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Fleet Science Center is a cardboard cutout of Jon Chesnut, Senior Director of R&D for Synthetic Biology and Sample Prep. A mainstay of Southern California’s “biotech beach,” Chesnut credits his undergrad years at UCSD as the genesis of a dynamic career in the life sciences. “I" /> <meta property="og:url" content="https://www.thermofisher.com/blog/behindthebench/straight-from-the-scientist-jon-chesnut-on-crispr-versus-talen/" /> <meta property="og:site_name" content="Behind the Bench" /> <meta property="article:publisher" content="https://www.facebook.com/thermofisher" /> <meta property="article:published_time" content="2017-03-30T21:35:11+00:00" /> <meta property="article:modified_time" content="2017-03-31T17:21:43+00:00" /> <meta property="og:image" content="https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2017/03/crispr20dna20blog20hero201600x35020_8_-1.png" /> <meta property="og:image:width" content="1600" /> <meta property="og:image:height" content="350" /> <meta property="og:image:type" content="image/png" /> <meta name="author" content="Grace Yco" /> <meta name="twitter:card" content="summary_large_image" /> <meta name="twitter:creator" content="@thermofisher" /> <meta name="twitter:site" content="@thermofisher" /> <meta name="twitter:label1" content="Written by" /> <meta name="twitter:data1" content="Grace Yco" /> <meta name="twitter:label2" content="Est. reading time" /> <meta name="twitter:data2" content="5 minutes" /> <script type="application/ld+json" class="yoast-schema-graph">{"@context":"https://schema.org","@graph":[{"@type":"WebPage","@id":"https://www.thermofisher.com/blog/behindthebench/straight-from-the-scientist-jon-chesnut-on-crispr-versus-talen/","url":"https://www.thermofisher.com/blog/behindthebench/straight-from-the-scientist-jon-chesnut-on-crispr-versus-talen/","name":"Straight From The Scientist: Jon Chesnut on CRISPR versus TALEN - Behind the Bench","isPartOf":{"@id":"https://www.thermofisher.com/blog/behindthebench/#website"},"primaryImageOfPage":{"@id":"https://www.thermofisher.com/blog/behindthebench/straight-from-the-scientist-jon-chesnut-on-crispr-versus-talen/#primaryimage"},"image":{"@id":"https://www.thermofisher.com/blog/behindthebench/straight-from-the-scientist-jon-chesnut-on-crispr-versus-talen/#primaryimage"},"thumbnailUrl":"https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2017/03/crispr20dna20blog20hero201600x35020_8_-1.png","datePublished":"2017-03-30T21:35:11+00:00","dateModified":"2017-03-31T17:21:43+00:00","author":{"@id":"https://www.thermofisher.com/blog/behindthebench/#/schema/person/dccad2f709bc0af3d63bbed6a240122f"},"breadcrumb":{"@id":"https://www.thermofisher.com/blog/behindthebench/straight-from-the-scientist-jon-chesnut-on-crispr-versus-talen/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https://www.thermofisher.com/blog/behindthebench/straight-from-the-scientist-jon-chesnut-on-crispr-versus-talen/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https://www.thermofisher.com/blog/behindthebench/straight-from-the-scientist-jon-chesnut-on-crispr-versus-talen/#primaryimage","url":"https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2017/03/crispr20dna20blog20hero201600x35020_8_-1.png","contentUrl":"https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2017/03/crispr20dna20blog20hero201600x35020_8_-1.png","width":1600,"height":350},{"@type":"BreadcrumbList","@id":"https://www.thermofisher.com/blog/behindthebench/straight-from-the-scientist-jon-chesnut-on-crispr-versus-talen/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https://www.thermofisher.com/blog/behindthebench/"},{"@type":"ListItem","position":2,"name":"Straight From The Scientist: Jon Chesnut on CRISPR versus TALEN"}]},{"@type":"WebSite","@id":"https://www.thermofisher.com/blog/behindthebench/#website","url":"https://www.thermofisher.com/blog/behindthebench/","name":"Behind the Bench","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https://www.thermofisher.com/blog/behindthebench/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https://www.thermofisher.com/blog/behindthebench/#/schema/person/dccad2f709bc0af3d63bbed6a240122f","name":"Grace Yco","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https://www.thermofisher.com/blog/behindthebench/#/schema/person/image/","url":"https://secure.gravatar.com/avatar/909b491ecb5337b904e42d5f09cb089b?s=96&d=mm&r=g","contentUrl":"https://secure.gravatar.com/avatar/909b491ecb5337b904e42d5f09cb089b?s=96&d=mm&r=g","caption":"Grace Yco"},"description":"Grace has a bachelor's in biology from the University of San Diego. 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class="site-inner"><div class="content-sidebar-wrap"><main class="content"><article class="post-10346 post type-post status-publish format-standard has-post-thumbnail category-developmental-biology category-immunology tag-gene-editing entry" aria-label="Straight From The Scientist: Jon Chesnut on CRISPR versus TALEN"><header class="entry-header"><h1 class="entry-title">Straight From The Scientist: Jon Chesnut on CRISPR versus TALEN</h1> <span class="post-info-author"> By <a href="https://www.thermofisher.com/blog/author/grace-yco/" title="Posts by Grace Yco" rel="author">Grace Yco</a> </span> <span class="post-info-date"> 03.30.2017 </span> </header><div class="entry-content"><p><img decoding="async" src="https://www.thermofisher.com/blog/wp-content/uploads/sites/9/2017/03/crispr20dna20blog20hero201600x35020_8_-1.png" /></p> <p><em>Tucked within an exhibit in San Diego’s Reuben H. Fleet Science Center is a cardboard cutout of Jon Chesnut, Senior Director of R&D for Synthetic Biology and Sample Prep. A mainstay of Southern California’s “biotech beach,” Chesnut credits his undergrad years at UCSD as the genesis of a dynamic career in the life sciences. “I always liked science and tinkering with things,” he enthuses. Following grad school and a post-doctoral fellowship, Chesnut landed at Invitrogen in 1995. He spent his early days conducting molecular biology research and development and eventually led teams developing innovative cloning technologies, such as </em><a href="http://www.thermofisher.com/us/en/home/life-science/cloning/topo.html?cid=bid_SB_GES_StraightFromSciJC_blog_20170330topo" target="_blank"><em>TOPO PCR Cloning</em></a><em> and </em><a href="http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html?cid=bid_SB_GES_StraightFromSciJC_blog_20170330gateway" target="_blank"><em>Gateway Cloning</em></a><em>. Afterwards, stepping into the gene editing arena was a natural undertaking. On genome editing tools such as </em><a href="http://www.thermofisher.com/us/en/home/life-science/genome-editing/genome-editing-learning-center/designer-talen-technology-information.html?cid=bid_SB_GES_StraightFromSciJC_blog_20170330talen" target="_blank"><em>TALEN</em></a><em> and </em><a href="http://www.thermofisher.com/us/en/home/life-science/genome-editing/genome-editing-learning-center/crispr-cas9-technology-information.html?cid=bid_SB_GES_StraightFromSciJC_blog_20170330crispr" target="_blank"><em>CRISPR</em></a><em>, Chesnut now provides thought leadership pertinent to the dialogue surrounding an industry breakthrough topic. Fresh from a synbio media blitz, we catch up with Chesnut and get him to elaborate on the multi-faceted value of “engineering a cell to allow it do something it normally doesn’t.” Join us as we hear straight from the scientist:</em></p> <p><strong>Q: </strong><strong>Last year, you wrote a couple articles on CRISPR versus TALEN. Is TALEN getting phased out?</strong></p> <p><strong>A: </strong>No, in fact we have a program to relaunch TALENs as a <a href="http://www.thermofisher.com/us/en/home/life-science/genome-editing.html?cid=bid_SB_GES_StraightFromSciJC_blog_20170330getools" target="_blank">genome editing tool</a> which could be used just like <a href="http://www.thermofisher.com/us/en/home/life-science/genome-editing/genome-editing-learning-center/crispr-cas9-technology-information.html?cid=bid_SB_GES_StraightFromSciJC_blog_20170330crispr" target="_blank">CRISPR</a>. We can now easily make <a href="http://www.thermofisher.com/us/en/home/life-science/genome-editing/genome-editing-learning-center/designer-talen-technology-information.html?cid=bid_SB_GES_StraightFromSciJC_blog_20170330talen" target="_blank">TALENs</a> that perform comparably or better than CRISPR and are focusing on using them to edit parts of the genome where CRISPR is either inefficient or completely non-functional.</p> <div style="width: 410px" class="wp-caption alignright"><img decoding="async" class="alignright" style="float: right;margin-left: 5px;margin-right: 5px" src="https://www.thermofisher.com/blog/wp-content/uploads/sites/9/2017/03/jon20chesnut20thermo20fisher20scientific-1.png" width="400" height="250" /><p class="wp-caption-text">Jon Chesnut, Senior Director, R&D Synthetic Biology and Sample Prep</p></div> <p><strong>Q: </strong><strong>What’s the first step a researcher should take when it comes to determining which gene editing tool to use?</strong></p> <p>A: Technically, for gene knock outs, one could choose either tool. Overall, <a href="http://www.thermofisher.com/us/en/home/life-science/genome-editing/genome-editing-learning-center/crispr-cas9-technology-information.html?cid=bid_SB_GES_StraightFromSciJC_blog_20170330crispr" target="_blank">CRISPR</a> may have slightly higher cutting efficiency than <a href="http://www.thermofisher.com/us/en/home/life-science/genome-editing/genome-editing-learning-center/designer-talen-technology-information.html?cid=bid_SB_GES_StraightFromSciJC_blog_20170330talen" target="_blank">TALENs</a> and it’s efficient to make arrays of CRISPR guide RNAs that allow library-type screening. TALENs also can do a fine job but it’s not quite as straight forward to make a large array of TALEN pairs. If a researcher wants to do an edit, where there will be a repair of a mutation or insertion of DNA at a specific locus in a gene, TALENs may be a good bet. CRISPR targeting is dependent on the availability of a short ‘PAM’ sequence which can limit their utility for editing. If your target locus is more than 8-10 bases away from the nearest PAM, we’re finding that TALENS (which can be directed anywhere in the genome) are often the best tool. Also, if a researcher is interested in using their genome editing workflow in a commercial enterprise, Thermo Fisher Scientific can provide a clear path, whereas the CRISPR IP landscape is not clear yet.</p> <p><strong>Q: </strong><strong>Where do you see the future of genome editing going?</strong></p> <p>A: I think we can separate the future of genome editing into three areas:</p> <p>1) <strong>Cellular and gene therapy.</strong> A lot has been written about engineering cells to treat diseases like sickle cell anemia and even HIV using various gene editing approaches. Now we’re seeing immunotherapy applications, like CAR T-cell approaches, being used to successfully treat some cancers. These approaches rely on engineering cells with various tools, including CRISPR and TALENs.</p> <p>2) <strong>Cell and animal model development.</strong> Many of our customers are interested in creating new cell lines and animals (like mice and zebra fish) that model diseases, like cancer, for use in drug screening and development. We anticipate significant growth in <a href="http://www.thermofisher.com/us/en/home/life-science/genome-editing/genome-engineering-services/cell-line-engineering-services.html?cid=bid_SB_GES_StraightFromSciJC_blog_20170330celllines" target="_blank">engineered cell lines</a>, specifically in pharma, for use as more physiologically relevant models for disease. Lowering the bar for creation of models will lead the way to more relevant and complex functional genomics assay systems which promise to give the researcher a clearer insight into the mechanisms of disease. This, in turn, should allow the development of better therapies and cures.</p> <p>3) <strong>Agriculture.</strong> Both <a href="http://bit.ly/2nEUbep" target="_blank">TALENs</a> and <a href="http://www.thermofisher.com/us/en/home/life-science/genome-editing/genome-editing-learning-center/crispr-cas9-technology-information.html?cid=bid_SB_GES_StraightFromSciJC_blog_20170330crispr" target="_blank">CRISPR</a> are being widely used to engineer plants to facilitate new trait development. This essentially speeds up the natural process of selective breeding in many cases. In the end the promise is that these new tools will foster the development of new, more robust crops that can be used to more efficiently feed and fuel the growing world population.</p> <div style="width: 360px" class="wp-caption alignleft"><img decoding="async" class="alignleft" style="float: left;margin-left: 5px;margin-right: 5px" src="https://www.thermofisher.com/blog/wp-content/uploads/sites/9/2017/03/img_2126-1.jpg" width="350" height="401" /><p class="wp-caption-text">Jon Chesnut’s children enjoying their father’s cardboard cutout at the stem cell exhibit in the Reuben H. Fleet Science Center, San Diego</p></div> <p><strong>Q: </strong><strong>Finally, our most burning question—do you <em>really</em> have a life-sized cardboard cutout at the Museum of Man?</strong></p> <p>A: That was a fun one. There were a couple years where I was a research fellow in the Corporate Research Lab (Life Tech days) where I was scouting new technologies and had a small research lab. Life Tech was asked to participate in a stem cell exhibit so I got to travel down there to help them design the technical part of the exhibit. I was on my last visit to review it just prior to the opening when the lead curator asked me to put on a lab coat for a short photo op. It took all of two minutes and resulted in the cardboard cutout of me in the ‘lab’ part of the exhibit. It’s been there for almost five years and I get continuous comments (and some good natured flak) about it. My kids really got a kick out of it!</p> <p>Interested in hearing more from Jon Chesnut? Check out these articles:<br /> <a href="http://www.genengnews.com/gen-exclusives/analyzing-talen-vs-crispr/77900759">Genetic Engineering and Biotechnology News (GEN): Analyzing TALEN vs CRISPR</a><br /> <a href="http://www.biosciencetechnology.com/article/2016/10/crispr-gene-editing-tool-rule-them-all-not-necessarily">Bioscience Technology: CRISPR: A Gene-Editing Tool to Rule Them All? Not Necessarily</a></p> <p>For more on CRISPR and TALEN, visit our new <a href="http://www.thermofisher.com/us/en/home/life-science/genome-editing/genome-editing-learning-center.html?cid=bid_SB_GES_StraightFromSciJC_blog_20170330gelc" target="_blank">Gene Editing Learning Center</a>.<br /> <em>For Research Use Only. Not for use in diagnostic procedures.</em></p> <p>Sources:<br /> <a href="http://www.thermofisher.com/us/en/home/life-science/genome-editing.html">http://www.thermofisher.com/us/en/home/life-science/genome-editing.html</a><br /> <a href="http://www.thermofisher.com/us/en/home/life-science/genome-editing/genome-editing-learning-center.html?cid=bid_SB_GES_StraightFromSciJC_blog_20170330gelc">http://www.thermofisher.com/us/en/home/life-science/genome-editing/genome-editing-learning-center.html</a></p> <aside class="mashsb-container mashsb-main "><div class="mashsb_above_buttons">Share this article </div><div class="mashsb-box"><div class="mashsb-count mash-large" style="float:left"><div class="counts mashsbcount">331</div><span class="mashsb-sharetext">SHARES</span></div><div class="mashsb-buttons"><a class="mashicon-facebook mash-large mashsb-noshadow" href="https://www.facebook.com/sharer.php?u=https%3A%2F%2Fwww.thermofisher.com%2Fblog%2Fbehindthebench%2Fstraight-from-the-scientist-jon-chesnut-on-crispr-versus-talen%2F" target="_top" rel="nofollow"><span class="icon"></span><span class="text">Facebook</span></a><a class="mashicon-linkedin mash-large mashsb-noshadow" href="https://www.linkedin.com/shareArticle?trk=Straight+From+The+Scientist%3A+Jon+Chesnut+on+CRISPR+versus+TALEN&url=https%3A%2F%2Fwww.thermofisher.com%2Fblog%2Fbehindthebench%2Fstraight-from-the-scientist-jon-chesnut-on-crispr-versus-talen%2F" target="_top" rel="nofollow"><span class="icon"></span><span class="text">Linkedin</span></a><a class="mashicon-twitter mash-large mashsb-noshadow" href="https://twitter.com/intent/tweet?text=Straight%20From%20The%20Scientist%3A%20Jon%20Chesnut%20on%20CRISPR%20versus%20TALEN&url=https://www.thermofisher.com/blog/behindthebench/?p=10346&via=thermofisher" target="_top" rel="nofollow"><span class="icon"></span><span class="text">Twitter</span></a><a class="mashicon-mail mash-large mashsb-noshadow" href="mailto:?subject=Check%20out%20this%20article%20from%20Accelerating%20Science&body=Check%20out%20this%20article%20I%20just%20read%3Ahttps%3A%2F%2Fwww.thermofisher.com%2Fblog%2Fbehindthebench%2Fstraight-from-the-scientist-jon-chesnut-on-crispr-versus-talen%2F" target="_top" rel="nofollow"><span class="icon"></span><span class="text">Mail</span></a><div class="onoffswitch2 mash-large mashsb-noshadow" style="display:none"></div></div> </div> <div style="clear:both"></div><div class="mashsb-toggle-container"></div></aside> </div></article><section class="author-box"><div class="author-avatar one-sixth first"><a href="https://www.thermofisher.com/blog/author/grace-yco/"><img src="" /></a></div><h4 class="author-box-title"><a href="https://www.thermofisher.com/blog/author/grace-yco/">Grace Yco</a></h4><div class="author-box-content" itemprop="description"><p>Grace has a bachelor's in biology from the University of San Diego. Though no longer researching at the bench, she routinely takes her penchant for science, adds an aliquot of creativity and incubates overnight for peer-resonating content.</p> </div></section><div class="entry-comments" id="comments"><h3>Comments</h3><ol class="comment-list"> <li class="comment even thread-even depth-1" id="comment-6553"> <article id="article-comment-6553"> <header class="comment-header"> <p class="comment-author"> <img alt='' src='httpss://secure.gravatar.com/avatar/26ccf9542a0f99ceb00f3b584d4be60c?s=48&d=mm&r=g' srcset='httpss://secure.gravatar.com/avatar/26ccf9542a0f99ceb00f3b584d4be60c?s=96&d=mm&r=g 2x' class='avatar avatar-48 photo' height='48' width='48' loading='lazy' decoding='async'/><span class="comment-author-name">Tammy Schuster</span> <span class="says">says</span> </p> <p class="comment-meta"><time class="comment-time"><a class="comment-time-link" href="https://www.thermofisher.com/blog/behindthebench/straight-from-the-scientist-jon-chesnut-on-crispr-versus-talen/#comment-6553">January 28, 2020 at 2:47 pm</a></time></p> </header> <div class="comment-content"> <p>My High School son (JR) is very interested in Genome Editing… and has a presentation due soon for the whole year’s grade. Needs to phone interview some in this flield. He’s a smart kid and REALLY needs someone to help him About a 15 min interview. Would you be that guy? </p> <p>Tammy Schuster 683-478-6777<br /> For Conrad Schuster</p> </div> <div class="comment-reply"><a rel="nofollow" class="comment-reply-link" href="#comment-6553" data-commentid="6553" data-postid="10346" data-belowelement="article-comment-6553" data-respondelement="respond" data-replyto="Reply to Tammy Schuster" aria-label="Reply to Tammy Schuster">Reply</a></div> </article> </li> </ol></div> <div id="respond" class="comment-respond"> <h3 id="reply-title" class="comment-reply-title">Leave a Reply <small><a rel="nofollow" id="cancel-comment-reply-link" href="/behindthebench/straight-from-the-scientist-jon-chesnut-on-crispr-versus-talen/#respond" style="display:none;">Cancel reply</a></small></h3><form action="https://www.thermofisher.com/blog/behindthebench/wp-comments-post.php" method="post" id="commentform" class="comment-form" novalidate><p class="comment-notes"><span id="email-notes">Your email address will not be published.</span> <span class="required-field-message">Required fields are marked <span class="required">*</span></span></p><p class="comment-form-comment"><label for="comment">Comment <span class="required">*</span></label> <textarea id="comment" name="comment" cols="45" rows="8" maxlength="65525" required></textarea></p><p class="comment-form-author"><label for="author">Name <span class="required">*</span></label> <input id="author" name="author" type="text" value="" size="30" maxlength="245" autocomplete="name" required /></p> <p class="comment-form-email"><label for="email">Email <span class="required">*</span></label> <input id="email" name="email" type="email" value="" size="30" maxlength="100" aria-describedby="email-notes" autocomplete="email" required /></p> <p class="comment-form-url"><label for="url">Website</label> <input id="url" name="url" type="url" value="" size="30" maxlength="200" autocomplete="url" /></p> <p class="form-submit"><input name="submit" type="submit" id="submit" class="submit" value="Post Comment" /> <input type='hidden' name='comment_post_ID' value='10346' id='comment_post_ID' /> <input type='hidden' name='comment_parent' id='comment_parent' value='0' /> </p><p style="display: none;"><input type="hidden" id="akismet_comment_nonce" name="akismet_comment_nonce" value="0db1601aa4" /></p><p style="display: none !important;" class="akismet-fields-container" data-prefix="ak_"><label>Δ<textarea name="ak_hp_textarea" cols="45" rows="8" maxlength="100"></textarea></label><input type="hidden" id="ak_js_2" name="ak_js" value="218"/><script>document.getElementById( "ak_js_2" ).setAttribute( "value", ( new Date() ).getTime() );</script></p></form> </div> <div class="adjacent-entry-pagination pagination"><div class="pagination-previous alignleft"><i class="fa fa-icon fa-chevron-left"></i> <a href="https://www.thermofisher.com/blog/behindthebench/complete-guide-to-ion-ampliseq-technology-how-does-ion-ampliseq-technology-work/" rel="prev">Complete Guide to Ion Ampliseq Technology – How Does Ion Ampliseq Technology Work?</a></div><div class="pagination-next alignright"><a href="https://www.thermofisher.com/blog/behindthebench/increasing-security-solving-crime-join-us-at-hids-in-vienna-may-16-17/" rel="next">Increasing Security, Solving Crime – Join us at HIDS 2017 in Vienna</a> <i class="fa fa-icon fa-chevron-right"></i></div></div></main></div></div><footer class="site-footer"><div class="wrap"><div class="creds"><p class="footer-links"><a href="https://corporate.thermofisher.com/en/footer/privacy-statement.html" target="_blank" rel="noopener noreferrer">Privacy Statement</a><a href="https://corporate.thermofisher.com/en/footer/terms-and-conditions.html" target="_blank" rel="noopener noreferrer">Terms & Conditions</a><a href="/blog/locations/">Locations</a><a href="/blog/sitemap/">Sitemap</a></p><p>© 2025 <a href="https://www.thermofisher.com">Thermo Fisher Scientific</a>. 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