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style="background-image:url(https://www.thermofisher.com/blog/life-in-the-lab/wp-content/uploads/sites/10/2022/08/AdobeStock_233726552-scaled.jpeg)"> </section> <div class="site-inner"><div class="content-sidebar-wrap"><main class="content"><article class="post-1589 post type-post status-publish format-standard has-post-thumbnail category-biotech category-cancer-research category-cell-gene-therapy category-connect-to-science category-general category-stem-cell-research tag-car-t tag-cell-therapy entry" aria-label="What Do Cells Share in Therapy?"><header class="entry-header"><h1 class="entry-title">What Do Cells Share in Therapy?</h1> <span class="post-info-author"> By <a href="https://www.thermofisher.com/blog/author/lifeinthelabstaff/" title="Posts by Life in the Lab Staff" rel="author">Life in the Lab Staff</a> </span> <span class="post-info-date"> 08.23.2022 </span> </header><div class="entry-content"><p>From its first trial in 1982, the life sciences community has had high hopes for the potential of cell therapy. Repairing damaged tissues, treating once-intractable diseases; cell therapy poses profound possibilities for situations once thought to be beyond the reach of contemporary science.</p> <p>By 2001, scientists had employed cell therapy technology to not only create the first beating heart cells outside the human body,<sup>1</sup> but also to bestow upon the world a zoological marvel: Dolly, the world’s most famous sheep.<sup>2 </sup>The burgeoning field’s capacity to shift the landscape of health technologies seems boundless.</p> <p>However, cell therapy isn’t without its practical challenges. By educating ourselves on how cell therapy works, and identifying potential solutions to its obstacles, we can all work towards empowering cell therapy technology to meet its potential.</p> <h2><span lang="EN-US" xml:lang="EN-US" data-contrast="auto">What is Cell Therapy?</span></h2> <p> </p> <p><iframe title="A Cell Therapy Journey from Vein to Vein" width="760" height="428" src="https://www.youtube.com/embed/7IlIzXVc2lg?feature=oembed" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe></p> <p><span class="TextRun SCXW219062476 BCX0" lang="EN-US" xml:lang="EN-US" data-contrast="auto"><span class="NormalTextRun SCXW219062476 BCX0">Cell therapy is the process of cultivating viable cells </span><span class="NormalTextRun SCXW219062476 BCX0">and introducing them (through injection, grafting, or implanting) into a cellular environment</span><span class="NormalTextRun SCXW219062476 BCX0"> for therapeutic treatment. By replacing lost or damaged material,</span><span class="NormalTextRun SCXW219062476 BCX0"> viable cells </span><span class="NormalTextRun SCXW219062476 BCX0">may present therapeutic relief in the treatment of a disease, neurological disorder, or major accident. </span></span><span class="EOP SCXW219062476 BCX0" data-ccp-props="{}"> </span></p> <h2><span class="TextRun SCXW52179895 BCX0" lang="EN-US" xml:lang="EN-US" data-contrast="auto"><span class="NormalTextRun SCXW52179895 BCX0">How does it work? </span></span><span class="EOP SCXW52179895 BCX0" data-ccp-props="{}"> </span></h2> <p><span data-contrast="auto">In the 19</span><span data-contrast="auto">th</span><span data-contrast="auto"> century, physiologist Charles-Édouard Brown-Séquard<sup>3</sup> sought to reverse the symptoms of aging with injections of animal cells.</span><span data-contrast="auto">iii</span><span data-contrast="auto"> Modern cell therapy has developed from a similar principle – treatment from the introduction of intentionally cultivated cells – but with a more precise scope. </span><span data-ccp-props="{}"> </span></p> <h3><span data-contrast="auto">Allotransplantation</span><span data-ccp-props="{}"> </span></h3> <p><span data-contrast="auto">If you’ve ever donated blood, you may have taken part in allotransplantation. In this method, genetic material – cells, muscles, organs – is taken from one organism and transplanted into a non-genetically identical member of the same species. For humans, this is often seen in blood transfusions and organ implants. </span><span data-ccp-props="{}"> </span></p> <p><span data-contrast="auto">The transplanted material, known as an allograft or allogeneic transplant, may trigger immunosuppressive responses from the body. For this reason, commercially available products such as </span><a href="https://www.thermofisher.com/us/en/home/clinical/cell-gene-therapy/cell-gene-therapy-learning-center/cell-gene-therapy-resource-library/cell-therapy-handbook/overview-cell-isolation-engineering-expansion.html?icid=bid_cbu_sbu_r01_co_CP1450_PJT9002_bid11111_0so_blg_op_awa_kt_s00_celltherapy1"><span data-contrast="none">allogeneic CAR T cell therapies</span></a><span data-contrast="auto">, require conscientious gene editing technique to minimize the risks of immunosuppressive rejection. </span><span data-ccp-props="{}"> </span></p> <p><span data-contrast="auto">Allogeneic CAR T cell therapies utilize a chimeric antigen receptor (CAR) engineered to be expressed on immune cells such as T and NK cells. This allows for the targeting of specific cancerous cells. </span><span data-ccp-props="{}"> </span></p> <p><span data-contrast="auto">Allogeneic CAR T cells, as a singular product that can be used for multiple patients, offer a wider range of application than autologous CAR T cells. By editing multiple genes, allogeneic CAR T cell products can be configured for multiple cancer targets. </span><span data-ccp-props="{}"> </span></p> <p><span data-ccp-props="{}"> </span><span data-contrast="auto">These products offer a treatment that combines the accessibility of a mass-produced, off-the-shelf product with the focused implantation needed to treat individuals. </span><span data-ccp-props="{}"> </span></p> <h3><span data-contrast="auto">Autotransplantation</span><span data-ccp-props="{}"> </span><span data-ccp-props="{}"> </span></h3> <p><span data-contrast="auto">This method is a little more personal. In autotransplantation, cells from one part of an individual organism are transplanted to a different part of that same organism. A very common example of this process happens in hospitals everyday when healthcare workers collect blood from someone expecting a surgery or other major medical intervention. </span><span data-ccp-props="{}"> </span><span data-ccp-props="{}"> </span></p> <p><span data-contrast="auto">The blood, if correctly stored, is easily re-introduced back to the individual, aiding in their recuperation with an influx of blood that poses minimal risk of rejection.</span><span data-ccp-props="{}"> </span><span data-ccp-props="{}"> </span></p> <p><span data-contrast="auto">The ease with which </span><a href="https://www.thermofisher.com/us/en/home/global/forms/car-t-cell-manufacturing-white-paper.html?icid=bid_cbu_sbu_r01_co_CP1450_PJT9002_bid11111_0so_blg_op_awa_kt_s00_celltherapy7"><span data-contrast="none">autologous CAR T cell therapies</span></a><span data-contrast="auto"> bypass potentially fatal immunosuppressive responses present a clear advantage for this method in cases when applicable. However, the conditions that afford autologous that advantage also pose technical limitations to its scalability. </span></p> <h2><span data-contrast="auto">What Would Cells in Therapy Share if they Could?</span><span data-ccp-props="{}"> </span></h2> <p><span data-ccp-props="{}"> </span><span data-contrast="auto">Cell therapies are beset by several practical considerations that pose limits on more global applications of the technology. </span><span data-ccp-props="{}"> </span></p> <p><span data-contrast="auto">The intended outcomes of cell therapy – whether it’s treating cancer or rehabilitating an athletic injury – aren’t accomplished with one or two or even ten good cells. It takes many, many cells, transplanted over the course of potentially many treatments, for long-term therapeutic benefit. </span><span data-ccp-props="{}"> </span></p> <p><span data-contrast="auto">This can be especially problematic in situations where autologous cells are required: an organism only produces so many cells over the course of their lifetime. Viable cells intended for treatment must be produced at a rate exponentially significant enough to fulfill the intended treatment. </span><span data-ccp-props="{}"> </span></p> <p><span data-ccp-props="{}"> </span><span data-contrast="auto">Cultivating viable cells for transplant is time-consuming. In addition to the considerable labor of manual processes, there is also the time-intensive task of keeping the product compliant with ever-changing clinical safety standards.</span><span data-ccp-props="{}"> </span></p> <p><span data-ccp-props="{}"> </span><span data-contrast="auto">With these scalability setbacks, the challenge of translating R&D into a commercially feasible product persists. </span><span data-ccp-props="{}"> </span></p> <h2><span data-contrast="auto">Innovation Isn’t a Hard Cell</span></h2> <p> </p> <p><iframe title="C2S Innovation Insights: the secret to a successful cell therapy manufacturing workflow" width="760" height="428" src="https://www.youtube.com/embed/AACgCxR9hPY?feature=oembed" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe></p> <p><span data-contrast="auto">We believe that scalability Is crucial to widespread application of cell therapy technologies. To better realize solutions to this global challenge, Thermo Fisher Scientific is applying next-gen instruments that ensure sterile conditions and optimize the pace of work to elevate cell therapy processes.</span></p> <p> </p> <h3><span data-contrast="auto">Instrumental to your Cell Therapy Success:</span><span data-ccp-props="{}"> </span><span data-ccp-props="{}"> </span></h3> <ul> <li><a href="https://www.thermofisher.com/us/en/home/clinical/cell-gene-therapy/cell-therapy/cell-therapy-manufacturing-solutions/xenon-electroporation-system.html?icid=bid_cbu_sbu_r01_co_CP1450_PJT9002_bid11111_0so_blg_op_awa_kt_s00_celltherapy2"><span data-contrast="none">CTS Xenon Electroporation</span></a><span data-ccp-props="{}"> </span></li> <li><a href="https://www.thermofisher.com/us/en/home/clinical/cell-gene-therapy/cell-therapy/cell-therapy-manufacturing-solutions/rotea-counterflow-centrifugation-system.html?icid=bid_cbu_sbu_r01_co_CP1450_PJT9002_bid11111_0so_blg_op_awa_kt_s00_celltherapy3"><span data-contrast="none">CTS Rotea Counterflow Centrifugation System</span></a><span data-ccp-props="{}"> </span></li> <li><a href="https://www.thermofisher.com/us/en/home/life-science/genome-editing/geneart-crispr.html?ef_id=CjwKCAjw_b6WBhAQEiwAp4HyIMIYENhseSjAk4TuY4l-17IqRE0gs-3Q6UfkWQJ3w3IeW8TmQDjQfhoCIbYQAvD_BwE:G:s&s_kwcid=AL!3652!3!507435096926!p!!g!!true%20cut%20cas9&cid=bid_clb_gme_r01_co_cp0000_pjt0000_bid00000_0se_gaw_bt_pur_con&gclid=CjwKCAjw_b6WBhAQEiwAp4HyIMIYENhseSjAk4TuY4l-17IqRE0gs-3Q6UfkWQJ3w3IeW8TmQDjQfhoCIbYQAvD_BwE?icid=bid_cbu_sbu_r01_co_CP1450_PJT9002_bid11111_0so_blg_op_awa_kt_s00_celltherapy4"><span data-contrast="none">CTS TrueCut Cas9 v2 Protein</span></a></li> <li><a href="https://www.thermofisher.com/us/en/home/life-science/cell-analysis/flow-cytometry/flow-cytometers/attune-nxt-flow-cytometer/models/cytpix.html?icid=bid_cbu_sbu_r01_co_CP1450_PJT9002_bid11111_0so_blg_op_awa_kt_s00_celltherapy5"><span data-contrast="none">Attune Cytpix Flow Cytometer</span></a><span data-ccp-props="{}"> </span></li> </ul> <p><span data-contrast="auto">As every scientific concept must thread the needle of practical application, the advancement of cell therapy technologies must overcome its “real-world” challenges to effectively realize their mainstream capabilities. When the scalability of cell therapy technologies is increased, so is the overall potential for all those who may utilize it to reach their therapeutic aims.</span><span data-ccp-props="{}"> </span></p> <p><span data-contrast="auto">To connect with Thermo Fisher Scientific’s innovations in cell therapy, check out </span><a href="https://www.thermofisher.com/us/en/home/clinical/cell-gene-therapy/cell-therapy.html?icid=bid_cbu_sbu_r01_co_CP1450_PJT9002_bid11111_0so_blg_op_awa_kt_s00_celltherapy6"><span data-contrast="none">our Cell Therapy Solutions homepage</span></a><span data-contrast="auto">. </span><span data-ccp-props="{}"> </span></p> <p> </p> <p><strong><em>Want to read more stories like this? <a href="https://www.thermofisher.com/us/en/home/global/forms/connect-science-subscribe-giveaway.html">Subscribe to Connect to Science</a>, your portal for life science news. </em></strong></p> <p> </p> <h3>References</h3> <p><sup>1 </sup>Kocher AA et. al. “Stem cells and cardiac regeneration.” Transpl Int. 2007 Sep;20(9):731-46. doi: 10.1111/j.1432-2277.2007.00493.x.</p> <p><sup>2 </sup><a href="https://www.youtube.com/watch?v=dhvgb0H7jko">Gibco® Sera-part of important breakthroughs for more than 50 yrs.</a></p> <p><sup>3 </sup> Lefrere JJ and Berche P. “La thérapeutique du docteur Brown-Séquard” Annales d’endocrinologie. 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