Annals of Biotechnology

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class="img-responsive col-md-12 col-sm-12"/> <div class="ab_class_02"> <h5>Annals of Biotechnology</h5> <p><strong>Short Name:</strong> Ann Biotechnol <br><strong>ISSN: </strong>2637-4927<br><strong>Publisher:</strong> MedDocs Publishers LLC</p> </div> </div> <div class="col-md-5 text-upper hidden-xs padding_0px media_div"> <div class="btns1 btns"> <a href="author-guidelines.html" class="col-md-6 ">Author Guidelines</a> <a href="submit-manuscript.php" class="col-md-6 mg_l_5">SUBMIT MANUSCRIPT</a> </div> <div class="scroll_txt padding_10"> <div class="holder"> <ul id="ticker01"> <li><a href=""> Proteomics</a></li> <li><a href=""> Cardiovascular Biomaterials</a></li> <li><a href=""> Biotechnology Applications</a></li> <li><a href=""> Biocatalysis</a></li> <li><a href=""> Pesticides</a></li> <li><a href=""> Red Biotechnology</a></li> <li><a href=""> Antibiotics</a></li> <li><a href=""> Genetic Manipulation</a></li> <li><a href=""> White Biotechnology</a></li> <li><a href=""> Enzymes</a></li> <li><a href=""> Blue Biotechnology</a></li> <li><a href=""> Green Biotechnology </a></li> <li><a href=""> Transgenic Plants</a></li> <li><a href=""> Modern Biotechnology</a></li> <li><a href=""> Bioinformatics </a></li> <li><a href=""> Functional Genomics</a></li> <li><a href=""> Structural Genomics</a></li> <li><a href=""> Gene Expression</a></li> <li><a href=""> Gene Therapy</a></li> <li><a href=""> Applied Biotechnology</a></li> <li><a href=""> Nano Biotechnology</a></li> </ul> </div> </div> </div> </div> <div class="col-md-12"> <h4 class="abt_joner"> <span class="span_class"> Archive</span></h4> <div> <div class="newtextbox1"> <h4>Development of DNA Aptamers that Detect Clonorchis Sinensis Adult Fluke and Egg Proteins by Enzyme-Linked Microplate Assay</h4> <h5>John G Bruno*; Jeffrey C Sivils</h5> <p>    Development of DNA aptamers through ten rounds of selection and PCR amplification (SELEX) against recombinant adult Cs44 and Egg proteins of Clonorchis sinensis liver flukes is described along with the top three aptamer DNA sequences for each target derived from next generation sequencing. ..</p> <div class="pull-right">  <a href="#X" target="_blank"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/development-of-dna-aptamers-that-detect-clonorchis-sinensis-adult-fluke-and-egg-proteins-by-enzyme-linked-microplate-assay.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="newtextbox1"> <h4>Recombinant DNA Technology Approaches to Words the Vaccination or Treatment Hepatitis B</h4> <h5>Hamadullah Soomro</h5> <p>    Recombinant DNA technology is a crucial method that involves separating and inserting a gene of interest into a specific vector. One important application of this technology is in combating Hepatitis B, a widespread infectious disease in humans, by developing effective vaccines and treatments. ..</p> <div class="pull-right">  <a href="#X" target="_blank"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/recombinant-dna-technology-approaches-to-words-the-vaccination-or-treatment-hepatitis-b.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="newtextbox1"> <h4>Coronavirus: The Picture it Showed, the Message We Chose to Ignore!</h4> <h5>Sudhakar Krittika; Pankaj Yadav*</h5> <p>    Nature needs to shake off the dust built due to its exploitation by humankind. One such heavy blow was probably the current COVID-19 pandemic. The COVID-19 pandemic has enabled habitat restoration of various wildlife and natural resources, along side the economic challenges it has imposed with increased poaching in certain countries ..</p> <div class="pull-right">  <a href="#X" target="_blank"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/coronavirus-the-picture-it-showed-the-message-we-chose-to-ignore.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="newtextbox1"> <h4>Network Analyses of the Inventory of Interpersonal Problems-32 and the Associations of its Dimensions with Oppositional Defiant Disorder Dimensions in Emerging Adults</h4> <h5>Rapson Gomez; Taylor Brown*</h5> <p>    Interpersonal relationship problems are key to Opposi tional Defiant Disorder (ODD). The study aimed to examine the types of interpersonal relationship problems, as pre sented in the Interpersonal Circumplex Model (IPC), with the ODD dimensions (oppositional, negative affect, and an tagonistic behavior) proposed by Burke et al. (2010).</p> <div class="pull-right">  <a href="annals-of-biotechnology/network-analyses-of-the-inventory-of-interpersonal-problems-32-and-the-associations-of-supplementary-tables.docx" target="_blank"><span class="label1 label-danger2"><i class="fa fa-file" aria-hidden="true"></i>  Supplementary Tables</span></a> <a href="annals-of-biotechnology/network-analyses-of-the-inventory-of-interpersonal-problems-32-and-the-associations-of-supplementary-figures.docx" target="_blank"><span class="label1 label-danger3"><i class="fa fa-image" aria-hidden="true"></i>  Supplementary Figures</span></a> <a href="#X" target="_blank"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/network-analyses-of-the-inventory-of-interpersonal-problems-32-and-the-associations-of-its-dimensions-with-oppositional-defiant-disorder-dimensions-in-emerging-adults.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="newtextbox1"> <h4>ApoB48 of Chylomicrons (CM) after Fat Load are Incorporated into the Liver and Reconstituted to VLDL ApoB48 in Humans</h4> <h5>Yoshiharu Tokita*; Katsuyuki Nakajima*; Akira Tanaka</h5> <p>    The majority of Remnant Lipoproteins (RLP) in the postprandial plasma have been believed to be chylomicron (CM) remnants (exogenous remnants; RLP-apoB48) derived from the intestine, not VLDL remnants (endogenous remnants; RLP-apoB100) derived from the liver ..</p> <div class="pull-right">  <a href="#X" target="_blank"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/apob48-of-chylomicrons-cm-after-fat-load-are-incorporated-into-the-liver-and-reconstituted-to-vldl-apob48-in-humans.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="newtextbox1"> <h4>Extraction of essential oil from lemon and orange peel by Clevenger apparatus: Comparative GC_MS analysis of chemical composition, from Debre Berehan Market town Amahara Region Ethiopia</h4> <h5>Zelalem Zayse Zeleke</h5> <p>    The peel of orange and lemon fruits is considered as a waste. It is used as a source of essential oils that can be applicable for food flavoring agent, pharmaceuticals, cosmetics, and soap and detergent factories. In this work, essential oils were extracted from lemon (1.56%) and orange (3.55%) peels by hydro distillation. The physiochemical properties of oils were recorded...</p> <div class="pull-right"> <a href="#X" data-toggle="modal" data-target="#crossref26" title=""><span class="label1 label-success"><i class="fa fa-code" aria-hidden="true"></i>  DOI</span> </a> <a href="annals-of-biotechnology/extraction-of-essential-oil-from-lemon-and-orange-peel-by-clevenger-apparatus.html" target="_blank"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/extraction-of-essential-oil-from-lemon-and-orange-peel-by-clevenger-apparatus.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="newtextbox1"> <h4>Two-peak Elution Profile of a Bispecific VHH-IgG Fusion Protein in Ion Exchange Purification Process caused by Atypical N-Glycosylation</h4> <h5>Jing Jiao; Liming Huang; Xiaohui Xu; Yuying Liu; Mingyan Hu; Weifeng Chen; Lei Li; Hui Xu; Xugang He; Nannan Li; Aihua Chen; Chenxi Shen; Lei Wang; Xun Liu; Lianshan Zhang; Chenxiang Tang*</h5> <p>    In-depth analysis and thorough understanding of N-glycosylation is critical for therapeutic proteins. For IgG or IgG-like molecules, the conserved N-glycosylation at Asn-297 has critical safety and efficacy implications and therefore is under close scrutiny. Any additional N-glycosylation that increases complexity and heterogeneity of the molecule, should be avoided at the early stage of drug discovery. However, unexpected glycosylation at atypical sites brings unforeseen challenges for antibody drug development process...</p> <div class="pull-right"> <a href="#X" data-toggle="modal" data-target="#crossref25" title=""><span class="label1 label-success"><i class="fa fa-code" aria-hidden="true"></i>  DOI</span> </a> <a href="annals-of-biotechnology/two-peak-elution-profile-of-a-bispecific-vhh-igg-fusion-protein-in-ion-exchange-purification.html" target="_blank"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/two-peak-elution-profile-of-a-bispecific-vhh-igg-fusion-protein-in-ion-exchange-purification.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="newtextbox1"> <h4>Optical Coherence Tomography <em>in Vivo</em> inLaryngology Clinics, an Overview</h4> <h5>Mette Pedersen*; Christian Larsen</h5> <p>    The aim of this review is to show the distance between the technical side of OCT and the understanding and needs of the laryngologists. In updated scientific research on OCT in laryngology, especially of the vocal folds, there are some obstacles of the techniques before the clinicians can use optical coherence tomography routinely.</p> <div class="pull-right"> <a href="#X" data-toggle="modal" data-target="#crossref24" title=""><span class="label1 label-success"><i class="fa fa-code" aria-hidden="true"></i>  DOI</span> </a> <a href="annals-of-biotechnology/optical-coherence-tomography-in-vivo-in-laryngology-clinics-an-overview.html" target="_blank"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/optical-coherence-tomography-in-vivo-in-laryngology-clinics-an-overview.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="newtextbox1"> <h4>Novel Ink - Stained Reflectance Confocal Microscopy Guided Pigmented Skin Lesion Biopsy Technique: Reducing Pathology Sample Bias Through Improved Precision</h4> <h5>Joanna Ludzik*; Claudia Lee; Alexander Witkowski; Jonathon Hetts; Sancy Leachman</h5> <p>    With skin cancer incidence rate steadily increasing each year, melanoma continues to pose a significant public health problem globally. It is well-known that early detection and prevention of melanoma remains the most important intervention for reducing morbidity and mortality, therefore it is imperative to promote innovation that will advance our ability to diagnose melanoma early...</p> <div class="pull-right"> <a href="#X" data-toggle="modal" data-target="#crossref23" title=""><span class="label1 label-success"><i class="fa fa-code" aria-hidden="true"></i>  DOI</span> </a> <a href="annals-of-biotechnology/novel-ink-stained-reflectance-confocalmicroscopy-guided-pigmented-skin-lesionbiopsy-technique-reducing-pathology-sample-bias-through-improved-precision.html" target="_blank"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/novel-ink-stained-reflectance-confocalmicroscopy-guided-pigmented-skin-lesionbiopsy-technique-reducing-pathology-sample-bias-through-improved-precision.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="newtextbox1"> <h4>Breeding Approaches for Disease Resistance in Crop Plants: A Review</h4> <h5>Shivam Maurya*; Mahabeer Singh; Sunil Kumar; Lalita Lakhran; Naresh Kumar; Satyadev Prajapati</h5> <p>    Present day agriculture has disturbed the age-vintage stability of host-pathogen system resulting diseases arise at regular interval leading to significant loss to the agricultural production, that is predicted to be 10-25% or much less in the course of everyday years however might also additionally enlarge as much as 100 % in the course of epidemic. Broadly, there are breeding tactics followed for disease resistance, which incorporates molecular and molecular one...</p> <div class="pull-right"> <a href="annals-of-biotechnology/breeding-approaches-for-disease-resistance-in-crop-plants-a-review.html" target="_blank"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/breeding-approaches-for-disease-resistance-in-crop-plants-a-review.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="newtextbox1"> <h4>Animal Models of PCOS: A Comprehensive Review on Current Advancement, Diet-Microbiota Interactions and Future Trend on Developing PCOS Induced Rodent Models</h4> <h5>Pritha Biswas; Pratip Chakraborty; Anindita Bhattacharya; Anamika Das; Mohan Das; Rintu Banerjee; Koel Chaudhury*</h5> <p>    Polycystic Ovary Syndrome (PCOS) accounts for a major form of dysovulatory infertility, often observed in ~15% women belonging to reproductive age. Being the etiology undecipherable and the management requiring a diversified approach, the call for a suitable animal model is warranted that will be able to mimic both reproductive as well as metabolic facets of the syndrome...</p> <div class="pull-right"> <a href="annals-of-biotechnology/animal-models-of-pcos-a-comprehensive-review-on-current-advancement-diet-microbiota-interactions-and-future-trend-on-developing-pcos-induced-rodent-models.html" target="_blank"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/animal-models-of-pcos-a-comprehensive-review-on-current-advancement-diet-microbiota-interactions-and-future-trend-on-developing-pcos-induced-rodent-models.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="newtextbox1"> <h4>Molecular Docking Study of different Natural Bio-Active Compounds of 'Swastha Kada Kashayam' on SARS-CoV-2 (nCoV) Proteins</h4> <h5>Kalpana S*; Suganthan R; Prabu P</h5> <p>    Background: Since the COVID-19 a pandemic disease, so far there are no drugs or other treatment practices currently approved by the U.S. Food and Drug Administration (FDA) to treat COVID-19 patients. Current scenario is only clinical management includes infection prevention and control measures and supportive care, including oxygen supplementation and mechanical ventilatory support when indicated...</p> <div class="pull-right"> <a href="#X"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/molecular-docking-study-of-different-natural-bio-active-compounds-of-swastha-kada-kashayam-on-sars-cov-2-ncov-proteins.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="newtextbox1"> <h4>Restrictive dermopathy: Novel <em>ZMPSTE24</em> mutation and clues for prenatal diagnosis</h4> <h5>Valentina Guida*; Agnese Giovannetti; Francesca Piceci-Sparascio; Tommaso Mazza; Enrica Marchionni; Lucia Menale; Gabriella Fatigante; Hossein Hozhabri; Alice Traversa; Viviana Caputo; Antonio Pizzuti; Alessandro Ferraris; Alessandro De Luca</h5> <p>    <strong>Background:</strong> Prenatal diagnosis of Restrictive Dermopathy (RD) in routine ultrasonography is particularly challenging since most fetal ultrasonography RD findings are non-specific and appear during late pregnancy.</p> <div class="pull-right"> <a href="annals-of-biotechnology/Restrictive-dermopathy-Novel-ZMPSTE24-mutation-and-clues-for-prenatal-diagnosis.html"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/Restrictive-dermopathy-Novel-ZMPSTE24-mutation-and-clues-for-prenatal-diagnosis.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="newtextbox1"> <h4>Microbial load in unpasteurized milk obtained from selected milk outlets in Kawempe division of Kampala, Uganda</h4> <h5>John N Kateregga; Collins Atuheire; Esther Nabatta; Samuel Majalija; James G Ndukui*</h5> <p>    <strong>Background:</strong> Milk consumption and demand has increased drastically over the past years in response to expanding human population in Uganda and world at large. Despite this, many residents of Kampala city obtain milk from shops supplied by milk vendors, who in turn, collect it directly from farms. The microbial load of this unpasteurized milk is not known, yet it could expose the population to milk-borne diseases..</p> <div class="pull-right"> <a href="annals-of-biotechnology/microbial-load-in-unpasteurized-milk-obtained-from-selected-milk-outlets-in-Kawempe-division-of-Kampala-Uganda.html"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/microbial-load-in-unpasteurized-milk-obtained-from-selected-milk-outlets-in-Kawempe-division-of-Kampala-Uganda.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="newtextbox1"> <h4>Detection of antibodies anti-gp43 of <em>Paracoccidioides brasiliensis</em> in sera samples by double-sandwich ELISA</h4> <h5>Luís Felipe Minozzo Figueiredo; Barbara Ribeiro Fonseca-de-Souza; Alessandra Becker-Finco; Elizângela Almeida Rocha; Luísa Lasmar Lima Oliveira; Ricardo Machado-de-Ávila; João Carlos Minozzo; Fernanda Costal-Oliveira; Larissa Magalhães Alvarenga; Vanete Soccol; Carlos Chávez-Olórtegui*</h5> <p>    Monoclonal antibodies (mAbs) were raised against 43 kDa glycoprotein (gp43) of <em>Paracoccidioides brasiliensis</em>. One mAb anti-gp43 and gp43 were used in a double-antibody sandwich ELISA to detect antibodies in sera from patients with Paracoccidioidomycosis. This ELISA diagnosis showed 91.18% sensibility and 100.0% specificity against sera of patients with histoplasmosis, aspergillosis or chromomycosis.</p> <div class="pull-right"> <a href="annals-of-biotechnology/detection-of-antibodies-anti-gp43-of-paracoccidioides-brasiliensis-in-sera-samples-by-double-sandwich-ELISA.html"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/detection-of-antibodies-anti-gp43-of-paracoccidioides-brasiliensis-in-sera-samples-by-double-sandwich-ELISA.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="newtextbox1"> <h4>Effect of antiretroviral drugs on the renal function of HIV seropositive patients attending ISS clinic of Mulago Hospital, Uganda</h4> <h5>John N Kateregga; Collins Atuheire; Justine Nalunga; Ndukui G James*</h5> <p>    Background: Highly Active Antiretroviral Therapy (HAART) consisting of at least three active drugs against HIV infection has improved HIV/AIDS management since their inception. However, alterations of lipid metabolism due to HAART can result into secondary diabetic and hypertensive renal damage. This study was carried out to assess the effect of antiretroviral drugs on the renal function of HIV seropositive patients by determining the serum levels of Creatinine, Urea and Electrolytes (Na, K, and Cl) of the patients on treatment.</p> <div class="pull-right"> <a href="annals-of-biotechnology/effect-of-antiretroviral-drugs-on-the-renal-function-of-HIV-seropositive-patients-attending-ISS-clinic-of-Mulago-Hospital-Uganda.html"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/effect-of-antiretroviral-drugs-on-the-renal-function-of-HIV-seropositive-patients-attending-ISS-clinic-of-Mulago-Hospital-Uganda.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="newtextbox1"> <h4>Anti-aging effects of the proteins from artemia extract on human fibroblasts cell proliferation and collagen expression in induced aging conditions</h4> <h5>Neda Vaseli-Hagh; Abdolkhaleg Deezagi*; Mahvash Khodabandeh Shahraki</h5> <p>    Researches on the factors that inhibit senescence are attractive in health industries. Artemia extract uses in cosmetic and sunlight protecting products. The aim of this work was to study the effects of Artemia extract on the cell proliferation, senescence and collagen synthesis by human fibroblasts in normal and aging induced conditions.</p> <div class="pull-right"> <a href="#X" data-toggle="modal" data-target="#crossref15" title=""><span class="label1 label-success"><i class="fa fa-code" aria-hidden="true"></i>  DOI</span> </a> <a href="annals-of-biotechnology/anti-aging-effects-of-the-proteins-from-artemia-extract-on-human-fibroblasts-cell-proliferation-and-collagen-expression-in-induced-aging-conditions.html"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/anti-aging-effects-of-the-proteins-from-artemia-extract-on-human-fibroblasts-cell-proliferation-and-collagen-expression-in-induced-aging-conditions.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="newtextbox1"> <h4>Somatic mutations in IFN-γ-Signal molecules in human uterine leiomyosarcoma</h4> <h5>Takuma Hayashi*; Tomoyuki Ichimura; Mari Kasai; Kenji Sano; Susumu Tonegawa; Yae Kanai; Dorit Zharhary; Hiroyuki Aburatani; Nobuo Yaegashi; Ikuo Konishi</h5> <p>    Uterus Leiomyosarcoma (uLMS), unfortunately, is a disease with a poor prognosis. In the report of the Ministry of Health, Labor and Welfare, the 50% survival time (mOS) summarized from Stage I to Stage IV was 31months. Preoperative diagnosis of uLMS is very difficult. Although it is diagnosed as "uterine fibroids", it is not uncommon in cases where the uterine leiomyosarcoma is definitely diagnosed again after surgical hysterectomy or removal of myoma.</p> <div class="pull-right"> <a href="#X" data-toggle="modal" data-target="#crossref14" title=""><span class="label1 label-success"><i class="fa fa-code" aria-hidden="true"></i>  DOI</span> </a> <a href="annals-of-biotechnology/somatic-mutations-in-IFN-Signal-molecules-in-human-uterine-leiomyosarcoma.html"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/somatic-mutations-in-IFN-γ-Signal-molecules-in-human-uterine-leiomyosarcoma.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="newtextbox1"> <h4>Modern biotechnology: Origination of paper-based analytical devices</h4> <h5>Shilpa Sivashankar*; Prasad Salunke</h5> <p>    Nowadays most of the biotechnological processes can be performed on benchtop platforms without the need for an expensive, space-consuming equipment. In addition, the sensitivity and specificity of the conventional assays using biotechnological processes can be improved with the recent advancements in materials science and modern technology.</p> <div class="pull-right"> <a href="#X" data-toggle="modal" data-target="#crossref13" title=""><span class="label1 label-success"><i class="fa fa-code" aria-hidden="true"></i>  DOI</span> </a> <a href="annals-of-biotechnology/modern-biotechnology-origination-of-paper-based-analytical-devices.html"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/modern-biotechnology-origination-of-paper-based-analytical-devices.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="newtextbox1"> <h4>The Future of 'Self-Healing' Technologies</h4> <h5>KOH Leng-Duei</h5> <p>    Recent years have witnessed significant advancement in 'self-healing' technologies. This is unlike a decade back, when 'self-healing' materials which are capable of restoring functionalities such as electrical conductivity or even optical (e.g., display) property after mechanical damage were practically non-existent. This editorial provides a quick introduction on reported 'self-healing' technologies,...</p> <div class="pull-right"> <a href="#X" data-toggle="modal" data-target="#crossref12" title=""><span class="label1 label-success"><i class="fa fa-code" aria-hidden="true"></i>  DOI</span> </a> <a href="annals-of-biotechnology/the-future-of-self-Healing-technologies.html"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/the-future-of-self-Healing-technologies.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="newtextbox1"> <h4>Honey incorporated antibacterial acellular dermal matrix for full-thickness wound healing</h4> <h5>Archna Dhasmana*; Lubhan Singh; Partha Roy; Narayan Chandra Mishra</h5> <p>    Honey is a potent natural antibacterial remedy used for the treatment of chronic wounds. In this study, Honey incorporated Acellular Dermal Matrix (H-AGDM) was fabricated for accelerating the wound healing process. The physiochemical and morphological characterization of the fabricated matrix was performed by FTIR, FESEM, in vitro degradation study, and antibacterial activity.</p> <div class="pull-right"> <a href="#X" data-toggle="modal" data-target="#crossref11" title=""><span class="label1 label-success"><i class="fa fa-code" aria-hidden="true"></i>  DOI</span> </a> <a href="annals-of-biotechnology/honey-incorporated-antibacterial-acellular-dermal-matrix-for.html"><span class="label1 label-primary"><i class="fa fa-code" aria-hidden="true"></i>  HTML</span> </a> <a href="annals-of-biotechnology/honey-incorporated-antibacterial-acellular-dermal-matrix-for.pdf" target="_blank"><span class="label1 label-danger"><i class="fa fa-file-pdf-o" aria-hidden="true"></i>  PDF</span></a> </div> <div class="clearfix"></div> </div> <hr> <div class="divide-351"> <div style="text-align:center; font-size:18px;"> <ul class="pagination"> <li class="active"><a href="annals-of-biotechnology-archive.html">1</a></li> <li ><a href="annals-of-biotechnology-archive-2.html">2</a></li>  </ul> </div> </div> </div> </div> </div> </div> <div class="row"> <div class="col-md-12 text-upper display_none"> <div class="btns1 btns"> <a href="" class="col-md-6 col-xs-12">Author Guidelines</a> <a href="" class="col-md-6 mg_l_5 col-xs-12">SUBMIT MANUSCRIPT</a> </div> <div class="scroll_txt padding_10"> <div class="holder"> <ul id="ticker01"> <li><a href=""> Proteomics</a></li> <li><a href=""> Cardiovascular Biomaterials</a></li> <li><a href=""> Biotechnology Applications</a></li> <li><a href=""> Biocatalysis</a></li> <li><a href=""> Pesticides</a></li> <li><a href=""> Red Biotechnology</a></li> <li><a href=""> Antibiotics</a></li> <li><a href=""> Genetic Manipulation</a></li> <li><a href=""> White Biotechnology</a></li> <li><a href=""> Enzymes</a></li> <li><a href=""> Blue Biotechnology</a></li> <li><a href=""> Green Biotechnology </a></li> <li><a href=""> Transgenic Plants</a></li> <li><a href=""> Modern Biotechnology</a></li> <li><a href=""> Bioinformatics </a></li> <li><a href=""> Functional Genomics</a></li> <li><a href=""> Structural Genomics</a></li> <li><a href=""> Gene Expression</a></li> <li><a href=""> Gene Therapy</a></li> <li><a href=""> Applied Biotechnology</a></li> <li><a href=""> Nano Biotechnology</a></li> </ul> </div> </div> </div> </div> </div> </div> <div class="divide60"></div> <div class="search-filter"> <div class="container"> <div class="row"> <div class="col-md-10 col-xs-12"> <h3>MedDocs Publishers</h3> <p>We always work towards offering the best to you. 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Lubhan Singh2 ; Partha Roy3 ; Narayan Chandra Mishra1</em></strong> <br><sup>1</sup>Department of Polymer & Process Engineering, Indian Institute of Technology Roorkee <br><sup>2</sup>Department of Pharmacology, RIVET, Greater Noida <br><sup>3</sup>Department of Biotechnology, Indian Institute of Technology Roorkee </p> <p>Received: Jun 26, 2018<br> Accepted: Aug 15, 2018<br> Published Online: Aug 22, 2018</p> </div> </div> </div> <div class="modal-footer"> <button type="button" class="btn btn-default" data-dismiss="modal">Close</button> </div> </div> </div> </div>  <div id="crossref12" class="modal fade bs-example-modal-lg" role="dialog"> <div class="modal-dialog modal-lg"> <Modal content> <div class="modal-content modal-lg" style="width:800px;"> <div class="modal-header" style="padding:0;"> <button type="button" class="close" data-dismiss="modal">×</button> </div> <div class="doi-cf"><img src="img/crossref_logo.png" class="center"></div> <div class="modal-body" style="margin-left:20px;"> <section class="document-metadata"> <h5 class="document-metadata__text" style="line-height: 24px;">The Future of ‘Self-Healing’ Technologies</h5> <br> <p class="document-metadata__text"> Crossref DOI link: <a href="http://doi.org/10.33582/2637-4927/1012" target="_blank">http://doi.org/10.33582/2637-4927/1012</a> </p> </section> <div class="cross-ref-accordian"> <button class="accordion">More Information</button> <div class="panel"> <p><strong>Authors</strong></p> <p><strong><em>KOH Leng-Duei</em></strong> <br>Institute of Materials Research and Engineering, A*STAR, 2 Fusionopolis Way, Innovis, 138634, Singapore </p> <p>Received: May 30, 2018<br> Accepted: Aug 27, 2018<br> Published Online: Aug 30, 2018</p> </div> </div> </div> <div class="modal-footer"> <button type="button" class="btn btn-default" data-dismiss="modal">Close</button> </div> </div> </div> </div>  <div id="crossref13" class="modal fade bs-example-modal-lg" role="dialog"> <div class="modal-dialog modal-lg"> <Modal content> <div class="modal-content modal-lg" style="width:800px;"> <div class="modal-header" style="padding:0;"> <button type="button" class="close" data-dismiss="modal">×</button> </div> <div class="doi-cf"><img src="img/crossref_logo.png" class="center"></div> <div class="modal-body" style="margin-left:20px;"> <section class="document-metadata"> <h5 class="document-metadata__text" style="line-height: 24px;">Modern biotechnology: Origination of paper-based analytical devices</h5> <br> <p class="document-metadata__text"> Crossref DOI link: <a href="http://doi.org/10.33582/2637-4927/1013" target="_blank">http://doi.org/10.33582/2637-4927/1013</a> </p> </section> <div class="cross-ref-accordian"> <button class="accordion">More Information</button> <div class="panel"> <p><strong>Authors</strong></p> <p><strong><em>Shilpa Sivashankar1*; Prasad Salunke2</em></strong> <br><sup>1</sup>Assistant professor, Department of Biotechnology, Acharya Institute of Technology, Bangalore, India <br><sup>2</sup>Assistant Professor, Department of Mechanical Engineering, Acharya Institute of Technology, Bangalore, India </p> <p>Received: May 03, 2018<br> Accepted: Sep 24, 2018<br> Published Online: Sep 27, 2018</p> </div> </div> </div> <div class="modal-footer"> <button type="button" class="btn btn-default" data-dismiss="modal">Close</button> </div> </div> </div> </div>  <div id="crossref14" class="modal fade bs-example-modal-lg" role="dialog"> <div class="modal-dialog modal-lg"> <Modal content> <div class="modal-content modal-lg" style="width:800px;"> <div class="modal-header" style="padding:0;"> <button type="button" class="close" data-dismiss="modal">×</button> </div> <div class="doi-cf"><img src="img/crossref_logo.png" class="center"></div> <div class="modal-body" style="margin-left:20px;"> <section class="document-metadata"> <h5 class="document-metadata__text" style="line-height: 24px;">Somatic mutations in IFN-γ-Signal molecules in human uterine leiomyosarcoma</h5> <br> <p class="document-metadata__text"> Crossref DOI link: <a href="http://doi.org/10.33582/2637-4927/1014" target="_blank">http://doi.org/10.33582/2637-4927/1014</a> </p> </section> <div class="cross-ref-accordian"> <button class="accordion">More Information</button> <div class="panel"> <p><strong>Authors</strong></p> <p><strong><em>Takuma Hayashi1,9,*; Tomoyuki Ichimura2 ; Mari Kasai2 ; Kenji Sano3 ; Susumu Tonegawa4 ; Yae Kanai5,10; Dorit Zharhary6,9; Hiroyuki Aburatani7 ; Nobuo Yaegashi8 ; Ikuo Konishi1</em></strong> <br><sup>1</sup>National Hospital Organization Kyoto Medical Center, Japan <br><sup>2</sup>Department of Obstetrics and Gynecology, Osaka City University, Japan <br><sup>3</sup>Pathology Division, Iida City Hospital, Japan 4 Picower Institute, Massachusetts Institute of Technology, USA <br><sup>4</sup>Picower Institute, Massachusetts Institute of Technology, USA <br><sup>5</sup>Pathology Division, Keio University School of Medicine, Japan <br><sup>6</sup>SIGMA-Aldrich Israel, Israel <br><sup>7</sup>Research Center for Advanced Science and Technology, The University of Tokyo, Japan <br><sup>8</sup>Department of Obstetrics and Gynecology, Tohoku University, Graduate School of Medicine, Japan <br><sup>9</sup>SIGMA-Aldrich Collaboration Lab and Promoting Business using Advanced Technology, Japan <br><sup>10</sup>The International Human Epigenom Consortium (IHEC) and CREST, Japan </p> <p>Received: Aug 22, 2018<br> Accepted: Sep 25, 2018<br> Published Online: Sep 28, 2018</p> </div> </div> </div> <div class="modal-footer"> <button type="button" class="btn btn-default" data-dismiss="modal">Close</button> </div> </div> </div> </div>  <div id="crossref15" class="modal fade bs-example-modal-lg" role="dialog"> <div class="modal-dialog modal-lg"> <Modal content> <div class="modal-content modal-lg" style="width:800px;"> <div class="modal-header" style="padding:0;"> <button type="button" class="close" data-dismiss="modal">×</button> </div> <div class="doi-cf"><img src="img/crossref_logo.png" class="center"></div> <div class="modal-body" style="margin-left:20px;"> <section class="document-metadata"> <h5 class="document-metadata__text" style="line-height: 24px;">Anti-aging effects of the proteins from artemia extract on human fibroblasts cell proliferation and collagen expression in induced aging conditions</h5> <br> <p class="document-metadata__text"> Crossref DOI link: <a href="http://doi.org/10.33582/2637-4927/1015" target="_blank">http://doi.org/10.33582/2637-4927/1015</a> </p> </section> <div class="cross-ref-accordian"> <button class="accordion">More Information</button> <div class="panel"> <p><strong>Authors</strong></p> <p><strong><em>Neda Vaseli-Hagh1 ; Abdolkhaleg Deezagi*1 ; Mahvash Khodabandeh Shahraki2</em></strong> <br><sup>1</sup>Department of molecular medicine & Biochemistry, National Institute of Genetic Engineering and Biotechnology, Tehran-Iran. <br><sup>2</sup>Department of Technology & Environment, National Institute of Genetic Engineering and Biotechnology, Tehran-Iran </p> <p>Received: Aug 29, 2018<br> Accepted: Oct 02, 2018<br> Published Online: Oct 05, 2018</p> </div> </div> </div> <div class="modal-footer"> <button type="button" class="btn btn-default" data-dismiss="modal">Close</button> </div> </div> </div> </div>  <div id="crossref26" class="modal fade bs-example-modal-lg" role="dialog"> <div class="modal-dialog modal-lg"> <Modal content> <div class="modal-content modal-lg" style="width:800px;"> <div class="modal-header" style="padding:0;"> <button type="button" class="close" data-dismiss="modal">×</button> </div> <div class="doi-cf"><img src="img/crossref_logo.png" class="center"></div> <div class="modal-body" style="margin-left:20px;"> <section class="document-metadata"> <h5 class="document-metadata__text" style="line-height: 24px;">Extraction of essential oil from lemon and orange peel by Clevenger apparatus: Comparative GC_MS analysis of chemical composition, from Debre Berehan Market town Amahara Region Ethiopia</h5> <br> <p class="document-metadata__text"> Crossref DOI link: <a href="https://doi.org/10.33582/AnnBiotechnol.2022.1026" target="_blank">https://doi.org/10.33582/AnnBiotechnol.2022.1026</a> </p> </section> <div class="cross-ref-accordian"> <button class="accordion">More Information</button> <div class="panel"> <p><strong>Authors</strong></p> <p><strong><em>Zelalem Zayse Zeleke</em></strong> <br>Wachemo University, Durame campus, Department of chemistry, Ethiopia. </p> <p>Received: May 19, 2022<br> Accepted: Jun 14, 2022<br> Published Online: Jun, 16, 2022</p> </div> </div> </div> <div class="modal-footer"> <button type="button" class="btn btn-default" data-dismiss="modal">Close</button> </div> </div> </div> </div>  <div id="crossref25" class="modal fade bs-example-modal-lg" role="dialog"> <div class="modal-dialog modal-lg"> <Modal content> <div class="modal-content modal-lg" style="width:800px;"> <div class="modal-header" style="padding:0;"> <button type="button" class="close" data-dismiss="modal">×</button> </div> <div class="doi-cf"><img src="img/crossref_logo.png" class="center"></div> <div class="modal-body" style="margin-left:20px;"> <section class="document-metadata"> <h5 class="document-metadata__text" style="line-height: 24px;">Two-peak Elution Profile of a Bispecific VHH-IgG Fusion Protein in Ion Exchange Purification Process caused by Atypical N-Glycosylation</h5> <br> <p class="document-metadata__text"> Crossref DOI link: <a href="https://doi.org/10.33582/AnnBiotechnol.2022.1025" target="_blank">https://doi.org/10.33582/AnnBiotechnol.2022.1025</a> </p> </section> <div class="cross-ref-accordian"> <button class="accordion">More Information</button> <div class="panel"> <p><strong>Authors</strong></p> <p><strong><em>Jing Jiao<sup>1</sup>; Liming Huang<sup>1</sup>; Xiaohui Xu<sup>1</sup>; Yuying Liu<sup>2</sup>; Mingyan Hu<sup>1</sup>; Weifeng Chen<sup>1</sup>; Lei Li<sup>1</sup>; Hui Xu<sup>1</sup>; Xugang He<sup>2</sup>; Nannan Li<sup>2</sup>; Aihua Chen<sup>1</sup>; Chenxi Shen<sup>2</sup>; Lei Wang<sup>2</sup>; Xun Liu<sup>3</sup>; Lianshan Zhang<sup>4</sup>; Chenxiang Tang<sup>1</sup>*</em></strong> <br><sup>1</sup>Shanghai MabGen Biotech Ltd, China. <br><sup>2</sup>Beijing Tuojie Biotech Co Ltd, China <br><sup>3</sup>Shanghai Hengrui Pharmaceuticals Co Ltd, China <br><sup>4</sup>Jiangsu Hengrui Pharmaceuticals Co Ltd, China. </p> <p>Received: Apr 12, 2022<br> Accepted: May 07, 2022<br> Published Online: May, 09, 2022</p> </div> </div> </div> <div class="modal-footer"> <button type="button" class="btn btn-default" data-dismiss="modal">Close</button> </div> </div> </div> </div>  <div id="crossref24" class="modal fade bs-example-modal-lg" role="dialog"> <div class="modal-dialog modal-lg"> <Modal content> <div class="modal-content modal-lg" style="width:800px;"> <div class="modal-header" style="padding:0;"> <button type="button" class="close" data-dismiss="modal">×</button> </div> <div class="doi-cf"><img src="img/crossref_logo.png" class="center"></div> <div class="modal-body" style="margin-left:20px;"> <section class="document-metadata"> <h5 class="document-metadata__text" style="line-height: 24px;">Optical Coherence Tomography <em>in Vivo</em> in Laryngology Clinics, an Overview</h5> <br> <p class="document-metadata__text"> Crossref DOI link: <a href="https://doi.org/10.33582/AnnBiotechnol.2022.1024" target="_blank">https://doi.org/10.33582/AnnBiotechnol.2022.1024</a> </p> </section> <div class="cross-ref-accordian"> <button class="accordion">More Information</button> <div class="panel"> <p><strong>Authors</strong></p> <p><strong><em>Mette Pedersen<sup>1</sup>*; Christian Larsen<sup>2</sup></em></strong> <br><sup>1</sup>ENT-specialist, Medical Centre, Østergade 18, 1100, Denmark. <br><sup>2</sup> Stud. Can (it), Copenhagen Business School, Solbjerg plads 3, 2000, Denmark. </p> <p>Received: Feb 10, 2022<br> Accepted: Feb 28, 2022<br> Published Online: Mar 04, 2022</p> </div> </div> </div> <div class="modal-footer"> <button type="button" class="btn btn-default" data-dismiss="modal">Close</button> </div> </div> </div> </div>  <div id="crossref23" class="modal fade bs-example-modal-lg" role="dialog"> <div class="modal-dialog modal-lg"> <Modal content> <div class="modal-content modal-lg" style="width:800px;"> <div class="modal-header" style="padding:0;"> <button type="button" class="close" data-dismiss="modal">×</button> </div> <div class="doi-cf"><img src="img/crossref_logo.png" class="center"></div> <div class="modal-body" style="margin-left:20px;"> <section class="document-metadata"> <h5 class="document-metadata__text" style="line-height: 24px;">Novel Ink - Stained Reflectance Confocal Microscopy Guided Pigmented Skin Lesion Biopsy Technique: Reducing Pathology Sample Bias Through Improved Precision</h5> <br> <p class="document-metadata__text"> Crossref DOI link: <a href="https://doi.org/10.33582/AnnBiotechnol.2022.1023" target="_blank">https://doi.org/10.33582/AnnBiotechnol.2022.1023</a> </p> </section> <div class="cross-ref-accordian"> <button class="accordion">More Information</button> <div class="panel"> <p><strong>Authors</strong></p> <p><strong><em>Joanna Ludzik<sup>1,2</sup>*; Claudia Lee<sup>2,3</sup>; Alexander Witkowski<sup>2</sup>; Jonathon Hetts<sup>2</sup>; Sancy Leachman<sup>2</sup></em></strong> <br><sup>1</sup>Department of Telemedicine and Bioinformatics, Jagiellonian University Medical College, Krakow, Poland. <br><sup>2</sup> Department of Dermatology, Oregon Health and Sciences University, Portland, Oregon, USA. <br><sup>3</sup> School of Medicine, University of California Riverside, Riverside, California, USA. </p> <p>Received: Jan 22, 2022<br> Accepted: Feb 15, 2022<br> Published Online: Feb 18, 2022</p> </div> </div> </div> <div class="modal-footer"> <button type="button" class="btn btn-default" data-dismiss="modal">Close</button> </div> </div> </div> </div>   <div id="lightbox" class="modal fade" tabindex="-1" role="dialog" aria-labelledby="myLargeModalLabel" aria-hidden="true"> <div class="modal-dialog"> <button type="button" class="close hidden" data-dismiss="modal" aria-hidden="true">×</button> <div class="modal-content"> <div class="modal-body"> <img src="" alt="" /> </div> </div> </div> </div>  <a href="javascript:" id="return-to-top"><i class="fa fa-arrow-up" aria-hidden="true"></i></a> <script src="js/jquery.min.js"></script> <script src='js/jquery-hover.js'></script> <script src="js/jquery-migrate.min.js"></script>  <script src="js/wow.min.js" type="text/javascript"></script>  <script src="bootstrap/js/bootstrap.min.js" type="text/javascript"></script>  <script src="js/jquery.easing.1.3.min.js" type="text/javascript"></script>  <script type="text/javascript" src="js/jquery.sticky.js"></script>  <script src="js/jquery.flexslider-min.js" type="text/javascript"></script>  <script src="js/jquery.stellar.min.js" type="text/javascript"></script>  <script src="js/owl.carousel.min.js" type="text/javascript"></script> <script src="js/classie.js" type="text/javascript"></script> <script src="js/selectFx.js" type="text/javascript"></script>  <script src="rs-plugin/js/jquery.themepunch.tools.min.js" type="text/javascript"></script> <script src="rs-plugin/js/jquery.themepunch.revolution.min.js" type="text/javascript"></script> <script src="js/tweetie.min.js" type="text/javascript"></script>  <script src="js/real-estate-custom.js" type="text/javascript"></script> <script type="text/javascript" src="js/hover-effect.js"></script> <script type="text/javascript"> /* $(function(){ $(".dropdown").hover( function() { $('.dropdown-menu', this).stop( true, true ).fadeIn("fast"); 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