Contact Angle Measurement, Theory & Relation to Surface Energy

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It will also show you how to get the best results from your measurements." /> <meta property="og:url" content="https://www.ossila.com/pages/contact-angle-theory-measurement" /> <meta property="og:site_name" content="Ossila" /> <meta name="twitter:site" content="@ossilascience"> <meta name="twitter:card" content="summary"> <meta name="twitter:title" content="Contact Angle: A Guide to Theory and Measurement"> <meta name="twitter:description" content="This guide will explain what a contact angle is and how it is measured. It will also show you how the Ossila Contact Angle Goniometer works and how to get the best measurement results. The guide is split into four sections: What is a contact angle? How is contact angle measured? How does the Ossila Contact Angle Goniometer work? What are the tips, tricks and limitations of the measurement? What is a Contact Angle? A contact angle gives us an indication of how well (or how poorly) a liquid will spread over a surface. While formulating an ink, contact angles provide a useful indicator of how a modification to the ink will affect its spreading. A contact angle can be large or small, depending on the physical properties of the materials being investigated. 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You can still place orders by emailing us on <a href="mailto:info@ossila.com">info@ossila.com</a>, but you may experience issues browsing our website. Please consider upgrading to a modern browser for better security and an improved browsing experience.</p> </div> </div>  <main> <div id="informational-page"> <h1>Contact Angle: A Guide to Theory and Measurement</h1> <div class="container"> <img width="1340" src="https://www.ossila.com/cdn/shop/files/Featured-Photo-Dip-close-up.jpg?v=1715351110" loading="lazy" height="280" class="featured-image" alt="Contact Angle: A Guide to Theory and Measurement"> <p>This guide will explain what a contact angle is and how it is measured. It will also show you how the Ossila Contact Angle Goniometer works and how to get the best measurement results. The guide is split into four sections:</p> <ul> <li><a href="#What-is-a-contact-angle">What is a contact angle?</a></li> <li><a href="#How-is-contact-angle-measured">How is contact angle measured?</a></li> <li><a href="#How-does-the-ossila-contact-angle-goniometer-work">How does the Ossila Contact Angle Goniometer work?</a></li> <li><a href="#contact-angle-measurement-tips-and-tricks">What are the tips, tricks and limitations of the measurement?</a></li> </ul> <h2 id="What-is-a-contact-angle">What is a Contact Angle?</h2> <hr> <p>A contact angle gives us an indication of how well (or how poorly) a liquid will spread over a surface. While formulating an ink, contact angles provide a useful indicator of how a modification to the ink will affect its spreading.</p> <p>A contact angle can be large or small, depending on the physical properties of the materials being investigated. The figure below shows three different droplets on a surface. The left-most droplet has a large contact angle, as it does not spread over the solid surface. The right-most droplet has a low contact angle, as it has spread well. This spreading is know as ‘wetting’, and a droplet either ‘wets’ or ‘dewets’ when deposited on a surface.</p> <figure><img width="700" src="https://www.ossila.com/cdn/shop/files/varying-contact-angle.svg?v=1677254121" loading="lazy" height="162" alt="Large and Small Contact Angles"> <figcaption>A varying contact angle</figcaption> </figure> <p>The figure below shows a 2D cross-section of a droplet on a solid surface. Locate the point at which the droplet outline intersects the solid surface. The angle between the droplet outline and the solid surface is the contact angle.</p> <figure><img width="500" src="https://www.ossila.com/cdn/shop/files/contact-angle.svg?v=1677254229" loading="lazy" height="135" alt="Contact Angle"> <figcaption>A contact angle</figcaption> </figure> <p>If we wanted a solution to spread more easily over a substrate, we could alter the solvents used in the formulation, and test them to check if this had increased its wetting capabilities. In this situation, a low contact angle would be the desired outcome.</p> <p>Alternatively, we might be developing a waterproof coating for an item of clothing. In this case, a high contact angle would be desirable. We would alter the coating formulation, and use water droplets to determine which coating was more resistant to wetting.</p> <h3>Surface Tension</h3> <p>The surface tension of a droplet is determined by the interactions between its constituent molecules. The molecules in a droplet of liquid are shown in the figure below. In the bulk of the droplet, intermolecular forces act upon a molecule from all sides equally. However, at the surface of the droplet, there is an absence of liquid molecules on the external side.</p> <figure><img width="500" src="https://www.ossila.com/cdn/shop/files/surface-tension.svg?v=1677254389" loading="lazy" height="285" alt="Droplet Surface Tension"> <figcaption>Surface tension of a droplet</figcaption> </figure> <p>The molecules at the surface are more strongly bound to each other than the molecules in the bulk, as they are not being pulled from all sides. This means that it is more difficult for an object to penetrate the surface than it is for an object to move within the bulk once submerged.</p> <h3>Balanced Forces</h3> <p>There are three boundaries to consider when a droplet is in contact with a solid surface: the solid, the liquid, and the vapour (usually air) surrounding them.</p> <p>The figure below shows a force diagram of the point at which a droplet edge meets a solid surface. The three arrows represent the forces exerted by the surface tensions at three interfaces: liquid-surface, liquid-vapour, and solid-vapour.</p> <figure><img width="500" src="https://www.ossila.com/cdn/shop/files/Contact_Angle_A_Guide_to_Theory_and_Measurement_Balanced_Forces.svg?v=1677851105" loading="lazy" height="258" alt="Contact Angle Forces"> <figcaption>Contact angle forces</figcaption> </figure> <p>Each force is pulling away from the equilibrium point, so if the droplet is in equilibrium, then the forces are balanced and can be described by the following equation:</p> <figure><img width="222" src="https://www.ossila.com/cdn/shop/files/Contact_Angle_Surface_Tensions.png?v=1505211258&width=222&height=23" loading="lazy" height="23" alt="sum of all forces in x direction equation"></figure> <p>Where cos θ gives the x-component of the liquid-vapour surface tension. This can be re-arranged to give:</p> <figure><img width="200" src="https://www.ossila.com/cdn/shop/files/Contact_Angle_Formula.png?v=1505210435&width=200&height=63" loading="lazy" height="63" alt="sum of all forces in x direction equation rearranged to calculate cos theta"></figure> <p>This equation provides some useful informatio:</p> <p>If γ<sub>sv</sub> > γ<sub>ls</sub>, cos θ is positive, and θ is < 90<sup>o</sup> (and the droplet <strong>wets</strong>). This can occur with a <strong>high surface-energy solid</strong> (such as a metal), or a <strong>low surface-tension liquid</strong>.</p> <p>If γ<sub>sv</sub> < γ<sub>ls</sub>, then cos θ will be negative, and θ is therefore > 90<sup>o</sup> (and the droplet <strong>dewets</strong>). This can occur with a <strong>low surface-energy solid</strong>, or a <strong>high surface-tension liquid</strong> (such as water).</p> <p>This may raise the question of how we equate surface energy density (units J/m<sup>2</sup>) with surface tension (units N/m).</p> <figure><img width="476" src="https://www.ossila.com/cdn/shop/files/Surface_Energy.png?v=1505211439&width=476&height=185" loading="lazy" height="185" alt="Surface energy density equation"></figure> <p>Surface energy density and surface tension can therefore be equated.</p> <h2 id="How-is-contact-angle-measured">How is a Contact Angle Measured?</h2> <hr> <p>This section explains the basics of contact angle measurements and how they are usually performed. For specific details on how the <a title="Contact Angle Goniometer" href="/products/contact-angle-goniometer" target="_blank">Ossila Contact Angle Goniometer</a> functions, please see the <a href="#How-does-the-ossila-contact-angle-goniometer-work">third</a> and <a href="#contact-angle-measurement-tips-and-tricks">fourth</a> sections of this guide.</p> <h3>Measurement Overview</h3> <p>The measurement involves two steps: <strong>Recording</strong> and <strong>Analysis.</strong> The equipment required to perform a contact angle measurement can be very simple. The most common method uses three basic components:</p> <ul> <li>A light source</li> <li>A camera</li> <li>A tilting stage</li> </ul> <p>The measurement process is as follows:</p> <ol> <li>The stage is flattened, so that the droplet does not move during deposition.</li> <li>A droplet of liquid is deposited on to the stage.</li> <li>The droplet is illuminated from behind, and an image is recorded by the camera.</li> <li>The image is analysed using code or software, and a contact angle measurement is determined.</li> </ol> <figure><img width="600" src="https://www.ossila.com/cdn/shop/files/Contact_Angle_A_Guide_to_Theory_and_Measurement_Measurement_Overview.svg?v=1677851249" loading="lazy" height="392" alt="Contact Angle Measurement"> <figcaption>Measurement Overview</figcaption> </figure> <h3>Recording an Image for Contact Angle Measurements</h3> <p>The first step in the measurement is to obtain an image of a droplet on a flat surface. There are several factors to consider when designing the optics of the measurement system. Optical components can significantly increase the cost of your setup, even if they are sometimes unnecessary.</p> <p>Let us consider a photograph of a droplet on a substrate. What are the most important aspects of the image for the purposes of our measurement? The figure below shows some of the variables that can affect the quality of the droplet image. They are discussed in more detail below.</p> <figure><img width="500" src="https://www.ossila.com/cdn/shop/files/Contact_Angle_A_Guide_to_Theory_and_Measurement_Recording_an_Image_for_Contact_Angle_Measurements.svg?v=1677851319" loading="lazy" height="511" alt="Variables that determine image quality"> <figcaption>Variables that determine image quality.</figcaption> </figure> <h4>Image Size</h4> <p>Importantly, the image must contain the entire droplet; you can't measure the contact angle if you can't see the edge!</p> <h4>Clarity</h4> <p>You need to be able to see the droplet clearly. The better the clarity of the edges, the more accurately you will be able to measure the contact angle. A 'fuzzy' droplet edge will make it harder to see both the slope of the droplet and the place at which it meets the solid surface.</p> <p>Clarity can be affected by factors such as camera resolution, image magnification, light levels, and contrast with the background.</p> <h4>Camera resolution</h4> <p>Camera resolution determines how many pixels represent the droplet edge. If an image is highly pixelated, it is hard to determine where the edge of the droplet is. For polynomial fitting (discussed later), the fewer the data-points, the less accurate the fit.</p> <h4>Magnification</h4> <p>Image magnification has an impact on resolution. The larger an optical image is, the more pixels it will cover and the higher resolution the image will be.</p> <h4>Contrast</h4> <p>Contrast is important for finding the droplet edge. If there is low contrast, then it will be hard to separate the droplet from its background. If you are using an edge-detection technique, contrast will determine detection accuracy.</p> <h4>Lighting</h4> <p>Light levels can also affect the measurement. You must be able to see the droplet to take a measurement. If the lighting is too light or too dark, the contrast of the droplet and background can be affected.</p> <h4>Depth of Field</h4> <p>You want the entire depth of the droplet to be in focus, otherwise it will be difficult to find the baseline through the blurry front of the droplet. This can be achieved by altering the ‘depth of field’ of the imaging system. The actual focal point of a lens is a specific distance from it. However, there is an area either side of the focal point which you consider to be in focus. This is known as the 'depth of field'. The depth of field can be altered by using an aperture. The image below shows the effect of an aperture on the light entering a camera. As the aperture is reduced in size, the light entering the camera is narrowed.</p> <figure><img width="700" src="https://www.ossila.com/cdn/shop/files/Contact_Angle_A_Guide_to_Theory_and_Measurement_Depth_of_Field.svg?v=1677851085" loading="lazy" height="262" alt="contact angle depth of field"> <figcaption>The effect of an aperture on depth of field.</figcaption> </figure> <p>This results in two changes:</p> <ol> <li>There is less overall light, so you need a bright background light.</li> <li>The depth of field is increased and the ‘in focus’ region becomes longer.</li> </ol> <p>As the aperture is narrowed, the size of the beam changes. If you consider the focal region to be within a certain beam width, you can extend this focal region away from the camera by narrowing the beam. The result of this is shown in the figure above. In the first image, the depth of field starts and ends inside the droplet, so the front and back of the droplet are blurry. In the second image, the depth of field is wider than the whole droplet, so the entire droplet is within focus.</p> <h3>Analysis</h3> <p>Once an image has been obtained, most contact angle measurements follow four basic steps:</p> <div class="row"> <div class="col-sm-6"> <br> <ol> <ol> <li> <strong>Define a baseline</strong> <p>The baseline of the measurement is the horizontal line that represents the solid on which the droplet is being deposited. The contact angle is the angle at the point where this baseline meets the droplet edge.</p> </li> </ol> </ol> <br> <ol> <ol> <li> <strong>Trace the droplet edge</strong> <p>You can’t calculate the contact angle without knowing where the droplet edge meets the baseline. The droplet edge can be drawn by hand, detected programmatically, or estimated based on some assumptions about typical droplet shapes.</p> </li> </ol> </ol> <br> <ol> <ol> <li> <strong>Determine the gradient</strong> <p>Next, you must determine the gradient of the tangent of the droplet edge at the point where it meets the baseline. As with the edge tracing, this can be done by hand with a protractor, or programmatically using software.</p> </li> </ol> </ol> <br> <ol> <li> <strong>Calculate the contact angle</strong> <p>Once you have a baseline and a gradient, you can calculate the angle between them. To do this, you can use the trigonometric functions. For example, if the gradient is 3, then you can use the relationship <strong>tan(θ) = (opposite/adjacent) = (3/1)</strong> to calculate the angle. Once you have the baseline and the droplet edge, you can calculate the angle between them.</p> </li> </ol> <figure><img width="250" src="https://www.ossila.com/cdn/shop/files/Contact_Angle_A_Guide_to_Theory_and_Measurement.svg?v=1677851538" loading="lazy" height="209" alt="angle calculation"> <figcaption>Calculation of contact angle from the gradient of the slope where the droplet edge meets the baseline.</figcaption> </figure> </div> <div class="col-sm-6"> <figure><img width="409.156" src="https://www.ossila.com/cdn/shop/files/Contact_Angle_A_Guide_to_Theory_and_Measurement_Analysis.svg?v=1677851470" loading="lazy" height="815.844" alt="contact angle measurement stages"> <figcaption>Stages of contact angle analysis.</figcaption> </figure> </div> </div> <h2 id="How-does-the-ossila-contact-angle-goniometer-work">How Does the Ossila Contact Angle Goniometer Work?</h2> <hr> <p>In this guide, we will explain how the stages above are performed by the <a title="Contact Angle Goniometer | Ossila" href="/products/contact-angle-goniometer" target="_blank">Ossila Contact Angle Goniometer</a>, along with some tips and tricks to help you get the most out of your contact angle measurements.</p> <h3>1. Defining a baseline</h3> <p>In our Contact Angle Software, the baseline is defined by the user. The figure below shows a screenshot of the software measuring a droplet. The red lines in the image define boundaries of a region of interest (ROI), which is the part of the image that will be used for edge detection. The bottom boundary of this ROI is also the baseline of the measurement. The ROI can be moved by clicking and dragging inside the box, and its size can be changed by dragging the blue handles on the corners and edges.</p> <figure><img width="848.328" src="https://www.ossila.com/cdn/shop/files/contact-angle-software-region-of-interest.png?v=latest&width=848" loading="lazy" alt="setting the contact angle region of interest"> <figcaption>Setting the region of interest, shown as a red box, in the Contact Angle Software. The green line shows the detected edge of the droplet (using Canny edge detection), and the thick red/blue lines show the determined contact angles.</figcaption> </figure> <p>In our software, you can perform edge-detection analysis in real-time, making it easier to determine the correct baseline. By limiting the size of the ROI, you can remove any background objects that may affect detection of the droplet edge.</p> <h3>2. Tracing a droplet edge</h3> <p>One method of determining the droplet's edge is using a <strong>binary threshold</strong>, applied to the area of the image within the region of interest. This threshold changes the image from grey-scale to pure black and white. The software then searches each horizontal line and finds the coordinates of the first black pixel. This edge detection technique is shown in the figure below. The light and dark blue circles indicate where the first black pixels are located in each line as the software searches from left to right (light blue) and then right to left (dark blue).</p> <figure><img width="700" src="https://www.ossila.com/cdn/shop/files/Contact_Angle_A_Guide_to_Theory_and_Measurement-13.svg?v=1677854378" loading="lazy" height="45" alt="contact angle edge detection"> <figcaption>Edge detection of a thresholded droplet image.</figcaption> </figure> <p>The software can also perform <strong>Canny edge detection</strong>, which is a more advanced, multi-step process involving de-noising, calculating intensity gradients, and multiple thresholds.</p> <h3>3. Determining the gradient</h3> <p>The technique described in <a href="#How-is-contact-angle-measured">section 2</a> provides a set of <em>x,y</em> coordinates of the droplet edge, as shown in the figure below <strong>(a)</strong>. Using these coordinates, the Contact Angle software fits a second order polynomial to this edge <strong>(b)</strong>.</p> <p><strong>(c)</strong> shows the gradient of the polynomial at the point where it crosses the baseline. The gradient of the polynomial can be calculated by taking its first derivative. You then input the <em>x</em> value at the point where the polynomial meets the baseline to determine the gradient at that point.</p> <figure><img width="700" src="https://www.ossila.com/cdn/shop/files/Contact_Angle_A_Guide_to_Theory_and_Measurement-12.svg?v=1677854378" loading="lazy" height="494" alt="contact angle goniometer calculation"> <figcaption><strong>(a)</strong> Finding the edge of a droplet. <strong>(b)</strong> Fitting a polynomial to the droplet edge. <strong>(c)</strong> Finding the gradient of the polynomial. <strong>(d)</strong> Calculating contact angle from the gradient.</figcaption> </figure> <h3>4. Calculating the contact angle</h3> <p>Once we have the gradient of the line where it meets the baseline, we can use it to calculate the contact angle. In the figure above, <strong>(d)</strong> shows the components of the equation that was discussed in the previous section. The gradient at the baseline can be thought of as a triangle, with the gradient equal to the length of side a divided by the length of side b. Using the trigonometric relationships, we can determine the angle of the gradient. If the baseline is flat (which it always is), then the angle of the gradient is the same as the contact angle.</p> <h2 id="contact-angle-measurement-tips-and-tricks">What Are the Tips, Tricks, and Limitations of the Measurement?</h2> <hr> <h3>Setting a Baseline</h3> <h4>Find the pointy bits.</h4> <p>The easiest way to set a baseline is to locate the point at which a droplet is mirrored vertically as it reflects off the substrate surface. The figure below shows an image of a droplet on a surface. The part of the droplet below the green line is actually a reflection. If you the baseline at the point where the real image meets its reflection, then you will likely be in the correct place.</p> <figure><img width="848.328" src="https://www.ossila.com/cdn/shop/files/contact-angle-baseline-positioning-droplet.png?v=1725440396&width=848" loading="lazy" alt="contact angle baseline position"> <figcaption>Baseline positioning using droplet reflection.</figcaption> </figure> <h4>Blurry is bad.</h4> <p>As discussed in the very <a href="#What-is-a-contact-angle">first section</a> of this guide, a blurry droplet is difficult to measure. If you can't see the droplet well, then finding the baseline is more difficult. If the droplet is blurry, try screwing and unscrewing the camera lens to focus it, or try moving your substrate closer to and further from the camera - until it is in focus.</p> <h4>Don't go too low.</h4> <p>If the baseline is placed below the correct location, you will begin to detect the edge of the droplet reflection. The figure below shows how this will deform the polynomial fit of the droplet edge, with the edge of the reflected droplet shown by the blue circles. The gradient of the polynomial at the baseline will be negative, and the contact angle will be wrong. Therefore, it is better to set the baseline too high than too low.</p> <figure><img width="500" src="https://www.ossila.com/cdn/shop/files/Contact-Angle-Don_t-go-too-low.svg?v=1677852524" loading="lazy" height="88" alt="The effect of setting the baseline too low"> <figcaption>Setting the baseline too low will effect polynomial fitting.</figcaption> </figure> <h4>Flatten the stage.</h4> <p>The figure below shows the sample stage being tilted towards and away from the camera. Tilting the stage slightly towards the camera can help with baseline positioning, as the reflected droplet is useful for taking a measurement. If the stage is tilted too far towards the camera, the droplet will be viewed from above and the angle measurement will be less accurate. If the stage is tilted away from the camera, then some parts of the droplet will be obscured by the stage, making the measurement inaccurate.</p> <figure><img width="700" src="https://www.ossila.com/cdn/shop/files/Contact_Angle_A_Guide_to_Theory_and_Measurement_Flatten_the_stage.svg?v=1677851624" loading="lazy" height="468" alt="contact angle stage tilt"> <figcaption>Tilting the stage can help align the baseline, however tilting too far can alter the measurement.</figcaption> </figure> <h3>Setting a Region of Interest</h3> <p>Make sure to include the whole droplet within the region of interest. Cropping the droplet edges will result in a wrong part of the droplet being measured. Also, make sure that there are no other objects or droplets within the region of interest, as these will be falsely detected as droplet edges.</p> <h3>Taking a Black and White Threshold</h3> <p>Applying the black/white threshold to an image is most effective when there is a large contrast between the droplet and the background lighting. If a bright light is shone on the droplet from another direction, this can reduce the contrast - which will negatively affect the edge detection. It is therefore best to use the <a title="Ossila Contact Angle Goniometer" href="https://www.ossila.com/products/contact-angle-goniometer">Contact Angle Goniometer</a> in a shaded area, or in a room with low lighting.</p> <h3>Polynomial Fitting</h3> <p>A polynomial fit is only as good as the number of data points it can fit to. In the case of the contact angle measurement, the number of data points is the number of recorded pixels along the droplet edge. As discussed in the <a href="#What-is-a-contact-angle">first section</a>, this is a product of camera resolution, image magnification, and image clarity. When a droplet wets well to a surface, it will spread out very flatly. The flatter a droplet, the fewer pixels you can measure and the fewer data points you have for the polynomial fit. Therefore, when a droplet reaches a certain flatness, you cannot achieve an accurate measurement. 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