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<!-- views/paperById.ejs --> <!DOCTYPE html> <html> <head> <meta charset="utf-8"> <title>SCITEPRESS - SCIENCE AND TECHNOLOGY PUBLICATIONS</title> <meta name ="description" content="Digital Library" /> <meta name="citation_language" content="en"> <meta name="citation_title" content="N-acetylcysteine (NAC) Inhibits ROS-Induced Ferroptosis in CTNS Knockdown 尾-Cells in Vitro"> <meta name="citation_abstract" content="CTNS silence or mutation in the human body leads to cystinosis, an autosomal recessive lysosomal storage disease. Ferroptosis is an iron-dependent form of programmed cell death. N-acetylcysteine (NAC), a common antioxidant, is the acetylated precursor of L-cysteine. There are no studies about the relationship between cystinosis and ferroptosis yet. Therefore, in this paper, we aimed to 1) find out the relationship between CTNS knockdown and ferroptosis in 脽-cells and 2) verify that NAC is a potential agent to protect CTNS knockdown 脽-cells and is also a potent ferroptosis inhibitor. Since we do not have the access to do the experiments in a laboratory, all the following results and conclusions are hypothetical or from existing papers: CTNS-targeting siRNA inhibit cystinosin expression at mRNA and protein level; CTNS knockdown induces ferroptosis in 脽cells and NAC attenuates ferroptosis; NAC attenuates oxidative stress in CTNS knockdown 脽-cells; NAC restores energy level and glucose-stimulated insulin secretion in CTNS knockdown 脽-cells. We conclude that, in vitro, the NAC pretreatment can effectively rescue CTNS knockdown 脽-cells from ferroptosis by elevating the GPX4 mRNA and its protein level. "> <meta name="citation_publication_date" content="2022/02/25"> <meta name="citation_conference_title" content="The International Conference on Biomedical Engineering and Bioinformatics"> <meta name="citation_keywords" content="CTNS; Cystine; Ferroptosis; NAC; Insulin Secretion;"> <meta name="citation_doi" content="10.5220/0011390800003443"> <meta name="citation_isbn" content="978-989-758-595-1"> <meta name="citation_volume" content="2"> <meta name="citation_firstpage" content="1235"> <meta name="citation_lastpage" content="1243"> <meta name="citation_publisher" content="SCITEPRESS"> <meta name="citation_author" content="Hongrui Liang" > <meta name="citation_author_institution" content="China Agricultural University, Haidian District, Beijing 100089, China" > <meta name="citation_author" content="Jingyu Xu" > <meta name="citation_author_institution" content="Shanghai Foreign Language School, Shanghai 200083, China" > <meta name="citation_author" content="Yanxu Chen" > <meta name="citation_author_institution" content="Shanghai Starriver Bilingual School, Shanghai 201108, China" > <meta name="citation_author" content="Jingjie Yu" > <meta name="citation_author_institution" content="The Village School, Houston, TX, United States" > <meta name="citation_author" content="Ruoxun Peng" > <meta name="citation_author_institution" content="International Department, The Second High School Attached to Beijing Normal University, Beijing 100192, China" > <meta name="citation_abstract_html_url" content="/PublishedPapers/2022/113908"> <meta name="citation_pdf_url" content="/PublishedPapers/2022/113908/113908.pdf"> </head> <body> <article> <a href="/PublishedPapers/2022/113908/pdf/index.html"><h1 class="citation_title">N-acetylcysteine (NAC) Inhibits ROS-Induced Ferroptosis in CTNS Knockdown 尾-Cells in Vitro</h1></a> <h3 class="citation_author"> Hongrui Liang, Jingyu Xu, Yanxu Chen, Jingjie Yu, Ruoxun Peng</h3> <h4 class="citation_publication_date">2022</h4> <h4>Abstract</h4> <p class="citation_abstract">CTNS silence or mutation in the human body leads to cystinosis, an autosomal recessive lysosomal storage disease. Ferroptosis is an iron-dependent form of programmed cell death. N-acetylcysteine (NAC), a common antioxidant, is the acetylated precursor of L-cysteine. There are no studies about the relationship between cystinosis and ferroptosis yet. Therefore, in this paper, we aimed to 1) find out the relationship between CTNS knockdown and ferroptosis in 脽-cells and 2) verify that NAC is a potential agent to protect CTNS knockdown 脽-cells and is also a potent ferroptosis inhibitor. Since we do not have the access to do the experiments in a laboratory, all the following results and conclusions are hypothetical or from existing papers: CTNS-targeting siRNA inhibit cystinosin expression at mRNA and protein level; CTNS knockdown induces ferroptosis in 脽cells and NAC attenuates ferroptosis; NAC attenuates oxidative stress in CTNS knockdown 脽-cells; NAC restores energy level and glucose-stimulated insulin secretion in CTNS knockdown 脽-cells. We conclude that, in vitro, the NAC pretreatment can effectively rescue CTNS knockdown 脽-cells from ferroptosis by elevating the GPX4 mRNA and its protein level. </p> <a href="/PublishedPapers/2022/113908/113908.pdf" class="citation_pdf_url">Download</a> <br /> <br /> <br/> <h4 style="margin:0;">Paper Citation</h4> <br/> <h4 style="margin:0;">in Harvard Style</h4> <p style="margin:0;">Liang H., Xu J., Chen Y., Yu J. and Peng R. (2022). <b>N-acetylcysteine (NAC) Inhibits ROS-Induced Ferroptosis in CTNS Knockdown 尾-Cells in Vitro</b>. In <i>Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,</i> ISBN 978-989-758-595-1, pages 1235-1243. DOI: 10.5220/0011390800003443</p> <br/> <h4 style="margin:0;">in Bibtex Style</h4> <p style="margin:0;">@conference{icbeb22,<br />author={Hongrui Liang and Jingyu Xu and Yanxu Chen and Jingjie Yu and Ruoxun Peng},<br />title={N-acetylcysteine (NAC) Inhibits ROS-Induced Ferroptosis in CTNS Knockdown 尾-Cells in Vitro},<br />booktitle={Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,},<br />year={2022},<br />pages={1235-1243},<br />publisher={SciTePress},<br />organization={INSTICC},<br />doi={10.5220/0011390800003443},<br />isbn={978-989-758-595-1},<br />}</p> <br/> <h4 style="margin:0;">in EndNote Style</h4> <p style="margin:0;">TY - CONF <br /><br />JO - Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,<br />TI - N-acetylcysteine (NAC) Inhibits ROS-Induced Ferroptosis in CTNS Knockdown 尾-Cells in Vitro<br />SN - 978-989-758-595-1<br />AU - Liang H. <br />AU - Xu J. <br />AU - Chen Y. <br />AU - Yu J. <br />AU - Peng R. <br />PY - 2022<br />SP - 1235<br />EP - 1243<br />DO - 10.5220/0011390800003443<br /></p> <br/> </article> </body> </html>