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<!doctype html> <html lang="de"> <head> <title>Den Arbeitsablauf der Probenvorbereitung verstehen: Halbauto | 1099778</title> <meta name="keywords" content="Ramisa Fariha, Collin Hill, Mohannad Jabrah, Adam Spooner, Anubhav Tripathi* , "/> <meta name="description" content="High-performance liquid chromatography-tandem mass spectrometry has been pivotal to recent clinical advancements in the detection and monitoring of the pro..1099778"/> <meta name="citation_publisher" content="Longdom Publishing SL" /> <meta name="citation_journal_title" content="Andrologie- Offener Zugang"> <meta name="citation_title" content="Den Arbeitsablauf der Probenvorbereitung verstehen: Halbautomatische Vorbereitung von Serumtestosteron zur Erkennung mittels LC-MS/MS "> <meta name="citation_author" content="Ramisa Fariha" /> <meta name="citation_author" content="Collin Hill" /> <meta name="citation_author" content="Mohannad Jabrah" /> <meta name="citation_author" content="Adam Spooner" /> <meta name="citation_author" content="Anubhav Tripathi" /> <meta name="citation_year" content=""> <meta name="citation_volume" content="13"> <meta name="citation_issue" content="5"> <meta name="citation_abstract" content="High-performance liquid chromatography-tandem mass spectrometry has been pivotal to recent clinical advancements in the detection and monitoring of the progression of different metabolic disorders. Such systems have been used to routinely monitor serum testosterone levels in patients, to both mitigate side effects of different medications, as well as diagnose androgen-associated disorders. The present study develops and validates an LC-MS/MS-based assay for the detection of total serum testosterone from only (100 μL) of sample volume. To increase the high throughput capability of the developed diagnostic kit for clinical use on patient samples, the proposed method was streamlined via semi-automation on a JANUS® G3 Workstation liquid handler. The proposed diagnostic kit showcased high detection precision (CV<5%) as well as sample accuracy (93%-108%), within a clinically relevant range of testosterone serum concentrations (5-1500 ng/dl). Moreover, the assay showcased LOD of 5 ng/dl, which is significantly comparable to commercially established diagnostic tools. The developed method also exhibited high linearity over the specified testosterone serum range, with an obtained linearity of R2>0.99 using both manual as well as automated sample preparation. Overall, the work shown presents an optimized, automatable LC-MS/MSbased testosterone diagnostic assay for the routine evaluation of patient testosterone levels in serum for clinical and research use. 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Such systems have been used to routinely monitor serum testosterone levels in patients, to both mitigate side effects of different medications, as well as diagnose androgen-associated disorders. The present study develops and validates an LC-MS/MS-based assay for the detection of total serum testosterone from only (100 μL) of sample volume. To increase the high throughput capability of the developed diagnostic kit for clinical use on patient samples, the proposed method was streamlined via semi-automation on a JANUS<sup>® </sup>G3 Workstation liquid handler. The proposed diagnostic kit showcased high detection precision (CV<5%) as well as sample accuracy (93%-108%), within a clinically relevant range of testosterone serum concentrations (5-1500 ng/dl). Moreover, the assay showcased LOD of 5 ng/dl, which is significantly comparable to commercially established diagnostic tools. The developed method also exhibited high linearity over the specified testosterone serum range, with an obtained linearity of R2>0.99 using both manual as well as automated sample preparation. Overall, the work shown presents an optimized, automatable LC-MS/MSbased testosterone diagnostic assay for the routine evaluation of patient testosterone levels in serum for clinical and research use.</p> <div class="alert alert-info text-left"><b>Haftungsausschluss:</b> Diese Zusammenfassung wurde mithilfe von Tools der k眉nstlichen Intelligenz 眉bersetzt und wurde noch nicht 眉berpr眉ft oder verifiziert.</div> <div class="nav social-icons"> <a class="nav-link w-auto">Teile diesen Artikel</a> <a title="Hier klicken" target="_blank" href="https://www.facebook.com/sharer.php?u=https://german.longdom.org/abstract/understanding-sample-preparation-workflow-semiautomated-preparation-of-serum-testosterone-for-detection-using-lcmsms-1099778.html" rel="noopener"><i class="fab fa-facebook-f"></i></a> <a class="nav-link" title="Hier klicken" target="_blank" href="https://twitter.com/share?url=https://german.longdom.org/abstract/understanding-sample-preparation-workflow-semiautomated-preparation-of-serum-testosterone-for-detection-using-lcmsms-1099778.html" rel="noopener"><i class="fab fa-twitter"></i></a> <a class="nav-link" title="Hier klicken" target="_blank" href="https://www.linkedin.com/shareArticle?mini=true&url=https://german.longdom.org/abstract/understanding-sample-preparation-workflow-semiautomated-preparation-of-serum-testosterone-for-detection-using-lcmsms-1099778.html" rel="noopener"><i class="fab fa-linkedin-in"></i></a> <a class="nav-link" title="Hier klicken" target="_blank" href="https://plus.google.com/share?url=https://german.longdom.org/abstract/understanding-sample-preparation-workflow-semiautomated-preparation-of-serum-testosterone-for-detection-using-lcmsms-1099778.html" rel="noopener"><i class="fab fa-google-plus-g"></i></a> </div> </div> </div> </div> </section> <footer class="bg-blue-grey-900 py-3"> <div class="container"> <div class="row"> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">Inhaltslinks</h4> <ul class="list-unstyled footer-links font-size-3"> <li><a class="" href="https://german.longdom.org/privacy-policy.html" title="Hier klicken">Datenschutzrichtlinie</a></li> <li><a class="" href="https://german.longdom.org/terms-conditions.html" title="Hier klicken">Allgemeine Gesch盲ftsbedingungen</a></li> <li><a class="" href="https://german.longdom.org/authors-reviewers-editors.html" title="Hier klicken">Autoren, Gutachter & Herausgeber</a></li> </ul> </div> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">Kontaktieren Sie Longdom</h4> <p><strong>Longdom Group SA</strong><br /> Avenue Roger Vandendriessche,<br /> 18, 1150 Br眉ssel, Belgien<br /> Telefon: +442038085340<br /> <strong>E-Mail:</strong> <a class="white" href="mailto:info@longdom.org" title="Hier klicken">info@longdom.org</a></p> </div> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">Verbinden</h4> <nav class="nav nav-pills social-icons-footer flex-column a-pl-0"> <a href="https://www.facebook.com/longdompublisher" title="Hier klicken" target="_blank" class="nav-link bg-facebook-hover"><i class="fab fa-facebook-f bg-facebook"></i></a> <a href="https://www.linkedin.com/company/longdom-publishing-sl/" title="Hier klicken" target="_blank" class="nav-link bg-linkedin-hover"><i class="fab fa-linkedin-in bg-linkedin"></i></a> <a href="https://twitter.com/LongdomP" title="Hier klicken" target="_blank" class="nav-link bg-twitter-hover"><i class="fab fa-twitter bg-twitter"></i></a> <a href="https://www.instagram.com/longdom_publisher/" title="Hier klicken" target="_blank" class="nav-link bg-instagram-hover"><i class="fab fa-instagram bg-instagram"></i></a> </nav> </div> </div> <div class="row text-center"> <div class="col"> <p>Urheberrechte 漏 © 2025 <a href="https://german.longdom.org/" title="Hier klicken" class="white">Longdom Publishing SL</a>.</p> </div> </div> </div> </footer>  <a href="#0" class="cd-top js-cd-top">Top</a>   <script src="https://code.jquery.com/jquery-3.3.1.min.js"></script> <script src="https://cdnjs.cloudflare.com/ajax/libs/popper.js/1.14.7/umd/popper.min.js"></script> <script src="https://stackpath.bootstrapcdn.com/bootstrap/4.3.1/js/bootstrap.min.js"></script>  <script> jQuery(function($) { $(window).scroll(function fix_element() { $('#target').css( $(window).scrollTop() > 100 ? 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