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<!DOCTYPE html><html><head><title>Dark Art of Blood Cultures - Google Books</title><link rel="stylesheet" href="/books/css/_ff77d0f0508c7e0bad470e7d6b7f28fa/kl_viewport_kennedy_full_bundle.css" type="text/css" /><link rel="stylesheet"href="https://fonts.googleapis.com/css2?family=Product+Sans:wght@400"><script src="/books/javascript/v2_ff77d0f0508c7e0bad470e7d6b7f28fa__en.js"></script><script>_OC_Hooks = ["_OC_Page", "_OC_SearchReload", "_OC_TocReload", "_OC_EmptyFunc", "_OC_SearchPage", "_OC_QuotePage" ];for (var _OC_i = 0; _OC_i < _OC_Hooks.length; _OC_i++) {eval("var " + _OC_Hooks[_OC_i] + ";");}function _OC_InitHooks () {for (var i = 0; i < _OC_Hooks.length; i++) {var func = arguments[i];eval( _OC_Hooks[i] + " = func;");}}</script><link rel="canonical" href="https://books.google.com/books/about/Dark_Art_of_Blood_Cultures.html?id=_gjMDwAAQBAJ"/><meta property="og:url" content="https://books.google.com/books/about/Dark_Art_of_Blood_Cultures.html?id=_gjMDwAAQBAJ"/><meta name="title" content="Dark Art of Blood Cultures"/><meta name="description" content="In the clinical microbiology laboratory, blood is a critical diagnostic sample that, in the majority of cases is sterile (or is it?). However, when microbes gain access to and multiply in the bloodstream, it can result in life-threatening illness including sepsis. Mortality rates from bloodstream infection and sepsis range from 25% to 80%, killing millions of people annually. Blood cultures are a vital technology used in the microbiology laboratory to isolate and identify microbes and predict their response to antimicrobial therapy. The Dark Art of Blood Cultures, edited by Wm. Michael Dunne, Jr., and Carey-Ann D. Burnham, surveys the entire field of blood culture technology, providing valuable information about every phase of the process, from drawing samples to culture methods to processing positive cultures. The Dark Art of Blood Cultures is organized around several major topics. History of blood culture methods. Details the timeline of blood culture methods from manual through automated and describes the technological development of the leading automated blood culture systems (Bactec, BacT/Alert, and VersaTREK). Manual and automated blood culture methods. Critiques manual and automated methods for setting up blood cultures for adult and pediatric patients. Detection of pathogens directly from blood specimens. Describes currently available CE marked and FDA-cleared commercial tests using both phenotypic and genotypic markers, including their strengths and limitations. The workflow of culturing blood. Includes best practices from specimen collection to culture system verification, processing positive cultures for microbe identification and antibiotic susceptibility determination, along with the epidemiology of positive blood cultures and the value of postmortem blood cultures. Microorganisms in the blood. Examines the concept of a blood microbiome in healthy and diseased individuals. The Dark Art of Blood Cultures is a resource that clinicians, laboratorians, lab directors, and hospital administrators will find engaging and extremely useful. If you are looking for online access to the latest clinical microbiology content, please visit www.wiley.com/learn/clinmicronow. 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Michael Dunne, Jr., Carey-Ann D. Burnham","additional_info":{"[JsonBookInfo]":{"BuyLinks":[{"Seller":"Wiley.com","Url":"http://www.wiley.com/remtitle.cgi?ISBN=9781683673064","TrackingUrl":"/url?client=ca-google-print\u0026format=googleprint\u0026num=0\u0026id=_gjMDwAAQBAJ\u0026q=http://www.wiley.com/remtitle.cgi%3FISBN%3D9781683673064\u0026usg=AOvVaw0YqGpKUxnd0Y0KGZiASNj-","IsPublisher":true},{"Seller":"Amazon.com","Url":"http://www.amazon.com/gp/search?index=books\u0026linkCode=qs\u0026keywords=9781683673064","TrackingUrl":"/url?client=ca-google-print\u0026format=googleprint\u0026num=0\u0026id=_gjMDwAAQBAJ\u0026q=http://www.amazon.com/gp/search%3Findex%3Dbooks%26linkCode%3Dqs%26keywords%3D9781683673064\u0026usg=AOvVaw2eqf8YIAZ5hcb0LZntLW0H"},{"Seller":"MPH","Url":"http://www.mphonline.com/books/nsearch.aspx?do=detail\u0026pcode=9781683673064","TrackingUrl":"/url?client=ca-google-print\u0026format=googleprint\u0026num=0\u0026id=_gjMDwAAQBAJ\u0026q=http://www.mphonline.com/books/nsearch.aspx%3Fdo%3Ddetail%26pcode%3D9781683673064\u0026usg=AOvVaw17urD9RUXATLTIz_wabwHi"},{"Seller":"Popular","Url":"https://www.popular.com.sg/catalogsearch/result/?q=9781683673064","TrackingUrl":"/url?client=ca-google-print\u0026format=googleprint\u0026num=0\u0026id=_gjMDwAAQBAJ\u0026q=https://www.popular.com.sg/catalogsearch/result/%3Fq%3D9781683673064\u0026usg=AOvVaw3XRp88VaJY5tzfXzvE5h0J"}],"AboutUrl":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ","PreviewUrl":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ","allowed_syndication_flags":{"allow_disabling_chrome":true},"online_access":{"url":"https://play.google.com/store/books/details?id=_gjMDwAAQBAJ\u0026rdid=book-_gjMDwAAQBAJ\u0026rdot=1","price":"SGD 81.99","list_price":"SGD 81.99"},"TocLine":[{"Title":"Contributors ","Pid":"PT9","PgNum":"","Order":9},{"Title":"Conventional Blood Culture Methods ","Pid":"PT44","PgNum":"","Order":44},{"Title":"LysisCentrifugation Methods of Blood Culture ","Pid":"PT70","PgNum":"","Order":70},{"Title":"Bactec Blood Culture Systems ","Pid":"PT99","PgNum":"","Order":99},{"Title":"Automation at Last in the Black ","Pid":"PT134","PgNum":"","Order":134},{"Title":"TREK Blood Culture Systems ","Pid":"PT172","PgNum":"","Order":172},{"Title":"Molecular Methods for Detection of Pathogens Directly from ","Pid":"PT207","PgNum":"","Order":207},{"Title":"Pediatric Blood Cultures ","Pid":"PT225","PgNum":"","Order":225},{"Title":"Epidemiology of Bloodstream Infections ","Pid":"PT243","PgNum":"","Order":243},{"Title":"Best Practices in Blood Cultures ","Pid":"PT271","PgNum":"","Order":271},{"Title":"Processing Positive Cultures ","Pid":"PT305","PgNum":"","Order":305},{"Title":"Fungal and Mycobacterial Blood Cultures ","Pid":"PT361","PgNum":"","Order":361},{"Title":"Postmortem Blood Culture ","Pid":"PT431","PgNum":"","Order":431}]}},"table_of_contents_page_id":"PT7","max_resolution_image_width":1280,"max_resolution_image_height":1921,"num_toc_pages":2,"quality_info":"We have no quality information about this book.","volume_id":"_gjMDwAAQBAJ","permission_info":"Pages displayed by permission of \u003ca class=link_aux href=\"https://books.google.com.sg/url?id=_gjMDwAAQBAJ\u0026pg=PT207\u0026q=http://www.wiley.com\u0026linkid=1\u0026usg=AOvVaw0U54npQDgoTHFbpmXE5MoR\u0026source=gbs_pub_info_r\"\u003eJohn Wiley \u0026amp; Sons\u003c/a\u003e","is_ebook":true,"volumeresult":{"has_flowing_text":true,"has_scanned_text":true,"can_download_pdf":false,"can_download_epub":false,"is_pdf_drm_enabled":false,"is_epub_drm_enabled":false},"publisher":"John Wiley \u0026 Sons","publication_date":"2017.09.01","subject":"Medical","sample_url":"https://play.google.com/books/reader?id=_gjMDwAAQBAJ\u0026source=gbs_vpt_hover","synposis":"In the clinical microbiology laboratory, blood is a critical diagnostic sample that, in the majority of cases is sterile (or is it?). However, when microbes gain access to and multiply in the bloodstream, it can result in life-threatening illness including sepsis. Mortality rates from bloodstream infection and sepsis range from 25% to 80%, killing millions of people annually. Blood cultures are a vital technology used in the microbiology laboratory to isolate and identify microbes and predict their response to antimicrobial therapy. The Dark Art of Blood Cultures, edited by Wm. Michael Dunne, Jr., and Carey-Ann D. Burnham, surveys the entire field of blood culture technology, providing valuable information about every phase of the process, from drawing samples to culture methods to processing positive cultures. The Dark Art of Blood Cultures is organized around several major topics. History of blood culture methods. Details the timeline of blood culture methods from manual through automated and describes the technological development of the leading automated blood culture systems (Bactec, BacT/Alert, and VersaTREK). Manual and automated blood culture methods. Critiques manual and automated methods for setting up blood cultures for adult and pediatric patients. Detection of pathogens directly from blood specimens. Describes currently available CE marked and FDA-cleared commercial tests using both phenotypic and genotypic markers, including their strengths and limitations. The workflow of culturing blood. Includes best practices from specimen collection to culture system verification, processing positive cultures for microbe identification and antibiotic susceptibility determination, along with the epidemiology of positive blood cultures and the value of postmortem blood cultures. Microorganisms in the blood. Examines the concept of a blood microbiome in healthy and diseased individuals. The Dark Art of Blood Cultures is a resource that clinicians, laboratorians, lab directors, and hospital administrators will find engaging and extremely useful. If you are looking for online access to the latest clinical microbiology content, please visit www.wiley.com/learn/clinmicronow. ","my_library_url":"https://www.google.com/accounts/Login?service=print\u0026continue=https://books.google.com.sg/books%3Fop%3Dlibrary\u0026hl=en","is_magazine":false,"is_public_domain":false,"last_page":{"pid":"PT480","order":480}},{"enableUserFeedbackUI":true,"pseudocontinuous":true,"is_cobrand":false,"sign_in_url":"https://www.google.com/accounts/Login?service=print\u0026continue=https://books.google.com.sg/books%3Fid%3D_gjMDwAAQBAJ%26q%3Dmolecular%26source%3Dgbs_word_cloud_r%26hl%3Den\u0026hl=en","isEntityPageViewport":false,"showViewportOnboarding":false,"showViewportPlainTextOnboarding":false},{"page":[{"pid":"PT207","flags":8,"order":207,"vq":"molecular"}]},null,{"number_of_results":42,"search_results":[{"page_id":"PT7","snippet_text":"... \u003cb\u003eMolecular\u003c/b\u003e Methods for Detection of Pathogens Directly from Blood Specimens Mark D. Gonzalez and Robert C. Jerris 8 Pediatric Blood Cultures Paula Revell and Christopher Doern 9 Epidemiology of Bloodstream Infections Allison R. McMullen\u0026nbsp;...","page_url":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ\u0026pg=PT7\u0026vq=molecular"},{"page_id":"PT15","snippet_text":"... \u003cb\u003eMolecular\u003c/b\u003e Microbiology , Pediatrics , and Medicine at Washington University School of Medicine in St. Louis from 2000–2011 and remains on the faculty there . During that time , he established an internationally - recognized training\u0026nbsp;...","page_url":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ\u0026pg=PT15\u0026vq=molecular"},{"page_id":"PT16","snippet_text":"... \u003cb\u003eMolecular\u003c/b\u003e Microbiology, and Pediatrics at Washington University in St. Louis School of Medicine. 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Several studies have shown that one can perform matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry from positive bioMérieux blood bottles after manual\u0026nbsp;..."},{"page_id":"PT152","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e assays have been developed to identify microorganisms directly from BacT/Alert bottles, including the Biofire Film Array (19), Nanosphere (20), peptide nucleic acid fluorescent in situ hybridization (PNA FISH) (21), and\u0026nbsp;..."},{"page_id":"PT156","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e amplification assays and MALDI-TOF mass spectrometry applications, in part, because of its tendency to absorb small \u003cb\u003emolecules\u003c/b\u003e required for chemical reactions and/or mechanical clogging. In response to these difficulties and\u0026nbsp;..."},{"page_id":"PT206","snippet_text":"... VersaTREK system compared to the Bactec MGIT 960 system for first - line drug susceptibility testing of Mycobacterium tuberculosis . J Clin Microbiol 50 : 488–491 . \u003cb\u003eMolecular\u003c/b\u003e Methods for Detection of Pathogens Directly from Blood Specimens."},{"page_id":"PT207","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e methods to provide rapid microorganism identification and resistance gene detection (e.g., mecA in Staphylococcus spp.) from positive blood culture broths. In a meta-analysis of these new methods, a significant reduction in\u0026nbsp;..."},{"page_id":"PT208","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e methods, which is largely because of the high viral loads present in those specimens. In contrast, the majority of septic patients have a paucity of bacteria or fungi per milliliter of blood, necessitating the need for larger\u0026nbsp;..."},{"page_id":"PT216","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e-based assays was less than that of blood cultures. ST detected organisms in six specimens (12%), of which ... \u003cb\u003emolecular\u003c/b\u003e assays as an add-on test. All systems suffer from the low volume of blood tested in the \u003cb\u003emolecular\u003c/b\u003e\u0026nbsp;..."},{"page_id":"PT217","snippet_text":"... \u003cb\u003eMolecular\u003c/b\u003e (Des Plaines, IL) is a CE-IVD, non-FDA-cleared test for direct pathogen detection from blood specimens. The system uses an innovative new technology that combines PCR with electrospray ionization mass spectrometry (ESI-MS) to\u0026nbsp;..."},{"page_id":"PT220","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e-test-positive specimens is an area in need of additional investigation. Similar to the conundrum of blood cultures positive with skin contaminants, the results from \u003cb\u003emolecular\u003c/b\u003e tests will need to be analyzed in the appropriate\u0026nbsp;..."},{"page_id":"PT221","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e methods come at an increased cost to laboratories and hospital systems. In the ever increasingly cost-conscious environment of medicine, studies are needed to justify the effectiveness of such methods from an economic and\u0026nbsp;..."},{"page_id":"PT223","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e detection of blood stream infection from a clinical perspective. BMC Infect Dis 16:314. 11. Wellinghausen N, Kochem AJ, Disqué C, Mühl H, Gebert S, Winter J, Matten J, Sakka SG. 2009. Diagnosis of bacteremia in whole-blood\u0026nbsp;..."},{"page_id":"PT295","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e methods for verification of results obtained with BacT / Alert Nonvent blood culture bottles . J Clin Microbiol 41 : 1266–1269 . 40. Kumar A , Haery C , Paladugu B , Kumar A , Symeoneides S , Taiberg L , Osman J , Trenholme G\u0026nbsp;..."},{"page_id":"PT318","snippet_text":"... \u003cb\u003eMolecular\u003c/b\u003e Detection from Positive Blood Cultures In recent years, several \u003cb\u003emolecular\u003c/b\u003e assays have come to market that reduce the TAT for positive blood cultures. Unlike MALDI- TOF MS, these assays are FDA cleared, allowing for easier\u0026nbsp;..."},{"page_id":"PT324","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e panels for positive blood culture evaluation. However, the contribution of the assay results on patient care may depend upon each hospital\u0026#39;s prevalence of bacteremia caused by organisms that identification changes empirical\u0026nbsp;..."},{"page_id":"PT331","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e methods, but are less expensive options for testing than traditional culture alone that can improve patient care. Immunochromatographic assays Many \u003cb\u003emolecular\u003c/b\u003e tests require moderate- or high-complexity laboratories with well-\u0026nbsp;..."},{"page_id":"PT332","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e tests require moderate- or high-complexity laboratories with well-trained laboratory professionals to ensure that contamination does not occur during the performance of the test. In addition, most \u003cb\u003emolecular\u003c/b\u003e assays can\u0026nbsp;..."},{"page_id":"PT334","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e panels report genotypic resistance markers, but these have limited targets and do not provide a complete susceptibility profile for optimal patient treatment (current exception is Accelerate diagnostics assay). Laboratories\u0026nbsp;..."},{"page_id":"PT339","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e composition of a specimen compared with a reference library of known spectra. Evaluation of Raman and FTIR spectroscopy on microcolonies from positive blood culture bottles found high accuracy for both Raman, 92.2%, and FTIR\u0026nbsp;..."},{"page_id":"PT343","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e device can be used for all positive bottles. This is best done using a large panel that covers the most common blood pathogens. (C) To reduce cost or improve patient care, a multifaceted approach can be implemented where the\u0026nbsp;..."},{"page_id":"PT351","snippet_text":"... blood culture broths by use of the Verigene Gram - negative blood culture multiplex microarray - based \u003cb\u003emolecular\u003c/b\u003e assay . J Clin Microbiol 53 : 2460–2472 . 48 . 49 . 50 . 51 . Buchan BW. 47 . 55 . 62 . 63 . Faron ML CC ,"},{"page_id":"PT353","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e assays for identification of causative organisms in bacterial bloodstream infections . Eur J Clin Microbiol Infect Dis 34 : 487–496 . 57 . Messacar K , Hurst AL , Child J , Campbell K , Palmer C , Hamilton S , Dowell E\u0026nbsp;..."},{"page_id":"PT360","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e rapid diagnostic testing on clinical outcomes in bloodstream infections : a systematic review and meta - analysis . Clin Infect Dis 64 : 15–23 . Fungal and Mycobacterial Blood Cultures Robert S. Liao1 and William."},{"page_id":"PT388","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e diagnostics could enable clinical microbiology laboratories to quickly identify many species of mycobacteria. PCR assays, such as the Cepheid Xpert MTB/RIF assay (Sunnyvale, CA) and a multiplex MAC PCR (109) on primary\u0026nbsp;..."},{"page_id":"PT406","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e methods, these data only reflect the presence of DNA sequences derived from microorganisms in the specimen and not the microorganisms themselves. Thus, microbial DNA sequences in blood could correspond to freely circulating\u0026nbsp;..."},{"page_id":"PT415","snippet_text":"... to the cellular damage caused by reactive oxygen and nitrogen \u003cb\u003emolecules\u003c/b\u003e and constant production of cytotoxic mediators by activated glial cells can result in damage and death of neurons ( 51 , 52 ) . The finding that."},{"page_id":"PT427","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e amplification of highly substoichiometric levels of bacterial lipopolysaccharide. JR Soc Interface 13:20160539. 51. Park J, Min JS, Kim B, Chae UB, Yun JW, Choi MS, Kong IK, Chang KT, Lee DS. 2015. Mitochondrial ROS govern the\u0026nbsp;..."},{"page_id":"PT441","snippet_text":"... classification of results in these cases are not routinely used, it remains necessary to carefully evaluate and interpret microbiological findings from autopsy. \u003cb\u003eMolecular\u003c/b\u003e Testing for Blood Pathogens The development of \u003cb\u003emolecular\u003c/b\u003e testing."},{"page_id":"PT449","snippet_text":"... \u003cb\u003eMolecular\u003c/b\u003e , IRIDICA BAC BSI assay ( IRIDICA ) , 145–146 Abiotrophia spp . , blood culture - negative endocarditis ( BCNE ) , 167 Acid - fact bacilli ( AFB ) , isolation of , 49–52 Acidovorax defluvii , 281 Acinetobacter , 27 , 282 , 289\u0026nbsp;...","page_url":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ\u0026pg=PT449\u0026vq=molecular"},{"page_id":"PT451","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e technologies , 98 evolution of , and VIRTUO systems , 96–98 first prototype , 91 fungal blood culture , 248 , 250–251 glass to plastic , 100 history of , 85–88 initial commercial success , 94–95 mechanism of growth detection\u0026nbsp;...","page_url":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ\u0026pg=PT451\u0026vq=molecular"},{"page_id":"PT463","snippet_text":"... 76 fungal blood culture , 249–250 fungemia , 246 isolation , 48 , 49 pediatric fungal blood cultures , 158 recovery from blood culture , 173 , 174 Human Microbiome Project , 277 I iCubate system , \u003cb\u003emolecular\u003c/b\u003e detection , 218 , 222.","page_url":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ\u0026pg=PT463\u0026vq=molecular"},{"page_id":"PT464","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e detection , 218 , 222 Incubation , blood cultures , 22–23 Integrated Quality Laboratory Services ( IQLS ) , 28 Invasive mold infections , pediatric , 170–171 IRIDICA BAC BSI assay pathogen detection , 145–146 test parameters\u0026nbsp;...","page_url":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ\u0026pg=PT464\u0026vq=molecular"},{"page_id":"PT467","snippet_text":"... \u003cb\u003eMolecular\u003c/b\u003e detection FilmArray BCID panel , 220–222 GenMark ePlex , 222 iCubate iC - GPC , 218 , 222 positive blood cultures , 216–217 , 220–222 rapid identification assays , 218–219 Verigene BC - GN and BC - GP assay , 216–217 , 220\u0026nbsp;...","page_url":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ\u0026pg=PT467\u0026vq=molecular"},{"page_id":"PT470","snippet_text":"... \u003cb\u003eMolecular\u003c/b\u003e methods for pathogen detection Pediatric bacteremia , epidemiology of , 151–153 Pediatric blood cultures anaerobic blood culture media , 156–157 antibiotic absorbing substances , 156 blood culture media , 155–157 epidemiology\u0026nbsp;...","page_url":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ\u0026pg=PT470\u0026vq=molecular"},{"page_id":"PT471","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e testing for blood pathogens , 304–305 relevance of microbiology testing , 206 Prevotella spp . , 164 Processing positive cultures , 203 , 233–236 alternative approaches , 226–229 automated AST systems , 229 direct plating to\u0026nbsp;...","page_url":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ\u0026pg=PT471\u0026vq=molecular"},{"page_id":"PT472","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e detection , 216–217 , 220–222 MRSA - specific PCR assays , 225–226 peptide nucleic acid fluorescent in situ hybridization ( PNA FISH ) , 223–225 rapid diagnostics , 211–229 staining techniques and subculture , 208–209 , 211\u0026nbsp;...","page_url":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ\u0026pg=PT472\u0026vq=molecular"},{"page_id":"PT473","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e detection from positive blood cultures , 216–222 MRSA - specific PCR assays , 225–226 peptide nucleic acid fluorescent in situ hybridization ( PNA FISH ) , 223–225 see also Processing positive cultures Reller , Barth , 91.","page_url":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ\u0026pg=PT473\u0026vq=molecular"},{"page_id":"PT474","snippet_text":"... \u003cb\u003emolecular\u003c/b\u003e methods , 137–138 , 147–148 see also \u003cb\u003eMolecular\u003c/b\u003e methods for pathogen detection SepsiTest ( ST ) BLAST tool , 143 pathogen detection , 142–143 test parameter , 139 Septi - Chek blood culture system , 25–27 , 26 Pediatric\u0026nbsp;...","page_url":"https://books.google.com.sg/books?id=_gjMDwAAQBAJ\u0026pg=PT474\u0026vq=molecular"}],"search_query_escaped":"molecular"},{});</script></div></div></div><script>(function() {var href = window.location.href;if (href.indexOf('?') !== -1) {var parameters = href.split('?')[1].split('&');for (var i = 0; i < parameters.length; i++) {var param = parameters[i].split('=');if (param[0] == 'focus') {var elem = document.getElementById(param[1]);if (elem) {elem.focus();}}}}})();</script>