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expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); 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max-width: 270px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsysimage_cfad .image-contain" + "," + "#cq-image-jsp-"+"1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsysimage_cfad .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div container-id="cq-colctrl-lt8" comp-id="B1"><div class="text parbase section"><p>Functional analysis of regulatory RNAs like microRNA (miRNA) can help decipher complex cellular processes in development and disease etiology.</p><p>Our suite of synthetic miRNA mimics and inhibitors are:</p><ul><li>Chemically modified for optimal performance</li><li>Pre-designed for thousands of microRNAs across a wide range of species in miRBase v.22</li><li>Complemented by a suite of miRNA tools for detection and analysis</li></ul><p><a href="#mirVana-mirna-reagents">Learn more about our advanced mirVana miRNA reagents</a></p></div></div></div></div><div style="clear:both"></div><div class="well-cmp well-default well-gray-lighter section"><div class="well well-gray-lighter well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="LTRawHTML section"><style type="text/css"> #recent-searches { visibility:hidden; height:0px; } #searchForm { width:100%; padding:0px; -moz-border-radius: 5px; -o-border-radius: 5px; -webkit-border-radius: 5px; border: 0px solid #ccc; border-radius: 5px; margin-bottom:8px; } .group:after { content: "."; } </style><h2> Search for Pre-designed miRNA Mimics and Inhibitors </h2><div class="clearfix" id="searchForm"><img class="magellan-search-loading" src="/order/genome-database/assets/core/img/loader.gif"/></div><script> jQuery('#searchForm').load('//' + environmentURL.BIOINFOSERVER + '/genome-database/embeddedSearchForm?searchFormId=mirvana-mimics-inhibitors'); </script></div></div></div></div></div></div><div class="LTRawHTML section"></div><div class="heading section"><h2 id="selecting"> Selecting a miRNA Mimic or Inhibitor </h2></div><div class="text parbase section"><p>We recommend mirVana <a href="?open=mimics-tab">mimics</a> and <a href="?open=inhibitors-tab">inhibitors</a> for anyone getting started with functional analysis of endogenous microRNA and looking for the latest advances in specificity and potency. Our Pre-miR and Anti-miR reagents remain relevant for ongoing studies that have benefited from these original product formats.<a id="table"></a></p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-1429146959"><tbody><tr><th> </th><th colspan="2" style="text-align: center;">miRNA mimics<br> for gain-of-function studies</th><th colspan="2" style="text-align: center;">miRNA inhibitors<br> for loss-of-function studies</th></tr><tr><td style="background-color: #eee;"> </td><td style="background-color: #eee; width: 20%;">mirVana Mimics<br> RECOMMENDED</td><td style="background-color: #eee; width: 20%;">Pre-miR Mimics</td><td style="background-color: #eee; width: 20%;">mirVana Inhibitors<br> RECOMMENDED</td><td style="background-color: #eee; width: 20%;">Anti-miR Inhibitors</td></tr><tr><td><b>Content database coverage</b></td><td>miRBase v.22</td><td>miRBase v.15</td><td>miRBase v.22</td><td>miRBase v.15</td></tr><tr><td><b>Available formats</b></td><td>Individual tubes, <a href="?open=libraries-tab">custom plated collections</a>, or whole genome library</td><td>Individual tubes</td><td>Individual tubes, <a href="?open=libraries-tab">custom plated collections</a>, or whole genome library</td><td>Individual tubes</td></tr><tr><td><b>Product summary</b></td><td><ul><li>Chemical modifications prevent passenger strand entry into RISC</li><li>Highly specific microRNA mimics</li></ul></td><td><ul><li>RNA duplexes mimic native microRNA</li></ul></td><td><ul><li>Patented design and chemical modifications</li><li>Potent, long-lasting microRNA inhibition</li></ul></td><td><ul><li>Single-strand inhibitors of endogenous microRNAs</li></ul></td></tr></tbody></table></div></div><div comp-order="bycol" class="parsys_column cq-colctrl-bordered-lt3"><div class="parsys_column cq-colctrl-bordered-lt3-c0"><div container-id="cq-colctrl-bordered-lt3" comp-id="A1"><div class="text parbase section"><h2 style="text-align: center;"><img src="/content/dam/LifeTech/global/life-sciences/Cell%20analysis/images/0620/Document-Lab-Considerations-RNA-icon.svg" alt="icon" width="32px"> Custom synthesis</h2><ul><li><a href="/order/custom-genomic-products/tools/mirna/">Order a custom miRNA mimic or inhibitor</a></li></ul></div></div></div><div class="parsys_column cq-colctrl-bordered-lt3-c1"><div container-id="cq-colctrl-bordered-lt3" comp-id="B1"><div class="text parbase section"><h2 style="text-align: center;"><img src="/content/dam/LifeTech/global/life-sciences/images/Plate%20Protein%20Analysis.png" alt="plate icon"> Libraries</h2><ul><li><a href="/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/mirna-libraries.html">mirVana Mimic and Inhibitor Libraries</a></li></ul></div></div></div><div class="parsys_column cq-colctrl-bordered-lt3-c2"><div container-id="cq-colctrl-bordered-lt3" comp-id="C1"><div class="text parbase section"><h2 style="text-align: center;"><img src="/content/dam/LifeTech/global/promotions/images/rna/sample-prep-333-icon.png" alt="sample prep icon" width="32px;"> Controls</h2><ul><li><a href="#additional-products">Additional products for miRNA research</a></li></ul></div></div></div></div><div style="clear:both"></div><div class="heading section"><h2 id="order-positive-negative-controls"> Order miRNA Mimic and Inhibitor positive and negative controls </h2></div><div class="accordion section"><div class="accordion" id="afaf45ac965e486c93441e8d1eca1836" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#afaf45ac965e486c93441e8d1eca1836" href="#mimicpncaccordion"><span class="caret"></span> Mimic positive and negative controls </a></div><div class="accordion-body collapse " id="mimicpncaccordion"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">4464058,4464059,4464060,4464061,4464062,4464063,4464064,4464065,AM17110,AM17111,AM17150,AM17120,AM17121</div><div id='ltajax-5498502368650193168'></div><script type="text/javascript">(function() {$('#ltajax-5498502368650193168').load('/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/accordion_ca4f/itemspar/accordionitem_193e/itemParsys/productlist_af25.ajax.html?skulist=');})(); </script></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#afaf45ac965e486c93441e8d1eca1836" href="#inhibitorpncaccordion"><span class="caret"></span> Inhibitor positive and negative controls </a></div><div class="accordion-body collapse " id="inhibitorpncaccordion"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">4464076,4464077,4464078,4464079,4464080,4464081,AM17010,4392431,AM17011,AM17012</div><div id='ltajax-7258156856716915366'></div><script type="text/javascript">(function() {$('#ltajax-7258156856716915366').load('/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/accordion_ca4f/itemspar/accordionitem_9a5b/itemParsys/productlist_61e5.ajax.html?skulist=');})(); </script></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#afaf45ac965e486c93441e8d1eca1836').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#afaf45ac965e486c93441e8d1eca1836').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div class="heading section"><h2 id="mirVana-mirna-reagents"> mirVana miRNA reagents for functional analysis </h2></div><div class="tabworkflowcontainer section"><div class="tabbable plain-tab"><ul class="nav nav-tabs" id="myTab"><li class="active"><a data-toggle="tab" href="#container-21a21">miRNA mimics</a></li><li><a data-toggle="tab" href="#container-21a22">miRNA inhibitors</a></li><li><a data-toggle="tab" href="#container-21a23">miRNA libraries</a></li></ul><div class="tab-content" id="myTabContent"><div id="container-21a21" class="tab-pane active"><div class="container-21a21 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Mimics with superior specificity </h2></div><div class="text parbase section"><p>mirVana miRNA mimics are chemically modified double-stranded RNA molecules designed to mimic endogenous microRNAs (miRNAs), resulting in down-regulation of target mRNA translation due to mRNA sequestration or degradation. Like native miRNAs, these mimics have two strands—the mature, or guide strand, which is functional and used by the Argonaute (Ago) protein to target mRNAs, and the passenger strand (formerly called the star strand), which may or may not have native targets of its own.</p><p>The chemical modifications in mirVana miRNA mimics inactivate the passenger strand to help ensure that the guide strand (representing the desired mature miRNA) is taken up by Ago to produce the miRNA effect (<b>Figure 1</b>). mirVana miRNA mimics are designed to produce maximum and consistent effects at concentrations as low as 0.3 nM and are available individually or as libraries.</p></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainercontainer-21a21tabtextimage_8056"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a21/tab/textimage_8056/image.img.full.high.png/1657631390092.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a21/tab/textimage_8056/image.img.full.high.png/1657631390092.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a21/tab/textimage_8056/image.img.320.high.png/1657631390092.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a21/tab/textimage_8056/image.img.480.high.png/1657631390092.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a21/tab/textimage_8056/image.img.320.medium.png/1657631390092.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a21/tab/textimage_8056/image.img.620.high.png/1657631390092.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a21/tab/textimage_8056/image.img.320.low.png/1657631390092.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a21/tab/textimage_8056/image.img.480.medium.png/1657631390092.png" alt='mirVana miRNA mimics demonstrate high specificity' title='mirVana miRNA mimics demonstrate high specificity'></picture></div><style> #cq-textimage-jsp-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainercontainer-21a21tabtextimage_8056 { max-width: 100%; } #cq-textimage-jsp-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainercontainer-21a21tabtextimage_8056 img { width: 100%; max-width: 260px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small class="muted"><b>Figure 1. mirVana miRNA mimics demonstrate high specificity.</b> HeLa cells were transfected with one of six different mirVana miRNA mimics at 3 nM concentration and a corresponding reporter plasmid. The fold change in reporter gene expression for each miRNA mimic strand was determined by measuring expression in the presence of mimic relative to that of a negative control (set at 1.0). Each plasmid has the reporter gene cloned both in forward orientation (Fwd) to measure activity of the miRNA mimic mature strand, and in reverse/complement orientation (Rev) to test activity of the passenger strand. For all six mimics, mature strand activity is high (reporter gene expression reduced 5- to 10-fold compared with negative control), and passenger strand activity is low or absent (similar to negative control).</small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div><div id="container-21a22" class="tab-pane"><div class="container-21a22 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Inhibitors with excellent potency </h2></div><div class="text parbase section"><p>mirVana miRNA inhibitors are chemically modified, single-stranded oligonucleotides with patented secondary structure designed to specifically bind to and inhibit endogenous miRNAs, resulting in up-regulation of target mRNA translation. Compared with competitors, these inhibitors have the highest-potency <i>in vitro</i> inhibition at the lowest miRNA inhibitor concentration (<b>Figure 2</b>). Like the mimics, the mirVana miRNA inhibitors are available individually or as libraries.</p></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainercontainer-21a22tabtextimage_cde"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_cde/image.img.full.high.png/1657631390145.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_cde/image.img.full.high.png/1657631390145.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_cde/image.img.320.high.png/1657631390145.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_cde/image.img.480.high.png/1657631390145.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_cde/image.img.320.medium.png/1657631390145.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_cde/image.img.620.high.png/1657631390145.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_cde/image.img.320.low.png/1657631390145.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_cde/image.img.480.medium.png/1657631390145.png" alt='mirVana miRNA inhibitors are more potent than those from leading competitors' title='mirVana miRNA inhibitors are more potent than those from leading competitors'></picture></div><style> #cq-textimage-jsp-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainercontainer-21a22tabtextimage_cde { max-width: 100%; } #cq-textimage-jsp-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainercontainer-21a22tabtextimage_cde img { width: 100%; max-width: 256px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small class="muted"><b>Figure 2. mirVana miRNA inhibitors are more potent than those from leading competitors.</b> mirVana miRNA inhibitors and two other commercially available let-7 inhibitors were each transfected into HeLa cells at 10 nM concentration using <a href="/order/catalog/product/13778100">Lipofectamine RNAiMAX reagent</a>. Twenty-four hours later, HMGA2 mRNA levels (a natural target of the let-7 microRNA) were measured by qRT-PCR using TaqMan assays.</small></p></div></div><div class="clearfix"></div></div><div class="text parbase section"><h2>Take advantage of <i><i>in vivo</i></i>–ready reagents</h2> mirVana miRNA mimics and inhibitors are compatible with <i>in vivo</i> applications and have been validated with Invivofectamine 2.0 Reagent for <i>in vivo</i> delivery ( <b>Figure 3</b>) to achieve effective target modulation. <i>in vivo</i>–ready mirVana miRNA mimics and inhibitors are HPLC-purified and <i>in vivo</i>-processed for salt-balancing, sterile filtration, and endotoxin testing to reduce potential immune responses and are ready for immediate <i>in vivo</i> use. </div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainercontainer-21a22tabtextimage_6a37"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_6a37/image.img.full.high.png/1657631390173.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_6a37/image.img.full.high.png/1657631390173.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_6a37/image.img.320.high.png/1657631390173.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_6a37/image.img.480.high.png/1657631390173.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_6a37/image.img.320.medium.png/1657631390173.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_6a37/image.img.620.high.png/1657631390173.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_6a37/image.img.320.low.png/1657631390173.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_6a37/image.img.480.medium.png/1657631390173.png" alt='mirVana miRNA inhibitors effectively suppress miRNA in vivo' title='mirVana miRNA inhibitors effectively suppress miRNA in vivo'></picture></div><style> #cq-textimage-jsp-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainercontainer-21a22tabtextimage_6a37 { max-width: 100%; } #cq-textimage-jsp-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainercontainer-21a22tabtextimage_6a37 img { width: 100%; max-width: 265px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small class="muted"><b>Figure 3. mirVana miRNA inhibitors effectively suppress miRNA <i>in vivo</i>.</b> miR-122 or Negative Control #1 mirVana miRNA inhibitors were complexed with Invivofectamine 2.0 reagent and injected into the tail veins of Balb-C mice on three consecutive days at 7 mg per kg body weight. Twenty-four hours after the last injection, expression levels of four natural targets of miR-122 were measured in the liver using TaqMan assays. Significant up-regulation of all four mRNAs was detected in mice treated with miR-122 inhibitor, as compared with mice that received no treatment or the negative control. Results indicate that mirVana miRNA inhibitors were efficiently delivered to the liver with Invivofectamine 2.0, where they inactivated miR-122, leading to up-regulation of genes naturally suppressed by miR-122.</small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div><div id="container-21a23" class="tab-pane"><div class="container-21a23 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> miRNA libraries for high-throughput studies (96-well or 384-well plates) </h2></div><div class="text parbase section"><p>In addition to our <a href="/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/mirna-libraries.html">pre-defined miRNA libraries</a>, mirVana mimics and inhibitors are available as custom collections.</p><ul><li>96- or 384-well plates, 384-well Echo-compatible plates</li><li>Minimum order of 20 reagents</li><li>Perfect for pilot studies, primary screen follow-up, or a customized functional genomics screen</li></ul><p><a href="/sg/en/home/global/forms/gene-editing-and-silencing-quote-request.html" class="cta-link">Request a quote for a custom miRNA library</a></p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--1350737192"><tbody><tr><th style="width:66%;">Product line</th><th>Quantity per well</th></tr><tr><td>mirVana miRNA mimics–human, mouse, or rat</td><td>0.25 or 1.0 nmol/well</td></tr><tr><td>mirVana miRNA inhibitors–human, mouse, or rat</td><td>0.25 or 1.0 nmol/well</td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div><div class="workflow-accordion accordion" id="accordion-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainer"><div class="accordion-group"><div class="accordion-heading active"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainer" href="#collapse1"><span class="caret"></span><strong> miRNA mimics </strong></a></div><div id="collapse1" class="accordion-body collapse in "><div class="accordion-inner container-21a21"><div class="container-21a21 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Mimics with superior specificity </h2></div><div class="text parbase section"><p>mirVana miRNA mimics are chemically modified double-stranded RNA molecules designed to mimic endogenous microRNAs (miRNAs), resulting in down-regulation of target mRNA translation due to mRNA sequestration or degradation. Like native miRNAs, these mimics have two strands—the mature, or guide strand, which is functional and used by the Argonaute (Ago) protein to target mRNAs, and the passenger strand (formerly called the star strand), which may or may not have native targets of its own.</p><p>The chemical modifications in mirVana miRNA mimics inactivate the passenger strand to help ensure that the guide strand (representing the desired mature miRNA) is taken up by Ago to produce the miRNA effect (<b>Figure 1</b>). mirVana miRNA mimics are designed to produce maximum and consistent effects at concentrations as low as 0.3 nM and are available individually or as libraries.</p></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainercontainer-21a21tabtextimage_8056"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a21/tab/textimage_8056/image.img.full.high.png/1657631390092.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a21/tab/textimage_8056/image.img.full.high.png/1657631390092.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a21/tab/textimage_8056/image.img.320.high.png/1657631390092.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a21/tab/textimage_8056/image.img.480.high.png/1657631390092.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a21/tab/textimage_8056/image.img.320.medium.png/1657631390092.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a21/tab/textimage_8056/image.img.620.high.png/1657631390092.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a21/tab/textimage_8056/image.img.320.low.png/1657631390092.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a21/tab/textimage_8056/image.img.480.medium.png/1657631390092.png" alt='mirVana miRNA mimics demonstrate high specificity' title='mirVana miRNA mimics demonstrate high specificity'></picture></div><style> #cq-textimage-jsp-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainercontainer-21a21tabtextimage_8056 { max-width: 100%; } #cq-textimage-jsp-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainercontainer-21a21tabtextimage_8056 img { width: 100%; max-width: 260px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small class="muted"><b>Figure 1. mirVana miRNA mimics demonstrate high specificity.</b> HeLa cells were transfected with one of six different mirVana miRNA mimics at 3 nM concentration and a corresponding reporter plasmid. The fold change in reporter gene expression for each miRNA mimic strand was determined by measuring expression in the presence of mimic relative to that of a negative control (set at 1.0). Each plasmid has the reporter gene cloned both in forward orientation (Fwd) to measure activity of the miRNA mimic mature strand, and in reverse/complement orientation (Rev) to test activity of the passenger strand. For all six mimics, mature strand activity is high (reporter gene expression reduced 5- to 10-fold compared with negative control), and passenger strand activity is low or absent (similar to negative control).</small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainer" href="#collapse2"><span class="caret"></span><strong> miRNA inhibitors </strong></a></div><div id="collapse2" class="accordion-body collapse "><div class="accordion-inner container-21a22"><div class="container-21a22 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Inhibitors with excellent potency </h2></div><div class="text parbase section"><p>mirVana miRNA inhibitors are chemically modified, single-stranded oligonucleotides with patented secondary structure designed to specifically bind to and inhibit endogenous miRNAs, resulting in up-regulation of target mRNA translation. Compared with competitors, these inhibitors have the highest-potency <i>in vitro</i> inhibition at the lowest miRNA inhibitor concentration (<b>Figure 2</b>). Like the mimics, the mirVana miRNA inhibitors are available individually or as libraries.</p></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainercontainer-21a22tabtextimage_cde"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_cde/image.img.full.high.png/1657631390145.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_cde/image.img.full.high.png/1657631390145.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_cde/image.img.320.high.png/1657631390145.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_cde/image.img.480.high.png/1657631390145.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_cde/image.img.320.medium.png/1657631390145.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_cde/image.img.620.high.png/1657631390145.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_cde/image.img.320.low.png/1657631390145.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_cde/image.img.480.medium.png/1657631390145.png" alt='mirVana miRNA inhibitors are more potent than those from leading competitors' title='mirVana miRNA inhibitors are more potent than those from leading competitors'></picture></div><style> #cq-textimage-jsp-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainercontainer-21a22tabtextimage_cde { max-width: 100%; } #cq-textimage-jsp-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainercontainer-21a22tabtextimage_cde img { width: 100%; max-width: 256px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small class="muted"><b>Figure 2. mirVana miRNA inhibitors are more potent than those from leading competitors.</b> mirVana miRNA inhibitors and two other commercially available let-7 inhibitors were each transfected into HeLa cells at 10 nM concentration using <a href="/order/catalog/product/13778100">Lipofectamine RNAiMAX reagent</a>. Twenty-four hours later, HMGA2 mRNA levels (a natural target of the let-7 microRNA) were measured by qRT-PCR using TaqMan assays.</small></p></div></div><div class="clearfix"></div></div><div class="text parbase section"><h2>Take advantage of <i><i>in vivo</i></i>–ready reagents</h2> mirVana miRNA mimics and inhibitors are compatible with <i>in vivo</i> applications and have been validated with Invivofectamine 2.0 Reagent for <i>in vivo</i> delivery ( <b>Figure 3</b>) to achieve effective target modulation. <i>in vivo</i>–ready mirVana miRNA mimics and inhibitors are HPLC-purified and <i>in vivo</i>-processed for salt-balancing, sterile filtration, and endotoxin testing to reduce potential immune responses and are ready for immediate <i>in vivo</i> use. </div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainercontainer-21a22tabtextimage_6a37"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_6a37/image.img.full.high.png/1657631390173.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_6a37/image.img.full.high.png/1657631390173.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_6a37/image.img.320.high.png/1657631390173.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_6a37/image.img.480.high.png/1657631390173.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_6a37/image.img.320.medium.png/1657631390173.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_6a37/image.img.620.high.png/1657631390173.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_6a37/image.img.320.low.png/1657631390173.png,https://www.thermofisher.com/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/jcr:content/MainParsys/tabworkflowcontainer/container-21a22/tab/textimage_6a37/image.img.480.medium.png/1657631390173.png" alt='mirVana miRNA inhibitors effectively suppress miRNA in vivo' title='mirVana miRNA inhibitors effectively suppress miRNA in vivo'></picture></div><style> #cq-textimage-jsp-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainercontainer-21a22tabtextimage_6a37 { max-width: 100%; } #cq-textimage-jsp-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainercontainer-21a22tabtextimage_6a37 img { width: 100%; max-width: 265px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small class="muted"><b>Figure 3. mirVana miRNA inhibitors effectively suppress miRNA <i>in vivo</i>.</b> miR-122 or Negative Control #1 mirVana miRNA inhibitors were complexed with Invivofectamine 2.0 reagent and injected into the tail veins of Balb-C mice on three consecutive days at 7 mg per kg body weight. Twenty-four hours after the last injection, expression levels of four natural targets of miR-122 were measured in the liver using TaqMan assays. Significant up-regulation of all four mRNAs was detected in mice treated with miR-122 inhibitor, as compared with mice that received no treatment or the negative control. Results indicate that mirVana miRNA inhibitors were efficiently delivered to the liver with Invivofectamine 2.0, where they inactivated miR-122, leading to up-regulation of genes naturally suppressed by miR-122.</small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-1b2f878a-e730-46ed-a61a-a005c600bb1fMainParsystabworkflowcontainer" href="#collapse3"><span class="caret"></span><strong> miRNA libraries </strong></a></div><div id="collapse3" class="accordion-body collapse "><div class="accordion-inner container-21a23"><div class="container-21a23 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> miRNA libraries for high-throughput studies (96-well or 384-well plates) </h2></div><div class="text parbase section"><p>In addition to our <a href="/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/mirna-libraries.html">pre-defined miRNA libraries</a>, mirVana mimics and inhibitors are available as custom collections.</p><ul><li>96- or 384-well plates, 384-well Echo-compatible plates</li><li>Minimum order of 20 reagents</li><li>Perfect for pilot studies, primary screen follow-up, or a customized functional genomics screen</li></ul><p><a href="/sg/en/home/global/forms/gene-editing-and-silencing-quote-request.html" class="cta-link">Request a quote for a custom miRNA library</a></p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--1017822588"><tbody><tr><th style="width:66%;">Product line</th><th>Quantity per well</th></tr><tr><td>mirVana miRNA mimics–human, mouse, or rat</td><td>0.25 or 1.0 nmol/well</td></tr><tr><td>mirVana miRNA inhibitors–human, mouse, or rat</td><td>0.25 or 1.0 nmol/well</td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div></div></div><div class="heading section"><h2 id="additional-products"><br>Additional products for miRNA research </h2></div><div class="text parbase section"><p>miRNAs require special tools for accurate and sensitive analysis. We offer a broad portfolio of products that provides complete solutions for either miRNA expression profiling or a targeted quantitation/target validation.</p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--905909526"><tbody><tr><th style="25%;text-align:center;">miRNA custom synthesis and libraries</th><th style="25%;text-align:center;"><a href="/sg/en/home/life-science/dna-rna-purification-analysis/rna-extraction/rna-types/micro-rna-and-small-rna-isolation.html">miRNA and small RNA extraction</a></th><th style="25%;text-align:center;"><a href="/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis.html">miRNA analysis solutions</a></th><th style="25%;text-align:center;"><a href="/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors.html">miRNA and non-coding RNA microarrays</a></th></tr><tr><td><ul><li><a href="/order/custom-genomic-products/tools/mirna/">Custom miRNA Mimics & Inhibitors</a></li><li><a href="/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/mirna-mimics-inhibitors/mirna-libraries.html">miRNA Mimic & Inhibitor Libraries</a></li></ul></td><td><ul><li><a href="/sg/en/home/life-science/dna-rna-purification-analysis/rna-extraction/rna-types/micro-rna-and-small-rna-isolation.html">MicroRNA Isolation Kits</a></li></ul></td><td><ul><li><a href="/sg/en/home/life-science/pcr/real-time-pcr/real-time-pcr-assays/mirna-ncrna-taqman-assays.html">TaqMan miRNA Assays</a></li><li><a href="/order/catalog/product/4391848">Cells-to-CT for miRNA</a></li><li><a href="/sg/en/home/life-science/epigenetics-noncoding-rna-research/mirna-analysis/quantigene-mirna-singleplex-assay.html">QuantiGene miRNA Singleplex Assay</a></li></ul></td><td><ul><li><a href="/sg/en/home/life-science/microarray-analysis/mirna-profiling-microarrays.html">miRNA Profiling with Microarrays</a></li></ul></td></tr></tbody></table></div></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="well-cmp"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3> Shop for everyday lab essentials to power your transfection steps </h3></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt2"><div class="parsys_column cq-colctrl-lt2-c0"><div container-id="cq-colctrl-lt2" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-2ceb2e9b-d054-467a-b4a5-6dd4cca43431MainParsyswell_ad6uiparimage_4060"><picture><source srcset='https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/pca-reference-components/jcr:content/MainParsys/well_ad6/uipar/image_4060/foregroundimg.img.320.low.jpg/1729526171660.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/pca-reference-components/jcr:content/MainParsys/well_ad6/uipar/image_4060/foregroundimg.img.320.medium.jpg/1729526171660.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/pca-reference-components/jcr:content/MainParsys/well_ad6/uipar/image_4060/foregroundimg.img.480.medium.jpg/1729526171660.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/pca-reference-components/jcr:content/MainParsys/well_ad6/uipar/image_4060/foregroundimg.img.620.high.jpg/1729526171660.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/pca-reference-components/jcr:content/MainParsys/well_ad6/uipar/image_4060/foregroundimg.img.full.high.jpg/1729526171660.jpg' media="(min-width: 1025px)"><img 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