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class="bg-surface-subtle" data-v-16104492><div class="m-container mx-auto w-full flex-col-reverse py-section-mobile pt-lg lg:py-3xl lg:border-b border-color-default" style="--container-ideal-width:92.5vw;" data-v-16104492><h3 class="m-heading text-inherit m-h3 font-semibold" data-v-16104492>76,577 preprints found</h3><div data-v-16104492><div class="m-search-select flex w-full flex-col gap-2 lg:flex-row lg:gap-0 pt-md" data-v-8ebcb524 data-v-16104492><div class="m-select common-field bg-white m-search-select__select min-w-[12.5rem] lg:!w-fit" data-v-8ebcb524><div class="common-field__wrapper" data-headlessui-state><div class="relative w-full cursor-default"><div class="flex items-center"><button id="headlessui-listbox-button-mui-43418" type="button" aria-haspopup="listbox" aria-expanded="false" data-headlessui-state data-testid="select-activator" class="common-field__dropdown-input [&>span]:overflow-hidden h-10"><span class="flex w-full items-center gap-1 [&>span]:truncate"><span>Author Name</span></span><div class="flex"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="keyboard_arrow_down" class="duration-400 transition-transform" style="" width="24" height="24" viewBox="0 0 16 16"></svg></div></button></div></div></div></div><div class="m-input common-field m-search-select__input" data-v-8ebcb524><div class="common-field__wrapper m-input__label"><div class="common-field__input flex items-center rounded h-10"><input class="h-full px-sm focus:outline-none pl-md" type="text" name="input" placeholder autocomplete="off" minlength="0" maxlength="524288" data-testid="m-input" value id="input-mui-43419"><span class="m-input__right-icon mr-md flex items-center justify-center"><button type="button" class="m-icon-button m-icon-button--md rounded -mr-2.5 bg-brand-sunken !p-xs text-color-brand-bold active:!bg-brand-subtlest" data-testid="m-select-search-search" data-v-8ebcb524><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="search" style="" width="24" height="24" viewBox="0 0 16 16"></svg><span class="sr-only">Search</span></button></span></div></div></div></div></div><div class="flex flex-col" data-v-16104492><div class="flex items-center pt-md match-type" data-v-16104492><p class="m-text text-sm pr-md" data-v-16104492>Match Type</p><div class="m-radio-group flex" id="headlessui-radiogroup-33316" role="radiogroup"><div class="group flex items-center gap-2 border-color-bold text-color-subtle transition-colors duration-75 cursor-pointer border-color-brand" id="headlessui-radiogroup-option-33317" role="radio" aria-checked="true" tabindex="0" data-headlessui-state="checked"><span class="relative flex items-center justify-center rounded-full border border-color-inherit transition-colors after:absolute after:h-1/2 after:w-1/2 after:rounded-full after:bg-brand-bold group-hover:after:bg-brand-bolder group-active:after:bg-brand-boldest" style="width:16px;height:16px;"></span><label id="headlessui-label-33318" class="text-sm text-color-black">Partial match</label></div><div class="group flex items-center gap-2 border-color-bold text-color-subtle transition-colors duration-75 cursor-pointer" id="headlessui-radiogroup-option-33319" role="radio" aria-checked="false" tabindex="-1" data-headlessui-state><span class="relative flex items-center justify-center rounded-full border border-color-inherit transition-colors after:absolute after:h-1/2 after:w-1/2 after:rounded-full" style="width:16px;height:16px;"></span><label id="headlessui-label-33320" class="text-sm text-color-black">Exact match</label></div></div></div><button class="m-button m-button--lg m-button--primary rounded mt-md !w-full lg:!hidden" type="button" data-v-16104492><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"> Search </span></button><button disabled class="m-button m-button--lg m-button--secondary m-button--disabled m-button--full-width rounded mt-sm lg:mt-md lg:!w-fit lg:order-first" type="button" data-v-16104492><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="add_circle" style="" width="16" height="16" viewBox="0 0 16 16" data-v-16104492></svg> Add Field </span></button><div class="hidden lg:flex justify-end items-center pt-md" data-v-16104492><button class="m-button m-button--lg m-button--tertiary rounded mr-md !text-color-subtle after:hidden" type="button" data-v-16104492><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"> Clear All </span></button><button class="m-button m-button--lg m-button--primary rounded" type="button" data-v-16104492><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"> Search </span></button></div></div></div></div><div class="m-container mx-auto m-container--percentages flex flex-col lg:flex-row gap-lg" style="--container-ideal-width:92.5%;"><div class="w-full lg:w-1/4"><button class="m-button m-button--lg m-button--secondary m-button--full-width rounded mt-sm lg:!hidden" type="button"><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="filter_list" weight="400" style="" width="24" height="24" viewBox="0 0 16 16"></svg>Filters</span></button><aside class="hidden w-full h-full pt-md pr-lg pb-7xl border-r border-color-default lg:block"><div class="lg:sticky sm:sticky sm:top-20 pr-md h-[80vh] overflow-auto"><div><div class="flex items-center justify-between"><div class="flex items-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="filter_list" style="" width="24" height="24" viewBox="0 0 16 16"></svg><p class="m-text text-sm font-semibold ml-xs">Filters</p></div><p class="m-text text-xs text-color-subtle text-right cursor-pointer">Clear All</p></div><div class="space-y-sm divide-y divide-color-default"><div class="pt-md"><div class="flex items-center justify-between"><p class="m-text text-sm font-semibold">Publication Period</p></div><div class="m-radio-group relative mt-md space-y-md" id="headlessui-radiogroup-33321" role="radiogroup"><div class="group flex items-center gap-2 border-color-bold text-color-subtle transition-colors duration-75 cursor-pointer" id="headlessui-radiogroup-option-33322" role="radio" aria-checked="false" tabindex="-1" data-headlessui-state><span class="relative flex items-center justify-center rounded-full border border-color-inherit transition-colors after:absolute after:h-1/2 after:w-1/2 after:rounded-full" style="width:16px;height:16px;"></span><label id="headlessui-label-33323" class="text-sm text-color-black"><span>Past 30 days</span><span class="absolute right-0 text-color-subtle">2,492 preprints</span></label></div><div class="group flex items-center gap-2 border-color-bold text-color-subtle transition-colors duration-75 cursor-pointer" id="headlessui-radiogroup-option-33324" role="radio" aria-checked="false" tabindex="-1" data-headlessui-state><span class="relative flex items-center justify-center rounded-full border border-color-inherit transition-colors after:absolute after:h-1/2 after:w-1/2 after:rounded-full" style="width:16px;height:16px;"></span><label id="headlessui-label-33325" class="text-sm text-color-black"><span>Past 6 months</span><span class="absolute right-0 text-color-subtle">14,435 preprints</span></label></div><div class="group flex items-center gap-2 border-color-bold text-color-subtle transition-colors duration-75 cursor-pointer" id="headlessui-radiogroup-option-33326" role="radio" aria-checked="false" tabindex="-1" data-headlessui-state><span class="relative flex items-center justify-center rounded-full border border-color-inherit transition-colors after:absolute after:h-1/2 after:w-1/2 after:rounded-full" style="width:16px;height:16px;"></span><label id="headlessui-label-33327" class="text-sm text-color-black"><span>Past 1 year</span><span class="absolute right-0 text-color-subtle">26,660 preprints</span></label></div></div></div><div class="pt-md"><div class="m-range-slider flex w-full flex-col gap-6 text-color-default gap-8"><div class="flex"><span class="text-sm font-medium" id="range-mui-43420">Publication Year</span><button class="m-range-slider__reset-button ml-auto text-xs text-color-subtlest hover:text-color-subtle">Reset</button></div><div class="flex w-full items-center gap-4"><span class="m-range-slider__indicator text-xs">2016</span><div class="w-full"><div class="m-range-slider__control"></div></div><span class="m-range-slider__indicator text-xs">2024</span></div></div></div><div class="pt-md"><div class="flex items-center justify-between"><p class="m-text text-sm font-semibold">Subject</p><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="remove" class="cursor-pointer" style="" width="16" height="16" viewBox="0 0 16 16"></svg></div><div class="pt-md cursor-pointer"><div class="flex"><label class="m-checkbox flex items-center gap-[0.65em] text-sm leading-[1.1] !text-color-white transition-colors duration-75 cursor-pointer flex-none"><input class="m-checkbox__control text-inherit m-0 grid h-[1.15em] w-[1.15em] cursor-pointer appearance-none place-content-center rounded-[0.15em] border-[0.15em] text-color-current !border-color-bold" type="checkbox" name="checkbox-input"></label><p class="m-text text-sm ml-sm">Arts and Humanities</p></div></div><div class="pt-md cursor-pointer"><div class="flex"><label class="m-checkbox flex items-center gap-[0.65em] text-sm leading-[1.1] !text-color-white transition-colors duration-75 cursor-pointer flex-none"><input class="m-checkbox__control text-inherit m-0 grid h-[1.15em] w-[1.15em] cursor-pointer appearance-none place-content-center rounded-[0.15em] border-[0.15em] text-color-current !border-color-bold" type="checkbox" name="checkbox-input"></label><p class="m-text text-sm ml-sm">Biology and Life Sciences</p></div></div><div class="pt-md cursor-pointer"><div class="flex"><label class="m-checkbox flex items-center gap-[0.65em] text-sm leading-[1.1] !text-color-white transition-colors duration-75 cursor-pointer flex-none"><input class="m-checkbox__control text-inherit m-0 grid h-[1.15em] w-[1.15em] cursor-pointer appearance-none place-content-center rounded-[0.15em] border-[0.15em] text-color-current !border-color-bold" type="checkbox" name="checkbox-input"></label><p class="m-text text-sm ml-sm">Business, Economics and Management</p></div></div><div class="pt-md cursor-pointer"><div class="flex"><label class="m-checkbox flex items-center gap-[0.65em] text-sm leading-[1.1] !text-color-white transition-colors duration-75 cursor-pointer flex-none"><input class="m-checkbox__control text-inherit m-0 grid h-[1.15em] w-[1.15em] cursor-pointer appearance-none place-content-center rounded-[0.15em] border-[0.15em] text-color-current !border-color-bold" type="checkbox" name="checkbox-input"></label><p class="m-text text-sm ml-sm">Chemistry and Materials Science</p></div></div><div class="pt-md cursor-pointer"><div class="flex"><label class="m-checkbox flex items-center gap-[0.65em] text-sm leading-[1.1] !text-color-white transition-colors duration-75 cursor-pointer flex-none"><input class="m-checkbox__control text-inherit m-0 grid h-[1.15em] w-[1.15em] cursor-pointer appearance-none place-content-center rounded-[0.15em] border-[0.15em] text-color-current !border-color-bold" type="checkbox" name="checkbox-input"></label><p class="m-text text-sm ml-sm">Computer Science and Mathematics</p></div></div><div class="pt-md cursor-pointer"><div class="flex"><label class="m-checkbox flex items-center gap-[0.65em] text-sm leading-[1.1] !text-color-white transition-colors duration-75 cursor-pointer flex-none"><input class="m-checkbox__control text-inherit m-0 grid h-[1.15em] w-[1.15em] cursor-pointer appearance-none place-content-center rounded-[0.15em] border-[0.15em] text-color-current !border-color-bold" type="checkbox" name="checkbox-input"></label><p class="m-text text-sm ml-sm">Engineering</p></div></div><div class="pt-md cursor-pointer"><div class="flex"><label class="m-checkbox flex items-center gap-[0.65em] text-sm leading-[1.1] !text-color-white transition-colors duration-75 cursor-pointer flex-none"><input class="m-checkbox__control text-inherit m-0 grid h-[1.15em] w-[1.15em] cursor-pointer appearance-none place-content-center rounded-[0.15em] border-[0.15em] text-color-current !border-color-bold" type="checkbox" name="checkbox-input"></label><p class="m-text text-sm ml-sm">Environmental and Earth Sciences</p></div></div><div class="pt-md cursor-pointer"><div class="flex"><label class="m-checkbox flex items-center gap-[0.65em] text-sm leading-[1.1] !text-color-white transition-colors duration-75 cursor-pointer flex-none"><input class="m-checkbox__control text-inherit m-0 grid h-[1.15em] w-[1.15em] cursor-pointer appearance-none place-content-center rounded-[0.15em] border-[0.15em] text-color-current !border-color-bold" type="checkbox" name="checkbox-input"></label><p class="m-text text-sm ml-sm">Medicine and Pharmacology</p></div></div><div class="pt-md cursor-pointer"><div class="flex"><label class="m-checkbox flex items-center gap-[0.65em] text-sm leading-[1.1] !text-color-white transition-colors duration-75 cursor-pointer flex-none"><input class="m-checkbox__control text-inherit m-0 grid h-[1.15em] w-[1.15em] cursor-pointer appearance-none place-content-center rounded-[0.15em] border-[0.15em] text-color-current !border-color-bold" type="checkbox" name="checkbox-input"></label><p class="m-text text-sm ml-sm">Physical Sciences</p></div></div><div class="pt-md cursor-pointer"><div class="flex"><label class="m-checkbox flex items-center gap-[0.65em] text-sm leading-[1.1] !text-color-white transition-colors duration-75 cursor-pointer flex-none"><input class="m-checkbox__control text-inherit m-0 grid h-[1.15em] w-[1.15em] cursor-pointer appearance-none place-content-center rounded-[0.15em] border-[0.15em] text-color-current !border-color-bold" type="checkbox" name="checkbox-input"></label><p class="m-text text-sm ml-sm">Public Health and Healthcare</p></div></div><div class="pt-md cursor-pointer"><div class="flex"><label class="m-checkbox flex items-center gap-[0.65em] text-sm leading-[1.1] !text-color-white transition-colors duration-75 cursor-pointer flex-none"><input class="m-checkbox__control text-inherit m-0 grid h-[1.15em] w-[1.15em] cursor-pointer appearance-none place-content-center rounded-[0.15em] border-[0.15em] text-color-current !border-color-bold" type="checkbox" name="checkbox-input"></label><p class="m-text text-sm ml-sm">Social Sciences</p></div></div></div><div class="pt-md"><div class="flex items-center justify-between"><p class="m-text text-sm font-semibold">Manuscript Type</p><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="remove" class="cursor-pointer" style="" width="16" height="16" viewBox="0 0 16 16"></svg></div><div class="mt-md mb-md space-y-md"><label class="m-checkbox flex items-center gap-[0.65em] text-sm leading-[1.1] !text-color-white transition-colors duration-75 cursor-pointer leading-normal"><input class="m-checkbox__control text-inherit m-0 grid h-[1.15em] w-[1.15em] cursor-pointer appearance-none place-content-center rounded-[0.15em] border-[0.15em] text-color-current 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16"></svg></span></button></div></div></div></div></aside></div><main class="flex-1 lg:min-h-screen pb-2xl lg:pb-7xl"><div class="flex justify-between lg:justify-end items-center pb-md lg:pt-md border-b border-color-default"><p class="m-text text-sm text-color-subtlest mr-sm">Sort by </p><div class="m-select common-field bg-white !w-40 min-w-[146px]"><div class="common-field__wrapper" data-headlessui-state><div class="relative w-full cursor-default"><div class="flex items-center"><button id="headlessui-listbox-button-mui-43421" type="button" aria-haspopup="listbox" aria-expanded="false" data-headlessui-state data-testid="select-activator" class="common-field__dropdown-input [&>span]:overflow-hidden h-10"><span class="flex w-full items-center gap-1 [&>span]:truncate"><span>Most Views</span></span><div class="flex"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="keyboard_arrow_down" class="duration-400 transition-transform" style="" width="24" height="24" viewBox="0 0 16 16"></svg></div></button></div></div></div></div></div><div data-v-891fb557><div class="border-b border-color-default pb-md mb-sm" data-v-891fb557><div class="pt-md lg:pt-lg lg:flex justify-between space-y-xs" data-v-891fb557><div class="flex flex-wrap gap-x-xs gap-y-xs" data-v-891fb557><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 bg-content-subtler text-color-default px-2 !bg-surface-disabled" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Review</span></div><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 border border-color-bold px-2 text-color-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Immunology and Allergy</span></div><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 border border-color-bold px-2 text-color-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Medicine and Pharmacology</span></div></div><div class="flex" data-v-891fb557><button class="m-button m-button--sm m-button--tertiary rounded mr-md after:hidden" type="button" data-v-891fb557><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="download" style="" width="16" height="16" viewBox="0 0 16 16" data-v-891fb557></svg> Download PDF </span></button><button class="m-button m-button--sm m-button--tertiary rounded after:hidden" type="button" data-v-891fb557><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="format_quote" style="" width="16" height="16" viewBox="0 0 16 16" data-v-891fb557></svg> Cite </span></button></div></div><a href="/manuscript/202004.0203/v4" class="" data-v-891fb557><h5 class="m-heading text-inherit m-h5 font-normal pt-sm hover:text-color-link-bold" data-v-891fb557>Face Masks Against COVID-19: An Evidence Review</h5></a><div class="flex pt-sm flex-wrap gap-sm" data-v-891fb557><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Jeremy Howard<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Austin Huang<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Zhiyuan Li<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Zeynep Tufekci<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Vladimir Zdimal<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Helene-Mari van der Westhuizen<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Arne von Delft<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Amy Price<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Lex Fridman<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Lei-Han Tang</p></div><div data-v-891fb557><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 bg-content-subtler text-color-default px-2 bg-info-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">+9 authors</span></div></div></div><div class="w-full relative overflow-hidden pt-sm" data-v-891fb557><div><div class="clamp-3"><div class="inline abstract-content" data-v-891fb557>Abstract: The science around the use of masks by the general public to impede COVID-19 transmission is advancing rapidly. Policymakers need guidance on how masks should be used by the general population to combat the COVID-19 pandemic. In this narrative review, we develop an analytical framework to examine mask usage, considering and synthesizing the relevant literature to inform multiple areas: population impact; transmission characteristics; source control; PPE; sociological considerations; and implementation considerations. A primary route of transmission of COVID-19 is via respiratory droplets, and is known to be transmissible from presymptomatic and asymptomatic individuals. Reducing disease spread requires two things: first, limit contacts of infected individuals via physical distancing and other measures, and second, reduce the transmission probability per contact. The preponderance of evidence indicates that mask wearing reduces the transmissibility per contact by reducing transmission of infected droplets in both laboratory and clinical contexts. Public mask wearing is most effective at reducing spread of the virus when compliance is high. The decreased transmissibility could substantially reduce the death toll and economic impact while the cost of the intervention is low. Given the current shortages of medical masks we recommend the adoption of public cloth mask wearing, as an effective form of source control, in conjunction with existing hygiene, distancing, and contact tracing strategies. Because many respiratory droplets become smaller due to evaporation, we recommend increasing focus on a previously overlooked aspect of mask usage: mask-wearing by infectious people ("source control") with benefits at the population-level, rather than mask-wearing by susceptible people, such as health-care workers, with focus on individual outcomes. We recommend that public officials and governments strongly encourage the use of widespread face masks in public, including the use of appropriate regulation.</div></div><div class="invisible absolute"><div class="inline abstract-content" data-v-891fb557>Abstract: The science around the use of masks by the general public to impede COVID-19 transmission is advancing rapidly. Policymakers need guidance on how masks should be used by the general population to combat the COVID-19 pandemic. In this narrative review, we develop an analytical framework to examine mask usage, considering and synthesizing the relevant literature to inform multiple areas: population impact; transmission characteristics; source control; PPE; sociological considerations; and implementation considerations. A primary route of transmission of COVID-19 is via respiratory droplets, and is known to be transmissible from presymptomatic and asymptomatic individuals. Reducing disease spread requires two things: first, limit contacts of infected individuals via physical distancing and other measures, and second, reduce the transmission probability per contact. The preponderance of evidence indicates that mask wearing reduces the transmissibility per contact by reducing transmission of infected droplets in both laboratory and clinical contexts. Public mask wearing is most effective at reducing spread of the virus when compliance is high. The decreased transmissibility could substantially reduce the death toll and economic impact while the cost of the intervention is low. Given the current shortages of medical masks we recommend the adoption of public cloth mask wearing, as an effective form of source control, in conjunction with existing hygiene, distancing, and contact tracing strategies. Because many respiratory droplets become smaller due to evaporation, we recommend increasing focus on a previously overlooked aspect of mask usage: mask-wearing by infectious people ("source control") with benefits at the population-level, rather than mask-wearing by susceptible people, such as health-care workers, with focus on individual outcomes. We recommend that public officials and governments strongly encourage the use of widespread face masks in public, including the use of appropriate regulation.</div></div></div><div style="display:none;" class="absolute right-0 bottom-0"><span><span class="inline-block align-middle w-6 h-4 bg-gradient-to-r from-transparent to-white"></span><span class="bg-white">... </span></span><button class="m-button m-button--sm m-button--tertiary rounded !inline-flex after:hidden bg-white" type="button"><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center">Show more <svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="keyboard_arrow_down" style="" width="16" height="16" viewBox="0 0 16 16"></svg></span></button></div></div><div class="pt-sm text-color-subtle flex gap-x-sm gap-y-sm flex-col lg:flex-row" data-v-891fb557><p class="m-text text-sm" data-v-891fb557> Posted: 02 November 2020</p><p class="m-text text-sm underline cursor-pointer" data-v-891fb557><a href="/manuscript/202004.0203/v4" data-v-891fb557><span data-v-891fb557>https://doi.org/</span>10.20944/preprints202004.0203.v4</a></p></div></div><div data-v-891fb557></div></div><div data-v-891fb557><div class="border-b border-color-default pb-md mb-sm" data-v-891fb557><div class="pt-md lg:pt-lg lg:flex justify-between space-y-xs" data-v-891fb557><div class="flex flex-wrap gap-x-xs gap-y-xs" data-v-891fb557><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 bg-content-subtler text-color-default px-2 !bg-surface-disabled" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Article</span></div><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 border border-color-bold px-2 text-color-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Pharmacy</span></div><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 border border-color-bold px-2 text-color-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Medicine and Pharmacology</span></div></div><div class="flex" data-v-891fb557><button class="m-button m-button--sm m-button--tertiary rounded mr-md after:hidden" type="button" data-v-891fb557><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="download" style="" width="16" height="16" viewBox="0 0 16 16" data-v-891fb557></svg> Download PDF </span></button><button class="m-button m-button--sm m-button--tertiary rounded after:hidden" type="button" data-v-891fb557><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="format_quote" style="" width="16" height="16" viewBox="0 0 16 16" data-v-891fb557></svg> Cite </span></button></div></div><a href="/manuscript/202007.0025/v1" class="" data-v-891fb557><h5 class="m-heading text-inherit m-h5 font-normal pt-sm hover:text-color-link-bold" data-v-891fb557>COVID-19 Outpatients – Early Risk-Stratified Treatment with Zinc Plus Low Dose Hydroxychloroquine and Azithromycin: A Retrospective Case Series Study</h5></a><div class="flex pt-sm flex-wrap gap-sm" data-v-891fb557><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Martin Scholz<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Roland Derwand<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Vladimir Zelenko</p></div></div><div class="w-full relative overflow-hidden pt-sm" data-v-891fb557><div><div class="clamp-3"><div class="inline abstract-content" data-v-891fb557>Abstract: <em>Objective: </em>To describe outcomes of patients with coronavirus disease 2019 (COVID-19) in the outpatient setting after early treatment with zinc, low dose hydroxychloroquine, and azithromycin (the triple therapy) dependent on risk stratification. <em>Design:</em> Retrospective case series study. <em>Setting:</em> General practice. <em>Participants:</em> 141 COVID-19 patients with laboratory confirmed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections in the year 2020. <em>Main Outcome Measures:</em> Risk-stratified treatment decision, rate of hospitalization and all-cause death. <em>Results: </em>Of 335 positively PCR-tested COVID-19 patients, 127 were treated with the triple therapy. 104 of 127 met the defined risk stratification criteria and were included in the analysis. In addition, 37 treated and eligible patients who were confirmed by IgG tests were included in the treatment group (total N=141). 208 of the 335 patients did not meet the risk stratification criteria and were not treated. After 4 days (median, IQR 3-6, available for N=66/141) of onset of symptoms, 141 patients (median age 58 years, IQR 40-67; 73% male) got a prescription for the triple therapy for 5 days. Independent public reference data from 377 confirmed COVID-19 patients of the same community were used as untreated control. 4 of 141 treated patients (2.8%) were hospitalized, which was significantly less (p<0.001) compared with 58 of 377 untreated patients (15.4%) (odds ratio 0.16, 95% CI 0.06-0.5). Therefore, the odds of hospitalization of treated patients were 84% less than in the untreated group. One patient (0.7%) died in the treatment group versus 13 patients (3.5%) in the untreated group (odds ratio 0.2, 95% CI 0.03-1.5; p=0.16). There were no cardiac side effects. <em>Conclusions:</em> Risk stratification-based treatment of COVID-19 outpatients as early as possible after symptom onset with the used triple therapy, including the combination of zinc with low dose hydroxychloroquine, was associated with significantly less hospitalizations and 5 times less all-cause deaths.</div></div><div class="invisible absolute"><div class="inline abstract-content" data-v-891fb557>Abstract: <em>Objective: </em>To describe outcomes of patients with coronavirus disease 2019 (COVID-19) in the outpatient setting after early treatment with zinc, low dose hydroxychloroquine, and azithromycin (the triple therapy) dependent on risk stratification. <em>Design:</em> Retrospective case series study. <em>Setting:</em> General practice. <em>Participants:</em> 141 COVID-19 patients with laboratory confirmed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections in the year 2020. <em>Main Outcome Measures:</em> Risk-stratified treatment decision, rate of hospitalization and all-cause death. <em>Results: </em>Of 335 positively PCR-tested COVID-19 patients, 127 were treated with the triple therapy. 104 of 127 met the defined risk stratification criteria and were included in the analysis. In addition, 37 treated and eligible patients who were confirmed by IgG tests were included in the treatment group (total N=141). 208 of the 335 patients did not meet the risk stratification criteria and were not treated. After 4 days (median, IQR 3-6, available for N=66/141) of onset of symptoms, 141 patients (median age 58 years, IQR 40-67; 73% male) got a prescription for the triple therapy for 5 days. Independent public reference data from 377 confirmed COVID-19 patients of the same community were used as untreated control. 4 of 141 treated patients (2.8%) were hospitalized, which was significantly less (p<0.001) compared with 58 of 377 untreated patients (15.4%) (odds ratio 0.16, 95% CI 0.06-0.5). Therefore, the odds of hospitalization of treated patients were 84% less than in the untreated group. One patient (0.7%) died in the treatment group versus 13 patients (3.5%) in the untreated group (odds ratio 0.2, 95% CI 0.03-1.5; p=0.16). There were no cardiac side effects. <em>Conclusions:</em> Risk stratification-based treatment of COVID-19 outpatients as early as possible after symptom onset with the used triple therapy, including the combination of zinc with low dose hydroxychloroquine, was associated with significantly less hospitalizations and 5 times less all-cause deaths.</div></div></div><div style="display:none;" class="absolute right-0 bottom-0"><span><span class="inline-block align-middle w-6 h-4 bg-gradient-to-r from-transparent to-white"></span><span class="bg-white">... </span></span><button class="m-button m-button--sm m-button--tertiary rounded !inline-flex after:hidden bg-white" type="button"><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center">Show more <svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="keyboard_arrow_down" style="" width="16" height="16" viewBox="0 0 16 16"></svg></span></button></div></div><div class="pt-sm text-color-subtle flex gap-x-sm gap-y-sm flex-col lg:flex-row" data-v-891fb557><p class="m-text text-sm" data-v-891fb557> Posted: 03 July 2020</p><p class="m-text text-sm underline cursor-pointer" data-v-891fb557><a href="/manuscript/202007.0025/v1" data-v-891fb557><span data-v-891fb557>https://doi.org/</span>10.20944/preprints202007.0025.v1</a></p></div></div><div data-v-891fb557></div></div><div data-v-891fb557><div class="border-b border-color-default pb-md mb-sm" data-v-891fb557><div class="pt-md lg:pt-lg lg:flex justify-between space-y-xs" data-v-891fb557><div class="flex flex-wrap gap-x-xs gap-y-xs" data-v-891fb557><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 bg-content-subtler text-color-default px-2 !bg-surface-disabled" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Article</span></div><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 border border-color-bold px-2 text-color-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Biochemistry and Molecular Biology</span></div><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 border border-color-bold px-2 text-color-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Biology and Life Sciences</span></div></div><div class="flex" data-v-891fb557><button class="m-button m-button--sm m-button--tertiary rounded mr-md after:hidden" type="button" data-v-891fb557><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="download" style="" width="16" height="16" viewBox="0 0 16 16" data-v-891fb557></svg> Download PDF </span></button><button class="m-button m-button--sm m-button--tertiary rounded after:hidden" type="button" data-v-891fb557><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="format_quote" style="" width="16" height="16" viewBox="0 0 16 16" data-v-891fb557></svg> Cite </span></button></div></div><a href="/manuscript/202004.0315/v1" class="" data-v-891fb557><h5 class="m-heading text-inherit m-h5 font-normal pt-sm hover:text-color-link-bold" data-v-891fb557>In Search of Preventative Strategies: Novel Anti-Inflammatory High-CBD <em>Cannabis Sativa</em> Extracts Modulate ACE2 Expression in COVID-19 Gateway Tissues</h5></a><div class="flex pt-sm flex-wrap gap-sm" data-v-891fb557><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Bo Wang<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Anna Kovalchuk<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Dongping Li<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Yaroslav Ilnytskyy<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Igor Kovalchuk<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Olga Kovalchuk</p></div></div><div class="w-full relative overflow-hidden pt-sm" data-v-891fb557><div><div class="clamp-3"><div class="inline abstract-content" data-v-891fb557>Abstract: With the rapidly growing pandemic of COVID-19 caused by the new and challenging to treat zoonotic SARS-CoV2 coronavirus, there is an urgent need for new therapies and prevention strategies that can help curtail disease spread and reduce mortality. Inhibition of viral entry and thereby spread constitute plausible therapeutic avenues. Similar to other respiratory pathogens, SARS-CoV2 is transmitted through respiratory droplets, with potential for aerosol and contact spread. It uses receptor-mediated entry into the human host via angiotensin-converting enzyme II (ACE2) that is expressed in lung tissue, as well as oral and nasal mucosa, kidney, testes, and the gastrointestinal tract. Modulation of ACE2 levels in these gateway tissues may prove a plausible strategy for decreasing disease susceptibility. Cannabis sativa, especially one high in the anti-inflammatory cannabinoid cannabidiol (CBD), has been proposed to modulate gene expression and inflammation and harbour anti-cancer and anti-inflammatory properties. Working under the Health Canada research license, we have developed over 800 new Cannabis sativa lines and extracts and hypothesized that high-CBD C. sativa extracts may be used to modulate ACE2 expression in COVID-19 target tissues. Screening C. sativa extracts using artificial human 3D models of oral, airway, and intestinal tissues, we identified 13 high CBD C. sativa extracts that modulate ACE2 gene expression and ACE2 protein levels. Our initial data suggest that some C. sativa extract down-regulate serine protease TMPRSS2, another critical protein required for SARS-CoV2 entry into host cells. While our most effective extracts require further large-scale validation, our study is crucial for the future analysis of the effects of medical cannabis on COVID-19. The extracts of our most successful and novel high CBD C. sativa lines, pending further investigation, may become a useful and safe addition to the treatment of COVID-19 as an adjunct therapy. They can be used to develop easy-to-use preventative treatments in the form of mouthwash and throat gargle products for both clinical and at-home use. Such products ought to be tested for their potential to decrease viral entry via the oral mucosa. Given the current dire and rapidly evolving epidemiological situation, every possible therapeutic opportunity and avenue must be considered.</div></div><div class="invisible absolute"><div class="inline abstract-content" data-v-891fb557>Abstract: With the rapidly growing pandemic of COVID-19 caused by the new and challenging to treat zoonotic SARS-CoV2 coronavirus, there is an urgent need for new therapies and prevention strategies that can help curtail disease spread and reduce mortality. Inhibition of viral entry and thereby spread constitute plausible therapeutic avenues. Similar to other respiratory pathogens, SARS-CoV2 is transmitted through respiratory droplets, with potential for aerosol and contact spread. It uses receptor-mediated entry into the human host via angiotensin-converting enzyme II (ACE2) that is expressed in lung tissue, as well as oral and nasal mucosa, kidney, testes, and the gastrointestinal tract. Modulation of ACE2 levels in these gateway tissues may prove a plausible strategy for decreasing disease susceptibility. Cannabis sativa, especially one high in the anti-inflammatory cannabinoid cannabidiol (CBD), has been proposed to modulate gene expression and inflammation and harbour anti-cancer and anti-inflammatory properties. Working under the Health Canada research license, we have developed over 800 new Cannabis sativa lines and extracts and hypothesized that high-CBD C. sativa extracts may be used to modulate ACE2 expression in COVID-19 target tissues. Screening C. sativa extracts using artificial human 3D models of oral, airway, and intestinal tissues, we identified 13 high CBD C. sativa extracts that modulate ACE2 gene expression and ACE2 protein levels. Our initial data suggest that some C. sativa extract down-regulate serine protease TMPRSS2, another critical protein required for SARS-CoV2 entry into host cells. While our most effective extracts require further large-scale validation, our study is crucial for the future analysis of the effects of medical cannabis on COVID-19. The extracts of our most successful and novel high CBD C. sativa lines, pending further investigation, may become a useful and safe addition to the treatment of COVID-19 as an adjunct therapy. They can be used to develop easy-to-use preventative treatments in the form of mouthwash and throat gargle products for both clinical and at-home use. Such products ought to be tested for their potential to decrease viral entry via the oral mucosa. Given the current dire and rapidly evolving epidemiological situation, every possible therapeutic opportunity and avenue must be considered.</div></div></div><div style="display:none;" class="absolute right-0 bottom-0"><span><span class="inline-block align-middle w-6 h-4 bg-gradient-to-r from-transparent to-white"></span><span class="bg-white">... </span></span><button class="m-button m-button--sm m-button--tertiary rounded !inline-flex after:hidden bg-white" type="button"><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center">Show more <svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="keyboard_arrow_down" style="" width="16" height="16" viewBox="0 0 16 16"></svg></span></button></div></div><div class="pt-sm text-color-subtle flex gap-x-sm gap-y-sm flex-col lg:flex-row" data-v-891fb557><p class="m-text text-sm" data-v-891fb557> Posted: 19 April 2020</p><p class="m-text text-sm underline cursor-pointer" data-v-891fb557><a href="/manuscript/202004.0315/v1" data-v-891fb557><span data-v-891fb557>https://doi.org/</span>10.20944/preprints202004.0315.v1</a></p></div></div><div data-v-891fb557></div></div><div data-v-891fb557><div class="border-b border-color-default pb-md mb-sm" data-v-891fb557><div class="pt-md lg:pt-lg lg:flex justify-between space-y-xs" data-v-891fb557><div class="flex flex-wrap gap-x-xs gap-y-xs" data-v-891fb557><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 bg-content-subtler text-color-default px-2 !bg-surface-disabled" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Article</span></div><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 border border-color-bold px-2 text-color-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Cardiac and Cardiovascular Systems</span></div><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 border border-color-bold px-2 text-color-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Medicine and Pharmacology</span></div></div><div class="flex" data-v-891fb557><button class="m-button m-button--sm m-button--tertiary rounded mr-md after:hidden" type="button" data-v-891fb557><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="download" style="" width="16" height="16" viewBox="0 0 16 16" data-v-891fb557></svg> Download PDF </span></button><button class="m-button m-button--sm m-button--tertiary rounded after:hidden" type="button" data-v-891fb557><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="format_quote" style="" width="16" height="16" viewBox="0 0 16 16" data-v-891fb557></svg> Cite </span></button></div></div><a href="/manuscript/202208.0151/v1" class="" data-v-891fb557><h5 class="m-heading text-inherit m-h5 font-normal pt-sm hover:text-color-link-bold" data-v-891fb557>Cardiovascular Effects of the BNT162b2 mRNA COVID-19 Vaccine in Adolescents</h5></a><div class="flex pt-sm flex-wrap gap-sm" data-v-891fb557><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Suyanee Mansanguan<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Prakaykaew Charunwatthana<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Watcharapong Piyaphanee<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Wilanee Dechkhajorn<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Akkapon Poolcharoen<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Chayasin Mansanguan</p></div></div><div class="w-full relative overflow-hidden pt-sm" data-v-891fb557><div><div class="clamp-3"><div class="inline abstract-content" data-v-891fb557>Abstract: This study focuses on cardiovascular effects, particularly myocarditis and pericarditis events, after BNT162b2 mRNA COVID-19 vaccine injection in Thai adolescents. This prospective cohort study enrolled students from two schools aged 13–18 years who received the second dose of the BNT162b2 mRNA COVID-19 vaccine. Data including demographics, symptoms, vital signs, ECG, echocardiography and cardiac enzymes were collected at baseline, Day 3, Day 7, and Day 14 (optional) using case record forms.We enrolled 314 participants; of these, 13 participants were lost to follow up, leaving 301 participants for analysis. The most common cardiovascular effects were tachycardia (7.64%), shortness of breath (6.64%), palpitation (4.32%), chest pain (4.32%), and hypertension (3.99%). Seven participants (2.33%) exhibited at least one elevated cardiac biomarker or positive lab assessments. Cardiovascular effects were found in 29.24% of patients, ranging from tachycardia, palpitation, and myopericarditis. Myopericarditis was confirmed in one patient after vaccination. Two patients had suspected pericarditis and four patients had suspected subclinical myocarditis. Conclusion: Cardiovascular effects in adolescents after BNT162b2 mRNA COVID-19 vaccination included tachycardia, palpitation, and myocarditis. The clinical presentation of myopericarditis after vaccination was usually mild, with all cases fully recovering within 14 days. Hence, adolescents receiving mRNA vaccines should be monitored for side effects. Clinical Trial Registration: NCT05288231</div></div><div class="invisible absolute"><div class="inline abstract-content" data-v-891fb557>Abstract: This study focuses on cardiovascular effects, particularly myocarditis and pericarditis events, after BNT162b2 mRNA COVID-19 vaccine injection in Thai adolescents. This prospective cohort study enrolled students from two schools aged 13–18 years who received the second dose of the BNT162b2 mRNA COVID-19 vaccine. Data including demographics, symptoms, vital signs, ECG, echocardiography and cardiac enzymes were collected at baseline, Day 3, Day 7, and Day 14 (optional) using case record forms.We enrolled 314 participants; of these, 13 participants were lost to follow up, leaving 301 participants for analysis. The most common cardiovascular effects were tachycardia (7.64%), shortness of breath (6.64%), palpitation (4.32%), chest pain (4.32%), and hypertension (3.99%). Seven participants (2.33%) exhibited at least one elevated cardiac biomarker or positive lab assessments. Cardiovascular effects were found in 29.24% of patients, ranging from tachycardia, palpitation, and myopericarditis. Myopericarditis was confirmed in one patient after vaccination. Two patients had suspected pericarditis and four patients had suspected subclinical myocarditis. Conclusion: Cardiovascular effects in adolescents after BNT162b2 mRNA COVID-19 vaccination included tachycardia, palpitation, and myocarditis. The clinical presentation of myopericarditis after vaccination was usually mild, with all cases fully recovering within 14 days. Hence, adolescents receiving mRNA vaccines should be monitored for side effects. Clinical Trial Registration: NCT05288231</div></div></div><div style="display:none;" class="absolute right-0 bottom-0"><span><span class="inline-block align-middle w-6 h-4 bg-gradient-to-r from-transparent to-white"></span><span class="bg-white">... </span></span><button class="m-button m-button--sm m-button--tertiary rounded !inline-flex after:hidden bg-white" type="button"><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center">Show more <svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="keyboard_arrow_down" style="" width="16" height="16" viewBox="0 0 16 16"></svg></span></button></div></div><div class="pt-sm text-color-subtle flex gap-x-sm gap-y-sm flex-col lg:flex-row" data-v-891fb557><p class="m-text text-sm" data-v-891fb557> Posted: 08 August 2022</p><p class="m-text text-sm underline cursor-pointer" data-v-891fb557><a href="/manuscript/202208.0151/v1" data-v-891fb557><span data-v-891fb557>https://doi.org/</span>10.20944/preprints202208.0151.v1</a></p></div></div><div data-v-891fb557></div></div><div data-v-891fb557><div class="border-b border-color-default pb-md mb-sm" data-v-891fb557><div class="pt-md lg:pt-lg lg:flex justify-between space-y-xs" data-v-891fb557><div class="flex flex-wrap gap-x-xs gap-y-xs" data-v-891fb557><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 bg-content-subtler text-color-default px-2 !bg-surface-disabled" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Article</span></div><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 border border-color-bold px-2 text-color-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Insect Science</span></div><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 border border-color-bold px-2 text-color-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Biology and Life Sciences</span></div></div><div class="flex" data-v-891fb557><button class="m-button m-button--sm m-button--tertiary rounded mr-md after:hidden" type="button" data-v-891fb557><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="download" style="" width="16" height="16" viewBox="0 0 16 16" data-v-891fb557></svg> Download PDF </span></button><button class="m-button m-button--sm m-button--tertiary rounded after:hidden" type="button" data-v-891fb557><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="format_quote" style="" width="16" height="16" viewBox="0 0 16 16" data-v-891fb557></svg> Cite </span></button></div></div><a href="/manuscript/202101.0412/v2" class="" data-v-891fb557><h5 class="m-heading text-inherit m-h5 font-normal pt-sm hover:text-color-link-bold" data-v-891fb557>Raspberry PI for Kill Mosquitoes by Laser</h5></a><div class="flex pt-sm flex-wrap gap-sm" data-v-891fb557><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Ildar Rakhmatulin</p></div></div><div class="w-full relative overflow-hidden pt-sm" data-v-891fb557><div><div class="clamp-3"><div class="inline abstract-content" data-v-891fb557>Abstract: More than 700 thousand human deaths from mosquito bites are observed annually in the world. It is more than 2 times the number of annual murders in the world. In this regard, the invention of new more effective methods of protection against mosquitoes is necessary. In this article for the first time, comprehensive studies of mosquito neutralization using machine vision and a 1 W power laser are considered. Developed laser installation with Raspberry Pi that changing the direction of the laser with a galvanometer. We developed a program for mosquito tracking in real. The possibility of using deep neural networks, Haar cascades, machine learning for mosquito recognition was considered. We considered in detail the classification problems of mosquitoes in images. A recommendation is given for the implementation of this device based on a microcontroller for subsequent use as part of an unmanned aerial vehicle. Any harmful insects in the fields can be used as objects for control.</div></div><div class="invisible absolute"><div class="inline abstract-content" data-v-891fb557>Abstract: More than 700 thousand human deaths from mosquito bites are observed annually in the world. It is more than 2 times the number of annual murders in the world. In this regard, the invention of new more effective methods of protection against mosquitoes is necessary. In this article for the first time, comprehensive studies of mosquito neutralization using machine vision and a 1 W power laser are considered. Developed laser installation with Raspberry Pi that changing the direction of the laser with a galvanometer. We developed a program for mosquito tracking in real. The possibility of using deep neural networks, Haar cascades, machine learning for mosquito recognition was considered. We considered in detail the classification problems of mosquitoes in images. A recommendation is given for the implementation of this device based on a microcontroller for subsequent use as part of an unmanned aerial vehicle. Any harmful insects in the fields can be used as objects for control.</div></div></div><div style="display:none;" class="absolute right-0 bottom-0"><span><span class="inline-block align-middle w-6 h-4 bg-gradient-to-r from-transparent to-white"></span><span class="bg-white">... </span></span><button class="m-button m-button--sm m-button--tertiary rounded !inline-flex after:hidden bg-white" type="button"><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center">Show more <svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="keyboard_arrow_down" style="" width="16" height="16" viewBox="0 0 16 16"></svg></span></button></div></div><div class="pt-sm text-color-subtle flex gap-x-sm gap-y-sm flex-col lg:flex-row" data-v-891fb557><p class="m-text text-sm" data-v-891fb557> Posted: 02 August 2021</p><p class="m-text text-sm underline cursor-pointer" data-v-891fb557><a href="/manuscript/202101.0412/v2" data-v-891fb557><span data-v-891fb557>https://doi.org/</span>10.20944/preprints202101.0412.v2</a></p></div></div><div data-v-891fb557></div></div><div data-v-891fb557><div class="border-b border-color-default pb-md mb-sm" data-v-891fb557><div class="pt-md lg:pt-lg lg:flex justify-between space-y-xs" data-v-891fb557><div class="flex flex-wrap gap-x-xs gap-y-xs" data-v-891fb557><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 bg-content-subtler text-color-default px-2 !bg-surface-disabled" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Review</span></div><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 border border-color-bold px-2 text-color-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Dietetics and Nutrition</span></div><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 border border-color-bold px-2 text-color-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Medicine and Pharmacology</span></div></div><div class="flex" data-v-891fb557><button class="m-button m-button--sm m-button--tertiary rounded mr-md after:hidden" type="button" data-v-891fb557><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="download" style="" width="16" height="16" viewBox="0 0 16 16" data-v-891fb557></svg> Download PDF </span></button><button class="m-button m-button--sm m-button--tertiary rounded after:hidden" type="button" data-v-891fb557><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="format_quote" style="" width="16" height="16" viewBox="0 0 16 16" data-v-891fb557></svg> Cite </span></button></div></div><a href="/manuscript/202003.0235/v2" class="" data-v-891fb557><h5 class="m-heading text-inherit m-h5 font-normal pt-sm hover:text-color-link-bold" data-v-891fb557>Evidence That Vitamin D Supplementation Could Reduce Risk of Influenza and COVID-19 Infections and Deaths</h5></a><div class="flex pt-sm flex-wrap gap-sm" data-v-891fb557><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>William B. Grant<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Henry Lahore<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Sharon L. McDonnell<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Carole A. Baggerly<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Christine B. French<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Jennifer L. Aliano<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Harjit Pal Bhattoa</p></div></div><div class="w-full relative overflow-hidden pt-sm" data-v-891fb557><div><div class="clamp-3"><div class="inline abstract-content" data-v-891fb557>Abstract: The world is in the grips of the COVID-19 pandemic. Public health measures that can reduce the risk of infection and death in addition to quarantines are desperately needed. This article reviews the roles of vitamin D in reducing risk of respiratory tract infections, knowledge about the epidemiology of influenza and COVID-19, and how vitamin D supplementation might be a useful measure to reduce risk. Through several mechanisms, vitamin D can reduce risk of infections. Those mechanisms include inducing cathelicidins and defensins that can lower viral replication rates and reducing concentrations of pro-inflammatory cytokines that produce the inflammation that injures the lining of the lungs, leading to pneumonia, as well as increase concentrations of anti-inflammatory cytokines. Several observational studies and clinical trials reported that vitamin D supplementation reduced risk of influenza, whereas others did not. Evidence supporting the role of vitamin D in reducing risk of COVID-19 includes that the outbreak occurred in winter, a time when 25-hydroxyvitamin D [25(OH)D] concentrations are lowest; that the number of cases in the Southern Hemisphere near the end of summer are low; that vitamin D deficiency has been found to contribute to acute respiratory distress syndrome, and that case-fatality rates increase with age and with chronic disease comorbidity, both of which are associated with lower 25(OH)D concentration. To reduce risk of infection, it is recommended that people at risk of influenza and/or COVID-19 consider taking 10,000 IU/d of vitamin D<sub>3</sub> for a few weeks to rapidly raise 25(OH)D concentrations, followed by 5000 IU/d. The goal should be to raise 25(OH)D concentrations above 40–60 ng/ml (100–150 nmol/l). For treatment of people who become infected with COVID-19, higher vitamin D<sub>3</sub> doses might be useful. Randomized controlled trials and large population studies should be conducted to evaluate these recommendations.</div></div><div class="invisible absolute"><div class="inline abstract-content" data-v-891fb557>Abstract: The world is in the grips of the COVID-19 pandemic. Public health measures that can reduce the risk of infection and death in addition to quarantines are desperately needed. This article reviews the roles of vitamin D in reducing risk of respiratory tract infections, knowledge about the epidemiology of influenza and COVID-19, and how vitamin D supplementation might be a useful measure to reduce risk. Through several mechanisms, vitamin D can reduce risk of infections. Those mechanisms include inducing cathelicidins and defensins that can lower viral replication rates and reducing concentrations of pro-inflammatory cytokines that produce the inflammation that injures the lining of the lungs, leading to pneumonia, as well as increase concentrations of anti-inflammatory cytokines. Several observational studies and clinical trials reported that vitamin D supplementation reduced risk of influenza, whereas others did not. Evidence supporting the role of vitamin D in reducing risk of COVID-19 includes that the outbreak occurred in winter, a time when 25-hydroxyvitamin D [25(OH)D] concentrations are lowest; that the number of cases in the Southern Hemisphere near the end of summer are low; that vitamin D deficiency has been found to contribute to acute respiratory distress syndrome, and that case-fatality rates increase with age and with chronic disease comorbidity, both of which are associated with lower 25(OH)D concentration. To reduce risk of infection, it is recommended that people at risk of influenza and/or COVID-19 consider taking 10,000 IU/d of vitamin D<sub>3</sub> for a few weeks to rapidly raise 25(OH)D concentrations, followed by 5000 IU/d. The goal should be to raise 25(OH)D concentrations above 40–60 ng/ml (100–150 nmol/l). For treatment of people who become infected with COVID-19, higher vitamin D<sub>3</sub> doses might be useful. Randomized controlled trials and large population studies should be conducted to evaluate these recommendations.</div></div></div><div style="display:none;" class="absolute right-0 bottom-0"><span><span class="inline-block align-middle w-6 h-4 bg-gradient-to-r from-transparent to-white"></span><span class="bg-white">... </span></span><button class="m-button m-button--sm m-button--tertiary rounded !inline-flex after:hidden bg-white" type="button"><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center">Show more <svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="keyboard_arrow_down" style="" width="16" height="16" viewBox="0 0 16 16"></svg></span></button></div></div><div class="pt-sm text-color-subtle flex gap-x-sm gap-y-sm flex-col lg:flex-row" data-v-891fb557><p class="m-text text-sm" data-v-891fb557> Posted: 30 March 2020</p><p class="m-text text-sm underline cursor-pointer" data-v-891fb557><a href="/manuscript/202003.0235/v2" data-v-891fb557><span data-v-891fb557>https://doi.org/</span>10.20944/preprints202003.0235.v2</a></p></div></div><div data-v-891fb557></div></div><div data-v-891fb557><div class="border-b border-color-default pb-md mb-sm" data-v-891fb557><div class="pt-md lg:pt-lg lg:flex justify-between space-y-xs" data-v-891fb557><div class="flex flex-wrap gap-x-xs gap-y-xs" data-v-891fb557><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 bg-content-subtler text-color-default px-2 !bg-surface-disabled" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Review</span></div><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 border border-color-bold px-2 text-color-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Transplantation</span></div><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 border border-color-bold px-2 text-color-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Medicine and Pharmacology</span></div></div><div class="flex" data-v-891fb557><button class="m-button m-button--sm m-button--tertiary rounded mr-md after:hidden" type="button" data-v-891fb557><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="download" style="" width="16" height="16" viewBox="0 0 16 16" data-v-891fb557></svg> Download PDF </span></button><button class="m-button m-button--sm m-button--tertiary rounded after:hidden" type="button" data-v-891fb557><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="format_quote" style="" width="16" height="16" viewBox="0 0 16 16" data-v-891fb557></svg> Cite </span></button></div></div><a href="/manuscript/202403.0881/v2" class="" data-v-891fb557><h5 class="m-heading text-inherit m-h5 font-normal pt-sm hover:text-color-link-bold" data-v-891fb557>Transfusions of Blood Products Derived from Genetic Vaccine Recipients: Safety Concerns and Proposals for Specific Measures</h5></a><div class="flex pt-sm flex-wrap gap-sm" data-v-891fb557><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Jun Ueda<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Hideyuki Motohashi<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Yuriko Hirai<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Kenji Yamamoto<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Yasufumi Murakami<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Masanori Fukushima<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Akinori Fujisawa</p></div></div><div class="w-full relative overflow-hidden pt-sm" data-v-891fb557><div><div class="clamp-3"><div class="inline abstract-content" data-v-891fb557>Abstract: <p>The World Health Organization declared the coronavirus disease 2019 (COVID-19) pandemic in 2020, following which a global genetic vaccination program has been rapidly implemented as a fundamental solution. However, it has been reported worldwide that the modified mRNAs encoding spike proteins and lipid nanoparticles, which are used as drug delivery systems, not only cause thrombosis and cardiovascular disorders post vaccination, but might also cause diverse diseases involving all organs and systems, including the nervous system. Furthermore, the toxicity and pathogenicity of spike proteins may necessitate defining these proteins as nonbiological infective material. Based on these reports and the abundant evidence that has come to light in the past few years, this paper aims to draw the attention of medical professionals to the various risks associated with transfusion using blood products derived from long COVID patients or from genetic vaccine recipients, and to make proposals regarding specific inspection items, testing methods, regulations, etc. This paper provides insights to address the post-vaccination syndrome and its consequences following such genetic vaccination programs.</p></div></div><div class="invisible absolute"><div class="inline abstract-content" data-v-891fb557>Abstract: <p>The World Health Organization declared the coronavirus disease 2019 (COVID-19) pandemic in 2020, following which a global genetic vaccination program has been rapidly implemented as a fundamental solution. However, it has been reported worldwide that the modified mRNAs encoding spike proteins and lipid nanoparticles, which are used as drug delivery systems, not only cause thrombosis and cardiovascular disorders post vaccination, but might also cause diverse diseases involving all organs and systems, including the nervous system. Furthermore, the toxicity and pathogenicity of spike proteins may necessitate defining these proteins as nonbiological infective material. Based on these reports and the abundant evidence that has come to light in the past few years, this paper aims to draw the attention of medical professionals to the various risks associated with transfusion using blood products derived from long COVID patients or from genetic vaccine recipients, and to make proposals regarding specific inspection items, testing methods, regulations, etc. This paper provides insights to address the post-vaccination syndrome and its consequences following such genetic vaccination programs.</p></div></div></div><div style="display:none;" class="absolute right-0 bottom-0"><span><span class="inline-block align-middle w-6 h-4 bg-gradient-to-r from-transparent to-white"></span><span class="bg-white">... </span></span><button class="m-button m-button--sm m-button--tertiary rounded !inline-flex after:hidden bg-white" type="button"><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center">Show more <svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="keyboard_arrow_down" style="" width="16" height="16" viewBox="0 0 16 16"></svg></span></button></div></div><div class="pt-sm text-color-subtle flex gap-x-sm gap-y-sm flex-col lg:flex-row" data-v-891fb557><p class="m-text text-sm" data-v-891fb557> Posted: 29 May 2024</p><p class="m-text text-sm underline cursor-pointer" data-v-891fb557><a href="/manuscript/202403.0881/v2" data-v-891fb557><span data-v-891fb557>https://doi.org/</span>10.20944/preprints202403.0881.v2</a></p></div></div><div data-v-891fb557></div></div><div data-v-891fb557><div class="border-b border-color-default pb-md mb-sm" data-v-891fb557><div class="pt-md lg:pt-lg lg:flex justify-between space-y-xs" data-v-891fb557><div class="flex flex-wrap gap-x-xs gap-y-xs" data-v-891fb557><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 bg-content-subtler text-color-default px-2 !bg-surface-disabled" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Article</span></div><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 border border-color-bold px-2 text-color-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Algebra and Number Theory</span></div><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 border border-color-bold px-2 text-color-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Computer Science and Mathematics</span></div></div><div class="flex" data-v-891fb557><button class="m-button m-button--sm m-button--tertiary rounded mr-md after:hidden" type="button" data-v-891fb557><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="download" style="" width="16" height="16" viewBox="0 0 16 16" data-v-891fb557></svg> Download PDF </span></button><button class="m-button m-button--sm m-button--tertiary rounded after:hidden" type="button" data-v-891fb557><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="format_quote" style="" width="16" height="16" viewBox="0 0 16 16" data-v-891fb557></svg> Cite </span></button></div></div><a href="/manuscript/202108.0146/v9" class="" data-v-891fb557><h5 class="m-heading text-inherit m-h5 font-normal pt-sm hover:text-color-link-bold" data-v-891fb557>A Proof of the Riemann Hypothesis Based on MacLaurin Expansion and Hadamard Product of the Completed Zeta Function</h5></a><div class="flex pt-sm flex-wrap gap-sm" data-v-891fb557><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Weicun Zhang</p></div></div><div class="w-full relative overflow-hidden pt-sm" data-v-891fb557><div><div class="clamp-3"><div class="inline abstract-content" data-v-891fb557>Abstract: The basic idea is to expand the completed zeta function $\xi(s)$ in MacLaurin series (infinite polynomial), which can be further expressed as infinite product (Hadamard product) by conjugate complex roots. Finally, the functional equation $\xi(s)=\xi(1-s)$ leads to $(s-\alpha_i)^2 = (1-s-\alpha_i)^2, i \in \mathbb{N}$ with solution $\alpha_i= \frac{1}{2}, i \in \mathbb{N}$, where $\alpha_i$ are the real parts of the zeros of $\xi(s)$, i.e., $s_i =\alpha_i\pm j\beta_i, i\in \mathbb{N}$. Therefore, a proof of the Riemann Hypothesis is achieved.</div></div><div class="invisible absolute"><div class="inline abstract-content" data-v-891fb557>Abstract: The basic idea is to expand the completed zeta function $\xi(s)$ in MacLaurin series (infinite polynomial), which can be further expressed as infinite product (Hadamard product) by conjugate complex roots. Finally, the functional equation $\xi(s)=\xi(1-s)$ leads to $(s-\alpha_i)^2 = (1-s-\alpha_i)^2, i \in \mathbb{N}$ with solution $\alpha_i= \frac{1}{2}, i \in \mathbb{N}$, where $\alpha_i$ are the real parts of the zeros of $\xi(s)$, i.e., $s_i =\alpha_i\pm j\beta_i, i\in \mathbb{N}$. Therefore, a proof of the Riemann Hypothesis is achieved.</div></div></div><div style="display:none;" class="absolute right-0 bottom-0"><span><span class="inline-block align-middle w-6 h-4 bg-gradient-to-r from-transparent to-white"></span><span class="bg-white">... </span></span><button class="m-button m-button--sm m-button--tertiary rounded !inline-flex after:hidden bg-white" type="button"><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center">Show more <svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="keyboard_arrow_down" style="" width="16" height="16" viewBox="0 0 16 16"></svg></span></button></div></div><div class="pt-sm text-color-subtle flex gap-x-sm gap-y-sm flex-col lg:flex-row" data-v-891fb557><p class="m-text text-sm" data-v-891fb557> Posted: 14 October 2021</p><p class="m-text text-sm underline cursor-pointer" data-v-891fb557><a href="/manuscript/202108.0146/v9" data-v-891fb557><span data-v-891fb557>https://doi.org/</span>10.20944/preprints202108.0146.v9</a></p></div></div><div data-v-891fb557></div></div><div data-v-891fb557><div class="border-b border-color-default pb-md mb-sm" data-v-891fb557><div class="pt-md lg:pt-lg lg:flex justify-between space-y-xs" data-v-891fb557><div class="flex flex-wrap gap-x-xs gap-y-xs" data-v-891fb557><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 bg-content-subtler text-color-default px-2 !bg-surface-disabled" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Article</span></div><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 border border-color-bold px-2 text-color-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Immunology and Microbiology</span></div><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 border border-color-bold px-2 text-color-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Biology and Life Sciences</span></div></div><div class="flex" data-v-891fb557><button class="m-button m-button--sm m-button--tertiary rounded mr-md after:hidden" type="button" data-v-891fb557><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="download" style="" width="16" height="16" viewBox="0 0 16 16" data-v-891fb557></svg> Download PDF </span></button><button class="m-button m-button--sm m-button--tertiary rounded after:hidden" type="button" data-v-891fb557><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="format_quote" style="" width="16" height="16" viewBox="0 0 16 16" data-v-891fb557></svg> Cite </span></button></div></div><a href="/manuscript/202002.0051/v3" class="" data-v-891fb557><h5 class="m-heading text-inherit m-h5 font-normal pt-sm hover:text-color-link-bold" data-v-891fb557>Bulk and Single-Cell Transcriptomics Identify Tobacco-Use Disparity in Lung Gene Expression of ACE2, the Receptor of 2019-nCov</h5></a><div class="flex pt-sm flex-wrap gap-sm" data-v-891fb557><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Guoshuai Cai</p></div></div><div class="w-full relative overflow-hidden pt-sm" data-v-891fb557><div><div class="clamp-3"><div class="inline abstract-content" data-v-891fb557>Abstract: In current severe global emergency situation of 2019-nCov outbreak, it is imperative to identify vulnerable and susceptible groups for effective protection and care. Recently, studies found that 2019-nCov and SARS-nCov share the same receptor, ACE2. In this study, we analyzed five large-scale bulk transcriptomic datasets of normal lung tissue and two single-cell transcriptomic datasets to investigate the disparities related to race, age, gender and smoking status in <em>ACE2</em> gene expression and its distribution among cell types. We didn’t find significant disparities in <em>ACE2</em> gene expression between racial groups (Asian vs Caucasian), age groups (>60 vs <60) or gender groups (male vs female). However, we observed significantly higher <em>ACE2</em> gene expression in former smoker’s lung compared to non-smoker’s lung. Also, we found higher <em>ACE2</em> gene expression in Asian current smokers compared to non-smokers but not in Caucasian current smokers, which may indicate an existence of gene-smoking interaction. In addition, we found that <em>ACE2</em> gene is expressed in specific cell types related to smoking history and location. In bronchial epithelium, <em>ACE2</em> is actively expressed in goblet cells of current smokers and club cells of non-smokers. In alveoli, <em>ACE2</em> is actively expressed in remodelled AT2 cells of former smokers. Together, this study indicates that smokers especially former smokers may be more susceptible to 2019-nCov and have infection paths different with non-smokers. Thus, smoking history may provide valuable information in identifying susceptible population and standardizing treatment regimen.</div></div><div class="invisible absolute"><div class="inline abstract-content" data-v-891fb557>Abstract: In current severe global emergency situation of 2019-nCov outbreak, it is imperative to identify vulnerable and susceptible groups for effective protection and care. Recently, studies found that 2019-nCov and SARS-nCov share the same receptor, ACE2. In this study, we analyzed five large-scale bulk transcriptomic datasets of normal lung tissue and two single-cell transcriptomic datasets to investigate the disparities related to race, age, gender and smoking status in <em>ACE2</em> gene expression and its distribution among cell types. We didn’t find significant disparities in <em>ACE2</em> gene expression between racial groups (Asian vs Caucasian), age groups (>60 vs <60) or gender groups (male vs female). However, we observed significantly higher <em>ACE2</em> gene expression in former smoker’s lung compared to non-smoker’s lung. Also, we found higher <em>ACE2</em> gene expression in Asian current smokers compared to non-smokers but not in Caucasian current smokers, which may indicate an existence of gene-smoking interaction. In addition, we found that <em>ACE2</em> gene is expressed in specific cell types related to smoking history and location. In bronchial epithelium, <em>ACE2</em> is actively expressed in goblet cells of current smokers and club cells of non-smokers. In alveoli, <em>ACE2</em> is actively expressed in remodelled AT2 cells of former smokers. Together, this study indicates that smokers especially former smokers may be more susceptible to 2019-nCov and have infection paths different with non-smokers. Thus, smoking history may provide valuable information in identifying susceptible population and standardizing treatment regimen.</div></div></div><div style="display:none;" class="absolute right-0 bottom-0"><span><span class="inline-block align-middle w-6 h-4 bg-gradient-to-r from-transparent to-white"></span><span class="bg-white">... </span></span><button class="m-button m-button--sm m-button--tertiary rounded !inline-flex after:hidden bg-white" type="button"><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center">Show more <svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="keyboard_arrow_down" style="" width="16" height="16" viewBox="0 0 16 16"></svg></span></button></div></div><div class="pt-sm text-color-subtle flex gap-x-sm gap-y-sm flex-col lg:flex-row" data-v-891fb557><p class="m-text text-sm" data-v-891fb557> Posted: 02 March 2020</p><p class="m-text text-sm underline cursor-pointer" data-v-891fb557><a href="/manuscript/202002.0051/v3" data-v-891fb557><span data-v-891fb557>https://doi.org/</span>10.20944/preprints202002.0051.v3</a></p></div></div><div data-v-891fb557></div></div><div data-v-891fb557><div class="border-b border-color-default pb-md mb-sm" data-v-891fb557><div class="pt-md lg:pt-lg lg:flex justify-between space-y-xs" data-v-891fb557><div class="flex flex-wrap gap-x-xs gap-y-xs" data-v-891fb557><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 bg-content-subtler text-color-default px-2 !bg-surface-disabled" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Article</span></div><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 border border-color-bold px-2 text-color-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Epidemiology and Infectious Diseases</span></div><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 border border-color-bold px-2 text-color-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">Medicine and Pharmacology</span></div></div><div class="flex" data-v-891fb557><button class="m-button m-button--sm m-button--tertiary rounded mr-md after:hidden" type="button" data-v-891fb557><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="download" style="" width="16" height="16" viewBox="0 0 16 16" data-v-891fb557></svg> Download PDF </span></button><button class="m-button m-button--sm m-button--tertiary rounded after:hidden" type="button" data-v-891fb557><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="format_quote" style="" width="16" height="16" viewBox="0 0 16 16" data-v-891fb557></svg> Cite </span></button></div></div><a href="/manuscript/202005.0057/v2" class="" data-v-891fb557><h5 class="m-heading text-inherit m-h5 font-normal pt-sm hover:text-color-link-bold" data-v-891fb557>Early Hydroxychloroquine Is Associated with an Increase of Survival in COVID-19 Patients: An Observational Study</h5></a><div class="flex pt-sm flex-wrap gap-sm" data-v-891fb557><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Francisco Javier Membrillo<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Germán Ramírez-Olivencia<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Miriam Estébanez<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Begoña de Dios<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>María Dolores Herrero<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Tatiana Mata<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Alberto M. Borobia<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Carlos Gutiérrez<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>María Simón<span data-v-891fb557>,</span></p></div><div class="flex items-center" data-v-891fb557><p class="m-text text-sm text-color-subtlest" data-v-891fb557>Ana Ochoa</p></div><div data-v-891fb557><div class="m-tag flex items-center gap-2 rounded py-0.5 h-6 bg-content-subtler text-color-default px-2 bg-info-subtlest" data-v-891fb557><span class="flex items-center whitespace-nowrap text-xs leading-normal">+9 authors</span></div></div></div><div class="w-full relative overflow-hidden pt-sm" data-v-891fb557><div><div class="clamp-3"><div class="inline abstract-content" data-v-891fb557>Abstract: <em>Background:</em> Although no specific treatment for COVID 19 has been proven effective yet, some drugs with in vitro potential against SARS-CoV-2 virus have been proposed for clinical use. Hydroxychloroquine has in vitro anti-viral and immunomodulatory activity, but there is no current clinical evidence of its effectiveness on the outcome of the disease. <em>Methods:</em> We enrolled all 18-85 years old inpatients from Central Defense Hospital, Madrid, Spain, who were hospitalised due to COVID-19 and had a definitive outcome (either dead or discharged). We used a statistical survival analysis. <em>Results:</em> We analysed 220 medical records. 166 patients met the inclusion criteria. 48,8 % of patients not treated with HCQ died, versus 22% in the group of hydroxychloroquine (p=0,002). According to clinical picture at admission, hydroxychloroquine increased the mean cumulative survival in all groups from 1,4 to 1,8 times. This difference was statistically significant in the mild group. <em>Conclusions:</em> in a cohort of 166 patients between 18 to 85 years hospitalised with COVID-19, hydroxychloroquine treatment with an initial loading dose of 800mg improved patient survival when admitted in early stages of the disease. There was a non-statistically significant trend towards survival in all groups, which will need to be clarified in subsequent studies.</div></div><div class="invisible absolute"><div class="inline abstract-content" data-v-891fb557>Abstract: <em>Background:</em> Although no specific treatment for COVID 19 has been proven effective yet, some drugs with in vitro potential against SARS-CoV-2 virus have been proposed for clinical use. Hydroxychloroquine has in vitro anti-viral and immunomodulatory activity, but there is no current clinical evidence of its effectiveness on the outcome of the disease. <em>Methods:</em> We enrolled all 18-85 years old inpatients from Central Defense Hospital, Madrid, Spain, who were hospitalised due to COVID-19 and had a definitive outcome (either dead or discharged). We used a statistical survival analysis. <em>Results:</em> We analysed 220 medical records. 166 patients met the inclusion criteria. 48,8 % of patients not treated with HCQ died, versus 22% in the group of hydroxychloroquine (p=0,002). According to clinical picture at admission, hydroxychloroquine increased the mean cumulative survival in all groups from 1,4 to 1,8 times. This difference was statistically significant in the mild group. <em>Conclusions:</em> in a cohort of 166 patients between 18 to 85 years hospitalised with COVID-19, hydroxychloroquine treatment with an initial loading dose of 800mg improved patient survival when admitted in early stages of the disease. There was a non-statistically significant trend towards survival in all groups, which will need to be clarified in subsequent studies.</div></div></div><div style="display:none;" class="absolute right-0 bottom-0"><span><span class="inline-block align-middle w-6 h-4 bg-gradient-to-r from-transparent to-white"></span><span class="bg-white">... </span></span><button class="m-button m-button--sm m-button--tertiary rounded !inline-flex after:hidden bg-white" type="button"><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center">Show more <svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="keyboard_arrow_down" style="" width="16" height="16" viewBox="0 0 16 16"></svg></span></button></div></div><div class="pt-sm text-color-subtle flex gap-x-sm gap-y-sm flex-col lg:flex-row" data-v-891fb557><p class="m-text text-sm" data-v-891fb557> Posted: 09 May 2020</p><p class="m-text text-sm underline cursor-pointer" data-v-891fb557><a href="/manuscript/202005.0057/v2" data-v-891fb557><span data-v-891fb557>https://doi.org/</span>10.20944/preprints202005.0057.v2</a></p></div></div><div data-v-891fb557></div></div><div class="m-pagination flex items-center gap-md pt-lg"><button disabled class="m-button m-button--lg m-button--secondary m-button--disabled rounded !pl-sm" type="button" data-testid="prev-button"><span class="inline-flex h-full w-full items-center gap-2 whitespace-nowrap justify-center"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" data-testid="chevron_left" style="" width="24" height="24" viewBox="0 0 16 16"></svg><span>Prev</span><span class="sr-only">Go to previous page</span></span></button><div class="m-input common-field m-input--filled max-w-[5rem] [&_input]:text-center"><div class="common-field__wrapper m-input__label"><div class="common-field__input flex items-center rounded h-10"><input 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Explore the latest trends in healthcare that impact population well-being and equity.","public-health-and-healthcare",{"id":117,"name":118,"name_system":119,"parent_id":50,"image_banner":120,"description":121,"converted_name_system":122},193,"Social Sciences","social_sciences","https://www.preprints.org/media/cache/resolve/webp/upload/subject/2024-09-12/910c960e3a6808861523728f0f26b2df.png","Gain insights into human behavior, societies, and cultures with our preprints. Explore disciplines like psychology, sociology, and political science, which shape our world and inform public policy. Stay engaged with research that explores social issues, cultural dynamics, and economic systems, enriching our understanding of human interactions.","social-sciences",{"code":41,"msg":124,"data":125},"",{"total":126,"rows":127,"filters":498},76577,[128,204,228,260,292,312,349,386,405,425],{"views":129,"true_downloads":130,"true_views":131,"downloads":132,"id":133,"version":134,"hash_key":135,"article_type":136,"subject":97,"top_subject":137,"title":138,"abstract":139,"keywords":140,"doi":141,"published_at":142,"download_url":143,"liked":144,"like_url":145,"authors":146,"score":7},452912,2847,9885,102365,"202004.0203",4,"b56ce854948f943ea12c645e87386067","Review","Immunology and Allergy","Face Masks Against COVID-19: An Evidence Review","The science around the use of masks by the general public to impede COVID-19 transmission is advancing rapidly. Policymakers need guidance on how masks should be used by the general population to combat the COVID-19 pandemic. In this narrative review, we develop an analytical framework to examine mask usage, considering and synthesizing the relevant literature to inform multiple areas: population impact; transmission characteristics; source control; PPE; sociological considerations; and implementation considerations. A primary route of transmission of COVID-19 is via respiratory droplets, and is known to be transmissible from presymptomatic and asymptomatic individuals. Reducing disease spread requires two things: first, limit contacts of infected individuals via physical distancing and other measures, and second, reduce the transmission probability per contact. The preponderance of evidence indicates that mask wearing reduces the transmissibility per contact by reducing transmission of infected droplets in both laboratory and clinical contexts. Public mask wearing is most effective at reducing spread of the virus when compliance is high. The decreased transmissibility could substantially reduce the death toll and economic impact while the cost of the intervention is low. Given the current shortages of medical masks we recommend the adoption of public cloth mask wearing, as an effective form of source control, in conjunction with existing hygiene, distancing, and contact tracing strategies. Because many respiratory droplets become smaller due to evaporation, we recommend increasing focus on a previously overlooked aspect of mask usage: mask-wearing by infectious people (\"source control\") with benefits at the population-level, rather than mask-wearing by susceptible people, such as health-care workers, with focus on individual outcomes. We recommend that public officials and governments strongly encourage the use of widespread face masks in public, including the use of appropriate regulation.","COVID-19; SARS-CoV-2; masks; pandemic","10.20944/preprints202004.0203.v4","2020-11-02T09:18:00Z","/frontend/manuscript/b56ce854948f943ea12c645e87386067/download_pub",false,"/frontend/user/manuscript/b56ce854948f943ea12c645e87386067/like_toggle",[147,150,153,156,159,162,165,168,171,174,177,180,183,186,189,192,195,198,201],{"name":148,"email":149},"Jeremy Howard","j@fast.ai",{"name":151,"email":152},"Austin Huang","austinvh@gmail.com",{"name":154,"email":155},"Zhiyuan Li","zhiyuanli@pku.edu.cn",{"name":157,"email":158},"Zeynep Tufekci","zeynep@technosociology.org",{"name":160,"email":161},"Vladimir Zdimal","Zdimal@icpf.cas.cz",{"name":163,"email":164},"Helene-Mari van der Westhuizen","helene1mari@gmail.com",{"name":166,"email":167},"Arne von Delft","vuzumsi@gmail.com",{"name":169,"email":170},"Amy Price","amyprice@stanford.edu",{"name":172,"email":173},"Lex Fridman","fridman@mit.edu",{"name":175,"email":176},"Lei-Han Tang","lhtang@hkbu.edu.hk",{"name":178,"email":179},"Viola Tang","viola.jx.tang@gmail.com",{"name":181,"email":182},"Gregory L. Watson","gwatson@ucla.edu",{"name":184,"email":185},"Christina E. Bax","Christina.Bax@pennmedicine.upenn.edu",{"name":187,"email":188},"Reshama Shaikh","reshama.shaikh@gmail.com",{"name":190,"email":191},"Frederik Questier","fquestie@vub.ac.be",{"name":193,"email":194},"Danny Hernandez","danny@openai.com",{"name":196,"email":197},"Larry F. Chu","lchu@stanford.edu",{"name":199,"email":200},"Christina M. Ramirez","cr@ucla.edu",{"name":202,"email":203},"Anne W. Rimoin","arimoin@g.ucla.edu",{"views":205,"true_downloads":206,"true_views":205,"downloads":206,"id":207,"version":7,"hash_key":208,"article_type":209,"subject":97,"top_subject":210,"title":211,"abstract":212,"keywords":213,"doi":214,"published_at":215,"download_url":216,"liked":144,"like_url":217,"authors":218,"score":7},308766,42111,"202007.0025","2b8171df5f247daef6bc53f7dc9457c4","Article","Pharmacy","COVID-19 Outpatients – Early Risk-Stratified Treatment with Zinc Plus Low Dose Hydroxychloroquine and Azithromycin: A Retrospective Case Series Study","\u003Cem>Objective: \u003C/em>To describe outcomes of patients with coronavirus disease 2019 (COVID-19) in the outpatient setting after early treatment with zinc, low dose hydroxychloroquine, and azithromycin (the triple therapy) dependent on risk stratification. \u003Cem>Design:\u003C/em> Retrospective case series study. \u003Cem>Setting:\u003C/em> General practice. \u003Cem>Participants:\u003C/em> 141 COVID-19 patients with laboratory confirmed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections in the year 2020. \u003Cem>Main Outcome Measures:\u003C/em> Risk-stratified treatment decision, rate of hospitalization and all-cause death. \u003Cem>Results: \u003C/em>Of 335 positively PCR-tested COVID-19 patients, 127 were treated with the triple therapy. 104 of 127 met the defined risk stratification criteria and were included in the analysis. In addition, 37 treated and eligible patients who were confirmed by IgG tests were included in the treatment group (total N=141). 208 of the 335 patients did not meet the risk stratification criteria and were not treated. After 4 days (median, IQR 3-6, available for N=66/141) of onset of symptoms, 141 patients (median age 58 years, IQR 40-67; 73% male) got a prescription for the triple therapy for 5 days. Independent public reference data from 377 confirmed COVID-19 patients of the same community were used as untreated control. 4 of 141 treated patients (2.8%) were hospitalized, which was significantly less (p<0.001) compared with 58 of 377 untreated patients (15.4%) (odds ratio 0.16, 95% CI 0.06-0.5). Therefore, the odds of hospitalization of treated patients were 84% less than in the untreated group. One patient (0.7%) died in the treatment group versus 13 patients (3.5%) in the untreated group (odds ratio 0.2, 95% CI 0.03-1.5; p=0.16). There were no cardiac side effects. \u003Cem>Conclusions:\u003C/em> Risk stratification-based treatment of COVID-19 outpatients as early as possible after symptom onset with the used triple therapy, including the combination of zinc with low dose hydroxychloroquine, was associated with significantly less hospitalizations and 5 times less all-cause deaths.","SARS-CoV-2; COVID-19; outpatients; treatment; zinc; hydroxychloroquine; azithromycin","10.20944/preprints202007.0025.v1","2020-07-03T06:52:22Z","/frontend/manuscript/2b8171df5f247daef6bc53f7dc9457c4/download_pub","/frontend/user/manuscript/2b8171df5f247daef6bc53f7dc9457c4/like_toggle",[219,222,225],{"name":220,"email":221},"Martin Scholz","martin.scholz@leukocare.com",{"name":223,"email":224},"Roland Derwand","roland.derwand@gmx.de",{"name":226,"email":227},"Vladimir Zelenko","zz613@hotmail.com",{"views":229,"true_downloads":230,"true_views":229,"downloads":230,"id":231,"version":7,"hash_key":232,"article_type":209,"subject":56,"top_subject":233,"title":234,"abstract":235,"keywords":236,"doi":237,"published_at":238,"download_url":239,"liked":144,"like_url":240,"authors":241,"score":7},215757,33792,"202004.0315","29e56f22d80f1b6d45ef7aefab4783cc","Biochemistry and Molecular Biology","In Search of Preventative Strategies: Novel Anti-Inflammatory High-CBD \u003Cem>Cannabis Sativa\u003C/em> Extracts Modulate ACE2 Expression in COVID-19 Gateway Tissues","With the rapidly growing pandemic of COVID-19 caused by the new and challenging to treat zoonotic SARS-CoV2 coronavirus, there is an urgent need for new therapies and prevention strategies that can help curtail disease spread and reduce mortality. Inhibition of viral entry and thereby spread constitute plausible therapeutic avenues. Similar to other respiratory pathogens, SARS-CoV2 is transmitted through respiratory droplets, with potential for aerosol and contact spread. It uses receptor-mediated entry into the human host via angiotensin-converting enzyme II (ACE2) that is expressed in lung tissue, as well as oral and nasal mucosa, kidney, testes, and the gastrointestinal tract. Modulation of ACE2 levels in these gateway tissues may prove a plausible strategy for decreasing disease susceptibility. Cannabis sativa, especially one high in the anti-inflammatory cannabinoid cannabidiol (CBD), has been proposed to modulate gene expression and inflammation and harbour anti-cancer and anti-inflammatory properties. Working under the Health Canada research license, we have developed over 800 new Cannabis sativa lines and extracts and hypothesized that high-CBD C. sativa extracts may be used to modulate ACE2 expression in COVID-19 target tissues. Screening C. sativa extracts using artificial human 3D models of oral, airway, and intestinal tissues, we identified 13 high CBD C. sativa extracts that modulate ACE2 gene expression and ACE2 protein levels. Our initial data suggest that some C. sativa extract down-regulate serine protease TMPRSS2, another critical protein required for SARS-CoV2 entry into host cells. While our most effective extracts require further large-scale validation, our study is crucial for the future analysis of the effects of medical cannabis on COVID-19. The extracts of our most successful and novel high CBD C. sativa lines, pending further investigation, may become a useful and safe addition to the treatment of COVID-19 as an adjunct therapy. They can be used to develop easy-to-use preventative treatments in the form of mouthwash and throat gargle products for both clinical and at-home use. Such products ought to be tested for their potential to decrease viral entry via the oral mucosa. Given the current dire and rapidly evolving epidemiological situation, every possible therapeutic opportunity and avenue must be considered.","COVID-19; SARS-CoV2; ACE2 receptor; medical cannabis; CBD","10.20944/preprints202004.0315.v1","2020-04-19T00:45:50Z","/frontend/manuscript/29e56f22d80f1b6d45ef7aefab4783cc/download_pub","/frontend/user/manuscript/29e56f22d80f1b6d45ef7aefab4783cc/like_toggle",[242,245,248,251,254,257],{"name":243,"email":244},"Bo Wang","bo.wang5@uleth.ca",{"name":246,"email":247},"Anna Kovalchuk","anna.kovalchuk1@ucalgary.ca",{"name":249,"email":250},"Dongping Li","dongping.li@uleth.ca",{"name":252,"email":253},"Yaroslav Ilnytskyy","slava.ilyntskyy@uleth.ca",{"name":255,"email":256},"Igor Kovalchuk","igor.kovalchuk@uleth.ca",{"name":258,"email":259},"Olga Kovalchuk","olga.kovalchuk@uleth.ca",{"views":261,"true_downloads":262,"true_views":261,"downloads":262,"id":263,"version":7,"hash_key":264,"article_type":209,"subject":97,"top_subject":265,"title":266,"abstract":267,"keywords":268,"doi":269,"published_at":270,"download_url":271,"liked":144,"like_url":272,"authors":273,"score":7},148166,16758,"202208.0151","cda6cde90415d65a21383e7a1a56ec38","Cardiac and Cardiovascular Systems","Cardiovascular Effects of the BNT162b2 mRNA COVID-19 Vaccine in Adolescents","This study focuses on cardiovascular effects, particularly myocarditis and pericarditis events, after BNT162b2 mRNA COVID-19 vaccine injection in Thai adolescents. This prospective cohort study enrolled students from two schools aged 13–18 years who received the second dose of the BNT162b2 mRNA COVID-19 vaccine. Data including demographics, symptoms, vital signs, ECG, echocardiography and cardiac enzymes were collected at baseline, Day 3, Day 7, and Day 14 (optional) using case record forms.We enrolled 314 participants; of these, 13 participants were lost to follow up, leaving 301 participants for analysis. The most common cardiovascular effects were tachycardia (7.64%), shortness of breath (6.64%), palpitation (4.32%), chest pain (4.32%), and hypertension (3.99%). Seven participants (2.33%) exhibited at least one elevated cardiac biomarker or positive lab assessments. Cardiovascular effects were found in 29.24% of patients, ranging from tachycardia, palpitation, and myopericarditis. Myopericarditis was confirmed in one patient after vaccination. Two patients had suspected pericarditis and four patients had suspected subclinical myocarditis. Conclusion: Cardiovascular effects in adolescents after BNT162b2 mRNA COVID-19 vaccination included tachycardia, palpitation, and myocarditis. The clinical presentation of myopericarditis after vaccination was usually mild, with all cases fully recovering within 14 days. Hence, adolescents receiving mRNA vaccines should be monitored for side effects. Clinical Trial Registration: NCT05288231","BNT162b2 mRNA COVID-19 vaccine; COVID-19 vaccine; cardiovascular effects; myocarditis; adolescents; Thailand","10.20944/preprints202208.0151.v1","2022-08-08T08:40:23Z","/frontend/manuscript/cda6cde90415d65a21383e7a1a56ec38/download_pub","/frontend/user/manuscript/cda6cde90415d65a21383e7a1a56ec38/like_toggle",[274,277,280,283,286,289],{"name":275,"email":276},"Suyanee Mansanguan","nuth-snoopy@hotmail.com",{"name":278,"email":279},"Prakaykaew Charunwatthana","prakaykaew.cha@mahidol.ac.th",{"name":281,"email":282},"Watcharapong Piyaphanee","watcharapong.piy@mahidol.ac.th",{"name":284,"email":285},"Wilanee Dechkhajorn","wilanee.dec@mahidol.ac.th",{"name":287,"email":288},"Akkapon Poolcharoen","apoolcharoen@gmail.com",{"name":290,"email":291},"Chayasin Mansanguan","chayasin.man@mahidol.ac.th",{"views":293,"true_downloads":294,"true_views":295,"downloads":296,"id":297,"version":298,"hash_key":299,"article_type":209,"subject":56,"top_subject":300,"title":301,"abstract":302,"keywords":303,"doi":304,"published_at":305,"download_url":306,"liked":144,"like_url":307,"authors":308,"score":7},127948,588,1560,33176,"202101.0412",2,"0db81b764127a40593a510f0412550ce","Insect Science","Raspberry PI for Kill Mosquitoes by Laser","More than 700 thousand human deaths from mosquito bites are observed annually in the world. It is more than 2 times the number of annual murders in the world. In this regard, the invention of new more effective methods of protection against mosquitoes is necessary. In this article for the first time, comprehensive studies of mosquito neutralization using machine vision and a 1 W power laser are considered. Developed laser installation with Raspberry Pi that changing the direction of the laser with a galvanometer. We developed a program for mosquito tracking in real. The possibility of using deep neural networks, Haar cascades, machine learning for mosquito recognition was considered. We considered in detail the classification problems of mosquitoes in images. A recommendation is given for the implementation of this device based on a microcontroller for subsequent use as part of an unmanned aerial vehicle. Any harmful insects in the fields can be used as objects for control.","laser for mosquito control; deep learning for mosquito control; insect detection; mosquito neutralization; pest detection; insect recognition; Raspberry Pi3; Raspberry Pi4; OpenCV for small object detect","10.20944/preprints202101.0412.v2","2021-08-02T14:44:07Z","/frontend/manuscript/0db81b764127a40593a510f0412550ce/download_pub","/frontend/user/manuscript/0db81b764127a40593a510f0412550ce/like_toggle",[309],{"name":310,"email":311},"Ildar Rakhmatulin","ildar.o2010@yandex.ru",{"views":313,"true_downloads":314,"true_views":315,"downloads":316,"id":317,"version":298,"hash_key":318,"article_type":136,"subject":97,"top_subject":319,"title":320,"abstract":321,"keywords":322,"doi":323,"published_at":324,"download_url":325,"liked":144,"like_url":326,"authors":327,"score":7},113277,4354,25347,16383,"202003.0235","e13e89c0467f638e9ed43580d23bb7af","Dietetics and Nutrition","Evidence That Vitamin D Supplementation Could Reduce Risk of Influenza and COVID-19 Infections and Deaths","The world is in the grips of the COVID-19 pandemic. Public health measures that can reduce the risk of infection and death in addition to quarantines are desperately needed. This article reviews the roles of vitamin D in reducing risk of respiratory tract infections, knowledge about the epidemiology of influenza and COVID-19, and how vitamin D supplementation might be a useful measure to reduce risk. Through several mechanisms, vitamin D can reduce risk of infections. Those mechanisms include inducing cathelicidins and defensins that can lower viral replication rates and reducing concentrations of pro-inflammatory cytokines that produce the inflammation that injures the lining of the lungs, leading to pneumonia, as well as increase concentrations of anti-inflammatory cytokines. Several observational studies and clinical trials reported that vitamin D supplementation reduced risk of influenza, whereas others did not. Evidence supporting the role of vitamin D in reducing risk of COVID-19 includes that the outbreak occurred in winter, a time when 25-hydroxyvitamin D [25(OH)D] concentrations are lowest; that the number of cases in the Southern Hemisphere near the end of summer are low; that vitamin D deficiency has been found to contribute to acute respiratory distress syndrome, and that case-fatality rates increase with age and with chronic disease comorbidity, both of which are associated with lower 25(OH)D concentration. To reduce risk of infection, it is recommended that people at risk of influenza and/or COVID-19 consider taking 10,000 IU/d of vitamin D\u003Csub>3\u003C/sub> for a few weeks to rapidly raise 25(OH)D concentrations, followed by 5000 IU/d. The goal should be to raise 25(OH)D concentrations above 40–60 ng/ml (100–150 nmol/l). For treatment of people who become infected with COVID-19, higher vitamin D\u003Csub>3\u003C/sub> doses might be useful. Randomized controlled trials and large population studies should be conducted to evaluate these recommendations.","acute respiratory distress syndrome (ARDS); ascorbic acid; cathelicidin; coronavirus; COVID-19; cytokine storm; influenza; observational; pneumonia, prevention; respiratory tract infection; solar radiation; treatment; UVB; vitamin C; vitamin D","10.20944/preprints202003.0235.v2","2020-03-30T03:48:43Z","/frontend/manuscript/e13e89c0467f638e9ed43580d23bb7af/download_pub","/frontend/user/manuscript/e13e89c0467f638e9ed43580d23bb7af/like_toggle",[328,331,334,337,340,343,346],{"name":329,"email":330},"William B. Grant","wbgrant@infionline.net",{"name":332,"email":333},"Henry Lahore","hlahore@vitamindwiki.com",{"name":335,"email":336},"Sharon L. McDonnell","sharon@grassrootshealth.org",{"name":338,"email":339},"Carole A. Baggerly","carole@grassrootshealth.org",{"name":341,"email":342},"Christine B. French","christine@grassrootshealth.org",{"name":344,"email":345},"Jennifer L. Aliano","jen@grassrootshealth.org",{"name":347,"email":348},"Harjit Pal Bhattoa","harjit@med.unideb.hu",{"views":350,"true_downloads":351,"true_views":352,"downloads":353,"id":354,"version":298,"hash_key":355,"article_type":136,"subject":97,"top_subject":356,"title":357,"abstract":358,"keywords":359,"doi":360,"published_at":361,"download_url":362,"liked":144,"like_url":363,"authors":364,"score":7},87472,2203,9533,19133,"202403.0881","37da1875eaf1141b1336b5d3126236b4","Transplantation","Transfusions of Blood Products Derived from Genetic Vaccine Recipients: Safety Concerns and Proposals for Specific Measures","\u003Cp>The World Health Organization declared the coronavirus disease 2019 (COVID-19) pandemic in 2020, following which a global genetic vaccination program has been rapidly implemented as a fundamental solution. However, it has been reported worldwide that the modified mRNAs encoding spike proteins and lipid nanoparticles, which are used as drug delivery systems, not only cause thrombosis and cardiovascular disorders post vaccination, but might also cause diverse diseases involving all organs and systems, including the nervous system. Furthermore, the toxicity and pathogenicity of spike proteins may necessitate defining these proteins as nonbiological infective material. Based on these reports and the abundant evidence that has come to light in the past few years, this paper aims to draw the attention of medical professionals to the various risks associated with transfusion using blood products derived from long COVID patients or from genetic vaccine recipients, and to make proposals regarding specific inspection items, testing methods, regulations, etc. This paper provides insights to address the post-vaccination syndrome and its consequences following such genetic vaccination programs.\u003C/p>","COVID-19 vaccine; genetic vaccine; blood product; blood transfusion; spike protein; post-vaccination syndrome; harm–benefit assessment; prion; spikeopathy; inspection standard; diagnostic criteria ","10.20944/preprints202403.0881.v2","2024-05-29T06:33:19Z","/frontend/manuscript/37da1875eaf1141b1336b5d3126236b4/download_pub","/frontend/user/manuscript/37da1875eaf1141b1336b5d3126236b4/like_toggle",[365,368,371,374,377,380,383],{"name":366,"email":367},"Jun Ueda","junueda@asahikawa-med.ac.jp",{"name":369,"email":370},"Hideyuki Motohashi","moto@tokyo-med.ac.jp",{"name":372,"email":373},"Yuriko Hirai","hirai@mcl-corp.jp",{"name":375,"email":376},"Kenji Yamamoto","yamamoto@okamura.or.jp",{"name":378,"email":379},"Yasufumi Murakami","yasufumi@rs.tus.ac.jp",{"name":381,"email":382},"Masanori Fukushima","mfukushima@imrd.jp",{"name":384,"email":385},"Akinori Fujisawa","fujisawa.peace@mac.com",{"views":387,"true_downloads":388,"true_views":389,"downloads":390,"id":391,"version":5,"hash_key":392,"article_type":209,"subject":77,"top_subject":393,"title":394,"abstract":395,"keywords":396,"doi":397,"published_at":398,"download_url":399,"liked":144,"like_url":400,"authors":401,"score":7},70324,313,1708,8115,"202108.0146","e01b28fb7cb770c331968f60d170b3b4","Algebra and Number Theory","A Proof of the Riemann Hypothesis Based on MacLaurin Expansion and Hadamard Product of the Completed Zeta Function","The basic idea is to expand the completed zeta function $\\xi(s)$ in MacLaurin series (infinite polynomial), which can be further expressed as infinite product (Hadamard product) by conjugate complex roots. Finally, the functional equation $\\xi(s)=\\xi(1-s)$ leads to $(s-\\alpha_i)^2 = (1-s-\\alpha_i)^2, i \\in \\mathbb{N}$ with solution $\\alpha_i= \\frac{1}{2}, i \\in \\mathbb{N}$, where $\\alpha_i$ are the real parts of the zeros of $\\xi(s)$, i.e., $s_i =\\alpha_i\\pm j\\beta_i, i\\in \\mathbb{N}$. Therefore, a proof of the Riemann Hypothesis is achieved.","Riemann Hypothesis (RH); Proof ; Completed zeta function $\\xi(s)$","10.20944/preprints202108.0146.v9","2021-10-14T12:14:19Z","/frontend/manuscript/e01b28fb7cb770c331968f60d170b3b4/download_pub","/frontend/user/manuscript/e01b28fb7cb770c331968f60d170b3b4/like_toggle",[402],{"name":403,"email":404},"Weicun Zhang","weicunzhang@ustb.edu.cn",{"views":406,"true_downloads":407,"true_views":408,"downloads":409,"id":410,"version":411,"hash_key":412,"article_type":209,"subject":56,"top_subject":413,"title":414,"abstract":415,"keywords":416,"doi":417,"published_at":418,"download_url":419,"liked":144,"like_url":420,"authors":421,"score":7},66348,1135,5004,26825,"202002.0051",3,"d81822a9e0108e68e02b3872aee1ad7f","Immunology and Microbiology","Bulk and Single-Cell Transcriptomics Identify Tobacco-Use Disparity in Lung Gene Expression of ACE2, the Receptor of 2019-nCov","In current severe global emergency situation of 2019-nCov outbreak, it is imperative to identify vulnerable and susceptible groups for effective protection and care. Recently, studies found that 2019-nCov and SARS-nCov share the same receptor, ACE2. In this study, we analyzed five large-scale bulk transcriptomic datasets of normal lung tissue and two single-cell transcriptomic datasets to investigate the disparities related to race, age, gender and smoking status in \u003Cem>ACE2\u003C/em> gene expression and its distribution among cell types. We didn’t find significant disparities in \u003Cem>ACE2\u003C/em> gene expression between racial groups (Asian vs Caucasian), age groups (>60 vs <60) or gender groups (male vs female). However, we observed significantly higher \u003Cem>ACE2\u003C/em> gene expression in former smoker’s lung compared to non-smoker’s lung. Also, we found higher \u003Cem>ACE2\u003C/em> gene expression in Asian current smokers compared to non-smokers but not in Caucasian current smokers, which may indicate an existence of gene-smoking interaction. In addition, we found that \u003Cem>ACE2\u003C/em> gene is expressed in specific cell types related to smoking history and location. In bronchial epithelium, \u003Cem>ACE2\u003C/em> is actively expressed in goblet cells of current smokers and club cells of non-smokers. In alveoli, \u003Cem>ACE2\u003C/em> is actively expressed in remodelled AT2 cells of former smokers. Together, this study indicates that smokers especially former smokers may be more susceptible to 2019-nCov and have infection paths different with non-smokers. Thus, smoking history may provide valuable information in identifying susceptible population and standardizing treatment regimen.","Wuhan 2019-nCov; ACE2; expression; susceptibility; race; age; gender; smoking; single cell","10.20944/preprints202002.0051.v3","2020-03-02T00:38:52Z","/frontend/manuscript/d81822a9e0108e68e02b3872aee1ad7f/download_pub","/frontend/user/manuscript/d81822a9e0108e68e02b3872aee1ad7f/like_toggle",[422],{"name":423,"email":424},"Guoshuai Cai","caigs.whu@gmail.com",{"views":426,"true_downloads":427,"true_views":428,"downloads":429,"id":430,"version":298,"hash_key":431,"article_type":209,"subject":97,"top_subject":432,"title":433,"abstract":434,"keywords":435,"doi":436,"published_at":437,"download_url":438,"liked":144,"like_url":439,"authors":440,"score":7},63265,2389,11048,10187,"202005.0057","e6fa45c47a059a99319d068c163554c2","Epidemiology and Infectious Diseases","Early Hydroxychloroquine Is Associated with an Increase of Survival in COVID-19 Patients: An Observational Study","\u003Cem>Background:\u003C/em> Although no specific treatment for COVID 19 has been proven effective yet, some drugs with in vitro potential against SARS-CoV-2 virus have been proposed for clinical use. Hydroxychloroquine has in vitro anti-viral and immunomodulatory activity, but there is no current clinical evidence of its effectiveness on the outcome of the disease. \u003Cem>Methods:\u003C/em> We enrolled all 18-85 years old inpatients from Central Defense Hospital, Madrid, Spain, who were hospitalised due to COVID-19 and had a definitive outcome (either dead or discharged). We used a statistical survival analysis. \u003Cem>Results:\u003C/em> We analysed 220 medical records. 166 patients met the inclusion criteria. 48,8 % of patients not treated with HCQ died, versus 22% in the group of hydroxychloroquine (p=0,002). According to clinical picture at admission, hydroxychloroquine increased the mean cumulative survival in all groups from 1,4 to 1,8 times. This difference was statistically significant in the mild group. \u003Cem>Conclusions:\u003C/em> in a cohort of 166 patients between 18 to 85 years hospitalised with COVID-19, hydroxychloroquine treatment with an initial loading dose of 800mg improved patient survival when admitted in early stages of the disease. There was a non-statistically significant trend towards survival in all groups, which will need to be clarified in subsequent studies.","COVID-19; treatment; drug; survival; antiviral; hydroxychloroquine","10.20944/preprints202005.0057.v2","2020-05-09T02:45:37Z","/frontend/manuscript/e6fa45c47a059a99319d068c163554c2/download_pub","/frontend/user/manuscript/e6fa45c47a059a99319d068c163554c2/like_toggle",[441,444,447,450,453,456,459,462,465,468,471,474,477,480,483,486,489,492,495],{"name":442,"email":443},"Francisco Javier Membrillo","javimembrillo@gmail.com",{"name":445,"email":446},"Germán Ramírez-Olivencia","germanro.76@gmail.com",{"name":448,"email":449},"Miriam Estébanez","mirestmun@gmail.com",{"name":451,"email":452},"Begoña de Dios","bdedgar@mde.es",{"name":454,"email":455},"María Dolores Herrero","mherme2@mde.es",{"name":457,"email":458},"Tatiana Mata","tmatfor@oc.mde.es",{"name":460,"email":461},"Alberto M. 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