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as a Method * define function as object methods * @type {Object} */ window.digitalData = { user: {}, page: {}, merchandising: {}, getQueryParam : function (param) { var index, key, value, hash; var hashes = window.location.href.slice(window.location.href.indexOf('?') + 1).split('&'); for (index = 0; index < hashes.length; index++) { hash = hashes[index].split('='); key = hash[0]; value = hash[1]; if (key.toUpperCase() === param.toUpperCase()) return value; } return ''; }, getValOnce: function (value, cookieName, expiry, timeUnit) { var currentDate = new Date; var currentCookieValue; // timeUnit of 'm' is minutes represented in milliseconds // thus 86400000 = 24 hours = 1 day var interval = timeUnit == 'm' ? 60000 : 86400000; cookieName = cookieName ? cookieName : 'digitaldata_gvo'; value = value ? value : ''; expiry = expiry ? expiry : 0; currentCookieValue = this.getCookie(cookieName); if (value) { currentDate.setTime(currentDate.getTime() + expiry * interval); this.setCookie(cookieName, value, expiry == 0 ? 0 : currentDate); } return value == currentCookieValue ? '' : value; }, getCookie: function (key) { var result = ""; var cookies = document.cookie ? document.cookie.split('; ') : []; for (var i = 0; i < cookies.length; i++) { var parts = cookies[i].split('='); var name = decodeURIComponent(parts.shift()); var cookie = parts ? parts.join('=') : []; // Prevent storing or parsing of a cookie that we couldn't decode. if (cookie && key === name) { // removes surrounding quotes if (cookie.indexOf('"') === 0) { // This is a quoted cookie as according to RFC2068, unescape... cookie = cookie.slice(1, -1).replace(/\\"/g, '"').replace(/\\\\/g, '\\'); } // parse utf8 characters try { cookie = decodeURIComponent(cookie); } catch (e) {} result = cookie; // we found it, don't bother looking at other cookies break; } } return result; }, setCookie : function(name, value, numDays){ if ( !numDays ) numDays = 31; var expires = new Date(); expires.setTime(expires.getTime() + (1000 * 60 * 60 * 24 * numDays)); document.cookie = name+ '=' + escape(value) + '; path=/; expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); }, // generic function to set page attributes setPageAttribute: function (key, value) { this.page[key] = value; }, // display digitalData object displayDigitalData: function () { }, // specific function to add an object to digitalData addDigitDataObject: function (key, object) { this[key] = object; }, addChildObjectAttribute: function(child, key, value){ this[child][key] = value; }, initUserData: function(){ var gigyaCookie = this.getCookie("identity_uid"); this.user.gigyaId = ""; this.user.loginStatus = "anonymous"; if( gigyaCookie != ""){ this.user.gigyaId = gigyaCookie; this.user.loginStatus = "logged in"; } }, /** * Check if init has been initiated once * @type {Boolean} */ merchandisingDataInitted: false, initMerchandisingData: function () { // read cid and icid value from session cookie this.merchandising.icid = this.getCookie('s_ev21_tc'); this.merchandising.cid = this.getCookie('s_cmp_tc'); // EMID value this.merchandising.emid = this.getQueryParam("emid"); // referrer by domain if (typeof document.referrer !== 'undefined' && document.referrer.length > 0) { var masterDomain = this.getMasterDomain(); var refReg = document.referrer; var referrerDomain = refReg.match(/:\/\/([^/]+)\//); if (referrerDomain !== null && referrerDomain[1].indexOf(masterDomain) == -1) { this.merchandising.referrer = referrerDomain; } } document.addEventListener('registerMerchandisingData', function (event) { for (var key in event.detail) { console.log("Listener data copied", key, event.detail[key] ); digitalData.merchandising[key] = event.detail[key]; } // Map some analytics attributes to merchandising session attributes digitalData.merchandising.sessionId = digitalData.merchandising.analyticsVisitorID; digitalData.merchandising.s_vi_low = digitalData.merchandising.analyticsVisitorIDLow; digitalData.merchandising.s_vi_high = digitalData.merchandising.analyticsVisitorIDHigh; console.log("digitalData: Captured registerMerchandisingData event"); digitalData.merchandisingDataInitted = true; // Call emailCampaignRecord if the digitalDataAnalyticsListener object is loaded if (typeof digitalDataAnalyticsListener != "undefined") { console.log("digitalData: Calling email campaign record"); digitalDataAnalyticsListener.emailCampaignRecord(); } }); }, formSubmitEvent: function () { var formId = this.getQueryParam("form-submit-id"); if ((formId) && formId !== "" && formId !== null) { var eventDetail = { detail: { form: { id: formId } } }; var formSubmitEvent = new CustomEvent('formSubmit', eventDetail); console.log('form submit event started'); document.dispatchEvent(formSubmitEvent); console.log('form submit event fired', eventDetail); //flush grepToken from Session Storage sessionStorage.removeItem('grepTokenSO'); } }, modalVideoEvent: function (videoId) { if (((videoId) && videoId !== "" && videoId !== null)) { var eventDetail = { detail: { video: { playerId: videoId } } }; var modalVideoEvent = new CustomEvent('videoPlayerLoaded', eventDetail); document.dispatchEvent(modalVideoEvent); } }, customClickEvent: function (clickDetail) { if (((clickDetail) && clickDetail !== "" && clickDetail !== null)) { var eventDetail = { detail: { CustomLink: { linkName: clickDetail } } } var customClickEvent = new CustomEvent('customLinkClicks', eventDetail); document.dispatchEvent(customClickEvent); } } } })(); window.digitalData.init(); </script>  <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_25"); </script> <script type="text/javascript"> var localizedStrings = { "ACCEPTANCE": "Acceptance of Terms of Use", "BTN_AGREE": "Agree", "BTN_DISAGREE": "Disagree", "PROCESSING_MESSAGE" :"processing ... please wait ...", "ADD_TO_CART_QUANTITY_ERROR":"You must provide a quantity to add an item to the cart", "ADD_TO_CART_VALID_QUANTITY_ERROR":"You must provide a valid quantity to add an item to the cart", "VIEW_CART" :"View cart & checkout", "VIEW_CONTACT_DETAIL" :"View Contact Details", "ADD_TO_CART_NOTIFICATION":"Add to Cart Notification", "UPDATE_CART" :"Update Cart", "PRINCIPAL_INVESTIGATOR":"Principal Investigator", "PRINCIPAL_INVESTIGATOR_TEAM_LEADER":"Principal Investigator or Team Leader", "PRINCIPAL_INVESTIGATOR_CHIEF":"Principal Investigator or Chief", "EMAIL_ERROR_1" :"Please Provide Collegues Email", "EMAIL_ERROR_2" :"Please enter a valid colleague email address", "EMAIL_ERROR_3" :"Please Provide Your Email", "EMAIL_ERROR_4" :"Please enter a valid email address", "ERROR_OCCURED_MESSAGE" :"The following errors occured", "NEXT" :"Next", "PREVIOUS" :"Prev", "IMAGE" :"Image", "SAVE_TO_LIST_ERROR_1" :"Please enter a list name.", "SAVE_TO_LIST_ERROR_2" :"Please enter a different list name that does not include the following: ?,/,\\,%,^,!", "MINICART_ERROR_1" :"Unable to connect to eCommerce system. 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class="details-hero" ng-click="$ctrl.toggleImages('ADVANCED_VERIFICATION')"> <a class="link adv-verification-link animated-scroll" href="/sg/en/home/life-science/antibodies/invitrogen-antibody-validation.html"> <svg class="svg-icon advanced-verification-icon"> <use href="#icon-advanced-verification"></use> </svg> Advanced Verification </a> <p class="adv-verification-detail" onclick="window.THERMO_FISHER.pdp.inp.toggleImagesAdvVerificationSmLtr(event, this)"> This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. </p> </div> <div class="details-hero" onclick="window.THERMO_FISHER.pdp.inp.toggleImagesAdvVerificationPublishedFigures(event, this)"> <a class="link published-link animated-scroll " href="#image-gallery" onclick="window.THERMO_FISHER.pdp.inp.publishedFiguresHero(event, this)"> <svg class="svg-icon published-figures-icon"> <use href="#icon-published-figures"></use> </svg> 9 Published Figures </a> </div> <div class="details-hero"> <a class="link references-link animated-scroll " href="#references-component-id" onclick="window.THERMO_FISHER.pdp.inp.referencesHero(event, this)"> <svg class="svg-icon references-icon"> <use href="#icon-references"></use> </svg> 12 References </a> </div> <div class="details-hero"> <a class="btn hero-btn hero-view-other" href="/antibody/primary/target/tgf beta-1"> View all (62) TGF beta-1 antibodies </a> </div> </div> <div class="links-ab to-show-links-in-sm"> <div class="container"> <div class="link-container"> <a class="link-antibody" href="/order/genome-database/dataSheetPdf?producttype=antibody&productsubtype=antibody_primary&productId=MA5-15065&version=Local" target="_blank" rel="nofollow"> <span> <svg class="svg-icon data-sheet-icon"> <use href="#icon-data-sheet"></use> </svg> </span> Datasheet </a> </div> <div class="link-container"> <a 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Data':[{'sku':'MA5-15065','imageId':'733711','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA515065-TGFbeta1-WB-1-20201020140612.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA515065-TGFbeta1-WB-1-20201020140612.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA515065-TGFbeta1-WB-1-20201020140612.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA515065-TGFbeta1-WB-1-20201020140612.jpg?time\u003d20240507','sortOrder':'4','description':'Western blot was performed using Anti-TGF beta-1 Monoclonal Antibody (F.888.7) (Product # MA5-15065) and 44-53 kDa bands corresponding to latent TGF beta1 was observed to increase in expression upon cisplatin treatment to MDA-MB-231 spheroids and differentiation \u0026 polarization of THP-1 to M2 macrophages. Whole cell extracts (30 µg lysate) of MDA-MB-231 spheroids(Lane 1), cisplatin treated MDA-MB-231 spheroids(Lane 2) and conditioned medium from MDA-MB-231 spheroids(Lane 3), cisplatin treated MDA-MB-231 spheroids(Lane 4) in Fig. a, and whole cell extracts of THP-1(Lane 1), THP-1 differentiated \u0026 polarized to M2 macrophages(Lane 2) in Fig. b, were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1200 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10000 dilution) using the iBright FL 1000 (Product # A32752). Detection was performed using Novex® ECL Reagent Kit (Product # WP20005). Differentiation and polarization to M2 macrophages was obtained by treating THP1 with 320 nm of PMA for 6hrs followed by treatment of PMA (320 nm), IL-4 (20 ng/mL) and IL-13 (20 ng/mL) for 18 hrs.','shortDescription':'Western blot was performed using Anti-TGF beta-1 Monoclonal Antibody (F.888.7) (Product # MA5-15065) and 44-53 kDa bands corresponding to latent TGF beta1 was observed to increase in expression upon cisplatin treatment to MDA-MB-231 spheroids and differentiation \u0026 polarization of THP-1 to M2 macrophages. Whole cell extracts (30 µg lysate) of MDA-MB-231 spheroids(Lane 1), cisplatin treated MDA-MB-231 spheroids(Lane 2) and conditioned medium from MDA-MB-231 spheroids(Lane 3), cisplatin treated MDA-MB-231 spheroids(Lane 4) in Fig. a, and whol... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'TGF beta-1 Antibody in Western Blot (WB)','title':'TGF beta-1 Antibody (MA5-15065) in WB','appAbv':'WB','appName':'Western Blot','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'MA515065-TGFbeta1-WB-1-20201020140612.jpg','benchSciPubmedId':'0'},{'sku':'MA5-15065','imageId':'10052','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA5-15065_WesternBlot_3.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA5-15065_WesternBlot_3.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA5-15065_WesternBlot_3.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA5-15065_WesternBlot_3.jpg?time\u003d20240507','sortOrder':'5','description':'Western blot analysis of TGF-beta in extracts from K-562 and Saos-2 cells using TGF-beta monoclonal antibody (Product # MA5-15065).','shortDescription':'Western blot analysis of TGF-beta in extracts from K-562 and Saos-2 cells using TGF-beta monoclonal antibody (Product # MA5-15065).','altTag':'TGF beta-1 Antibody in Western Blot (WB)','title':'TGF beta-1 Antibody (MA5-15065) in WB','appAbv':'WB','appName':'Western Blot','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'MA5-15065_WesternBlot_3.jpg','benchSciPubmedId':'0'},{'sku':'MA5-15065','imageId':'733710','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA515065-TGFbeta1-siRNA-WB-1-20201020140612.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA515065-TGFbeta1-siRNA-WB-1-20201020140612.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA515065-TGFbeta1-siRNA-WB-1-20201020140612.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA515065-TGFbeta1-siRNA-WB-1-20201020140612.jpg?time\u003d20240507','sortOrder':'6','description':'Knockdown of TGF beta1 (Transforming growth factor beta-1) was achieved by transfecting THP-1 cells with TGF beta1 specific siRNAs (Silencer® select Product # s14056, s14054). For detectable levels of TGF beta1, transfected cells were differentiated and polarized to M2 macrophages. Western blot analysis (Fig. a) was performed using whole cell extracts from the TGF beta1 knockdown M2 macrophages (Lane 6), TGF beta1 control THP-1 (Lane 5), non-targeting scrambled siRNA transfected M2 macrophage (Lane 4), non-targeting scrambled siRNA transfected control THP-1 (Lane 3) and untransfected M2 macrophage (Lane 2) and untransfected control THP-1 (Lane 1) cells. The blot was probed with TGF beta-1 Monoclonal Antibody (F.888.7) (Product # MA5-15065, 1:1000 dilution) and Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to TGF beta1','shortDescription':'Knockdown of TGF beta1 (Transforming growth factor beta-1) was achieved by transfecting THP-1 cells with TGF beta1 specific siRNAs (Silencer® select Product # s14056, s14054). For detectable levels of TGF beta1, transfected cells were differentiated and polarized to M2 macrophages. Western blot analysis (Fig. a) was performed using whole cell extracts from the TGF beta1 knockdown M2 macrophages (Lane 6), TGF beta1 control THP-1 (Lane 5), non-targeting scrambled siRNA transfected M2 macrophage (Lane 4), non-targeting scrambled siRNA transfe... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'TGF beta-1 Antibody in Western Blot (WB)','title':'TGF beta-1 Antibody (MA5-15065) in WB','appAbv':'WB','appName':'Western Blot','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'MA515065-TGFbeta1-siRNA-WB-1-20201020140612.jpg','benchSciPubmedId':'0'},{'sku':'MA5-15065','imageId':'10051','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA5-15065_WesternBlot_2.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA5-15065_WesternBlot_2.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA5-15065_WesternBlot_2.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA5-15065_WesternBlot_2.jpg?time\u003d20240507','sortOrder':'7','description':'Western blot analysis of TGF-beta in extracts from HeLa cells, mock transfected or transfected with TGF-beta1 precursor, using TGF-beta monoclonal antibody (Product # MA5-15065).','shortDescription':'Western blot analysis of TGF-beta in extracts from HeLa cells, mock transfected or transfected with TGF-beta1 precursor, using TGF-beta monoclonal antibody (Product # MA5-15065).','altTag':'TGF beta-1 Antibody in Western Blot (WB)','title':'TGF beta-1 Antibody (MA5-15065) in WB','appAbv':'WB','appName':'Western Blot','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'MA5-15065_WesternBlot_2.jpg','benchSciPubmedId':'0'},{'sku':'MA5-15065','imageId':'10053','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA5-15065_WesternBlot.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA5-15065_WesternBlot.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA5-15065_WesternBlot.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA5-15065_WesternBlot.jpg?time\u003d20240507','sortOrder':'8','description':'Western blot analysis of recombinant active TGF-beta1 using TGF-beta monoclonal antibody (Product # MA5-15065).','shortDescription':'Western blot analysis of recombinant active TGF-beta1 using TGF-beta monoclonal antibody (Product # MA5-15065).','altTag':'TGF beta-1 Antibody in Western Blot (WB)','title':'TGF beta-1 Antibody (MA5-15065) in WB','appAbv':'WB','appName':'Western Blot','type':'Antibody Testing Data','journalText':'','sourceType':'1','benchsci':false,'imageName':'MA5-15065_WesternBlot.jpg','benchSciPubmedId':'0'}],'Published Figures':[{'sku':'MA5-15065','imageId':'879376','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_16655_jcm-10-01889-g005.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_16655_jcm-10-01889-g005.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_16655_jcm-10-01889-g005.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_16655_jcm-10-01889-g005.jpg?time\u003d20240507','sortOrder':'9','description':'Figure 5 SB431542 inhibits TGF-beta1-induced expression of PD-L1 in HCC cells. ( A ) qRT-PCR and ( B ) western blot analysis revealed reduced expression of PD-L1 in PLC/PRF/5 cells after treatment with TGF-beta1 and SB431542. GAPDH was used as loading. ( C ) qRT-PCR and ( D ) western blot analysis revealed reduced expression of PD-L1 following SB431542 treatment in Hep3B cells. GAPDH was used as loading control. ( E ) Fluorescence microscopy revealed reduced expression of PD-L1 following SB431542 treatment in PLC/PRF/5 and Hep3B cells exposed to TGF-beta1 (scale bar \u003d 200 um) ( n \u003d 3, * p \u003c 0.05, ** p \u003c 0.01, *** p \u003c 0.005, **** p \u003c 0.001).','shortDescription':'Figure 5 SB431542 inhibits TGF-beta1-induced expression of PD-L1 in HCC cells. ( A ) qRT-PCR and ( B ) western blot analysis revealed reduced expression of PD-L1 in PLC/PRF/5 cells after treatment with TGF-beta1 and SB431542. GAPDH was used as loading. ( C ) qRT-PCR and ( D ) western blot analysis revealed reduced expression of PD-L1 following SB431542 treatment in Hep3B cells. GAPDH was used as loading con... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'TGF beta-1 Antibody in Immunocytochemistry (ICC/IF)','title':'TGF beta-1 Antibody (MA5-15065) in ICC/IF','appAbv':'ICC/IF','dateTime':'Apr 27, 2021, 4:00:00 AM','appName':'Immunocytochemistry','type':'Published Figures','journalTitle':'Journal of clinical medicine','journalText':'Journal of clinical medicine 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_16655_jcm-10-01889-g005.jpg','benchSciPubmedId':'33925488'},{'sku':'MA5-15065','imageId':'838320','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_12147_fphys-11-580406-g007.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_12147_fphys-11-580406-g007.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_12147_fphys-11-580406-g007.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_12147_fphys-11-580406-g007.jpg?time\u003d20240507','sortOrder':'10','description':'Figure 7 KDM3A inhibits transforming growth factor beta-induced factor 1 (TGIF1) activity and promotes the TGF-beta1/Smad2/3 signaling activation. (A) immunohistochemical staining of TGIF1 in renal tissues of rats in each group; (B) RT-qPCR detection of the mRNA expression of TGIF1 in cells; (C) western blot detection of the protein expression of TGIF1 in cells; (D) chromatin immunoprecipitation (ChIP)-qPCR detection of the level of H3K9me2 modification of the TGIF1 promoter in mesangial cells; (E) immunohistochemical staining of TGF-beta1 expression and Smad2/3 phosphorylation in renal tissues of rats in each group; (F) western blot detection of TGF-beta1 expression and Smad2/3 phosphorylation levels. Variance among more than two groups was analyzed by one-way ( A-C ) or two-way ANOVA ( D-F ), followed by Tukey\u0027s post hoc test. ** p \u003c 0.01, *** p \u003c 0.001.','shortDescription':'Figure 7 KDM3A inhibits transforming growth factor beta-induced factor 1 (TGIF1) activity and promotes the TGF-beta1/Smad2/3 signaling activation. (A) immunohistochemical staining of TGIF1 in renal tissues of rats in each group; (B) RT-qPCR detection of the mRNA expression of TGIF1 in cells; (C) western blot detection of the protein expression of TGIF1 in cells; (D) chromatin immunoprecipitation (ChIP)-qPCR... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'TGF beta-1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)','title':'TGF beta-1 Antibody (MA5-15065) in WB, IHC','appAbv':'IHC','dateTime':'Jan 11, 2021, 5:00:00 AM','appName':'Immunohistochemistry','type':'Published Figures','journalTitle':'Frontiers in physiology','journalText':'Frontiers in physiology 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_12147_fphys-11-580406-g007.jpg','benchSciPubmedId':'33414721'},{'sku':'MA5-15065','imageId':'878421','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_16919_curroncol-28-00200-g005.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_16919_curroncol-28-00200-g005.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_16919_curroncol-28-00200-g005.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_16919_curroncol-28-00200-g005.jpg?time\u003d20240507','sortOrder':'11','description':'Figure 5 Human HCC-derived spheres exhibit mesenchymal phenotype. ( A ) qRT-PCR demonstrated an upregulation of TGF- beta 1 in Hep3B and PLC/PRF/5-derived spheres compared to adherent cells. ( B ) Western blot analysis revealed an upregulation of TGF- beta 1 and p-Smad2/3 in Hep3B and PLC/PRF/5-derived spheres compared to adherent cells and no upregulation of TGF- beta 1 and p-Smad2/3 in HepG2-derived spheres compared to adherent cells. GAPDH was the loading control. Detailed information about Western Blot can be found at Supplementary Materials . ( C ) Transwell migration assay demonstrated higher motility of human HCC-derived spheres compared to adherent cells (scale bar \u003d 500 um). The number of motile cells on the transwell membrane was quantified by measuring the absorbance of Crystal Violet staining. ( n \u003d 3, * p \u003c 0.05, ** p \u003c 0.01, **** p \u003c 0.001, ns: not significant).','shortDescription':'Figure 5 Human HCC-derived spheres exhibit mesenchymal phenotype. ( A ) qRT-PCR demonstrated an upregulation of TGF- beta 1 in Hep3B and PLC/PRF/5-derived spheres compared to adherent cells. ( B ) Western blot analysis revealed an upregulation of TGF- beta 1 and p-Smad2/3 in Hep3B and PLC/PRF/5-derived spheres compared to adherent cells and no upregulation of TGF- beta 1 and p-Smad2/3 in HepG2-derived spher... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'TGF beta-1 Antibody in Western Blot (WB)','title':'TGF beta-1 Antibody (MA5-15065) in WB','appAbv':'WB','dateTime':'Jun 11, 2021, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Current oncology (Toronto, Ont.)','journalText':'Current oncology (Toronto, Ont.) 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_16919_curroncol-28-00200-g005.jpg','benchSciPubmedId':'34208001'},{'sku':'MA5-15065','imageId':'877870','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_16655_jcm-10-01889-g001.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_16655_jcm-10-01889-g001.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_16655_jcm-10-01889-g001.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_16655_jcm-10-01889-g001.jpg?time\u003d20240507','sortOrder':'12','description':'Figure 1 PLC/PRF/5 cells undergo EMT after Sorafenib treatment. ( A ) qRT-PCR revealed decrease in E-cadherin and Occludin and increase in TGF-beta1, N-cadherin, Vimentin, Snai1, and Snai2 after treatment with 6.965 uM Sorafenib for 72 h. ( B ) Western blot analysis showed downregulation of E-cadherin and Occludin and upregulation of Vimentin, N-cadherin, Snai1, Snai2, and TGF-beta1 upon treatment with Sorafenib. GAPDH and Bactin were used as loading controls. ( C ) The migratory capability of PLC/PRF/5 cells was enhanced upon treatment with Sorafenib as revealed by the transwell migration assay (scale bar \u003d 500 um). The number of motile cells was directly proportional to the absorbance of Crystal Violet staining. ( D ) Wound healing assay (scale bar \u003d 500 um) showed higher migratory capacity of Sorafenib treated PLC/PRF/5 cells. Quantitative analysis of the wound area after 24 h Sorafenib treatment relative to the starting wound area at 0 h ( n \u003d 3, * p \u003c 0.05, ** p \u003c 0.01, **** p \u003c 0.001).','shortDescription':'Figure 1 PLC/PRF/5 cells undergo EMT after Sorafenib treatment. ( A ) qRT-PCR revealed decrease in E-cadherin and Occludin and increase in TGF-beta1, N-cadherin, Vimentin, Snai1, and Snai2 after treatment with 6.965 uM Sorafenib for 72 h. ( B ) Western blot analysis showed downregulation of E-cadherin and Occludin and upregulation of Vimentin, N-cadherin, Snai1, Snai2, and TGF-beta1 upon treatment with Sora... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'TGF beta-1 Antibody in Western Blot (WB)','title':'TGF beta-1 Antibody (MA5-15065) in WB','appAbv':'WB','dateTime':'Apr 27, 2021, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Journal of clinical medicine','journalText':'Journal of clinical medicine 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_16655_jcm-10-01889-g001.jpg','benchSciPubmedId':'33925488'},{'sku':'MA5-15065','imageId':'846806','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_13932_ijms-22-03286-g003.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_13932_ijms-22-03286-g003.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_13932_ijms-22-03286-g003.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_13932_ijms-22-03286-g003.jpg?time\u003d20240507','sortOrder':'13','description':'Figure 3 Analysis of fibrosis markers in heart and kidney samples of control and irradiated minipigs. ( A ) Histological analysis of collagen deposition in heart left ventricle (left) and kidney (right) sections using Masson\u0027\u0027s Trichrome staining (blue staining). ( B ) Collagen deposition in the left ventricle was quantified using Sirius Red (red staining) supplemented with Fast Green to visualize cytoplasm; representative photomicrographs (20x magnification; 100 mum scale bar) are shown. ( C ) The percentage of left ventricular area occupied by collagen (field of view) was measured and averages are reported +/- sem (control: n \u003d 3; PBI: n \u003d 7). ( D ) TGF-beta-1 expression was assessed by western blot analysis in heart lysates of irradiated and control animals; Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as loading control. ( E ) Densitometric quantification was performed and values were normalized to GAPDH and presented as averages +/- sem.','shortDescription':'Figure 3 Analysis of fibrosis markers in heart and kidney samples of control and irradiated minipigs. ( A ) Histological analysis of collagen deposition in heart left ventricle (left) and kidney (right) sections using Masson\u0027\u0027s Trichrome staining (blue staining). ( B ) Collagen deposition in the left ventricle was quantified using Sirius Red (red staining) supplemented with Fast Green to visualize cytoplasm... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'TGF beta-1 Antibody in Western Blot (WB)','title':'TGF beta-1 Antibody (MA5-15065) in WB','appAbv':'WB','dateTime':'Mar 23, 2021, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'International journal of molecular sciences','journalText':'International journal of molecular sciences 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_13932_ijms-22-03286-g003.jpg','benchSciPubmedId':'33807089'},{'sku':'MA5-15065','imageId':'838321','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_12147_fphys-11-580406-g008.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_12147_fphys-11-580406-g008.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_12147_fphys-11-580406-g008.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_12147_fphys-11-580406-g008.jpg?time\u003d20240507','sortOrder':'15','description':'Figure 8 Knockdown of TGIF1 promotes the mesangial cell activity. (A) western blot detection of TGIF1 protein expression in cells; (B) western blot detection of TGF-beta1 expression and Smad2/3 phosphorylation levels; (C) detection of cell proliferation by CCK-8 assays; (D) EdU-positive cells in mesangial cells; (E) flow cytometric analysis of mesangial cell death; (F) ELISA detection of the expression of IL-1beta and TNF-alpha in cells; (G) RT-qPCR detection of fibronectin and alpha-SMA in cells; and (H) the immunofluorescence staining of fibronectin and alpha-SMA in cells. Data between two groups were compared using the unpaired t -test ( A , D-H ), whereas variance among more than two groups was analyzed by two-way ANOVA ( B , C ), followed by Tukey\u0027s post hoc test. ** p \u003c 0.01, *** p \u003c 0.001.','shortDescription':'Figure 8 Knockdown of TGIF1 promotes the mesangial cell activity. (A) western blot detection of TGIF1 protein expression in cells; (B) western blot detection of TGF-beta1 expression and Smad2/3 phosphorylation levels; (C) detection of cell proliferation by CCK-8 assays; (D) EdU-positive cells in mesangial cells; (E) flow cytometric analysis of mesangial cell death; (F) ELISA detection of the expression of I... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'TGF beta-1 Antibody in Western Blot (WB)','title':'TGF beta-1 Antibody (MA5-15065) in WB','appAbv':'WB','dateTime':'Jan 11, 2021, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Frontiers in physiology','journalText':'Frontiers in physiology 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_12147_fphys-11-580406-g008.jpg','benchSciPubmedId':'33414721'},{'sku':'MA5-15065','imageId':'838319','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_12147_fphys-11-580406-g004.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_12147_fphys-11-580406-g004.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_12147_fphys-11-580406-g004.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_12147_fphys-11-580406-g004.jpg?time\u003d20240507','sortOrder':'16','description':'Figure 4 MF attenuates inflammation and fibrosis in mesangial cells caused by HG. (A) RT-qPCR detection of the mRNA expression of IL-1beta and TNF-alpha in cells; (B) western blot detection of the protein expression of IL-1beta and TNF-alpha in cells; (C) RT-qPCR detection of the mRNA expression of fibronectin and alpha-SMA in cells; (D) the immunofluorescence staining of fibronectin and alpha-SMA in cells; (E) RT-qPCR detection of the mRNA expression of Collagen IV and TGF-beta1 in cells; and (F) western blot detection of the protein expression of collagen IV and TGF-beta1 in cells. All experiments were repeated three times. Values are expressed as mean +- SD. Variance among more than two groups was compared by two-way ANOVA, followed by Tukey\u0027s post hoc test. ** p \u003c 0.01, *** p \u003c 0.001.','shortDescription':'Figure 4 MF attenuates inflammation and fibrosis in mesangial cells caused by HG. (A) RT-qPCR detection of the mRNA expression of IL-1beta and TNF-alpha in cells; (B) western blot detection of the protein expression of IL-1beta and TNF-alpha in cells; (C) RT-qPCR detection of the mRNA expression of fibronectin and alpha-SMA in cells; (D) the immunofluorescence staining of fibronectin and alpha-SMA in cells;... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'TGF beta-1 Antibody in Western Blot (WB)','title':'TGF beta-1 Antibody (MA5-15065) in WB','appAbv':'WB','dateTime':'Jan 11, 2021, 5:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'Frontiers in physiology','journalText':'Frontiers in physiology 2021 - ','sourceType':'3','benchsci':true,'imageName':'tfs_12147_fphys-11-580406-g004.jpg','benchSciPubmedId':'33414721'},{'sku':'MA5-15065','imageId':'1111405','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_29504_fig4-0300060520905425.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_29504_fig4-0300060520905425.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_29504_fig4-0300060520905425.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_29504_fig4-0300060520905425.jpg?time\u003d20240507','sortOrder':'17','description':'Figure 4. Immunoblotting analysis of MST, TGF-beta1, Smad3, Yap and CTGF among groups over time. Actin was used as an internal reference. a. Protein expression levels of MST, TGF-beta1, Smad3, Yap, CTGF and actin. b. Quantification of MST protein expression in lung tissue. c. Quantification of Smad3 protein expression in lung tissue. d. Quantification of TGF-beta1 protein expression in lung tissue. e. Quantification of Yap protein expression in lung tissue. f. Quantification of CTGF protein expression in lung tissue. Exp vs BC: aa (P \u003c 0.01), a (P \u003c 0.05); Exp vs SY Int: bb (P \u003c 0.01), b (P \u003c 0.05); Exp vs SB Int: **(P \u003c 0.01), *(P \u003c 0.05).Abbreviations: BC, blank control; SY Con, safflower yellow pigment control; SB Con, SB431542 control; Exp, exposure; SY Int, safflower yellow pigment intervention; SB Int, SB431542 intervention; MST, mammalian STE20-like; TGF-beta1, transforming growth factor-beta1; CTGF, connective tissue growth factor.','shortDescription':'Figure 4. Immunoblotting analysis of MST, TGF-beta1, Smad3, Yap and CTGF among groups over time. Actin was used as an internal reference. a. Protein expression levels of MST, TGF-beta1, Smad3, Yap, CTGF and actin. b. Quantification of MST protein expression in lung tissue. c. Quantification of Smad3 protein expression in lung tissue. d. Quantification of TGF-beta1 protein expression in lung tissue. e. Quant... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'TGF beta-1 Antibody in Western Blot (WB)','title':'TGF beta-1 Antibody (MA5-15065) in WB','appAbv':'WB','dateTime':'Sep 1, 2020, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'The Journal of international medical research','journalText':'The Journal of international medical research 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_29504_fig4-0300060520905425.jpg','benchSciPubmedId':'32940100'},{'sku':'MA5-15065','imageId':'1024818','imageUrl':'https://www.thermofisher.com/antibody/images/650/tfs_19621_10.1177_0300060520905425-fig4.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/tfs_19621_10.1177_0300060520905425-fig4.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/tfs_19621_10.1177_0300060520905425-fig4.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/tfs_19621_10.1177_0300060520905425-fig4.jpg?time\u003d20240507','sortOrder':'18','description':'Figure 4. Immunoblotting analysis of MST, TGF-beta1, Smad3, Yap and CTGF among groups over time. Actin was used as an internal reference. a. Protein expression levels of MST, TGF-beta1, Smad3, Yap, CTGF and actin. b. Quantification of MST protein expression in lung tissue. c. Quantification of Smad3 protein expression in lung tissue. d. Quantification of TGF-beta1 protein expression in lung tissue. e. Quantification of Yap protein expression in lung tissue. f. Quantification of CTGF protein expression in lung tissue. Exp vs BC: aa (P \u003c 0.01), a (P \u003c 0.05); Exp vs SY Int: bb (P \u003c 0.01), b (P \u003c 0.05); Exp vs SB Int: **(P \u003c 0.01), *(P \u003c 0.05).Abbreviations: BC, blank control; SY Con, safflower yellow pigment control; SB Con, SB431542 control; Exp, exposure; SY Int, safflower yellow pigment intervention; SB Int, SB431542 intervention; MST, mammalian STE20-like; TGF-beta1, transforming growth factor-beta1; CTGF, connective tissue growth factor.','shortDescription':'Figure 4. Immunoblotting analysis of MST, TGF-beta1, Smad3, Yap and CTGF among groups over time. Actin was used as an internal reference. a. Protein expression levels of MST, TGF-beta1, Smad3, Yap, CTGF and actin. b. Quantification of MST protein expression in lung tissue. c. Quantification of Smad3 protein expression in lung tissue. d. Quantification of TGF-beta1 protein expression in lung tissue. e. Quant... \u003ca href\u003d\'#\' class\u003d\'images-view-more-text\' \u003eView More\u003c/a\u003e','altTag':'TGF beta-1 Antibody in Western Blot (WB)','title':'TGF beta-1 Antibody (MA5-15065) in WB','appAbv':'WB','dateTime':'Sep 1, 2020, 4:00:00 AM','appName':'Western Blot','type':'Published Figures','journalTitle':'The Journal of international medical research','journalText':'The Journal of international medical research 2020 - ','sourceType':'3','benchsci':true,'imageName':'tfs_19621_10.1177_0300060520905425-fig4.jpg','benchSciPubmedId':'32940100'}],'Advanced Verification':[{'sku':'MA5-15065','imageId':'733709','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA515065-TGFbeta1-siRNA-AV-WB-1-20201020140612.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA515065-TGFbeta1-siRNA-AV-WB-1-20201020140612.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA515065-TGFbeta1-siRNA-AV-WB-1-20201020140612.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA515065-TGFbeta1-siRNA-AV-WB-1-20201020140612.jpg?time\u003d20240507','sortOrder':'1','description':'Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. THP-1 cells were transfected with TGF beta1 specific siRNA and then differentiated and polarized to M2 macrophages and decrease in signal intensity was observed in Western Blot application using Anti-TGF beta-1 Monoclonal Antibody (F.888.7) (Product # MA5-15065). {KD}','shortDescription':'Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. THP-1 cells were transfected with TGF beta1 specific siRNA and then differentiated and polarized to M2 macrophages and decrease in signal intensity was observed in Western Blot application using Anti-TGF beta-1 Monoclonal Antibody (F.888.7) (Product # MA5-15065). {KD}','altTag':'TGF beta-1 Antibody','title':'TGF beta-1 Antibody (MA5-15065)','appAbv':'KD','appName':'Knockdown','type':'Advanced Verification','journalText':'','sourceType':'2','benchsci':false,'imageName':'MA515065-TGFbeta1-siRNA-AV-WB-1-20201020140612.jpg','benchSciPubmedId':'0'},{'sku':'MA5-15065','imageId':'733708','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA515065-TGFbeta1-AV1-WB-1-20201020140612.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA515065-TGFbeta1-AV1-WB-1-20201020140612.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA515065-TGFbeta1-AV1-WB-1-20201020140612.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA515065-TGFbeta1-AV1-WB-1-20201020140612.jpg?time\u003d20240507','sortOrder':'2','description':'Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of TGF beta1 was observed in THP-1 cells differentiated and polarized to M2 macrophage compared to THP-1 control using Anti-TGF beta-1 Monoclonal Antibody (F.888.7) (Product # MA5-15065) in Western Blot. {RE}','shortDescription':'Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of TGF beta1 was observed in THP-1 cells differentiated and polarized to M2 macrophage compared to THP-1 control using Anti-TGF beta-1 Monoclonal Antibody (F.888.7) (Product # MA5-15065) in Western Blot. {RE}','altTag':'TGF beta-1 Antibody','title':'TGF beta-1 Antibody (MA5-15065)','appAbv':'RE','appName':'Relative expression','type':'Advanced Verification','journalText':'','sourceType':'2','benchsci':false,'imageName':'MA515065-TGFbeta1-AV1-WB-1-20201020140612.jpg','benchSciPubmedId':'0'},{'sku':'MA5-15065','imageId':'733707','imageUrl':'https://www.thermofisher.com/antibody/images/650/MA515065-TGFbeta1-AV2-WB-1-20201020140612.jpg?time\u003d20240507','imageUrlFullSize':'https://www.thermofisher.com/antibody/images/650/MA515065-TGFbeta1-AV2-WB-1-20201020140612.jpg?time\u003d20240507','imageUrlMidSize':'https://www.thermofisher.com/antibody/images/250/MA515065-TGFbeta1-AV2-WB-1-20201020140612.jpg?time\u003d20240507','imageUrlSmallSize':'https://www.thermofisher.com/antibody/images/150/MA515065-TGFbeta1-AV2-WB-1-20201020140612.jpg?time\u003d20240507','sortOrder':'3','description':'Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using TGF beta-1 Monoclonal Antibody (F.888.7) (Product # MA5-15065), shows upregulation of TGF beta 1 upon cisplatin treatment to MDA-MB-231 spheroids where it is reported to reduce DNA damage in tumors [doi:10.1006/excr.1998.4261]. {TM}','shortDescription':'Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using TGF beta-1 Monoclonal Antibody (F.888.7) (Product # MA5-15065), shows upregulation of TGF beta 1 upon cisplatin treatment to MDA-MB-231 spheroids where it is reported to reduce DNA damage in tumors [doi:10.1006/excr.1998.4261]. {TM}','altTag':'TGF beta-1 Antibody','title':'TGF beta-1 Antibody (MA5-15065)','appAbv':'TM','appName':'Cell treatment','type':'Advanced Verification','journalText':'','sourceType':'2','benchsci':false,'imageName':'MA515065-TGFbeta1-AV2-WB-1-20201020140612.jpg','benchSciPubmedId':'0'}]}" image-displayed-id="'733711'" product-sub-type="'antibody_primary'" prod-type="'antibody'" page-type="PDP" product-id="'MA5-15065'"> <div class="tabs"> <ul class="nav nav-pills"> <li class="tab" onclick="window.THERMO_FISHER.inp.onTabClick(event, this)" data-tabname="Antibody Testing Data"> <a class="tab-text"> Antibody Testing Data (5) </a> </li> <li class="tab" onclick="window.THERMO_FISHER.inp.onTabClick(event, this)" data-tabname="Published Figures"> <a 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class="gallery-count"> <p> FIGURE: <span class="current-img"> 1 </span> / <span class="img-total-count"> 17</span></p> </div> <div class = "verification-certificate hide" > <span class="icon-certification"></span> <span class="adv-cert-text">{{ $ctrl.currentElement.advancedVerification.fullName }}</span> <div class="cert-provider">{{ $ctrl.currentElement.advancedVerification.text }}</div> </div> <h2 class="product-name" ng-show="!$ctrl.isVideo"> TGF beta-1 Antibody (MA5-15065) in WB </h2> <div ng-show="!$ctrl.isVideo"> <div class="image-desc"> <div class="product-desc-text viewing-more-long-description"> <span class="image-journal-text" > </span> <span class="short-desc"> Western blot was performed using Anti-TGF beta-1 Monoclonal Antibody (F.888.7) (Product # MA5-15065) and 44-53 kDa bands corresponding to latent TGF beta1 was observed to increase in expression upon cisplatin treatment to MDA-MB-231 spheroids and differentiation & polarization of THP-1 to M2 macrophages. Whole cell extracts (30 µg lysate) of MDA-MB-231 spheroids(Lane 1), cisplatin treated MDA-MB-231 spheroids(Lane 2) and conditioned medium from MDA-MB-231 spheroids(Lane 3), cisplatin treated MDA-MB-231 spheroids(Lane 4) in Fig. a, and whol... <a href="#" class="images-view-more-text" >View More</a> </span> <span class="verification-info hide" ng-if="$ctrl.hasVerificationMethod"> <a rel="nofollow" ng-href="{{$ctrl.currentElement.advancedVerification.uri}}" >{{ $ctrl.currentElement.advancedVerification.fullName }} validation info.</a> </span> <a href="#" class="images-view-more-text hide" ng-if="$ctrl.showViewMore" onclick="window.THERMO_FISHER.inp.openImageGalleryModal(event, this)"> View more </a> </div> </div> <div class="img-desc-footer"> <div class="benchsci-pubmed-footer"> <div ng-cloak ng-if="$ctrl.currentElement.benchsci || $ctrl.sourceType === '3'" class="images-benchsci-footer"> <div class="images-benchsci-link" tabindex="0"> <span>Published figure supplied by</span> <span class="icon-benchsci-logo"> <svg id="Layer_1" data-name="Layer 1" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 89.75 23.21"><defs><style>.cls-1{fill:#222222;}</style></defs><title>benchsci-logo</title><g id="logo-blue-copy-3"><g id="logo-blue-copy-2"><path id="path14" class="cls-1" d="M34.54,11.49a2.45,2.45,0,0,0,1.59-2.34c0-1.69-1.21-2.94-3.32-2.94H28.88v10.9h4.21a3.06,3.06,0,0,0,3.37-3A2.57,2.57,0,0,0,34.54,11.49ZM31,8h1.49c1,0,1.57.53,1.57,1.36a1.36,1.36,0,0,1-1.55,1.37H31Zm1.72,7.35H31V12.47h1.75a1.42,1.42,0,0,1,1.62,1.46C34.34,14.79,33.72,15.34,32.69,15.34Z"/><path id="path16" class="cls-1" d="M41.34,9.32a3.8,3.8,0,0,0-3.72,4,3.83,3.83,0,0,0,3.91,4.05A3.39,3.39,0,0,0,45,15l-1.71-.51a1.67,1.67,0,0,1-1.72,1.15,1.85,1.85,0,0,1-1.91-1.73H45s0-.34,0-.63C45.09,10.81,43.69,9.32,41.34,9.32Zm-1.66,3.15A1.63,1.63,0,0,1,41.37,11a1.52,1.52,0,0,1,1.69,1.49Z"/><path id="path18" class="cls-1" d="M50.58,9.35a2.48,2.48,0,0,0-2.18,1.14v-1h-2v7.57h2.05V12.75a1.42,1.42,0,0,1,1.41-1.57,1.34,1.34,0,0,1,1.4,1.52v4.42h2V12.35C53.32,10.68,52.46,9.35,50.58,9.35Z"/><path id="path20" class="cls-1" d="M58.2,15.47a1.94,1.94,0,0,1-1.94-2.14,1.92,1.92,0,0,1,1.91-2.12,1.64,1.64,0,0,1,1.67,1.27l1.83-.61a3.45,3.45,0,0,0-3.55-2.55,3.83,3.83,0,0,0-2.82,1.16,3.88,3.88,0,0,0-1.1,2.85,3.9,3.9,0,0,0,4,4,3.5,3.5,0,0,0,3.53-2.55l-1.8-.6A1.72,1.72,0,0,1,58.2,15.47Z"/><path id="path22" class="cls-1" d="M67,9.35a2.72,2.72,0,0,0-2.07.83V6h-2V17.12h2V12.67a1.43,1.43,0,0,1,1.42-1.48,1.34,1.34,0,0,1,1.4,1.53v4.41h2V12.35A2.67,2.67,0,0,0,67,9.35Z"/><path id="path24" class="cls-1" d="M75.82,11.05l-1.63-.35a1.58,1.58,0,0,1-1.4-1.59A1.93,1.93,0,0,1,74.9,7.3a2.21,2.21,0,0,1,2.3,1.86l1.36-.48a3.73,3.73,0,0,0-7.29.54,3,3,0,0,0,2.67,3l1.55.33c1.09.24,1.63.91,1.63,1.7,0,.95-.74,1.74-2.25,1.74a2.51,2.51,0,0,1-1.79-.63,2.45,2.45,0,0,1-.82-1.71l-1.45.46a3.79,3.79,0,0,0,4.08,3.24c2.32,0,3.76-1.52,3.76-3.22C78.65,12.59,77.63,11.45,75.82,11.05Z"/><path id="path26" class="cls-1" d="M83.25,16A2.33,2.33,0,0,1,81,13.41a2.31,2.31,0,0,1,2.24-2.59,1.93,1.93,0,0,1,2,1.57l1.31-.55a3.22,3.22,0,0,0-3.29-2.35,3.7,3.7,0,0,0-3.72,3.92,3.75,3.75,0,0,0,3.74,3.94A3.41,3.41,0,0,0,86.59,15l-1.28-.55A2,2,0,0,1,83.25,16Z"/><path id="path28" class="cls-1" d="M88.69,5.89a1,1,0,1,0,.75.29A1,1,0,0,0,88.69,5.89Z"/><polygon id="rect30" class="cls-1" points="87.99 9.72 89.42 9.72 89.42 17.11 87.99 17.11 87.99 9.72"/><path id="path32" class="cls-1" d="M14.58,8.87A4.1,4.1,0,0,0,15.8,6H14.31a2.62,2.62,0,0,1-.76,1.87L11.66,9.7l-2-2A2.61,2.61,0,0,1,9,6H7.53A4.09,4.09,0,0,0,8.74,8.87l1.87,1.87L8.74,12.61a4.08,4.08,0,0,0-1.21,2.92v2.29H9.18V16.73h1.66v1.09h1.64V16.73h1.65v1.09H15.8V15.53a4.11,4.11,0,0,0-1.21-2.92l-1.38-1.36-1,1.05,1.37,1.36a2.63,2.63,0,0,1,.77,1.58H9a2.68,2.68,0,0,1,.76-1.58Z"/><path id="path34" class="cls-1" d="M11.61,23.21a11.61,11.61,0,1,1,11.6-11.6A11.6,11.6,0,0,1,11.61,23.21Zm0-21.89A10.29,10.29,0,1,0,21.9,11.61,10.28,10.28,0,0,0,11.61,1.32Z"/></g></g></svg> </span> <span id="bechsci-tooltip-{{$ctrl.productId}}" class="bechsci-tooltip-icon" data-toggle="tooltip" ng-click="$ctrl.showBechSciTooltip()" close-tooltip="$ctrl.closeBechSciTooltip()" data-html="true" title="Clicking this link will redirect you to the <a href='https://www.benchsci.com/' target='_blank'> BenchSci</a> website that provides third-party scientific content. 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itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA515065-TGFbeta1-WB-1-20201020140612.jpg?time=20240507?time=20240507" alt="TGF beta-1 Antibody in Western Blot (WB)" title="TGF beta-1 Antibody (MA5-15065) in WB"> <figcaption itemprop="caption">Western blot was performed using Anti-TGF beta-1 Monoclonal Antibody (F.888.7) (Product # MA5-15065) and 44-53 kDa bands corresponding to latent TGF beta1 was observed to increase in expression upon cisplatin treatment to MDA-MB-231 spheroids and differentiation & polarization of THP-1 to M2 macrophages. Whole cell extracts (30 µg lysate) of MDA-MB-231 spheroids(Lane 1), cisplatin treated MDA-MB-231 spheroids(Lane 2) and conditioned medium from MDA-MB-231 spheroids(Lane 3), cisplatin treated MDA-MB-231 spheroids(Lane 4) in Fig. a, and whole cell extracts of THP-1(Lane 1), THP-1 differentiated & polarized to M2 macrophages(Lane 2) in Fig. b, were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1200 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10000 dilution) using the iBright FL 1000 (Product # A32752). Detection was performed using Novex® ECL Reagent Kit (Product # WP20005). Differentiation and polarization to M2 macrophages was obtained by treating THP1 with 320 nm of PMA for 6hrs followed by treatment of PMA (320 nm), IL-4 (20 ng/mL) and IL-13 (20 ng/mL) for 18 hrs.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA5-15065_WesternBlot_3.jpg?time=20240507?time=20240507" alt="TGF beta-1 Antibody in Western Blot (WB)" title="TGF beta-1 Antibody (MA5-15065) in WB"> <figcaption itemprop="caption">Western blot analysis of TGF-beta in extracts from K-562 and Saos-2 cells using TGF-beta monoclonal antibody (Product # MA5-15065).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA515065-TGFbeta1-siRNA-WB-1-20201020140612.jpg?time=20240507?time=20240507" alt="TGF beta-1 Antibody in Western Blot (WB)" title="TGF beta-1 Antibody (MA5-15065) in WB"> <figcaption itemprop="caption">Knockdown of TGF beta1 (Transforming growth factor beta-1) was achieved by transfecting THP-1 cells with TGF beta1 specific siRNAs (Silencer® select Product # s14056, s14054). For detectable levels of TGF beta1, transfected cells were differentiated and polarized to M2 macrophages. Western blot analysis (Fig. a) was performed using whole cell extracts from the TGF beta1 knockdown M2 macrophages (Lane 6), TGF beta1 control THP-1 (Lane 5), non-targeting scrambled siRNA transfected M2 macrophage (Lane 4), non-targeting scrambled siRNA transfected control THP-1 (Lane 3) and untransfected M2 macrophage (Lane 2) and untransfected control THP-1 (Lane 1) cells. The blot was probed with TGF beta-1 Monoclonal Antibody (F.888.7) (Product # MA5-15065, 1:1000 dilution) and Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to TGF beta1</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA5-15065_WesternBlot_2.jpg?time=20240507?time=20240507" alt="TGF beta-1 Antibody in Western Blot (WB)" title="TGF beta-1 Antibody (MA5-15065) in WB"> <figcaption itemprop="caption">Western blot analysis of TGF-beta in extracts from HeLa cells, mock transfected or transfected with TGF-beta1 precursor, using TGF-beta monoclonal antibody (Product # MA5-15065).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA5-15065_WesternBlot.jpg?time=20240507?time=20240507" alt="TGF beta-1 Antibody in Western Blot (WB)" title="TGF beta-1 Antibody (MA5-15065) in WB"> <figcaption itemprop="caption">Western blot analysis of recombinant active TGF-beta1 using TGF-beta monoclonal antibody (Product # MA5-15065).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_16655_jcm-10-01889-g005.jpg?time=20240507?time=20240507" alt="TGF beta-1 Antibody in Immunocytochemistry (ICC/IF)" title="TGF beta-1 Antibody (MA5-15065) in ICC/IF"> <figcaption itemprop="caption">Figure 5 SB431542 inhibits TGF-beta1-induced expression of PD-L1 in HCC cells. ( A ) qRT-PCR and ( B ) western blot analysis revealed reduced expression of PD-L1 in PLC/PRF/5 cells after treatment with TGF-beta1 and SB431542. GAPDH was used as loading. ( C ) qRT-PCR and ( D ) western blot analysis revealed reduced expression of PD-L1 following SB431542 treatment in Hep3B cells. GAPDH was used as loading control. ( E ) Fluorescence microscopy revealed reduced expression of PD-L1 following SB431542 treatment in PLC/PRF/5 and Hep3B cells exposed to TGF-beta1 (scale bar = 200 um) ( n = 3, * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_12147_fphys-11-580406-g007.jpg?time=20240507?time=20240507" alt="TGF beta-1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)" title="TGF beta-1 Antibody (MA5-15065) in WB, IHC"> <figcaption itemprop="caption">Figure 7 KDM3A inhibits transforming growth factor beta-induced factor 1 (TGIF1) activity and promotes the TGF-beta1/Smad2/3 signaling activation. (A) immunohistochemical staining of TGIF1 in renal tissues of rats in each group; (B) RT-qPCR detection of the mRNA expression of TGIF1 in cells; (C) western blot detection of the protein expression of TGIF1 in cells; (D) chromatin immunoprecipitation (ChIP)-qPCR detection of the level of H3K9me2 modification of the TGIF1 promoter in mesangial cells; (E) immunohistochemical staining of TGF-beta1 expression and Smad2/3 phosphorylation in renal tissues of rats in each group; (F) western blot detection of TGF-beta1 expression and Smad2/3 phosphorylation levels. Variance among more than two groups was analyzed by one-way ( A-C ) or two-way ANOVA ( D-F ), followed by Tukey's post hoc test. ** p < 0.01, *** p < 0.001.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_16919_curroncol-28-00200-g005.jpg?time=20240507?time=20240507" alt="TGF beta-1 Antibody in Western Blot (WB)" title="TGF beta-1 Antibody (MA5-15065) in WB"> <figcaption itemprop="caption">Figure 5 Human HCC-derived spheres exhibit mesenchymal phenotype. ( A ) qRT-PCR demonstrated an upregulation of TGF- beta 1 in Hep3B and PLC/PRF/5-derived spheres compared to adherent cells. ( B ) Western blot analysis revealed an upregulation of TGF- beta 1 and p-Smad2/3 in Hep3B and PLC/PRF/5-derived spheres compared to adherent cells and no upregulation of TGF- beta 1 and p-Smad2/3 in HepG2-derived spheres compared to adherent cells. GAPDH was the loading control. Detailed information about Western Blot can be found at Supplementary Materials . ( C ) Transwell migration assay demonstrated higher motility of human HCC-derived spheres compared to adherent cells (scale bar = 500 um). The number of motile cells on the transwell membrane was quantified by measuring the absorbance of Crystal Violet staining. ( n = 3, * p < 0.05, ** p < 0.01, **** p < 0.001, ns: not significant).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_16655_jcm-10-01889-g001.jpg?time=20240507?time=20240507" alt="TGF beta-1 Antibody in Western Blot (WB)" title="TGF beta-1 Antibody (MA5-15065) in WB"> <figcaption itemprop="caption">Figure 1 PLC/PRF/5 cells undergo EMT after Sorafenib treatment. ( A ) qRT-PCR revealed decrease in E-cadherin and Occludin and increase in TGF-beta1, N-cadherin, Vimentin, Snai1, and Snai2 after treatment with 6.965 uM Sorafenib for 72 h. ( B ) Western blot analysis showed downregulation of E-cadherin and Occludin and upregulation of Vimentin, N-cadherin, Snai1, Snai2, and TGF-beta1 upon treatment with Sorafenib. GAPDH and Bactin were used as loading controls. ( C ) The migratory capability of PLC/PRF/5 cells was enhanced upon treatment with Sorafenib as revealed by the transwell migration assay (scale bar = 500 um). The number of motile cells was directly proportional to the absorbance of Crystal Violet staining. ( D ) Wound healing assay (scale bar = 500 um) showed higher migratory capacity of Sorafenib treated PLC/PRF/5 cells. Quantitative analysis of the wound area after 24 h Sorafenib treatment relative to the starting wound area at 0 h ( n = 3, * p < 0.05, ** p < 0.01, **** p < 0.001).</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_13932_ijms-22-03286-g003.jpg?time=20240507?time=20240507" alt="TGF beta-1 Antibody in Western Blot (WB)" title="TGF beta-1 Antibody (MA5-15065) in WB"> <figcaption itemprop="caption">Figure 3 Analysis of fibrosis markers in heart and kidney samples of control and irradiated minipigs. ( A ) Histological analysis of collagen deposition in heart left ventricle (left) and kidney (right) sections using Masson''s Trichrome staining (blue staining). ( B ) Collagen deposition in the left ventricle was quantified using Sirius Red (red staining) supplemented with Fast Green to visualize cytoplasm; representative photomicrographs (20x magnification; 100 mum scale bar) are shown. ( C ) The percentage of left ventricular area occupied by collagen (field of view) was measured and averages are reported +/- sem (control: n = 3; PBI: n = 7). ( D ) TGF-beta-1 expression was assessed by western blot analysis in heart lysates of irradiated and control animals; Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as loading control. ( E ) Densitometric quantification was performed and values were normalized to GAPDH and presented as averages +/- sem.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_12147_fphys-11-580406-g008.jpg?time=20240507?time=20240507" alt="TGF beta-1 Antibody in Western Blot (WB)" title="TGF beta-1 Antibody (MA5-15065) in WB"> <figcaption itemprop="caption">Figure 8 Knockdown of TGIF1 promotes the mesangial cell activity. (A) western blot detection of TGIF1 protein expression in cells; (B) western blot detection of TGF-beta1 expression and Smad2/3 phosphorylation levels; (C) detection of cell proliferation by CCK-8 assays; (D) EdU-positive cells in mesangial cells; (E) flow cytometric analysis of mesangial cell death; (F) ELISA detection of the expression of IL-1beta and TNF-alpha in cells; (G) RT-qPCR detection of fibronectin and alpha-SMA in cells; and (H) the immunofluorescence staining of fibronectin and alpha-SMA in cells. Data between two groups were compared using the unpaired t -test ( A , D-H ), whereas variance among more than two groups was analyzed by two-way ANOVA ( B , C ), followed by Tukey's post hoc test. ** p < 0.01, *** p < 0.001.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_12147_fphys-11-580406-g004.jpg?time=20240507?time=20240507" alt="TGF beta-1 Antibody in Western Blot (WB)" title="TGF beta-1 Antibody (MA5-15065) in WB"> <figcaption itemprop="caption">Figure 4 MF attenuates inflammation and fibrosis in mesangial cells caused by HG. (A) RT-qPCR detection of the mRNA expression of IL-1beta and TNF-alpha in cells; (B) western blot detection of the protein expression of IL-1beta and TNF-alpha in cells; (C) RT-qPCR detection of the mRNA expression of fibronectin and alpha-SMA in cells; (D) the immunofluorescence staining of fibronectin and alpha-SMA in cells; (E) RT-qPCR detection of the mRNA expression of Collagen IV and TGF-beta1 in cells; and (F) western blot detection of the protein expression of collagen IV and TGF-beta1 in cells. All experiments were repeated three times. Values are expressed as mean +- SD. Variance among more than two groups was compared by two-way ANOVA, followed by Tukey's post hoc test. ** p < 0.01, *** p < 0.001.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_29504_fig4-0300060520905425.jpg?time=20240507?time=20240507" alt="TGF beta-1 Antibody in Western Blot (WB)" title="TGF beta-1 Antibody (MA5-15065) in WB"> <figcaption itemprop="caption">Figure 4. Immunoblotting analysis of MST, TGF-beta1, Smad3, Yap and CTGF among groups over time. Actin was used as an internal reference. a. Protein expression levels of MST, TGF-beta1, Smad3, Yap, CTGF and actin. b. Quantification of MST protein expression in lung tissue. c. Quantification of Smad3 protein expression in lung tissue. d. Quantification of TGF-beta1 protein expression in lung tissue. e. Quantification of Yap protein expression in lung tissue. f. Quantification of CTGF protein expression in lung tissue. Exp vs BC: aa (P < 0.01), a (P < 0.05); Exp vs SY Int: bb (P < 0.01), b (P < 0.05); Exp vs SB Int: **(P < 0.01), *(P < 0.05).Abbreviations: BC, blank control; SY Con, safflower yellow pigment control; SB Con, SB431542 control; Exp, exposure; SY Int, safflower yellow pigment intervention; SB Int, SB431542 intervention; MST, mammalian STE20-like; TGF-beta1, transforming growth factor-beta1; CTGF, connective tissue growth factor.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/tfs_19621_10.1177_0300060520905425-fig4.jpg?time=20240507?time=20240507" alt="TGF beta-1 Antibody in Western Blot (WB)" title="TGF beta-1 Antibody (MA5-15065) in WB"> <figcaption itemprop="caption">Figure 4. Immunoblotting analysis of MST, TGF-beta1, Smad3, Yap and CTGF among groups over time. Actin was used as an internal reference. a. Protein expression levels of MST, TGF-beta1, Smad3, Yap, CTGF and actin. b. Quantification of MST protein expression in lung tissue. c. Quantification of Smad3 protein expression in lung tissue. d. Quantification of TGF-beta1 protein expression in lung tissue. e. Quantification of Yap protein expression in lung tissue. f. Quantification of CTGF protein expression in lung tissue. Exp vs BC: aa (P < 0.01), a (P < 0.05); Exp vs SY Int: bb (P < 0.01), b (P < 0.05); Exp vs SB Int: **(P < 0.01), *(P < 0.05).Abbreviations: BC, blank control; SY Con, safflower yellow pigment control; SB Con, SB431542 control; Exp, exposure; SY Int, safflower yellow pigment intervention; SB Int, SB431542 intervention; MST, mammalian STE20-like; TGF-beta1, transforming growth factor-beta1; CTGF, connective tissue growth factor.</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA515065-TGFbeta1-siRNA-AV-WB-1-20201020140612.jpg?time=20240507?time=20240507" alt="TGF beta-1 Antibody" title="TGF beta-1 Antibody (MA5-15065)"> <figcaption itemprop="caption">Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. THP-1 cells were transfected with TGF beta1 specific siRNA and then differentiated and polarized to M2 macrophages and decrease in signal intensity was observed in Western Blot application using Anti-TGF beta-1 Monoclonal Antibody (F.888.7) (Product # MA5-15065). {KD}</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA515065-TGFbeta1-AV1-WB-1-20201020140612.jpg?time=20240507?time=20240507" alt="TGF beta-1 Antibody" title="TGF beta-1 Antibody (MA5-15065)"> <figcaption itemprop="caption">Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of TGF beta1 was observed in THP-1 cells differentiated and polarized to M2 macrophage compared to THP-1 control using Anti-TGF beta-1 Monoclonal Antibody (F.888.7) (Product # MA5-15065) in Western Blot. {RE}</figcaption> </figure> </div> <div> <hr> <figure itemprop="image" itemscope itemtype="http://schema.org/ImageObject"> <img src="https://www.thermofisher.com/antibody/images/650/MA515065-TGFbeta1-AV2-WB-1-20201020140612.jpg?time=20240507?time=20240507" alt="TGF beta-1 Antibody" title="TGF beta-1 Antibody (MA5-15065)"> <figcaption itemprop="caption">Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using TGF beta-1 Monoclonal Antibody (F.888.7) (Product # MA5-15065), shows upregulation of TGF beta 1 upon cisplatin treatment to MDA-MB-231 spheroids where it is reported to reduce DNA damage in tumors [doi:10.1006/excr.1998.4261]. {TM}</figcaption> </figure> </div> </div> </noscript> <div id="tf-related-products-container-new"></div> <div class="product-details"> <div class="container"> <div class="row"> <div class="large-span-12 medium-span-12 small-span-12"> <h2 class="pdp-sub-heading product_details_heading">Product Details</h2></div> <div class="hidden-sku"> <h2>MA5-15065</h2> </div> <div class="product-detail-section tested-app-section new-tbl-design product-detail-section table-spec"> <div class="product-container-head"> <div class="product-item"> Applications </div> <div class="product-item dilution-lbl-head "> Tested Dilution </div> <div class="product-item published-applications"> Publications </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Western Blot (WB)</h2></div> <div class="product-item "> <span class="dilution-lbl">1:1,000</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#WB-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 9 publications</span> <span class="mobile-view">9 publications</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Immunohistochemistry (IHC)</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#IHC-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 1 publication</span> <span class="mobile-view">1 publication</span></a> </div> </div> <div class="product-container-body"> <div class="product-item"> <h2>Immunocytochemistry (ICC/IF)</h2></div> <div class="product-item "> <span class="dilution-lbl dilution-lbl-dash">-</span> </div> <div class="product-item"> <a class="animated-scroll" onclick="window.THERMO_FISHER.pdp.inp.handleClickReferenceProductDetails(event,this,'a[href="#ICCIF-content&quot]')" data-target="References" href="#references-component-id"> <span class="desktop-view">View 2 publications</span> <span class="mobile-view">2 publications</span></a> </div> </div> </div> <div class="product-detail-section tested-app-section new-tbl-design product-specific-section table-spec"> <div class="product-container-head"> <div class="product-item">Product Specifications</div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Species Reactivity</h2> </div> <div class="product-item left-flex-cls"> <span title="Homo sapiens">Human</span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Published species</h2> </div> <div class="product-item left-flex-cls"> <span>Human,</span> <span>Mouse,</span> <span>Rat</span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2> Host/Isotype</h2> </div> <div class="product-item left-flex-cls"> <span title="Oryctolagus cuniculus">Rabbit / IgG</span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Class</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Monoclonal </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Type</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Antibody </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Clone</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> F.888.7 </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Immunogen </h2> </div> <div class="product-item left-flex-cls">Synthetic peptide corresponding to a region in the carboxy terminus of TGF-ß1 protein <script type="text/javascript"> if (typeof window.$mangular === 'undefined' || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = '[{"targetFamily":"TGF beta-1","uniProtId":"P01137-1","ncbiNodeId":"9606","antigenRange":"390","antigenLength":"390","antigenImageFileName":"MA5-15065_TGF_beta-1_P01137-1_rabbit.svg","antigenImageFileNamePDP":"MA5-15065_TGF_beta-1_P01137-1_rabbit_PDP.jpeg","sortOrder":"1"}]'; $mangular.isB2BCMGT = '"false"'; $mangular.isEpitopesModalImageMultiSizeEnabled = 'true'; </script> <div class="antigen-link" ng-click="$ctrl.openModal()"> <a href="">View immunogen <span class="antigen-link-icon"> <?xml version="1.0" encoding="utf-8"?>  <svg version="1.1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px" viewBox="0 0 20 20" style="enable-background:new 0 0 20 20;" xml:space="preserve"> <style type="text/css"> .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;} </style> <g id="Layer_1"> <line class="st0" x1="1.2" y1="2.5" x2="17.6" y2="2.5"/> <path d="M16.4,2.5"/> <g> <path d="M15.4,13.5"/> <path d="M15,14.9l2.8-2.8L15,9.3c0,0,0,0.9,0,1.9h-13V13h13C15.1,14,15.1,14.9,15,14.9z"/> <path d="M4.9,19.3l-2.8-2.8l2.8-2.8c0,0,0,0.9,0,1.9h13v1.8h-13C4.9,18.4,4.9,19.3,4.9,19.3z"/> </g> <g> <path d="M15.4,3.4"/> <path d="M15,6l2.8-2.8L15,0.3c0,0,0,0.9,0,1.9h-13V4h13C15.1,5.1,15.1,5.9,15,6z"/> <path d="M4.9,10.4L2.1,7.6l2.8-2.8c0,0,0,0.9,0,1.9h13v1.8h-13C4.9,9.5,4.9,10.4,4.9,10.4z"/> </g> <g> <path d="M2.8,8.1C1.3,8.1,0,6.9,0,5.4s1.3-2.7,2.8-2.7v1C1.8,3.6,1,4.4,1,5.4s0.8,1.8,1.8,1.8V8.1z"/> </g> <g> <path d="M17.2,12.6c1.5,0,2.8-1.2,2.8-2.8s-1.3-2.8-2.8-2.8v1c1,0,1.8,0.8,1.8,1.8s-0.8,1.8-1.8,1.8V12.6z"/> </g> <g> <path d="M2.8,17.1c-1.5,0-2.8-1.2-2.8-2.8s1.3-2.8,2.8-2.8v1c-1,0-1.8,0.8-1.8,1.8s0.8,1.8,1.8,1.8V17.1z"/> </g> </g> <g id="Layer_1_copy"> <path class="st1" d="M17.4,12.5c1.4-0.1,2.6-1.3,2.6-2.7c0-1.3-0.9-2.4-2.1-2.7V6.7h-13c0-1.1,0-1.9,0-1.9L2.6,7.1 C1.7,7,1,6.3,1,5.4C1,4.6,1.5,4,2.1,3.7V4h13c0,1,0,1.9,0,1.9l2.8-2.8L15,0.3c0,0,0,0.9,0,1.9h-13v0.4C0.9,3,0,4.1,0,5.4 C0,6.8,1.1,8,2.6,8.1l2.3,2.3c0,0,0-0.9,0-1.9h13V8.2C18.5,8.4,19,9.1,19,9.8c0,0.9-0.7,1.7-1.7,1.7L15,9.3c0,0,0,0.9,0,1.9h-13 v0.4C0.9,12,0,13,0,14.3c0,1.5,1.2,2.6,2.6,2.7l2.3,2.3c0,0,0-0.9,0-1.9h13v-1.8h-13c0-1.1,0-1.9,0-1.9L2.6,16 C1.7,15.9,1,15.2,1,14.3c0-0.7,0.5-1.3,1.1-1.6V13h13c0,1,0,1.9,0,1.9L17.4,12.5z"/> </g> </svg> </span> </a> </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Conjugate</h2> </div> <div class="product-item left-flex-cls"> <a ng-if="$ctrl.checkConjugate('Unconjugated ') && ''.trim() != ''" href="" ng-attr-target="{{$ctrl.getRequestCustomLinkTarget()}}" class="conjugate-value">Unconjugated </a> <span ng-if="($ctrl.checkConjugate('Unconjugated ') && ''.trim() == '')" class="conjugate-value">Unconjugated </span> <span ng-if="!$ctrl.checkConjugate('Unconjugated ')" class="conjugate-value">Unconjugated </span> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Form</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Liquid </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Concentration</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> 194 µg/mL </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Purification</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Affinity chromatography </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Storage buffer</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Contains</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> <0.02% sodium azide </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Storage conditions</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> -20°C </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"> <h2>Shipping conditions</h2> </div> <div class="product-item left-flex-cls"> <div class="product-value"> Wet ice </div> </div> </div> <div class="product-container-body pcb1-2"> <div class="product-item"><h2>RRID <span id="rrid-tooltip" class="rrid-tooltip-icon" ng-include="'/order/genome-database/webapp/assets/core/img/svg/mono_tooltip.svg'" data-toggle="tooltip" ng-click="$ctrl.showRridTooltip()" close-tooltip="$ctrl.closeRridTooltip()" title="Research Resource Identifiers (#RRID) are ID numbers assigned to help researchers cite key resources in the biomedical literature to improve transparency of research methods."> </span></h2> </div> <div class="product-item left-flex-cls">AB_10984297</div> </div> </div> <div class="product-specific-details"> <div class="product-specific-details-tbl product-specific-add-border" > <div class="product-info-para"> <h4 class="product-info-para-heading"> Product Specific Information </h4> <p>It is not recommended to aliquot this antibody.</p> </div> <div class="product-info-para"> <h4 class="product-info-para-heading"> Target Information </h4> <p class="product-specific-target-info">TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.</p> </div> <p class="product-details-regulatoryStmt">For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.</p> </div> <div class="product-detail-panel-builder"> <tf-promo-ad ng-if="$ctrl.showPromoAd" show-promo-ad="$ctrl.showPromoAd"></tf-promo-ad> </div> </div> </div> </div> </div> <div id="rootPdpDocLoader"></div> <div id="tf-related-products-container" ng-show="emptyRelatedProductsWidget === false" > <related-products-container ng-if="showRelatedProductsWidget === true" base-url="'/api/magellan/related-products-ui/widget/v2/'" product-type="'antibody'" product-sub-type="'primary'" product-id="'MA515065'" country-code="'sg'" iso-language="'en'" > </related-products-container> </div> <div class="tf-pdp-divider" ng-if="emptyRelatedProductsWidget === false" ></div> <div class="references reference-section-3" id="references-component-id"> <div id="references-section-id"> <reference-section ng-if="loadReferences" default-display-card-limit="3" reference-count="12" sub-type="'Antibody'" application-data='{"citationApplications":[{"applicationAbbreviation":"WB","citations":[{"title":"Late Health Effects of Partial Body Irradiation Injury in a Minipig Model Are Associated with Changes in Systemic and Cardiac IGF-1 Signaling.","summary":"Published figure using TGF beta-1 monoclonal antibody (Product # MA5-15065) in Western Blot","authors":"Hritzo B,Aghdam SY,Legesse B,Kaur A,Cao M,Boerma M,Chakraborty N,Dimitrov G,Gautam A,Hammamieh R,Wilkins W,Tsioplaya A,Holmes-Hampton GP,Moroni M","literatureId":"33807089","journal":"International journal of molecular sciences","volume":"22","publishedDate":"Mar 23, 2021, 4:00:00 AM","url":"http://dx.doi.org/10.3390/ijms22063286","benchSci":true,"imageURL":"https://www.thermofisher.com/antibody/images/250/tfs_13932_ijms-22-03286-g003.jpg?time\u003d20240507","imageId":"846806","journalAbbr":"Int J Mol Sci","sortOrder":5,"species":[],"speciesDilutionMap":{"Not Applicable":"Not Cited"},"abbreviationToNameMap":{"WB":"Western 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Blot"},"newSpeciesDilutionMap":{"Mouse~WB~1:500":"1:500"}}],"citationsCount":9,"speciesList":["Human","Mouse","Rat"],"journals":["Curr Oncol","J Clin Med","Front Physiol","Int J Mol Sci","J Int Med Res","Front Pharmacol","Acta Biomater","Mol Carcinog","Biomedicines"],"speciesMap":{"Human":4,"Mouse":1,"Rat":2},"journalsMap":{"Curr Oncol":1,"J Clin Med":1,"Front Physiol":1,"Int J Mol Sci":1,"J Int Med Res":1,"Front Pharmacol":1,"Acta Biomater":1,"Mol Carcinog":1,"Biomedicines":1},"sortedMap":{}},{"applicationAbbreviation":"IHC","citations":[],"citationsCount":1,"speciesList":[],"journals":["Front Physiol"],"speciesMap":{},"journalsMap":{"Front Physiol":1},"sortedMap":{}},{"applicationAbbreviation":"ICC/IF","citations":[],"citationsCount":2,"speciesList":["Human","Mouse"],"journals":["Biophys J","J Clin Med"],"speciesMap":{"Human":1,"Mouse":1},"journalsMap":{"Biophys J":1,"J Clin Med":1},"sortedMap":{}}]}'></reference-section> </div> <noscript> <div itemscope itemtype="https://schema.org/ScholarlyArticle"> <h2>Product References</h2> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/35740412"> <div itemprop="name">Modulatory Effects of Estradiol and Its Mixtures with Ligands of GPER and PPAR on MAPK and PI3K/Akt Signaling Pathways and Tumorigenic Factors in Mouse Testis Explants and Mouse Tumor Leydig Cells.</div> </a> <div itemprop="about">Biomedicines</div> <div itemprop="author">Gorowska-Wojtowicz E,Duliban M,Kotula-Balak M,Bilinska B</div> <div itemprop="description">MA5-15065 was used in Western Blotting to suggest that a balanced estrogen level and its action together with proper GPER and PPAR signaling play a key role in the maintenance of testis homeostasis.</div> <div itemprodp="datePublished">Sun Jun 12 00:00:00 EDT 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34882856"> <div itemprop="name">LINC02381 aggravates breast cancer through the miR-1271-5p/FN1 axis to activate PI3K/AKT pathway.</div> </a> <div itemprop="about">Molecular carcinogenesis</div> <div itemprop="author">Huang S,Huang P,Wu H,Wang S,Liu G</div> <div itemprop="description">MA5-15065 was used in Western Blotting to conclude that LINC02381 exerts carcinogenic effects in BC by the miR-1271-5p/FN1 axis to activate PI3K/AKT pathway.</div> <div itemprodp="datePublished">Tue Mar 01 00:00:00 EST 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/36386153"> <div itemprop="name">The novel anti-colitic effect of β-adrenergic receptors <i>via</i> modulation of PS1/BACE-1/Aβ axis and NOTCH signaling in an ulcerative colitis model.</div> </a> <div itemprop="about">Frontiers in pharmacology</div> <div itemprop="author">Nasser S,Abdallah DM,Ahmed KA,Abdel-Mottaleb Y,El-Abhar HS</div> <div itemprop="description">MA5-15065 was used in Western Blotting to investigate the impact of mirabegron (β3-agonist) and/or carvedilol (β1/β2 antagonist) on iodoacetamide-induced ulcerative colitis with emphasis on the novel pathomechanism of the PS1/BACE-1/Aβ axis in ulcerative colitis, and its relation to the inflammatory cascade, fibrotic processes, and the gut barrier dysfunction.</div> <div itemprodp="datePublished">Fri Nov 18 00:00:00 EST 2022</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34208001"> <div itemprop="name">Dual Targeting of Sorafenib-Resistant HCC-Derived Cancer Stem Cells.</div> </a> <div itemprop="about">Current oncology (Toronto, Ont.)</div> <div itemprop="author">Shrestha R,Bridle KR,Cao L,Crawford DHG,Jayachandran A</div> <div itemprop="description">MA5-15065 was used in Western Blot to conclude that the combined targeting of EMT and immune checkpoint molecules with Sorafenib can effectively target the CSC tumour subpopulation.</div> <div itemprodp="datePublished">Fri Jun 11 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/34208001"> <div itemprop="name">Dual Targeting of Sorafenib-Resistant HCC-Derived Cancer Stem Cells.</div> </a> <div itemprop="about">Current oncology (Toronto, Ont.)</div> <div itemprop="author">Shrestha R,Bridle KR,Cao L,Crawford DHG,Jayachandran A</div> <div itemprop="description">MA5-15065 was used in Western Blot to conclude that the combined targeting of EMT and immune checkpoint molecules with Sorafenib can effectively target the CSC tumour subpopulation.</div> <div itemprodp="datePublished">Fri Jun 11 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33807089"> <div itemprop="name">Late Health Effects of Partial Body Irradiation Injury in a Minipig Model Are Associated with Changes in Systemic and Cardiac IGF-1 Signaling.</div> </a> <div itemprop="about">International journal of molecular sciences</div> <div itemprop="author">Hritzo B,Aghdam SY,Legesse B,Kaur A,Cao M,Boerma M,Chakraborty N,Dimitrov G,Gautam A,Hammamieh R,Wilkins W,Tsioplaya A,Holmes-Hampton GP,Moroni M</div> <div itemprop="description">Published figure using TGF beta-1 monoclonal antibody (Product # MA5-15065) in Western Blot</div> <div itemprodp="datePublished">Tue Mar 23 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33925488"> <div itemprop="name">Combined Inhibition of TGF-β1-Induced EMT and PD-L1 Silencing Re-Sensitizes Hepatocellular Carcinoma to Sorafenib Treatment.</div> </a> <div itemprop="about">Journal of clinical medicine</div> <div itemprop="author">Shrestha R,Prithviraj P,Bridle KR,Crawford DHG,Jayachandran A</div> <div itemprop="description">MA5-15065 was used in Western Blotting to investigate whether combined inhibition of EMT and immune checkpoints can re-sensitise hepatocellular carcinoma to Sorafenib treatment.</div> <div itemprodp="datePublished">Tue Apr 27 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33925488"> <div itemprop="name">Combined Inhibition of TGF-β1-Induced EMT and PD-L1 Silencing Re-Sensitizes Hepatocellular Carcinoma to Sorafenib Treatment.</div> </a> <div itemprop="about">Journal of clinical medicine</div> <div itemprop="author">Shrestha R,Prithviraj P,Bridle KR,Crawford DHG,Jayachandran A</div> <div itemprop="description">MA5-15065 was used in Western Blotting to investigate whether combined inhibition of EMT and immune checkpoints can re-sensitise hepatocellular carcinoma to Sorafenib treatment.</div> <div itemprodp="datePublished">Tue Apr 27 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33925488"> <div itemprop="name">Combined Inhibition of TGF-β1-Induced EMT and PD-L1 Silencing Re-Sensitizes Hepatocellular Carcinoma to Sorafenib Treatment.</div> </a> <div itemprop="about">Journal of clinical medicine</div> <div itemprop="author">Shrestha R,Prithviraj P,Bridle KR,Crawford DHG,Jayachandran A</div> <div itemprop="description">MA5-15065 was used in Western Blotting to investigate whether combined inhibition of EMT and immune checkpoints can re-sensitise hepatocellular carcinoma to Sorafenib treatment.</div> <div itemprodp="datePublished">Tue Apr 27 00:00:00 EDT 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32940100"> <div itemprop="name">Role of the Hippo signaling pathway in safflower yellow pigment treatment of paraquat-induced pulmonary fibrosis.</div> </a> <div itemprop="about">The Journal of international medical research</div> <div itemprop="author">Li H,Kan B,Song L,Liu Y,Jian X</div> <div itemprop="description">MA5-15065 was used in Western Blotting to elucidate the molecular mechanisms by which safflower yellow (SY) mediates therapeutic effects in rats with paraquat intoxication-induced pulmonary fibrosis.</div> <div itemprodp="datePublished">Tue Sep 01 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/32940100"> <div itemprop="name">Role of the Hippo signaling pathway in safflower yellow pigment treatment of paraquat-induced pulmonary fibrosis.</div> </a> <div itemprop="about">The Journal of international medical research</div> <div itemprop="author">Li H,Kan B,Song L,Liu Y,Jian X</div> <div itemprop="description">MA5-15065 was used in Western Blotting to elucidate the molecular mechanisms by which safflower yellow (SY) mediates therapeutic effects in rats with paraquat intoxication-induced pulmonary fibrosis.</div> <div itemprodp="datePublished">Tue Sep 01 00:00:00 EDT 2020</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33414721"> <div itemprop="name">Magnoflorine Ameliorates Inflammation and Fibrosis in Rats With Diabetic Nephropathy by Mediating the Stability of Lysine-Specific Demethylase 3A.</div> </a> <div itemprop="about">Frontiers in physiology</div> <div itemprop="author">Chang L,Wang Q,Ju J,Li Y,Cai Q,Hao L,Zhou Y</div> <div itemprop="description">Published figure using TGF beta-1 monoclonal antibody (Product # MA5-15065) in Western Blot</div> <div itemprodp="datePublished">Mon Jan 11 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/33414721"> <div itemprop="name">Magnoflorine Ameliorates Inflammation and Fibrosis in Rats With Diabetic Nephropathy by Mediating the Stability of Lysine-Specific Demethylase 3A.</div> </a> <div itemprop="about">Frontiers in physiology</div> <div itemprop="author">Chang L,Wang Q,Ju J,Li Y,Cai Q,Hao L,Zhou Y</div> <div itemprop="description">Published figure using TGF beta-1 monoclonal antibody (Product # MA5-15065) in Western Blot</div> <div itemprodp="datePublished">Mon Jan 11 00:00:00 EST 2021</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/26200861"> <div itemprop="name">Palladin mediates stiffness-induced fibroblast activation in the tumor microenvironment.</div> </a> <div itemprop="about">Biophysical journal</div> <div itemprop="author">McLane JS,Ligon LA</div> <div itemprop="description">MA5-15065 was used in Immunocytochemistry to identify the actin-associated protein palladin as a key node in these signaling pathways that result in fibroblast activation.</div> <div itemprodp="datePublished">Tue Jul 21 00:00:00 EDT 2015</div> <a itemprop="sameAs" href="http://www.ncbi.nlm.nih.gov/pubmed/25058401"> <div itemprop="name">A biomaterial model of tumor stromal microenvironment promotes mesenchymal morphology but not epithelial to mesenchymal transition in epithelial cells.</div> </a> <div itemprop="about">Acta biomaterialia</div> <div itemprop="author">McLane JS,Rivet CJ,Gilbert RJ,Ligon LA</div> <div itemprop="description">MA5-15065 was used in western blot to describe a new in vitro model to test the effect of an aligned fibrous environment on cancer cell morphology and behavior</div> <div itemprodp="datePublished">Sat Nov 01 00:00:00 EDT 2014</div> </div> </noscript> </div> <div class="border-bottom"></div> <div class="product-bioinformatics-details"> <div class="container"> <h2 class="pdp-sub-heading">Bioinformatics</h2> <div class="row"> <div class="large-span-6 medium-span-12 small-span-12"> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="proteinContent"> <strong>Protein Aliases:</strong> latency-associated peptide; prepro-transforming growth factor beta-1; TGF b; TGF b 1; TGF beta; tgf beta 1; TGF beta-1; TGF beta-1 protein; tgf beta1; TGF β; TGF β 1; TGF β1; TGF-B1; TGF-beta 1 protein; TGF-beta-1; TGFβ1; Transforming growth factor; transforming growth factor b1; transforming growth factor beta; Transforming growth factor beta-1 proprotein; transforming growth factor β1 </p> <p class="view-buttons" id="ptnViewBtn" ng-class="$ctrl.bioContentLines('proteinContent', 'ptnViewBtn')"> <a id="proteinViewMoreBtn" ng-click="$ctrl.bioContentViewMore('proteinViewMoreBtn', 'proteinViewLessBtn', 'proteinContent')"> View more </a> <a id="proteinViewLessBtn" ng-click="$ctrl.bioContentViewLess('proteinViewLessBtn', 'proteinViewMoreBtn', 'proteinContent')"> View less </a> </p> </div> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="geneContent"> <strong>Gene Aliases:</strong> CED; DPD1; LAP; TGFB; TGFB1; TGFbeta </p> <p class="view-buttons" id="geneViewBtn" ng-class="$ctrl.bioContentLines('geneContent', 'geneViewBtn')"> <a id="geneViewMoreBtn" ng-click="$ctrl.bioContentViewMore('geneViewMoreBtn', 'geneViewLessBtn', 'geneContent')"> View more </a> <a id="geneViewLessBtn" ng-click="$ctrl.bioContentViewLess('geneViewLessBtn', 'geneViewMoreBtn', 'geneContent')"> View less </a> </p> </div> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="unitProContent"> <strong>UniProt ID:</strong> <a href="http://www.uniprot.org/uniprot/P01137" target="_blank">(Human) P01137</a> </p> <p class="view-buttons" id="unitViewBtn" ng-class="$ctrl.bioContentLines('unitProContent', 'unitViewBtn')"> <a id="unitProViewMoreBtn" ng-click="$ctrl.bioContentViewMore('unitProViewMoreBtn', 'unitProViewLessBtn', 'unitProContent')"> View more </a> <a id="unitProViewLessBtn" ng-click="$ctrl.bioContentViewLess('unitProViewLessBtn', 'unitProViewMoreBtn', 'unitProContent')"> View less </a> </p> </div> <div class="bioinformatics-wrapper"> <p class="bioinformatics-detail-section" id="entrezContent"> <strong>Entrez Gene ID:</strong> <a href="http://www.ncbi.nlm.nih.gov/gene?term=7040" target="_blank">(Human) 7040</a> </p> <p class="view-buttons" id="etzViewBtn" ng-class="$ctrl.bioContentLines('entrezContent', 'etzViewBtn')"> <a id="entrezViewMoreBtn" ng-click="$ctrl.bioContentViewMore('entrezViewMoreBtn', 'entrezViewLessBtn', 'entrezContent')"> View more </a> <a id="entrezViewLessBtn" ng-click="$ctrl.bioContentViewLess('entrezViewLessBtn', 'entrezViewMoreBtn', 'entrezContent')"> View less </a> </p> </div> </div> <div class="large-span-6 medium-span-12 small-span-12"> <div class="accordion" id="accordion-bioinformatics"> <div class="accordion-group"> <div class="accordion-heading"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion2" target="_self" href="#collapseOne"> Function(s) </a> </div> <div id="collapseOne" class="accordion-body collapse"> <div class="accordion-inner"> <a class="icon" href="/antibody/primary/panther/glycoprotein binding"> glycoprotein binding </a> <a class="icon" href="/antibody/primary/panther/antigen binding"> antigen binding </a> <a class="icon" href="/antibody/primary/panther/type II transforming growth factor beta receptor binding"> type II transforming growth factor beta receptor binding </a> <a class="icon" href="/antibody/primary/panther/cytokine activity"> cytokine activity </a> <a class="icon" href="/antibody/primary/panther/transforming growth factor beta receptor binding"> transforming growth factor beta receptor binding </a> <a class="icon" href="/antibody/primary/panther/protein binding"> protein binding </a> <a class="icon" href="/antibody/primary/panther/growth factor activity"> growth factor activity </a> <a class="icon" href="/antibody/primary/panther/enzyme binding"> enzyme binding </a> <a class="icon" href="/antibody/primary/panther/type I transforming growth factor beta receptor binding"> type I transforming growth factor beta receptor binding </a> <a class="icon" href="/antibody/primary/panther/type III transforming growth factor beta receptor binding"> type III transforming growth factor beta receptor binding </a> <a class="icon" href="/antibody/primary/panther/protein homodimerization activity"> protein homodimerization activity </a> <a class="icon" href="/antibody/primary/panther/protein serine/threonine kinase activator activity"> protein serine/threonine kinase activator activity </a> <a class="icon" href="/antibody/primary/panther/protein heterodimerization activity"> protein heterodimerization activity </a> <a class="icon" href="/antibody/primary/panther/protein N-terminus binding"> protein N-terminus binding </a> <a class="icon" href="/antibody/primary/panther/growth factor"> growth factor </a> </div> </div> </div> <div class="accordion-group"> <div class="accordion-heading"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion2" target="_self" href="#collapseTwo"> Process(es) </a> </div> <div id="collapseTwo" class="accordion-body collapse"> <div class="accordion-inner"> <a class="icon" href="/antibody/primary/panther/protein import into nucleus, translocation"> protein import into nucleus, translocation </a> <a class="icon" href="/antibody/primary/panther/negative regulation of transcription from RNA polymerase II promoter"> negative regulation of transcription from RNA polymerase II promoter </a> <a class="icon" href="/antibody/primary/panther/MAPK cascade"> MAPK cascade </a> <a class="icon" href="/antibody/primary/panther/ureteric bud development"> ureteric bud development </a> <a class="icon" href="/antibody/primary/panther/response to hypoxia"> response to hypoxia </a> <a class="icon" href="/antibody/primary/panther/epithelial to mesenchymal transition"> epithelial to mesenchymal transition </a> <a class="icon" href="/antibody/primary/panther/negative regulation of protein phosphorylation"> negative regulation of protein phosphorylation </a> <a class="icon" href="/antibody/primary/panther/positive regulation of protein phosphorylation"> positive regulation of protein phosphorylation </a> <a class="icon" href="/antibody/primary/panther/regulation of sodium ion transport"> regulation of sodium ion transport </a> <a class="icon" href="/antibody/primary/panther/chondrocyte differentiation"> chondrocyte differentiation </a> <a class="icon" href="/antibody/primary/panther/hematopoietic progenitor cell differentiation"> hematopoietic progenitor cell differentiation </a> <a class="icon" href="/antibody/primary/panther/connective tissue replacement involved in inflammatory response wound healing"> connective tissue replacement involved in inflammatory response wound healing </a> <a class="icon" href="/antibody/primary/panther/adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains"> adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains </a> <a class="icon" href="/antibody/primary/panther/tolerance induction to self antigen"> tolerance induction to self antigen </a> <a class="icon" href="/antibody/primary/panther/platelet degranulation"> platelet degranulation </a> <a class="icon" href="/antibody/primary/panther/protein phosphorylation"> protein phosphorylation </a> <a class="icon" href="/antibody/primary/panther/protein export from nucleus"> protein export from nucleus </a> <a class="icon" href="/antibody/primary/panther/ATP biosynthetic process"> ATP biosynthetic process </a> <a class="icon" href="/antibody/primary/panther/phosphate-containing compound metabolic process"> phosphate-containing compound metabolic process </a> <a class="icon" href="/antibody/primary/panther/cellular calcium ion homeostasis"> cellular calcium ion homeostasis </a> <a class="icon" href="/antibody/primary/panther/inflammatory response"> inflammatory response </a> <a class="icon" href="/antibody/primary/panther/cell cycle arrest"> cell cycle arrest </a> <a class="icon" href="/antibody/primary/panther/mitotic cell cycle checkpoint"> mitotic cell cycle checkpoint </a> <a class="icon" href="/antibody/primary/panther/epidermal growth factor receptor signaling pathway"> epidermal growth factor receptor signaling pathway </a> <a class="icon" href="/antibody/primary/panther/transforming growth factor beta receptor signaling pathway"> transforming growth factor beta receptor signaling pathway </a> <a class="icon" href="/antibody/primary/panther/common-partner SMAD protein phosphorylation"> common-partner SMAD protein phosphorylation </a> <a class="icon" href="/antibody/primary/panther/SMAD protein complex assembly"> SMAD protein complex assembly </a> <a class="icon" href="/antibody/primary/panther/SMAD protein import into nucleus"> SMAD protein import into nucleus </a> <a class="icon" href="/antibody/primary/panther/Notch signaling pathway"> Notch signaling pathway </a> <a class="icon" href="/antibody/primary/panther/negative regulation of neuroblast proliferation"> negative regulation of neuroblast proliferation </a> <a class="icon" href="/antibody/primary/panther/salivary gland morphogenesis"> salivary gland morphogenesis </a> <a class="icon" href="/antibody/primary/panther/endoderm development"> endoderm development </a> <a class="icon" href="/antibody/primary/panther/female pregnancy"> female pregnancy </a> <a class="icon" href="/antibody/primary/panther/aging"> aging </a> <a class="icon" href="/antibody/primary/panther/negative regulation of DNA replication"> negative regulation of DNA replication </a> <a class="icon" href="/antibody/primary/panther/positive regulation of cell proliferation"> positive regulation of cell proliferation </a> <a class="icon" href="/antibody/primary/panther/negative regulation of cell proliferation"> negative regulation of cell proliferation </a> <a class="icon" href="/antibody/primary/panther/germ cell migration"> germ cell migration </a> <a class="icon" href="/antibody/primary/panther/response to radiation"> response to radiation </a> <a class="icon" href="/antibody/primary/panther/response to wounding"> response to wounding </a> <a class="icon" href="/antibody/primary/panther/response to glucose"> response to glucose </a> <a class="icon" href="/antibody/primary/panther/defense response to fungus, incompatible interaction"> defense response to fungus, incompatible interaction </a> <a class="icon" href="/antibody/primary/panther/positive regulation of vascular endothelial growth factor production"> positive regulation of vascular endothelial growth factor production </a> <a class="icon" href="/antibody/primary/panther/positive regulation of gene expression"> positive regulation of gene expression </a> <a class="icon" href="/antibody/primary/panther/negative regulation of gene expression"> negative regulation of gene expression </a> <a class="icon" href="/antibody/primary/panther/negative regulation of extracellular matrix disassembly"> negative regulation of extracellular matrix disassembly </a> <a class="icon" href="/antibody/primary/panther/positive regulation of epithelial to mesenchymal transition"> positive regulation of epithelial to mesenchymal transition </a> <a class="icon" href="/antibody/primary/panther/macrophage derived foam cell differentiation"> macrophage derived foam cell differentiation </a> <a class="icon" href="/antibody/primary/panther/positive regulation of fibroblast migration"> positive regulation of fibroblast migration </a> <a class="icon" href="/antibody/primary/panther/positive regulation of peptidyl-threonine phosphorylation"> positive regulation of peptidyl-threonine phosphorylation </a> <a class="icon" href="/antibody/primary/panther/positive regulation of pathway-restricted SMAD protein phosphorylation"> positive regulation of pathway-restricted SMAD protein phosphorylation </a> <a class="icon" href="/antibody/primary/panther/negative regulation of macrophage cytokine production"> negative regulation of macrophage cytokine production </a> <a class="icon" href="/antibody/primary/panther/oligodendrocyte development"> oligodendrocyte development </a> <a class="icon" href="/antibody/primary/panther/cell growth"> cell growth </a> <a class="icon" href="/antibody/primary/panther/regulation of striated muscle tissue development"> regulation of striated muscle tissue development </a> <a class="icon" href="/antibody/primary/panther/cell migration"> cell migration </a> <a class="icon" href="/antibody/primary/panther/regulation of transforming growth factor beta receptor signaling pathway"> regulation of transforming growth factor beta receptor signaling pathway </a> <a class="icon" href="/antibody/primary/panther/evasion or tolerance of host defenses by virus"> evasion or tolerance of host defenses by virus </a> <a class="icon" href="/antibody/primary/panther/negative regulation of cell-cell adhesion"> negative regulation of cell-cell adhesion </a> <a class="icon" href="/antibody/primary/panther/hyaluronan catabolic process"> hyaluronan catabolic process </a> <a class="icon" href="/antibody/primary/panther/negative regulation of ossification"> negative regulation of ossification </a> <a class="icon" href="/antibody/primary/panther/negative regulation of cell growth"> negative regulation of cell growth </a> <a class="icon" href="/antibody/primary/panther/regulation of cell migration"> regulation of cell migration </a> <a class="icon" href="/antibody/primary/panther/positive regulation of cell migration"> positive regulation of cell migration </a> <a class="icon" href="/antibody/primary/panther/positive regulation of bone mineralization"> positive regulation of bone mineralization </a> <a class="icon" href="/antibody/primary/panther/negative regulation of transforming growth factor beta receptor signaling pathway"> negative regulation of transforming growth factor beta receptor signaling pathway </a> <a class="icon" href="/antibody/primary/panther/positive regulation of histone deacetylation"> positive regulation of histone deacetylation </a> <a class="icon" href="/antibody/primary/panther/organ regeneration"> organ regeneration </a> <a class="icon" href="/antibody/primary/panther/positive regulation of protein complex assembly"> positive regulation of protein complex assembly </a> <a class="icon" href="/antibody/primary/panther/positive regulation of exit from mitosis"> positive regulation of exit from mitosis </a> <a class="icon" href="/antibody/primary/panther/lipopolysaccharide-mediated signaling pathway"> lipopolysaccharide-mediated signaling pathway </a> <a class="icon" href="/antibody/primary/panther/positive regulation of cellular protein metabolic process"> positive regulation of cellular protein metabolic process </a> <a class="icon" href="/antibody/primary/panther/response to estradiol"> response to estradiol </a> <a class="icon" href="/antibody/primary/panther/response to progesterone"> response to progesterone </a> <a class="icon" href="/antibody/primary/panther/regulation of interleukin-23 production"> regulation of interleukin-23 production </a> <a class="icon" href="/antibody/primary/panther/negative regulation of interleukin-17 production"> negative regulation of interleukin-17 production </a> <a class="icon" href="/antibody/primary/panther/positive regulation of interleukin-17 production"> positive regulation of interleukin-17 production </a> <a class="icon" href="/antibody/primary/panther/receptor catabolic process"> receptor catabolic process </a> <a class="icon" href="/antibody/primary/panther/positive regulation of superoxide anion generation"> positive regulation of superoxide anion generation </a> <a class="icon" href="/antibody/primary/panther/mononuclear cell proliferation"> mononuclear cell proliferation </a> <a class="icon" href="/antibody/primary/panther/positive regulation of collagen biosynthetic process"> positive regulation of collagen biosynthetic process </a> <a class="icon" href="/antibody/primary/panther/positive regulation of peptidyl-serine phosphorylation"> positive regulation of peptidyl-serine phosphorylation </a> <a class="icon" href="/antibody/primary/panther/response to vitamin D"> response to vitamin D </a> <a class="icon" href="/antibody/primary/panther/response to laminar fluid shear stress"> response to laminar fluid shear stress </a> <a class="icon" href="/antibody/primary/panther/positive regulation of histone acetylation"> positive regulation of histone acetylation </a> <a class="icon" href="/antibody/primary/panther/positive regulation of protein dephosphorylation"> positive regulation of protein dephosphorylation </a> <a class="icon" href="/antibody/primary/panther/negative regulation of T cell proliferation"> negative regulation of T cell proliferation </a> <a class="icon" href="/antibody/primary/panther/regulation of protein import into nucleus"> regulation of protein import into nucleus </a> <a class="icon" href="/antibody/primary/panther/positive regulation of protein import into nucleus"> positive regulation of protein import into nucleus </a> <a class="icon" href="/antibody/primary/panther/positive regulation of odontogenesis"> positive regulation of odontogenesis </a> <a class="icon" href="/antibody/primary/panther/response to drug"> response to drug </a> <a class="icon" href="/antibody/primary/panther/myelination"> myelination </a> <a class="icon" href="/antibody/primary/panther/regulation of apoptotic process"> regulation of apoptotic process </a> <a class="icon" href="/antibody/primary/panther/myeloid dendritic cell differentiation"> myeloid dendritic cell differentiation </a> <a class="icon" href="/antibody/primary/panther/T cell homeostasis"> T cell homeostasis </a> <a class="icon" href="/antibody/primary/panther/positive regulation of apoptotic process"> positive regulation of apoptotic process </a> <a class="icon" href="/antibody/primary/panther/positive regulation of vascular permeability"> positive regulation of vascular permeability </a> <a class="icon" href="/antibody/primary/panther/positive regulation of MAP kinase activity"> positive regulation of MAP kinase activity </a> <a class="icon" href="/antibody/primary/panther/protein kinase B signaling"> protein kinase B signaling </a> <a class="icon" href="/antibody/primary/panther/positive regulation of blood vessel endothelial cell migration"> positive regulation of blood vessel endothelial cell migration </a> <a class="icon" href="/antibody/primary/panther/negative regulation of blood vessel endothelial cell migration"> negative regulation of blood vessel endothelial cell migration </a> <a class="icon" href="/antibody/primary/panther/positive regulation of phosphatidylinositol 3-kinase activity"> positive regulation of phosphatidylinositol 3-kinase activity </a> <a class="icon" href="/antibody/primary/panther/ossification involved in bone remodeling"> ossification involved in bone remodeling </a> <a class="icon" href="/antibody/primary/panther/regulatory T cell differentiation"> regulatory T cell differentiation </a> <a class="icon" href="/antibody/primary/panther/cell-cell junction organization"> cell-cell junction organization </a> <a class="icon" href="/antibody/primary/panther/positive regulation of regulatory T cell differentiation"> positive regulation of regulatory T cell differentiation </a> <a class="icon" href="/antibody/primary/panther/negative regulation of cell differentiation"> negative regulation of cell differentiation </a> <a class="icon" href="/antibody/primary/panther/negative regulation of fat cell differentiation"> negative regulation of fat cell differentiation </a> <a class="icon" href="/antibody/primary/panther/negative regulation of myoblast differentiation"> negative regulation of myoblast differentiation </a> <a class="icon" href="/antibody/primary/panther/negative regulation of cell cycle"> negative regulation of cell cycle </a> <a class="icon" href="/antibody/primary/panther/negative regulation of transcription, DNA-templated"> negative regulation of transcription, DNA-templated </a> <a class="icon" href="/antibody/primary/panther/positive regulation of transcription, DNA-templated"> positive regulation of transcription, DNA-templated </a> <a class="icon" href="/antibody/primary/panther/negative regulation of mitotic cell cycle"> negative regulation of mitotic cell cycle </a> <a class="icon" href="/antibody/primary/panther/positive regulation of transcription from RNA polymerase II promoter"> positive regulation of transcription from RNA polymerase II promoter </a> <a class="icon" href="/antibody/primary/panther/active induction of host immune response by virus"> active induction of host immune response by virus </a> <a class="icon" href="/antibody/primary/panther/positive regulation of fibroblast proliferation"> positive regulation of fibroblast proliferation </a> <a class="icon" href="/antibody/primary/panther/positive regulation of isotype switching to IgA isotypes"> positive regulation of isotype switching to IgA isotypes </a> <a class="icon" href="/antibody/primary/panther/lymph node development"> lymph node development </a> <a class="icon" href="/antibody/primary/panther/digestive tract development"> digestive tract development </a> <a class="icon" href="/antibody/primary/panther/negative regulation of skeletal muscle tissue development"> negative regulation of skeletal muscle tissue development </a> <a class="icon" href="/antibody/primary/panther/inner ear development"> inner ear development </a> <a class="icon" href="/antibody/primary/panther/positive regulation of epithelial cell proliferation"> positive regulation of epithelial cell proliferation </a> <a class="icon" href="/antibody/primary/panther/negative regulation of epithelial cell proliferation"> negative regulation of epithelial cell proliferation </a> <a class="icon" href="/antibody/primary/panther/positive regulation of protein secretion"> positive regulation of protein secretion </a> <a class="icon" href="/antibody/primary/panther/positive regulation of peptidyl-tyrosine phosphorylation"> positive regulation of peptidyl-tyrosine phosphorylation </a> <a class="icon" href="/antibody/primary/panther/negative regulation of phagocytosis"> negative regulation of phagocytosis </a> <a class="icon" href="/antibody/primary/panther/positive regulation of chemotaxis"> positive regulation of chemotaxis </a> <a class="icon" href="/antibody/primary/panther/positive regulation of NF-kappaB transcription factor activity"> positive regulation of NF-kappaB transcription factor activity </a> <a class="icon" href="/antibody/primary/panther/regulation of binding"> regulation of binding </a> <a class="icon" href="/antibody/primary/panther/regulation of DNA binding"> regulation of DNA binding </a> <a class="icon" href="/antibody/primary/panther/positive regulation of smooth muscle cell differentiation"> positive regulation of smooth muscle cell differentiation </a> <a class="icon" href="/antibody/primary/panther/negative regulation of release of sequestered calcium ion into cytosol"> negative regulation of release of sequestered calcium ion into cytosol </a> <a class="icon" href="/antibody/primary/panther/positive regulation of cell division"> positive regulation of cell division </a> <a class="icon" href="/antibody/primary/panther/positive regulation of protein kinase B signaling"> positive regulation of protein kinase B signaling </a> <a class="icon" href="/antibody/primary/panther/regulation of blood vessel remodeling"> regulation of blood vessel remodeling </a> <a class="icon" href="/antibody/primary/panther/face morphogenesis"> face morphogenesis </a> <a class="icon" href="/antibody/primary/panther/frontal suture morphogenesis"> frontal suture morphogenesis </a> <a class="icon" href="/antibody/primary/panther/pathway-restricted SMAD protein phosphorylation"> pathway-restricted SMAD protein phosphorylation </a> <a class="icon" href="/antibody/primary/panther/regulation of SMAD protein import into nucleus"> regulation of SMAD protein import into nucleus </a> <a class="icon" href="/antibody/primary/panther/positive regulation of SMAD protein import into nucleus"> positive regulation of SMAD protein import into nucleus </a> <a class="icon" href="/antibody/primary/panther/SMAD protein signal transduction"> SMAD protein signal transduction </a> <a class="icon" href="/antibody/primary/panther/mammary gland branching involved in thelarche"> mammary gland branching involved in thelarche </a> <a class="icon" href="/antibody/primary/panther/branch elongation involved in mammary gland duct branching"> branch elongation involved in mammary gland duct branching </a> <a class="icon" href="/antibody/primary/panther/regulation of branching involved in mammary gland duct morphogenesis"> regulation of branching involved in mammary gland duct morphogenesis </a> <a class="icon" href="/antibody/primary/panther/negative regulation of gene silencing by miRNA"> negative regulation of gene silencing by miRNA </a> <a class="icon" href="/antibody/primary/panther/regulation of cartilage development"> regulation of cartilage development </a> <a class="icon" href="/antibody/primary/panther/lens fiber cell differentiation"> lens fiber cell differentiation </a> <a class="icon" href="/antibody/primary/panther/response to cholesterol"> response to cholesterol </a> <a class="icon" href="/antibody/primary/panther/positive regulation of cell cycle arrest"> positive regulation of cell cycle arrest </a> <a class="icon" href="/antibody/primary/panther/cellular response to organic cyclic compound"> cellular response to organic cyclic compound </a> <a class="icon" href="/antibody/primary/panther/cellular response to dexamethasone stimulus"> cellular response to dexamethasone stimulus </a> <a class="icon" href="/antibody/primary/panther/cellular response to transforming growth factor beta stimulus"> cellular response to transforming growth factor beta stimulus </a> <a class="icon" href="/antibody/primary/panther/extracellular matrix assembly"> extracellular matrix assembly </a> <a class="icon" href="/antibody/primary/panther/positive regulation of branching involved in ureteric bud morphogenesis"> positive regulation of branching involved in ureteric bud morphogenesis </a> <a class="icon" href="/antibody/primary/panther/extrinsic apoptotic signaling pathway"> extrinsic apoptotic signaling pathway </a> <a class="icon" href="/antibody/primary/panther/negative regulation of hyaluronan biosynthetic process"> negative regulation of hyaluronan biosynthetic process </a> <a class="icon" href="/antibody/primary/panther/positive regulation of extracellular matrix assembly"> positive regulation of extracellular matrix assembly </a> <a class="icon" href="/antibody/primary/panther/positive regulation of NAD+ ADP-ribosyltransferase activity"> positive regulation of NAD+ ADP-ribosyltransferase activity </a> <a class="icon" href="/antibody/primary/panther/positive regulation of pri-miRNA transcription from RNA polymerase II promoter"> positive regulation of pri-miRNA transcription from RNA polymerase II promoter </a> <a class="icon" href="/antibody/primary/panther/negative regulation of protein localization to plasma membrane"> negative regulation of protein localization to plasma membrane </a> <a class="icon" href="/antibody/primary/panther/negative regulation of production of miRNAs involved in gene silencing by miRNA"> negative regulation of production of miRNAs involved in gene silencing by miRNA </a> <a class="icon" href="/antibody/primary/panther/positive regulation of receptor clustering"> positive regulation of receptor clustering </a> <a class="icon" href="/antibody/primary/panther/regulation of epithelial to mesenchymal transition involved in endocardial cushion formation"> regulation of epithelial to mesenchymal transition involved in endocardial cushion formation </a> <a class="icon" href="/antibody/primary/panther/regulation of actin cytoskeleton reorganization"> regulation of actin cytoskeleton reorganization </a> <a class="icon" href="/antibody/primary/panther/positive regulation of transcription regulatory region DNA binding"> positive regulation of transcription regulatory region DNA binding </a> <a class="icon" href="/antibody/primary/panther/positive regulation of cardiac muscle cell differentiation"> positive regulation of cardiac muscle cell differentiation </a> </div> </div> </div> </div> </div> </div> </div> </div> <div class="recommended-products 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