ExCellerate Media for Immune and Stem Cell Culture Expansion

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<div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2>Serum-Free Expansion Media for Stem Cells and Immune Cells</h2> <p>ExCellerate Cell Expansion Media provide an optimized serum-free culture environment for the generation and expansion of individual immune cell types and induced pluripotent stem cells (iPSCs).</p> <ul> <li>Robust expansion and maintenance of cell cultures</li> <li>Reduce variability in your cell cultures</li> <li>Streamline comparability testing if raw material changes are required</li> <li>Cross-reference Drug Master Files to facilitate regulatory compliance</li> <li>Reduce time-to-market for your cell therapy</li> </ul> <p>Each production lot of ExCellerate media is thoroughly tested for consistent quality and performance.</p></div> </div> </div> <div> <div class="paragraph paragraph--type--button-row paragraph--view-mode--default"> <div class="row"> <div class="col"> <div class="list-grid-wrap"> <div class="row"> <div class="col-md-4"> <a href="#Immune Cell Expansion Media" class="btn btn-primary" type="link"> View Immune Cell Expansion Media </a> </div> <div class="col-md-4"> <a href="#iPSC Expansion Media" class="btn btn-primary" type="link"> View iPSC Expansion Media </a> </div> <div class="col-md-4"> <a href="#request-form" class="btn btn-primary" type="link"> Request Media Sample </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="Immune Cell Expansion Media"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2>Immune Cell Expansion Media</h2> <p>Mitigating risk and embedding flexibility into your cell culture process will improve process reproducibility and accelerate your immune cell therapy development. These ExCellerate cell expansion media are optimized for specific cell types and are key to building a culture process that ensures high yields and high quality.</p></div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><br><br><h3>ExCellerate Human NK Cell Expansion Media</h3></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><ul> <li>Made from completely animal-free raw materials</li> <li>Serum-free and phenol red-free formulation</li> <li>Maintains high fold expansion and killing capacity</li> <li>Enables restimulation without exhaustion</li> </ul></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="/p/cell-culture/excellerate-human-nk-cell-expansion-media-animal-component-free_ccm037" class="btn btn-primary" type="link"> View NK Cell Media </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/products/excellerate-human-nk-cell-expansion-media-525x315.png" alt="ExCellerate Human NK Cell Expansion Media" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="thin-banner banner_wrapper"> <div class="banner-light"> <div> <div><h3>Test ExCellerate Cell Expansion Media With Your Cells.</h3></div> <a href="#request-form" > <button type="button" class="btn btn-primary"> Request a Sample </button> </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h4>View ExCellerate NK Cell Expansion Media Data</h4></div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-nk-cells-feeder-free-growth-comparison25" role="button" aria-expanded="false" aria-controls="expPara"> <h5>NK Cells Feeder-Free Growth Comparison</h5> </a> </div> <div class="collapse" id="expPara-nk-cells-feeder-free-growth-comparison25" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/nk-cells-feeder-free-growth-comparison-graph.png" alt="NK Cells Feeder-Free Growth Comparison Graph" loading="lazy" > </div> <br/> <div><p>Purified NK cells from PBMCs of 3 donors were grown in a feeder-free process on G-Rex® plates over a 14-day culture, according to <a href="https://resources.bio-techne.com/bio-techne-assets/docs/literature/feeder-free-protocol_CCM037.pdf" target="_blank">this protocol.</a> ExCellerate NK Media (Catalog # <a href="/p/cell-culture/excellerate-human-nk-cell-expansion-media-animal-component-free_ccm037">CCM037</a>) outperformed other media suppliers, showing greater NK cell growth under feeder-free conditions compared to other NK cell expansion media.</p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-nk-cells-feeder-free-serum-free-growth-comparison18" role="button" aria-expanded="false" aria-controls="expPara"> <h5>NK Cells Feeder-Free, Serum-Free Growth Comparison</h5> </a> </div> <div class="collapse" id="expPara-nk-cells-feeder-free-serum-free-growth-comparison18" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/nk-cells-feeder-serum-free-growth-comparison-graph.png" alt="NK Cells Feeder-Free Serum-Free Growth Comparison Graph" loading="lazy" > </div> <br/> <div><p>Purified NK cells from PBMCs were grown in a feeder-free, serum-free process over a 14-day culture, according to <a href="https://resources.bio-techne.com/bio-techne-assets/docs/literature/feeder-free-protocol_CCM037.pdf" target="_blank">this protocol.</a> Only ExCellerate NK Cell Expansion Media (Catalog # <a href="/p/cell-culture/excellerate-human-nk-cell-expansion-media-animal-component-free_ccm037">CCM037</a>) allowed for the feeder-free growth of NK cells without serum.</p></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="d-flex flex-column h-100"> <a href="/p/cell-culture/excellerate-b-cell-media-xeno-free_ccm031" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/banners/teaser-bkgd-rounded-gray-500x200-v3.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/products/excellerate-b-cell-media-525x315.png" alt="ExCellerate B Cell Media" loading="lazy" > </div> </a> <a class="card_title_link" href="/p/cell-culture/excellerate-b-cell-media-xeno-free_ccm031" > <h3 class="card_title_htag"> <div><h3>ExCellerate B Cell Media</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><ul> <li>Xeno-free formulation for <em>ex vivo </em>culture of B lymphocytes</li> <li>Robust expansion of B cells from peripheral blood in multiple species</li> <li>Supports antigen-specific B cell clonogenicity and hybridoma culture</li> </ul></div> </div> <div class="card-bottom-link"> <div> <a href="/p/cell-culture/excellerate-b-cell-media-xeno-free_ccm031" > View B Cell Media </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="d-flex flex-column h-100"> <a href="/p/cell-culture/gmp-human-t-cell-media_ccm038-gmp" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/banners/teaser-bkgd-rounded-gray-500x200-v3.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/human-gmp-fg525x350v2.png" alt="Human GMP Vial" loading="lazy" > </div> </a> <a class="card_title_link" href="/p/cell-culture/gmp-human-t-cell-media_ccm038-gmp" > <h3 class="card_title_htag"> <div><h3>GMP Human T Cell Media</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><ul> <li>Clinic-ready formulation</li> <li>Robust T cell expansion from PBMCs or purified T cells</li> <li>Adopted by academic and biopharma clinical programs</li> </ul></div> </div> <div class="card-bottom-link"> <div> <a href="/p/cell-culture/gmp-human-t-cell-media_ccm038-gmp" > View GMP T Cell Media </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-3-media-to-text paragraph--view-mode--default mb-4 p-3"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--25-75"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_indv_media"> <div> <a href="https://www.bio-techne.com/research-areas/immune-cell-therapy/scaleready" title="Learn More About ScaleReady" target="_self" > <img src="https://resources.bio-techne.com/bio-techne-assets/images/products/g-rex-cytokines-410x410-pink-fill.png" alt="G-Rex and GMP cytokines for transition to clinical manufacturing" loading="lazy" > </a> </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_lg_sngl_frmtd_text_global"> <div><h3> </h3><h3><strong>ScaleReady™ Immune Cell Manufacturing Workflow</strong></h3><p>Our ScaleReady partnership accelerates immune cell therapy manufacturing, enabling high throughput parallel processing within a small footprint – efficiently producing more with less.</p><p> </p></div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_call_to_action_global"> <a href="/research-areas/immune-cell-therapy/scaleready" class="btn btn-primary" type="link"> Learn More About ScaleReady </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="iPSC Expansion Media"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2>Pluripotent Stem Cell Expansion Media</h2> <p>Achieve robust expansion and extended maintenance of your stem cells in well-defined culture media. ExCellerate iPSC Expansion Media sustains cell viability, genotype, and multi-lineage differentiation potential/pluripotency over long term culture and is compatible with various matrices and cell lines. Seamlessly transition to clinical applications with equivalent preclinical RUO and GMP-grade media.</p></div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h3>ExCellerate GMP and Preclinical RUO Expansion Media</h3></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><ul> <li>Animal component-free, all-in-one formulation</li> <li>Compatible with a variety of matrices and cell lines</li> <li>Maintains stable cell integrity over long-term culture</li> <li>GMP media manufactured in an animal-free facility with ISO:9001 and ISO:13485 certifications</li> <li>Media samples available — <strong><a href="/promotions/excellerate-ipsc-medium-sample">request yours today!</a></strong></li> </ul></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/products/gmp-excellerate-ipsc-expansion-media-525x315.png" alt="GMP ExCellerate iPSC Expansion Media" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--button-row paragraph--view-mode--default"> <div class="row"> <div class="col"> <div class="list-grid-wrap"> <div class="row"> <div class="col-md-4"> <a href="/p/cell-culture/excellerate-ipsc-expansion-medium-animal-free-gmp_ccm036-gmp" class="btn btn-primary" type="link"> View GMP iPSC Media </a> </div> <div class="col-md-4"> <a href="/p/cell-culture/excellerate-ipsc-expansion-medium-animal-component-free_ccm036" class="btn btn-primary" type="link"> View RUO iPSC Media </a> </div> <div class="col-md-4"> <a href="#request-form" class="btn btn-primary" type="link"> Request a Sample </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h4>View ExCellerate iPSC Expansion Media Data</h4></div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-ipsc-growth-comparison-with-ruo-and-gmp-media1" role="button" aria-expanded="false" aria-controls="expPara"> <h5>iPSC Growth Comparison with RUO and GMP Media</h5> </a> </div> <div class="collapse" id="expPara-ipsc-growth-comparison-with-ruo-and-gmp-media1" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/ipsc-growth-comparison-ruo-gmp-media-graph.png" alt="iPSC Growth Comparison with RUO and GMP Media Graph" loading="lazy" > </div> <br/> <div><p>Human iPSCs expanded in either the RUO or GMP version of ExCellerate iPSC Expansion Medium show similar growth kinetics. Catalog # <a href="/p/cell-culture/excellerate-ipsc-expansion-medium-animal-component-free_ccm036">CCM036</a> (RUO version) and Catalog # <a href="/p/cell-culture/excellerate-ipsc-expansion-medium-animal-free-gmp_ccm036-gmp">CCM036-GMP</a> have the same formulation and are manufactured with the same high quality animal-free components. No significant difference in growth kinetics is seen when culturing iPSC in either CCM036 (light blue) or CCM036-GMP (blue).</p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-ipsc-differentiation-after-expansion-in-excellerate-ipsc-expansion-medium14" role="button" aria-expanded="false" aria-controls="expPara"> <h5>iPSC Differentiation after Expansion in ExCellerate iPSC Expansion Medium</h5> </a> </div> <div class="collapse" id="expPara-ipsc-differentiation-after-expansion-in-excellerate-ipsc-expansion-medium14" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/ipsc-expansion-and-differentiation-900x220.png" alt="Representative images of iPSC Expansion and Differentiation" loading="lazy" > </div> <br/> <div><p>Representative images of iPSC expansion and differentiation. Image A shows iPSCs expanded in culture using ExCellerate iPSC Expansion Medium (Catalog # <a href="/p/cell-culture/excellerate-ipsc-expansion-medium-animal-component-free_ccm036-GMP">CCM036-GMP</a>). Image B shows the differentiation into PDX-1 expressing pancreatic progenitors (<a href="/p/antibodies/human-pdx-1-ipf1-antibody_af2419">AF2419</a>). Image C shows the presence of large population of C-peptide (<a href="/p/antibodies/human-c-peptide-antibody-790904_mab14171">MAB14171</a>) expressing monohormonal beta cells. Image D shows the co-expression of PDX-1 and C-peptide in the population.</p></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="thin-banner banner_wrapper"> <div class="banner-dark"> <div> <div><h2>Learn About Animal-Free Culture</h2></div> <a href="/reagents/cell-culture-reagents/serum-free-animal-free" > <button type="button" class="btn btn-primary"> Culture Your Cells Serum-Free and Animal-Free </button> </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-1-text-to-media mb-4 paragraph--view-mode--default p-3"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--75-25"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_header_2_global"> <div><h3>Transitioning Research to GMP</h3></div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>As you advance from discovery into process development and clinical manufacturing, we make the transition as efficient and seamless as possible. Our GMP materials are based on our research-grade products wherever possible. If GMP-grade materials are not available, our Custom Services team will work with you to convert RUO materials into products appropriate for manufacturing use.</p></div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_call_to_action_global"> <a href="/gmp-products/ruo-to-gmp" class="btn btn-primary" type="link"> Streamline Your Transition to GMP </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_indv_media"> <div> <a href="https://www.bio-techne.com/gmp-products/ruo-to-gmp" title="Streamline Your Transition to GMP" > <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/gmp-materials-facility1050x700.png" alt="GMP Facility Materials" loading="lazy" > </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>ExCellerate Cell Expansion Media Resources</h2> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="https://resources.bio-techne.com/bio-techne-assets/images/literature/bio-techne-til-expansion-with-t-cell-media-v2.pdf" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/literature/an-til-expansion-t-cell-media-thumbnail.png" alt="Application Note: Enhance Your TIL Expansion with ExCellerate T Cell Media" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="https://resources.bio-techne.com/bio-techne-assets/images/literature/bio-techne-til-expansion-with-t-cell-media-v2.pdf" > <h3 class="card_title_htag"> <div>Enhance Your TIL Expansion with ExCellerate T Cell Media</div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="https://resources.bio-techne.com/bio-techne-assets/images/literature/bio-techne-til-expansion-with-t-cell-media-v2.pdf" > Read Application Note </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="https://resources.bio-techne.com/bio-techne-assets/images/pdfs/an-ipsc-morphology-differentiation.pdf" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/literature/an-ipsc-morphology-differentiation-thumbnail.png" alt="Application Note: iPSC Colony Morphology Matters" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="https://resources.bio-techne.com/bio-techne-assets/images/pdfs/an-ipsc-morphology-differentiation.pdf" > <h3 class="card_title_htag"> <div>iPSC Colony Morphology Matters</div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="https://resources.bio-techne.com/bio-techne-assets/images/pdfs/an-ipsc-morphology-differentiation.pdf" > Read Application Note </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/organoid-handbook" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/literature/organoid-culture-handbook-650x500.png" alt="Cover of the Organoid handbook by Bio-Techne." loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/literature/organoid-handbook" > <h3 class="card_title_htag"> <div>The Organoid Handbook</div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/organoid-handbook" > Download Handbook </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2><a name="request-form"></a>Request an ExCellerate Cell Expansion Media Sample</h2> <p>Test ExCellerate Cell Expansion Media with your cells. Fill out the form below to request a sample.</p></div> </div> </div> <div> <div id="block-bio-techne-global-content" class="paragraph"> <article> <div class="row"> <div class="col-8 offset-md-2 offset-sm-0"> <script src="//info.bio-techne.com/js/forms2/js/forms2.min.js"></script> <form id="mktoForm_11622"></form> <script>MktoForms2.loadForm("//info.bio-techne.com", "584-XSF-168", 11622);</script> </div> </div> </article> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h3>Common ExCellerate Cell Expansion Media Questions</h3></div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-what-is-excellerate-cell-expansion-media5" role="button" aria-expanded="false" aria-controls="expPara"> <h5>What is ExCellerate Cell Expansion Media?</h5> </a> </div> <div class="collapse" id="expPara-what-is-excellerate-cell-expansion-media5" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>ExCellerate media are formulated and optimized for the <em>ex vivo</em> culture of immune cells and stem cells. Unlike traditional serum-containing culture media, these media provide a stable culture and optimized environment, devoid of non-human animal-derived products. ExCellerate iPSC medium does not contain any animal-derived or human-derived products. ExCellerate media support the expansion of specific cell types (T cells, NK cells, B cells, or pluripotent stem cells).</p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-how-do-you-define-xeno-free-and-animal-component-free-culture-media15" role="button" aria-expanded="false" aria-controls="expPara"> <h5>How do you define xeno-free and animal component-free culture media?</h5> </a> </div> <div class="collapse" id="expPara-how-do-you-define-xeno-free-and-animal-component-free-culture-media15" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>Xeno-free media do not contain any non-human animal-derived components. Xeno-free media may contain human-derived components such as serum, holo-transferrin, or insulin. Animal component-free media do not contain any animal- or human-derived components. Elimination of these components further improves culture consistency compared to xeno-free media.</p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-what-is-the-benefit-of-serum-free-cell-culture7" role="button" aria-expanded="false" aria-controls="expPara"> <h5>What is the benefit of serum-free cell culture?</h5> </a> </div> <div class="collapse" id="expPara-what-is-the-benefit-of-serum-free-cell-culture7" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>Using serum-free media eliminates variability from trace animal components in your culture media and reduces the risk of inconsistent results. Cultures expanded in these media are unaffected by the presence of unknown levels of vitamins, hormones, and growth factors in serum.</p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-what-are-organoids25" role="button" aria-expanded="false" aria-controls="expPara"> <h5>What are organoids?</h5> </a> </div> <div class="collapse" id="expPara-what-are-organoids25" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>An organoid is a miniaturized version of an organ that can be generated in vitro under specific culture conditions from primary tissue, embryonic stem cells (ESCs), or induced pluripotent stem cells (iPSCs). Organoids have similar cellular composition and architecture as their tissues of origin and exhibit functional similarities. They are typically composed of multiple different cell types, which are properly arranged with respect to both each other and the extracellular matrix. Organoids are not only capable of self-organization, they are also capable of self-renewal, and are amenable to long-term cultivation and manipulation. Researchers have developed organoid systems for many different human tissues, including intestine, stomach, colon, liver, lung, heart, pancreas, kidney, prostate, inner ear, brain, and heart, among others. These 3D <em>in vitro</em> model systems have opened new avenues for investigating organ development, studying physiology and disease, screening drugs, and exploring personalized medicine.</p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-what-are-the-different-kinds-of-stem-cells23" role="button" aria-expanded="false" aria-controls="expPara"> <h5>What are the different kinds of stem cells?</h5> </a> </div> <div class="collapse" id="expPara-what-are-the-different-kinds-of-stem-cells23" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>Different types of stem cells are identifiable by their specification potential.</p> <ul> <li>Pluripotent stem cells - generate all the embryonic germ layers (e.g. endoderm, ectoderm and mesoderm), but are unable to generate extra-embryonic tissues (e.g. placenta). Best exemplified by embryonic stem cells (ESCs) which are derived from the inner cell mass of the blastocyst. Induced pluripotent stem cells (iPSCs) are reprogrammed from adult differentiated cells (e.g. fibroblasts, peripheral blood mononuclear cells).</li> <li>Totipotent stem cells - generate all the cell types in the organism (e.g. zygote or fertilized egg).</li> <li>Multipotent stem cells - generate a limited number of cell types based on their tissue of origin.</li> <li>Oligopotent stem cells - generate few cell types which are closely related (e.g. myeloid stem cells).</li> <li>Unipotent stem cells - generate one cell type (e.g. epidermal stem cells, muscle stem cells).</li> <li>Mesenchymal stem cells - give rise to fat, bone, muscle and cartilage.</li> <li>Hematopoietic stem cells - give rise to different types of blood cells (e.g. platelets, red and white cells).</li> <li>Neural stem cells - give rise to neurons, oligodendrocytes and astrocytes.</li> </ul></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h4>Additional Resources</h4></div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-website-resources25" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Website Resources</h5> </a> </div> <div class="collapse" id="expPara-website-resources25" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><ul><li><a href="/gmp-products/ruo-to-gmp">Transitioning to Animal-Free and GMP</a></li><li><a href="/gmp-products/gmp-capabilities">GMP Capabilities</a></li><li><a href="/reagents/cell-culture-reagents/serum-free-animal-free">Serum-Free and Animal-Free Cell Culture</a></li><li><a href="/research-areas/organoids-3d-culture">Organoids and 3D Culture</a></li><li><a href="/reagents/cell-culture-reagents/cultrex-bme-and-ecm-proteins">Cultrex™ Basement Membrane Extracts and Extracellular Matrix Proteins</a></li><li><a href="/research-areas/stem-cells">Stem Cells</a></li><li><a href="/research-areas/immune-cell-therapy/scaleready">G-Rex® Bioreactors</a></li><li><a href="/research-areas/immunology/immune-cell-selection-and-culture">Immune Cell Culture</a></li><li><a href="/reagents/proteins/fluorokines-fluorescent-labeled-proteins">Fluorokines™ Fluorescent-Labeled Recombinant Proteins</a></li><li><a href="/services/gene-engineering-services-tcbuster">TcBuster™ Gene Transfer System</a></li><li><a href="/research-areas/cell-and-gene-therapy">Cell and Gene Therapy</a></li></ul></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-literature20" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Literature</h5> </a> </div> <div class="collapse" id="expPara-literature20" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><ul> <li><a href="/resources/literature/organoid-handbook" target="_blank">The Organoid Culture Handbook</a></li> <li><a href="/resources/protocols-troubleshooting?tid_4%5B1006%5D=1006" target="_blank">Organoid Culture Protocols</a></li> <li><a href="https://www.regmednet.com/technology-digest-the-importance-of-serum-and-animal-component-free-media-for-stem-cell-based-therapeutics/" target="_blank">The Importance of Serum- and Animal-Free Media for Stem Cell-Based Therapeutics Article</a> at RegMedNet</li> <li><a href="https://www.rndsystems.com/protocol-types/stem-cell-protocols" target="_blank">Stem Cell Protocols</a> at R&D Systems</li> <li><a href="/resources/literature/t-cell-therapies-ebook" target="_blank">T Cell-Based Therapies eBook</a></li> <li><a href="https://www.rndsystems.com/resources/ebook/evolution-model-systems-toxicology-drug-screening-and-disease-modeling" target="_blank">Evolution of Cell Culture Model Systems eBook</a> at R&D Systems</li> <li><a href="https://www.rndsystems.com/protocol-types/immunology" target="_blank">Immune Cell Protocols</a> at R&D Systems</li> <li><a href="https://resources.rndsystems.com/pdfs/brochures/rndsystems-immunecell-isolation-brochure.pdf" target="_blank">Immune Cell Isolation & Culture Brochure</a> at R&D Systems</li> </ul></div> </div> </div> </div> </div> </div> </div> </article> </div> </div> </section> </main> </div> <div id="brandsbar-footer" class="bg-bt-primary"> <div class="container-wide container-fluid text-white"> <div class="row py-5"> <div class="footer-logo col-xl-3 col-lg-auto d-flex"> <img class="brand-bar-footer-logo" src="/themes/custom/bio_techne_global/logo.svg" alt="Bio-techne logo"> </div> <div class="col-xl-9 col-lg-auto"> <p class="ml-xl-4 bt-px_groteskbold 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