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as a Method * define function as object methods * @type {Object} */ window.digitalData = { user: {}, page: {}, merchandising: {}, getQueryParam : function (param) { var index, key, value, hash; var hashes = window.location.href.slice(window.location.href.indexOf('?') + 1).split('&'); for (index = 0; index < hashes.length; index++) { hash = hashes[index].split('='); key = hash[0]; value = hash[1]; if (key.toUpperCase() === param.toUpperCase()) return value; } return ''; }, getValOnce: function (value, cookieName, expiry, timeUnit) { var currentDate = new Date; var currentCookieValue; // timeUnit of 'm' is minutes represented in milliseconds // thus 86400000 = 24 hours = 1 day var interval = timeUnit == 'm' ? 60000 : 86400000; cookieName = cookieName ? cookieName : 'digitaldata_gvo'; value = value ? value : ''; expiry = expiry ? expiry : 0; currentCookieValue = this.getCookie(cookieName); if (value) { currentDate.setTime(currentDate.getTime() + expiry * interval); this.setCookie(cookieName, value, expiry == 0 ? 0 : currentDate); } return value == currentCookieValue ? '' : value; }, getCookie: function (key) { var result = ""; var cookies = document.cookie ? document.cookie.split('; ') : []; for (var i = 0; i < cookies.length; i++) { var parts = cookies[i].split('='); var name = decodeURIComponent(parts.shift()); var cookie = parts ? parts.join('=') : []; // Prevent storing or parsing of a cookie that we couldn't decode. if (cookie && key === name) { // removes surrounding quotes if (cookie.indexOf('"') === 0) { // This is a quoted cookie as according to RFC2068, unescape... cookie = cookie.slice(1, -1).replace(/\\"/g, '"').replace(/\\\\/g, '\\'); } // parse utf8 characters try { cookie = decodeURIComponent(cookie); } catch (e) {} result = cookie; // we found it, don't bother looking at other cookies break; } } return result; }, setCookie : function(name, value, numDays){ if ( !numDays ) numDays = 31; var expires = new Date(); expires.setTime(expires.getTime() + (1000 * 60 * 60 * 24 * numDays)); document.cookie = name+ '=' + escape(value) + '; path=/; expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); }, // generic function to set page attributes setPageAttribute: function (key, value) { this.page[key] = value; }, // display digitalData object displayDigitalData: function () { }, // specific function to add an object to digitalData addDigitDataObject: function (key, object) { this[key] = object; }, addChildObjectAttribute: function(child, key, value){ this[child][key] = value; }, initUserData: function(){ var gigyaCookie = this.getCookie("identity_uid"); this.user.gigyaId = ""; this.user.loginStatus = "anonymous"; if( gigyaCookie != ""){ this.user.gigyaId = gigyaCookie; this.user.loginStatus = "logged in"; } }, /** * Check if init has been initiated once * @type {Boolean} */ merchandisingDataInitted: false, initMerchandisingData: function () { // read cid and icid value from session cookie this.merchandising.icid = this.getCookie('s_ev21_tc'); this.merchandising.cid = this.getCookie('s_cmp_tc'); // EMID value this.merchandising.emid = this.getQueryParam("emid"); // referrer by domain if (typeof document.referrer !== 'undefined' && document.referrer.length > 0) { var masterDomain = this.getMasterDomain(); var refReg = document.referrer; var referrerDomain = refReg.match(/:\/\/([^/]+)\//); if (referrerDomain !== null && referrerDomain[1].indexOf(masterDomain) == -1) { this.merchandising.referrer = referrerDomain; } } document.addEventListener('registerMerchandisingData', function (event) { for (var key in event.detail) { console.log("Listener data copied", key, event.detail[key] ); digitalData.merchandising[key] = event.detail[key]; } // Map some analytics attributes to merchandising session attributes digitalData.merchandising.sessionId = digitalData.merchandising.analyticsVisitorID; digitalData.merchandising.s_vi_low = digitalData.merchandising.analyticsVisitorIDLow; digitalData.merchandising.s_vi_high = digitalData.merchandising.analyticsVisitorIDHigh; console.log("digitalData: Captured registerMerchandisingData event"); digitalData.merchandisingDataInitted = true; // Call emailCampaignRecord if the digitalDataAnalyticsListener object is loaded if (typeof digitalDataAnalyticsListener != "undefined") { console.log("digitalData: Calling email campaign record"); digitalDataAnalyticsListener.emailCampaignRecord(); } }); }, formSubmitEvent: function () { var formId = this.getQueryParam("form-submit-id"); if ((formId) && formId !== "" && formId !== null) { var eventDetail = { detail: { form: { id: formId } } }; var formSubmitEvent = new CustomEvent('formSubmit', eventDetail); console.log('form submit event started'); document.dispatchEvent(formSubmitEvent); console.log('form submit event fired', eventDetail); //flush grepToken from Session Storage sessionStorage.removeItem('grepTokenSO'); } }, modalVideoEvent: function (videoId) { if (((videoId) && videoId !== "" && videoId !== null)) { var eventDetail = { detail: { video: { playerId: videoId } } }; var modalVideoEvent = new CustomEvent('videoPlayerLoaded', eventDetail); document.dispatchEvent(modalVideoEvent); } }, customClickEvent: function (clickDetail) { if (((clickDetail) && clickDetail !== "" && clickDetail !== null)) { var eventDetail = { detail: { CustomLink: { linkName: clickDetail } } } var customClickEvent = new CustomEvent('customLinkClicks', eventDetail); document.dispatchEvent(customClickEvent); } } } })(); window.digitalData.init(); </script>  <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_25"); </script> <script type="text/javascript"> var localizedStrings = { "ACCEPTANCE": "Acceptance of Terms of Use", "BTN_AGREE": "Agree", "BTN_DISAGREE": "Disagree", "PROCESSING_MESSAGE" :"processing ... please wait ...", "ADD_TO_CART_QUANTITY_ERROR":"You must provide a quantity to add an item to the cart", "ADD_TO_CART_VALID_QUANTITY_ERROR":"You must provide a valid quantity to add an item to the cart", "VIEW_CART" :"View cart & checkout", "VIEW_CONTACT_DETAIL" :"View Contact Details", "ADD_TO_CART_NOTIFICATION":"Add to Cart Notification", "UPDATE_CART" :"Update Cart", "PRINCIPAL_INVESTIGATOR":"Principal Investigator", "PRINCIPAL_INVESTIGATOR_TEAM_LEADER":"Principal Investigator or Team Leader", "PRINCIPAL_INVESTIGATOR_CHIEF":"Principal Investigator or Chief", "EMAIL_ERROR_1" :"Please Provide Collegues Email", "EMAIL_ERROR_2" :"Please enter a valid colleague email address", "EMAIL_ERROR_3" :"Please Provide Your Email", "EMAIL_ERROR_4" :"Please enter a valid email address", "ERROR_OCCURED_MESSAGE" :"The following errors occured", "NEXT" :"Next", "PREVIOUS" :"Prev", "IMAGE" :"Image", "SAVE_TO_LIST_ERROR_1" :"Please enter a list name.", "SAVE_TO_LIST_ERROR_2" :"Please enter a different list name that does not include the following: ?,/,\\,%,^,!", "MINICART_ERROR_1" :"Unable to connect to eCommerce system. 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onclick="window.THERMO_FISHER.pdp.inp.toggleImagesAdvVerificationPublishedFigures(event, this)"> <a class="link published-link animated-scroll " href="#image-gallery" onclick="window.THERMO_FISHER.pdp.inp.publishedFiguresHero(event, this)"> <svg class="svg-icon published-figures-icon"> <use href="#icon-published-figures"></use> </svg> 40 Published Figures </a> </div> <div class="details-hero"> <a class="link references-link animated-scroll " href="#references-component-id" onclick="window.THERMO_FISHER.pdp.inp.referencesHero(event, this)"> <svg class="svg-icon references-icon"> <use href="#icon-references"></use> </svg> 64 References </a> </div> <div class="details-hero"> <a class="btn hero-btn hero-view-other" href="/antibody/primary/target/cd9"> View all (61) CD9 antibodies </a> </div> </div> <div class="links-ab to-show-links-in-sm"> <div class="container"> <div class="link-container"> <a class="link-antibody" href="/order/genome-database/dataSheetPdf?producttype=antibody&productsubtype=antibody_primary&productId=10626D&version=Local" target="_blank" rel="nofollow"> <span> <svg class="svg-icon data-sheet-icon"> <use href="#icon-data-sheet"></use> </svg> </span> Datasheet </a> </div> <div class="link-container"> <a href="https://www.thermofisher.com/global/en/home/life-science/antibodies/antibodies-learning-center/antibody-protocols.html?icid=antibody-pdp-protocols" target="_blank" class="link-antibody"> <span> <svg class="svg-icon protocols-icon"> <use href="#icon-protocols"></use> </svg> </span> Protocols </a> </div> <div class="link-container"> <a href="/us/en/home/technical-resources/contact-us.10626D.html?supportType=TS" class="link-antibody"> <span> <svg class="svg-icon tech-support-icon"> <use href="#icon-tech-support"></use> </svg> </span> Tech Support </a> </div> </div> </div> <div class="cmrc-card-cnt" id="antibody_primary"> <div class="price-card-alignmnet" tf-product-order> <aside 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media-items="{'Antibody Testing Data':[],'Published Figures':[{'sku':'10626D','imageId':'1111619','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_30047_IJO-63-3-05550-g04.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_30047_IJO-63-3-05550-g04.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_30047_IJO-63-3-05550-g04.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_30047_IJO-63-3-05550-g04.jpg?time\u003d20240507','sortOrder':'1','description':'Morphology, size distribution, concentration and protein profiles of exosomes from HNSCC cell lines. Supernatants from HNSCC cell lines were collected and exosomes were isolated on mini-size exclusion chromatography columns. (A) Exosomes from UM-SCC-11B (11B), UM-SCC-14C (14C) and UM-SCC-22B (22B) show the typical vesicular shape and size in TEM images. Scale bar, 100 nm. (B) Western blotting was performed after loading 10 u g exosome preparation per lane and show the exosome markers TSG101, CD63 and CD9 and ApoA1 and Grp94 were used as purity control. (C) Nanoparticle tracking analysis was performed to detect median sizes and particle concentrations of the isolated particles. Representative pictures are shown of representative exosome preparations. HNSCC, head and neck squamous cell carcinoma.','shortDescription':'Morphology, size distribution, concentration and protein profiles of exosomes from HNSCC cell lines. Supernatants from HNSCC cell lines were collected and exosomes were isolated on mini-size exclusion chromatography columns. (A) Exosomes from UM-SCC-11B (11B), UM-SCC-14C (14C) and UM-SCC-22B (22B) show the typical vesicular shape and size in TEM images. Scale bar, 100 nm. (B) Western blotting was performed ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Sep 1, 2023, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'International journal of oncology','journalText':'International journal of oncology 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_30047_IJO-63-3-05550-g04.jpg','benchSciPubmedId':'37503786'},{'sku':'10626D','imageId':'1072153','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_27424_fphys-12-630933-g002.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_27424_fphys-12-630933-g002.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_27424_fphys-12-630933-g002.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_27424_fphys-12-630933-g002.jpg?time\u003d20240507','sortOrder':'2','description':'FIGURE 2 Particle population characteristics, zeta potential, and western blot analysis of cell-derived EVs. (A) Categorization of EV populations by size distribution by TRPS. The majority of EVs belong to a size range of 100-250 nm. t-test was performed between their counterparts. (B) pH affects the zeta potential of EVs: Compared to physiological pH (7.4), cyst microenvironmental pH (6.0) showed a drastic change in the zeta potential (measured by TRPS) of EVs derived from T1G cells. (C) Western blot analysis of EVs. Classical EV markers such as Alix, TSG 101, ARL13b, CD63, CD81, and CD9 were present in EVs derived from mIMCD3 and T1G cells. Data ( n \u003d 3 ) were represented as mean +- SD. Significant difference analyzed by t -test (* p \u003c 0.05, ** p \u003c 0.01, *** p \u003c 0.001).','shortDescription':'FIGURE 2 Particle population characteristics, zeta potential, and western blot analysis of cell-derived EVs. (A) Categorization of EV populations by size distribution by TRPS. The majority of EVs belong to a size range of 100-250 nm. t-test was performed between their counterparts. (B) pH affects the zeta potential of EVs: Compared to physiological pH (7.4), cyst microenvironmental pH (6.0) showed a drastic... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Jul 16, 2021, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Frontiers in physiology','journalText':'Frontiers in physiology 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_27424_fphys-12-630933-g002.jpg','benchSciPubmedId':'34262466'},{'sku':'10626D','imageId':'756116','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_9657_sensors-20-00965-g006.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_9657_sensors-20-00965-g006.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_9657_sensors-20-00965-g006.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_9657_sensors-20-00965-g006.jpg?time\u003d20240507','sortOrder':'3','description':'Figure 6 Confocal microscopy to evaluate the relative expression of CD9, CD63, CD81, CD24, CD44, CD54, CD326 and CD340 membrane protein markers in the exosomes derived from MCF7, MDA-MB-231 and SKBr3 breast cancer cell lines. Magnetic particles appear stained in green while the membrane protein receptors, in red, represents a positive expression on the membrane of the exosomes. In all cases, the primary antibody was 5 mug mL -1 and 2 µg mL -1 of antimouse-Cy5 antibody.','shortDescription':'Figure 6 Confocal microscopy to evaluate the relative expression of CD9, CD63, CD81, CD24, CD44, CD54, CD326 and CD340 membrane protein markers in the exosomes derived from MCF7, MDA-MB-231 and SKBr3 breast cancer cell lines. Magnetic particles appear stained in green while the membrane protein receptors, in red, represents a positive expression on the membrane of the exosomes. In all cases, the primary ant... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Immunocytochemistry (ICC/IF)','title':'CD9 Antibody (10626D) in ICC/IF','appAbv':'ICC/IF','dateTime':'Feb 11, 2020, 5:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Sensors (Basel, Switzerland)','journalText':'Sensors (Basel, Switzerland) 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_9657_sensors-20-00965-g006.jpg','benchSciPubmedId':'32054015'},{'sku':'10626D','imageId':'1111640','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_30062_41232_2023_299_Fig5_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_30062_41232_2023_299_Fig5_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_30062_41232_2023_299_Fig5_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_30062_41232_2023_299_Fig5_HTML.jpg?time\u003d20240507','sortOrder':'4','description':'Collection of small extracellular vesicles (sEVs) by ultracentrifugation (UC) and tangential flow filtration (TTF). A Particle size distribution of sEVs when collected by UC and TFF. B Particle number collected from the same volume (200 ml) of mesenchymal stem cell (MSC) culture supernatant. Western blot analysis of CD9 ( C ), and CD81 ( D ) of sEVs collected using UC and TFF. n \u003d 3 per experiment. *** p \u003c 0.001, Mann-Whitney U analysis','shortDescription':'Collection of small extracellular vesicles (sEVs) by ultracentrifugation (UC) and tangential flow filtration (TTF). A Particle size distribution of sEVs when collected by UC and TFF. B Particle number collected from the same volume (200 ml) of mesenchymal stem cell (MSC) culture supernatant. Western blot analysis of CD9 ( C ), and CD81 ( D ) of sEVs collected using UC and TFF. n \u003d 3 per experiment. *** p \u003c ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Oct 9, 2023, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Inflammation and regeneration','journalText':'Inflammation and regeneration 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_30062_41232_2023_299_Fig5_HTML.jpg','benchSciPubmedId':'37814342'},{'sku':'10626D','imageId':'1131209','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_29696_ijms-24-08506-g002.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_29696_ijms-24-08506-g002.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_29696_ijms-24-08506-g002.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_29696_ijms-24-08506-g002.jpg?time\u003d20240507','sortOrder':'5','description':'Molecular characterization of EVs from human A375 melanoma cells and human M12 melanoma brain metastases cells. ( a ) Schematic representation of samples. ( b ) Western blot images of intracellular vesicle contaminant marker calnexin and EV cytosolic (ALIX, TSG101, and annexin V) and membrane (CD63, CD9, and CD81) markers.','shortDescription':'Molecular characterization of EVs from human A375 melanoma cells and human M12 melanoma brain metastases cells. ( a ) Schematic representation of samples. ( b ) Western blot images of intracellular vesicle contaminant marker calnexin and EV cytosolic (ALIX, TSG101, and annexin V) and membrane (CD63, CD9, and CD81) markers.','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'May 9, 2023, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'International journal of molecular sciences','journalText':'International journal of molecular sciences 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_29696_ijms-24-08506-g002.jpg','benchSciPubmedId':'37239852'},{'sku':'10626D','imageId':'1131243','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_29674_10020_2023_659_Fig1_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_29674_10020_2023_659_Fig1_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_29674_10020_2023_659_Fig1_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_29674_10020_2023_659_Fig1_HTML.jpg?time\u003d20240507','sortOrder':'6','description':'Characterization of plasma sEVs. sEVs were freshly isolated from plasma of HNC, NED patients and HD by size-exclusion chromatography and characterized for their morphology, particle concentration and protein content. A Representative TEM images of sEVs from HNC, NED patients and HD are shown (magnification \u003d 25.000x, scale \u003d 100 nm). B Size distribution, concentration and median diameter of the particles were detected using NTA. C NTA particle concentration of HNC, NED patients and HD were visualized by scatter plots. D Comparison of total protein content in 1 mL of isolated sEVs measured by BCA protein assays. E Western blots were performed using 10 mug protein and the presence of typical vesicle proteins CD9, CD63 and TSG101 and the absence of contaminating proteins (Grp94, ApoA1) were shown. P values below 0.05 were considered as significant (*p \u003c 0.05)','shortDescription':'Characterization of plasma sEVs. sEVs were freshly isolated from plasma of HNC, NED patients and HD by size-exclusion chromatography and characterized for their morphology, particle concentration and protein content. A Representative TEM images of sEVs from HNC, NED patients and HD are shown (magnification \u003d 25.000x, scale \u003d 100 nm). B Size distribution, concentration and median diameter of the particles we... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'May 24, 2023, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Molecular medicine (Cambridge, Mass.)','journalText':'Molecular medicine (Cambridge, Mass.) 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_29674_10020_2023_659_Fig1_HTML.jpg','benchSciPubmedId':'37226100'},{'sku':'10626D','imageId':'1071825','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_27422_fphys-14-1143966-g002.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_27422_fphys-14-1143966-g002.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_27422_fphys-14-1143966-g002.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_27422_fphys-14-1143966-g002.jpg?time\u003d20240507','sortOrder':'7','description':'FIGURE 2 Characterization of myotube derived extracellular vesicles (EVs). Human myotubes were exposed to electrical pulse stimulation (EPS) for 24 h, and cell derived EVs were collected for 24 h thereafter. Size (A) and concentration (C) of exosomes (EXO) and microvesicles (MV) were measured by nanoparticle tracking analysis (NTA). The presence of EV markers on exosomes and MV captured by anti-CD81-coated magnetic beads were detected with PE-conjugated CD81 (B) and CD63 (D) antibodies by flow-cytometry (BD Accuri C6 flow cytometer). Presence of CD9, calnexin, and heat shock protein 70 (Hsc/Hsp70) on exosomes were measured by Western blotting (E) . Cell lysates of SW480 cells (SW) were used as control. Transmission electron microscopy (TEM) images of skeletal muscle cell derived EVs (F) . 1. Freshly isolated EVs from conditioned media from human myotubes, scale bar 1 um. 2. Close up of framed EVs in picture 1, scale bar 200 nm. Data are presented as mean +- SEM ( n \u003d 6 in each group). MFI \u003d mean fluorescence intensity.','shortDescription':'FIGURE 2 Characterization of myotube derived extracellular vesicles (EVs). Human myotubes were exposed to electrical pulse stimulation (EPS) for 24 h, and cell derived EVs were collected for 24 h thereafter. Size (A) and concentration (C) of exosomes (EXO) and microvesicles (MV) were measured by nanoparticle tracking analysis (NTA). The presence of EV markers on exosomes and MV captured by anti-CD81-coated ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Apr 18, 2023, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Frontiers in physiology','journalText':'Frontiers in physiology 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_27422_fphys-14-1143966-g002.jpg','benchSciPubmedId':'37064893'},{'sku':'10626D','imageId':'1067396','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_27421_pharmaceutics-15-00953-g001.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_27421_pharmaceutics-15-00953-g001.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_27421_pharmaceutics-15-00953-g001.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_27421_pharmaceutics-15-00953-g001.jpg?time\u003d20240507','sortOrder':'8','description':'Characterization of platelets and isolated platelet-derived extracellular vesicles (pEV). ( A ) Schematic overview of the density gradient ultracentrifugation (DGUC) protocol used to isolate pEV from expired platelet concentrates (PC). ( B ) Expression of platelet activation marker CD62p in basal and thrombin-stimulated platelets ( n \u003d 3; two-tailed unpaired t -test, *** p \u003c 0.001). ( C ) Representative transmission electron microscopy (TEM) micrographs of platelets from the expired PC (activated platelets are indicated by black arrows and resting platelets by red arrows). Scale bars: 1 mum. ( D ) Western blot analysis of specific EV markers (tetraspanins CD63 (glycosylated form) and CD9, and cytosolic protein flotillin-2), platelet-specific marker (CD41), and non-EV markers [apolipoprotein (ApoA1) and argonaute 2 (Ago2)] in pooled fractions and platelet lysate (PLTS). Molecular weight (MW) markers are indicated. ( E ) Representative TEM images of negatively stained 8-9 fractions, enriched in cup-shaped pEV (black arrows). Higher magnification image detailing the morphology of pEV. Scale bars: 1 um and 200 nm (high magnification). ( F ) Representative size distribution profiles of 8-9 pooled pEV-fractions analyzed using nanoparticle tracking analysis. Size distribution is represented as mean (black continuous line) +- standard deviation (shaded area).','shortDescription':'Characterization of platelets and isolated platelet-derived extracellular vesicles (pEV). ( A ) Schematic overview of the density gradient ultracentrifugation (DGUC) protocol used to isolate pEV from expired platelet concentrates (PC). ( B ) Expression of platelet activation marker CD62p in basal and thrombin-stimulated platelets ( n \u003d 3; two-tailed unpaired t -test, *** p \u003c 0.001). ( C ) Representative tra... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Mar 15, 2023, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Pharmaceutics','journalText':'Pharmaceutics 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_27421_pharmaceutics-15-00953-g001.jpg','benchSciPubmedId':'36986815'},{'sku':'10626D','imageId':'1067372','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_27420_fimmu-14-1107150-g001.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_27420_fimmu-14-1107150-g001.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_27420_fimmu-14-1107150-g001.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_27420_fimmu-14-1107150-g001.jpg?time\u003d20240507','sortOrder':'9','description':'Characterization of exosomes. (A) Concentration and size distribution of exosomes isolated from patients and healthy volunteers\u0027 plasma, nanoparticle tracking analysis (NTA); (B) CD63, CD81 and CD9 expression in plasma-isolated exosomes from healthy volunteers (Lines 1-4) and polytrauma patients (Lines 5-7), Representative Western-blot analysis.','shortDescription':'Characterization of exosomes. (A) Concentration and size distribution of exosomes isolated from patients and healthy volunteers\u0027 plasma, nanoparticle tracking analysis (NTA); (B) CD63, CD81 and CD9 expression in plasma-isolated exosomes from healthy volunteers (Lines 1-4) and polytrauma patients (Lines 5-7), Representative Western-blot analysis.','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Mar 28, 2023, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Frontiers in immunology','journalText':'Frontiers in immunology 2023 - ','sourceType':'3','benchsci':true,'imageName':'tfs_27420_fimmu-14-1107150-g001.jpg','benchSciPubmedId':'36969201'},{'sku':'10626D','imageId':'954612','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_21105_12964_2022_863_Fig1_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_21105_12964_2022_863_Fig1_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_21105_12964_2022_863_Fig1_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_21105_12964_2022_863_Fig1_HTML.jpg?time\u003d20240507','sortOrder':'10','description':'Characterization of human oligodendroglia-derived extracellular vesicles. A Western blotting analysis of small extracellular vesicle protein markers CD63, CD9, Flotillin-1, Annexin A2, HSP70, and the negative control marker GRP94. B Representative overview (scale bar 200 nm) and cropped (scale bar 100 nm) image showing the morphology, as revealed by transmission electron microscopy, of EVs isolated from wild-type native human oligodendroglia (OL-EVs) and stably expressing HSPB8 oligodendroglia (OL-HSPB8-EV). C - E Particle size profile distribution, particles per mL, and total EV protein measured by Nanoparticle Tracking Analysis (NTA) and BCA of OL-EVs and OL-HSPB8-EVs, pelleted from 30 mL of conditioned medium. F - H Western blotting and qPCR analysis of HSPB8 present in OL-EVs and OL-HSPB8-EVs. Statistical analysis was performed using GraphPad. Student\u0027s t-test was used to determine significance (** P \u003c 0.01, **** P \u003c 0.0001). Data are presented as mean +- SEM','shortDescription':'Characterization of human oligodendroglia-derived extracellular vesicles. A Western blotting analysis of small extracellular vesicle protein markers CD63, CD9, Flotillin-1, Annexin A2, HSP70, and the negative control marker GRP94. B Representative overview (scale bar 200 nm) and cropped (scale bar 100 nm) image showing the morphology, as revealed by transmission electron microscopy, of EVs isolated from wil... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'May 5, 2022, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Cell communication and signaling : CCS','journalText':'Cell communication and signaling : CCS 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_21105_12964_2022_863_Fig1_HTML.jpg','benchSciPubmedId':'35513867'},{'sku':'10626D','imageId':'949704','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_20099_41598_2021_1334_Fig2_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_20099_41598_2021_1334_Fig2_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_20099_41598_2021_1334_Fig2_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_20099_41598_2021_1334_Fig2_HTML.jpg?time\u003d20240507','sortOrder':'11','description':'Figure 2 Western blot detection of CD9, Hsc70/Hsp70 and Calnexin in plasma pool EVs. As a positive control, lysate from the colorectal cancer cell line SW480 was used. Full-length blots are presented in Supplementary Fig. 3 (Supplementary Information).','shortDescription':'Figure 2 Western blot detection of CD9, Hsc70/Hsp70 and Calnexin in plasma pool EVs. As a positive control, lysate from the colorectal cancer cell line SW480 was used. Full-length blots are presented in Supplementary Fig. 3 (Supplementary Information).','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Nov 9, 2021, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Scientific reports','journalText':'Scientific reports 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_20099_41598_2021_1334_Fig2_HTML.jpg','benchSciPubmedId':'34754007'},{'sku':'10626D','imageId':'949540','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_20030_41467_2021_26485_Fig5_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_20030_41467_2021_26485_Fig5_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_20030_41467_2021_26485_Fig5_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_20030_41467_2021_26485_Fig5_HTML.jpg?time\u003d20240507','sortOrder':'12','description':'Fig. 5 MAAP promotes association of AAV with EVs. A Schematic of EV isolation by iodixanol density gradient from HEK293 suspension culture. B Immunoblots of iodixanol fractions from suspension cells producing recombinant MAAP8Delta vector complemented in trans with CMV-HA and C CMV-MAAP8-HA. EVs, capsid and MAAP were analyzed from the media of HEK293 producing cells at day 3 post transfection. Capsid and MAAP proteins were analyzed by SDS-PAGE under reducing conditions while EV markers (CD81, CD63, CD9) were analyzed by SDS-PAGE under non-reducing conditions ( n \u003d 2). D Graph displaying percent vector genome titer relative to total viral genomes for each fraction from iodixanol gradient purified MAAP8Delta vector complemented in trans with CMV-HA and CMV-MAAP8-HA. E Schematic of EV isolation by size exclusion chromatography (SEC) from HEK293 suspension culture. F Immunoblots of SEC fractions from suspension cells producing recombinant MAAP8Delta vector complemented in trans with CMV-HA and G CMV-MAAP8-HA. EVs and MAAP were analyzed from the media of HEK293 producing cells at day 3 post transfection. MAAP protein was analyzed by SDS-PAGE under reducing conditions while the EV marker CD63 was analyzed by SDS-PAGE under non-reducing conditions ( n \u003d 2). H Graph displaying percent vector genome titer relative to total viral genomes for each fraction from SEC purified MAAP8Delta vector complemented in trans with CMV-HA and CMV-MAAP8-HA.','shortDescription':'Fig. 5 MAAP promotes association of AAV with EVs. A Schematic of EV isolation by iodixanol density gradient from HEK293 suspension culture. B Immunoblots of iodixanol fractions from suspension cells producing recombinant MAAP8Delta vector complemented in trans with CMV-HA and C CMV-MAAP8-HA. EVs, capsid and MAAP were analyzed from the media of HEK293 producing cells at day 3 post transfection. Capsid and MA... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Oct 29, 2021, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Nature communications','journalText':'Nature communications 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_20030_41467_2021_26485_Fig5_HTML.jpg','benchSciPubmedId':'34716331'},{'sku':'10626D','imageId':'877755','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_17750_fimmu-12-732209-g002.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_17750_fimmu-12-732209-g002.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_17750_fimmu-12-732209-g002.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_17750_fimmu-12-732209-g002.jpg?time\u003d20240507','sortOrder':'13','description':'Figure 2 HR promoted the concentration of EVs and CD203c + -EVs and CD63 + -EVs derived from serum. 500mul serum from control and different time point HR groups were used to pellet EVs, finally, the EVs were resuspended in 200mul PBS. The total concentration of EVs was determined by a BCA assay. The protein expression levels of CD63, CD203c, CD9 and CD81 in EVs/serum were identified using western blotting. (A) Total concentration of EVs isolated from same volume serum of control and different time point HR groups, n \u003d 10. (B-F) Relative CD63, CD203c, CD9 and CD81 protein expression levels of EVs derived from same volume of serum in control and HR groups, 30mul EVs/lane, n \u003d 5 (G-I) Relative CD63 and CD203c expression levels in same volume of serum in the control and HR groups, 30mul serum/lane, n \u003d 5. All values represent the mean +- standard deviation. Difference to control: ** p \u003c 0,01; *** p \u003c 0,001; # Difference between groups: # p \u003c 0,05; ## p \u003c 0,01; 1 way ANOVA with Newman-Keuls Multiple Comparison Test.','shortDescription':'Figure 2 HR promoted the concentration of EVs and CD203c + -EVs and CD63 + -EVs derived from serum. 500mul serum from control and different time point HR groups were used to pellet EVs, finally, the EVs were resuspended in 200mul PBS. The total concentration of EVs was determined by a BCA assay. The protein expression levels of CD63, CD203c, CD9 and CD81 in EVs/serum were identified using western blotting. ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Dec 24, 2021, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Frontiers in immunology','journalText':'Frontiers in immunology 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_17750_fimmu-12-732209-g002.jpg','benchSciPubmedId':'34650557'},{'sku':'10626D','imageId':'949301','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_17750_fimmu-12-732209-g001.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_17750_fimmu-12-732209-g001.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_17750_fimmu-12-732209-g001.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_17750_fimmu-12-732209-g001.jpg?time\u003d20240507','sortOrder':'14','description':'Figure 1 Characterization of control EVs and HR-EVs. EVs were processed for western blot, TEM and NTA after isolated from 500mul serum of control and HR groups. (A) The same amount (30mul) of control EVs, HR-EVs and EVs-depleted-Serum were identified using western blot. Representative western blot image showing bands of standard surface markers (CD9, CD63, CD81) of control EVs and HR-EVs. (B, C) Morphology of control EVs and HR-EVs was monitored by TEM; Scale bar: 100 nm. (D, E) Particle size distribution of control EVs and HR-EVs was determined by NTA. Distribution of EVs with a size of 90-200 nm in diameter in both groups. (F, G) Quantitative comparison between control EVs and HR-EVs in count and size measured by NTA; n \u003d 10; All values represent mean +- standard deviation. * p \u003c 0,05, independent two-tailed Student\u0027s t -tests.','shortDescription':'Figure 1 Characterization of control EVs and HR-EVs. EVs were processed for western blot, TEM and NTA after isolated from 500mul serum of control and HR groups. (A) The same amount (30mul) of control EVs, HR-EVs and EVs-depleted-Serum were identified using western blot. Representative western blot image showing bands of standard surface markers (CD9, CD63, CD81) of control EVs and HR-EVs. (B, C) Morphology ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Dec 24, 2021, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Frontiers in immunology','journalText':'Frontiers in immunology 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_17750_fimmu-12-732209-g001.jpg','benchSciPubmedId':'34650557'},{'sku':'10626D','imageId':'878484','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_15302_mmr-24-05-12455-g04.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_15302_mmr-24-05-12455-g04.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_15302_mmr-24-05-12455-g04.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_15302_mmr-24-05-12455-g04.jpg?time\u003d20240507','sortOrder':'15','description':'Figure 5. Release of exosomes from Mtb H37Rv-infected or naive THP-1 cells. THP-1 macrophages were infected with Mtb H37Rv (Mtb + ) for 4 h (MOI 5) in DCM-UG medium for 4, 24 and 48 h at 37degC and in the presence of 5% CO 2 . Exosomes were extracted from cell culture supernatants using total exosome isolation reagent and were further analyzed by western blot analysis for the exosomal protein markers, (A) CD63, CD81, CD9 and (B) LAMP-1, which served as a positive control for exosomes signal and Mtb proteins [Ag85 and Mpt64 and recombinant Mtb Mpt64 protein (His-tag)]. Data from one representative experiment out of at least three independent experiments are shown. MOI, multiplicity of infection; Mtb, Mycobacterium tuberculosis ; DCM-UG, ultra-centrifuged CellGenix (r) GMP DC Medium; LAMP-1, lysosomal associated membrane protein-1; Ag85, mycobacterial antigen 85.','shortDescription':'Figure 5. Release of exosomes from Mtb H37Rv-infected or naive THP-1 cells. THP-1 macrophages were infected with Mtb H37Rv (Mtb + ) for 4 h (MOI 5) in DCM-UG medium for 4, 24 and 48 h at 37degC and in the presence of 5% CO 2 . Exosomes were extracted from cell culture supernatants using total exosome isolation reagent and were further analyzed by western blot analysis for the exosomal protein markers, (A) C... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Nov 1, 2021, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Molecular medicine reports','journalText':'Molecular medicine reports 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_15302_mmr-24-05-12455-g04.jpg','benchSciPubmedId':'34558650'},{'sku':'10626D','imageId':'877047','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_17054_cancers-13-04176-g001.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_17054_cancers-13-04176-g001.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_17054_cancers-13-04176-g001.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_17054_cancers-13-04176-g001.jpg?time\u003d20240507','sortOrder':'16','description':'Figure 1 Characterization of small extracellular vesicles (sEVs) derived from human Head and Neck Squamous Cell Carcinoma cell lines: FaDu (hypopharyngeal squamous cell carcinoma), PCI-30 (tongue squamous cell carcinoma), SCC-25 (tongue squamous cell carcinoma); and sEVs derived from HaCat (immortalized human keratinocytes). ( A ) Representative cryogenic transmission microscopy image of HNSCC cell-derived sEVs. ( B ) Representative concentration and size distribution plot of HNSCC-derived sEVs measured by nanoparticle tracking analysis (NTA) and particle visualization based on Brownian motions. ( C ) Immunoblotting of sEV markers CD63, CD9, and negative marker Grp94 in HNSCC-derived sEVs. Full blot images are presented in Figures S2-S4 . ( D ) Particle concentration related to normoxic (21% O 2 ) and hypoxic (1% O 2 , 5% O 2 , 10% O 2 ) conditions. Results were obtained using NTA and normalized to the protein levels in lysates of producer cells. ( E ) Particle diameter related to normoxic (21% O 2 ) and hypoxic (1% O 2 , 5% O 2 , 10% O 2 ) conditions. Results were obtained using NTA. All data represent three biological replicates and are presented as means +- SD. * p \u003c 0.05 vs. 21%; ** p \u003c 0.01 vs. 21%; *** p \u003c 0.001 vs. 21%.','shortDescription':'Figure 1 Characterization of small extracellular vesicles (sEVs) derived from human Head and Neck Squamous Cell Carcinoma cell lines: FaDu (hypopharyngeal squamous cell carcinoma), PCI-30 (tongue squamous cell carcinoma), SCC-25 (tongue squamous cell carcinoma); and sEVs derived from HaCat (immortalized human keratinocytes). ( A ) Representative cryogenic transmission microscopy image of HNSCC cell-derived ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Aug 19, 2021, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Cancers','journalText':'Cancers 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_17054_cancers-13-04176-g001.jpg','benchSciPubmedId':'34439329'},{'sku':'10626D','imageId':'876070','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_17590_41419_2021_4069_Fig1_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_17590_41419_2021_4069_Fig1_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_17590_41419_2021_4069_Fig1_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_17590_41419_2021_4069_Fig1_HTML.jpg?time\u003d20240507','sortOrder':'17','description':'Fig. 1 Characterization of tumor microvesicles. A Particle volume distribution in function of sizes of plasma tumor MVs (T-MVs) from giant cell tumor of bone patient 1, healthy control microvesicles (C-MVs), T-MVs from patient 2, and patient 3, as indicated. B Distribution of particle concentrations in function of sizes in a representative preparation of plasma T-MVs determined by nanoparticle-tracking analysis (NanoSight). Histogram of typical preparations containing 10 9 T-MVs/ml. C Flow cytometry scatter plot of T-MVs showing autofluorescence settings and staining with 7-AAD, indicating high integrity of isolated MVs. D Representative fluorescence activated cell sorting (FACS) analysis of healthy subjects\u0027 control MVs (C-MVs) and T-MVs detected by CD63 and CD9 antibodies and cytometric analysis of CD44, and CD117 antigen expression in T-MVs of different patients. Percent of positive MVs are indicated in each quadrant. E Western blots of 30 ug protein extracts from isolated MVs. Lanes C1-C3; proteins from healthy control microvesicles (C-MVs). Lanes T1-T7, proteins from tumor patient microvesicles (T-MVs). Specific antibodies used for immunoblots are indicated. F Upper panel: red PONCEAU blots of 30 ug protein extracts from isolated MVs. Lane M; marker, Lanes C1-C3; proteins from healthy control microvesicles (C-MVs). Lanes T1-T7, proteins from tumor patient microvesicles (T-MVs). Lower panel western blot for Cytochrome C determination (negative control). Tubulin was used a','shortDescription':'Fig. 1 Characterization of tumor microvesicles. A Particle volume distribution in function of sizes of plasma tumor MVs (T-MVs) from giant cell tumor of bone patient 1, healthy control microvesicles (C-MVs), T-MVs from patient 2, and patient 3, as indicated. B Distribution of particle concentrations in function of sizes in a representative preparation of plasma T-MVs determined by nanoparticle-tracking anal... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Aug 17, 2021, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Cell death \u0026 disease','journalText':'Cell death \u0026 disease 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_17590_41419_2021_4069_Fig1_HTML.jpg','benchSciPubmedId':'34404763'},{'sku':'10626D','imageId':'879188','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_16901_fmolb-08-685088-g002.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_16901_fmolb-08-685088-g002.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_16901_fmolb-08-685088-g002.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_16901_fmolb-08-685088-g002.jpg?time\u003d20240507','sortOrder':'18','description':'FIGURE 2 Characterization of control EVs and gene analysis of hsa-124-3p in serum-derived EVs and serum. (A) Representative image of Western blotting showed bands of standard surface markers ( CD9 , CD63 , and CD81 ) of EVs and EV-depleted serum. (B) Morphology of EVs was monitored by TEM; scale bar: 100 nm. (C,D) Particle size distribution of EVs was determined by NTA. (E,F) Gene expression of hsa-miR-124-3p in EVs and serum of different samples at different time points. Compared to the control group: * p \u003c 0.05; ** p \u003c 0.01; *** p \u003c 0.001; **** p \u003c 0.0001, # Difference between groups: # p \u003c 0.05; ## p \u003c 0.01; #### p \u003c 0.0001; one-way ANOVA with the Newman-Keuls multiple comparison test.','shortDescription':'FIGURE 2 Characterization of control EVs and gene analysis of hsa-124-3p in serum-derived EVs and serum. (A) Representative image of Western blotting showed bands of standard surface markers ( CD9 , CD63 , and CD81 ) of EVs and EV-depleted serum. (B) Morphology of EVs was monitored by TEM; scale bar: 100 nm. (C,D) Particle size distribution of EVs was determined by NTA. (E,F) Gene expression of hsa-miR-124-... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Jul 20, 2021, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Frontiers in molecular biosciences','journalText':'Frontiers in molecular biosciences 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_16901_fmolb-08-685088-g002.jpg','benchSciPubmedId':'34277703'},{'sku':'10626D','imageId':'847115','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_14108_13287_2021_2317_Fig3_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_14108_13287_2021_2317_Fig3_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_14108_13287_2021_2317_Fig3_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_14108_13287_2021_2317_Fig3_HTML.jpg?time\u003d20240507','sortOrder':'19','description':'Fig. 3 Characterization of control EVs and Cur-EVs. a Representative western blot image showing bands of standard surface markers (CD9, CD63, CD81) of control EV- and Cur-EV lysates. b , c Morphology of control EVs and Cur-EVs was monitored by TEM; scale bar, 100 nm. d , e Particle size distribution of control EVs and Cur-EVs was measured by NTA. f , g Quantitative comparison between control EVs and Cur-EVs in count and size measured by NTA; n \u003d 3. h Absorbance at 420 nm of control EVs and Cur-EVs was determined with by spectrophotometry indicating presence of curcumin in Cur-EVs; n \u003d 3. i Cell nuclei were stained with DAPI (blue) and chondrocytes were stained with Phalloidin (green) to visualize the structure of the cytoskeleton. PKH26-labeled control EVs (red) and Cur-EVs (red) were internalized by chondrocytes and visualized with fluorescent microscopy','shortDescription':'Fig. 3 Characterization of control EVs and Cur-EVs. a Representative western blot image showing bands of standard surface markers (CD9, CD63, CD81) of control EV- and Cur-EV lysates. b , c Morphology of control EVs and Cur-EVs was monitored by TEM; scale bar, 100 nm. d , e Particle size distribution of control EVs and Cur-EVs was measured by NTA. f , g Quantitative comparison between control EVs and Cur-EVs... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Apr 29, 2021, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Stem cell research \u0026 therapy','journalText':'Stem cell research \u0026 therapy 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_14108_13287_2021_2317_Fig3_HTML.jpg','benchSciPubmedId':'33926561'},{'sku':'10626D','imageId':'847059','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_14078_pharmaceuticals-14-00356-g001.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_14078_pharmaceuticals-14-00356-g001.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_14078_pharmaceuticals-14-00356-g001.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_14078_pharmaceuticals-14-00356-g001.jpg?time\u003d20240507','sortOrder':'20','description':'Figure 1 Characterization of extracellular vesicles (EVs) loaded with CRISPR associated protein 9 (Cas9). ( a ) Schematic of loading procedure. The conditioned medium of MDA-MB-231 human breast cancer cells was collected (step 1) and tangential flow filtration (TFF) was performed to remove components larger than 650 nm and smaller than 500 kDa (step 2). The isolated EVs were gently mixed with PULSin/Cas9 (step 3) and size-exclusion chromatography (SEC) was used to remove free Cas9, PULSin and Cas9/PULSin (step 4). ( b ) Mean size determined by nanoparticle tracking analysis (NTA). ( c ) Zeta potential measured by laser Doppler micro-electrophoresis. ( d ) Cas9 loading and protein markers of EVs, heat-shock cognate protein 71 (Hsc70), cluster of differentiation (CD)63, CD9 and annexin V, and intracellular contaminant marker, calnexin, detected by Western blot. Data are presented as mean +- SD of three biological replicates (b and c). Statistical analysis was performed by one-way analysis of variance (ANOVA) with post-hoc pairwise comparisons using Tukey\u0027\u0027s test. *, p \u003c 0.05; ****, p \u003c 0.0001.','shortDescription':'Figure 1 Characterization of extracellular vesicles (EVs) loaded with CRISPR associated protein 9 (Cas9). ( a ) Schematic of loading procedure. The conditioned medium of MDA-MB-231 human breast cancer cells was collected (step 1) and tangential flow filtration (TFF) was performed to remove components larger than 650 nm and smaller than 500 kDa (step 2). The isolated EVs were gently mixed with PULSin/Cas9 (s... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Apr 13, 2021, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Pharmaceuticals (Basel, Switzerland)','journalText':'Pharmaceuticals (Basel, Switzerland) 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_14078_pharmaceuticals-14-00356-g001.jpg','benchSciPubmedId':'33924377'},{'sku':'10626D','imageId':'948848','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_19715_fbioe-08-615520-g005.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_19715_fbioe-08-615520-g005.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_19715_fbioe-08-615520-g005.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_19715_fbioe-08-615520-g005.jpg?time\u003d20240507','sortOrder':'21','description':'FIGURE 5 EV characterization and validation. (A) Representative SEM pictures of BMSC EVs. White arrows label EVs with 97 nm (left image) and 115 nm (right image) diameter. Magnification: 60,000x. (B) Representative TEM pictures of BMSC EVs. Scale bar left image \u003d 200 nm, magnification: 100,000x. Right image shows magnification of the EV in the green box. The size of objects was determined (red lines). Vertical line \u003d 104 nm, horizontal line \u003d 113 nm. (C) Western Blot analysis of EV specific surface markers CD9 (left image) and CD81 (right image) in BMSC, CA, CA/OP, and OP EVs and in the EV-depleted FCS depl - uc . Lane 1 \u003d MW ladder, Lane 2 \u003d 5 mug; Lanes 3-6 \u003d 8.2 mug; Lane 7 \u003d 10 mul; Exposure time: left image \u003d 3 min, right image \u003d 10 min (Pierce femto Kit). For respective Ponceau Red images, see Supplementary Figure 3 . (D) Test for EV uptake of BMSC-derived EVs into cultured BMSCs using PKH-26 (red) stained EVs (lower panel). PBS solution + PKH-26 stain was used as negative control (upper panel). Nuclei were counterstained with DAPI. Scale bar 100 mum.','shortDescription':'FIGURE 5 EV characterization and validation. (A) Representative SEM pictures of BMSC EVs. White arrows label EVs with 97 nm (left image) and 115 nm (right image) diameter. Magnification: 60,000x. (B) Representative TEM pictures of BMSC EVs. Scale bar left image \u003d 200 nm, magnification: 100,000x. Right image shows magnification of the EV in the green box. The size of objects was determined (red lines). Verti... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Jan 12, 2021, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Frontiers in bioengineering and biotechnology','journalText':'Frontiers in bioengineering and biotechnology 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_19715_fbioe-08-615520-g005.jpg','benchSciPubmedId':'33425878'},{'sku':'10626D','imageId':'948850','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_19715_Image_3.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_19715_Image_3.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_19715_Image_3.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_19715_Image_3.jpg?time\u003d20240507','sortOrder':'22','description':'Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot','shortDescription':'Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Jan 12, 2021, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Frontiers in bioengineering and biotechnology','journalText':'Frontiers in bioengineering and biotechnology 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_19715_Image_3.jpg','benchSciPubmedId':'33425878'},{'sku':'10626D','imageId':'948859','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_19719_fbioe-08-603598-g0003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_19719_fbioe-08-603598-g0003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_19719_fbioe-08-603598-g0003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_19719_fbioe-08-603598-g0003.jpg?time\u003d20240507','sortOrder':'23','description':'Figure 3 Characterization of EV-depleted FCS (FCS depl-uc ). Prior to ultracentrifugation, normal FCS was diluted in culture medium to different concentrations (20, 50%, and undiluted \u003d 100%); different FCS depl-uc groups were identified after ultracentrifugation. (A,B) FCS depl-uc-20% was controlled for EV surface makers (CD9, CD63, and CD81) detected by western blotting (lane 1: hBMSC lysate; lane 2: hBMSC-EVs; lane 3: undepleted FCS; lane 4: FCS depl-uc-20% ). Representative western blot image (A) and Ponceau Red-stained images for each surface marker (B) are shown; n \u003d 3. (C,D) Proliferation and apoptosis of hBMSC were determined by BrdU assay and caspase-3/7 activity assay separately after being incubated in culture medium supplemented with the different FCS groups for 24 h. All values represent mean +- standard deviation. *Significant difference to control: * p \u003c 0.05; ** p \u003c 0.01; # Significant difference between groups: # p \u003c 0.05; ### p \u003c 0.001 one-way ANOVA with Newman-Keuls Multiple Comparison Test; n \u003d 4.','shortDescription':'Figure 3 Characterization of EV-depleted FCS (FCS depl-uc ). Prior to ultracentrifugation, normal FCS was diluted in culture medium to different concentrations (20, 50%, and undiluted \u003d 100%); different FCS depl-uc groups were identified after ultracentrifugation. (A,B) FCS depl-uc-20% was controlled for EV surface makers (CD9, CD63, and CD81) detected by western blotting (lane 1: hBMSC lysate; lane 2: hBMS... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Jan 12, 2021, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Frontiers in bioengineering and biotechnology','journalText':'Frontiers in bioengineering and biotechnology 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_19719_fbioe-08-603598-g0003.jpg','benchSciPubmedId':'33425869'},{'sku':'10626D','imageId':'948856','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_19719_fbioe-08-603598-g0002.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_19719_fbioe-08-603598-g0002.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_19719_fbioe-08-603598-g0002.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_19719_fbioe-08-603598-g0002.jpg?time\u003d20240507','sortOrder':'24','description':'Figure 2 Characterization of hBMSC-derived EVs. (A) Representative western blot image ( n \u003d 4) demonstrates standard surface markers (CD9, CD63, and CD81) of hBMSC-derived EVs (lane 1: hBMSC lysate; lane 2: hBMSC-EVs). (B) Particle size distribution of hBMSC-EVs was measured by NTA. (C) Morphology of hBMSC-EVs was monitored by SEM, scale bar: 1 mum. (D) Cell nuclei were stained with DAPI (blue) and chondrocytes were stained with phalloidin (green) to visualize the cytoskeleton. PKH26-labeled hBMSC-derived EVs (red) internalized by chondrocytes were visualized with fluorescent microscopy.','shortDescription':'Figure 2 Characterization of hBMSC-derived EVs. (A) Representative western blot image ( n \u003d 4) demonstrates standard surface markers (CD9, CD63, and CD81) of hBMSC-derived EVs (lane 1: hBMSC lysate; lane 2: hBMSC-EVs). (B) Particle size distribution of hBMSC-EVs was measured by NTA. (C) Morphology of hBMSC-EVs was monitored by SEM, scale bar: 1 mum. (D) Cell nuclei were stained with DAPI (blue) and chondroc... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Jan 12, 2021, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Frontiers in bioengineering and biotechnology','journalText':'Frontiers in bioengineering and biotechnology 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_19719_fbioe-08-603598-g0002.jpg','benchSciPubmedId':'33425869'},{'sku':'10626D','imageId':'948749','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_19657_IJMM-46-06-2115-g00.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_19657_IJMM-46-06-2115-g00.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_19657_IJMM-46-06-2115-g00.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_19657_IJMM-46-06-2115-g00.jpg?time\u003d20240507','sortOrder':'25','description':'Figure 1 Characterization of OCEXs. (A) The sizes of the WSU-HN4- and SCC-9-derived exosomes were determined by NTA. (B) Representative images of exosomes derived from WSU-HN4 and SCC-9 cells, as detected by TEM. Scale bars, 50 nm. (C) Expression of the exosomal markers, Alix, CD63, CD9 and Rab5, in WSU-HN4- and SCC-9-derived exosomes was determined by western blot analysis. (D) Co-expressed proteins in WSU-HN4- and SCC-9-derived exosomes were detected by mass spectrometry. (E) Overlap of OCEX proteins and the top 100 frequently identified exosomal protein markers from the ExoCarta database. OCEXs, oral cancer-derived exosomes; NTA, nanoparticle tracking analysis; TEM, transmission electron microscopy; EXO/Exo, exosomes.','shortDescription':'Figure 1 Characterization of OCEXs. (A) The sizes of the WSU-HN4- and SCC-9-derived exosomes were determined by NTA. (B) Representative images of exosomes derived from WSU-HN4 and SCC-9 cells, as detected by TEM. Scale bars, 50 nm. (C) Expression of the exosomal markers, Alix, CD63, CD9 and Rab5, in WSU-HN4- and SCC-9-derived exosomes was determined by western blot analysis. (D) Co-expressed proteins in WSU... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Dec 1, 2020, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'International journal of molecular medicine','journalText':'International journal of molecular medicine 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_19657_IJMM-46-06-2115-g00.jpg','benchSciPubmedId':'33125101'},{'sku':'10626D','imageId':'837740','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_11761_pone.0238591.g001.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_11761_pone.0238591.g001.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_11761_pone.0238591.g001.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_11761_pone.0238591.g001.jpg?time\u003d20240507','sortOrder':'26','description':'Fig 1 Characterization of the small EVs isolated from the cell culture supernatant of the E10, BxPC3, and H3 cell lines. EVs were studied by protein concentration measurements (A), NTA for particle quantification (B), immunoaffinity capture targeting CD9 (C), and Western blot for the detection of CD9 in three sequential SEC fractions (20 mul per well) (D). Samples were negatively stained with 4% uranyl acetate (aqueous) for analysis by TEM (E). Data republished from Guerreiro et al [].','shortDescription':'Fig 1 Characterization of the small EVs isolated from the cell culture supernatant of the E10, BxPC3, and H3 cell lines. EVs were studied by protein concentration measurements (A), NTA for particle quantification (B), immunoaffinity capture targeting CD9 (C), and Western blot for the detection of CD9 in three sequential SEC fractions (20 mul per well) (D). Samples were negatively stained with 4% uranyl acet... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Oct 29, 2020, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'PloS one','journalText':'PloS one 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_11761_pone.0238591.g001.jpg','benchSciPubmedId':'32886718'},{'sku':'10626D','imageId':'756276','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_9770_cells-09-01946-g004.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_9770_cells-09-01946-g004.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_9770_cells-09-01946-g004.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_9770_cells-09-01946-g004.jpg?time\u003d20240507','sortOrder':'27','description':'Figure 4 Protein markers of plasma-derived EVs. ( a ) Protein concentration for each sample normalized to 1 mL of original plasma. ( b ) Western blot of cluster of differentiation 9 (CD9) (EV marker) and calnexin (contaminant marker). P is plasma. ( c ) Enzyme linked immunosorbent assay (ELISA) for apolipoprotein B (apoB). Data represent mean +- s.d. ( n \u003d 3) Statistics by one-way analysis of variance (ANOVA). * p \u003c 0.025; ** p \u003c 0.004; *** p \u003c 0.0007; ****, p \u003c 0.0001; ns, not significant.','shortDescription':'Figure 4 Protein markers of plasma-derived EVs. ( a ) Protein concentration for each sample normalized to 1 mL of original plasma. ( b ) Western blot of cluster of differentiation 9 (CD9) (EV marker) and calnexin (contaminant marker). P is plasma. ( c ) Enzyme linked immunosorbent assay (ELISA) for apolipoprotein B (apoB). Data represent mean +- s.d. ( n \u003d 3) Statistics by one-way analysis of variance (ANOV... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Aug 22, 2020, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Cells','journalText':'Cells 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_9770_cells-09-01946-g004.jpg','benchSciPubmedId':'32842648'},{'sku':'10626D','imageId':'837555','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_11647_fgene-11-00712-g004.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_11647_fgene-11-00712-g004.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_11647_fgene-11-00712-g004.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_11647_fgene-11-00712-g004.jpg?time\u003d20240507','sortOrder':'28','description':'FIGURE 4 Positive detection for specific exosomal surface markers (CD 63, CD9, and CD81) and for the control-positive marker (b-actin) by immunoblotting. (A) exosomes isolated by UC and (B) TEIp kit.','shortDescription':'FIGURE 4 Positive detection for specific exosomal surface markers (CD 63, CD9, and CD81) and for the control-positive marker (b-actin) by immunoblotting. (A) exosomes isolated by UC and (B) TEIp kit.','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Apr 16, 2022, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Frontiers in genetics','journalText':'Frontiers in genetics 2022 - ','sourceType':'3','benchsci':true,'imageName':'tfs_11647_fgene-11-00712-g004.jpg','benchSciPubmedId':'32793278'},{'sku':'10626D','imageId':'837719','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_11746_cancers-12-02110-g002.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_11746_cancers-12-02110-g002.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_11746_cancers-12-02110-g002.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_11746_cancers-12-02110-g002.jpg?time\u003d20240507','sortOrder':'29','description':'Figure 2 Successful isolation of exosomes from blood plasma was verified by Transmission Electron Microscopy (TEM), Western Blot, and Nanoparticle tracking. ( A ) Two representative TEM graphs showing negatively stained exosomes isolated from an HNSCC patient. As indicated by the size bars, exosomes vary in diameter between 30 and 150 nm and have round to oval shapes. Size bar on the top TEM graph \u003d 500 nm, size bar on the bottom TEM graph \u003d 200 nm. ( B ) Western Blot analysis of exosomes was performed to confirm the expression of exosomal markers TSG101, CD9 and CD63 and the expression of epithelial cell marker EpCAM (upper frame). Exosomes were also analyzed for negative markers ApoA1 and Grp94 along with plasma (diluted 50x in PBS) and cell lysate samples as positive controls. MW marker, positive control molecular weight marker. ( C ) Size distribution of exosomes was measured by nanoparticle tracking. The mean diameter was 86.8 nm. The maximal and minimal diameters were 257.5 nm and 22.5 nm, respectively. The 90th and 10th percentile were at 121.2 and 53.6 nm, respectively. ( D ) Protein content of exosomes was determined by Bicinchoninic Acid (BCA) Assay. Average protein content: 80.9 g/mL (HNSCC exosomes), 69.2 g/mL (healthy volunteer exosomes). n \u003d 23 (HNSCC), n \u003d 10 (NC). HNSCC, exosomes from blood plasma of HNSCC patients. NC \u003d no cancer, exosomes from blood plasma of healthy volunteers. ( E ) B cells that were not co-cultured with exosomes exhibited colony formation','shortDescription':'Figure 2 Successful isolation of exosomes from blood plasma was verified by Transmission Electron Microscopy (TEM), Western Blot, and Nanoparticle tracking. ( A ) Two representative TEM graphs showing negatively stained exosomes isolated from an HNSCC patient. As indicated by the size bars, exosomes vary in diameter between 30 and 150 nm and have round to oval shapes. Size bar on the top TEM graph \u003d 500 nm,... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Jul 29, 2020, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Cancers','journalText':'Cancers 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_11746_cancers-12-02110-g002.jpg','benchSciPubmedId':'32751214'},{'sku':'10626D','imageId':'846383','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_13727_ijms-21-03739-g001.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_13727_ijms-21-03739-g001.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_13727_ijms-21-03739-g001.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_13727_ijms-21-03739-g001.jpg?time\u003d20240507','sortOrder':'30','description':'Figure 1 Characterization of exosomes isolated from plasma. ( A ) Representative transmission electron microscopy (TEM) image of exosomes. Scalebar \u003d 200 nm. ( B ) Representative size distribution of exosomes measured by nanoparticle tracking analysis (NTA). ( C ) Exosomes derived from plasma of healthy donors (HD) and head and neck squamous cell carcinoma (HNSCC) patients were analyzed by Western blot for the presence of exosome specific markers using antibodies against CD63 and CD81 under non-reducing conditions and antibodies against CD9 and TSG101 under reducing conditions. Western blot analysis for the negative marker Grp94 and the apolipoprotein Apo1A was also performed for exosomes, cells, and plasma.','shortDescription':'Figure 1 Characterization of exosomes isolated from plasma. ( A ) Representative transmission electron microscopy (TEM) image of exosomes. Scalebar \u003d 200 nm. ( B ) Representative size distribution of exosomes measured by nanoparticle tracking analysis (NTA). ( C ) Exosomes derived from plasma of healthy donors (HD) and head and neck squamous cell carcinoma (HNSCC) patients were analyzed by Western blot for ... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'May 26, 2020, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'International journal of molecular sciences','journalText':'International journal of molecular sciences 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_13727_ijms-21-03739-g001.jpg','benchSciPubmedId':'32466374'},{'sku':'10626D','imageId':'838580','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_12307_18_2020_3507_Fig3_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_12307_18_2020_3507_Fig3_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_12307_18_2020_3507_Fig3_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_12307_18_2020_3507_Fig3_HTML.jpg?time\u003d20240507','sortOrder':'31','description':'Fig. 3 Effect of metalloproteinase inhibitors on ADAM10 internalization, degradation, and release in microvesicles. a , b THP-1 cells were treated with 10 muM GI or vehicle control. After 4 h ( a ) or 2 h ( b ), cells were analyzed for surface expression of ADAM10 by flow cytometry. The geometric mean fluorescence of treated cells was calculated in relation to that of the respective control and summarized as mean and SD of three independent experiments.) THP-1 cells were treated with 10 muM GI or vehicle control for 16 h in the absence or presence of 40 mM NH4Cl ( c ) or 0.5 muM bafilomycin A1 ( d ). Cell lysates were probed by western blotting with antibodies against the N-terminus of ADAM10 or against GAPDH as loading control. Data are shown as representative western blot and as relative changes of band intensity determined by densitometric analysis of three independent experiments. e-f THP-1 cells were treated with 10 muM GI, 10 muM TAPI or vehicle control for 24 h. Extracellular vesicles (EV) were prepared from conditioned cell media by differential centrifugation. Lysates and EV preparation were then subjected to western blot analysis with antibodies against the N-terminus of ADAM10, against the exosomal marker CD9 or against GAPDH as internal control ( e ). EV preparations from conditioned media were conjugated to beads which were then studied for ADAM10 immunoreactivity by flow cytometry ( f ). Data are shown as representative result or as means and SD of four independ','shortDescription':'Fig. 3 Effect of metalloproteinase inhibitors on ADAM10 internalization, degradation, and release in microvesicles. a , b THP-1 cells were treated with 10 muM GI or vehicle control. After 4 h ( a ) or 2 h ( b ), cells were analyzed for surface expression of ADAM10 by flow cytometry. The geometric mean fluorescence of treated cells was calculated in relation to that of the respective control and summarized a... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Jan 1, 2021, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Cellular and molecular life sciences : CMLS','journalText':'Cellular and molecular life sciences : CMLS 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_12307_18_2020_3507_Fig3_HTML.jpg','benchSciPubmedId':'32372373'},{'sku':'10626D','imageId':'837087','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_11322_41598_2020_62920_Fig1_HTML.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_11322_41598_2020_62920_Fig1_HTML.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_11322_41598_2020_62920_Fig1_HTML.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_11322_41598_2020_62920_Fig1_HTML.jpg?time\u003d20240507','sortOrder':'32','description':'Figure 1 ( a ) Nanoparticle Tracking analysis (NTA) of EVs derived from CRL-5908 cells isolated with three different methods: Yellow line indicates EVs isolated with one-step ultracentrifuge method, red line EVs isolated with commercial kit, and blue line EVs isolated with double-step ultracentrifuge method (peak indicated by arrow). EVs have a mean diameter of 133.7 +/- 6.5 nm and mode of 107.5 +/- 1.7 nm. EV-concentration is expressed as numbers of particles per mL, y axis is marked from 1 to 3,5 E10. ( b ) Western-blot image of CRL-5908 and CCL-185 cells lysates and their respective isolated EVs: EVs lysates showed higher expression of CD9, lower but presence of HSP70, and absence of GM130 in comparison with cell lines lysates.','shortDescription':'Figure 1 ( a ) Nanoparticle Tracking analysis (NTA) of EVs derived from CRL-5908 cells isolated with three different methods: Yellow line indicates EVs isolated with one-step ultracentrifuge method, red line EVs isolated with commercial kit, and blue line EVs isolated with double-step ultracentrifuge method (peak indicated by arrow). EVs have a mean diameter of 133.7 +/- 6.5 nm and mode of 107.5 +/- 1.7 nm.... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Apr 16, 2020, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Scientific reports','journalText':'Scientific reports 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_11322_41598_2020_62920_Fig1_HTML.jpg','benchSciPubmedId':'32300120'},{'sku':'10626D','imageId':'735251','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_7931_biomolecules-10-00150-g002.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_7931_biomolecules-10-00150-g002.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_7931_biomolecules-10-00150-g002.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_7931_biomolecules-10-00150-g002.jpg?time\u003d20240507','sortOrder':'33','description':'Figure 2 Extracellular vescile (EV) characterization. ( A ) Nanoparticle tracking analysis (NTA) showing a peak between 100-200 nm for the control (CT), luminal A (LA), and triple negative (TNBC) groups. ( B ) Transmission electron microscopy (TEM) image of EVs from cancer patient showing a size corresponding to NTA results. Size bar \u003d 200 nm. ( C ) Western blotting (WB) analysis showing strong protein expression of CD9 and CD63 on EVs from control (2) and cancer (4), when compared with corresponding serum supernatant EV-depleted from control (1) and cancer (3).','shortDescription':'Figure 2 Extracellular vescile (EV) characterization. ( A ) Nanoparticle tracking analysis (NTA) showing a peak between 100-200 nm for the control (CT), luminal A (LA), and triple negative (TNBC) groups. ( B ) Transmission electron microscopy (TEM) image of EVs from cancer patient showing a size corresponding to NTA results. Size bar \u003d 200 nm. ( C ) Western blotting (WB) analysis showing strong protein expr... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Jan 16, 2020, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Biomolecules','journalText':'Biomolecules 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_7931_biomolecules-10-00150-g002.jpg','benchSciPubmedId':'31963351'},{'sku':'10626D','imageId':'731205','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_6533_pone.0221679.g001.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_6533_pone.0221679.g001.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_6533_pone.0221679.g001.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_6533_pone.0221679.g001.jpg?time\u003d20240507','sortOrder':'34','description':'Fig 1 Morphological and biochemical analysis of isolated CAP exosomes. ( A ) Transmission electron microscopy pictures of parental (left) and modified (right) CAP exsomes. Scale bar represents 100 nm. ( B ) Western blot of 30 mug cellular and exosomal protein lysates per lane. Blotting performed on GRP78 (upper panel), turbo-GFP (middle panel) and CD9 protein (lower panel). Lane 1-4 represent cellular lysates, while lanes 5-8 depict exosomal lysate. ( C ) Quantification of Western blot Fig 1 B for CD9 signal. ( D ) Quantification of Western blot Fig 1 B for tGFP signal. ( E ) Quantification of Western blot Fig 1 B for GRP78 signal. ( F ) Flow cytometry analysis of GFP-positive exosomes isolated from parental and modified CAP cells.','shortDescription':'Fig 1 Morphological and biochemical analysis of isolated CAP exosomes. ( A ) Transmission electron microscopy pictures of parental (left) and modified (right) CAP exsomes. Scale bar represents 100 nm. ( B ) Western blot of 30 mug cellular and exosomal protein lysates per lane. Blotting performed on GRP78 (upper panel), turbo-GFP (middle panel) and CD9 protein (lower panel). Lane 1-4 represent cellular lysat... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Mar 3, 2020, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'PloS one','journalText':'PloS one 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_6533_pone.0221679.g001.jpg','benchSciPubmedId':'31461486'},{'sku':'10626D','imageId':'519381','imageUrl':'https://www.thermofisher.com/antibody/images/650/41467_2018_7006_Fig1_HTML-2-20190221105219.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/41467_2018_7006_Fig1_HTML-2-20190221105219.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/41467_2018_7006_Fig1_HTML-2-20190221105219.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/41467_2018_7006_Fig1_HTML-2-20190221105219.jpg?time\u003d20240507','sortOrder':'35','description':'Fig. 1 Characterization of exosomes from HIV-infected T cells. a Transmission electron microscope (TEM) images of exosomes isolated from T-cell culture supernatants. The representative J1.1 cell exosome image is shown. Scale bar, 100 nm. b Immunoblot of CD63, CD9, and CD81 on proteins extracted from T-cell line exosomes (whole scans of blots in Supplementary Figure 1). c CD63 and CD9 immunoblot (left) and AChE assays (right) of J1.1 exosomes isolated from 2, 10, and 20 ml of culture supernatants. Error bars, +- s.d. Data shown one experiment from three biological repeats. Numbers of exosomes (#Exo) were calculated by AChE activity using a standard provided by SBI','shortDescription':'Fig. 1 Characterization of exosomes from HIV-infected T cells. a Transmission electron microscope (TEM) images of exosomes isolated from T-cell culture supernatants. The representative J1.1 cell exosome image is shown. Scale bar, 100 nm. b Immunoblot of CD63, CD9, and CD81 on proteins extracted from T-cell line exosomes (whole scans of blots in Supplementary Figure 1). c CD63 and CD9 immunoblot (left)... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Nov 2, 2018, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Nature communications','journalText':'Nature communications 2018 - ','sourceType':'3','benchsci':true,'imageName':'41467_2018_7006_Fig1_HTML-2-20190221105219.jpg','benchSciPubmedId':'30389917'},{'sku':'10626D','imageId':'519380','imageUrl':'https://www.thermofisher.com/antibody/images/650/pone.0205496.g003-3-20190221105219.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/pone.0205496.g003-3-20190221105219.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/pone.0205496.g003-3-20190221105219.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/pone.0205496.g003-3-20190221105219.jpg?time\u003d20240507','sortOrder':'36','description':'Fig 3 Exosome isolation by the size exclusion chromatography. In (a) representative immunoblot showing the distribution of exosome markers (CD63, CD9, CD81) and high-abundance serum proteins (illustrated by Ponceau S staining) in the successive SEC fractions of a FaDU culture medium. In (b) total protein concentrations (mug/uL) in the subsequent SEC fractions. In (c) culture medium supplemented with 5% ED FBS and NOT co-cultured with cells was analyzed as in Panel A; \'\'E+\'\' denotes exosome-containing positive control.','shortDescription':'Fig 3 Exosome isolation by the size exclusion chromatography. In (a) representative immunoblot showing the distribution of exosome markers (CD63, CD9, CD81) and high-abundance serum proteins (illustrated by Ponceau S staining) in the successive SEC fractions of a FaDU culture medium. In (b) total protein concentrations (mug/uL) in the subsequent SEC fractions. In (c) culture medium supplemented with 5% E... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Apr 4, 2019, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'PloS one','journalText':'PloS one 2019 - ','sourceType':'3','benchsci':true,'imageName':'pone.0205496.g003-3-20190221105219.jpg','benchSciPubmedId':'30379855'},{'sku':'10626D','imageId':'735250','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_7513_pone.0204276.g006.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_7513_pone.0204276.g006.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_7513_pone.0204276.g006.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_7513_pone.0204276.g006.jpg?time\u003d20240507','sortOrder':'37','description':'Fig 6 Flow cytometry and WB analysis targeting the exosome marker CD9 on isolated vesicles. CD9 positive vesicles were detected by flow cytometry. Median fluorescence intensity (MFI) was reported as a signal to noise (S/N) ratio to isotype control in EVs isolated from E10 (A), BxPC3 (B) and H3 (C) cells (n \u003d 3). The presence of CD9 was also analyzed by WB, which was detected in vesicles from E10 (D) and BxPC3 cells (E) (n \u003d 3).','shortDescription':'Fig 6 Flow cytometry and WB analysis targeting the exosome marker CD9 on isolated vesicles. CD9 positive vesicles were detected by flow cytometry. Median fluorescence intensity (MFI) was reported as a signal to noise (S/N) ratio to isotype control in EVs isolated from E10 (A), BxPC3 (B) and H3 (C) cells (n \u003d 3). The presence of CD9 was also analyzed by WB, which was detected in vesicles from E10 (D) and BxP... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Mar 4, 2019, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'PloS one','journalText':'PloS one 2019 - ','sourceType':'3','benchsci':true,'imageName':'tfs_7513_pone.0204276.g006.jpg','benchSciPubmedId':'30260987'},{'sku':'10626D','imageId':'519379','imageUrl':'https://www.thermofisher.com/antibody/images/650/ZJEV_A_1456888_F0005_OC-1-20190221105219.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/ZJEV_A_1456888_F0005_OC-1-20190221105219.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/ZJEV_A_1456888_F0005_OC-1-20190221105219.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/ZJEV_A_1456888_F0005_OC-1-20190221105219.jpg?time\u003d20240507','sortOrder':'38','description':'Figure 5. Newt A1-EV characterization. (a) Dynamic light scattering was used to measure A1-EV size and concentration. The red error bars of the histogram indicate +/- one standard error of the mean size. Examples are shown of different A1-EV sizes by negative stain in electron micrographs (insets). (b) Acetylcholineesterase activity was measured for cell culture media (control) or isolated normal human dermal fibroblast (NHDF) EVs, cardiosphere-derived cell (CDC)-EVs, and the newt A1-EVs by fluorescent enzyme assays. (c and d) Graphical representation of the amount of RNA per particle (femtograms) and protein per particle (picograms) for NHDF-, CDC- and A1-EVs following the 3-day serum-starvation protocol of cultured cells. (e-h) Western blots of common EV surface proteins from A1 cells and EVs and mammalian NHDF cells and EVs are shown. This set of markers is comprised of thrombospondin1 (TSP1), periostin (POSTN), fibronectin (FN) and CD9. Example FACS bead assay histogram data with A1-EVs and NHDF-EVs stained to detect TSP1 (i), POSTN (j), FN (k) and CD9 (l). Quantitative measurements for abundance levels expressed as % of control antibody stains are indicated in green text for each FACS assay.','shortDescription':'Figure 5. Newt A1-EV characterization. (a) Dynamic light scattering was used to measure A1-EV size and concentration. The red error bars of the histogram indicate +/- one standard error of the mean size. Examples are shown of different A1-EV sizes by negative stain in electron micrographs (insets). (b) Acetylcholineesterase activity was measured for cell culture media (control) or isolated normal human derm... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Oct 1, 2020, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Journal of extracellular vesicles','journalText':'Journal of extracellular vesicles 2020 - ','sourceType':'3','benchsci':true,'imageName':'ZJEV_A_1456888_F0005_OC-1-20190221105219.jpg','benchSciPubmedId':'29696078'},{'sku':'10626D','imageId':'487253','imageUrl':'https://www.thermofisher.com/antibody/images/650/41598_2018_22450_Fig1_HTML-1-20190103084817.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/41598_2018_22450_Fig1_HTML-1-20190103084817.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/41598_2018_22450_Fig1_HTML-1-20190103084817.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/41598_2018_22450_Fig1_HTML-1-20190103084817.jpg?time\u003d20240507','sortOrder':'39','description':'Figure 1 EVs were purified from hiPSCs and non-hiPSCs (Table S1 ) by Tim4-immobilized beads. Purified EVs (0.2 mug) were electrophoresed under non-reducing conditions on 5-20% polyacrylamide gels and visualized with sliver staining. Separated proteins were transferred to a PVDF membrane and blotted with anti-CD9 and anti-CD63.','shortDescription':'Figure 1 EVs were purified from hiPSCs and non-hiPSCs (Table S1 ) by Tim4-immobilized beads. Purified EVs (0.2 mug) were electrophoresed under non-reducing conditions on 5-20% polyacrylamide gels and visualized with sliver staining. Separated proteins were transferred to a PVDF membrane and blotted with anti-CD9 and anti-CD63.','altTag':'CD9 Antibody in Western Blot (WB)','title':'CD9 Antibody (10626D) in WB','appAbv':'WB','dateTime':'Mar 5, 2018, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Scientific reports','journalText':'Scientific reports 2018 - ','sourceType':'3','benchsci':true,'imageName':'41598_2018_22450_Fig1_HTML-1-20190103084817.jpg','benchSciPubmedId':'29507392'},{'sku':'10626D','imageId':'1073857','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_27423_ZJEV_A_1807674_F0004_OC.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_27423_ZJEV_A_1807674_F0004_OC.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_27423_ZJEV_A_1807674_F0004_OC.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_27423_ZJEV_A_1807674_F0004_OC.jpg?time\u003d20240507','sortOrder':'40','description':'Figure 4. Analytical Size Exclusion Chromatography of hMSC-CM. (a) EV content of fractions evaluated by particle concentration measured by NTA i) (n \u003d 3 x 60s videos, N \u003d 3) and dot blots against CD63, CD81 and CD9 (N \u003d 3, quantified by Normalised Relative Intensity, N.R.I, as in methods). (a) ii) Representative dot blot images as used for quantification in a) i) (images re-cut to align with graph). (b) Total protein and VEGF concentrations of each fraction (VEGF measured on pooled adjacent pairs and plotted at mid-point). (c) i) Quantification of total tubule length in tubule formation assay for pooled adjacent fraction pairs (N \u003d 3, n \u003d 6, mean +- SD of n shown for each N (replicates 1-3, R1-3)). (c) ii) Representative whole-well images of tubule structures formed in C i). Images inverted for clarity. Scale bar \u003d 1 mm. (d) i) Quantification of wound closure rate for pooled adjacent fraction pairs (N \u003d 3, n \u003d 3, mean +- SD shown for each N (replicates 1-3, R1-3)). (d) ii) Representative images of scratch wounds for each fraction pair are shown for t \u003d 15 h (endpoint). Scale bar \u003d 100 mum. * and brackets: p \u003c 0.05, **: p \u003c 0.01, ***: p \u003c 0.001, ****: p \u003c 0.0001, (one-way within-subjects ANOVA and post-hoc Dunnett\u0027s test vs earliest measured fraction/s or negative control (NTA: F6, DB: F1, Total protein: F1, VEGF: F7-8, Tubule length: C-, Wound Closure Rate: C-)).','shortDescription':'Figure 4. Analytical Size Exclusion Chromatography of hMSC-CM. (a) EV content of fractions evaluated by particle concentration measured by NTA i) (n \u003d 3 x 60s videos, N \u003d 3) and dot blots against CD63, CD81 and CD9 (N \u003d 3, quantified by Normalised Relative Intensity, N.R.I, as in methods). (a) ii) Representative dot blot images as used for quantification in a) i) (images re-cut to align with graph). (b) Tot... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'CD9 Antibody in Dot Blot (DB)','title':'CD9 Antibody (10626D) in DB','appAbv':'DB','dateTime':'Aug 26, 2020, 4:00:00 AM','appName':'Dot blot','type':'Published Figures','journalTitle':'Journal of extracellular vesicles','journalText':'Journal of extracellular vesicles 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_27423_ZJEV_A_1807674_F0004_OC.jpg','benchSciPubmedId':'32944192'}],'Advanced Verification':[]}" image-displayed-id="'1111619'" product-sub-type="'antibody_primary'" prod-type="'antibody'" page-type="PDP" product-id="'10626D'"> <div class="tabs"> <ul class="nav nav-pills"> <li class="tab" onclick="window.THERMO_FISHER.inp.onTabClick(event, this)" data-tabname="Published Figures"> <a class="tab-text"> Published Figures (40) </a> </li> </ul> <div gallery-flt class="gal-flt" is-with-abbr="true" enable-mobile-flt="true" mobile-result-count="true" 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title="CD9 Antibody in Western Blot (WB)" longdesc="https://www.thermofisher.com/antibody/images/650/tfs_30047_IJO-63-3-05550-g04.jpg?time=20240507?time=20240507" onerror="javascript:imageErrorHandler.call(this, event);" > <div class="hover-text" ng-show="!$ctrl.isVideo"> <a href="javascript:return false;" onclick="window.THERMO_FISHER.inp.openImageGalleryModal(event, this)" aria-label="image-link"> <div class="rectangle"> <div class="expand-logo-rightcorner"> <?xml version="1.0" encoding="utf-8"?>  <svg width="19px" height="19px" version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px" viewBox="0 0 22 22" style="enable-background:new 0 0 22 22;" xml:space="preserve"> <style type="text/css"> .st0{fill-rule:evenodd;clip-rule:evenodd;fill:#222222;} </style> <title>Group 53</title> <desc>Created with Sketch.</desc> <g id="Symbols"> <g id="rollover-expand-icon" transform="translate(-8.000000, -8.000000)"> <g id="Group-48"> <g 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ng-show="!$ctrl.isVideo"> CD9 Antibody (10626D) in WB </h2> <div ng-show="!$ctrl.isVideo"> <div class="image-desc"> <div class="product-desc-text viewing-more-long-description"> <span class="image-journal-text" > International journal of oncology 2023 - </span> <span class="short-desc"> Morphology, size distribution, concentration and protein profiles of exosomes from HNSCC cell lines. Supernatants from HNSCC cell lines were collected and exosomes were isolated on mini-size exclusion chromatography columns. (A) Exosomes from UM-SCC-11B (11B), UM-SCC-14C (14C) and UM-SCC-22B (22B) show the typical vesicular shape and size in TEM images. Scale bar, 100 nm. (B) Western blotting was performed ... <a href="#" class="images-view-more-text" >View More</a> </span> <span class="verification-info hide" ng-if="$ctrl.hasVerificationMethod"> <a rel="nofollow" ng-href="{{$ctrl.currentElement.advancedVerification.uri}}" >{{ $ctrl.currentElement.advancedVerification.fullName }} validation info.</a> </span> <a href="#" class="images-view-more-text hide" ng-if="$ctrl.showViewMore" onclick="window.THERMO_FISHER.inp.openImageGalleryModal(event, this)"> View more </a> </div> </div> <div class="img-desc-footer"> <div class="benchsci-pubmed-footer"> <div ng-cloak ng-if="$ctrl.currentElement.benchsci || $ctrl.sourceType === '3'" class="images-benchsci-footer"> <div class="images-benchsci-link" tabindex="0"> <span>Published figure supplied by</span> <span class="icon-benchsci-logo"> <svg id="Layer_1" data-name="Layer 1" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 89.75 23.21"><defs><style>.cls-1{fill:#222222;}</style></defs><title>benchsci-logo</title><g id="logo-blue-copy-3"><g id="logo-blue-copy-2"><path id="path14" class="cls-1" d="M34.54,11.49a2.45,2.45,0,0,0,1.59-2.34c0-1.69-1.21-2.94-3.32-2.94H28.88v10.9h4.21a3.06,3.06,0,0,0,3.37-3A2.57,2.57,0,0,0,34.54,11.49ZM31,8h1.49c1,0,1.57.53,1.57,1.36a1.36,1.36,0,0,1-1.55,1.37H31Zm1.72,7.35H31V12.47h1.75a1.42,1.42,0,0,1,1.62,1.46C34.34,14.79,33.72,15.34,32.69,15.34Z"/><path id="path16" class="cls-1" d="M41.34,9.32a3.8,3.8,0,0,0-3.72,4,3.83,3.83,0,0,0,3.91,4.05A3.39,3.39,0,0,0,45,15l-1.71-.51a1.67,1.67,0,0,1-1.72,1.15,1.85,1.85,0,0,1-1.91-1.73H45s0-.34,0-.63C45.09,10.81,43.69,9.32,41.34,9.32Zm-1.66,3.15A1.63,1.63,0,0,1,41.37,11a1.52,1.52,0,0,1,1.69,1.49Z"/><path id="path18" class="cls-1" d="M50.58,9.35a2.48,2.48,0,0,0-2.18,1.14v-1h-2v7.57h2.05V12.75a1.42,1.42,0,0,1,1.41-1.57,1.34,1.34,0,0,1,1.4,1.52v4.42h2V12.35C53.32,10.68,52.46,9.35,50.58,9.35Z"/><path id="path20" class="cls-1" d="M58.2,15.47a1.94,1.94,0,0,1-1.94-2.14,1.92,1.92,0,0,1,1.91-2.12,1.64,1.64,0,0,1,1.67,1.27l1.83-.61a3.45,3.45,0,0,0-3.55-2.55,3.83,3.83,0,0,0-2.82,1.16,3.88,3.88,0,0,0-1.1,2.85,3.9,3.9,0,0,0,4,4,3.5,3.5,0,0,0,3.53-2.55l-1.8-.6A1.72,1.72,0,0,1,58.2,15.47Z"/><path id="path22" class="cls-1" d="M67,9.35a2.72,2.72,0,0,0-2.07.83V6h-2V17.12h2V12.67a1.43,1.43,0,0,1,1.42-1.48,1.34,1.34,0,0,1,1.4,1.53v4.41h2V12.35A2.67,2.67,0,0,0,67,9.35Z"/><path id="path24" class="cls-1" d="M75.82,11.05l-1.63-.35a1.58,1.58,0,0,1-1.4-1.59A1.93,1.93,0,0,1,74.9,7.3a2.21,2.21,0,0,1,2.3,1.86l1.36-.48a3.73,3.73,0,0,0-7.29.54,3,3,0,0,0,2.67,3l1.55.33c1.09.24,1.63.91,1.63,1.7,0,.95-.74,1.74-2.25,1.74a2.51,2.51,0,0,1-1.79-.63,2.45,2.45,0,0,1-.82-1.71l-1.45.46a3.79,3.79,0,0,0,4.08,3.24c2.32,0,3.76-1.52,3.76-3.22C78.65,12.59,77.63,11.45,75.82,11.05Z"/><path id="path26" class="cls-1" d="M83.25,16A2.33,2.33,0,0,1,81,13.41a2.31,2.31,0,0,1,2.24-2.59,1.93,1.93,0,0,1,2,1.57l1.31-.55a3.22,3.22,0,0,0-3.29-2.35,3.7,3.7,0,0,0-3.72,3.92,3.75,3.75,0,0,0,3.74,3.94A3.41,3.41,0,0,0,86.59,15l-1.28-.55A2,2,0,0,1,83.25,16Z"/><path id="path28" class="cls-1" d="M88.69,5.89a1,1,0,1,0,.75.29A1,1,0,0,0,88.69,5.89Z"/><polygon id="rect30" class="cls-1" points="87.99 9.72 89.42 9.72 89.42 17.11 87.99 17.11 87.99 9.72"/><path id="path32" class="cls-1" d="M14.58,8.87A4.1,4.1,0,0,0,15.8,6H14.31a2.62,2.62,0,0,1-.76,1.87L11.66,9.7l-2-2A2.61,2.61,0,0,1,9,6H7.53A4.09,4.09,0,0,0,8.74,8.87l1.87,1.87L8.74,12.61a4.08,4.08,0,0,0-1.21,2.92v2.29H9.18V16.73h1.66v1.09h1.64V16.73h1.65v1.09H15.8V15.53a4.11,4.11,0,0,0-1.21-2.92l-1.38-1.36-1,1.05,1.37,1.36a2.63,2.63,0,0,1,.77,1.58H9a2.68,2.68,0,0,1,.76-1.58Z"/><path id="path34" class="cls-1" d="M11.61,23.21a11.61,11.61,0,1,1,11.6-11.6A11.6,11.6,0,0,1,11.61,23.21Zm0-21.89A10.29,10.29,0,1,0,21.9,11.61,10.28,10.28,0,0,0,11.61,1.32Z"/></g></g></svg> </span> <span id="bechsci-tooltip-{{$ctrl.productId}}" class="bechsci-tooltip-icon" data-toggle="tooltip" ng-click="$ctrl.showBechSciTooltip()" close-tooltip="$ctrl.closeBechSciTooltip()" data-html="true" title="Clicking this link will redirect you to the <a href='https://www.benchsci.com/' target='_blank'> BenchSci</a> website that provides third-party scientific content. 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src="https://www.thermofisher.com/antibody/images/150/pone.0205496.g003-3-20190221105219.jpg?time=20240507" alt="CD9 Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="735250" data-index="36"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_7513_pone.0204276.g006.jpg?time=20240507" alt="CD9 Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="519379" data-index="37"> <img src="https://www.thermofisher.com/antibody/images/150/ZJEV_A_1456888_F0005_OC-1-20190221105219.jpg?time=20240507" alt="CD9 Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="487253" data-index="38"> <img src="https://www.thermofisher.com/antibody/images/150/41598_2018_22450_Fig1_HTML-1-20190103084817.jpg?time=20240507" alt="CD9 Antibody in Western Blot (WB)"/> </div> <div image-gallery-fade-server-side class="img-flipcard" onclick="window.THERMO_FISHER.inp.onClickFilmstripItem(event, this)" data-imgid="1073857" data-index="39"> <img src="https://www.thermofisher.com/antibody/images/150/tfs_27423_ZJEV_A_1807674_F0004_OC.jpg?time=20240507" alt="CD9 Antibody in Dot Blot (DB)"/> </div> </div> </div> </div> </div> <noscript> <div itemscope itemtype="http://schema.org/ImageGallery"> <h2>Product Image Gallery</h2> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_30047_IJO-63-3-05550-g04.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Morphology, size distribution, concentration and protein profiles of exosomes from HNSCC cell lines. Supernatants from HNSCC cell lines were collected and exosomes were isolated on mini-size exclusion chromatography columns. (A) Exosomes from UM-SCC-11B (11B), UM-SCC-14C (14C) and UM-SCC-22B (22B) show the typical vesicular shape and size in TEM images. Scale bar, 100 nm. (B) Western blotting was performed after loading 10 u g exosome preparation per lane and show the exosome markers TSG101, CD63 and CD9 and ApoA1 and Grp94 were used as purity control. (C) Nanoparticle tracking analysis was performed to detect median sizes and particle concentrations of the isolated particles. Representative pictures are shown of representative exosome preparations. HNSCC, head and neck squamous cell carcinoma.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_27424_fphys-12-630933-g002.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">FIGURE 2 Particle population characteristics, zeta potential, and western blot analysis of cell-derived EVs. (A) Categorization of EV populations by size distribution by TRPS. The majority of EVs belong to a size range of 100-250 nm. t-test was performed between their counterparts. (B) pH affects the zeta potential of EVs: Compared to physiological pH (7.4), cyst microenvironmental pH (6.0) showed a drastic change in the zeta potential (measured by TRPS) of EVs derived from T1G cells. (C) Western blot analysis of EVs. Classical EV markers such as Alix, TSG 101, ARL13b, CD63, CD81, and CD9 were present in EVs derived from mIMCD3 and T1G cells. Data ( n = 3 ) were represented as mean +- SD. Significant difference analyzed by t -test (* p < 0.05, ** p < 0.01, *** p < 0.001).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_9657_sensors-20-00965-g006.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Immunocytochemistry (ICC/IF)" title="CD9 Antibody (10626D) in ICC/IF"> <figcaption itemprop="caption">Figure 6 Confocal microscopy to evaluate the relative expression of CD9, CD63, CD81, CD24, CD44, CD54, CD326 and CD340 membrane protein markers in the exosomes derived from MCF7, MDA-MB-231 and SKBr3 breast cancer cell lines. Magnetic particles appear stained in green while the membrane protein receptors, in red, represents a positive expression on the membrane of the exosomes. In all cases, the primary antibody was 5 mug mL -1 and 2 µg mL -1 of antimouse-Cy5 antibody.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_30062_41232_2023_299_Fig5_HTML.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Collection of small extracellular vesicles (sEVs) by ultracentrifugation (UC) and tangential flow filtration (TTF). A Particle size distribution of sEVs when collected by UC and TFF. B Particle number collected from the same volume (200 ml) of mesenchymal stem cell (MSC) culture supernatant. Western blot analysis of CD9 ( C ), and CD81 ( D ) of sEVs collected using UC and TFF. n = 3 per experiment. *** p < 0.001, Mann-Whitney U analysis</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_29696_ijms-24-08506-g002.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Molecular characterization of EVs from human A375 melanoma cells and human M12 melanoma brain metastases cells. ( a ) Schematic representation of samples. ( b ) Western blot images of intracellular vesicle contaminant marker calnexin and EV cytosolic (ALIX, TSG101, and annexin V) and membrane (CD63, CD9, and CD81) markers.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_29674_10020_2023_659_Fig1_HTML.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Characterization of plasma sEVs. sEVs were freshly isolated from plasma of HNC, NED patients and HD by size-exclusion chromatography and characterized for their morphology, particle concentration and protein content. A Representative TEM images of sEVs from HNC, NED patients and HD are shown (magnification = 25.000x, scale = 100 nm). B Size distribution, concentration and median diameter of the particles were detected using NTA. C NTA particle concentration of HNC, NED patients and HD were visualized by scatter plots. D Comparison of total protein content in 1 mL of isolated sEVs measured by BCA protein assays. E Western blots were performed using 10 mug protein and the presence of typical vesicle proteins CD9, CD63 and TSG101 and the absence of contaminating proteins (Grp94, ApoA1) were shown. P values below 0.05 were considered as significant (*p < 0.05)</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_27422_fphys-14-1143966-g002.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">FIGURE 2 Characterization of myotube derived extracellular vesicles (EVs). Human myotubes were exposed to electrical pulse stimulation (EPS) for 24 h, and cell derived EVs were collected for 24 h thereafter. Size (A) and concentration (C) of exosomes (EXO) and microvesicles (MV) were measured by nanoparticle tracking analysis (NTA). The presence of EV markers on exosomes and MV captured by anti-CD81-coated magnetic beads were detected with PE-conjugated CD81 (B) and CD63 (D) antibodies by flow-cytometry (BD Accuri C6 flow cytometer). Presence of CD9, calnexin, and heat shock protein 70 (Hsc/Hsp70) on exosomes were measured by Western blotting (E) . Cell lysates of SW480 cells (SW) were used as control. Transmission electron microscopy (TEM) images of skeletal muscle cell derived EVs (F) . 1. Freshly isolated EVs from conditioned media from human myotubes, scale bar 1 um. 2. Close up of framed EVs in picture 1, scale bar 200 nm. Data are presented as mean +- SEM ( n = 6 in each group). MFI = mean fluorescence intensity.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_27421_pharmaceutics-15-00953-g001.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Characterization of platelets and isolated platelet-derived extracellular vesicles (pEV). ( A ) Schematic overview of the density gradient ultracentrifugation (DGUC) protocol used to isolate pEV from expired platelet concentrates (PC). ( B ) Expression of platelet activation marker CD62p in basal and thrombin-stimulated platelets ( n = 3; two-tailed unpaired t -test, *** p < 0.001). ( C ) Representative transmission electron microscopy (TEM) micrographs of platelets from the expired PC (activated platelets are indicated by black arrows and resting platelets by red arrows). Scale bars: 1 mum. ( D ) Western blot analysis of specific EV markers (tetraspanins CD63 (glycosylated form) and CD9, and cytosolic protein flotillin-2), platelet-specific marker (CD41), and non-EV markers [apolipoprotein (ApoA1) and argonaute 2 (Ago2)] in pooled fractions and platelet lysate (PLTS). Molecular weight (MW) markers are indicated. ( E ) Representative TEM images of negatively stained 8-9 fractions, enriched in cup-shaped pEV (black arrows). Higher magnification image detailing the morphology of pEV. Scale bars: 1 um and 200 nm (high magnification). ( F ) Representative size distribution profiles of 8-9 pooled pEV-fractions analyzed using nanoparticle tracking analysis. Size distribution is represented as mean (black continuous line) +- standard deviation (shaded area).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_27420_fimmu-14-1107150-g001.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Characterization of exosomes. (A) Concentration and size distribution of exosomes isolated from patients and healthy volunteers' plasma, nanoparticle tracking analysis (NTA); (B) CD63, CD81 and CD9 expression in plasma-isolated exosomes from healthy volunteers (Lines 1-4) and polytrauma patients (Lines 5-7), Representative Western-blot analysis.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_21105_12964_2022_863_Fig1_HTML.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Characterization of human oligodendroglia-derived extracellular vesicles. A Western blotting analysis of small extracellular vesicle protein markers CD63, CD9, Flotillin-1, Annexin A2, HSP70, and the negative control marker GRP94. B Representative overview (scale bar 200 nm) and cropped (scale bar 100 nm) image showing the morphology, as revealed by transmission electron microscopy, of EVs isolated from wild-type native human oligodendroglia (OL-EVs) and stably expressing HSPB8 oligodendroglia (OL-HSPB8-EV). C - E Particle size profile distribution, particles per mL, and total EV protein measured by Nanoparticle Tracking Analysis (NTA) and BCA of OL-EVs and OL-HSPB8-EVs, pelleted from 30 mL of conditioned medium. F - H Western blotting and qPCR analysis of HSPB8 present in OL-EVs and OL-HSPB8-EVs. Statistical analysis was performed using GraphPad. Student's t-test was used to determine significance (** P < 0.01, **** P < 0.0001). Data are presented as mean +- SEM</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_20099_41598_2021_1334_Fig2_HTML.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Figure 2 Western blot detection of CD9, Hsc70/Hsp70 and Calnexin in plasma pool EVs. As a positive control, lysate from the colorectal cancer cell line SW480 was used. Full-length blots are presented in Supplementary Fig. 3 (Supplementary Information).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_20030_41467_2021_26485_Fig5_HTML.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Fig. 5 MAAP promotes association of AAV with EVs. A Schematic of EV isolation by iodixanol density gradient from HEK293 suspension culture. B Immunoblots of iodixanol fractions from suspension cells producing recombinant MAAP8Delta vector complemented in trans with CMV-HA and C CMV-MAAP8-HA. EVs, capsid and MAAP were analyzed from the media of HEK293 producing cells at day 3 post transfection. Capsid and MAAP proteins were analyzed by SDS-PAGE under reducing conditions while EV markers (CD81, CD63, CD9) were analyzed by SDS-PAGE under non-reducing conditions ( n = 2). D Graph displaying percent vector genome titer relative to total viral genomes for each fraction from iodixanol gradient purified MAAP8Delta vector complemented in trans with CMV-HA and CMV-MAAP8-HA. E Schematic of EV isolation by size exclusion chromatography (SEC) from HEK293 suspension culture. F Immunoblots of SEC fractions from suspension cells producing recombinant MAAP8Delta vector complemented in trans with CMV-HA and G CMV-MAAP8-HA. EVs and MAAP were analyzed from the media of HEK293 producing cells at day 3 post transfection. MAAP protein was analyzed by SDS-PAGE under reducing conditions while the EV marker CD63 was analyzed by SDS-PAGE under non-reducing conditions ( n = 2). H Graph displaying percent vector genome titer relative to total viral genomes for each fraction from SEC purified MAAP8Delta vector complemented in trans with CMV-HA and CMV-MAAP8-HA.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_17750_fimmu-12-732209-g002.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Figure 2 HR promoted the concentration of EVs and CD203c + -EVs and CD63 + -EVs derived from serum. 500mul serum from control and different time point HR groups were used to pellet EVs, finally, the EVs were resuspended in 200mul PBS. The total concentration of EVs was determined by a BCA assay. The protein expression levels of CD63, CD203c, CD9 and CD81 in EVs/serum were identified using western blotting. (A) Total concentration of EVs isolated from same volume serum of control and different time point HR groups, n = 10. (B-F) Relative CD63, CD203c, CD9 and CD81 protein expression levels of EVs derived from same volume of serum in control and HR groups, 30mul EVs/lane, n = 5 (G-I) Relative CD63 and CD203c expression levels in same volume of serum in the control and HR groups, 30mul serum/lane, n = 5. All values represent the mean +- standard deviation. Difference to control: ** p < 0,01; *** p < 0,001; # Difference between groups: # p < 0,05; ## p < 0,01; 1 way ANOVA with Newman-Keuls Multiple Comparison Test.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_17750_fimmu-12-732209-g001.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Figure 1 Characterization of control EVs and HR-EVs. EVs were processed for western blot, TEM and NTA after isolated from 500mul serum of control and HR groups. (A) The same amount (30mul) of control EVs, HR-EVs and EVs-depleted-Serum were identified using western blot. Representative western blot image showing bands of standard surface markers (CD9, CD63, CD81) of control EVs and HR-EVs. (B, C) Morphology of control EVs and HR-EVs was monitored by TEM; Scale bar: 100 nm. (D, E) Particle size distribution of control EVs and HR-EVs was determined by NTA. Distribution of EVs with a size of 90-200 nm in diameter in both groups. (F, G) Quantitative comparison between control EVs and HR-EVs in count and size measured by NTA; n = 10; All values represent mean +- standard deviation. * p < 0,05, independent two-tailed Student's t -tests.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_15302_mmr-24-05-12455-g04.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Figure 5. Release of exosomes from Mtb H37Rv-infected or naive THP-1 cells. THP-1 macrophages were infected with Mtb H37Rv (Mtb + ) for 4 h (MOI 5) in DCM-UG medium for 4, 24 and 48 h at 37degC and in the presence of 5% CO 2 . Exosomes were extracted from cell culture supernatants using total exosome isolation reagent and were further analyzed by western blot analysis for the exosomal protein markers, (A) CD63, CD81, CD9 and (B) LAMP-1, which served as a positive control for exosomes signal and Mtb proteins [Ag85 and Mpt64 and recombinant Mtb Mpt64 protein (His-tag)]. Data from one representative experiment out of at least three independent experiments are shown. MOI, multiplicity of infection; Mtb, Mycobacterium tuberculosis ; DCM-UG, ultra-centrifuged CellGenix (r) GMP DC Medium; LAMP-1, lysosomal associated membrane protein-1; Ag85, mycobacterial antigen 85.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_17054_cancers-13-04176-g001.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Figure 1 Characterization of small extracellular vesicles (sEVs) derived from human Head and Neck Squamous Cell Carcinoma cell lines: FaDu (hypopharyngeal squamous cell carcinoma), PCI-30 (tongue squamous cell carcinoma), SCC-25 (tongue squamous cell carcinoma); and sEVs derived from HaCat (immortalized human keratinocytes). ( A ) Representative cryogenic transmission microscopy image of HNSCC cell-derived sEVs. ( B ) Representative concentration and size distribution plot of HNSCC-derived sEVs measured by nanoparticle tracking analysis (NTA) and particle visualization based on Brownian motions. ( C ) Immunoblotting of sEV markers CD63, CD9, and negative marker Grp94 in HNSCC-derived sEVs. Full blot images are presented in Figures S2-S4 . ( D ) Particle concentration related to normoxic (21% O 2 ) and hypoxic (1% O 2 , 5% O 2 , 10% O 2 ) conditions. Results were obtained using NTA and normalized to the protein levels in lysates of producer cells. ( E ) Particle diameter related to normoxic (21% O 2 ) and hypoxic (1% O 2 , 5% O 2 , 10% O 2 ) conditions. Results were obtained using NTA. All data represent three biological replicates and are presented as means +- SD. * p < 0.05 vs. 21%; ** p < 0.01 vs. 21%; *** p < 0.001 vs. 21%.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_17590_41419_2021_4069_Fig1_HTML.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Fig. 1 Characterization of tumor microvesicles. A Particle volume distribution in function of sizes of plasma tumor MVs (T-MVs) from giant cell tumor of bone patient 1, healthy control microvesicles (C-MVs), T-MVs from patient 2, and patient 3, as indicated. B Distribution of particle concentrations in function of sizes in a representative preparation of plasma T-MVs determined by nanoparticle-tracking analysis (NanoSight). Histogram of typical preparations containing 10 9 T-MVs/ml. C Flow cytometry scatter plot of T-MVs showing autofluorescence settings and staining with 7-AAD, indicating high integrity of isolated MVs. D Representative fluorescence activated cell sorting (FACS) analysis of healthy subjects' control MVs (C-MVs) and T-MVs detected by CD63 and CD9 antibodies and cytometric analysis of CD44, and CD117 antigen expression in T-MVs of different patients. Percent of positive MVs are indicated in each quadrant. E Western blots of 30 ug protein extracts from isolated MVs. Lanes C1-C3; proteins from healthy control microvesicles (C-MVs). Lanes T1-T7, proteins from tumor patient microvesicles (T-MVs). Specific antibodies used for immunoblots are indicated. F Upper panel: red PONCEAU blots of 30 ug protein extracts from isolated MVs. Lane M; marker, Lanes C1-C3; proteins from healthy control microvesicles (C-MVs). Lanes T1-T7, proteins from tumor patient microvesicles (T-MVs). Lower panel western blot for Cytochrome C determination (negative control). Tubulin was used a</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_16901_fmolb-08-685088-g002.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">FIGURE 2 Characterization of control EVs and gene analysis of hsa-124-3p in serum-derived EVs and serum. (A) Representative image of Western blotting showed bands of standard surface markers ( CD9 , CD63 , and CD81 ) of EVs and EV-depleted serum. (B) Morphology of EVs was monitored by TEM; scale bar: 100 nm. (C,D) Particle size distribution of EVs was determined by NTA. (E,F) Gene expression of hsa-miR-124-3p in EVs and serum of different samples at different time points. Compared to the control group: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001, # Difference between groups: # p < 0.05; ## p < 0.01; #### p < 0.0001; one-way ANOVA with the Newman-Keuls multiple comparison test.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_14108_13287_2021_2317_Fig3_HTML.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Fig. 3 Characterization of control EVs and Cur-EVs. a Representative western blot image showing bands of standard surface markers (CD9, CD63, CD81) of control EV- and Cur-EV lysates. b , c Morphology of control EVs and Cur-EVs was monitored by TEM; scale bar, 100 nm. d , e Particle size distribution of control EVs and Cur-EVs was measured by NTA. f , g Quantitative comparison between control EVs and Cur-EVs in count and size measured by NTA; n = 3. h Absorbance at 420 nm of control EVs and Cur-EVs was determined with by spectrophotometry indicating presence of curcumin in Cur-EVs; n = 3. i Cell nuclei were stained with DAPI (blue) and chondrocytes were stained with Phalloidin (green) to visualize the structure of the cytoskeleton. PKH26-labeled control EVs (red) and Cur-EVs (red) were internalized by chondrocytes and visualized with fluorescent microscopy</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_14078_pharmaceuticals-14-00356-g001.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Figure 1 Characterization of extracellular vesicles (EVs) loaded with CRISPR associated protein 9 (Cas9). ( a ) Schematic of loading procedure. The conditioned medium of MDA-MB-231 human breast cancer cells was collected (step 1) and tangential flow filtration (TFF) was performed to remove components larger than 650 nm and smaller than 500 kDa (step 2). The isolated EVs were gently mixed with PULSin/Cas9 (step 3) and size-exclusion chromatography (SEC) was used to remove free Cas9, PULSin and Cas9/PULSin (step 4). ( b ) Mean size determined by nanoparticle tracking analysis (NTA). ( c ) Zeta potential measured by laser Doppler micro-electrophoresis. ( d ) Cas9 loading and protein markers of EVs, heat-shock cognate protein 71 (Hsc70), cluster of differentiation (CD)63, CD9 and annexin V, and intracellular contaminant marker, calnexin, detected by Western blot. Data are presented as mean +- SD of three biological replicates (b and c). Statistical analysis was performed by one-way analysis of variance (ANOVA) with post-hoc pairwise comparisons using Tukey''s test. *, p < 0.05; ****, p < 0.0001.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_19715_fbioe-08-615520-g005.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">FIGURE 5 EV characterization and validation. (A) Representative SEM pictures of BMSC EVs. White arrows label EVs with 97 nm (left image) and 115 nm (right image) diameter. Magnification: 60,000x. (B) Representative TEM pictures of BMSC EVs. Scale bar left image = 200 nm, magnification: 100,000x. Right image shows magnification of the EV in the green box. The size of objects was determined (red lines). Vertical line = 104 nm, horizontal line = 113 nm. (C) Western Blot analysis of EV specific surface markers CD9 (left image) and CD81 (right image) in BMSC, CA, CA/OP, and OP EVs and in the EV-depleted FCS depl - uc . Lane 1 = MW ladder, Lane 2 = 5 mug; Lanes 3-6 = 8.2 mug; Lane 7 = 10 mul; Exposure time: left image = 3 min, right image = 10 min (Pierce femto Kit). For respective Ponceau Red images, see Supplementary Figure 3 . (D) Test for EV uptake of BMSC-derived EVs into cultured BMSCs using PKH-26 (red) stained EVs (lower panel). PBS solution + PKH-26 stain was used as negative control (upper panel). Nuclei were counterstained with DAPI. Scale bar 100 mum.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_19715_Image_3.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_19719_fbioe-08-603598-g0003.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Figure 3 Characterization of EV-depleted FCS (FCS depl-uc ). Prior to ultracentrifugation, normal FCS was diluted in culture medium to different concentrations (20, 50%, and undiluted = 100%); different FCS depl-uc groups were identified after ultracentrifugation. (A,B) FCS depl-uc-20% was controlled for EV surface makers (CD9, CD63, and CD81) detected by western blotting (lane 1: hBMSC lysate; lane 2: hBMSC-EVs; lane 3: undepleted FCS; lane 4: FCS depl-uc-20% ). Representative western blot image (A) and Ponceau Red-stained images for each surface marker (B) are shown; n = 3. (C,D) Proliferation and apoptosis of hBMSC were determined by BrdU assay and caspase-3/7 activity assay separately after being incubated in culture medium supplemented with the different FCS groups for 24 h. All values represent mean +- standard deviation. *Significant difference to control: * p < 0.05; ** p < 0.01; # Significant difference between groups: # p < 0.05; ### p < 0.001 one-way ANOVA with Newman-Keuls Multiple Comparison Test; n = 4.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_19719_fbioe-08-603598-g0002.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Figure 2 Characterization of hBMSC-derived EVs. (A) Representative western blot image ( n = 4) demonstrates standard surface markers (CD9, CD63, and CD81) of hBMSC-derived EVs (lane 1: hBMSC lysate; lane 2: hBMSC-EVs). (B) Particle size distribution of hBMSC-EVs was measured by NTA. (C) Morphology of hBMSC-EVs was monitored by SEM, scale bar: 1 mum. (D) Cell nuclei were stained with DAPI (blue) and chondrocytes were stained with phalloidin (green) to visualize the cytoskeleton. PKH26-labeled hBMSC-derived EVs (red) internalized by chondrocytes were visualized with fluorescent microscopy.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_19657_IJMM-46-06-2115-g00.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Figure 1 Characterization of OCEXs. (A) The sizes of the WSU-HN4- and SCC-9-derived exosomes were determined by NTA. (B) Representative images of exosomes derived from WSU-HN4 and SCC-9 cells, as detected by TEM. Scale bars, 50 nm. (C) Expression of the exosomal markers, Alix, CD63, CD9 and Rab5, in WSU-HN4- and SCC-9-derived exosomes was determined by western blot analysis. (D) Co-expressed proteins in WSU-HN4- and SCC-9-derived exosomes were detected by mass spectrometry. (E) Overlap of OCEX proteins and the top 100 frequently identified exosomal protein markers from the ExoCarta database. OCEXs, oral cancer-derived exosomes; NTA, nanoparticle tracking analysis; TEM, transmission electron microscopy; EXO/Exo, exosomes.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_11761_pone.0238591.g001.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Fig 1 Characterization of the small EVs isolated from the cell culture supernatant of the E10, BxPC3, and H3 cell lines. EVs were studied by protein concentration measurements (A), NTA for particle quantification (B), immunoaffinity capture targeting CD9 (C), and Western blot for the detection of CD9 in three sequential SEC fractions (20 mul per well) (D). Samples were negatively stained with 4% uranyl acetate (aqueous) for analysis by TEM (E). Data republished from Guerreiro et al [].</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_9770_cells-09-01946-g004.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Figure 4 Protein markers of plasma-derived EVs. ( a ) Protein concentration for each sample normalized to 1 mL of original plasma. ( b ) Western blot of cluster of differentiation 9 (CD9) (EV marker) and calnexin (contaminant marker). P is plasma. ( c ) Enzyme linked immunosorbent assay (ELISA) for apolipoprotein B (apoB). Data represent mean +- s.d. ( n = 3) Statistics by one-way analysis of variance (ANOVA). * p < 0.025; ** p < 0.004; *** p < 0.0007; ****, p < 0.0001; ns, not significant.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_11647_fgene-11-00712-g004.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">FIGURE 4 Positive detection for specific exosomal surface markers (CD 63, CD9, and CD81) and for the control-positive marker (b-actin) by immunoblotting. (A) exosomes isolated by UC and (B) TEIp kit.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_11746_cancers-12-02110-g002.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Figure 2 Successful isolation of exosomes from blood plasma was verified by Transmission Electron Microscopy (TEM), Western Blot, and Nanoparticle tracking. ( A ) Two representative TEM graphs showing negatively stained exosomes isolated from an HNSCC patient. As indicated by the size bars, exosomes vary in diameter between 30 and 150 nm and have round to oval shapes. Size bar on the top TEM graph = 500 nm, size bar on the bottom TEM graph = 200 nm. ( B ) Western Blot analysis of exosomes was performed to confirm the expression of exosomal markers TSG101, CD9 and CD63 and the expression of epithelial cell marker EpCAM (upper frame). Exosomes were also analyzed for negative markers ApoA1 and Grp94 along with plasma (diluted 50x in PBS) and cell lysate samples as positive controls. MW marker, positive control molecular weight marker. ( C ) Size distribution of exosomes was measured by nanoparticle tracking. The mean diameter was 86.8 nm. The maximal and minimal diameters were 257.5 nm and 22.5 nm, respectively. The 90th and 10th percentile were at 121.2 and 53.6 nm, respectively. ( D ) Protein content of exosomes was determined by Bicinchoninic Acid (BCA) Assay. Average protein content: 80.9 g/mL (HNSCC exosomes), 69.2 g/mL (healthy volunteer exosomes). n = 23 (HNSCC), n = 10 (NC). HNSCC, exosomes from blood plasma of HNSCC patients. NC = no cancer, exosomes from blood plasma of healthy volunteers. ( E ) B cells that were not co-cultured with exosomes exhibited colony formation</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_13727_ijms-21-03739-g001.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Figure 1 Characterization of exosomes isolated from plasma. ( A ) Representative transmission electron microscopy (TEM) image of exosomes. Scalebar = 200 nm. ( B ) Representative size distribution of exosomes measured by nanoparticle tracking analysis (NTA). ( C ) Exosomes derived from plasma of healthy donors (HD) and head and neck squamous cell carcinoma (HNSCC) patients were analyzed by Western blot for the presence of exosome specific markers using antibodies against CD63 and CD81 under non-reducing conditions and antibodies against CD9 and TSG101 under reducing conditions. Western blot analysis for the negative marker Grp94 and the apolipoprotein Apo1A was also performed for exosomes, cells, and plasma.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_12307_18_2020_3507_Fig3_HTML.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Fig. 3 Effect of metalloproteinase inhibitors on ADAM10 internalization, degradation, and release in microvesicles. a , b THP-1 cells were treated with 10 muM GI or vehicle control. After 4 h ( a ) or 2 h ( b ), cells were analyzed for surface expression of ADAM10 by flow cytometry. The geometric mean fluorescence of treated cells was calculated in relation to that of the respective control and summarized as mean and SD of three independent experiments.) THP-1 cells were treated with 10 muM GI or vehicle control for 16 h in the absence or presence of 40 mM NH4Cl ( c ) or 0.5 muM bafilomycin A1 ( d ). Cell lysates were probed by western blotting with antibodies against the N-terminus of ADAM10 or against GAPDH as loading control. Data are shown as representative western blot and as relative changes of band intensity determined by densitometric analysis of three independent experiments. e-f THP-1 cells were treated with 10 muM GI, 10 muM TAPI or vehicle control for 24 h. Extracellular vesicles (EV) were prepared from conditioned cell media by differential centrifugation. Lysates and EV preparation were then subjected to western blot analysis with antibodies against the N-terminus of ADAM10, against the exosomal marker CD9 or against GAPDH as internal control ( e ). EV preparations from conditioned media were conjugated to beads which were then studied for ADAM10 immunoreactivity by flow cytometry ( f ). Data are shown as representative result or as means and SD of four independ</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_11322_41598_2020_62920_Fig1_HTML.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Figure 1 ( a ) Nanoparticle Tracking analysis (NTA) of EVs derived from CRL-5908 cells isolated with three different methods: Yellow line indicates EVs isolated with one-step ultracentrifuge method, red line EVs isolated with commercial kit, and blue line EVs isolated with double-step ultracentrifuge method (peak indicated by arrow). EVs have a mean diameter of 133.7 +/- 6.5 nm and mode of 107.5 +/- 1.7 nm. EV-concentration is expressed as numbers of particles per mL, y axis is marked from 1 to 3,5 E10. ( b ) Western-blot image of CRL-5908 and CCL-185 cells lysates and their respective isolated EVs: EVs lysates showed higher expression of CD9, lower but presence of HSP70, and absence of GM130 in comparison with cell lines lysates.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_7931_biomolecules-10-00150-g002.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Figure 2 Extracellular vescile (EV) characterization. ( A ) Nanoparticle tracking analysis (NTA) showing a peak between 100-200 nm for the control (CT), luminal A (LA), and triple negative (TNBC) groups. ( B ) Transmission electron microscopy (TEM) image of EVs from cancer patient showing a size corresponding to NTA results. Size bar = 200 nm. ( C ) Western blotting (WB) analysis showing strong protein expression of CD9 and CD63 on EVs from control (2) and cancer (4), when compared with corresponding serum supernatant EV-depleted from control (1) and cancer (3).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_6533_pone.0221679.g001.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Fig 1 Morphological and biochemical analysis of isolated CAP exosomes. ( A ) Transmission electron microscopy pictures of parental (left) and modified (right) CAP exsomes. Scale bar represents 100 nm. ( B ) Western blot of 30 mug cellular and exosomal protein lysates per lane. Blotting performed on GRP78 (upper panel), turbo-GFP (middle panel) and CD9 protein (lower panel). Lane 1-4 represent cellular lysates, while lanes 5-8 depict exosomal lysate. ( C ) Quantification of Western blot Fig 1 B for CD9 signal. ( D ) Quantification of Western blot Fig 1 B for tGFP signal. ( E ) Quantification of Western blot Fig 1 B for GRP78 signal. ( F ) Flow cytometry analysis of GFP-positive exosomes isolated from parental and modified CAP cells.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/41467_2018_7006_Fig1_HTML-2-20190221105219.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Fig. 1 Characterization of exosomes from HIV-infected T cells. a Transmission electron microscope (TEM) images of exosomes isolated from T-cell culture supernatants. The representative J1.1 cell exosome image is shown. Scale bar, 100 nm. b Immunoblot of CD63, CD9, and CD81 on proteins extracted from T-cell line exosomes (whole scans of blots in Supplementary Figure 1). c CD63 and CD9 immunoblot (left) and AChE assays (right) of J1.1 exosomes isolated from 2, 10, and 20 ml of culture supernatants. Error bars, +- s.d. Data shown one experiment from three biological repeats. Numbers of exosomes (#Exo) were calculated by AChE activity using a standard provided by SBI</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/pone.0205496.g003-3-20190221105219.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Fig 3 Exosome isolation by the size exclusion chromatography. In (a) representative immunoblot showing the distribution of exosome markers (CD63, CD9, CD81) and high-abundance serum proteins (illustrated by Ponceau S staining) in the successive SEC fractions of a FaDU culture medium. In (b) total protein concentrations (mug/uL) in the subsequent SEC fractions. In (c) culture medium supplemented with 5% ED FBS and NOT co-cultured with cells was analyzed as in Panel A; ""E+"" denotes exosome-containing positive control.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_7513_pone.0204276.g006.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Fig 6 Flow cytometry and WB analysis targeting the exosome marker CD9 on isolated vesicles. CD9 positive vesicles were detected by flow cytometry. Median fluorescence intensity (MFI) was reported as a signal to noise (S/N) ratio to isotype control in EVs isolated from E10 (A), BxPC3 (B) and H3 (C) cells (n = 3). The presence of CD9 was also analyzed by WB, which was detected in vesicles from E10 (D) and BxPC3 cells (E) (n = 3).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/ZJEV_A_1456888_F0005_OC-1-20190221105219.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Figure 5. Newt A1-EV characterization. (a) Dynamic light scattering was used to measure A1-EV size and concentration. The red error bars of the histogram indicate +/- one standard error of the mean size. Examples are shown of different A1-EV sizes by negative stain in electron micrographs (insets). (b) Acetylcholineesterase activity was measured for cell culture media (control) or isolated normal human dermal fibroblast (NHDF) EVs, cardiosphere-derived cell (CDC)-EVs, and the newt A1-EVs by fluorescent enzyme assays. (c and d) Graphical representation of the amount of RNA per particle (femtograms) and protein per particle (picograms) for NHDF-, CDC- and A1-EVs following the 3-day serum-starvation protocol of cultured cells. (e-h) Western blots of common EV surface proteins from A1 cells and EVs and mammalian NHDF cells and EVs are shown. This set of markers is comprised of thrombospondin1 (TSP1), periostin (POSTN), fibronectin (FN) and CD9. Example FACS bead assay histogram data with A1-EVs and NHDF-EVs stained to detect TSP1 (i), POSTN (j), FN (k) and CD9 (l). Quantitative measurements for abundance levels expressed as % of control antibody stains are indicated in green text for each FACS assay.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/41598_2018_22450_Fig1_HTML-1-20190103084817.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Western Blot (WB)" title="CD9 Antibody (10626D) in WB"> <figcaption itemprop="caption">Figure 1 EVs were purified from hiPSCs and non-hiPSCs (Table S1 ) by Tim4-immobilized beads. Purified EVs (0.2 mug) were electrophoresed under non-reducing conditions on 5-20% polyacrylamide gels and visualized with sliver staining. Separated proteins were transferred to a PVDF membrane and blotted with anti-CD9 and anti-CD63.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_27423_ZJEV_A_1807674_F0004_OC.jpg?time=20240507?time=20240507" alt="CD9 Antibody in Dot Blot (DB)" title="CD9 Antibody (10626D) in DB"> <figcaption itemprop="caption">Figure 4. Analytical Size Exclusion Chromatography of hMSC-CM. (a) EV content of fractions evaluated by particle concentration measured by NTA i) (n = 3 x 60s videos, N = 3) and dot blots against CD63, CD81 and CD9 (N = 3, quantified by Normalised Relative Intensity, N.R.I, as in methods). (a) ii) Representative dot blot images as used for quantification in a) i) (images re-cut to align with graph). (b) Total protein and VEGF concentrations of each fraction (VEGF measured on pooled adjacent pairs and plotted at mid-point). (c) i) Quantification of total tubule length in tubule formation assay for pooled adjacent fraction pairs (N = 3, n = 6, mean +- SD of n shown for each N (replicates 1-3, R1-3)). (c) ii) Representative whole-well images of tubule structures formed in C i). Images inverted for clarity. Scale bar = 1 mm. (d) i) Quantification of wound closure rate for pooled adjacent fraction pairs (N = 3, n = 3, mean +- SD shown for each N (replicates 1-3, R1-3)). (d) ii) Representative images of scratch wounds for each fraction pair are shown for t = 15 h (endpoint). Scale bar = 100 mum. * and brackets: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001, (one-way within-subjects ANOVA and post-hoc Dunnett's test vs earliest measured fraction/s or negative control (NTA: F6, DB: F1, Total protein: F1, VEGF: F7-8, Tubule length: C-, Wound Closure Rate: C-)).</figcaption> </figure> </div> </div> </noscript> <div id="tf-related-products-container-new"></div> <div class="product-details"> <div class="container"> <div class="row"> <div class="large-span-12 medium-span-12 small-span-12"> <h2 class="pdp-sub-heading product_details_heading">Product Details</h2></div> <div class="hidden-sku"> <h2>10626D</h2> </div> <div class="product-detail-section tested-app-section new-tbl-design product-detail-section table-spec"> <div class="product-container-head"> <div class="product-item"> Applications </div> <div class="product-item dilution-lbl-head "> Tested Dilution </div> <div class="product-item published-applications"> Publications </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Western Blot (WB)</h2></div> <div class="product-item "> <span class="dilution-lbl">1 µg/mL</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#WB-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 57 publications</span> <span class="mobile-view">57 publications</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Immunocytochemistry (ICC/IF)</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#ICCIF-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 1 publication</span> <span class="mobile-view">1 publication</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Flow Cytometry (Flow)</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#Flow-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 1 publication</span> <span class="mobile-view">1 publication</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>ELISA (ELISA)</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#ELISA-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">-</span> <span class="mobile-view">-</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Immunoprecipitation (IP)</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#IP-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">-</span> <span class="mobile-view">-</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Dot blot (DB)</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#DB-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 5 publications</span> <span class="mobile-view">5 publications</span></a> </div> </div> </div> <div class="product-detail-section tested-app-section new-tbl-design product-specific-section table-spec"> <div class="product-container-head"> <div class="product-item">Product Specifications</div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Species Reactivity</h2> </div> <div class="product-item left-flex-cls"> <span title="Homo sapiens">Human</span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Published species</h2> </div> <div class="product-item left-flex-cls"> <span>Human,</span> <span>Mouse</span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2> Host/Isotype</h2> </div> <div class="product-item left-flex-cls"> <span title="Mus musculus">Mouse / IgG1</span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Class</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Monoclonal </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Type</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Antibody </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Clone</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Ts9 </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Immunogen </h2> </div> <div class="product-item left-flex-cls">CD9 purified from HeLa cells <script type="text/javascript"> if (typeof window.$mangular === 'undefined' || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = '[{"targetFamily":"CD9","uniProtId":"P21926-1","ncbiNodeId":"9606","antigenRange":"1-228","antigenLength":"228","antigenImageFileName":"10626D_CD9_P21926-1_house_mouse.svg","antigenImageFileNamePDP":"10626D_CD9_P21926-1_house_mouse_PDP.jpeg","sortOrder":"1"}]'; $mangular.isB2BCMGT = '"false"'; $mangular.isEpitopesModalImageMultiSizeEnabled = 'true'; </script> <div class="antigen-link" ng-click="$ctrl.openModal()"> <a href="">View immunogen <span class="antigen-link-icon"> <?xml version="1.0" encoding="utf-8"?>  <svg version="1.1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px" viewBox="0 0 20 20" style="enable-background:new 0 0 20 20;" xml:space="preserve"> <style type="text/css"> .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;} </style> <g id="Layer_1"> <line class="st0" x1="1.2" y1="2.5" x2="17.6" y2="2.5"/> <path d="M16.4,2.5"/> <g> <path d="M15.4,13.5"/> <path d="M15,14.9l2.8-2.8L15,9.3c0,0,0,0.9,0,1.9h-13V13h13C15.1,14,15.1,14.9,15,14.9z"/> <path d="M4.9,19.3l-2.8-2.8l2.8-2.8c0,0,0,0.9,0,1.9h13v1.8h-13C4.9,18.4,4.9,19.3,4.9,19.3z"/> </g> <g> <path d="M15.4,3.4"/> <path d="M15,6l2.8-2.8L15,0.3c0,0,0,0.9,0,1.9h-13V4h13C15.1,5.1,15.1,5.9,15,6z"/> <path d="M4.9,10.4L2.1,7.6l2.8-2.8c0,0,0,0.9,0,1.9h13v1.8h-13C4.9,9.5,4.9,10.4,4.9,10.4z"/> </g> <g> <path d="M2.8,8.1C1.3,8.1,0,6.9,0,5.4s1.3-2.7,2.8-2.7v1C1.8,3.6,1,4.4,1,5.4s0.8,1.8,1.8,1.8V8.1z"/> </g> <g> <path d="M17.2,12.6c1.5,0,2.8-1.2,2.8-2.8s-1.3-2.8-2.8-2.8v1c1,0,1.8,0.8,1.8,1.8s-0.8,1.8-1.8,1.8V12.6z"/> </g> <g> <path d="M2.8,17.1c-1.5,0-2.8-1.2-2.8-2.8s1.3-2.8,2.8-2.8v1c-1,0-1.8,0.8-1.8,1.8s0.8,1.8,1.8,1.8V17.1z"/> </g> </g> <g id="Layer_1_copy"> <path class="st1" d="M17.4,12.5c1.4-0.1,2.6-1.3,2.6-2.7c0-1.3-0.9-2.4-2.1-2.7V6.7h-13c0-1.1,0-1.9,0-1.9L2.6,7.1 C1.7,7,1,6.3,1,5.4C1,4.6,1.5,4,2.1,3.7V4h13c0,1,0,1.9,0,1.9l2.8-2.8L15,0.3c0,0,0,0.9,0,1.9h-13v0.4C0.9,3,0,4.1,0,5.4 C0,6.8,1.1,8,2.6,8.1l2.3,2.3c0,0,0-0.9,0-1.9h13V8.2C18.5,8.4,19,9.1,19,9.8c0,0.9-0.7,1.7-1.7,1.7L15,9.3c0,0,0,0.9,0,1.9h-13 v0.4C0.9,12,0,13,0,14.3c0,1.5,1.2,2.6,2.6,2.7l2.3,2.3c0,0,0-0.9,0-1.9h13v-1.8h-13c0-1.1,0-1.9,0-1.9L2.6,16 C1.7,15.9,1,15.2,1,14.3c0-0.7,0.5-1.3,1.1-1.6V13h13c0,1,0,1.9,0,1.9L17.4,12.5z"/> </g> </svg> </span> </a> </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Conjugate</h2> </div> <div class="product-item left-flex-cls"> <a ng-if="$ctrl.checkConjugate('Unconjugated ') && ''.trim() != ''" href="" ng-attr-target="{{$ctrl.getRequestCustomLinkTarget()}}" class="conjugate-value">Unconjugated </a> <span ng-if="($ctrl.checkConjugate('Unconjugated ') && ''.trim() == '')" class="conjugate-value">Unconjugated </span> <span ng-if="!$ctrl.checkConjugate('Unconjugated ')" class="conjugate-value">Unconjugated </span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Form</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Liquid </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Concentration</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> 0.5 mg/mL </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Purification</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> purified </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Storage buffer</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> PBS, pH 7.4 </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Contains</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> 0.09% sodium azide </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Storage conditions</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Shipping conditions</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Ambient </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"><h2>RRID <span id="rrid-tooltip" class="rrid-tooltip-icon" ng-include="'/order/genome-database/webapp/assets/core/img/svg/mono_tooltip.svg'" data-toggle="tooltip" ng-click="$ctrl.showRridTooltip()" close-tooltip="$ctrl.closeRridTooltip()" title="Research Resource Identifiers (#RRID) are ID numbers assigned to help researchers cite key resources in the biomedical literature to improve transparency of research methods."> </span></h2> </div> <div class="product-item left-flex-cls">AB_2532982</div> </div> </div> <div class="product-specific-details"> <div class="product-specific-details-tbl product-specific-add-border" > <div class="product-info-para"> <h4 class="product-info-para-heading"> Product Specific Information </h4> <p>The Exosome - anti-CD9 antibody recognizes the CD9/p24 antigen (~24 kDa), a member of the tetraspanin family. The antibody has been verified for western blotting of cellular and exosomal CD9 antigen.</p> </div> <div class="product-info-para"> <h4 class="product-info-para-heading"> Target Information </h4> <p class="product-specific-target-info">CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. CD9 belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression plays a critical role in the suppression of cancer cell motility and metastasis. CD9 is also considered as metastasis suppressor in solid tumors.</p> </div> <p class="product-details-regulatoryStmt">For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.</p> </div> <div class="product-detail-panel-builder"> <tf-promo-ad ng-if="$ctrl.showPromoAd" show-promo-ad="$ctrl.showPromoAd"></tf-promo-ad> </div> </div> </div> </div> </div> <div id="rootPdpDocLoader"></div> <div id="tf-related-products-container" ng-show="emptyRelatedProductsWidget === false" > <related-products-container ng-if="showRelatedProductsWidget === true" base-url="'/api/magellan/related-products-ui/widget/v2/'" product-type="'antibody'" product-sub-type="'primary'" product-id="'10626D'" country-code="'sg'" iso-language="'en'" > </related-products-container> </div> <div class="tf-pdp-divider" ng-if="emptyRelatedProductsWidget === false" ></div> <div class="references reference-section-3" id="references-component-id"> <div id="references-section-id"> <reference-section ng-if="loadReferences" default-display-card-limit="3" reference-count="64" sub-type="'Antibody'" 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B"],"speciesMap":{"Human":5},"journalsMap":{"J Extracell Vesicles":1,"Int J Mol Sci":1,"Langmuir":1,"ACS Nano":1,"J Mater Chem B":1},"sortedMap":{}}]}'></reference-section> </div> <noscript> <div itemscope itemtype="https://schema.org/ScholarlyArticle"> <h2>Product References</h2> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/37064893"> <div itemprop="name">Distinct microRNA and protein profiles of extracellular vesicles secreted from myotubes from morbidly obese donors with type 2 diabetes in response to electrical pulse stimulation.</div> </a> <div itemprop="about">Frontiers in physiology</div> <div itemprop="author">Aas V,Øvstebø R,Brusletto BS,Aspelin T,Trøseid AS,Qureshi S,Eid DSO,Olstad OK,Nyman TA,Haug KBF</div> <div itemprop="description">10626D was used in Western Blotting to examine how electrical pulse stimulation (EPS) of myotubes, a model of exercise, affects the cargo of released EVs.</div> <div itemprodp="datePublished">Tue Apr 18 00:00:00 EDT 2023</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/37064893"> <div itemprop="name">Distinct microRNA and protein profiles of extracellular vesicles secreted from myotubes from morbidly obese donors with type 2 diabetes in response to electrical pulse stimulation.</div> </a> <div itemprop="about">Frontiers in physiology</div> <div itemprop="author">Aas V,Øvstebø R,Brusletto BS,Aspelin T,Trøseid AS,Qureshi S,Eid DSO,Olstad OK,Nyman TA,Haug KBF</div> <div itemprop="description">10626D was used in Western Blotting to examine how electrical pulse stimulation (EPS) of myotubes, a model of exercise, affects the cargo of released EVs.</div> <div itemprodp="datePublished">Tue Apr 18 00:00:00 EDT 2023</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36969201"> <div itemprop="name">Release of exosomes in polytraumatized patients: The injury pattern is reflected by the surface epitopes.</div> </a> <div itemprop="about">Frontiers in immunology</div> <div itemprop="author">Weber B,Henrich D,Schindler CR,Marzi I,Leppik L</div> <div itemprop="description">10626D was used in Western Blot to show that the polytrauma injury pattern might be reflected by the cellular origin/surface epitopes of plasma-released exosomes immediately after trauma.</div> <div itemprodp="datePublished">Tue Mar 28 00:00:00 EDT 2023</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/37239852"> <div itemprop="name">Glycan Node Analysis Detects Varying Glycosaminoglycan Levels in Melanoma-Derived Extracellular Vesicles.</div> </a> <div itemprop="about">International journal of molecular sciences</div> <div itemprop="author">Pendiuk Goncalves J,Walker SA,Aguilar Díaz de León JS,Yang Y,Davidovich I,Busatto S,Sarkaria J,Talmon Y,Borges CR,Wolfram J</div> <div itemprop="description">10626D was used in Western Blotting to indicate that GNA can identify EV-associated glyco-polymers that would remain undetected with conventional MS methods.</div> <div itemprodp="datePublished">Tue May 09 00:00:00 EDT 2023</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/37239852"> <div itemprop="name">Glycan Node Analysis Detects Varying Glycosaminoglycan Levels in Melanoma-Derived Extracellular Vesicles.</div> </a> <div itemprop="about">International journal of molecular sciences</div> <div itemprop="author">Pendiuk Goncalves J,Walker SA,Aguilar Díaz de León JS,Yang Y,Davidovich I,Busatto S,Sarkaria J,Talmon Y,Borges CR,Wolfram J</div> <div itemprop="description">10626D was used in Western Blotting to indicate that GNA can identify EV-associated glyco-polymers that would remain undetected with conventional MS methods.</div> <div itemprodp="datePublished">Tue May 09 00:00:00 EDT 2023</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/37226100"> <div itemprop="name">Plasma-derived small extracellular vesicles unleash the angiogenic potential in head and neck cancer patients.</div> </a> <div itemprop="about">Molecular medicine (Cambridge, Mass.)</div> <div itemprop="author">Tengler L,Schütz J,Tiedtke M,Jablonska J,Theodoraki MN,Nitschke K,Weiß C,Seiz E,Affolter A,Jungbauer F,Lammert A,Rotter N,Ludwig S</div> <div itemprop="description">10626D was used in Western Blotting to isolate and characterize plasma small extracellular vesicles (sEVs) from head and neck cancer (HNC) patients, revealing that these sEVs predominantly carried anti-angiogenic proteins and suppressed angiogenic properties of endothelial cells (ECs), while sEVs from advanced-stage HNC patients exhibited pro-angiogenic effects compared to healthy donor (HD) sEVs, suggesting a potential role for tumor-derived sEVs in promoting angiogenesis in HNC.</div> <div itemprodp="datePublished">Wed May 24 00:00:00 EDT 2023</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/37226100"> <div itemprop="name">Plasma-derived small extracellular vesicles unleash the angiogenic potential in head and neck cancer patients.</div> </a> <div itemprop="about">Molecular medicine (Cambridge, Mass.)</div> <div itemprop="author">Tengler L,Schütz J,Tiedtke M,Jablonska J,Theodoraki MN,Nitschke K,Weiß C,Seiz E,Affolter A,Jungbauer F,Lammert A,Rotter N,Ludwig S</div> <div itemprop="description">10626D was used in Western Blotting to isolate and characterize plasma small extracellular vesicles (sEVs) from head and neck cancer (HNC) patients, revealing that these sEVs predominantly carried anti-angiogenic proteins and suppressed angiogenic properties of endothelial cells (ECs), while sEVs from advanced-stage HNC patients exhibited pro-angiogenic effects compared to healthy donor (HD) sEVs, suggesting a potential role for tumor-derived sEVs in promoting angiogenesis in HNC.</div> <div itemprodp="datePublished">Wed May 24 00:00:00 EDT 2023</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/37503786"> <div itemprop="name">Modulation of PD‑L1 expression by standard therapy in head and neck cancer cell lines and exosomes.</div> </a> <div itemprop="about">International journal of oncology</div> <div itemprop="author">Affolter A,Liebel K,Tengler L,Seiz E,Tiedtke M,Azhakesan A,Schütz J,Theodoraki MN,Kern J,Ruder AM,Fleckenstein J,Weis CA,Bieback K,Kramer B,Lammert A,Scherl C,Rotter N,Ludwig S</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</div> <div itemprodp="datePublished">Fri Sep 01 00:00:00 EDT 2023</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36986815"> <div itemprop="name">Clinically Expired Platelet Concentrates as a Source of Extracellular Vesicles for Targeted Anti-Cancer Drug Delivery.</div> </a> <div itemprop="about">Pharmaceutics</div> <div itemprop="author">Meliciano A,Salvador D,Mendonça P,Louro AF,Serra M</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</div> <div itemprodp="datePublished">Wed Mar 15 00:00:00 EDT 2023</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/37814342"> <div itemprop="name">Analysis of distribution, collection, and confirmation of capacity dependency of small extracellular vesicles toward a therapy for liver cirrhosis.</div> </a> <div itemprop="about">Inflammation and regeneration</div> <div itemprop="author">Takeda N,Tsuchiya A,Mito M,Natsui K,Natusi Y,Koseki Y,Tomiyoshi K,Yamazaki F,Yoshida Y,Abe H,Sano M,Kido T,Yoshioka Y,Kikuta J,Itoh T,Nishimura K,Ishii M,Ochiya T,Miyajima A,Terai S</div> <div itemprop="description">10626D was used in Western Blot to provide important fundamentals for the development of therapies using sEVs and hold potential implications for the therapeutic applications of sEV-based therapies for liver cirrhosis.</div> <div itemprodp="datePublished">Mon Oct 09 00:00:00 EDT 2023</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/37686214"> <div itemprop="name">Non-Coding RNA in Salivary Extracellular Vesicles: A New Frontier in Sjögren's Syndrome Diagnostics?</div> </a> <div itemprop="about">International journal of molecular sciences</div> <div itemprop="author">Cross T,Haug KBF,Brusletto BS,Ommundsen SK,Trøseid AS,Aspelin T,Olstad OK,Aass HCD,Galtung HK,Utheim TP,Jensen JL,Øvstebø R</div> <div itemprop="description">10626D was used in Western Blotting to demonstrate the potential for using salivary EV-RNA in primary Sjögren's syndrome diagnostics.</div> <div itemprodp="datePublished">Tue Aug 29 00:00:00 EDT 2023</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/37047109"> <div itemprop="name">Effect of Hypertrophic Scar Fibroblast-Derived Exosomes on Keratinocytes of Normal Human Skin.</div> </a> <div itemprop="about">International journal of molecular sciences</div> <div itemprop="author">Cui HS,Joo SY,Lee SY,Cho YS,Kim DH,Seo CH</div> <div itemprop="description">10626D was used in Western Blotting to indicate that HTSF-derived exosomes may play a role in the epidermal pathological development of hypertrophic scar.</div> <div itemprodp="datePublished">Fri Mar 24 00:00:00 EDT 2023</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34891059"> <div itemprop="name">The activity of alkaline phosphatase in breast cancer exosomes simplifies the biosensing design.</div> </a> <div itemprop="about">Biosensors & bioelectronics</div> <div itemprop="author">Moura SL,Pallarès-Rusiñol A,Sappia L,Martí M,Pividori MI</div> <div itemprop="description">10626D was used in Flow cytometry/Cell sorting to addresses a biosensor combining the immunomagnetic separation and the electrochemical biosensing based on the intrinsic ALP activity of the exosomes.</div> <div itemprodp="datePublished">Tue Feb 15 00:00:00 EST 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/35513867"> <div itemprop="name">Oligodendroglia-derived extracellular vesicles activate autophagy via LC3B/BAG3 to protect against oxidative stress with an enhanced effect for HSPB8 enriched vesicles.</div> </a> <div itemprop="about">Cell communication and signaling : CCS</div> <div itemprop="author">Van den Broek B,Wuyts C,Sisto A,Pintelon I,Timmermans JP,Somers V,Timmerman V,Hellings N,Irobi J</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</div> <div itemprodp="datePublished">Thu May 05 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/35367893"> <div itemprop="name">Investigating heparin affinity chromatography for extracellular vesicle purification and fractionation.</div> </a> <div itemprop="about">Journal of chromatography. A</div> <div itemprop="author">Barnes B,Caws T,Thomas S,Shephard AP,Corteling R,Hole P,Bracewell DG</div> <div itemprop="description">10626D was used in Western Blotting to find that heparin affinity chromatography could remove on average 98.8% and 99.0% of residual protein and DNA respectively.</div> <div itemprodp="datePublished">Tue May 10 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/35777918"> <div itemprop="name">Demystifying the long noncoding RNA landscape of small EVs derived from human mesenchymal stromal cells.</div> </a> <div itemprop="about">Journal of advanced research</div> <div itemprop="author">Lee CW,Chen YF,Hsiao AW,Wang AY,Shen OY,Wang BY,Ho LWC,Lin WT,Choi CHJ,Lee OK</div> <div itemprop="description">10626D was used in Western Blotting to find that the proteins interacting with medicinal signaling lncRNAs or licensing-responsive lncRNAs have a tight interaction network involved in chromatin remodeling, SWI/SNF superfamily type complexes, and histone binding.</div> <div itemprodp="datePublished">Fri Jul 01 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36181394"> <div itemprop="name">Usage of celery root exosome as an immune suppressant; Lipidomic characterization of apium graveolens originated exosomes and its suppressive effect on PMA/ionomycin mediated CD4<sup>+</sup> T lymphocyte activation.</div> </a> <div itemprop="about">Journal of food biochemistry</div> <div itemprop="author">Taşlı PN</div> <div itemprop="description">10626D was used in Western Blotting to suppress the inflammatory response of T lymphocytes using exosomes isolated from a known anti-inflammatory plant.</div> <div itemprodp="datePublished">Thu Dec 01 00:00:00 EST 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/35997754"> <div itemprop="name">Late Breaking Orals.</div> </a> <div itemprop="about">Journal of extracellular vesicles</div> <div itemprop="author">No authors listed</div> <div itemprop="description">10626D was used in Western Blotting to investagate a hypothesis that extracellular vesicles (EVs) released from Leishmania-infected cells (LiEVs) that contain parasite derived molecules, induce cellular responses that promote tissue remodeling.</div> <div itemprodp="datePublished">Mon Aug 01 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/35887064"> <div itemprop="name">Effect of Pre-Processing Storage Condition of Cell Culture-Conditioned Medium on Extracellular Vesicles Derived from Human Umbilical Cord-Derived Mesenchymal Stromal Cells.</div> </a> <div itemprop="about">International journal of molecular sciences</div> <div itemprop="author">Wright A,Snyder OL,Christenson LK,He H,Weiss ML</div> <div itemprop="description">10626D was used in Western Blot, Dot Blot to suggest that the CM produced during GMP-manufacturing of MSCs for clinical applications might be stored at -80 °C prior to EV isolation, and this may enable production scale-up, and thus, and enable preclinical and clinical testing, and EV lot qualification.</div> <div itemprodp="datePublished">Wed Jul 13 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/35787656"> <div itemprop="name">Nano pom-poms prepared exosomes enable highly specific cancer biomarker detection.</div> </a> <div itemprop="about">Communications biology</div> <div itemprop="author">He N,Thippabhotla S,Zhong C,Greenberg Z,Xu L,Pessetto Z,Godwin AK,Zeng Y,He M</div> <div itemprop="description">10626D was used in Western Blotting to introduce a novel 3D-structured nanographene immunomagnetic particles (NanoPoms) with unique flower pom-poms morphology and photo-click chemistry for specific marker-defined capture and release of intact exosome.</div> <div itemprodp="datePublished">Mon Jul 04 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36293369"> <div itemprop="name">Characteristics of Exosomes and the Vascular Landscape Regulate Exosome Sequestration by Peripheral Tissues and Brain.</div> </a> <div itemprop="about">International journal of molecular sciences</div> <div itemprop="author">Banks WA,Sharma P,Hansen KM,Ludwig N,Whiteside TL</div> <div itemprop="description">10626D was used in Western Blotting to explore whether exosome tissue sequestration is determined by the exosomes or the tissues using ten radiolabeled exosomes from human or murine, cancerous or noncancerous cell lines.</div> <div itemprodp="datePublished">Wed Oct 19 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34735133"> <div itemprop="name">Single Particle Automated Raman Trapping Analysis of Breast Cancer Cell-Derived Extracellular Vesicles as Cancer Biomarkers.</div> </a> <div itemprop="about">ACS nano</div> <div itemprop="author">Penders J,Nagelkerke A,Cunnane EM,Pedersen SV,Pence IJ,Coombes RC,Stevens MM</div> <div itemprop="description">10626D was used in Dot blot, Western Blot to show that the predictive ability of our approach is consistent across multiple EV isolations from the same cell types.</div> <div itemprodp="datePublished">Tue Nov 23 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34735133"> <div itemprop="name">Single Particle Automated Raman Trapping Analysis of Breast Cancer Cell-Derived Extracellular Vesicles as Cancer Biomarkers.</div> </a> <div itemprop="about">ACS nano</div> <div itemprop="author">Penders J,Nagelkerke A,Cunnane EM,Pedersen SV,Pence IJ,Coombes RC,Stevens MM</div> <div itemprop="description">10626D was used in Dot blot, Western Blot to show that the predictive ability of our approach is consistent across multiple EV isolations from the same cell types.</div> <div itemprodp="datePublished">Tue Nov 23 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33448084"> <div itemprop="name">Unsupervised Machine Learning-Based Clustering of Nanosized Fluorescent Extracellular Vesicles.</div> </a> <div itemprop="about">Small (Weinheim an der Bergstrasse, Germany)</div> <div itemprop="author">Kuypers S,Smisdom N,Pintelon I,Timmermans JP,Ameloot M,Michiels L,Hendrix J,Hosseinkhani B</div> <div itemprop="description">10626D was used in Western Blot to assess both the purity and the inflammatory status of EV, and compare cell culture and plasma-derived EV isolated via different purification methods.</div> <div itemprodp="datePublished">Mon Feb 01 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32372373"> <div itemprop="name">The metalloproteinase ADAM10 requires its activity to sustain surface expression.</div> </a> <div itemprop="about">Cellular and molecular life sciences : CMLS</div> <div itemprop="author">Seifert A,Düsterhöft S,Wozniak J,Koo CZ,Tomlinson MG,Nuti E,Rossello A,Cuffaro D,Yildiz D,Ludwig A</div> <div itemprop="description">10626D was used in Western Blot to demonstrate that loss of proteolytic activity of ADAM10 by either inhibition or loss of function mutations induces removal of the protease from the cell surface and the whole cell.</div> <div itemprodp="datePublished">Fri Jan 01 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32372373"> <div itemprop="name">The metalloproteinase ADAM10 requires its activity to sustain surface expression.</div> </a> <div itemprop="about">Cellular and molecular life sciences : CMLS</div> <div itemprop="author">Seifert A,Düsterhöft S,Wozniak J,Koo CZ,Tomlinson MG,Nuti E,Rossello A,Cuffaro D,Yildiz D,Ludwig A</div> <div itemprop="description">10626D was used in Western Blot to demonstrate that loss of proteolytic activity of ADAM10 by either inhibition or loss of function mutations induces removal of the protease from the cell surface and the whole cell.</div> <div itemprodp="datePublished">Fri Jan 01 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34558650"> <div itemprop="name">Exosomes in serum‑free cultures of THP‑1 macrophages infected with <i>Mycobacterium tuberculosis</i>.</div> </a> <div itemprop="about">Molecular medicine reports</div> <div itemprop="author">Biadglegne F,Rademacher P,De Sulbaran YGJ,König B,Rodloff AC,Zedler U,Dorhoi A,Sack U</div> <div itemprop="description">10626D was used in Western Blotting to characterize exosomes released from THP‚1 macrophages cultured in serum‚free, ultra‚centrifuged medium upon infection with the human pathogen Mycobacterium tuberculosis (Mtb).</div> <div itemprodp="datePublished">Mon Nov 01 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34558650"> <div itemprop="name">Exosomes in serum‑free cultures of THP‑1 macrophages infected with <i>Mycobacterium tuberculosis</i>.</div> </a> <div itemprop="about">Molecular medicine reports</div> <div itemprop="author">Biadglegne F,Rademacher P,De Sulbaran YGJ,König B,Rodloff AC,Zedler U,Dorhoi A,Sack U</div> <div itemprop="description">10626D was used in Western Blotting to characterize exosomes released from THP‚1 macrophages cultured in serum‚free, ultra‚centrifuged medium upon infection with the human pathogen Mycobacterium tuberculosis (Mtb).</div> <div itemprodp="datePublished">Mon Nov 01 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33926561"> <div itemprop="name">Curcumin-primed human BMSC-derived extracellular vesicles reverse IL-1β-induced catabolic responses of OA chondrocytes by upregulating miR-126-3p.</div> </a> <div itemprop="about">Stem cell research & therapy</div> <div itemprop="author">Li S,Stöckl S,Lukas C,Herrmann M,Brochhausen C,König MA,Johnstone B,Grässel S</div> <div itemprop="description">10626D was used in Western Blotting to evaluate modulatory effects of curcumin-primed human (h)BMSC-derived EVs (Cur-EVs) on IL-1β stimulated human osteoarthritic chondrocytes (OA-CH).</div> <div itemprodp="datePublished">Thu Apr 29 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33926561"> <div itemprop="name">Curcumin-primed human BMSC-derived extracellular vesicles reverse IL-1β-induced catabolic responses of OA chondrocytes by upregulating miR-126-3p.</div> </a> <div itemprop="about">Stem cell research & therapy</div> <div itemprop="author">Li S,Stöckl S,Lukas C,Herrmann M,Brochhausen C,König MA,Johnstone B,Grässel S</div> <div itemprop="description">10626D was used in Western Blotting to evaluate modulatory effects of curcumin-primed human (h)BMSC-derived EVs (Cur-EVs) on IL-1β stimulated human osteoarthritic chondrocytes (OA-CH).</div> <div itemprodp="datePublished">Thu Apr 29 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34439329"> <div itemprop="name">Small Extracellular Vesicles from Head and Neck Squamous Cell Carcinoma Cells Carry a Proteomic Signature for Tumor Hypoxia.</div> </a> <div itemprop="about">Cancers</div> <div itemprop="author">Głuszko A,Szczepański MJ,Whiteside TL,Reichert TE,Siewiera J,Ludwig N</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</div> <div itemprodp="datePublished">Thu Aug 19 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33924377"> <div itemprop="name">A Simple and Quick Method for Loading Proteins in Extracellular Vesicles.</div> </a> <div itemprop="about">Pharmaceuticals (Basel, Switzerland)</div> <div itemprop="author">Busatto S,Iannotta D,Walker SA,Di Marzio L,Wolfram J</div> <div itemprop="description">10626D was used in Western Blotting to indicate that EVs retain native features following protein-loading and obtain similar levels of intracellular protein delivery as conventional methods, but display less toxicity.</div> <div itemprodp="datePublished">Tue Apr 13 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33924377"> <div itemprop="name">A Simple and Quick Method for Loading Proteins in Extracellular Vesicles.</div> </a> <div itemprop="about">Pharmaceuticals (Basel, Switzerland)</div> <div itemprop="author">Busatto S,Iannotta D,Walker SA,Di Marzio L,Wolfram J</div> <div itemprop="description">10626D was used in Western Blotting to indicate that EVs retain native features following protein-loading and obtain similar levels of intracellular protein delivery as conventional methods, but display less toxicity.</div> <div itemprodp="datePublished">Tue Apr 13 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34716331"> <div itemprop="name">The membrane associated accessory protein is an adeno-associated viral egress factor.</div> </a> <div itemprop="about">Nature communications</div> <div itemprop="author">Elmore ZC,Patrick Havlik L,Oh DK,Anderson L,Daaboul G,Devlin GW,Vincent HA,Asokan A</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</div> <div itemprodp="datePublished">Fri Oct 29 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34754007"> <div itemprop="name">Plasma extracellular vesicles in people living with HIV and type 2 diabetes are related to microbial translocation and cardiovascular risk.</div> </a> <div itemprop="about">Scientific reports</div> <div itemprop="author">Vestad B,Nyman TA,Hove-Skovsgaard M,Stensland M,Hoel H,Trøseid AS,Aspelin T,Aass HCD,Puhka M,Hov JR,Nielsen SD,Øvstebø R,Trøseid M</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</div> <div itemprodp="datePublished">Tue Nov 09 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34754007"> <div itemprop="name">Plasma extracellular vesicles in people living with HIV and type 2 diabetes are related to microbial translocation and cardiovascular risk.</div> </a> <div itemprop="about">Scientific reports</div> <div itemprop="author">Vestad B,Nyman TA,Hove-Skovsgaard M,Stensland M,Hoel H,Trøseid AS,Aspelin T,Aass HCD,Puhka M,Hov JR,Nielsen SD,Øvstebø R,Trøseid M</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</div> <div itemprodp="datePublished">Tue Nov 09 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34404763"> <div itemprop="name">Lysosome purinergic receptor P2X4 regulates neoangiogenesis induced by microvesicles from sarcoma patients.</div> </a> <div itemprop="about">Cell death & disease</div> <div itemprop="author">Palinski W,Monti M,Camerlingo R,Iacobucci I,Bocella S,Pinto F,Iannuzzi C,Mansueto G,Pignatiello S,Fazioli F,Gallo M,Marra L,Cozzolino F,De Chiara A,Pucci P,Bilancio A,de Nigris F</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</div> <div itemprodp="datePublished">Tue Aug 17 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34277703"> <div itemprop="name">Serum Extracellular Vesicle-Derived miR-124-3p as a Diagnostic and Predictive Marker for Early-Stage Acute Ischemic Stroke.</div> </a> <div itemprop="about">Frontiers in molecular biosciences</div> <div itemprop="author">Qi Z,Zhao Y,Su Y,Cao B,Yang JJ,Xing Q</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</div> <div itemprodp="datePublished">Tue Jul 20 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34262466"> <div itemprop="name"><i>Tsc</i> Gene Locus Disruption and Differences in Renal Epithelial Extracellular Vesicles.</div> </a> <div itemprop="about">Frontiers in physiology</div> <div itemprop="author">Kumar P,Zadjali F,Yao Y,Siroky B,Astrinidis A,Gross KW,Bissler JJ</div> <div itemprop="description">10626D was used in Western Blot to report a comparative analysis of EVs derived from isogenic renal cells except for Tsc1 or Tsc2 gene status and hypothesized that in spite of having similar physical characteristics, EVs modulate signaling pathways differently, thus leading to TSC heterogenicity.</div> <div itemprodp="datePublished">Fri Jul 16 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34262466"> <div itemprop="name"><i>Tsc</i> Gene Locus Disruption and Differences in Renal Epithelial Extracellular Vesicles.</div> </a> <div itemprop="about">Frontiers in physiology</div> <div itemprop="author">Kumar P,Zadjali F,Yao Y,Siroky B,Astrinidis A,Gross KW,Bissler JJ</div> <div itemprop="description">10626D was used in Western Blot to report a comparative analysis of EVs derived from isogenic renal cells except for Tsc1 or Tsc2 gene status and hypothesized that in spite of having similar physical characteristics, EVs modulate signaling pathways differently, thus leading to TSC heterogenicity.</div> <div itemprodp="datePublished">Fri Jul 16 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34650557"> <div itemprop="name">Serum CD203c+ Extracellular Vesicle Serves as a Novel Diagnostic and Prognostic Biomarker for Succinylated Gelatin Induced Perioperative Hypersensitive Reaction.</div> </a> <div itemprop="about">Frontiers in immunology</div> <div itemprop="author">Qi Z,Xue Q,Wang H,Cao B,Su Y,Xing Q,Yang JJ</div> <div itemprop="description">10626D was used in Western Blot to study extracellular vesicles from patients with perioperative hypersensitivity reaction with western blot.</div> <div itemprodp="datePublished">Fri Dec 24 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34650557"> <div itemprop="name">Serum CD203c+ Extracellular Vesicle Serves as a Novel Diagnostic and Prognostic Biomarker for Succinylated Gelatin Induced Perioperative Hypersensitive Reaction.</div> </a> <div itemprop="about">Frontiers in immunology</div> <div itemprop="author">Qi Z,Xue Q,Wang H,Cao B,Su Y,Xing Q,Yang JJ</div> <div itemprop="description">10626D was used in Western Blot to study extracellular vesicles from patients with perioperative hypersensitivity reaction with western blot.</div> <div itemprodp="datePublished">Fri Dec 24 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33936570"> <div itemprop="name">Quantitative proteomic analysis of extracellular vesicle subgroups isolated by an optimized method combining polymer-based precipitation and size exclusion chromatography.</div> </a> <div itemprop="about">Journal of extracellular vesicles</div> <div itemprop="author">Martínez-Greene JA,Hernández-Ortega K,Quiroz-Baez R,Resendis-Antonio O,Pichardo-Casas I,Sinclair DA,Budnik B,Hidalgo-Miranda A,Uribe-Querol E,Ramos-Godínez MDP,Martínez-Martínez E</div> <div itemprop="description"></div> <div itemprodp="datePublished">Thu Apr 01 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33602965"> <div itemprop="name">Integrins mediate placental extracellular vesicle trafficking to lung and liver in vivo.</div> </a> <div itemprop="about">Scientific reports</div> <div itemprop="author">Nguyen SL,Ahn SH,Greenberg JW,Collaer BW,Agnew DW,Arora R,Petroff MG</div> <div itemprop="description">10626D was used in Western Blotting to study placental extracellular vesicles and their trafficking mechanisms.</div> <div itemprodp="datePublished">Thu Feb 18 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33704724"> <div itemprop="name">Immunoaffinity-Based Isolation of Melanoma Cell-Derived and T Cell-Derived Exosomes from Plasma of Melanoma Patients.</div> </a> <div itemprop="about">Methods in molecular biology (Clifton, N.J.)</div> <div itemprop="author">Mondal SK,Whiteside TL</div> <div itemprop="description">10626D was used in Western Blotting to show that the immune capture method enables the recovery of MTEX and CD3+ exosomes in quantities sufficient both for molecular profiling by flow cytometry or western blotting and for functional analyses.</div> <div itemprodp="datePublished">Thu Apr 01 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32373878"> <div itemprop="name">Molecular imaging of extracellular vesicles in vitro via Raman metabolic labelling.</div> </a> <div itemprop="about">Journal of materials chemistry. B</div> <div itemprop="author">Horgan CC,Nagelkerke A,Whittaker TE,Nele V,Massi L,Kauscher U,Penders J,Bergholt MS,Hood SR,Stevens MM</div> <div itemprop="description">10626D was used in Western Blot, Dot blot to enable a deeper understanding of many of the biological mechanisms underpinning EVs, with profound implications for the design of EVs as therapeutic delivery vectors and applications as disease biomarkers.</div> <div itemprodp="datePublished">Wed May 27 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32373878"> <div itemprop="name">Molecular imaging of extracellular vesicles in vitro via Raman metabolic labelling.</div> </a> <div itemprop="about">Journal of materials chemistry. B</div> <div itemprop="author">Horgan CC,Nagelkerke A,Whittaker TE,Nele V,Massi L,Kauscher U,Penders J,Bergholt MS,Hood SR,Stevens MM</div> <div itemprop="description">10626D was used in Western Blot, Dot blot to enable a deeper understanding of many of the biological mechanisms underpinning EVs, with profound implications for the design of EVs as therapeutic delivery vectors and applications as disease biomarkers.</div> <div itemprodp="datePublished">Wed May 27 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32051269"> <div itemprop="name">Human Immunodeficiency Virus-Associated Exosomes Promote Kaposi's Sarcoma-Associated Herpesvirus Infection via the Epidermal Growth Factor Receptor.</div> </a> <div itemprop="about">Journal of virology</div> <div itemprop="author">Chen L,Feng Z,Yuan G,Emerson CC,Stewart PL,Ye F,Jin G</div> <div itemprop="description">10626D was used in Western Blotting to reveal that the transmission Kaposi''s sarcoma-associated herpesvirus (KSHV) infection, which is promoted by the exosomes in the saliva of people living with human immunodeficiency virus (HIV), can reduced by the inhibition of epidermal growth factor receptor (EGFR).</div> <div itemprodp="datePublished">Thu Apr 16 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33425869"> <div itemprop="name">hBMSC-Derived Extracellular Vesicles Attenuate IL-1β-Induced Catabolic Effects on OA-Chondrocytes by Regulating Pro-inflammatory Signaling Pathways.</div> </a> <div itemprop="about">Frontiers in bioengineering and biotechnology</div> <div itemprop="author">Li S,Stöckl S,Lukas C,Götz J,Herrmann M,Federlin M,Grässel S</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</div> <div itemprodp="datePublished">Tue Jan 12 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33425869"> <div itemprop="name">hBMSC-Derived Extracellular Vesicles Attenuate IL-1β-Induced Catabolic Effects on OA-Chondrocytes by Regulating Pro-inflammatory Signaling Pathways.</div> </a> <div itemprop="about">Frontiers in bioengineering and biotechnology</div> <div itemprop="author">Li S,Stöckl S,Lukas C,Götz J,Herrmann M,Federlin M,Grässel S</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</div> <div itemprodp="datePublished">Tue Jan 12 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33425878"> <div itemprop="name">Influence of Extracellular Vesicles Isolated From Osteoblasts of Patients With Cox-Arthrosis and/or Osteoporosis on Metabolism and Osteogenic Differentiation of BMSCs.</div> </a> <div itemprop="about">Frontiers in bioengineering and biotechnology</div> <div itemprop="author">Niedermair T,Lukas C,Li S,Stöckl S,Craiovan B,Brochhausen C,Federlin M,Herrmann M,Grässel S</div> <div itemprop="description">10626D was used in Western Blotting to show that EVs isolated from osteoblasts of patients with hip OA (coxarthrosis/CA), osteoporosis (OP), or a combination of both (CA/OP) might negatively affect metabolism and osteogenic differentiation of bone-marrow derived (B)MSCs.</div> <div itemprodp="datePublished">Tue Jan 12 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33425878"> <div itemprop="name">Influence of Extracellular Vesicles Isolated From Osteoblasts of Patients With Cox-Arthrosis and/or Osteoporosis on Metabolism and Osteogenic Differentiation of BMSCs.</div> </a> <div itemprop="about">Frontiers in bioengineering and biotechnology</div> <div itemprop="author">Niedermair T,Lukas C,Li S,Stöckl S,Craiovan B,Brochhausen C,Federlin M,Herrmann M,Grässel S</div> <div itemprop="description">10626D was used in Western Blotting to show that EVs isolated from osteoblasts of patients with hip OA (coxarthrosis/CA), osteoporosis (OP), or a combination of both (CA/OP) might negatively affect metabolism and osteogenic differentiation of bone-marrow derived (B)MSCs.</div> <div itemprodp="datePublished">Tue Jan 12 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33125101"> <div itemprop="name">Oral cancer cell‑derived exosomes modulate natural killer cell activity by regulating the receptors on these cells.</div> </a> <div itemprop="about">International journal of molecular medicine</div> <div itemprop="author">Zhu X,Qin X,Wang X,Wang Y,Cao W,Zhang J,Chen W</div> <div itemprop="description">10626D was used in Western Blotting to reveal changes in NK cell function and provide new insight into NK cell dysfunction.</div> <div itemprodp="datePublished">Tue Dec 01 00:00:00 EST 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33125101"> <div itemprop="name">Oral cancer cell‑derived exosomes modulate natural killer cell activity by regulating the receptors on these cells.</div> </a> <div itemprop="about">International journal of molecular medicine</div> <div itemprop="author">Zhu X,Qin X,Wang X,Wang Y,Cao W,Zhang J,Chen W</div> <div itemprop="description">10626D was used in Western Blotting to reveal changes in NK cell function and provide new insight into NK cell dysfunction.</div> <div itemprodp="datePublished">Tue Dec 01 00:00:00 EST 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32250120"> <div itemprop="name">Gold Nanocluster Extracellular Vesicle Supraparticles: Self-Assembled Nanostructures for Three-Dimensional Uptake Visualization.</div> </a> <div itemprop="about">Langmuir : the ACS journal of surfaces and colloids</div> <div itemprop="author">Kauscher U,Penders J,Nagelkerke A,Holme MN,Nele V,Massi L,Gopal S,Whittaker TE,Stevens MM</div> <div itemprop="description">10626D was used in Dot blot to show that extracellular vesicles combined with hydrophobic gold nanoclusters (AuNCs) can self-assemble into supraparticles.</div> <div itemprodp="datePublished">Tue Apr 14 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/31963351"> <div itemprop="name">Identification of miRNAs Enriched in Extracellular Vesicles Derived from Serum Samples of Breast Cancer Patients.</div> </a> <div itemprop="about">Biomolecules</div> <div itemprop="author">Ozawa PMM,Vieira E,Lemos DS,Souza ILM,Zanata SM,Pankievicz VC,Tuleski TR,Souza EM,Wowk PF,Urban CA,Kuroda F,Lima RS,Almeida RC,Gradia DF,Cavalli IJ,Cavalli LR,Malheiros D,Ribeiro EMSF</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</div> <div itemprodp="datePublished">Thu Jan 16 00:00:00 EST 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32466374"> <div itemprop="name">The Potential of CD16 on Plasma-Derived Exosomes as a Liquid Biomarker in Head and Neck Cancer.</div> </a> <div itemprop="about">International journal of molecular sciences</div> <div itemprop="author">Hofmann L,Ludwig S,Schuler PJ,Hoffmann TK,Brunner C,Theodoraki MN</div> <div itemprop="description">10626D was used in Western Blotting to demonstrate that exosomes have the potential to serve as easily accessible, non-invasive biomarkers for tumor status and tumor aggressiveness as well as for the degree of immune suppression in HNSCC patients.</div> <div itemprodp="datePublished">Tue May 26 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32466374"> <div itemprop="name">The Potential of CD16 on Plasma-Derived Exosomes as a Liquid Biomarker in Head and Neck Cancer.</div> </a> <div itemprop="about">International journal of molecular sciences</div> <div itemprop="author">Hofmann L,Ludwig S,Schuler PJ,Hoffmann TK,Brunner C,Theodoraki MN</div> <div itemprop="description">10626D was used in Western Blotting to demonstrate that exosomes have the potential to serve as easily accessible, non-invasive biomarkers for tumor status and tumor aggressiveness as well as for the degree of immune suppression in HNSCC patients.</div> <div itemprodp="datePublished">Tue May 26 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32842648"> <div itemprop="name">Glycan Node Analysis of Plasma-Derived Extracellular Vesicles.</div> </a> <div itemprop="about">Cells</div> <div itemprop="author">Walker SA,Aguilar Díaz De León JS,Busatto S,Wurtz GA,Zubair AC,Borges CR,Wolfram J</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</div> <div itemprodp="datePublished">Sat Aug 22 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32944192"> <div itemprop="name">Experimental artefacts can lead to misattribution of bioactivity from soluble mesenchymal stem cell paracrine factors to extracellular vesicles.</div> </a> <div itemprop="about">Journal of extracellular vesicles</div> <div itemprop="author">Whittaker TE,Nagelkerke A,Nele V,Kauscher U,Stevens MM</div> <div itemprop="description">10626D was used in Dot blot to investigate the relative contribution of extracellular vesicles in in vitro models for angiogenesis and wound healing.</div> <div itemprodp="datePublished">Wed Aug 26 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32944192"> <div itemprop="name">Experimental artefacts can lead to misattribution of bioactivity from soluble mesenchymal stem cell paracrine factors to extracellular vesicles.</div> </a> <div itemprop="about">Journal of extracellular vesicles</div> <div itemprop="author">Whittaker TE,Nagelkerke A,Nele V,Kauscher U,Stevens MM</div> <div itemprop="description">10626D was used in Dot blot to investigate the relative contribution of extracellular vesicles in in vitro models for angiogenesis and wound healing.</div> <div itemprodp="datePublished">Wed Aug 26 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32054015"> <div itemprop="name">Matrix Effect in the Isolation of Breast Cancer-Derived Nanovesicles by Immunomagnetic Separation and Electrochemical Immunosensing-A Comparative Study.</div> </a> <div itemprop="about">Sensors (Basel, Switzerland)</div> <div itemprop="author">Lima Moura S,Martì M,Pividori MI</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Immunocytochemistry</div> <div itemprodp="datePublished">Tue Feb 11 00:00:00 EST 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32300120"> <div itemprop="name">Improving extracellular vesicles visualization: From static to motion.</div> </a> <div itemprop="about">Scientific reports</div> <div itemprop="author">Reclusa P,Verstraelen P,Taverna S,Gunasekaran M,Pucci M,Pintelon I,Claes N,de Miguel-Pérez D,Alessandro R,Bals S,Kaushal S,Rolfo C</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</div> <div itemprodp="datePublished">Thu Apr 16 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32300120"> <div itemprop="name">Improving extracellular vesicles visualization: From static to motion.</div> </a> <div itemprop="about">Scientific reports</div> <div itemprop="author">Reclusa P,Verstraelen P,Taverna S,Gunasekaran M,Pucci M,Pintelon I,Claes N,de Miguel-Pérez D,Alessandro R,Bals S,Kaushal S,Rolfo C</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</div> <div itemprodp="datePublished">Thu Apr 16 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32751214"> <div itemprop="name">Circulating Exosomes Inhibit B Cell Proliferation and Activity.</div> </a> <div itemprop="about">Cancers</div> <div itemprop="author">Schroeder JC,Puntigam L,Hofmann L,Jeske SS,Beccard IJ,Doescher J,Laban S,Hoffmann TK,Brunner C,Theodoraki MN,Schuler PJ</div> <div itemprop="description">10626D was used in Western Blotting to isolate and analyse peripheral B cells by flow cytometry to understand the effects of exosomes on B cells.</div> <div itemprodp="datePublished">Wed Jul 29 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32751214"> <div itemprop="name">Circulating Exosomes Inhibit B Cell Proliferation and Activity.</div> </a> <div itemprop="about">Cancers</div> <div itemprop="author">Schroeder JC,Puntigam L,Hofmann L,Jeske SS,Beccard IJ,Doescher J,Laban S,Hoffmann TK,Brunner C,Theodoraki MN,Schuler PJ</div> <div itemprop="description">10626D was used in Western Blotting to isolate and analyse peripheral B cells by flow cytometry to understand the effects of exosomes on B cells.</div> <div itemprodp="datePublished">Wed Jul 29 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32886718"> <div itemprop="name">Cancer cell line-specific protein profiles in extracellular vesicles identified by proteomics.</div> </a> <div itemprop="about">PloS one</div> <div itemprop="author">Guerreiro EM,Øvstebø R,Thiede B,Costea DE,Søland TM,Kanli Galtung H</div> <div itemprop="description">10626D was used in Western Blotting to characterize and compare the protein content of Extracellular vesicles (EVs) derived from three different cancer cell lines in search of a specific molecular signature, with emphasis on proteins related to the carcinogenic process.</div> <div itemprodp="datePublished">Thu Oct 29 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32886718"> <div itemprop="name">Cancer cell line-specific protein profiles in extracellular vesicles identified by proteomics.</div> </a> <div itemprop="about">PloS one</div> <div itemprop="author">Guerreiro EM,Øvstebø R,Thiede B,Costea DE,Søland TM,Kanli Galtung H</div> <div itemprop="description">10626D was used in Western Blotting to characterize and compare the protein content of Extracellular vesicles (EVs) derived from three different cancer cell lines in search of a specific molecular signature, with emphasis on proteins related to the carcinogenic process.</div> <div itemprodp="datePublished">Thu Oct 29 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32793278"> <div itemprop="name">Plasma Small Extracellular Vesicles Derived miR-21-5p and miR-92a-3p as Potential Biomarkers for Hepatocellular Carcinoma Screening.</div> </a> <div itemprop="about">Frontiers in genetics</div> <div itemprop="author">Sorop A,Iacob R,Iacob S,Constantinescu D,Chitoiu L,Fertig TE,Dinischiotu A,Chivu-Economescu M,Bacalbasa N,Savu L,Gheorghe L,Dima S,Popescu I</div> <div itemprop="description">10626D was used in Western Blotting to investigate the utility of plasma exosomal miR-21-5p and miR-92-3p for HCC diagnosis during screening protocols.</div> <div itemprodp="datePublished">Sat Apr 16 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32793278"> <div itemprop="name">Plasma Small Extracellular Vesicles Derived miR-21-5p and miR-92a-3p as Potential Biomarkers for Hepatocellular Carcinoma Screening.</div> </a> <div itemprop="about">Frontiers in genetics</div> <div itemprop="author">Sorop A,Iacob R,Iacob S,Constantinescu D,Chitoiu L,Fertig TE,Dinischiotu A,Chivu-Economescu M,Bacalbasa N,Savu L,Gheorghe L,Dima S,Popescu I</div> <div itemprop="description">10626D was used in Western Blotting to investigate the utility of plasma exosomal miR-21-5p and miR-92-3p for HCC diagnosis during screening protocols.</div> <div itemprodp="datePublished">Sat Apr 16 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32386350"> <div itemprop="name">Premature senescence in human melanocytes after exposure to solar UVR: An exosome and UV-miRNA connection.</div> </a> <div itemprop="about">Pigment cell & melanoma research</div> <div itemprop="author">Sha J,Arbesman J,Harter ML</div> <div itemprop="description">10626D was used in Western Blotting to study the effects of physiological doses of UVR (UVA + UVB) on quiescent melanocytes in vitro.</div> <div itemprodp="datePublished">Tue Sep 01 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/31461486"> <div itemprop="name">Human CAP cells represent a novel source for functional, miRNA-loaded exosome production.</div> </a> <div itemprop="about">PloS one</div> <div itemprop="author">Zeh N,Schneider H,Mathias S,Raab N,Kleemann M,Schmidt-Hertel S,Weis B,Wissing S,Strempel N,Handrick R,Otte K</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</div> <div itemprodp="datePublished">Tue Mar 03 00:00:00 EST 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30737083"> <div itemprop="name">A GBM-like V-ATPase signature directs cell-cell tumor signaling and reprogramming via large oncosomes.</div> </a> <div itemprop="about">EBioMedicine</div> <div itemprop="author">Bertolini I,Terrasi A,Martelli C,Gaudioso G,Di Cristofori A,Storaci AM,Formica M,Braidotti P,Todoerti K,Ferrero S,Caroli M,Ottobrini L,Vaccari T,Vaira V</div> <div itemprop="description">10626D was used in Western Blotting to study how expression of a GMB-like V-ATPase pump influences the non-neoplastic brain microenvironment.</div> <div itemprodp="datePublished">Fri Mar 01 00:00:00 EST 2019</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/31151295"> <div itemprop="name">Semaphorin-7A on Exosomes: A Promigratory Signal in the Glioma Microenvironment.</div> </a> <div itemprop="about">Cancers</div> <div itemprop="author">Manini I,Ruaro ME,Sgarra R,Bartolini A,Caponnetto F,Ius T,Skrap M,Di Loreto C,Beltrami AP,Manfioletti G,Cesselli D</div> <div itemprop="description">10626D was used in Western Blotting to suggest SEMA7A-β1-integrin as a new target to disrupt the communication between GSCs and the supporting microenvironment.</div> <div itemprodp="datePublished">Thu May 30 00:00:00 EDT 2019</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/29604274"> <div itemprop="name">Suppression of tau propagation using an inhibitor that targets the DK-switch of nSMase2.</div> </a> <div itemprop="about">Biochemical and biophysical research communications</div> <div itemprop="author">Bilousova T,Elias C,Miyoshi E,Alam MP,Zhu C,Campagna J,Vadivel K,Jagodzinska B,Gylys KH,John V</div> <div itemprop="description">10626D was used in Western Blotting to reveal that cambinol can target the DK-switch in the nSMase2 active site.</div> <div itemprodp="datePublished">Wed May 23 00:00:00 EDT 2018</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/29507392"> <div itemprop="name">Glycome analysis of extracellular vesicles derived from human induced pluripotent stem cells using lectin microarray.</div> </a> <div itemprop="about">Scientific reports</div> <div itemprop="author">Saito S,Hiemori K,Kiyoi K,Tateno H</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</div> <div itemprodp="datePublished">Mon Mar 05 00:00:00 EST 2018</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30260987"> <div itemprop="name">Efficient extracellular vesicle isolation by combining cell media modifications, ultrafiltration, and size-exclusion chromatography.</div> </a> <div itemprop="about">PloS one</div> <div itemprop="author">Guerreiro EM,Vestad B,Steffensen LA,Aass HCD,Saeed M,Øvstebø R,Costea DE,Galtung HK,Søland TM</div> <div itemprop="description">10626D was used in Western Blotting to develop a new combination of methods for the isolation of extracellular vesicles from cell culture without the use of specialised equipment.</div> <div itemprodp="datePublished">Mon Mar 04 00:00:00 EST 2019</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/29696078"> <div itemprop="name">Newt cells secrete extracellular vesicles with therapeutic bioactivity in mammalian cardiomyocytes.</div> </a> <div itemprop="about">Journal of extracellular vesicles</div> <div itemprop="author">Middleton RC,Rogers RG,De Couto G,Tseliou E,Luther K,Holewinski R,Soetkamp D,Van Eyk JE,Antes TJ,Marbán E</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</div> <div itemprodp="datePublished">Thu Oct 01 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30379855"> <div itemprop="name">Harmonization of exosome isolation from culture supernatants for optimized proteomics analysis.</div> </a> <div itemprop="about">PloS one</div> <div itemprop="author">Abramowicz A,Marczak L,Wojakowska A,Zapotoczny S,Whiteside TL,Widlak P,Pietrowska M</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</div> <div itemprodp="datePublished">Thu Apr 04 00:00:00 EDT 2019</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/30389917"> <div itemprop="name">Exosomes derived from HIV-1-infected cells promote growth and progression of cancer via HIV TAR RNA.</div> </a> <div itemprop="about">Nature communications</div> <div itemprop="author">Chen L,Feng Z,Yue H,Bazdar D,Mbonye U,Zender C,Harding CV,Bruggeman L,Karn J,Sieg SF,Wang B,Jin G</div> <div itemprop="description">Published figure using CD9 monoclonal antibody (Product # 10626D) in Western Blot</div> <div itemprodp="datePublished">Fri Nov 02 00:00:00 EDT 2018</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/29468680"> <div itemprop="name">Proinflammatory role of blister fluid-derived exosomes in bullous pemphigoid.</div> </a> <div itemprop="about">The Journal of pathology</div> <div itemprop="author">Fang H,Shao S,Jiang M,Dang E,Shen S,Zhang J,Qiao P,Li C,Wang G</div> <div itemprop="description">10626D was used in Western Blotting to provide strong evidence that blister fluid-derived exosomes are involved in the local autoinflammatory responses of the skin associated with bullous pemphigoid.</div> <div itemprodp="datePublished">Tue May 01 00:00:00 EDT 2018</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/28173719"> <div itemprop="name">Exosomal MicroRNA-15a Transfer from the Pancreas Augments Diabetic Complications by Inducing Oxidative Stress.</div> </a> <div itemprop="about">Antioxidants & redox signaling</div> <div itemprop="author">Kamalden TA,Macgregor-Das AM,Kannan SM,Dunkerly-Eyring B,Khaliddin N,Xu Z,Fusco AP,Yazib SA,Chow RC,Duh EJ,Halushka MK,Steenbergen C,Das S</div> <div itemprop="description">10626D was used in Western Blotting to determine the role of exosomal miRNA during type 2 diabetes.</div> <div itemprodp="datePublished">Wed Nov 01 00:00:00 EDT 2017</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/27699267"> <div itemprop="name">Quantitation of circulating satellite RNAs in pancreatic cancer patients.</div> </a> <div itemprop="about">JCI insight</div> <div itemprop="author">Kishikawa T,Otsuka M,Yoshikawa T,Ohno M,Yamamoto K,Yamamoto N,Kotani A,Koike K</div> <div itemprop="description">10626D was used in Western Blotting to develop a convenient and sensitive method for quantifying aberrantly expressed satellite repeat RNAs in sera.</div> <div itemprodp="datePublished">Thu Jun 02 00:00:00 EDT 2016</div> </div> </noscript> </div> <div class="border-bottom"></div> <div class="product-bioinformatics-details"> <div class="container"> <h2 class="pdp-sub-heading">Bioinformatics</h2> <div class="row"> <div class="large-span-6 medium-span-12 small-span-12"> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="proteinContent"> <strong>Protein Aliases:</strong> 5H9; 5H9 antigen; antigen CD9; BA-2/p24 antigen; BA2; CD9; CD9 antigen; CD9 antigen (p24); Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; motility related protein-1; Motility-related protein; MRP-1; p24; sCD 9; sCD9; soluble CD 9; soluble CD9; Tetraspanin-29; Tetraspanin29; Tspan-29 </p> <p class="view-buttons" id="ptnViewBtn" ng-class="$ctrl.bioContentLines('proteinContent', 'ptnViewBtn')"> <a id="proteinViewMoreBtn" ng-click="$ctrl.bioContentViewMore('proteinViewMoreBtn', 'proteinViewLessBtn', 'proteinContent')"> View more </a> <a id="proteinViewLessBtn" ng-click="$ctrl.bioContentViewLess('proteinViewLessBtn', 'proteinViewMoreBtn', 'proteinContent')"> View less </a> </p> </div> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="geneContent"> <strong>Gene Aliases:</strong> BTCC-1; CD9; DRAP-27; GIG2; MIC3; MRP-1; TSPAN-29; TSPAN29 </p> <p class="view-buttons" id="geneViewBtn" ng-class="$ctrl.bioContentLines('geneContent', 'geneViewBtn')"> <a id="geneViewMoreBtn" ng-click="$ctrl.bioContentViewMore('geneViewMoreBtn', 'geneViewLessBtn', 'geneContent')"> View more </a> <a id="geneViewLessBtn" ng-click="$ctrl.bioContentViewLess('geneViewLessBtn', 'geneViewMoreBtn', 'geneContent')"> View less </a> </p> </div> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="unitProContent"> <strong>UniProt ID:</strong> <a href="http://www.uniprot.org/uniprot/P21926" target="_blank">(Human) P21926</a> </p> <p class="view-buttons" id="unitViewBtn" ng-class="$ctrl.bioContentLines('unitProContent', 'unitViewBtn')"> <a id="unitProViewMoreBtn" ng-click="$ctrl.bioContentViewMore('unitProViewMoreBtn', 'unitProViewLessBtn', 'unitProContent')"> View more </a> <a id="unitProViewLessBtn" ng-click="$ctrl.bioContentViewLess('unitProViewLessBtn', 'unitProViewMoreBtn', 'unitProContent')"> View less </a> </p> </div> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="entrezContent"> <strong>Entrez Gene ID:</strong> <a href="http://www.ncbi.nlm.nih.gov/gene?term=928" target="_blank">(Human) 928</a> </p> <p class="view-buttons" id="etzViewBtn" ng-class="$ctrl.bioContentLines('entrezContent', 'etzViewBtn')"> <a id="entrezViewMoreBtn" ng-click="$ctrl.bioContentViewMore('entrezViewMoreBtn', 'entrezViewLessBtn', 'entrezContent')"> View more </a> <a id="entrezViewLessBtn" ng-click="$ctrl.bioContentViewLess('entrezViewLessBtn', 'entrezViewMoreBtn', 'entrezContent')"> View less </a> </p> </div> </div> <div class="large-span-6 medium-span-12 small-span-12"> <div class="accordion" id="accordion-bioinformatics"> <div class="accordion-group"> <div class="accordion-heading"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion2" target="_self" href="#collapseOne"> Function(s) </a> </div> <div id="collapseOne" class="accordion-body collapse"> <div class="accordion-inner"> <a class="icon" href="/antibody/primary/panther/integrin binding"> integrin binding </a> <a class="icon" href="/antibody/primary/panther/protein binding"> protein binding </a> </div> </div> </div> <div class="accordion-group"> <div class="accordion-heading"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion2" target="_self" href="#collapseTwo"> Process(es) </a> </div> <div id="collapseTwo" class="accordion-body collapse"> <div class="accordion-inner"> <a class="icon" href="/antibody/primary/panther/platelet degranulation"> platelet degranulation </a> <a class="icon" href="/antibody/primary/panther/movement of cell or subcellular component"> movement of cell or subcellular component </a> <a class="icon" href="/antibody/primary/panther/cell adhesion"> cell adhesion </a> <a class="icon" href="/antibody/primary/panther/cell surface receptor signaling pathway"> cell surface receptor signaling pathway </a> <a class="icon" href="/antibody/primary/panther/fusion of sperm to egg plasma membrane"> fusion of sperm to egg plasma membrane </a> <a class="icon" href="/antibody/primary/panther/brain development"> brain development </a> <a class="icon" href="/antibody/primary/panther/negative regulation of cell proliferation"> negative regulation of cell proliferation </a> <a class="icon" href="/antibody/primary/panther/response to water deprivation"> response to water deprivation </a> <a class="icon" href="/antibody/primary/panther/oligodendrocyte development"> oligodendrocyte development </a> <a class="icon" href="/antibody/primary/panther/platelet activation"> platelet activation </a> <a class="icon" href="/antibody/primary/panther/paranodal junction assembly"> paranodal junction assembly </a> <a class="icon" href="/antibody/primary/panther/receptor internalization"> receptor internalization </a> <a class="icon" href="/antibody/primary/panther/cellular response to low-density lipoprotein particle stimulus"> cellular response to low-density lipoprotein particle stimulus </a> </div> </div> </div> </div> </div> </div> </div> </div> <div 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