CINXE.COM
Protocells and RNA Self-Replication
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Strict//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-strict.dtd"> <html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en"> <head> <meta http-equiv="Content-Type" content="text/html; charset=UTF-8" /> <title>Protocells and RNA Self-Replication </title> <meta name="googlebot" content="NOODP" /> <meta name="description" content="A new type of review journal, featuring comprehensive collections of expert review articles on important topics in the molecular life sciences" /> <meta content="/cshperspect/10/9/a034801.atom" name="HW.identifier" /> <meta name="DC.Format" content="text/html" /> <meta name="DC.Language" content="en" /> <meta content="Protocells and RNA Self-Replication" name="DC.Title" /> <meta content="10.1101/cshperspect.a034801" name="DC.Identifier" /> <meta content="2018-09-01" name="DC.Date" /> <meta content="Cold Spring Harbor Laboratory Press" name="DC.Publisher" /> <meta content="Gerald F. Joyce" name="DC.Contributor" /> <meta content="Jack W. Szostak" name="DC.Contributor" /> <meta content="052" name="citation_collection_id" /> <meta content="Cold Spring Harbor Perspectives in Biology" name="citation_journal_title" /> <meta content="Cold Spring Harb Perspect Biol" name="citation_journal_abbrev" /> <meta content="Cold Spring Harbor Lab" name="citation_publisher" /> <meta content="" name="citation_issn" /> <meta content="1943-0264" name="citation_issn" /> <meta name="citation_author" content="Gerald F. Joyce" /> <meta name="citation_author_orcid" content="0000-0003-0603-2874" /> <meta name="citation_author_institution" content="1The Salk Institute for Biological Studies, La Jolla, California 92037" /> <meta name="citation_author" content="Jack W. Szostak" /> <meta name="citation_author_institution" content="2Howard Hughes Medical Institute and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114" /> <meta content="Protocells and RNA Self-Replication" name="citation_title" /> <meta content="09/01/2018" name="citation_date" /> <meta content="10" name="citation_volume" /> <meta content="9" name="citation_issue" /> <meta content="a034801" name="citation_firstpage" /> <meta content="10/9/a034801" name="citation_id" /> <meta content="10/9/a034801" name="citation_id_from_sass_path" /> <meta content="cshperspect;10/9/a034801" name="citation_mjid" /> <meta content="10.1101/cshperspect.a034801" name="citation_doi" /> <meta content="http://cshperspectives.cshlp.org/content/10/9/a034801.abstract" name="citation_abstract_html_url" /> <meta content="http://cshperspectives.cshlp.org/content/10/9/a034801.full" name="citation_fulltext_html_url" /> <meta content="http://cshperspectives.cshlp.org/content/10/9/a034801.full.pdf" name="citation_pdf_url" /> <meta content="/content/10/9.cover.gif" name="issue_cover_image" /> <meta content="http://cshperspectives.cshlp.org/content/10/9/a034801" name="citation_public_url" /> <meta content="30181195" name="citation_pmid" /> <meta name="citation_section" content="Concept" /> <meta name="robots" content="noarchive" /> <link href="/content/10/9/a033613.short" rel="prev" /> <link href="/content/10/10/a028431.short" rel="next" /> <link rel="stylesheet" type="text/css" media="all" href="/shared/css/hw-global.css" /> <link rel="stylesheet" type="text/css" media="all" href="/shared/css/standard-designs/design2/main.css" /> <link rel="stylesheet" type="text/css" media="all" href="/publisher/css/hw-cshljnls-global.css" /> <link rel="stylesheet" type="text/css" media="all" href="/local/css/hw-journal-global.css" /> <link rel="stylesheet" type="text/css" media="all" href="/shared/css/hw-citation-2.0-hacks.css" /> <link rel="stylesheet" type="text/css" media="print" href="/shared/css/hw-print.css" /> <link rel="stylesheet" type="text/css" media="print" href="/publisher/css/hw-cshljnls-print.css" /> <link rel="stylesheet" type="text/css" media="all" href="/shared/css/hw-page-content.css" /> <link rel="stylesheet" type="text/css" media="all" href="/publisher/css/hw-cshljnls-page-content.css" /> <link rel="stylesheet" type="text/css" media="all" href="/local/css/hw-journal-page-content.css" /> <link rel="stylesheet" type="text/css" media="all" href="/site/misc/custom-global.css" /><script type="text/javascript" id="session-d990657e1">var callbackToken='51548FE03A107DA';</script><script type="text/javascript" src="/shared/js/jquery-min.js"></script><script type="text/javascript" src="/shared/js/fingerprint.js"></script><script type="text/javascript" src="/shared/js/hw-shared.js"></script><script type="text/javascript" src="/shared/js/design/hw-design1.js"></script><script type="text/javascript" src="/publisher/js/cshl-shared.js"></script><script type="text/javascript" src="/shared/js/pages/hw-content.js"></script><script type="text/javascript" src="/publisher/js/cshl-content.js"></script><script type="text/javascript" async="async" src="https://scholar.google.com/scholar_js/casa.js"></script><script type="text/javascript" src="/site/misc/custom-global.js"></script></head> <body> <div class="hw-gen-page pagetype-content" id="pageid-content" itemscope="itemscope" itemtype="http://schema.org/ScholarlyArticle"> <div id="header"> <a href="/"><img title="Cold Spring Harbor Perspectives in Biology" alt="Cold Spring Harbor Perspectives in Biology" width="390" height="83" src="/local/img/journal_logo_standalone.gif" /></a> <p id="skip-link"> <a href="#content-block">Skip to main page content</a> </p> <ul class="button-list header-buttons"> <li class="first"><a href="/" title="HOME"><span>HOME</span></a></li> <li><a href="/site/misc/about.xhtml" title="ABOUT"><span>ABOUT</span></a></li> <li><a href="/cgi/collection/" title="SUBJECT COLLECTIONS"><span>SUBJECT COLLECTIONS</span></a></li> <li><a href="/content/by/year" title="ARCHIVE"><span>ARCHIVE</span></a></li> <li><a href="/site/misc/subscribe.xhtml" title="SUBSCRIBE"><span>SUBSCRIBE</span></a></li> <li><a href="/site/misc/adinfo.xhtml" title="ADVERTISE"><span>ADVERTISE</span></a></li> <li><a href="/site/misc/alertland.xhtml" title="ALERTS"><span>ALERTS</span></a></li> <li><a href="/feedback" title="FEEDBACK"><span>FEEDBACK</span></a></li> <li class="last"><a href="/help/" title="HELP"><span>HELP</span></a></li> </ul> <div class="header-qs"> <form class="searchbox" action="/search" method="get"> <div> <label for="header-qs-input" id="header-qs-search-label">Search for Keyword:</label> <input value="" title="Search Cold Spring Harbor Perspectives in Biology" type="text" name="fulltext" id="header-qs-input" /><input type="hidden" name="submit" value="yes" /><label for="header-qs-search" id="header-qs-search-label">GO</label><input value="GO" alt="Link: Go" type="image" id="header-qs-search-go" src="/shared/img/standard-design/design2/go.gif" /> </div> <div class="adv-search-link"><a href="/search">Advanced Search</a></div> </form> </div> <div class="header-ac-elements"> <div class="inst-branding"> </div> <div id="authstring" class="suppress-header-login"> </div> <div id="hdr-login" class="suppress-header-login"></div> </div> <div class="banner-ads"> <ul> <li><a href="/cgi/adclick/?ad=50844&adclick=true&url=http%3A%2F%2Fwww.genelink.com%2Fsirna%2FRNAimain.asp" target="_blank"><img class="adborder0" title="siRNA" src="//cshperspectives.cshlp.org/adsystem/graphics/1691891351805781/cshperspect/Gene%20Link%20siRNA-468x60.gif?ad=50844&adview=true" alt="siRNA" /></a></li> </ul> </div> <div class="bar"> <div class="bar-inner"></div> </div> </div> <div id="content-block"> <div class="article abstract-view " itemprop="articleBody"><span class="highwire-journal-article-marker-start"></span><h1 id="article-title-1" itemprop="headline">Protocells and RNA Self-Replication</h1> <div class="contributors"> <ol class="contributor-list" id="contrib-group-1"> <li class="contributor" id="contrib-1" itemprop="author" itemscope="itemscope" itemtype="http://schema.org/Person"><span class="name" itemprop="name"><a class="name-search" href="/search?author1=Gerald+F.+Joyce&sortspec=date&submit=Submit">Gerald F. Joyce</a></span><a id="xref-aff-1-1" class="xref-aff" href="#aff-1">1</a> and </li> <li class="last" id="contrib-2"><span class="name"><a class="name-search" href="/search?author1=Jack+W.+Szostak&sortspec=date&submit=Submit">Jack W. Szostak</a></span><a id="xref-aff-2-1" class="xref-aff" href="#aff-2">2</a></li> </ol> <ol class="affiliation-list"> <li class="aff"><a id="aff-1" name="aff-1"></a><address><sup>1</sup>The Salk Institute for Biological Studies, La Jolla, California 92037 </address> </li> <li class="aff"><a id="aff-2" name="aff-2"></a><address><sup>2</sup>Howard Hughes Medical Institute and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114 </address> </li> </ol> <ol class="corresp-list"> <li class="corresp" id="corresp-1"><em>Correspondence:</em> <span class="em-link"><span class="em-addr">gjoyce{at}salk.edu</span></span>; <span class="em-link"><span class="em-addr">szostak{at}molbio.mgh.harvard.edu</span></span></li> </ol> </div> <div class="section abstract" id="abstract-1" itemprop="description"> <h2 class="">SUMMARY</h2> <p id="p-3">The general notion of an “RNA world” is that, in the early development of life on the Earth, genetic continuity was assured by the replication of RNA, and RNA molecules were the chief agents of catalytic function. Assuming that all of the components of RNA were available in some prebiotic locale, these components could have assembled into activated nucleotides that condensed to form RNA polymers, setting the stage for the chemical replication of polynucleotides through RNA-templated RNA polymerization. If a sufficient diversity of RNAs could be copied with reasonable rate and fidelity, then Darwinian evolution would begin with RNAs that facilitated their own reproduction enjoying a selective advantage. The concept of a “protocell” refers to a compartment where replication of the primitive genetic material took place and where primitive catalysts gave rise to products that accumulated locally for the benefit of the replicating cellular entity. Replication of both the protocell and its encapsulated genetic material would have enabled natural selection to operate based on the differential fitness of competing cellular entities, ultimately giving rise to modern cellular life. </p> </div> <ul class="copyright-statement"> <li class="fn" id="copyright-statement-1"><a href="http://www.cshperspectives.org/site/misc/terms.xhtml">Copyright © 2018 Cold Spring Harbor Laboratory Press; all rights reserved</a></li> </ul><span class="highwire-journal-article-marker-end"></span></div> <div class="social-bookmarking"> <ul class="social-bookmark-links"> <li class="social-bookmarking-item social-bookmarking-item-citeulike"><a href="/external-ref?tag_url=http://cshperspectives.cshlp.org/cgi/content/long/10/9/a034801&title=Protocells%20and%20RNA%20Self-Replication+--+Joyce%20and%20Szostak%2010%20%289%29+--+cshperspect&doi=10.1101/cshperspect.a034801&link_type=CITEULIKE"><img src="/shared/img/common/social-bookmarking/citeulike.gif" alt="Add to CiteULike" title="CiteULike" /></a><span class="soc-bm-link-text">CiteULike</span></li> <li class="social-bookmarking-item social-bookmarking-item-delicious"><a href="/external-ref?tag_url=http://cshperspectives.cshlp.org/cgi/content/long/10/9/a034801&title=Protocells%20and%20RNA%20Self-Replication+--+Joyce%20and%20Szostak%2010%20%289%29+--+cshperspect&doi=10.1101/cshperspect.a034801&link_type=DEL_ICIO_US"><img src="/shared/img/common/social-bookmarking/delicious.gif" alt="Add to Delicious" title="Delicious" /></a><span class="soc-bm-link-text">Delicious</span></li> <li class="social-bookmarking-item social-bookmarking-item-digg"><a href="/external-ref?tag_url=http://cshperspectives.cshlp.org/cgi/content/long/10/9/a034801&title=Protocells%20and%20RNA%20Self-Replication+--+Joyce%20and%20Szostak%2010%20%289%29+--+cshperspect&doi=10.1101/cshperspect.a034801&link_type=DIGG"><img src="/shared/img/common/social-bookmarking/digg.gif" alt="Add to Digg" title="Digg" /></a><span class="soc-bm-link-text">Digg</span></li> <li class="social-bookmarking-item social-bookmarking-item-facebook"><a href="/external-ref?tag_url=http://cshperspectives.cshlp.org/cgi/content/short/10/9/a034801&title=Protocells%20and%20RNA%20Self-Replication+--+Joyce%20and%20Szostak%2010%20%289%29+--+cshperspect&doi=10.1101/cshperspect.a034801&link_type=FACEBOOK" class="sb-facebook" rel="external-nw"><img src="/shared/img/common/social-bookmarking/facebook.gif" alt="Add to Facebook" title="Facebook" /></a><span class="soc-bm-link-text">Facebook</span></li> <li class="social-bookmarking-item social-bookmarking-item-reddit"><a href="/external-ref?tag_url=http://cshperspectives.cshlp.org/cgi/content/long/10/9/a034801&title=Protocells%20and%20RNA%20Self-Replication+--+Joyce%20and%20Szostak%2010%20%289%29+--+cshperspect&doi=10.1101/cshperspect.a034801&link_type=REDDIT"><img src="/shared/img/common/social-bookmarking/reddit.gif" alt="Add to Reddit" title="Reddit" /></a><span class="soc-bm-link-text">Reddit</span></li> <li class="social-bookmarking-item social-bookmarking-item-twitter"><a href="/external-ref?tag_url=http://cshperspectives.cshlp.org/cgi/content/long/10/9/a034801&title=Protocells%20and%20RNA%20Self-Replication+--+Joyce%20and%20Szostak%2010%20%289%29+--+cshperspect&doi=10.1101/cshperspect.a034801&link_type=TWITTER"><img src="/shared/img/common/social-bookmarking/twitter.gif" alt="Add to Twitter" title="Twitter" /></a><span class="soc-bm-link-text">Twitter</span></li> </ul> <p class="social-bookmarking-help"><a href="/help/social_bookmarks.dtl">What's this?</a></p> </div><span id="related-urls"></span> <br /> <br /> <div class="content-box" id="article-cb-mainy" style="background-color: #f8efd5;"> <div class="cb-contents" style="background-color: #f8efd5;"> <div style="background-color: #f8efd5;"> <h4 class="cb-section-header" style="background-color: #f8efd5;padding-top:12px;"> <span style="background-color: #f8efd5;">Also in this Collection</span> </h4> <ol></ol> <ol class="tweet" id="coll_list_abstract"></ol> </div> </div> </div> <div id="content-end-sb" class="sb-div"></div> </div> <div id="col-2"> <div class="article-nav sidebar-nav"> <a href="/content/10/9/a033613.short" title="Previous article" class="previous">« Previous</a><span class="article-nav-sep"> | </span><a href="/content/10/10/a028431.short" title="Next article" class="next">Next Article »</a> <span class="toc-link"> <a href="/content/10/9.toc" title="Table of Contents">Table of Contents</a> </span> </div> <div class="content-box" id="article-cb-main"> <div class="cb-contents"> <h3 class="cb-contents-header"><span>This Article</span></h3> <div class="cb-section cb-slug"> <ol> <li> <div id="slugline"> doi: <span title="10.1101/cshperspect.a034801" class="slug-doi">10.1101/cshperspect.a034801 </span> <cite> <abbr title="Cold Spring Harbor Perspectives in Biology" class="slug-jnl-abbrev"> Cold Spring Harb. Perspect. Biol.</abbr><span class="slug-pub-date" itemprop="datePublished"> 2018. </span> <span class="slug-vol"> 10: </span> <span class="slug-elocation"> a034801 </span> </cite> <a href="/site/misc/terms.xhtml"><span class="copyright-statement"> <nlm:ext-link xmlns:nlm="http://schema.highwire.org/NLM/Journal" xmlns:l="http://schema.highwire.org/Linking" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:hwp="http://schema.highwire.org/Journal" l:rel="related" l:ref-type="uri" l:ref="http://www.cshperspectives.org/site/misc/terms.xhtml" ext-link-type="uri" xlink:href="http://www.cshperspectives.org/site/misc/terms.xhtml" hwp:id="ext-link-1">Copyright © 2018 Cold Spring Harbor Laboratory Press; all rights reserved</nlm:ext-link></span></a> </div> </li> </ol> </div> <div class="cb-section" id="cb-art-links"> <ol> <li><span class="variant-indicator">» <span>Abstract</span></span><span class="free">Free</span></li> <li class="notice"><a href="/content/10/9/a034801.full" rel="view-full-text">Full Text</a><span class="free">Free</span></li> <li class="notice"><a href="/content/10/9/a034801.full.pdf+html" rel="view-full-text.pdf">Full Text (PDF)</a><span class="free">Free</span></li> </ol> </div> <div class="cb-section collapsible" id="cb-art-cat"> <h4 class="cb-section-header"><span>Article Category</span></h4> <ol> <li> <ul class="subject-headings"> <li>Concept <ul class="subject-sub-headings last-child"> <li><a class="tocsection-search" href="/search?tocsectionid=Cell+Biology&sortspec=date&submit=Submit">Cell Biology</a></li> </ul> </li> </ul> </li> </ol> </div> <div class="cb-section collapsible default-closed" id="cb-art-svcs"> <h4 class="cb-section-header"><span>Services</span></h4> <ol> <li><a href="/cgi/alerts/ctalert?alertType=citedby&addAlert=cited_by&cited_by_criteria_resid=cshperspect%3B10%2F9%2Fa034801&saveAlert=no&return-type=article&return_url=http://cshperspectives.cshlp.org/content/10/9/a034801">Alert me when this article is cited</a></li> <li><a href="/cgi/alerts/ctalert?alertType=correction&addAlert=correction&correction_criteria_value=10/9/a034801&saveAlert=no&return-type=article&return_url=http://cshperspectives.cshlp.org/content/10/9/a034801">Alert me if a correction is posted</a></li> <li><a href="/search?qbe=cshperspect%3B10%2F9%2Fa034801&citation=Joyce%20and%20Szostak%2010%20%289%29&submit=yes">Similar articles in this journal</a></li> <li><a href="/external-ref?access_num=cshperspect%3B10%2F9%2Fa034801&link_type=ISI_RELATEDRECORDS" id="cb-isi-similar-articles">Similar articles in Web of Science</a></li> <li><a href="/external-ref?access_num=30181195&link_type=MED_NBRS">Similar articles in PubMed</a></li> <li><a href="/citmgr?gca=cshperspect%3B10%2F9%2Fa034801">Download to citation manager</a></li> <li><a href="/site/misc/permissions_landing.xhtml"> Permissions </a></li> </ol> </div> <div class="cb-section collapsible default-closed" id="cb-letter-cit"> <h4 class="cb-section-header"><span>Updates/Comments</span></h4> <ol> <li><a href="/letters/submit/cshperspect;10/9/a034801">Submit Updates/Comments</a></li> <li><span class="eletters-no-responses-published">No Updates/Comments published</span></li> </ol> </div> <div class="cb-section collapsible default-closed" id="cb-art-cit"> <h4 class="cb-section-header"><span>Citing Articles</span></h4> <ol> <li><a href="/content/10/9/a034801?cited-by=yes&legid=cshperspect;10/9/a034801#cited-by" id="cb-hw-citing-articles">Load citing article information</a></li> <li><a href="/external-ref?access_num=%2Fcshperspect%2F10%2F9%2Fa034801&link_type=ISI_CITING&accnum_type=native" rel="external-nw" id="cb-isi-citing-articles">Citing articles via Web of Science</a></li> <li><a href="/external-ref?access_num=http://cshperspectives.cshlp.org/content/10/9/a034801.abstract&link_type=GOOGLESCHOLAR">Citing articles via Google Scholar</a></li> </ol> </div> <div class="cb-section collapsible default-closed" id="cb-art-gs"> <h4 class="cb-section-header"><span>Google Scholar</span></h4> <ol> <li><a href="http://scholar.google.com/scholar?q=%22author%3AJoyce%20author%3AG.F.%22">Articles by Joyce, G. F.</a></li> <li><a href="http://scholar.google.com/scholar?q=%22author%3ASzostak%20author%3AJ.W.%22">Articles by Szostak, J. W.</a></li> <li><a href="/external-ref?access_num=http://cshperspectives.cshlp.org/content/10/9/a034801.abstract&link_type=GOOGLESCHOLARRELATED">Search for related content</a></li> </ol> </div> <div class="cb-section collapsible default-closed" id="cb-art-pm"> <h4 class="cb-section-header"><span>PubMed/NCBI</span></h4> <ol> <li><a href="/external-ref?access_num=30181195&link_type=PUBMED">PubMed citation</a></li> <li><a href="/external-ref?access_num=Joyce%20GF&link_type=AUTHORSEARCH">Articles by Joyce, G. F.</a></li> <li><a href="/external-ref?access_num=Szostak%20JW&link_type=AUTHORSEARCH">Articles by Szostak, J. W.</a></li> <li> <div id="cb-entrez-links-placeholder"><a href="/entrez-links/30181195"></a></div> </li> </ol> </div> <div class="cb-section collapsible default-closed" id="cb-art-orcid"> <h4 class="cb-section-header"><span>ORCID</span></h4> <ol> <li class="cb-art-orcid-auth author-link"> <span xmlns="" class="orcid-author">Profile for Joyce, G. F.<a class="orcid-link" href="http://orcid.org/0000-0003-0603-2874">http://orcid.org/0000-0003-0603-2874</a></span> </li> </ol> </div> <div class="cb-section collapsible default-open" id="cb-art-col"> <h4 class="cb-section-header"><span>Subject Collections</span></h4> <ol> <li><a href="/cgi/collection/rna_worlds">RNA Worlds</a></li> </ol> </div> <div class="cb-section collapsible default-closed" id="cb-art-soc"> <h4 class="cb-section-header"><span>Share</span></h4> <ol> <li><a href="/email?gca=cshperspect%3B10%2F9%2Fa034801&current-view-path=/content/10/9/a034801"></a></li> <li> <div class="social-bookmarking"> <ul class="social-bookmark-links"> <li class="social-bookmarking-item social-bookmarking-item-citeulike"><a href="/external-ref?tag_url=http://cshperspectives.cshlp.org/cgi/content/long/10/9/a034801&title=Protocells%20and%20RNA%20Self-Replication+--+Joyce%20and%20Szostak%2010%20%289%29+--+cshperspect&doi=10.1101/cshperspect.a034801&link_type=CITEULIKE"><img src="/shared/img/common/social-bookmarking/citeulike.gif" alt="Add to CiteULike" title="CiteULike" /></a><span class="soc-bm-link-text">CiteULike</span></li> <li class="social-bookmarking-item social-bookmarking-item-delicious"><a href="/external-ref?tag_url=http://cshperspectives.cshlp.org/cgi/content/long/10/9/a034801&title=Protocells%20and%20RNA%20Self-Replication+--+Joyce%20and%20Szostak%2010%20%289%29+--+cshperspect&doi=10.1101/cshperspect.a034801&link_type=DEL_ICIO_US"><img src="/shared/img/common/social-bookmarking/delicious.gif" alt="Add to Delicious" title="Delicious" /></a><span class="soc-bm-link-text">Delicious</span></li> <li class="social-bookmarking-item social-bookmarking-item-digg"><a href="/external-ref?tag_url=http://cshperspectives.cshlp.org/cgi/content/long/10/9/a034801&title=Protocells%20and%20RNA%20Self-Replication+--+Joyce%20and%20Szostak%2010%20%289%29+--+cshperspect&doi=10.1101/cshperspect.a034801&link_type=DIGG"><img src="/shared/img/common/social-bookmarking/digg.gif" alt="Add to Digg" title="Digg" /></a><span class="soc-bm-link-text">Digg</span></li> <li class="social-bookmarking-item social-bookmarking-item-facebook"><a href="/external-ref?tag_url=http://cshperspectives.cshlp.org/cgi/content/short/10/9/a034801&title=Protocells%20and%20RNA%20Self-Replication+--+Joyce%20and%20Szostak%2010%20%289%29+--+cshperspect&doi=10.1101/cshperspect.a034801&link_type=FACEBOOK" class="sb-facebook" rel="external-nw"><img src="/shared/img/common/social-bookmarking/facebook.gif" alt="Add to Facebook" title="Facebook" /></a><span class="soc-bm-link-text">Facebook</span></li> <li class="social-bookmarking-item social-bookmarking-item-reddit"><a href="/external-ref?tag_url=http://cshperspectives.cshlp.org/cgi/content/long/10/9/a034801&title=Protocells%20and%20RNA%20Self-Replication+--+Joyce%20and%20Szostak%2010%20%289%29+--+cshperspect&doi=10.1101/cshperspect.a034801&link_type=REDDIT"><img src="/shared/img/common/social-bookmarking/reddit.gif" alt="Add to Reddit" title="Reddit" /></a><span class="soc-bm-link-text">Reddit</span></li> <li class="social-bookmarking-item social-bookmarking-item-twitter"><a href="/external-ref?tag_url=http://cshperspectives.cshlp.org/cgi/content/long/10/9/a034801&title=Protocells%20and%20RNA%20Self-Replication+--+Joyce%20and%20Szostak%2010%20%289%29+--+cshperspect&doi=10.1101/cshperspect.a034801&link_type=TWITTER"><img src="/shared/img/common/social-bookmarking/twitter.gif" alt="Add to Twitter" title="Twitter" /></a><span class="soc-bm-link-text">Twitter</span></li> </ul> <p class="social-bookmarking-help"><a href="/help/social_bookmarks.dtl">What's this?</a></p> </div> </li> </ol> </div> </div> </div> <style type="text/css"> #col-2 div#cb-biorxiv ol { font-weight:normal; list-style-position:outside; list-style-type:none; list-style-image:url(/publisher/icons/col2-dash.gif); margin-left:4px; } #col-2 #article-cb-mainx { display:visible; } #col-2 div#cb-art-coll ol { font-weight:normal; list-style-position:outside; list-style-type:none; list-style-image:url(/publisher/icons/col2-dash.gif); margin-left:4px; } </style> <div id="col-2-widget-placeholder"> <div class="content-box" id="article-cb-main"> <div class="cb-contents"> <a href="http://biorxiv.org/" rel="external-nw"> <img src="/site/misc/images/biorxiv_ad.gif" /> </a> </div> </div> <div class="content-box" id="author_chat"> <div class="cb-contents"> <h3 class="cb-contents-header"><span>Interview</span></h3> <center><a href="http://mediacentral2.cshl.edu/Symposium75/Symposium75/Interviews/Entries/2010/6/24_TOM_MISTELI.html" onclick="newWindow(this.href, 'popup', 640, 378, 1, 1, 0, 0, 0, 1, 0); return false;"> <img alt="Misteli interview" src="/site/misc/images/misteli3.jpg" style="margin-top:15px;margin-bottom:4px;" /> </a></center> <div style="padding-left:2px;padding-right:2px;"> <a href="http://mediacentral2.cshl.edu/Symposium75/Symposium75/Interviews/Entries/2010/6/24_TOM_MISTELI.html" onclick="newWindow(this.href, 'popup', 640, 380, 1, 1, 0, 0, 0, 1, 0); return false;" style="font-size:8pt;">Click to see an interview with subject collection editor Tom Misteli </a> </div> </div> </div> <div class="content-box" id="author_chat1"> <div class="cb-contents"> <h3 class="cb-contents-header"><span>Interview</span></h3> <center><a href="http://mediacentral.cshl.edu/symp09/cech.mov" onclick="newWindow(this.href, 'popup', 324, 260, 1, 1, 0, 0, 0, 1, 0); return false;"> <img alt="Cech interview" src="/site/misc/images/tcech.jpg" style="margin-top:15px;margin-bottom:4px;" /> </a></center> <div style="padding-left:2px;padding-right:2px;"> <a href="http://mediacentral.cshl.edu/symp09/cech.mov" onclick="newWindow(this.href, 'popup', 324, 260, 1, 1, 0, 0, 0, 1, 0); return false;" style="font-size:8pt;">Click to see an interview with subject collection editor Tom Cech </a> </div> </div> </div> <div class="content-box" id="author_chat2"> <div class="cb-contents"> <h3 class="cb-contents-header"><span>Interview</span></h3> <center><a href="http://mediacentral.cshl.edu/symp09/shapiro.mov" onclick="newWindow(this.href, 'popup', 324, 260, 1, 1, 0, 0, 0, 1, 0); return false;"> <img alt="Shapiro interview" src="/site/misc/images/Shapiro_pic.jpg" style="margin-top:15px;margin-bottom:4px;" /> </a></center> <div style="padding-left:2px;padding-right:2px;"> <a href="http://mediacentral.cshl.edu/symp09/shapiro.mov" onclick="newWindow(this.href, 'popup', 324, 260, 1, 1, 0, 0, 0, 1, 0); return false;" style="font-size:8pt;">Click to see an interview with subject collection editor Lucy Shapiro</a> </div> </div> </div> <div class="content-box" id="author_chat3"> <div class="cb-contents"> <h3 class="cb-contents-header"><span>Interview</span></h3> <center><a href="http://mediacentral2.cshl.edu/Symposium76/Symposium76/Interviews/Entries/2011/6/2_Paolo_Sassone-Corsi.html" onclick="newWindow(this.href, 'popup', 324, 260, 1, 1, 0, 0, 0, 1, 0); return false;"> <img alt="Corsi interview" src="/site/misc/images/corsi.jpg" style="margin-top:15px;margin-bottom:4px;" /> </a></center> <div style="padding-left:2px;padding-right:2px;"> <a href="http://mediacentral2.cshl.edu/Symposium76/Symposium76/Interviews/Entries/2011/6/2_Paolo_Sassone-Corsi.html" onclick="newWindow(this.href, 'popup', 324, 260, 1, 1, 0, 0, 0, 1, 0); return false;" style="font-size:8pt;">Click to see an interview with subject collection editor Paolo Sassone-Corsi</a> </div> </div> </div> <div class="content-box" id="author_chat4"> <div class="cb-contents"> <h3 class="cb-contents-header"><span>Interview</span></h3> <center><a href="http://mediacentral2.cshl.edu/Symposium76/Symposium76/Interviews/Entries/2011/6/3_Richard_Morimoto_1.html" onclick="newWindow(this.href, 'popup', 324, 260, 1, 1, 0, 0, 0, 1, 0); return false;"> <img alt="Corsi interview" src="/site/misc/images/rm.jpg" style="margin-top:15px;margin-bottom:4px;" /> </a></center> <div style="padding-left:2px;padding-right:2px;"> <a href="http://mediacentral2.cshl.edu/Symposium76/Symposium76/Interviews/Entries/2011/6/3_Richard_Morimoto_1.html" onclick="newWindow(this.href, 'popup', 324, 260, 1, 1, 0, 0, 0, 1, 0); return false;" style="font-size:8pt;">Click to see an interview with subject collection editor Richard Morimoto</a> </div> </div> </div> <div class="content-box" id="author_chat5"> <div class="cb-contents"> <h3 class="cb-contents-header"><span>Interview</span></h3> <center><a href="http://mediacentral2.cshl.edu/Symposium77/Estelle.html" onclick="newWindow(this.href, 'popup', 1124, 960, 1, 1, 0, 0, 0, 1, 0); return false;"> <img alt="Estelle interview" src="/site/misc/images/estelle.jpg" style="margin-top:15px;margin-bottom:4px;" /> </a></center> <div style="padding-left:2px;padding-right:2px;"> <a href="http://mediacentral2.cshl.edu/Symposium77/Estelle.html" onclick="newWindow(this.href, 'popup',1124, 960, 1, 1, 0, 0, 0, 1, 0); return false;" style="font-size:8pt;">Click to see an interview with subject collection editor Mark Estelle</a> </div> </div> </div> <div class="content-box" id="author_chat6"> <div class="cb-contents"> <h3 class="cb-contents-header"><span>Interview</span></h3> <center><a href="http://mediacentral2.cshl.edu/Symposium76/Symposium76/Interviews/Entries/2011/6/2_Craig_Thompson_1.html" onclick="newWindow(this.href, 'popup', 1124, 960, 1, 1, 0, 0, 0, 1, 0); return false;"> <img alt="Craig Thompson interview" src="/site/misc/images/ct.jpg" style="margin-top:15px;margin-bottom:4px;" /> </a></center> <div style="padding-left:2px;padding-right:2px;"> <a href="http://mediacentral2.cshl.edu/Symposium76/Symposium76/Interviews/Entries/2011/6/2_Craig_Thompson_1.html" onclick="newWindow(this.href, 'popup',1124, 960, 1, 1, 0, 0, 0, 1, 0); return false;" style="font-size:8pt;">Click to see an interview with Craig Thompson</a> </div> </div> </div> <div class="content-box" id="author_chat7"> <div class="cb-contents"> <h3 class="cb-contents-header"><span>Interview</span></h3> <center><a href="http://mediacentral2.cshl.edu/symposium78/Mathis.html" onclick="newWindow(this.href, 'popup', 1124, 960, 1, 1, 0, 0, 0, 1, 0); return false;"> <img alt="Craig Thompson interview" src="/site/misc/images/dm.jpg" style="margin-top:15px;margin-bottom:4px;" /> </a></center> <div style="padding-left:2px;padding-right:2px;"> <a href="http://mediacentral2.cshl.edu/symposium78/Mathis.html" onclick="newWindow(this.href, 'popup',1124, 960, 1, 1, 0, 0, 0, 1, 0); return false;" style="font-size:8pt;">Click to see an interview with Diane Mathis</a> </div> </div> </div> <style type="text/css"> #col-2 #article-cb-mainx { display:visible; } #col-2 div#cb-art-coll ol { font-weight:normal; list-style-position:outside; list-style-type:none; list-style-image:url(/publisher/icons/col2-dash.gif); margin-left:4px; } </style> <div class="content-box" id="article-cb-mainx"> <div class="cb-contents"> <div class="cb-section collapsible default-open" id="cb-art-coll"> <h4 class="cb-section-header"> <span>In this Collection</span> </h4> <ol class="tweet" id="coll_list"></ol> </div> </div> </div> <script type="text/JavaScript"> var pageid = $("div.hw-gen-page").attr("id"); //alert(pageid); </script> </div> <ul class="tower-ads"> <li class="no-ad tower_col_2"><span> </span></li> </ul> </div> <div id="col-3"> <div class="content-box" id="sidebar-current-issue"> <div class="cb-contents"> <h3 class="cb-contents-header"><span>Current Issue</span></h3> <div class="cb-section"> <ol> <li><span><a href="/content/current" rel="current-issue">February 2025, 17 (2)</a></span></li> </ol> </div> <div class="cb-section"> <ol> <li> <div class="current-issue"><a href="/content/current" rel="current-issue"><img src="/content/17/2.cover.gif" width="67" height="89" alt="Current Issue" /></a></div> </li> </ol> </div> <cb:item xmlns:cb="http://schema.highwire.org/Site/Content-Box"> <div class="cb-section collapsible default-closed" id="sidebar-cur-issue-coverlines"> <h4 class="cb-section-header"> <span>From the cover</span> </h4> <ul> <li> <a>A collage of collection cover images</a> </li> </ul> </div> </cb:item> </div> </div> <div id="sidebar-global-nav"> <ul class="button-list pub-links"> <li class="first"><a href="/content/early/recent" title="Early Release Articles"><span>Early Release Articles</span></a></li> <li><a href="/site/misc/sample.xhtml" title="Featured Articles"><span>Featured Articles</span></a></li> <li><a href="/cgi/collection/" title="Subject Collections"><span>Subject Collections</span></a></li> <li><a href="/content/by/year" title="Archive by Date"><span>Archive by Date</span></a></li> <li><a href="/site/misc/alertland.xhtml" title="Alerts and RSS Feeds"><span>Alerts and RSS Feeds</span></a></li> <li><a href="http://www.cshlpress.com/library/?hwinfo" title="Recommend to Your Library"><span>Recommend to Your Library</span></a></li> <li class="last"><a href="/site/misc/permissions_landing.xhtml" title="Permissions"><span>Permissions</span></a></li> </ul> </div> <ul class="tower-ads"> <li><a href="/cgi/adclick/?ad=50845&adclick=true&url=http%3A%2F%2Fwww.genelink.com%2Fgds%2FGDSintro.asp" target="_blank"><img class="adborder0" title="Fragile X" src="//cshperspectives.cshlp.org/adsystem/graphics/1691891351805781/cshperspect/Fragile_X_2-120x600.gif?ad=50845&adview=true" alt="Fragile X" /></a></li> </ul> <div id="col-3-widget-placeholder" style="padding-top:5px;"> <p style="text-align:center;font-weight:bold;font-size:12px;line-height:15px;margin-top:0;padding-top:0;margin-bottom:0px;color:black;"> <a href="https://www.cshlpress.com/link/molclon4p.htm" style="color:black;">Molecular Cloning<br />The New Edition<br /> <span style=" color: red; ">Buy Now and Save 30%</span> </a> <br />Paperback only<br />(limited time offer) </p> <table style="border-spacing:6px;font-size: 80%;"> <tr> <td style="padding-bottom:6px;padding-top:2px;padding-left:12px;vertical-align:top;"> <a href="https://www.cshlpress.com/link/molclon4p.htm"> <img src="https://www.cshlpress.com/productgraphics/small/Molclon4-1.jpg" alt="Support Research and Save at CSHL Press" /> </a> </td> <td style="padding-bottom:9px;padding-left:6px;padding-right:12px;vertical-align:middle;"> <a href="https://www.cshlpress.com/link/molclon4p.htm">Molecular Cloning: A Laboratory Manual (Fourth Edition)</a> </td> </tr> </table> <div id="ads3" style="padding:0p;margin:0;"> </div> <br /> <br /> <div> <center><img alt="CSH Perspectives" src="/site/misc/covers_hompage/cshlp.jpg" /></center> </div> </div> </div> <div id="footer"> <div class="bar"> <div class="footer-group footer-col-left"> <ul class="button-list footer-buttons"> <li class="first"><a href="/" title="Home"><span>Home</span></a></li> <li><a href="/site/misc/about.xhtml" title="About"><span>About</span></a></li> <li><a href="/cgi/collection/" title="Subject Collections"><span>Subject Collections</span></a></li> <li><a href="/content/by/year" title="Archive"><span>Archive</span></a></li> <li><a href="/site/misc/subscribe.xhtml" title="Subscribe"><span>Subscribe</span></a></li> <li><a href="/site/misc/adinfo.xhtml" title="Advertise"><span>Advertise</span></a></li> <li><a href="/site/misc/alertland.xhtml" title="Alerts"><span>Alerts</span></a></li> <li><a href="/feedback" title="Feedback"><span>Feedback</span></a></li> <li class="last"><a href="/help/" title="Help"><span>Help</span></a></li> </ul> <p class="copyright"><a href="http://www.cshlpress.com/" rel="external-nw">Copyright © 2025 by Cold Spring Harbor Laboratory Press</a></p> </div> <div class="footer-group footer-col-right"> <ul class="issns"> <li> <span>Online ISSN: </span> <span class="issn">1943-0264</span> </li> </ul> </div> </div> <div class="block-2 sb-div"></div> <script type="text/javascript"> document.write(unescape("%3Cscript async src='https://www.googletagmanager.com/gtag/js?id=G-BRRJG2L7NN'%3E%3C/script%3E")); </script><script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-BRRJG2L7NN'); </script> </div><script type="text/javascript"> document.write(unescape("%3Cscript async src='https://www.googletagmanager.com/gtag/js?id=G-1CWXPJ6EVC'%3E%3C/script%3E")); </script><script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-1CWXPJ6EVC'); </script></div> </body> </html>