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Germination medium (GM)
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class="bar-inner"></div> </div> </div> <div id="content-block"> <div xmlns="" class="section-name recipe">Recipe</div> <div class="article fulltext-view" itemprop="articleBody"><span class="highwire-journal-article-marker-start"></span><h1 id="article-title-1" itemprop="headline">Germination medium (GM)</h1> <p id="p-1">1X MS (Murashige and Skoog) salts</p> <p id="p-2">10 g sucrose</p> <p id="p-3"><a href="/lookup/doi/10.1101/pdb.caut554" class="caution"></a>0.5 g 2-[<em>N</em>-morpholino]ethanesulfonic acid (MES) </p> <p id="p-4"><a href="/lookup/doi/10.1101/pdb.caut666" class="caution"></a>KOH (1 N) </p> <p id="p-5">7.5 g agar, tissue-culture-grade</p> <p id="p-6">1 ml Gamborg鈥檚 B5 vitamins (1000X)</p> <p id="p-7">For 1 liter GM, mix MS salts, sucrose, and MES in H<sub>2</sub>O. Adjust pH to 5.7 with 1 N KOH, and add the tissue-culture-grade agar. After autoclaving, cool medium to ~60潞C, and then add Gamborg鈥檚 B5 vitamins. </p><span class="highwire-journal-article-marker-end"></span></div> <div class="social-bookmarking"> <ul class="social-bookmark-links"> <li class="social-bookmarking-item social-bookmarking-item-citeulike"><a href="/external-ref?tag_url=http://cshprotocols.cshlp.org/cgi/content/long/2006/7/pdb.rec10817&title=Germination%20medium%20%28GM%29+--+%202006%20%287%29%3A%20pdb.rec10817+--+0-87969-577-3&doi=10.1101/pdb.rec10817&link_type=CITEULIKE"><img src="/shared/img/common/social-bookmarking/citeulike.gif" alt="Add to CiteULike" title="CiteULike" /></a><span class="soc-bm-link-text">CiteULike</span></li> <li class="social-bookmarking-item social-bookmarking-item-delicious"><a href="/external-ref?tag_url=http://cshprotocols.cshlp.org/cgi/content/long/2006/7/pdb.rec10817&title=Germination%20medium%20%28GM%29+--+%202006%20%287%29%3A%20pdb.rec10817+--+0-87969-577-3&doi=10.1101/pdb.rec10817&link_type=DEL_ICIO_US"><img 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