Yeast GST-tagged ORFs

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</option> <option class="nav-secondary__country-option" data-country="1" data-countryid="ZM" value="{EF4654D0-C25E-4538-96B6-88B1FF9FCF6D}"> Zambia </option> <option class="nav-secondary__country-option" data-country="1" data-countryid="ZW" value="{DDEB2EF4-A2E5-4276-917C-A8A5FEEC1307}"> Zimbabwe </option> </select> </div> </div> </nav> </div> </div> </header> <main class="site-main"> <div class="site-main__content"> <nav class="breadcrumbs" aria-label="Breadcrumb"> <ol class="breadcrumbs__list list-reset flex"> <li class="breadcrumbs__item"> <i class="breadcrumbs__icon fas fa-dna"></i> <i class="breadcrumbs__angle fas fa-chevron-right"></i> </li> <li class="breadcrumbs__item"> <a href="/en/non-mammalian-research-tools"> Non-mammalian research tools </a> <i class="breadcrumbs__angle fas fa-chevron-right"></i> </li> <li class="breadcrumbs__item" aria-current="page"> Yeast GST-tagged ORFs </li> </ol> </nav> <div class="pif pif--agno" data-pif-prodid="0F46BD60-93E1-4563-A4DD-A6FCAA9C4821"> <div class="pif__wrapper"> <header class="pif__row pif__row--header js-pif-row-header"> <div class="pif__col pif__col--heading"> <h2 class="pif__heading">Yeast GST-tagged ORFs</h2> <p class="pif__subtitle"></p> <div class="pif__lead-in rich-text">The Yeast GST-tagged ORFs are a a collection of more than 5,000 yeast strains that each overexpresses a different yeast open reading frame (ORF) when induced with galactose.</div> </div> <div class="pif__col pif__col--brand"> <img src="/-/media/Images/Horizon/logos/dharmacon-reagents-logo.jpg" width="220" height="155" title="Brand Image" class="brand-image" alt="Dharmacon" /> </div> </header> <section class="pif__row pif__row--gene js-pif-row-gene"> </section> <section class="pif__row pif__row--body js-spinner-visibility-toggle"> <div class="pif__col pif__col--search"> <h5 class="pif__heading pif__heading--search">Yeast GST-tagged ORFs</h5> <div class="pif__wrapper pif__wrapper--search"> <div class="pif__step"> <h3 class="pif__heading pif__heading--step"><span class="pif__icon">1</span>Start Here</h3> </div> <form id="pifgnosticsearch" class="search-bar search-bar--pif" role="search" action="JavaScript:void(0);"> <div class="search-bar__wrap"> <label for="search" class="hidden">Search</label> <input id="searchField" class="search-bar__input gtm-agnoSearchTerm" type="search" name="searchTerm" placeholder="Enter gene symbol, accession, or ID" autocomplete="off" value=""> <button class="search-bar__btn" data-pif-el="searchbtn"> <svg aria-hidden="true" focusable="false" data-prefix="fal" data-icon="search" class="search-bar__btn-icon svg-inline--fa fa-magnifying-glass fa-w-16 gtm-agnoSearchSuggestions" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M508.5 481.6l-129-129c-2.3-2.3-5.3-3.5-8.5-3.5h-10.3C395 312 416 262.5 416 208 416 93.1 322.9 0 208 0S0 93.1 0 208s93.1 208 208 208c54.5 0 104-21 141.1-55.2V371c0 3.2 1.3 6.2 3.5 8.5l129 129c4.7 4.7 12.3 4.7 17 0l9.9-9.9c4.7-4.7 4.7-12.3 0-17zM208 384c-97.3 0-176-78.7-176-176S110.7 32 208 32s176 78.7 176 176-78.7 176-176 176z"></path></svg> </button> </div> </form> </div> <div class="pif__wrapper pif__wrapper--search hidden" data-pif-el="results"> <div class="pif__step"> <h3 class="pif__heading pif__heading--step"><span class="pif__icon">2</span>Choose</h3> </div> <ul class="pif__search"></ul> </div> </div> </section> <section class="pif__row pif__row--footer js-pif-row-footer"> <div class="tabs"> <ul class="tabs__nav"> <li class="tabs__item" style="order:0"> <button id="description" class="tabs__button" type="button">Description</button> </li> <li class="tabs__item" style="order:1"> <button id="references" class="tabs__button" type="button">References</button> </li> <li class="tabs__item" style="order:2"> <button id="resources" class="tabs__button" type="button">Resources</button> </li> </ul> <div class="tabs__content" style="order:0"> <div class="rich-text"> <p><p>The Andrews lab at the University of Toronto has developed a collection of more than 5,000 yeast strains that each overexpresses a different yeast open reading frame (ORF) when induced with galactose.</p><p>More than 80% of the S. cerevisiae genome is represented. ORFs are plasmid-encoded, tagged at the N-terminus with GST, and expressed under control of the GAL1/10 promoter (Zhu et al, 2001). The Yeast GST-tagged ORFs is in a BY4741 (MATa) background and has been used for genome-scale overexpression screening (Sopko et al, 2006).</p><h6>Highlights</h6><ul><li>More than 5,000 yeast strains overexpress an ORF through galactose induction</li> <li> Collection represents more than 80% of the <em>S. cerevisiae</em> genome</li></ul><h6>Note</h6><p>We provide certain clone resources developed by leading academic laboratories. Many of these resources address the needs of specialized research communities not served by other commercial entities. In order to provide these as a public resource, we depend on the contributing academic laboratories for quality control.</p><p>Therefore, these are distributed in the format provided by the contributing institution "as is" with no additional product validation or guarantee. We are not responsible for any errors or performance issues. Additional information can be found in the product manual as well as in associated published articles (if available). Alternatively, the source academic institution can be contacted directly for troubleshooting.</p><p>Yeast strains are shipped in CM broth with glucose–uracil (Teknova cat # C7141) with 15% glycerol (added after growth of strains). Individual strains are shipped in 2 mL tubes at room temperature; the entire collection is shipped in 96-well plates on dry ice. All strains should be stored at –80°C.</p></p> </div> </div> <div class="tabs__content hidden" style="order:1"> <div class="rich-text"> <p><ol><li>R. Sopko <em>et al.</em>, <a href="http://www.ncbi.nlm.nih.gov/pubmed/16455487">Mapping pathways and phenotypes by systematic gene overexpression.</a> <em>Molecular Cell.</em> <strong>21(3)</strong>, 319–330 (3 February 2006).</li> <li>H. Zhu <em>et al.</em>, <a href="http://www.ncbi.nlm.nih.gov/pubmed?term=Global%20analysis%20of%20protein%20activities%20using%20proteome%20chips.">Global analysis of protein activities using proteome chips.</a> <em>Science.</em> <strong>293(5537)</strong>, 2101–2105 (14 September 2001).</li></ol></p> </div> </div> <div class="tabs__content hidden" style="order:2"> <section class="resources"> <div class="resources__category"> <h3 class="resource__title">Product data</h3> <ul class="resources__list"> <li class="resources__item"> <div class="resources__icon"> <i class="icon fal fa-file-excel"></i> </div> <a class="resource__link" target="_blank" href="/-/media/Files/Horizon/resources/Product-data/ysc4423-r39-r47.xls?sc_lang=en"> <span class="resource__description">YSC4423 YEAST GST Datafile</span> </a> </li> <li class="resources__item"> <div class="resources__icon"> <i class="icon fal fa-file-excel"></i> </div> <a class="resource__link" target="_blank" href="/-/media/Files/Horizon/resources/Product-data/ysc4613-yeast-genomic-tiling-path-v2-prelich.xls?sc_lang=en"> <span class="resource__description">YSC4613 Yeast Genomic Tiling Path v2 - Plate Map</span> </a> </li> </ul> </div> <div class="resources__category"> <h3 class="resource__title">Safety data sheets</h3> <ul class="resources__list"> <li class="resources__item"> <div class="resources__icon"> <i class="icon fal fa-file-pdf"></i> </div> <a class="resource__link" target="_blank" href="/-/media/Files/Horizon/resources/Safety-data-sheets/glycerol-stocks-sds-eu.pdf?sc_lang=en"> <span class="resource__description">SDS (EU) Glycerol Clones</span> </a> </li> <li class="resources__item"> <div class="resources__icon"> <i class="icon fal fa-file-pdf"></i> </div> <a class="resource__link" target="_blank" href="/-/media/Files/Horizon/resources/Safety-data-sheets/glycerol-stocks-sds-na.pdf?sc_lang=en"> <span class="resource__description">SDS (US) Glycerol Clones</span> </a> </li> </ul> </div> <div class="resources__category"> <h3 class="resource__title">Technical manuals</h3> <ul class="resources__list"> <li class="resources__item"> <div class="resources__icon"> <i class="icon fal fa-file-pdf"></i> </div> <a class="resource__link" target="_blank" href="/-/media/Files/Horizon/resources/Technical-manuals/yeast-gst-collect-strains-manual.pdf?sc_lang=en"> <span class="resource__description">Yeast GST Fusion Collection and Strains, YSC4423, YSC4515 - 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Yeast GST-tagged ORFs