{"title":"Optimization of a Bioremediation Strategy for an Urban Stream of Matanza-Riachuelo Basin","authors":"Mar\u00eda D. Groppa, Andrea Trentini, Myriam Zawoznik, Roxana Bigi, Carlos Nadra, Patricia L. Marconi","volume":149,"journal":"International Journal of Environmental and Ecological Engineering","pagesStart":418,"pagesEnd":425,"ISSN":"1307-6892","URL":"https:\/\/publications.waset.org\/pdf\/10010411","abstract":"<p>In the present work, a remediation bioprocess based on the use of a local isolate of the microalgae <em>Chlorella vulgaris<\/em> immobilized in alginate beads is proposed. This process was shown to be effective for the reduction of several chemical and microbial contaminants present in Cild&aacute;&ntilde;ez stream, a water course that is part of the Matanza-Riachuelo Basin (Buenos Aires, Argentina). The bioprocess, involving the culture of the microalga in autotrophic conditions in a stirred-tank bioreactor supplied with a marine propeller for 6 days, allowed a significant reduction of <em>Escherichia coli<\/em> and total coliform numbers (over 95%), as well as of ammoniacal nitrogen (96%), nitrates (86%), nitrites (98%), and total phosphorus (53%) contents. Pb content was also significantly diminished after the bioprocess (95%). Standardized cytotoxicity tests using<em> Allium cepa<\/em> seeds and Cild&aacute;&ntilde;ez water pre- and post-remediation were also performed. Germination rate and mitotic index of onion seeds imbibed in Cild&aacute;&ntilde;ez water subjected to the bioprocess was similar to that observed in seeds imbibed in distilled water and significantly superior to that registered when untreated Cild&aacute;&ntilde;ez water was used for imbibition. Our results demonstrate the potential of this simple and cost-effective technology to remove urban-water contaminants, offering as an additional advantage the possibility of an easy biomass recovery, which may become a source of alternative energy.<\/p>\r\n","references":"[1]\tM. Nassir Khan, F. Mohamed, \u201cEutrophication: challenges and solutions,\u201d in Eutrophication: Causes, Consequences and Control, vol. 2, A. A. Ansari, S. S. Gill, Eds. Dordrecht: Springer Science+Business Media, 2014, pp. 1\u201316.\r\n[2]\tAgencia de Protecci\u00f3n Ambiental (APRA), Ministerio de Ambiente y Espacio P\u00fablico, Informe Anual Ambiental 2016, retrieved from: http:\/\/cdn2.buenosaires.gob.ar\/espaciopublico\/apra\/informe_anual_ambiental_2016.pdf. Accessed on 15\/10\/2018.\r\n[3]\tL. E. Bashan, Y. Bashan, M. Moreno, V. K. Lebsky, and J. J. 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