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Sequencing-induced artefacts in NGS STR data - Peeref
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</nav> <main> <div id="top-info-banner" class="container-fluid mb-0"> <div class="container"> <div class="d-flex align-items-center" style="margin-top: 30px;"> <span class="text-white"> <strong class="f18">☆</strong> <span class="f16">4.5</span> </span> <span class="mx-3"></span> <span class="tag">Article</span> </div> <h1 class="title title-for-article"> Sequencing-induced artefacts in NGS STR data </h1> <div class="help-links-left"> <p class="pub-info"> FORENSIC SCIENCE INTERNATIONAL-GENETICS (2024) </p> </div> </div> </div> <div id="article-sticky-navbar"> <div class="container"> <div class="d-flex justify-content-between flex-wrap flex-md-nowrap"> <div class="d-flex align-items-center mb-2"> <ul class="nav nav-underline f16 font-weight-bold"> <li class="active"> <a href="javascript:;"> Overview </a> </li> <li class=""> <a href="https://www.peeref.com/works/83094956/comments"> Write a Review </a> </li> </ul> </div> <div class="d-flex align-items-center justify-content-md-end 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class="my-2"> DOI: 10.1016/j.fsigen.2024.103086 </div> </div> </div> <div class="mb-3 pb-3"> <h4 class="mt-0">Keywords</h4> <div class="f16"> Forensic genetics; STRs; MiSeq FGx; Sequencing artifacts; Noise spikes; Quality scores </div> </div> <div class="mb-3 pb-3"> <h4 class="mt-0">Categories</h4> <div class="f16"> <span class="d-block"> <a href="https://www.peeref.com/works/list?category=Genetics+%26+Heredity" target="_blank" class="text-dark btn btn-link p-0 text-left"> Genetics & Heredity </a> </span> <span class="d-block"> <a href="https://www.peeref.com/works/list?category=Medicine%2C+Legal" target="_blank" class="text-dark btn btn-link p-0 text-left"> Medicine, Legal </a> </span> </div> </div> <div class="mb-3 pb-3"> <h4 class="mt-0">Funding</h4> <div class="f16"> <ol class=""> <li>Department of Homeland Security (DHS) Science and Technology Directorate [HSHQDC-15-C- 00064]</li> <li>Research and Development Center</li> <li>Battelle National Biodefense Institute, LLC [HSHQDC-15-C-00064]</li> </ol> </div> </div> </div> <div class="f15 panel-box rounded shadow-none border"> <h4 class="mt-0 text-center">Ask authors/readers for more resources</h4> <div class="requests"> <div class="requests-item"> <div class="icon"> <img src="https://peeref-open.s3.amazonaws.com/images/file.png" alt=""> </div> <h4>Protocol</h4> <p> <a href="https://www.peeref.com/works/83094956/resource" class="btn btn-outline-primary btn-sm"> Community support </a> </p> </div> <div class="requests-item"> <div class="icon"> <img src="https://peeref-open.s3.amazonaws.com/images/experiment.png" alt=""> </div> <h4>Reagent</h4> <p> <a href="https://www.peeref.com/works/83094956/resource" class="btn btn-outline-primary btn-sm"> Community support </a> </p> </div> </div> </div> </div> <div class="col-md-8 px-0 pl-md-3"> <div id="article-summary-panel" class="mb-4"> <ul class="nav nav-tabs" style="list-style: none; padding-left: 0;"> <li class="active"> <a href="#ai_summary" data-toggle="tab" class="summary-tab mx-0 f16 text-dark"> <strong>Automated Summary</strong> <strong class="text-danger ml-1"><i>New</i></strong> </a> </li> <li class=""> <a href="#raw_abstract" data-toggle="tab" class="abstract-tab mx-0 f16 text-dark"> <strong>Abstract</strong> </a> </li> </ul> <div class="tab-content border border-top-0"> <div id="ai_summary" class="tab-pane active"> <div class="summary-panel panel-box mb-0 rounded shadow-none"> <div class="f16">This paper mainly introduces the high-read count noise sequences observed in the analysis of DNA profiles using the MiSeq FGx sequencing system, as well as the characteristics and causes of these noise sequences. The authors also introduce the methods used to detect these noise sequences and the help these methods provide to the laboratory.</div> </div> </div> <div id="raw_abstract" class="tab-pane "> <div class="abstract-panel panel-box mb-0 rounded shadow-none"> <div class="f16">Significant progress has been made in recent years in the development of techniques for Next Generation Sequencing (NGS), or Massively Parallel Sequencing (MPS), of forensically relevant short tandem repeat (STR) loci. However, as these technologies are investigated and adopted by forensic laboratories, new challenges unfold that require further scrutiny. In the analysis of DNA profiles generated using the MiSeq FGx sequencing system, we have observed noise sequences with relatively high readcounts that are challenging to distinguish from genuine alleles. These high read count noise sequences appear as allele sequences with one or a few substituted bases compared to a known allele sequence within the profile. An examination of ForenSeq DNA Signature Prep Kit STR noise sequences revealed that the substituted base of a parent allele can align to the same position on the sequence across noise sequences. This suggests that these substitution events occur at specific positions within the amplicon, resulting in multiple noise reads with substitutions at the same position. Mapping of the noise events onto the original raw read positions revealed a high number of events, or noise spikes, occurring at specific positions within a given sequencing run. These noise spikes affected reads across the entire run, agnostic of locus or sample, while the position, occurrence, and amplitude of the spikes differed across runs. The majority of noise sequences with high read counts in a DNA profile were generated from base changes at these spike positions, and could be classified as noise spike artefacts. In this paper we present evidence of the noise spike artefacts and their genesis during the sequencing process in the sequencing-by-synthesis (SBS) cycles, as well as the methods developed to detect them. The information and methods will assist laboratories with detecting noise spikes in MiSeq FGx sequencing runs, differentiating authentic allele sequences from noise spike artefacts, and developing protocols for analyst review and handling of MiSeq FGx data.</div> </div> </div> </div> </div> <div class="f15 panel-box rounded shadow-none border"> <h4 class="mt-0 heading-count">Authors</h4> <div class="mb-3"> <article-authors tid="83094956" list="[{"name":"Yao-Yuan Liu","sequence":1},{"name":"Kevin Cheng","sequence":2},{"name":"Rebecca Just","sequence":3},{"name":"Sana Enke","sequence":4},{"name":"Jo-Anne Bright","sequence":5}]" verified="[]" page="work" ></article-authors> </div> <div class="alert alert-warning mb-0"> <h5 class="mt-0 bg-warning text-dark px-3 rounded d-inline-block"> I am an author on this paper </h5> <div class="font-weight-bold f13"> Click your name to claim this paper and add it to your profile. </div> </div> </div> <div class="f15 panel-box rounded shadow-none border"> <h4 class="mt-0 heading-count">Reviews</h4> <div class="d-flex flex-wrap flex-md-nowrap"> <div class="flex-grow-1"> <h4 class="f16"> Primary Rating <a href="javascript:;" data-toggle="tooltip" data-placement="right" title="The primary rating indicates the level of overall quality for the paper."> <i class="ivu-icon ivu-icon-md-help-circle f18 ml-2"></i> </a> </h4> <div class="d-flex flex-wrap flex-md-nowrap align-items-center alert mb-0"> <div class="d-flex align-items-center justify-content-center"> <Rate disabled allow-half value="4.5" style="font-size: 28px;"></Rate> <strong class="f20 m-3" style="color: #f5a623;">4.5</strong> </div> <div class="text-muted mx-4"> Not enough ratings </div> </div> <h4 class="f16"> Secondary Ratings <a href="javascript:;" data-toggle="tooltip" data-placement="right" title="Secondary ratings independently reflect strengths or weaknesses of the paper."> <i class="ivu-icon ivu-icon-md-help-circle f18 ml-2"></i> </a> </h4> <div class="d-flex flex-wrap flex-md-nowrap alert"> <div class="d-flex flex-shrink-0 align-items-center mr-3"> <h5 class="my-0">Novelty</h5> <strong class="mx-4">-</strong> </div> <div class="d-flex flex-shrink-0 align-items-center mr-3"> <h5 class="my-0">Significance</h5> <strong class="mx-4">-</strong> </div> <div class="d-flex flex-shrink-0 align-items-center mr-3"> <h5 class="my-0">Scientific rigor</h5> <strong class="mx-4">-</strong> </div> </div> </div> <div class="flex-shrink-0"> <div class="border bg-light py-2 px-4"> <h5 class="mb-1">Rate this paper</h5> <Rate class="f24" @on-change="function(value){ location.href='https://www.peeref.com/works/83094956/comments?rating='+value }"></Rate> </div> </div> </div> </div> <div id="collection" class="f15 panel-box rounded shadow-none border"> <h4 class="mt-0 heading-count">Recommended</h4> <div class="my-3"> <ul class="nav nav-pills border-bottom pb-3" style="list-style: none; padding-left: 0;"> <li class="active"> <a href="#articles_from_related" data-toggle="tab" class="mx-0 f15"> <strong>Related</strong> </a> </li> <li class=""> <a href="#articles_from_authors" data-toggle="tab" class="mx-0 f15"> <strong>From Same Authors</strong> </a> </li> <li class=""> <a href="#articles_from_journal" data-toggle="tab" class="mx-0 f15"> <strong>From Same Journal</strong> </a> </li> </ul> <div class="tab-content"> <div id="articles_from_related" class="tab-pane active"> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/22039499" class="text-dark hover-underline">Parallel sequencing of 87 STR and 294 SNP markers using the prototype of the SifaMPS panel on the MiSeq FGx™ system</a> </h4> <p class="text-ellipsis-2">Ruiyang Tao, Shouyu Wang, Anqi Chen, Ruocheng Xia, Xiaochun Zhang, Qi Yang, Yiling Qu, Suhua Zhang, Chengtao Li</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/2908.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> The study introduced a new panel designed for simultaneous targeting of 87 STRs and 294 SNPs using massively parallel sequencing technology, with most loci showing good performance in enhancing efficiency for human identification and paternity testing. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2021) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/22039499/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biochemical Research Methods </span> </div> <h4> <a href="https://www.peeref.com/works/82536892" class="text-dark hover-underline">Pilot validation of on-field STR typing and human identity testing by MinION nanopore sequencing</a> </h4> <p class="text-ellipsis-2">Yuan Luo, Jiarong Zhang, Ming Ni, Zhusong Mei, Qiao Ye, Bingqian Guo, Longmei Fang, Dongyun Feng, Lu Wang, Jiangwei Yan, Guangyun Wang</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/2465.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This study demonstrates for the first time that nanopore sequencing platforms can be used for on-site individual identification. Through rational design, the equipment is integrated into a portable detection box, enabling individual identification of 12 samples within approximately 24 hours. Double-blind experiments verify the accuracy of the method, with a genotyping success rate of 95.3% and an accuracy rate of 86.87% for 1150 genotypes. This study shows the feasibility of the whole process test, and it is expected that nanopore sequencing technology will be further improved for real-time individual identification at accident sites in the future. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ELECTROPHORESIS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/82536892/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/28382357" class="text-dark hover-underline">Molecular diversity of 23-YSTR markers in Iraqi populations</a> </h4> <p class="text-ellipsis-2">Mohammed Mahdi Al-Zubaidi, Majeed Arsheed Sabbah, Hanan Khaleel Mahmood</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/2998.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This study aimed to analyze the distribution of Y chromosome haplotypes in native Iraqi males and compare it with neighboring Arab populations. The results showed high genetic diversity in the Iraqi population, with the most common haplogroups being J1 and J2. This study provides valuable reference data for the Iraqi database. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">GENE</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/28382357/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/21885564" class="text-dark hover-underline">Massively parallel sequencing of 25 autosomal STRs including SE33 in four population groups for forensic applications</a> </h4> <p class="text-ellipsis-2">Ye-Lim Kwon, Bo Min Kim, Eun Young Lee, Kyoung-Jin Shin</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/8695.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This study utilized MPS technology to analyze 25 STRs from 350 samples of four populations, revealing that the sequence-based MPS method showed a higher number of alleles and unique alleles in SE33 compared to the length-based CE method. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENTIFIC REPORTS</span> (2021) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/21885564/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/26052839" class="text-dark hover-underline">Associations between forensic loci and expression levels of neighboring genes may compromise medical privacy</a> </h4> <p class="text-ellipsis-2">Mayra M. Banuelos, Yuomi Jhony A. Zavaleta, Alennie Roldan, Rochelle-Jan Reyes, Miguel Guardado, Berenice Chavez Rojas, Thet Nyein, Ana Rodriguez Vega, Maribel Santos, Emilia Huerta-Sanchez, Rori V. Rohlfs</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/6895.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This study reveals significant correlations between forensic STRs and neighboring gene expression levels, suggesting that forensic genetic loci may reveal expression levels and potential medical information. These findings have important implications for criminal investigations and genomic research. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/26052839/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/27322050" class="text-dark hover-underline">Developmental validation of the ForenSeq MainstAY kit, MiSeq FGx sequencing system and ForenSeq Universal Analysis Software</a> </h4> <p class="text-ellipsis-2">Kathryn M. Stephens, Richelle Barta, Keenan Fleming, Juan Carlos Perez, Shan-Fu Wu, June Snedecor, Cydne L. Holt, Bobby LaRue, Bruce Budowle</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/2908.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> For human identification purposes, forensic genetics traditionally relies on short tandem repeat (STR) markers amplified by polymerase chain reaction (PCR) and detected with capillary electrophoresis (CE). However, massively parallel sequencing (MPS) has advantages over CE-based typing, such as higher throughput capacity, increased discrimination power, and the ability to analyze a wide range of genetic markers. The developmental validation of the ForenSeq MainstAY library preparation kit with the MiSeq FGx Sequencing System and ForenSeq Universal Software demonstrates the sensitivity, accuracy, and suitability of MPS for casework. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/27322050/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Medicine, Legal </span> </div> <h4> <a href="https://www.peeref.com/works/23486326" class="text-dark hover-underline">19th century family saga re-told by DNA recovered from postcard stamps</a> </h4> <p class="text-ellipsis-2">Cordula Haas, Christian Koerner, Andrea Sulzer, Adelgunde Kratzer</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/2907.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> By analyzing ancient DNA from pre-war postage stamps, a family saga was unraveled, proving that an illegitimate child was actually the legitimate child of a married couple. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/23486326/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biotechnology & Applied Microbiology </span> </div> <h4> <a href="https://www.peeref.com/works/26430890" class="text-dark hover-underline">Population genetic study of 17 Y-STR Loci of the Sorani Kurds in the Province of Sulaymaniyah, Iraq</a> </h4> <p class="text-ellipsis-2">Balnd M. Albarzinji, Farhad M. Abdulkarim, Shaho A. Hussein, Dlshad Rashid, Hayder Lazim</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/1255.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This study investigates the population relationship of the Sorani Kurdish population in Sulaymaniyah province using DNA samples. The results show that the J2 subclade predominates in this population, and they cluster with Asian populations while being furthest from European and African populations. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">BMC GENOMICS</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/26430890/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83533487" class="text-dark hover-underline">Performance comparison of a previously validated microhaplotype panel and a forensic STR panel for DNA mixture analysis</a> </h4> <p class="text-ellipsis-2">J. Gonzalez-Bao, A. Mosquera-Miguel, L. Casanova-Adan, A. Ambroa-Conde, J. Ruiz-Ramirez, A. Cabrejas-Olalla, M. Boullon-Cassau, A. Freire-Aradas, A. Rodriguez-Lopez, C. Roth, R. Lagace, C. Phillips, M. V. Lareu, M. de la Puente</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/2908.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This study compared the performance of microhaplotypes (MHs) and standard STR kits in forensic genetics. The MH panel performed better in detecting 2-contributor mixtures, but worse with multiple contributors. MHs had higher insertion rates and lower dropout rates, were better at recovering the alleles of the minor contributor, and provided higher LR values. The results of estimating the contributor ratio using EuroForMix were also promising. Overall, the mixture detection performance of the MH panel was better than or equal to the standard STR panel. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83533487/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biochemistry & Molecular Biology </span> </div> <h4> <a href="https://www.peeref.com/works/28590355" class="text-dark hover-underline">Concordance study on Y-STRs typing between SeqStudio™ genetic analyzer for HID and MiSeq™ FGx forensic genomics system</a> </h4> <p class="text-ellipsis-2">Soldati Giulia, Turrina Stefania, Treccani Mirko, Saccardo Chiara, Ausania Francesco, De Leo Domenico</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/5943.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This study compares the typing results between CE and MPS techniques, and demonstrates the ability of MPS to produce reliable profiles despite generating a large amount of raw data. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">MOLECULAR BIOLOGY REPORTS</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/28590355/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/28372711" class="text-dark hover-underline">High-resolution genotyping of 58 STRs in 635 Northern Han Chinese with MiSeq FGx (R) Forensic Genomics System</a> </h4> <p class="text-ellipsis-2">Fei Guo, Ze Liu, Guannan Long, Biao Zhang, Xinyu Dong, Dahua Liu, Shaobo Yu</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/2908.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> In this study, sequence polymorphisms at autosomal, X chromosomal, and Y chromosomal STRs were analyzed in 635 Northern Han Chinese using the ForenSeq DNA Signature Prep Kit on the MiSeq FGx Forensic Genomics System. The results showed an increase in unique alleles and gene diversity between sequence and length, as well as the identification of novel variants compared to previous studies. The ForenSeq DNA Signature Prep Kit demonstrated high concordance with commercial CE kits and the MiSeq FGx system exhibited high-quality performance. Overall, this study establishes a high-resolution and high-quality dataset for human identification and population genetic studies. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/28372711/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/28820037" class="text-dark hover-underline">Genetic Polymorphism Analysis of 24 Y-STRs in a Han Chinese Population in Luzhou, Southwest China</a> </h4> <p class="text-ellipsis-2">Jiewen Fu, Binghui Song, Jie Qian, Ting He, Hanchun Chen, Jingliang Cheng, Junjiang Fu</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/9770.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This study analyzed the Y-STRs genetic data of male individuals from the Han population in Luzhou, demonstrating high polymorphism. Comparisons with 11 other related Han populations revealed close genetic relationships between Luzhou Han people and Han populations in Sichuan, Guangdong, and Hunan, consistent with geographical distribution, historical influence, and economic development. These findings are of great value for forensic applications and population genetics research. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">GENES</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/28820037/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Medicine, Legal </span> </div> <h4> <a href="https://www.peeref.com/works/21013204" class="text-dark hover-underline">Forensic application and genetic diversity of 21 autosomal STR loci in five major population groups of Pakistan</a> </h4> <p class="text-ellipsis-2">Muhammad Adnan Shan, Claus Borsting, Niels Morling</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/3787.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This study investigated the genetic diversity of different ethnic groups in Pakistan, showing significant genetic distances between them. SE33 exhibited the highest discrimination power across all populations, while D12S391 and D2S441 displayed deviation from Hardy-Weinberg equilibrium in specific populations. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">INTERNATIONAL JOURNAL OF LEGAL MEDICINE</span> (2021) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/21013204/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Anthropology </span> </div> <h4> <a href="https://www.peeref.com/works/23288079" class="text-dark hover-underline">Genetic analysis of sixteen autosomal STR loci in three Tunisian populations from Makthar, Nabeul and Sousse</a> </h4> <p class="text-ellipsis-2">Rim Al-Haj-Taib, Abir Mejri, Claus Borsting, Vania Pereira, Sarra Elkamel, Rene J. Herrera, Amel Benammar-Elgaaied, Karima Fadhlaoui-Zid</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/611.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This study estimated the genetic diversity and structure of three Tunisian populations using autosomal STRs. The results showed that these populations have genetic affinity with geographically close populations. There were no genetic differences between the Tunisian populations, but significant variance was observed within the same group, indicating heterogeneity. The application of autosomal STRs in anthropological and forensic studies is strongly supported by the results. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANNALS OF HUMAN BIOLOGY</span> (2021) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/23288079/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 "> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biochemistry & Molecular Biology </span> </div> <h4> <a href="https://www.peeref.com/works/82066705" class="text-dark hover-underline">Analysis of 26 STR loci (PowerPlex® Fusion 6C System) in a mestizo population from Mexico city</a> </h4> <p class="text-ellipsis-2">Mauro Lopez-Armenta, Maria Elizbeth Alvarez-Sanchez, Carolina Leon-Campos, Blanca Zoila Gonzalez-Sobrino, Hector Rangel-Villalobos, Jose Francisco Munoz-Valle, Astrid Desiree Sanchez-Mendez, Jose Alonso Aguilar-Velazquez</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/5943.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This study genotyped 600 DNA samples from Mexico City using the PowerPlex Fusion 6C System, which includes 26 STRs. The results demonstrated the efficacy of this system for human identification in the studied population and its potential application in other Mexican Mestizo populations. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">MOLECULAR BIOLOGY REPORTS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/82066705/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> </div> <div id="articles_from_authors" class="tab-pane "> <div class="nodata my-4">No Data Available</div> </div> <div id="articles_from_journal" class="tab-pane "> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83039808" class="text-dark hover-underline">Application of a newly constructed NGS panel with 45 X-linked microhaplotypes demonstrates the unique value of X-MH for kinship testing and mixture analysis</a> </h4> <p class="text-ellipsis-2">Guanju Ma, Kailiang Liu, Chaolong Lu, Qingqing Du, Mengjie Zhang, Qian Wang, Guangping Fu, Junyan Wang, Chunling Ma, Bin Cong, Shujin Li, Lihong Fu</p> <div class="d-flex mb-3"> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This study screened 63 X-MHs, evaluated their performance, and calculated population parameters. The panel performed well in personal identification and paternity testing, and showed unique advantages in complex kinship and male DNA mixture analyses. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83039808/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83225861" class="text-dark hover-underline">Profiling age and body fluid DNA methylation markers using nanopore adaptive sampling</a> </h4> <p class="text-ellipsis-2">Zaka Wing-Sze Yuen, Somasundhari Shanmuganandam, Maurice Stanley, Simon Jiang, Nadine Hein, Runa Daniel, Dennis McNevin, Cameron Jack, Eduardo Eyras</p> <div class="d-flex mb-3"> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> DNA methylation plays a crucial role in physiological processes and can be used as a biomarker for body fluid identification and age prediction. Current methylation detection methods rely on various techniques and markers, requiring specialized DNA preparation and biochemical treatments. This study used nanopore adaptive sampling technology to simultaneously identify age-associated and body fluid-specific methylation markers without the need for specialized DNA preparation or biochemical treatments. The technology was consistent with whole-genome bisulfite sequencing data and identified new sites strongly correlated with age. This study lays the foundation for the development of nanopore-based methods for age prediction and body fluid identification. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83225861/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83112863" class="text-dark hover-underline">Improved individual identification in DNA mixtures of unrelated or related contributors through massively parallel sequencing</a> </h4> <p class="text-ellipsis-2">Zhiyong Liu, Enlin Wu, Ran Li, Jiajun Liu, Yu Zang, Bin Cong, Riga Wu, Bo Xie, Hongyu Sun</p> <div class="d-flex mb-3"> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This study evaluated the impact of potential kinship on individual identification, including MPS performance, the influence of genetic markers on kinship and NOC inference, the probability distribution of MAC and TAC, trends in LR values, and comparisons of length- and sequence-based STR genotypes. Results showed that multiple genetic markers improved the accuracy of kinship and NOC inference, the LR value of the POI depended on the mixing ratio, and the correct kinship hypothesis yielded more conservative LR values. In addition, using sequence-based STR genotypes increased the power of individual identification and the accuracy of mixture ratio inference. The MGIEasy Signature Identification Library Prep kit demonstrated robust individual identification capabilities and is suitable for forensic DNA mixture interpretation. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83112863/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83547292" class="text-dark hover-underline">Forensic efficiency evaluation of a mtDNA whole genome sequencing system constructed with long fragment amplification strategy on DNA nanoball sequencing platform</a> </h4> <p class="text-ellipsis-2">Man Chen, Chong Chen, Ning Li, Yuerong Su, Wei Cui, Yan Huang, Meiming Cai, Bofeng Zhu</p> <div class="d-flex mb-3"> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This study evaluated a novel mtDNA whole genome sequencing system using long fragment amplification strategy on the DNA nanoball sequencing platform. The system demonstrated high sequencing quality and specific mtDNA sequencing efficiencies on positive control DNA and FTA bloodstain samples. In addition, the system sequencing efficiency was also confirmed among different kinds of samples. In summary, the system showed high performance in analyzing mtDNA sequence information, and had great prospects in forensic application and maternal genetic research. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83547292/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83110084" class="text-dark hover-underline">Shedding more light on shedders</a> </h4> <p class="text-ellipsis-2">Piyamas Petcharoen, Madison Nolan, K. Paul Kirkbride, Adrian Linacre</p> <div class="d-flex mb-3"> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This study tested 100 individuals to demonstrate the reproducibility of the cell staining process in a large population. The results showed that 98 donors had consistent and reproducible cell number deposition, with no difference between the left and right thumbs, and more cells deposited by males than females. The study also suggested that shedder status may be a continuum phenomenon. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83110084/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83010213" class="text-dark hover-underline">Saliva-derived secondary DNA transfer on fabric: The impact of varying conditions</a> </h4> <p class="text-ellipsis-2">Melanie S. Gegar, German A. Cisneros, Joanne Cox, Melanie Richard, Krista A. Currie</p> <div class="d-flex mb-3"> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This study investigated the secondary DNA transfer of saliva on different fabrics, including the effects of saliva moisture, contact methods, and other factors on DNA transfer. The study found that nylon fabric is more likely to transfer DNA than cotton and towel fabric, and wet saliva is more likely to transfer DNA than dry and rehydrated saliva. Active pressure is more likely to transfer DNA than controlled pressure. In addition, the study found that in some cases, the amount of DNA transferred to the secondary fabric is sufficient for STR-PCR amplification. These findings help us better understand the mechanism of DNA transfer and provide useful information for forensic science and criminal investigations. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83010213/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83645405" class="text-dark hover-underline">Rapid detection of blood using a novel application of RT-RPA integrated with CRISPR-Cas: ALAS2 detection as a model</a> </h4> <p class="text-ellipsis-2">Chih-Wen Su, Yi-Che Hsu, Li-Chin Tsai, James Chun- Lee, Adrian Linacre, Hsing-Mei Hsieh</p> <div class="d-flex mb-3"> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> A blood test method based on recombinase polymerase amplification, CRISPR-Cas, and lateral flow assay is reported, which is rapid, sensitive, and specific, and can detect the ALAS2 marker in blood. The test can use extracted RNA or directly added body fluids as templates, with a low detection limit, and can only detect blood, not other body fluids. When peripheral blood is mixed with saliva or semen, the test results will be affected. This method is expected to be applied in places far from the laboratory, such as crime scenes. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83645405/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/82908069" class="text-dark hover-underline">Unique molecular identifier-based amplicon sequencing of microhaplotypes for background noise mitigation</a> </h4> <p class="text-ellipsis-2">Ye-Lim Kwon, Kyoung-Jin Shin</p> <div class="d-flex mb-3"> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This study designed a UMI-based amplicon sequencing system, MH-UMIseq, which can simultaneously amplify 46 MHs and generate MPS libraries in four steps. The performance of the system was evaluated using Illumina NextSeq 550 and MiniSeq systems, and the results showed that the system can significantly suppress background noise, and the proportion of unsuppressed noise decreased significantly with the increase of input DNA. Therefore, the resolution of the MH-UMIseq system is expected to be higher than that of traditional MPS. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/82908069/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/82936722" class="text-dark hover-underline">Pairwise kinship inference and pedigree reconstruction using 91 microhaplotypes</a> </h4> <p class="text-ellipsis-2">Yifan Wei, Qiang Zhu, Haoyu Wang, Yueyan Cao, Xi Li, Xiaokang Zhang, Yufang Wang, Ji Zhang</p> <div class="d-flex mb-3"> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This study solves the problem of kinship inference without prior assumptions by genotyping and calculating the likelihood ratio to infer the relatedness of individuals. It is found that increasing the number of polymorphic loci can improve the accuracy of kinship inference, and correct classification can be achieved by expanding the suspected relationship. In addition, this method can also be used to reconstruct the pedigree of multiple individuals with unknown relationships. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/82936722/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83140740" class="text-dark hover-underline">A novel type of three band pattern at STR loci</a> </h4> <p class="text-ellipsis-2">B. Rolf, A. Phillip, K. Hannig, S. Koehler, I. Goettesdorfer</p> <div class="d-flex mb-3"> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> SE33 or ACTBP2 is the most polymorphic locus in many national DNA databases and commercial STR kits. This article describes the molecular reason for the three-band pattern of SE33, which is caused by the SNP in the flanking SE33 region that leads to the binding of the unlabeled D3S1358 primer, generating a chimeric PCR product smaller than the regular SE33 amplicon. This three-band pattern is called Type 3, and its genetic basis is different from Type 1 and Type 2. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83140740/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83347599" class="text-dark hover-underline">'Low' LRs obtained from DNA mixtures: On calibration and discrimination performance of probabilistic genotyping software</a> </h4> <p class="text-ellipsis-2">M. McCarthy-Allen, O. Bleka, R. Ypma, P. Gill, C. Benschop</p> <div class="d-flex mb-3"> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This study evaluated the performance of various PG software in the low LR range using the PROVEDIt dataset and found that previously reported LR thresholds may be too high, suggesting that they be lowered or discarded. The study also emphasizes the importance of calibration metrics in understanding the performance of PG systems. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83347599/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83754813" class="text-dark hover-underline">Comprehensive body fluid identification and contributor assignment by combining targeted sequencing of mRNA and coding region SNPs</a> </h4> <p class="text-ellipsis-2">Maximilian Neis, Theresa Gross, Harald Schneider, Peter M. Schneider, Cornelius Courts</p> <div class="d-flex mb-3"> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> This article describes the development of an mRNA/cSNP panel for forensic genetic analysis, which can provide crucial contextualizing information on the source level about a trace's composition and reduce the risk of association fallacies by typing individual coding region SNPs. The panel has high reliable detection sensitivity and low RNA input, and further optimization and improvement are needed in the future. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83754813/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/83775519" class="text-dark hover-underline">A multiplex microbial profiling system for the identification of the source of body fluid and skin samples</a> </h4> <p class="text-ellipsis-2">Hewen Yao, Yanyun Wang, Shuangshuang Wang, Chaoran Sun, Yuxiang Zhou, Lanrui Jiang, Zefei Wang, Xindi Wang, Zhirui Zhang, Tingting Yang, Feng Song, Haibo Luo</p> <div class="d-flex mb-3"> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> In this study, a multiplex system with seven microbial markers was developed to distinguish between skin, saliva, and feces samples. The system accurately identified sample types by generating specific electropherograms and was further tested and classified by beta diversity analysis and skin microenvironment cluster analysis. In addition, a machine learning prediction model was established to identify the skin microenvironment with an accuracy of 79%. These findings provide new insights into the application of microbial markers in forensic science. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83775519/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/27322024" class="text-dark hover-underline">Kinship analysis of skeletal remains from the Middle Ages</a> </h4> <p class="text-ellipsis-2">Mirela Dzehverovic, Belma Jusic, Amela Pilav, Tamara Lukic, Jasmina Cakar</p> <div class="d-flex mb-3"> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> Medieval cemeteries in Travnik, Bosnia and Herzegovina were excavated from 2011 to 2014, revealing skeletal remains of 11 individuals. Genetic analysis was conducted on these remains to test familial relationships and predict Y-haplogroup. Autosomal and Y-STR markers were analyzed, and kinship analysis showed direct brother-brother relatives with a high probability. Y-STR profiles indicated the same paternal lineage and J2a haplogroup for all male individuals. This study highlights the importance of utilizing STR markers and additional markers like Y-STRs in archaeogenetic studies to obtain comprehensive information on relatives and ancestry. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/27322024/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 "> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Genetics & Heredity </span> </div> <h4> <a href="https://www.peeref.com/works/27322030" class="text-dark hover-underline">DNA transfer when using gloves in burglary simulations</a> </h4> <p class="text-ellipsis-2">L. Carrara, T. Hicks, L. Samie, F. Taroni, V. Castella</p> <div class="d-flex mb-3"> <div class="p-3 rounded" style="background-color: #e8f3ff;"> <strong>Summary:</strong> Several studies have shown that DNA can be transferred from an individual to a surface indirectly. This means that finding DNA that matches a person does not necessarily prove that they have touched the surface where the DNA was found. This research simulated cases where DNA was recovered from a door handle and compared it to reference DNA profiles, emphasizing the need to evaluate DNA results when the person of interest has a legitimate reason for their DNA to be present. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">FORENSIC SCIENCE INTERNATIONAL-GENETICS</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/27322030/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="modal fade" id="export-citation" tabindex="-1"> <div class="modal-dialog"> <div class="modal-content"> <div class="modal-header"> <button type="button" class="close" data-dismiss="modal"><span>×</span></button> <h4 class="modal-title">Export Citation <b class="text-primary"></b></h4> </div> <div class="modal-body"> 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