Search results for: UL83/pp65

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UL83/pp65</title> <meta name="description" content="Search results for: UL83/pp65"> <meta name="keywords" content="UL83/pp65"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="UL83/pp65"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 4</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: UL83/pp65</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Humoral and Cytokine Responses to Major Human Cytomegalovirus Antigens in Mouse Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sahar%20Essa">Sahar Essa</a>, <a href="https://publications.waset.org/abstracts/search?q=Hussain%20A.%20Safar"> Hussain A. Safar</a>, <a href="https://publications.waset.org/abstracts/search?q=Raj%20Raghupathy"> Raj Raghupathy</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Human cytomegalovirus (CMV) continues to be a source of severe complications in immunologically immature and immunocompromised hosts. Effective CMV vaccines that help diminish CMV disease in transplant patients and avoid congenital infection are of great importance. Though the exact roles of defense mechanisms are unidentified, viral-specific antibodies and cytokine responses are known to be involved in controlling CMV infections. CMV envelope glycoprotein B (UL55/gB), matrix proteins (UL83/pp65, UL99/pp28, UL32/pp150), and assembly protein UL80a/pp38 are known to be targets of antiviral immune responses. We immunized mice intraperitoneally with these five CMV-related proteins (commercial) for their ability to induce specific antibody responses (in-house immunoassay) and cytokine production (commercial assay) in a mouse model. We observed a significant CMV-antigen-specific antibody response to pp38 and pp65 (E/C ˃2.0, p˂0.001). Mice immunized with pp38 had significantly higher concentrations of GM-CSF, IFN-α, IL-2 IL-4, IL-5, and IL-17A (p˂0.05). Mice immunized with pp65 showed significantly higher concentrations of GM-CSF, IFN-γ, IL-2 IL-4, IL-10, IL-12, IL-17A, and TNF-α. Th1 to Th2 cytokines ratios revealed a Th1 cytokine bias in mice immunized with pp38, pp65, pp150, and gB. We suggest that stimulation with multiple CMV-related proteins, which include pp38, pp65, and gB antigens, will allow both humoral and cellular immune responses to be efficiently activated, thus serving as appropriate CMV antigens for future vaccines. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cytomegalovirus" title="cytomegalovirus">cytomegalovirus</a>, <a href="https://publications.waset.org/abstracts/search?q=UL99%2Fpp28" title=" UL99/pp28"> UL99/pp28</a>, <a href="https://publications.waset.org/abstracts/search?q=UL80a%2Fpp38" title=" UL80a/pp38"> UL80a/pp38</a>, <a href="https://publications.waset.org/abstracts/search?q=UL83%2Fpp65" title=" UL83/pp65"> UL83/pp65</a>, <a href="https://publications.waset.org/abstracts/search?q=UL32%2Fpp150" title=" UL32/pp150"> UL32/pp150</a>, <a href="https://publications.waset.org/abstracts/search?q=UL55%2FgB" title=" UL55/gB"> UL55/gB</a>, <a href="https://publications.waset.org/abstracts/search?q=CMV-antigen-specific%20antibody" title=" CMV-antigen-specific antibody"> CMV-antigen-specific antibody</a>, <a href="https://publications.waset.org/abstracts/search?q=CMV%20antigen-specific%20cytokine%20responses" title=" CMV antigen-specific cytokine responses"> CMV antigen-specific cytokine responses</a> </p> <a href="https://publications.waset.org/abstracts/159929/humoral-and-cytokine-responses-to-major-human-cytomegalovirus-antigens-in-mouse-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/159929.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">82</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Humoral and Cellular Immune Responses to Major Human Cytomegalovirus Antigens in Mice Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20Essa">S. Essa</a>, <a href="https://publications.waset.org/abstracts/search?q=H.%20Safar"> H. Safar</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20Raghupathy"> R. Raghupathy</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Human cytomegalovirus (CMV) continues to be a source of severe complications to immunologically immature and immune-compromised hosts. Effective CMV vaccine that diminishes CMV disease in transplant patients and avoids congenital infection remains of high importance as no approved vaccines exist. Though the exact links of defense mechanisms are unidentified, viral-specific antibodies and Th1/Th2 cytokine responses have been involved in controlling viral infections. CMV envelope glycoprotein B (UL55/gB), the matrix proteins (UL83/pp65, UL99/pp28, UL32/pp150), and the assembly protein UL80a/pp38 are known to be targets of antiviral immune responses. In this study, mice were immunized with five HCMV antigens (UL32/pp150, UL80a/pp38, UL99/pp28, and UL83/pp65), and serum samples were collected and evaluated for eliciting viral-specific antibody responses. Moreover, Splenocytes were collected, stimulated, and assessed for cytokine responses. The results demonstrated a CMV-antigen-specific antibody response to pp38 and pp65 (E/C >2.0). The highest titers were detected with pp38 (average E/C 16.275) followed by pp65 (average E/C 7.72). Compared to control cells, splenocytes from PP38 antigen immunized mice gave a significantly higher concentration of GM-CSF, IFN-γ, IL-2 IL-4, IL-5, and IL-17A (P<0.05). Also, splenocytes from pp65 antigen immunized mice resulted in a significantly higher concentration of GM-CSF, IFN-γ, IL-2 IL-4, IL-10, IL-12, IL-17A, and TNF- α. The designation of target CMV peptides by identifying viral-specific antibodies and cytokine responses is vital for understanding the protective immune mechanisms during CMV infection and identifying appropriate viral antigens to develop novel vaccines. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=hepatitis%20C%20virus" title="hepatitis C virus">hepatitis C virus</a>, <a href="https://publications.waset.org/abstracts/search?q=peripheral%20blood%20mononuclear%20cells" title=" peripheral blood mononuclear cells"> peripheral blood mononuclear cells</a>, <a href="https://publications.waset.org/abstracts/search?q=neutrophils" title=" neutrophils"> neutrophils</a>, <a href="https://publications.waset.org/abstracts/search?q=cytokines" title=" cytokines"> cytokines</a> </p> <a href="https://publications.waset.org/abstracts/144387/humoral-and-cellular-immune-responses-to-major-human-cytomegalovirus-antigens-in-mice-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/144387.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">139</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Cut-Off of CMV Cobas® Taqman® (CAP/CTM Roche®) for Introduction of Ganciclovir Pre-Emptive Therapy in Allogeneic Hematopoietic Stem Cell Transplant Recipients</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=B.%20B.%20S.%20Pereira">B. B. S. Pereira</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20O.%20Souza"> M. O. Souza</a>, <a href="https://publications.waset.org/abstracts/search?q=L.%20P.%20Zanetti"> L. P. Zanetti</a>, <a href="https://publications.waset.org/abstracts/search?q=L.%20C.%20S.%20Oliveira"> L. C. S. Oliveira</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20R.%20P.%20Moreno"> J. R. P. Moreno</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20P.%20Souza"> M. P. Souza</a>, <a href="https://publications.waset.org/abstracts/search?q=V.%20R.%20Colturato"> V. R. Colturato</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20M.%20Machado"> C. M. Machado</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: The introduction of prophylactic or preemptive therapies has effectively decreased the CMV mortality rates after hematopoietic stem cell transplantation (HSCT). CMV antigenemia (pp65) or quantitative PCR are methods currently approved for CMV surveillance in pre-emptive strategies. Commercial assays are preferred as cut-off levels defined by in-house assays may vary among different protocols and in general show low reproducibility. Moreover, comparison of published data among different centers is only possible if international standards of quantification are included in the assays. Recently, the World Health Organization (WHO) established the first international standard for CMV detection. The real time PCR COBAS Ampliprep/ CobasTaqMan (CAP/CTM) (Roche®) was developed using the WHO standard for CMV quantification. However, the cut-off for the introduction of antiviral has not been determined yet. Methods: We conducted a retrospective study to determine: 1) the sensitivity and specificity of the new CMV CAP/CTM test in comparison with pp65 antigenemia to detect episodes of CMV infection/reactivation, and 2) the cut-off of viral load for introduction of ganciclovir (GCV). Pp65 antigenemia was performed and the corresponding plasma samples were stored at -20°C for further CMV detection by CAP/CTM. Comparison of tests was performed by kappa index. The appearance of positive antigenemia was considered the state variable to determine the cut-off of CMV viral load by ROC curve. Statistical analysis was performed using SPSS software version 19 (SPSS, Chicago, IL, USA.). Results: Thirty-eight patients were included and followed from August 2014 through May 2015. The antigenemia test detected 53 episodes of CMV infection in 34 patients (89.5%), while CAP/CTM detected 37 episodes in 33 patients (86.8%). AG and PCR results were compared in 431 samples and Kappa index was 30.9%. The median time for first AG detection was 42 (28-140) days, while CAP/CTM detected at a median of 7 days earlier (34 days, ranging from 7 to 110 days). The optimum cut-off value of CMV DNA was 34.25 IU/mL to detect positive antigenemia with 88.2% of sensibility, 100% of specificity and AUC of 0.91. This cut-off value is below the limit of detection and quantification of the equipment which is 56 IU/mL. According to CMV recurrence definition, 16 episodes of CMV recurrence were detected by antigenemia (47.1%) and 4 (12.1%) by CAP/CTM. The duration of viremia as detected by antigenemia was shorter (60.5% of the episodes lasted ≤ 7 days) in comparison to CAP/CTM (57.9% of the episodes lasting 15 days or more). This data suggests that the use of antigenemia to define the duration of GCV therapy might prompt early interruption of antiviral, which may favor CMV reactivation. The CAP/CTM PCR could possibly provide a safer information concerning the duration of GCV therapy. As prolonged treatment may increase the risk of toxicity, this hypothesis should be confirmed in prospective trials. Conclusions: Even though CAP/CTM by ROCHE showed great qualitative correlation with the antigenemia technique, the fully automated CAP/CTM did not demonstrate increased sensitivity. The cut-off value below the limit of detection and quantification may result in delayed introduction of pre-emptive therapy. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antigenemia" title="antigenemia">antigenemia</a>, <a href="https://publications.waset.org/abstracts/search?q=CMV%20COBAS%2FTAQMAN" title=" CMV COBAS/TAQMAN"> CMV COBAS/TAQMAN</a>, <a href="https://publications.waset.org/abstracts/search?q=cytomegalovirus" title=" cytomegalovirus"> cytomegalovirus</a>, <a href="https://publications.waset.org/abstracts/search?q=antiviral%20cut-off" title=" antiviral cut-off"> antiviral cut-off</a> </p> <a href="https://publications.waset.org/abstracts/56757/cut-off-of-cmv-cobas-taqman-capctm-roche-for-introduction-of-ganciclovir-pre-emptive-therapy-in-allogeneic-hematopoietic-stem-cell-transplant-recipients" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/56757.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">191</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> New Active Dioxin Response Element Sites in Regulatory Region of Human and Viral Genes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ilya%20B.%20Tsyrlov">Ilya B. Tsyrlov</a>, <a href="https://publications.waset.org/abstracts/search?q=Dmitry%20Y.%20Oshchepkov"> Dmitry Y. Oshchepkov</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A computational search for dioxin response elements (DREs) in genes of proteins comprising the Ah receptor (AhR) cytosolic core complex was performed by highly efficient tool SITECON. Eventually, the following number of new DREs in 5’flanking region was detected by SITECON: one in AHR gene, five in XAP2, eight in HSP90AA1, and three in HSP90AB1 genes. Numerous DREs found in genes of AhR and AhR cytosolic complex members would shed a light on potential mechanisms of expression, the stoichiometry of unliganded AhR core complex, and its degradation vs biosynthesis dynamics resulted from treatment of target cells with the AhR most potent ligand, 2,3,7,8-TCDD. With human viruses, reduced susceptibility to TCDD of geneencoding HIV-1 P247 was justified by the only potential DRE determined in gag gene encoding HIV-1 P24 protein, whereas the regulatory region of CMV genes encoding IE gp/UL37 has five potent DRE, 1.65 kb/UL36 – six DRE, pp65 and pp71 – each has seven DRE, and pp150 – ten DRE. Also, from six to eight DRE were determined with SITECON in the regulatory region of HSV-1 IE genes encoding tegument proteins, UL36 and UL37, and of UL19 gene encoding bindingglycoprotein C (gC). So, TCDD in the low picomolar range may activate in human cells AhR: Arnt transcription pathway that triggers CMV and HSV-1 reactivation by binding to numerous promoter DRE within immediate-early (IE) genes UL37 and UL36, thus committing virus to the lytic cycle. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=dioxin%20response%20elements" title="dioxin response elements">dioxin response elements</a>, <a href="https://publications.waset.org/abstracts/search?q=Ah%20receptor" title=" Ah receptor"> Ah receptor</a>, <a href="https://publications.waset.org/abstracts/search?q=AhR%3A%20Arnt%20transcription%20pathway" title=" AhR: Arnt transcription pathway"> AhR: Arnt transcription pathway</a>, <a href="https://publications.waset.org/abstracts/search?q=human%20and%20viral%20genes" title=" human and viral genes"> human and viral genes</a> </p> <a href="https://publications.waset.org/abstracts/150381/new-active-dioxin-response-element-sites-in-regulatory-region-of-human-and-viral-genes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/150381.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">104</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> 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Search results for: UL83/pp65