper"> <select id="reconstitution_calculator_mass_unit" class="calculator_input calculator_select"> <option value="mg">mg</option> <option value="ug" selected="selected">ug</option> <option value="ng">ng</option> </select> </span> </div> <span class="calculator_divide calculator_operator">÷</span> <div class="calc_section"> <label for="reconstitution_calculator_concentration" class="calculator_label">Desired Reconstitution Concentration</label> <input id="reconstitution_calculator_concentration" name="reconstitution_calculator_concentration" type="text" maxlength="16" placeholder="conc." class="calculator_input calculator_text"/> <span class="select_wrapper"> <select id="reconstitution_calculator_concentration_unit" class="calculator_input calculator_select"> <option value="mg/ml">mg/ml</option> <option value="ug/ml" selected="selected">ug/ml</option> <option value="ng/ml">ng/ml</option> </select> </span> </div> <div id="reconstitution_foot" class="calc_section"> <input type="submit" value="Calculate" id="button_reconstitution_calculator_calculate" class="calculator_button btn btn-secondary"/> </div> </form> </div> </div></div> </div> <div id="ds_preparation_storage" class="datasheet_section"> <h2>Preparation and Storage</h2> <div class="ds_table prep_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Reconstitution</div> <div class="ds_table_cell prep_table_data">Reconstitute at 0.5 mg/mL in sterile PBS. <div id="reconstitution-buffer"> <div id="reconstitution-buffer-placeholder"> <div class="et_spinner_small"></div> Loading... </div> </div> </div> </div> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Shipping</div> <div class="ds_table_cell prep_table_data">Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below. </div> </div> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Stability & Storage</div> <div class="ds_table_cell prep_table_data"><b>Use a manual defrost freezer and avoid repeated freeze-thaw cycles. </b><UL><LI>12 months from date of receipt, -20 to -70 °C as supplied.</LI><LI>1 month, 2 to 8 °C under sterile conditions after reconstitution.</LI><LI>6 months, -20 to -70 °C under sterile conditions after reconstitution.</LI></UL> </div> </div> </div> </div> </div> <div id="ds_background" class="datasheet_section"> <h2>Background: BMI-1</h2> <p><p>BMI-1 (<u>B</u> cell-specific <u>M</u>oloney-MLV <u>i</u>ntegration site #1) is a 45 kDa protooncogene that is a class II member of the Polycomb group of genes. It participates in the formation of a large multimeric complex termed PRC1 that inhibits target gene transcription. Loss of BMI-1 function precludes stem cells from self-replicating. Human BMI-1 contains an N-terminal RING-finger domain (aa 17-56), an NLS (aa 81-95) and a C-terminal Pro/Ser-rich region (aa 251-326). Human BMI-1 shares 99%, 97%, 99% and 99% aa sequence identity with bovine, mouse, feline and canine BMI-1, respectively.</p></p> <div class="ds_table background_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Long Name</div> <div class="ds_table_cell background_table_data">B Lymphoma Mo-MLV Insertion Region 1</div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Entrez Gene IDs</div> <div class="ds_table_cell background_table_data">648 (Human); 12151 (Mouse)</div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Alternate Names</div> <div class="ds_table_cell background_table_data">B lymphoma Mo-MLV insertion region 1 homolog (mouse); B lymphoma Mo-MLV insertion region 1 homolog; BMI1 polycomb ring finger oncogene; BMI1; BMI-1; FLVI2/BMI1; MGC12685; murine leukemia viral (bmi-1) oncogene homolog; PCGF4; polycomb complex protein BMI-1; polycomb group protein Bmi1; polycomb group ring finger 4; RING finger protein 51; RNF51; RNF51Polycomb group RING finger protein 4</div> </div> </div> </div> </div> <div class="datasheet_section" id="product-datasheets"> <h2 id="ds_datasheets">Product Datasheets</h2> <div class="product_datasheets"> <span class="sds-message hide"> <p class="sds-label">You must select a language.</p> <span class="close_sds_message">x</span> </span> <div class="ds_document"> <a id="cfpdf" href="//resources.rndsystems.com/pdfs/datasheets/mab33341.pdf?v=20241123" class="datasheets-link " target="_blank" rel="noreferrer nofollow">Product Datasheet</a> </div> <div class="ds_document"> <div class="ds_pdfData"><a class="datasheets-link" href="https://www.rndsystems.com/cofas/human-bmi-1-antibody-384515_mab33341">COA</a> </div> </div> <div class="ds_document sds_link"> <a class="sds_non_cf datasheets-link non_cf_download_link" target="_blank" rel="noreferrer" data-path="//aero.bio-techne.com/sds/pdf/mab33341?v=20241123">SDS</a> </div> </div> </div> <div id="ds_related_research_areas" class="datasheet_section"> <h2>Related Research Areas</h2> <ul class="ds_details"> <li> <a href="/research-area/adult-neurogenesis">Adult Neurogenesis</a> </li> <li> <a href="/research-area/cancer-biomarkers">Cancer Biomarkers</a> </li> <li> <a href="/research-area/cancer-stem-cell-markers">Cancer Stem Cell Markers</a> </li> <li> <a href="/research-area/epigenetics">Epigenetics</a> </li> <li> <a href="/research-area/epigenetics-and-emt">Epigenetics and EMT</a> </li> <li> <a href="/research-area/hematopoietic-stem-cell-markers">Hematopoietic Stem Cell Markers</a> </li> <li> <a href="/research-area/intestinal-stem-cell-markers">Intestinal Stem Cell Markers</a> </li> <li> <a href="/research-area/neural-stem-cell-markers">Neural Stem Cell Markers</a> </li> <li> <a href="/research-area/stem-cell-epigenetics">Stem Cell Epigenetics</a> </li> </ul> </div> </div> <div class="tab_content tab_3" data-tab="3" data-tabname="product-citations" id="citations"> <a id="citations_section"></a> <h2><span class="citations">Citation for Human BMI-1 Antibody</h2> <div id="citation"> <a id="dsTabCitations"></a> <p> R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. </p> <p> <strong><span id="maxcitations">1</span> Citation: Showing <span id="showfrom">1</span> - <span id="showto">1</span> </strong> </p> <div class="row"> <ol id="citations" class="col-xs-12" data-expandall="false" data-max-citations="1" data-max-show="10"> <li class="top-lined" data-species="Human" data-application="Western Blot" data-sampletype="Whole Cells"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/24571310"> BMI1 reprogrammes histone acetylation and enhances c-fos pathway via directly binding to Zmym3 in malignant myeloid progression. </a> </span><br/> Authors: Shen H, Chen Z, Ding X, Qi X, Cen J, Wang Y, Yao L, Chen Y<br/> J Cell Mol Med, 2014-02-27;18(6):1004-17.<br/> Species: Human<br/> Sample Types: Whole Cells<br/> Applications: Western Blot </li> </ol> </div> </div> </div> <div class="tab_content tab_4" data-tab="4" data-tabname="product-faqs" id="product-faqs"> <h2>FAQs</h2> <div class="row"> <div class="col-xs-12"> <p>No product specific FAQs exist for this product, however you may</p> <a class="btn btn-quinary view_all_faqs_btn" href="/resources/faqs/primary-antibodies"> View all Antibody FAQs </a> </div> </div> </div> <div class="tab_content tab_5" data-tab="5" data-tabname="product-supplemental" id="supplemental-products"> <div id="supplemental-products-tab-content-placeholder" class="tab-content-placeholder" data-id="supplemental-products" data-catalog_number="mab33341" data-language="en"> <div class="et_spinner_small"></div> Loading... </div> </div> <div class="tab_content tab_6" data-tab="6" data-tabname="product-reviews" id="reviews"> <h3>Reviews for Human BMI-1 Antibody</h3> <div class="row"> <div class="col-xs-12"> <div class="datasheet_section"> <div class="row"> <div class="col-md-6"> <p> Average Rating: 5 <span class="review_stars_5"></span> (Based on 1 Review) </p> <div class="review_rating_progress"> <div class="row"> <div class="col-xs-3"> 5 Star </div> <div class="col-xs-6"> <div class="progress"> <div class="progress-bar progress-bar-warning" role="progressbar" aria-valuenow="100" aria-valuemin="0" aria-valuemax="100" style="width:100%"> </div> </div> </div> <div class="col-xs-3"> 100% </div> </div> <div class="row"> <div class="col-xs-3"> 4 Star </div> <div class="col-xs-6"> <div class="progress"> <div class="progress-bar progress-bar-warning" role="progressbar" aria-valuenow="0" aria-valuemin="0" aria-valuemax="100" style="width:0%"> </div> </div> </div> <div class="col-xs-3"> 0% </div> </div> <div class="row"> <div class="col-xs-3"> 3 Star </div> <div class="col-xs-6"> <div class="progress"> <div class="progress-bar progress-bar-warning" role="progressbar" aria-valuenow="0" aria-valuemin="0" aria-valuemax="100" style="width:0%"> </div> </div> </div> <div class="col-xs-3"> 0% 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btn-quinary" href="/reviews/MAB33341"> Submit a Review </a> </div> </div> </div> </div> </div> </div> <div class="row"> <div class="col-md-12"> <div class="reviews_filters"> <p style="display:inline;"><b>Filter by:</b></p> <div class="select_wrapper"> <select class="review_filter_rating"> <option value="all">All Ratings</option> <option value="5">5</option> </select> </div> <div class="select_wrapper"> <select class="review_filter_application"> <option value="all">All Applications</option> <option value="WB"> WB </option> </select> </div> <div class="select_wrapper"> <select class="review_filter_species"> <option value="all">All Species</option> <option value="Human"> Human </option> </select> </div> </div> </div> </div> <br/> <div class="review_row" data-rating="5" data-species="Human" data-application="WB"> <div class="row"> <div class="col-xs-12 col-sm-10"> <div class="review_title review_stars_5">Human BMI-1 Antibody</div> <div class="review_date">By <span class="review_reviewer">Anonymous</span> on 03/22/2017 </div> <div class="row"> <div class="review_specifics col-xs-12"> <span class="ds_review_data"> <strong>Application:</strong> WB </span> <span class="ds_review_data"> <strong>Sample Tested: </strong> MCF-7 human breast cancer cell line </span> <span class="ds_review_data"><strong>Species:</strong> Human </span> </div> </div> <div class="review_how_used"><p>MCF-7 cell lysate probed with 1:2,500 Mouse anti-Human BMI-1 Antibody. Buffer: 5% BSA in PBS. Secondary Ab: anti-Mouse IgG 1:5,000. </p></div> </div> <div class="col-xs-12 col-sm-2"> <a class="ds-image" data-toggle="lightbox" href="https://www.rndsystems.com/sites/rndsystems.com/files/reviews/review_mab33341_7731.png" data-title="Western Blot Human BMI-1 Antibody MAB33341" data-gallery="Review-images"> <img loading="lazy" class="img-fluid review_image" alt="Western Blot Human BMI-1 Antibody MAB33341" title="Western Blot Human BMI-1 Antibody MAB33341" src="https://www.rndsystems.com/sites/rndsystems.com/files/reviews/review_mab33341_7731.png"/> </a> </div> </div> <hr/> </div> </div> </div> </div> <div id="sidebar" class="col-xs-12 col-sm-4 col-md-3 col-lg-3 col-xl-3"> <div class="distributor_region"></div> <div class="widget"> <div class="widget_title">Related Products</div> <div class="widget_content" id="related_products_area"> <ul class="ds_details"> <li> <a href="/target/bmi-1"> All Products for BMI-1 </a> </li> <li> <a href="/target/bmi-1?category=Primary%20Antibodies"> 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Human BMI-1 Antibody MAB33341: R&D Systems