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li{display:table-cell;float:none!important;width:10%;padding:0 15px;border-right:1px solid rgba(0,0,0,0.05)}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:last-child{border-right-width:0}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li.hide-border{border-right-width:0}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li a{border-bottom-width:0;padding:9px 15px;line-height:120%}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{font-weight:700}.rtl .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child{border-right-width:0}.rtl .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:last-child{border-right-width:1px}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu{width:980px!important;margin:0}.header-plain:not(.menuo-right) #Header .top_bar_left{width:auto!important}.header-stack.header-center #Top_bar #menu{display:inline-block!important}.header-simple #Top_bar #menu{display:none;height:auto;width:300px;bottom:auto;top:100%;right:1px;position:absolute;margin:0}.header-simple #Header a.responsive-menu-toggle{display:block;right:10px}.header-simple #Top_bar #menu > ul{width:100%;float:left}.header-simple #Top_bar #menu ul li{width:100%;padding-bottom:0;border-right:0;position:relative}.header-simple #Top_bar #menu ul li a{padding:0 20px;margin:0;display:block;height:auto;line-height:normal;border:none}.header-simple #Top_bar #menu ul li a:after{display:none}.header-simple #Top_bar #menu ul li a span{border:none;line-height:44px;display:inline;padding:0}.header-simple #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:44px;height:44px;line-height:44px;font-size:30px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33}.header-simple #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+"}.header-simple #Top_bar #menu ul li.hover > .menu-toggle:after{content:"-"}.header-simple #Top_bar #menu ul li.hover a{border-bottom:0}.header-simple #Top_bar #menu ul.mfn-megamenu li .menu-toggle{display:none}.header-simple #Top_bar #menu ul li ul{position:relative!important;left:0!important;top:0;padding:0;margin:0!important;width:auto!important;background-image:none}.header-simple #Top_bar #menu ul li ul li{width:100%!important;display:block;padding:0}.header-simple #Top_bar #menu ul li ul li a{padding:0 20px 0 30px}.header-simple #Top_bar #menu ul li ul li a .menu-arrow{display:none}.header-simple #Top_bar #menu ul li ul li a span{padding:0}.header-simple #Top_bar #menu ul li ul li a span:after{display:none!important}.header-simple #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{text-transform:uppercase;font-weight:400}.header-simple #Top_bar .menu > li > ul.mfn-megamenu > li > ul{display:block!important;position:inherit;left:auto;top:auto}.header-simple #Top_bar #menu ul li ul li ul{border-left:0!important;padding:0;top:0}.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 20px 0 40px}.rtl.header-simple #Top_bar #menu{left:1px;right:auto}.rtl.header-simple #Top_bar a.responsive-menu-toggle{left:10px;right:auto}.rtl.header-simple #Top_bar #menu ul li.submenu .menu-toggle{left:0;right:auto}.rtl.header-simple #Top_bar #menu ul li ul{left:auto!important;right:0!important}.rtl.header-simple #Top_bar #menu ul li ul li a{padding:0 30px 0 20px}.rtl.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 40px 0 20px}.menu-highlight #Top_bar .menu > li{margin:0 2px}.menu-highlight:not(.header-creative) #Top_bar .menu > li > a{margin:20px 0;padding:0;-webkit-border-radius:5px;border-radius:5px}.menu-highlight #Top_bar .menu > li > a:after{display:none}.menu-highlight #Top_bar .menu > li > a span:not(.description){line-height:50px}.menu-highlight #Top_bar .menu > li > a span.description{display:none}.menu-highlight.header-stack #Top_bar .menu > li > a{margin:10px 0!important}.menu-highlight.header-stack #Top_bar .menu > li > a span:not(.description){line-height:40px}.menu-highlight.header-transparent #Top_bar .menu > li > a{margin:5px 0}.menu-highlight.header-simple #Top_bar #menu ul li,.menu-highlight.header-creative #Top_bar #menu ul li{margin:0}.menu-highlight.header-simple #Top_bar #menu ul li > a,.menu-highlight.header-creative #Top_bar #menu ul li > a{-webkit-border-radius:0;border-radius:0}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a{margin:10px 0!important;padding:5px 0!important}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a span{line-height:30px!important}.header-modern.menu-highlight.menuo-right .menu_wrapper{margin-right:20px}.menu-line-below #Top_bar .menu > li > a:after{top:auto;bottom:-4px}.menu-line-below #Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:-4px}.menu-line-below-80 #Top_bar:not(.is-sticky) .menu > li > a:after{height:4px;left:10%;top:50%;margin-top:20px;width:80%}.menu-line-below-80-1 #Top_bar:not(.is-sticky) .menu > li > a:after{height:1px;left:10%;top:50%;margin-top:20px;width:80%}.menu-link-color #Top_bar .menu > li > a:after{display:none!important}.menu-arrow-top #Top_bar .menu > li > a:after{background:none repeat scroll 0 0 rgba(0,0,0,0)!important;border-color:#ccc transparent transparent;border-style:solid;border-width:7px 7px 0;display:block;height:0;left:50%;margin-left:-7px;top:0!important;width:0}.menu-arrow-top.header-transparent #Top_bar .menu > li > a:after,.menu-arrow-top.header-plain #Top_bar .menu > li > a:after{display:none}.menu-arrow-top #Top_bar.is-sticky .menu > li > a:after{top:0!important}.menu-arrow-bottom #Top_bar .menu > li > a:after{background:none!important;border-color:transparent transparent #ccc;border-style:solid;border-width:0 7px 7px;display:block;height:0;left:50%;margin-left:-7px;top:auto;bottom:0;width:0}.menu-arrow-bottom.header-transparent #Top_bar .menu > li > a:after,.menu-arrow-bottom.header-plain #Top_bar .menu > li > a:after{display:none}.menu-arrow-bottom #Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:0}.menuo-no-borders #Top_bar .menu > li > a span:not(.description){border-right-width:0}.menuo-no-borders #Header_creative #Top_bar .menu > li > a span{border-bottom-width:0}body.header-creative{padding-left:50px}body.header-creative.header-open{padding-left:250px}body.error404,body.under-construction,body.template-blank{padding-left:0!important}.header-creative.footer-fixed #Footer,.header-creative.footer-sliding #Footer,.header-creative.footer-stick #Footer.is-sticky{box-sizing:border-box;padding-left:50px}.header-open.footer-fixed #Footer,.header-open.footer-sliding #Footer,.header-creative.footer-stick #Footer.is-sticky{padding-left:250px}.header-rtl.header-creative.footer-fixed #Footer,.header-rtl.header-creative.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-left:0;padding-right:50px}.header-rtl.header-open.footer-fixed #Footer,.header-rtl.header-open.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-right:250px}#Header_creative{background:#fff;position:fixed;width:250px;height:100%;left:-200px;top:0;z-index:9002;-webkit-box-shadow:2px 0 4px 2px rgba(0,0,0,.15);box-shadow:2px 0 4px 2px rgba(0,0,0,.15)}#Header_creative .container{width:100%}#Header_creative .creative-wrapper{opacity:0;margin-right:50px}#Header_creative a.creative-menu-toggle{display:block;width:34px;height:34px;line-height:34px;font-size:22px;text-align:center;position:absolute;top:10px;right:8px;border-radius:3px}.admin-bar #Header_creative a.creative-menu-toggle{top:42px}#Header_creative #Top_bar{position:static;width:100%}#Header_creative #Top_bar .top_bar_left{width:100%!important;float:none}#Header_creative #Top_bar .top_bar_right{width:100%!important;float:none;height:auto;margin-bottom:35px;text-align:center;padding:0 20px;top:0;-webkit-box-sizing:border-box;-moz-box-sizing:border-box;box-sizing:border-box}#Header_creative #Top_bar .top_bar_right:before{display:none}#Header_creative #Top_bar .top_bar_right_wrapper{top:0}#Header_creative #Top_bar .logo{float:none;text-align:center;margin:15px 0}#Header_creative #Top_bar .menu_wrapper{float:none;margin:0 0 30px}#Header_creative #Top_bar .menu > li{width:100%;float:none;position:relative}#Header_creative #Top_bar .menu > li > a{padding:0;text-align:center}#Header_creative #Top_bar .menu > li > a:after{display:none}#Header_creative #Top_bar .menu > li > a span{border-right:0;border-bottom-width:1px;line-height:38px}#Header_creative #Top_bar .menu li ul{left:100%;right:auto;top:0;box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03);-webkit-box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03)}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu{width:700px!important}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu > li > ul{left:0}#Header_creative #Top_bar .menu li ul li a{padding-top:9px;padding-bottom:8px}#Header_creative #Top_bar .menu li ul li ul{top:0!important}#Header_creative #Top_bar .menu > li > a span.description{display:block;font-size:13px;line-height:28px!important;clear:both}#Header_creative #Top_bar .search_wrapper{left:100%;top:auto;bottom:0}#Header_creative #Top_bar a#header_cart{display:inline-block;float:none;top:3px}#Header_creative #Top_bar a#search_button{display:inline-block;float:none;top:3px}#Header_creative #Top_bar .wpml-languages{display:inline-block;float:none;top:0}#Header_creative #Top_bar .wpml-languages.enabled:hover a.active{padding-bottom:9px}#Header_creative #Top_bar a.button.action_button{display:inline-block;float:none;top:16px;margin:0}#Header_creative #Top_bar .banner_wrapper{display:block;text-align:center}#Header_creative #Top_bar .banner_wrapper img{max-width:100%;height:auto;display:inline-block}#Header_creative #Action_bar{display:none;position:absolute;bottom:0;top:auto;clear:both;padding:0 20px;box-sizing:border-box}#Header_creative #Action_bar .social{float:none;text-align:center;padding:5px 0 15px}#Header_creative #Action_bar .social li{margin-bottom:2px}#Header_creative .social li a{color:rgba(0,0,0,.5)}#Header_creative .social li a:hover{color:#000}#Header_creative .creative-social{position:absolute;bottom:10px;right:0;width:50px}#Header_creative .creative-social li{display:block;float:none;width:100%;text-align:center;margin-bottom:5px}.header-creative .fixed-nav.fixed-nav-prev{margin-left:50px}.header-creative.header-open .fixed-nav.fixed-nav-prev{margin-left:250px}.menuo-last #Header_creative #Top_bar .menu li.last ul{top:auto;bottom:0}.header-open #Header_creative{left:0}.header-open #Header_creative .creative-wrapper{opacity:1;margin:0!important}.header-open #Header_creative .creative-menu-toggle,.header-open #Header_creative .creative-social{display:none}body.header-rtl.header-creative{padding-left:0;padding-right:50px}.header-rtl #Header_creative{left:auto;right:-200px}.header-rtl.nice-scroll #Header_creative{margin-right:10px}.header-rtl #Header_creative .creative-wrapper{margin-left:50px;margin-right:0}.header-rtl #Header_creative a.creative-menu-toggle{left:8px;right:auto}.header-rtl #Header_creative .creative-social{left:0;right:auto}.header-rtl #Footer #back_to_top.sticky{right:125px}.header-rtl #popup_contact{right:70px}.header-rtl #Header_creative #Top_bar .menu li ul{left:auto;right:100%}.header-rtl #Header_creative #Top_bar .search_wrapper{left:auto;right:100%}.header-rtl .fixed-nav.fixed-nav-prev{margin-left:0!important}.header-rtl .fixed-nav.fixed-nav-next{margin-right:50px}body.header-rtl.header-creative.header-open{padding-left:0;padding-right:250px!important}.header-rtl.header-open #Header_creative{left:auto;right:0}.header-rtl.header-open #Footer #back_to_top.sticky{right:325px}.header-rtl.header-open #popup_contact{right:270px}.header-rtl.header-open .fixed-nav.fixed-nav-next{margin-right:250px}#Header_creative.active{left:-1px}.header-rtl #Header_creative.active{left:auto;right:-1px}#Header_creative.active .creative-wrapper{opacity:1;margin:0}.header-creative .vc_row[data-vc-full-width]{padding-left:50px}.header-creative.header-open .vc_row[data-vc-full-width]{padding-left:250px}.header-open .vc_parallax .vc_parallax-inner{left:auto;width:calc(100% - 250px)}.header-open.header-rtl .vc_parallax .vc_parallax-inner{left:0;right:auto}#Header_creative.scroll{height:100%;overflow-y:auto}#Header_creative.scroll:not(.dropdown) .menu li ul{display:none!important}#Header_creative.scroll #Action_bar{position:static}#Header_creative.dropdown{outline:none}#Header_creative.dropdown #Top_bar .menu_wrapper{float:left}#Header_creative.dropdown #Top_bar #menu ul li{position:relative;float:left}#Header_creative.dropdown #Top_bar #menu ul li a:after{display:none}#Header_creative.dropdown #Top_bar #menu ul li a span{line-height:38px;padding:0}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:38px;height:38px;line-height:38px;font-size:26px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+"}#Header_creative.dropdown #Top_bar #menu ul li.hover > .menu-toggle:after{content:"-"}#Header_creative.dropdown #Top_bar #menu ul li.hover a{border-bottom:0}#Header_creative.dropdown #Top_bar #menu ul.mfn-megamenu li .menu-toggle{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul{position:relative!important;left:0!important;top:0;padding:0;margin-left:0!important;width:auto!important;background-image:none}#Header_creative.dropdown #Top_bar #menu ul li ul li{width:100%!important}#Header_creative.dropdown #Top_bar #menu ul li ul li a{padding:0 10px;text-align:center}#Header_creative.dropdown #Top_bar #menu ul li ul li a .menu-arrow{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul li a span{padding:0}#Header_creative.dropdown #Top_bar #menu ul li ul li a span:after{display:none!important}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{text-transform:uppercase;font-weight:400}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu > li > ul{display:block!important;position:inherit;left:auto;top:auto}#Header_creative.dropdown #Top_bar #menu ul li ul li ul{border-left:0!important;padding:0;top:0}#Header_creative{transition:left .5s ease-in-out,right .5s ease-in-out}#Header_creative .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out .5s}#Header_creative.active .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out}}@media only screen and (min-width:1130px){#Top_bar.is-sticky{position:fixed!important;width:100%;left:0;top:-60px;height:60px;z-index:701;background:#fff;opacity:.97;filter:alpha(opacity = 97);-webkit-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);-moz-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);box-shadow:0 2px 5px 0 rgba(0,0,0,0.1)}.layout-boxed.header-boxed #Top_bar.is-sticky{max-width:1130px;left:50%;-webkit-transform:translateX(-50%);transform:translateX(-50%)}.layout-boxed.header-boxed.nice-scroll #Top_bar.is-sticky{margin-left:-5px}#Top_bar.is-sticky .top_bar_left,#Top_bar.is-sticky .top_bar_right,#Top_bar.is-sticky .top_bar_right:before{background:none}#Top_bar.is-sticky .top_bar_right{top:-4px;height:auto}#Top_bar.is-sticky .top_bar_right_wrapper{top:15px}.header-plain #Top_bar.is-sticky .top_bar_right_wrapper{top:0}#Top_bar.is-sticky .logo{width:auto;margin:0 30px 0 20px;padding:0}#Top_bar.is-sticky #logo{padding:5px 0!important;height:50px!important;line-height:50px!important}.logo-no-sticky-padding #Top_bar.is-sticky #logo{height:60px!important;line-height:60px!important}#Top_bar.is-sticky #logo img.logo-main{display:none}#Top_bar.is-sticky #logo img.logo-sticky{display:inline;max-height:35px}#Top_bar.is-sticky .menu_wrapper{clear:none}#Top_bar.is-sticky .menu_wrapper .menu > li > a{padding:15px 0}#Top_bar.is-sticky .menu > li > a,#Top_bar.is-sticky .menu > li > a span{line-height:30px}#Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:-4px}#Top_bar.is-sticky .menu > li > a span.description{display:none}#Top_bar.is-sticky .secondary_menu_wrapper,#Top_bar.is-sticky .banner_wrapper{display:none}.header-overlay #Top_bar.is-sticky{display:none}.sticky-dark #Top_bar.is-sticky{background:rgba(0,0,0,.8)}.sticky-dark #Top_bar.is-sticky #menu{background:rgba(0,0,0,.8)}.sticky-dark #Top_bar.is-sticky .menu > li > a{color:#fff}.sticky-dark #Top_bar.is-sticky .top_bar_right a{color:rgba(255,255,255,.5)}.sticky-dark #Top_bar.is-sticky .wpml-languages a.active,.sticky-dark #Top_bar.is-sticky .wpml-languages ul.wpml-lang-dropdown{background:rgba(0,0,0,0.3);border-color:rgba(0,0,0,0.1)}}@media only screen and (min-width:768px) and (max-width:1130px){.header_placeholder{height:0!important}}@media only screen and (max-width:1129px){#Top_bar #menu{display:none;height:auto;width:100%;bottom:auto;top:100%;right:1px;position:absolute;margin:0}#Top_bar a.responsive-menu-toggle{display:block}#Top_bar #menu > ul{width:100%;float:left}#Top_bar #menu ul li{width:100%;padding-bottom:0;border-right:0;position:relative}#Top_bar #menu ul li a{padding:0 25px;margin:0;display:block;height:auto;line-height:normal;border:none}#Top_bar #menu ul li a:after{display:none}#Top_bar #menu ul li a span{border:none;line-height:44px;display:inline;padding:0}#Top_bar #menu ul li a span.description{margin:0 0 0 5px}#Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:15px;top:0;width:44px;height:44px;line-height:44px;font-size:30px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33}#Top_bar #menu ul li.submenu .menu-toggle:after{content:"+"}#Top_bar #menu ul li.hover > 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href="/le220-plus-focused-ultrasonicator-500569"><span class="ubermenu-target-title ubermenu-target-text">LE220-plus</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">High-throughput, 1 to 96 tube capacity</span></a></li><li id="menu-item-26916" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26916 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/le220r-plus-focused-ultrasonicator-500578"><span class="ubermenu-target-title ubermenu-target-text">LE220R-plus</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Robotic version of the LE220-plus</span></a></li><li id="menu-item-30065" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-30065 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/le220rsc-focused-ultrasonicator"><span class="ubermenu-target-title ubermenu-target-text">LE220Rsc</span></a></li></ul></li><li id="menu-item-26917" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26917 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/on-deck-automation-focused-ultrasonicators"><span class="ubermenu-target-title ubermenu-target-text">On-Deck Automation Focused-ultrasonicators</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26917 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26918" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26918 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/r230-focused-ultrasonicator"><span class="ubermenu-target-title ubermenu-target-text">R230</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">On-deck, next generation Focused-ultrasonicator</span></a></li></ul></li><li id="menu-item-26919" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26919 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/cryoprep"><span class="ubermenu-target-title ubermenu-target-text">cryoPREP<sup>®</sup> Extraction Systems</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26919 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26920" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26920 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/cp01-cryoprep-manual-dry-pulverizer-500230"><span class="ubermenu-target-title ubermenu-target-text">CP01 Manual Pulverizer</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">For Tissues 15 mg to 2 g</span></a></li><li id="menu-item-26921" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26921 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/cp02-cryoprep-automated-dry-pulverizer-110v-500001"><span class="ubermenu-target-title ubermenu-target-text">CP02 Automated Dry Pulverizer</span></a></li><li id="menu-item-26922" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26922 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/cp03-cryoprep-manual-dry-pulverizer-500049"><span class="ubermenu-target-title ubermenu-target-text">CP03 Manual Pulverizer</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">For Tissues 2 to 5 g</span></a></li></ul></li><li id="menu-item-26923" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26923 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/sonolab-software"><span class="ubermenu-target-title ubermenu-target-text">SonoLab Software</span></a></li></ul></li><li id="menu-item-26927" class="scroll-menu-top ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26927 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents"><span class="ubermenu-target-title ubermenu-target-text">Kits & Reagents</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26927 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-41083" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-41083 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/truprep-protein/"><span class="ubermenu-target-title ubermenu-target-text">truPREP® Protein Kits</span></a></li><li id="menu-item-35108" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-35108 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/truxtrac-ffpe-na-auto-96-kits"><span class="ubermenu-target-title ubermenu-target-text">truXTRAC® FFPE SMART Solutions</span></a></li><li id="menu-item-26931" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26931 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/cryoprep-0"><span class="ubermenu-target-title ubermenu-target-text">cryoPREP Starter Kits</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">cryoPREP Sample Tubes & Holders</span></a></li><li id="menu-item-26933" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26933 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/nucleic-acids-shearing-kits-and-buffers"><span class="ubermenu-target-title ubermenu-target-text">Nucleic Acids Shearing Kits and Buffers</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">truSHEAR, truPOP, DNA Verification Kits</span></a></li><li id="menu-item-26929" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26929 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/truxtrac-ffpe-kits"><span class="ubermenu-target-title ubermenu-target-text">Other truXTRAC<sup>®</sup> FFPE Kits</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">FFPE Biomolecule Extraction Kits</span></a></li><li id="menu-item-26934" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26934 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/truxtrac-other"><span class="ubermenu-target-title ubermenu-target-text">Other truXTRAC<sup>®</sup> Kits</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">cfDNA, DBS, MALDI-TOF</span></a></li><li id="menu-item-26935" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26935 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/protein-extraction-buffers"><span class="ubermenu-target-title ubermenu-target-text">Protein Extraction Buffers</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Native, Total Protein, & Detergent-free Buffers</span></a></li><li id="menu-item-26930" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26930 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/truchip"><span class="ubermenu-target-title ubermenu-target-text">truChIP<sup>®</sup></span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Chromatin Shearing Kits</span></a></li></ul></li><li id="menu-item-26936" class="scroll-menu-top ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26936 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables"><span class="ubermenu-target-title ubermenu-target-text">Consumables – Tubes and Vials</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26936 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26938" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26938 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/afa-tube"><span class="ubermenu-target-title ubermenu-target-text">AFA-TUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Laser etched polymer vessel for high-throughput workflows</span></a></li><li id="menu-item-26939" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26939 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/microtube"><span class="ubermenu-target-title ubermenu-target-text">microTUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Small sample volumes, 130 µl or less</span></a></li><li id="menu-item-26940" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26940 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/millitube"><span class="ubermenu-target-title ubermenu-target-text">milliTUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">1 ml or 2 ml sample volume. Good for chromatin shearing</span></a></li><li id="menu-item-26942" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26942 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/other-afa-tubes"><span class="ubermenu-target-title ubermenu-target-text">Other AFA-TUBEs</span></a></li><li id="menu-item-26943" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26943 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/afa-process-vessels/"><span class="ubermenu-target-title ubermenu-target-text">AFA Process Vessels</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">High power, low temperature processing</span></a></li><li id="menu-item-26944" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26944 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/g-tube"><span class="ubermenu-target-title ubermenu-target-text">g-TUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">DNA shearing fragments 6 to 20 kb</span></a></li></ul></li><li id="menu-item-26945" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26945 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/cryoprep-consumables"><span class="ubermenu-target-title ubermenu-target-text">cryoPREP Consumables</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26945 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26947" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26947 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/cryoprep-consumables/tissuetube"><span class="ubermenu-target-title ubermenu-target-text">tissueTUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Tissue processing devices</span></a></li></ul></li><li id="menu-item-26949" class="scroll-menu-top ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26949 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories"><span class="ubermenu-target-title ubermenu-target-text">Accessories</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26949 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26952" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26952 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/automation-accessories"><span class="ubermenu-target-title ubermenu-target-text">Automation Accessories</span></a></li><li id="menu-item-41270" class="ubermenu-item ubermenu-item-type-post_type ubermenu-item-object-page ubermenu-item-41270 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/r230-accessories"><span class="ubermenu-target-title ubermenu-target-text">R230 Accessories</span></a></li><li id="menu-item-26953" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26953 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/m220-accessories"><span class="ubermenu-target-title ubermenu-target-text">M220 Accessories</span></a></li><li id="menu-item-26954" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26954 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/me220-accessories"><span class="ubermenu-target-title ubermenu-target-text">ME220 Accessories</span></a></li><li id="menu-item-30718" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-30718 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/ml230-accessories"><span class="ubermenu-target-title ubermenu-target-text">ML230 Accessories</span></a></li><li id="menu-item-26955" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26955 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/s220-accessories"><span class="ubermenu-target-title ubermenu-target-text">S220 Accessories</span></a></li><li id="menu-item-26956" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26956 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/e220-evolution-accessories"><span class="ubermenu-target-title ubermenu-target-text">E220<i>evolution</i> Accessories</span></a></li><li id="menu-item-26957" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26957 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/e220-accessories"><span class="ubermenu-target-title ubermenu-target-text">E220 Accessories</span></a></li><li id="menu-item-26958" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26958 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/l-series-accessories"><span class="ubermenu-target-title ubermenu-target-text">L-Series Accessories</span></a></li><li id="menu-item-26959" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26959 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/cp01-accessories"><span class="ubermenu-target-title ubermenu-target-text">CP01 Accessories</span></a></li><li id="menu-item-26960" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26960 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/cp02-accessories"><span class="ubermenu-target-title ubermenu-target-text">CP02 Accessories</span></a></li><li id="menu-item-26961" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26961 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/cp03-accessories"><span class="ubermenu-target-title ubermenu-target-text">CP03 Accessories</span></a></li><li id="menu-item-26963" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26963 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/water-conditioning-system-accessories"><span class="ubermenu-target-title ubermenu-target-text">Water Conditioning System Accessories</span></a></li><li id="menu-item-26964" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26964 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/other-accessories"><span class="ubermenu-target-title ubermenu-target-text">Other Accessories</span></a></li></ul></li><li id="menu-item-26965" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26965 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop 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!important;}.vc_custom_1587936235461{margin-top: 13px !important;margin-bottom: 0px !important;}.vc_custom_1583492989135{padding-right: 20px !important;padding-left: 20px !important;}.vc_custom_1587132937230{margin-bottom: 8px !important;padding-bottom: 8px !important;}</style> <div id="Content" class="cms_mage_page" style="padding: 0px;"> <div class="content_wrapper clearfix"> <div class="sections_group"> <div class="entry-content" itemprop="mainContentOfPage"> <div data-vc-full-width="true" data-vc-full-width-init="false" class="vc_row wpb_row vc_row-fluid shearing_banner vc_custom_1583475962885 vc_row-has-fill"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner vc_custom_1583475591897"><div class="wpb_wrapper"><h1 style="color: #ffffff;text-align: center" class="vc_custom_heading vc_do_custom_heading vc_custom_1646378926666" >Adaptive Focused Acoustics<sup>®</sup>(AFA<sup>®</sup>) for Cell Lysis</h1> <div class="wpb_text_column wpb_content_element" > <div class="wpb_wrapper"> <h3 class="vc_custom_heading" style="color: #ffffff; text-align: center;">Cell lysis methods differ greatly depending on the species the cells are derived from. Covaris <a href="/afa-technology/">Adaptive Focused Acoustics<sup class="reg">®</sup>(AFA<sup class="reg">®</sup>)</a> is unique in its ability to control the amount of energy delivered to samples making it ideal for sonication cell lysis workflows.</h3> </div> </div> </div></div></div></div><div class="vc_row-full-width vc_clearfix"></div><div class="vc_row wpb_row vc_row-fluid cmspage_menu vc_custom_1583489874470 vc_column-gap-20"><div class="common-pages-left-container PrdMenu clearfix wpb_column vc_column_container vc_col-sm-3 vc_col-has-fill"><div class="vc_column-inner vc_custom_1583479740629"><div class="wpb_wrapper"><h4 style="color: #ffffff;text-align: left" class="vc_custom_heading vc_do_custom_heading custom_h4 vc_custom_1583481599852" >Contents</h4> <div class="wpb_text_column wpb_content_element" > <div class="wpb_wrapper"> <ul id="shearing-sidebar"> <li class="active"><a style="cursor: pointer; display: block; text-decoration: none;" href="#overview">Overview</a></li> <li><a style="cursor: pointer; display: block; text-decoration: none;" href="#downstream_workflow">Downstream Multiomics Workflows:<br /> </a></li> <li class=""><a style="cursor: pointer; display: block; text-decoration: none;" href="#cell_lysis_workflow">Covaris Cell Lysis Workflows<br /> </a></li> <li class=""><a style="cursor: pointer; display: block; text-decoration: none;" href="#featured_product">Featured Products</a></li> <li class=""><a style="cursor: pointer; display: grid; text-decoration: none;" href="#resources">Resources</a></li> <li class=""><a style="cursor: pointer; display: grid; text-decoration: none;" href="#references">References</a></li> </ul> </div> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-9"><div class="vc_column-inner vc_custom_1580898106712"><div class="wpb_wrapper"><div id="overview" class="vc_row wpb_row vc_inner vc_row-fluid vc_custom_1583494105376"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"><h2 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading" >Robust extraction workflow solutions for cells lysis Powered by AFA®</h2> <div class="wpb_text_column wpb_content_element vc_custom_1583740338895 custom_h5" > <div class="wpb_wrapper"> <h5>Covaris AFA technology delivers highly tunable energy through Focused-ultrasonicator instruments. By controlling the dosage of acoustic energy delivered, it is possible to gently disrupt the cell membranes of mammalian cells (e.g., GPCR assays), or abruptly disrupt the cell walls of bacteria (e.g., total protein extraction). AFA provides the flexibility to homogenize cells and efficiently extract proteins, RNA, DNA, Metabolites, or other targets in buffers optimized for downstream analytical methods.</h5> </div> </div> </div></div></div></div><div id="downstream_workflow" class="vc_row wpb_row vc_inner vc_row-fluid vc_custom_1583741987617"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"><h2 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading" >Downstream Multiomics Workflows:</h2> <div class="wpb_text_column wpb_content_element vc_custom_1729771333376 custom_h5 mobile_padding" > <div class="wpb_wrapper"> <p><a class="image-popup-no-margins" href="https://www.covaris.com/wp/wp-content/uploads/2020/03/IntegratedMultiomics.jpg"><br /> <img class="alignnone" src="https://www.covaris.com/wp/wp-content/uploads/2020/03/IntegratedMultiomics.jpg" alt="Cell Lysis: Downstream Multiomics Workflows" width="700" height="573" /><br /> </a></p> </div> </div> </div></div></div></div><div id="cell_lysis_workflow" class="vc_row wpb_row vc_inner vc_row-fluid"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"><h2 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading" >Covaris Cell Lysis Workflows</h2> <div class="wpb_text_column wpb_content_element vc_custom_1730978555677 custom_h5 mobile_padding" > <div class="wpb_wrapper"> <h5>A. Direct cell lysis</h5> <p> </p> <p><a class="custom_h5 mobile_padding image-popup-no-margins" href="https://www.covaris.com/wp/wp-content/uploads/2020/03/Proteomics_v4.jpg"><img class="alignnone" src="https://www.covaris.com/wp/wp-content/uploads/2020/03/Proteomics_v4.jpg" alt="Flow chart showing Cell Lysis Workflows - Direct cell lysis | high pressure homogenizer cell lysis" width="700" height="77" /></a></p> <h5>B. Dry tissue pulverization extraction workflow using cryoPREP and AFA</h5> <p> </p> <p><a class="custom_h5 mobile_padding image-popup-no-margins" href="https://www.covaris.com/wp/wp-content/uploads/2020/03/Proteomics_v5.jpg"><img class="alignnone" src="https://www.covaris.com/wp/wp-content/uploads/2020/03/Proteomics_v5.jpg" alt="Flow chart showing Dry tissue pulverization extraction workflow using cryoPREP and AFA | Covaris Cell Lysis Workflows" width="700" height="77" /></a></p> </div> </div> </div></div></div></div></div></div></div></div><div id="featured_product" class="vc_row wpb_row vc_row-fluid cmspage_menu vc_custom_1583494558716"><div class="wpb_column vc_column_container vc_col-sm-3"><div class="vc_column-inner"><div class="wpb_wrapper"></div></div></div><div class="wpb_column vc_column_container vc_col-sm-9"><div class="vc_column-inner"><div class="wpb_wrapper"><h2 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading vc_custom_1583493877217" >Featured Products</h2><div class="vc_row wpb_row vc_inner vc_row-fluid instrument_lev_1 vc_custom_1583494563486 vc_row-o-equal-height vc_row-flex"><div class="wpb_column vc_column_container vc_col-sm-4"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element vc_custom_1594993929537" > <div class="wpb_wrapper"> <p><img class="alignnone size-full wp-image-15259" src="https://www.covaris.com/wp/wp-content/uploads/2020/07/CP02.jpg" alt="" width="200" height="168" /></p> </div> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-8"><div class="vc_column-inner"><div class="wpb_wrapper"><h4 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading vc_custom_1591193097409" >cryoPREP® Dry Pulverizers</h4><h6 style="color: #020202;text-align: left" class="vc_custom_heading vc_do_custom_heading" >cryoPREP® Dry Pulverizers deliver a calibrated and repeatable mechanical impact to cryofracture flash-frozen samples without direct sample contact.</h6><div class="vc_btn3-container vc_btn3-inline vc_custom_1591267868809 vc_do_btn" ><a style="background-color:#ededed; color:#015cb8;" class="vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-rounded vc_btn3-style-custom vc_btn3-icon-right" href="/cp02-cryoprep-automated-dry-pulverizer-110v-500001" title="">READ MORE <i class="vc_btn3-icon fas fa-arrow-left"></i></a></div></div></div></div></div><div class="vc_row wpb_row vc_inner vc_row-fluid instrument_lev_1 vc_custom_1583494563486 vc_row-o-equal-height vc_row-flex"><div class="wpb_column vc_column_container vc_col-sm-4"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element vc_custom_1583741294581" > <div class="wpb_wrapper"> <p><img class="alignnone size-full wp-image-15468" src="https://www.covaris.com/wp/wp-content/uploads/2020/02/Buffer_Bottle.jpg" alt="" width="136" height="200" /></p> </div> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-8"><div class="vc_column-inner"><div class="wpb_wrapper"><h4 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading vc_custom_1583741208445" >Protein Extraction Buffers</h4><h6 style="color: #020202;text-align: left" class="vc_custom_heading vc_do_custom_heading" >A range of extraction buffers for the efficient protein extraction from cells and tissues optimized for your downstream application.</h6><div class="vc_btn3-container vc_btn3-inline vc_custom_1591358649994 vc_do_btn" ><a style="background-color:#ededed; color:#015cb8;" class="vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-rounded vc_btn3-style-custom vc_btn3-icon-right" href="/products-services/kits-reagents/protein-extraction-buffers" title="">READ MORE <i class="vc_btn3-icon fas fa-arrow-left"></i></a></div></div></div></div></div><div class="vc_row wpb_row vc_inner vc_row-fluid instrument_lev_1 vc_custom_1583494563486 vc_row-o-equal-height vc_row-flex"><div class="wpb_column vc_column_container vc_col-sm-4"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element vc_custom_1583741487897" > <div class="wpb_wrapper"> <p><img class="size-full wp-image-15527" src="https://www.covaris.com/wp/wp-content/uploads/2020/02/ChIPKit_blurred-1.jpg" alt="" width="200" height="134" /></p> </div> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-8"><div class="vc_column-inner"><div class="wpb_wrapper"><h4 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading vc_custom_1591127976194" >truPOP® Cell Lysis Buffer</h4><h6 style="color: #020202;text-align: left" class="vc_custom_heading vc_do_custom_heading" >Buffers for extracting high molecular weight DNA and RNA from a variety of sample types.</h6><div class="vc_btn3-container vc_btn3-inline vc_custom_1591269686877 vc_do_btn" ><a style="background-color:#ededed; color:#015cb8;" class="vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-rounded vc_btn3-style-custom vc_btn3-icon-right" href="/trupop-cell-lysis-buffer-520268" title="">READ MORE <i class="vc_btn3-icon fas fa-arrow-left"></i></a></div></div></div></div></div></div></div></div></div><div id="resources" class="vc_row wpb_row vc_row-fluid cmspage_menu category_resources vc_custom_1587134484406"><div class="wpb_column vc_column_container vc_col-sm-3"><div class="vc_column-inner"><div class="wpb_wrapper"></div></div></div><div class="wpb_column vc_column_container vc_col-sm-9"><div class="vc_column-inner vc_custom_1583492989135"><div class="wpb_wrapper"><h2 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading vc_custom_1579697651435" >Resources</h2><div class="vc_row wpb_row vc_inner vc_row-fluid vc_custom_1583492996693 vc_row-o-equal-height vc_row-flex"><div class="sidebar_resources wpb_column vc_column_container vc_col-sm-6"><div class="vc_column-inner"><div class="wpb_wrapper"><h5 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading custom_h5 vc_custom_1589892628087" >Cell Lysis Application Notes</h5><h6 style="color: #020202;text-align: left" class="vc_custom_heading vc_do_custom_heading custom_h5 vc_custom_1589892650542" >Application notes to support your cell lysis workflows including mammalian cell lysis, bacterial cell lysis, HMW extraction and more.</h6><div class="vc_btn3-container vc_btn3-inline vc_custom_1587936235461 vc_do_btn" ><a style="background-color:#ffffff; color:#015cb8;" class="vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-rounded vc_btn3-style-custom" href="/application-technical-notes?filter_tag=Cell%20Lysis" title="">LEARN MORE</a></div></div></div></div></div></div></div></div></div><div id="references" class="vc_row wpb_row vc_row-fluid cmspage_menu category_resources vc_custom_1587132924598"><div class="wpb_column vc_column_container vc_col-sm-3"><div class="vc_column-inner"><div class="wpb_wrapper"></div></div></div><div class="wpb_column vc_column_container vc_col-sm-9"><div class="vc_column-inner vc_custom_1583492989135"><div class="wpb_wrapper"><h2 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading vc_custom_1587132937230" >References</h2> <div class="wpb_text_column wpb_content_element" > <div class="wpb_wrapper"> <h4><strong>E.coli</strong></h4> <ol> <li><a href="https://www.ncbi.nlm.nih.gov/pubmed/23475508" target="_blank" rel="noopener noreferrer">Li, Q., Mannall, G. J., Ali, S., & Hoare, M. (2013). An ultra scale-down approach to study the interaction of fermentation, homogenization, and centrifugation for antibody fragment recovery from recE. coli. Biotechnology and Bioengineering, 110(8), 2150-2160. doi:10.1002/bit.24891</a></li> <li><a href="https://www.ncbi.nlm.nih.gov/pubmed/22383367" target="_blank" rel="noopener noreferrer">Li, Q., Aucamp, J. P., Tang, A., Chatel, A., & Hoare, M. (2012). Use of focused acoustics for cell disruption to provide ultra scale-down insights of microbial homogenization and its bioprocess impact-recovery of antibody fragments from rec E. coli. Biotechnology and Bioengineering, 109(8), 2059-2069. doi:10.1002/bit.24484</a></li> <li><a href="https://www.covaris.com/blogs/distributors/finland/" target="_blank" rel="noopener noreferrer">Perez-Pardo, M. A., Ali, S., Balasundaram, B., Mannall, G. J., Baganz, F., & Bracewell, D. G. (2011). Assessment of the manufacturability of Escherichia coli high cell density fermentations. Biotechnology Progress, 27(5), 1488-1496. doi:10.1002/btpr.644</a></li> <li><a href="http://discovery.ucl.ac.uk/1395998/" target="_blank" rel="noopener noreferrer">Ordidge, G. C. Rapid microscale evaluation of the impact of fermentation conditions on inclusion body formation, solubilization and protein refolding yields (Unpublished doctoral dissertation). University College London (University of London).</a></li> <li><a href="https://www.covaris.com/wp-content/uploads/AFA-Release-of-Microbial-Cell-AppNote.pdf" target="_blank" rel="noopener noreferrer">Release of Microbial Cells from a Solid Matrix, Cell Lysis, and Shearing of Nucleic Acids Performed in a One-Step Procedure</a></li> </ol> </div> </div> <div class="wpb_text_column wpb_content_element" > <div class="wpb_wrapper"> <h4><strong>Mammalian Cell lysis</strong></h4> <ol> <li><a href="https://www.biorxiv.org/content/early/2017/01/23/102681" target="_blank" rel="noopener noreferrer">Budnik, B., Levy, E., & Slavov, N. (2017). Mass-spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation. doi:10.7287/peerj.preprints.2767</a></li> <li><a href="https://pubs.acs.org/doi/abs/10.1021/acs.jproteome.5b00547" target="_blank" rel="noopener noreferrer">Dhabaria, A., Cifani, P., Reed, C., Steen, H., & Kentsis, A. (2015). A High-Efficiency Cellular Extraction System for Biological Proteomics. Journal of Proteome Research, 14(8), 3403-3408. doi:10.1021/acs.jproteome.5b00547</a></li> <li><a href="https://www.ncbi.nlm.nih.gov/pubmed/25755294" target="_blank" rel="noopener noreferrer">Li, S., Plouffe, B. D., Belov, A. M., Ray, S., Wang, X., Murthy, S. K., . . . Ivanov, A. R. (2015). An Integrated Platform for Isolation, Processing, and Mass Spectrometry-based Proteomic Profiling of Rare Cells in Whole Blood. Molecular & Cellular Proteomics, 14(6), 1672-1683. doi:10.1074/mcp.m114.045724</a></li> <li><a href="https://www.ncbi.nlm.nih.gov/pubmed/24862191" target="_blank" rel="noopener noreferrer">Ly, M., Lu, F., Maheshwari, G., & Subramanian, S. (2014). Microscale acoustic disruption of mammalian cells for intracellular product release. Journal of Biotechnology, 184, 146-153. doi:10.1016/j.jbiotec.2014.04.030</a></li> </ol> </div> </div> <div class="wpb_text_column wpb_content_element" > <div class="wpb_wrapper"> <h4><strong>Yeast Cell Lysis</strong></h4> <ol> <li><a href="https://www.ncbi.nlm.nih.gov/pubmed/28884522" target="_blank" rel="noopener noreferrer">Bláha BAF, Morris SA, Ogonah OW, et al. Development of a high-throughput microscale cell disruption platform for Pichia pastoris in rapid bioprocess design. Biotechnology Progress. 2017;34(1):130-140. doi:10.1002/btpr.2555.</a></li> <li><a href="https://www.ncbi.nlm.nih.gov/pubmed/18410155" target="_blank" rel="noopener noreferrer">Wenger M, Dephillips P, Bracewell D. A Microscale Yeast Cell Disruption Technique for Integrated Process Development Strategies. Biotechnology Progress. 2008;24(3):606-614. doi:10.1021/bp070359s.</a></li> <li><a href="https://www.covaris.com/wp-content/uploads/cvAppNoteYAN-1-Rev-050814-4.pdf" target="_blank" rel="noopener noreferrer">Application Note: Active Extraction of Native Proteins from Yeast using Covaris Adaptive Focused Acoustics™ (AFA)</a></li> <li>Application Note: <a href="https://www.covaris.com/wp/wp-content/uploads/2020/06/AppNote_YAN-2_M020003RevA_Final-1.pdf" target="_blank" rel="noopener noreferrer">Protein Extraction from Yeast: Comparison of the Covaris Adaptive Focused Acoustics™ (AFA) Process to Conventional Bead Beating and Probe Sonication</a></li> </ol> </div> </div> <div class="wpb_text_column wpb_content_element" > <div class="wpb_wrapper"> <h4><strong>Pseudomonas disruption</strong></h4> <h5 class="custom_h5">Application Note:<a href="https://www.covaris.com/appnote_pseudomona_m020002reva_final/" target="_blank" rel="noopener noreferrer"> Effective disruption of Pseudomonas aeruginosa biofilms using Adaptive Focused Acoustics™ (AFA)</a></h5> <h5><strong>Plant Cells</strong></h5> <ol> <li><a href="https://www.ncbi.nlm.nih.gov/pubmed/18543963" target="_blank" rel="noopener noreferrer">Toorchi, M., Nouri, M., Tsumura, M., & Komatsu, S. (2008). Acoustic Technology for High-Performance Disruption and Extraction of Plant Proteins. Journal of Proteome Research, 7(7), 3035-3041. doi:10.1021/pr800012c</a></li> <li><a href="https://www.ncbi.nlm.nih.gov/pubmed/21367437" target="_blank" rel="noopener noreferrer">Kota, U., & Goshe, M. B. (2011). Advances in qualitative and quantitative plant membrane proteomics. Phytochemistry, 72(10), 1040-1060. doi:10.1016/j.phytochem.2011.01.027</a></li> </ol> <h5><strong>Hydrogels</strong></h5> <ol> <li><a href="https://www.ncbi.nlm.nih.gov/pubmed/29235971" target="_blank" rel="noopener noreferrer">Burgess, K. A., Miller, A. F., Oceandy, D., & Saiani, A. (2017). Western blot analysis of cells encapsulated in self-assembling peptide hydrogels. BioTechniques, 63(6). doi:10.2144/000114617</a></li> </ol> <h5><strong>CryoPREP</strong></h5> <ol> <li><a href="https://www.ncbi.nlm.nih.gov/pubmed/29472518" target="_blank" rel="noopener noreferrer">Mundt, F., Rajput, S., Li, S., Ruggles, K. V., Mooradian, A. D., Mertins, P., . . . Ma, C. X. (2018). Mass spectrometry-based proteomics reveals potential roles of NEK9 and MAP2K4 in resistance to PI3K inhibitors in triple negative breast cancers. Cancer Research. doi:10.1158/0008-5472.can-17-1990</a></li> <li><a href="https://www.nature.com/articles/s41598-018-31496-1" target="_blank" rel="noopener noreferrer">Wylezich C, Papa A, Beer M, Höper D. A Versatile Sample Processing Workflow for Metagenomic Pathogen Detection. Sci Rep. 2018;8(1):13108.</a></li> </ol> </div> </div> </div></div></div></div> </div> </div> </div> </div> </address></address> <style> .mp-redirect-yes.mpstoreswitcher-select a { color: #757575; text-decoration: none; } .mp-redirect-yes.mpstoreswitcher-select a:hover { color: #FFFFFF; } @media screen and (max-width: 768px) { .mp-redirect-yes.mpstoreswitcher-select { margin-top: 5px; } } </style> </div></div></main><div class="page-bottom"><div class="content"><div class="widget block block-static-block"> <p><script> (function(d,b,a,s,e){ var t = b.createElement(a), fs = b.getElementsByTagName(a)[0]; t.async=1; t.id=e; t.src=s; fs.parentNode.insertBefore(t, fs); }) (window,document,'script','https://tag.demandbase.com/4f14a8c59acde313.min.js','demandbase_js_lib'); </script> <script type="text/javascript"> piAId = '420802'; piCId = '161336'; piHostname = 'pi.pardot.com'; (function() { function async_load(){ var s = document.createElement('script'); s.type = 'text/javascript'; s.src = ('https:' == document.location.protocol ? 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