Performance comparison of a previously validated microhaplotype panel and a forensic STR panel for DNA mixture analysis

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class="f18">☆</strong> <span class="f16">4.5</span> </span> <span class="mx-3"></span> <span class="tag">Article</span> </div> <h1 class="title title-for-article"> Performance comparison of a previously validated microhaplotype panel and a forensic STR panel for DNA mixture analysis </h1> <div class="help-links-left"> <p class="pub-info"> FORENSIC SCIENCE INTERNATIONAL-GENETICS (2025) </p> </div> </div> </div> <div class="container"> <div class="col-md-9 mt-3 px-0"> <div class="panel-box"> <h4>Related references <b class="text-primary"></b></h4> <span class="text-muted">Note: Only part of the references are listed.</span> <hr> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Medicine, Legal </span> </div> <h4> <a href="https://www.peeref.com/works/81566702" class="text-dark hover-underline">A tale of two PG systems: A comparison of the two most widely used continuous probabilistic genotyping systems in the United States</a> </h4> <p>Susan A. Greenspoon et al.</p> <div class="alert rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This article introduces two widely used probabilistic genotyping systems in the United States and compares their analysis results of 48 mock casework samples. The study found that the two systems produced the same results in approximately 91% of contributor-specific comparisons, but in cases where there were moderate or substantial differences in log(LR) values, 9% affected the conclusion of the reference association to the mixture. The article also discusses the possible reasons for the different results and notes that these deviations are usually predictable. </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">JOURNAL OF FORENSIC SCIENCES</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/81566702/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/27757489" class="text-dark hover-underline">An MPS-Based 50plex Microhaplotype Assay for Forensic DNA Analysis</a> </h4> <p>Ranran Zhang et al.</p> <div class="alert rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> In this study, a panel of 50 Microhaplotypes (MHs) was constructed and analyzed using the Multiseq multiple polymerase chain reaction (multi-PCR) targeted capture sequencing protocol. It was found that this panel has the advantages of both short tandem repeats (STRs) and single nucleotide polymorphisms (SNPs), with high sensitivity and specificity. It also showed measurable polymorphism among a group of Han individuals and had high detection rates for highly degraded samples and mixtures. </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">GENES</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/27757489/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/28372704" class="text-dark hover-underline">Development and evaluation of a novel panel containing 188 microhaplotypes for 2nd-degree kinship testing in the Hebei Han population</a> </h4> <p>Qingqing Du et al.</p> <div class="alert rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This is a research paper on distant kinship identification. The authors screened 188 MH markers from the 1000 Genomes Project database and constructed a new multiplex panel, which was evaluated. The results showed that the panel had good sequencing accuracy, high sensitivity, species specificity, and resistance to PCR inhibitors or degradation, and was suitable for individual identification and paternity testing. In addition, the authors also studied the efficiency of the panel in second- and third-degree kinship testing, and the results showed that the panel could effectively identify second-degree kinship, but for third-degree kinship identification, further research and improvement were needed. </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/28372704/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/28372733" class="text-dark hover-underline">Adapting an established Ampliseq microhaplotype panel to nanopore sequencing through direct PCR</a> </h4> <p>L. Casanova-Adan et al.</p> <div class="alert rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> We have successfully adapted the Ampliseq microhaplotype panel for forensic genetics applications using the Oxford Nanopore Technologies system. Our cost-effective and highly scalable solution combines direct PCR amplification with ONT library construction and sequencing. The results demonstrate stable coverage patterns, allele balance, and strand bias, achieving high profile completeness and concordance rates. The nanopore workflow also shows improved forensic utility compared to traditional methods. </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/28372733/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/25877859" class="text-dark hover-underline">A multipurpose panel of microhaplotypes for use with STR markers in casework</a> </h4> <p>Kenneth K. Kidd et al.</p> <div class="alert rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This article discusses a small panel of highly informative loci with potential for application in forensic casework. By comparing the A(e) and ancestry informativeness distributions of MHs with the augmented STR panel, it is found that MHs perform better in individualization and population differentiation. </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/25877859/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/26176438" class="text-dark hover-underline">A sequence-based 163plex microhaplotype assay for forensic DNA analysis</a> </h4> <p>Ruiyang Tao et al.</p> <div class="alert rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This study established a 163 microhaplotype (MH) multiplex assay and evaluated its performance and applicability. The results showed that the assay was accurate, repeatable, and reliable, and could be used for ancestry inference, human identification, paternity testing, and mixture deconvolution in forensic research. </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FRONTIERS IN GENETICS</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/26176438/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/19658368" class="text-dark hover-underline">Building a custom large-scale panel of novel microhaplotypes for forensic identification using MiSeq and Ion S5 massively parallel sequencing systems</a> </h4> <p>M. de la Puente et al.</p> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2020) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/19658368/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/19937825" class="text-dark hover-underline">A 124-plex Microhaplotype Panel Based on Next-generation Sequencing Developed for Forensic Applications</a> </h4> <p>Jing-Bo Pang et al.</p> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENTIFIC REPORTS</span> (2020) </p> <div class="flex-shrink-0"> <a class="btn 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Performance comparison of a previously validated microhaplotype panel and a forensic STR panel for DNA mixture analysis - Peeref