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KEGG ENZYME: 1.14.13.234
<!doctype html> <html><head> <title>KEGG ENZYME: 1.14.13.234</title> <link type="text/css" rel="stylesheet" href="/css/gn2.css?1743636390"> <link type="text/css" rel="stylesheet" href="/css/bget.css?1743636390"> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <link type="text/css" rel="stylesheet" href="/css/bgetm.css?1743636390" media="only screen and (max-width: 768px)"> <style>.kcfd{display:none;}</style> <script> <!-- var getElementsByClassName = document.getElementsByClassName ? function (cl){ return document.getElementsByClassName(cl); } : function (cl){ var elms = []; var objs = document.getElementsByTagName('*'); for(var i=0;i<objs.length;i++){ var obj = objs[i]; if(obj.className == cl){ elms.push(obj); } } return elms; }; function toggleField(clk,cl,show,hide){ var objs = getElementsByClassName(cl); for(var i=0;i<objs.length;i++){ var obj = objs[i]; if(!obj.style.display||obj.style.display == 'none'){ obj.style.display = 'block'; clk.innerHTML = ' « '+hide; 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} } } function Link_XtrctSeq2(form) { var dna_from; var dna_to; var dna_len; var plus_up = Number(form.XtrctSeq_UP.value) ; var plus_down = Number(form.XtrctSeq_DOWN.value); var vector = Number(form.VECTOR.value); var org = form.ORG.value; var chr = form.CHR.value; var kid = form.KEGGID.value; var url; if (plus_up == 0 && plus_down == 0) { url = "/entry/-f+-n+n+" + kid; } else { if (vector == 1) { dna_from = Number(form.FROM.value) - plus_up; dna_to = Number(form.TO.value) + plus_down; } else { dna_from = Number(form.FROM.value) - plus_down; dna_to = Number(form.TO.value) + plus_up; } url = "/dbget-bin/cut_sequence_genes.pl?FROM=" + dna_from + "&TO=" + dna_to +"&VECTOR=" + vector + "&ORG=" + org; if (chr) url += "&CHR=" + chr; } //window.open( url, "_self" ); location.href = url; } function go_taxonomy(form,params){ for(var key in params){ if(form[key]){ form.setAttribute(key,params[key]); } else{ var obj = document.createElement('input'); obj.setAttribute('type','hidden'); obj.setAttribute('name',key); obj.setAttribute('value',params[key]); form.appendChild(obj); } } form.submit(); } window.onload=function(){window.focus();init();}; //---> </script> </head> <body> <div> <table border=0 cellpadding=0 cellspacing=0><tr><td> <table border=0 cellpadding=0 cellspacing=0 width="683"><tr><td width=70><a href="/kegg/kegg2.html"><img align="middle" alt="KEGG" border=0 src="/Fig/bget/kegg2.gif"></a></td><td> </td><td><a name="enzyme:1.14.13.234"></a><font class="title2">ENZYME: 1.14.13.234</font></td><td class="tar vbot"><button class="btn" onclick="javascript:void(window.open('/kegg/document/help_bget_enzyme.html','KEGG_Help','toolbar=no,location=no,directories=no,width=720,height=640,resizable=yes,scrollbars=yes'));return false;">Help</button></td></tr></table><form method="post" action="/kegg-bin/search_htext" enctype="application/x-www-form-urlencoded" id="form1" name="form1"></form><table class="w1" width="650"> <tr> <td class="fr2 w1"> <table width="650" class="w2"> <tr><th class="th20 deft tal vmid"><span class="nowrap">Entry</span></th> <td class="td20 defd"><table class="w1" width="100%"><tr><td class="tal pd0"><code><span class="nowrap">EC 1.14.13.234 Enzyme <br> </span></code></td></tr></table></td></tr> <tr><th class="th21 deft tal vtop"><span class="nowrap">Name</span></th> <td class="td21 defd"><div class="cel"><div class="cel">5a,11a-dehydrotetracycline 5-monooxygenase;<br> oxyS (gene name);<br> 12-dehydrotetracycline 5-monooxygenase<br> </div></div></td></tr> <tr><th class="th20 deft tal vtop"><span class="nowrap">Class</span></th> <td class="td20 defd"><div class="cel">Oxidoreductases;<br> Acting on paired donors, with incorporation or reduction of molecular oxygen;<br> With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor<br> <button class="btn" onclick="location.href='/brite/ko01000+1.14.13.234';return false;">BRITE hierarchy</button></div></td></tr> <tr><th class="th21 deft tal vtop"><span class="nowrap">Sysname</span></th> <td class="td21 defd"><div class="cel">5a,11a-dehydrotetracycline,NADPH:oxygen oxidoreductase (5-hydroxylating)<br> </div></td></tr> <tr><th class="th20 deft tal vtop"><span class="nowrap">Reaction(IUBMB)</span></th> <td class="td20 defd"><div class="cel">5a,11a-dehydrotetracycline + NADPH + H+ + O2 = 5a,11a-dehydrooxytetracycline + NADP+ + H2O [RN:<a href="/entry/R09198">R09198</a>]<br> </div></td></tr> <tr><th class="th21 deft tal vtop"><span class="nowrap">Reaction(KEGG)</span></th> <td class="td21 defd"><div class="cel"><a href="/entry/R09198">R09198</a><br> <button class="btn" onclick="location.href='/entry/R09198';return false;">Reaction</button></div></td></tr> <tr><th class="th20 deft tal vtop"><span class="nowrap">Substrate</span></th> <td class="td20 defd"><div class="cel">5a,11a-dehydrotetracycline [CPD:<a href="/entry/C03206">C03206</a>];<br> NADPH [CPD:<a href="/entry/C00005">C00005</a>];<br> H+ [CPD:<a href="/entry/C00080">C00080</a>];<br> O2 [CPD:<a href="/entry/C00007">C00007</a>]<br> </div></td></tr> <tr><th class="th21 deft tal vtop"><span class="nowrap">Product</span></th> <td class="td21 defd"><div class="cel"><div class="cel">5a,11a-dehydrooxytetracycline [CPD:<a href="/entry/C18296">C18296</a>];<br> NADP+ [CPD:<a href="/entry/C00006">C00006</a>];<br> H2O [CPD:<a href="/entry/C00001">C00001</a>]<br> </div></div></td></tr> <tr><th class="th20 deft tal vtop"><span class="nowrap">Comment</span></th> <td class="td20 defd"><div class="cel">The enzyme, characterized from the bacterium Streptomyces rimosus, is bifunctional, catalysing two successive monooxygenation reactions. It starts by catalysing the stereospecific hydroxylation of anhydrotetracycline at C-6 (EC <a href="/entry/1.14.13.38">1.14.13.38</a>). If the released product is captured by EC <a href="/entry/1.3.98.4">1.3.98.4</a>, 5a,11a-dehydrotetracycline dehydrogenase (OxyR), it is reduced to tetracycline. However, if the released product is recaptured by OxyS, it performs an additional hydroxylation at C-5, producing 5a,11a-dehydrooxytetracycline, which, following the action of OxyR, becomes oxytetracycline.<br> </div></td></tr> <tr><th class="th21 deft tal vtop"><span class="nowrap">History</span></th> <td class="td21 defd"><div class="cel">EC 1.14.13.234 created 2016<br> </div></td></tr> <tr><th class="th20 deft tal vtop"><span class="nowrap">Pathway</span></th> <td class="td20 defd"><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><a href="/pathway/ec00253+1.14.13.234">ec00253</a> </span></td><td>Tetracycline biosynthesis</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><a href="/pathway/ec01057+1.14.13.234">ec01057</a> </span></td><td>Biosynthesis of type II polyketide products</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><a href="/pathway/ec01100+1.14.13.234">ec01100</a> </span></td><td>Metabolic pathways</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><a href="/pathway/ec01110+1.14.13.234">ec01110</a> </span></td><td>Biosynthesis of secondary metabolites</td></tr></table></td></tr> <tr><th class="th21 deft tal vtop"><span class="nowrap">Orthology</span></th> <td class="td21 defd"><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><a href="/entry/K14256">K14256</a> </span></td><td>anhydrotetracycline 6-monooxygenase / 5a,11a-dehydrotetracycline 5-monooxygenase</td></tr></table></td></tr> <tr><th class="th20 deft tal vtop"><span class="nowrap">Genes</span></th> <td class="td20 defd"><style>span.g1a{color:#003399}span.g1a:hover{color:#33cc99;cursor:pointer;}div.g1{display:none;}</style><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><span class="g1a" id="g11">SVR</span>: </span></td><td id="g11d">CP971_23960</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><span class="g1a" id="g12">SRIM</span>: </span></td><td id="g12d">CP984_02460</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><span class="g1a" id="g13">SPLA</span>: </span></td><td id="g13d">CP981_34445</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><span class="g1a" id="g14">SDRZ</span>: </span></td><td id="g14d">NEH16_31700</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><span class="g1a" id="g15">SHAU</span>: </span></td><td id="g15d">K9S39_12950</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><span class="g1a" id="g16">SARM</span>: </span></td><td id="g16d">DVA86_06945</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><span class="g1a" id="g17">SCAJ</span>: </span></td><td id="g17d">O1G22_08880</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><span class="g1a" id="g18">SCHE</span>: </span></td><td id="g18d">QRN89_14740</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><span class="g1a" id="g19">STRV</span>: </span></td><td id="g19d">EZV63_00255</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><span class="g1a" id="g110">KAU</span>: </span></td><td id="g110d">B6264_18535</td></tr></table><div class="g1"><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><span class="g1a" id="g111">NEX</span>: </span></td><td id="g111d">NE857_24350</td></tr></table></div><div class="g1"><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><span class="g1a" id="g112">AGRA</span>: </span></td><td id="g112d">AGRA3207_005105 AGRA3207_005106</td></tr></table></div><div class="g1"><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><span class="g1a" id="g113">AORI</span>: </span></td><td id="g113d">SD37_34210</td></tr></table></div><div class="g1"><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><span class="g1a" id="g114">KUT</span>: </span></td><td id="g114d">JJ691_15570</td></tr></table></div><div class="g1"><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><span class="g1a" id="g115">CROS</span>: </span></td><td id="g115d">N8J89_05295</td></tr></table></div><div class="g1"><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><span class="g1a" id="g116">DAUR</span>: </span></td><td id="g116d">Daura_37815 Daura_37840</td></tr></table></div><div class="g1"><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><span class="g1a" id="g117">CCAZ</span>: </span></td><td id="g117d">COUCH_15960</td></tr></table></div><a href="javascript:void(0)" onclick="javascript:toggleField(this,"g1","show all","hide")"> » show all</a><br> <script>for(var i=1;i<=17;i++){if(document.getElementById('g1'+i)){document.getElementById('g1'+i).onclick = function(e){geg1(this,e);};}}function geg1(bobj){var id = bobj.id;var ab = document.getElementById(id).innerText;var dt = document.getElementById(id+'d');var st = dt.innerText.replace(/\([^\)]+\)/g,'').replace(/ /g,'+');var gt = '/entry/'+ab+'+'+st;location.href = gt;}</script><input type="hidden" name="query" form="form1" value="SVR SRIM SPLA SDRZ SHAU SARM SCAJ SCHE STRV KAU NEX AGRA AORI KUT CROS DAUR CCAZ"><input type="hidden" name="htext" form="form1" value="br08601.keg"><input type="hidden" name="option" form="form1" value="-a"><button class="btn" onclick="javascript:go_taxonomy(document.form1,{"action":"/kegg-bin/search_htext"});return false;">Taxonomy</button></td></tr> <tr><th class="th21 deft tal vtop"><span class="nowrap">Reference</span></th> <td class="td21 defd"><div class="cel">1 [PMID:<a href="https://www.ncbi.nlm.nih.gov/pubmed/2914874">2914874</a>]</div></td></tr> <tr><th class="th20 rept tal vtop"><span class="nowrap"> Authors</span></th> <td class="td20 repd"><div class="cel">Binnie C, Warren M, Butler MJ</div></td></tr> <tr><th class="th21 rept tal vtop"><span class="nowrap"> Title</span></th> <td class="td21 repd"><div class="cel">Cloning and heterologous expression in Streptomyces lividans of Streptomyces rimosus genes involved in oxytetracycline biosynthesis.</div></td></tr> <tr><th class="th20 rept tal vtop"><span class="nowrap"> Journal</span></th> <td class="td20 repd"><div class="cel">J Bacteriol 171:887-95 (1989)<br>DOI:<a href="https://doi.org/10.1128/JB.171.2.887-895.1989">10.1128/JB.171.2.887-895.1989</a></div></td></tr> <tr><th class="th21 deft tal vtop"><span class="nowrap">Reference</span></th> <td class="td21 defd"><div class="cel">2 [PMID:<a href="https://www.ncbi.nlm.nih.gov/pubmed/4959040">4959040</a>]</div></td></tr> <tr><th class="th20 rept tal vtop"><span class="nowrap"> Authors</span></th> <td class="td20 repd"><div class="cel">Miller PA, Saturnelli A, Martin JH, Itscher LA, Bohonos N</div></td></tr> <tr><th class="th21 rept tal vtop"><span class="nowrap"> Title</span></th> <td class="td21 repd"><div class="cel">A new family of tetracycline precursors. N-demethylanhydrotetracyclines.</div></td></tr> <tr><th class="th20 rept tal vtop"><span class="nowrap"> Journal</span></th> <td class="td20 repd"><div class="cel">Biochem Biophys Res Commun 16:285-91 (1964)<br>DOI:<a href="https://doi.org/10.1016/0006-291X(64)90341-9">10.1016/0006-291X(64)90341-9</a></div></td></tr> <tr><th class="th21 deft tal vtop"><span class="nowrap">Reference</span></th> <td class="td21 defd"><div class="cel">3 [PMID:<a href="https://www.ncbi.nlm.nih.gov/pubmed/3138982">3138982</a>]</div></td></tr> <tr><th class="th20 rept tal vtop"><span class="nowrap"> Authors</span></th> <td class="td20 repd"><div class="cel">Vancurova I, Volc J, Flieger M, Neuzil J, Novotna J, Vlach J, Behal V</div></td></tr> <tr><th class="th21 rept tal vtop"><span class="nowrap"> Title</span></th> <td class="td21 repd"><div class="cel">Isolation of pure anhydrotetracycline oxygenase from Streptomyces aureofaciens.</div></td></tr> <tr><th class="th20 rept tal vtop"><span class="nowrap"> Journal</span></th> <td class="td20 repd"><div class="cel">Biochem J 253:263-7 (1988)<br>DOI:<a href="https://doi.org/10.1042/bj2530263">10.1042/bj2530263</a></div></td></tr> <tr><th class="th21 deft tal vtop"><span class="nowrap">Reference</span></th> <td class="td21 defd"><div class="cel">4 [PMID:<a href="https://www.ncbi.nlm.nih.gov/pubmed/23621493">23621493</a>]</div></td></tr> <tr><th class="th20 rept tal vtop"><span class="nowrap"> Authors</span></th> <td class="td20 repd"><div class="cel">Wang P, Bashiri G, Gao X, Sawaya MR, Tang Y</div></td></tr> <tr><th class="th21 rept tal vtop"><span class="nowrap"> Title</span></th> <td class="td21 repd"><div class="cel">Uncovering the enzymes that catalyze the final steps in oxytetracycline biosynthesis.</div></td></tr> <tr><th class="th20 rept tal vtop"><span class="nowrap"> Journal</span></th> <td class="td20 repd"><div class="cel">J Am Chem Soc 135:7138-41 (2013)<br>DOI:<a href="https://doi.org/10.1021/ja403516u">10.1021/ja403516u</a></div></td></tr> <tr><th class="th21 deft tal vtop"><span class="nowrap">Other DBs</span></th> <td class="td21 defd"><table class="w1"><tr><td class="vtop pd0"><span class="nowrap">ExplorEnz - The Enzyme Database: </span></td><td><a href="https://www.enzyme-database.org/query.php?ec=1.14.13.234">1.14.13.234</a></td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap">IUBMB Enzyme Nomenclature: </span></td><td><a href="https://iubmb.qmul.ac.uk/enzyme/EC1/14/13/234.html">1.14.13.234</a></td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap">ExPASy - ENZYME nomenclature database: </span></td><td><a href="https://enzyme.expasy.org/EC/1.14.13.234">1.14.13.234</a></td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap">BRENDA, the Enzyme Database: </span></td><td><a href="https://www.brenda-enzymes.info/enzyme.php?ecno=1.14.13.234">1.14.13.234</a></td></tr></table></td></tr> <tr><th class="th20 deft tal vmid"><span class="nowrap">LinkDB</span></th> <td class="td20 defd"><button class="btn" onclick="location.href='https://www.genome.jp/dbget-bin/get_linkdb?enzyme+1.14.13.234';return false;">All DBs</button></td></tr> </table></td></tr></table> <br></td></tr></table> <a href="/dbget/">DBGET</a> integrated database retrieval system</div> </body> </html>