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'' : value; }, getCookie: function (key) { var result = ""; var cookies = document.cookie ? document.cookie.split('; ') : []; for (var i = 0; i < cookies.length; i++) { var parts = cookies[i].split('='); var name = decodeURIComponent(parts.shift()); var cookie = parts ? parts.join('=') : []; // Prevent storing or parsing of a cookie that we couldn't decode. if (cookie && key === name) { // removes surrounding quotes if (cookie.indexOf('"') === 0) { // This is a quoted cookie as according to RFC2068, unescape... cookie = cookie.slice(1, -1).replace(/\\"/g, '"').replace(/\\\\/g, '\\'); } // parse utf8 characters try { cookie = decodeURIComponent(cookie); } catch (e) {} result = cookie; // we found it, don't bother looking at other cookies break; } } return result; }, setCookie : function(name, value, numDays){ if ( !numDays ) numDays = 31; var expires = new Date(); expires.setTime(expires.getTime() + (1000 * 60 * 60 * 24 * numDays)); document.cookie = name+ '=' + escape(value) + '; path=/; expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); }, // generic function to set page attributes setPageAttribute: function (key, value) { this.page[key] = value; }, // display digitalData object displayDigitalData: function () { }, // specific function to add an object to digitalData addDigitDataObject: function (key, object) { this[key] = object; }, addChildObjectAttribute: function(child, key, value){ this[child][key] = value; }, initUserData: function(){ var gigyaCookie = this.getCookie("identity_uid"); this.user.gigyaId = ""; this.user.loginStatus = "anonymous"; if( gigyaCookie != ""){ this.user.gigyaId = gigyaCookie; this.user.loginStatus = "logged in"; } }, /** * Check if init has been initiated once * @type {Boolean} */ merchandisingDataInitted: false, initMerchandisingData: function () { // read cid and icid value from session cookie this.merchandising.icid = this.getCookie('s_ev21_tc'); this.merchandising.cid = this.getCookie('s_cmp_tc'); // EMID value this.merchandising.emid = this.getQueryParam("emid"); // referrer by domain if (typeof document.referrer !== 'undefined' && document.referrer.length > 0) { var masterDomain = this.getMasterDomain(); 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document.dispatchEvent(customClickEvent); } } } })(); window.digitalData.init(); </script>  <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_25"); </script> <script type="text/javascript"> var localizedStrings = { "ACCEPTANCE": "Acceptance of Terms of Use", "BTN_AGREE": "Agree", "BTN_DISAGREE": "Disagree", "PROCESSING_MESSAGE" :"processing ... please wait ...", "ADD_TO_CART_QUANTITY_ERROR":"You must provide a quantity to add an item to the cart", "ADD_TO_CART_VALID_QUANTITY_ERROR":"You must provide a valid quantity to add an item to the cart", "VIEW_CART" :"View cart & checkout", "VIEW_CONTACT_DETAIL" :"View Contact Details", "ADD_TO_CART_NOTIFICATION":"Add to Cart Notification", "UPDATE_CART" :"Update Cart", "PRINCIPAL_INVESTIGATOR":"Principal Investigator", "PRINCIPAL_INVESTIGATOR_TEAM_LEADER":"Principal Investigator or Team Leader", "PRINCIPAL_INVESTIGATOR_CHIEF":"Principal Investigator or Chief", "EMAIL_ERROR_1" :"Please Provide Collegues Email", "EMAIL_ERROR_2" :"Please enter a valid colleague email address", "EMAIL_ERROR_3" :"Please Provide Your Email", "EMAIL_ERROR_4" :"Please enter a valid email address", "ERROR_OCCURED_MESSAGE" :"The following errors occured", "NEXT" :"Next", "PREVIOUS" :"Prev", "IMAGE" :"Image", "SAVE_TO_LIST_ERROR_1" :"Please enter a list name.", "SAVE_TO_LIST_ERROR_2" :"Please enter a different list name that does not include the following: ?,/,\\,%,^,!", "MINICART_ERROR_1" :"Unable to connect to eCommerce system. 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height: 250px;"><h1 style="color: rgb(255, 255, 255);">Dynabeads Streptavidin Magnetic Bead Applications</h1><br><p><a href="#order"><span class="btn btn-featured">Order now</span></a> <a href="#resources"><span class="btn btn-primary">Resources for streptavidin beads</span></a></p></td></tr></tbody></table></div></div></div></div></div></div></div></div></div></div></div><div class="container page-header-hidden"><div class="row"><div class="span12"><div class="pageheading"></div></div></div></div><div class="container-wrap template-2-column"><div class="container"><div class="row"><script> var showHideText = { 'seeRelatedText':'See Navigation', 'hideRelatedText':'Hide Navigation' }; </script><div class="span3 sidebar" role="complementary"><div id="link-leftnav-toggle-container"><a class='link-leftnav-toggle' href='#'>See Navigation</a></div><div class="container-leftnav"><div class="railInheritParsys iparsys parsys"><div class="section"><div class="new"></div></div><div class="iparys_inherited"><div class="railInheritParsys iparsys parsys"><div class="autopopulatingleftnav section"><div class="leftnav-autopopulating"><ul class="nav nav-list"><li class="item-header nav-header"></li><li class="item-content collapse"><ul><li><a href="/sg/en/home/brands/product-brand/dynal/magnets.html">Magnetic Separation Stands and Racks</a></li><li><a href="/sg/en/home/brands/product-brand/dynal/hulamixer-sample-mixer.html">HulaMixer Rotating Tube Mixer</a></li><li><a href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads.html">Dynabeads Streptavidin Magnetic Beads</a></li><li><a href="/sg/en/home/brands/product-brand/dynal/dynabeads-magnetic-separation-products-selection-guide.html">Dynabeads Products Selection Guide </a></li></ul></li></ul></div></div></div></div></div><div class="railParsys parsys"></div></div></div><div class="span9 main" role="main"><div class="MainParsys parsys"><div class="heading section"><h2> Streptavidin-biotin binding process </h2></div><div class="text parbase section"><p>Streptavidin is a protein that is used extensively in molecular biology applications. Due to its exceptionally high affinity to biotin, it is among the strongest non-covalent interactions in nature. The small size of biotin makes it a popular choice for protein and cell labeling. Dynabeads streptavidin magnetic beads utilize this strong interaction to isolate cell populations, isolate target proteins, capture nucleic acids, synthesize mRNA, and prepare samples for next-generation sequencing (NGS) workflows. Learn more about the applications of using Dynabeads streptavidin beads.</p></div><div class="heading section"><div class="h2"> Explore the page: </div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt4"><div class="parsys_column cq-colctrl-lt4-c0"><div container-id="cq-colctrl-lt4" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_6537"><a href="#selection-guide"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_6537/foregroundimg.img.full.high.jpg/1730136161990.jpg' media="(min-width: 1px)"><img class='image-contain' srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_6537/foregroundimg.img.full.high.jpg/1730136161990.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_6537/foregroundimg.img.320.high.jpg/1730136161990.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_6537/foregroundimg.img.480.high.jpg/1730136161990.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_6537/foregroundimg.img.320.medium.jpg/1730136161990.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_6537/foregroundimg.img.620.high.jpg/1730136161990.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_6537/foregroundimg.img.320.low.jpg/1730136161990.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_6537/foregroundimg.img.480.medium.jpg/1730136161990.jpg" alt='Table icon' title='Selection guide'></picture><style></style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_6537 .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_6537 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div><div container-id="cq-colctrl-lt4" comp-id="A2"><div class="text parbase section"><p style="text-align: center;"><a href="#selection-guide"><b>Selection guide</b></a></p></div></div></div><div class="parsys_column cq-colctrl-lt4-c1"><div container-id="cq-colctrl-lt4" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_e50a"><a href="?open=target-enrichment#target-enrichment"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_e50a/foregroundimg.img.full.high.jpg/1730136162141.jpg' media="(min-width: 1px)"><img class='image-contain' srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_e50a/foregroundimg.img.full.high.jpg/1730136162141.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_e50a/foregroundimg.img.320.high.jpg/1730136162141.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_e50a/foregroundimg.img.480.high.jpg/1730136162141.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_e50a/foregroundimg.img.320.medium.jpg/1730136162141.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_e50a/foregroundimg.img.620.high.jpg/1730136162141.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_e50a/foregroundimg.img.320.low.jpg/1730136162141.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_e50a/foregroundimg.img.480.medium.jpg/1730136162141.jpg" alt='Targeted sequencing icon' title='Target enrichment for NGS '></picture><style></style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_e50a .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_e50a .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div><div container-id="cq-colctrl-lt4" comp-id="B2"><div class="text parbase section"><p style="text-align: center;"><a href="?open=target-enrichment#target-enrichment"><b>Target enrichment for NGS</b></a></p></div></div></div><div class="parsys_column cq-colctrl-lt4-c2"><div container-id="cq-colctrl-lt4" comp-id="C1"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_d526"><a href="open=mrna-synthesis#mrna-synthesis"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_d526/foregroundimg.img.full.high.png/1730136162281.png' media="(min-width: 1px)"><img class='image-contain' srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_d526/foregroundimg.img.full.high.png/1730136162281.png,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_d526/foregroundimg.img.320.high.png/1730136162281.png,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_d526/foregroundimg.img.480.high.png/1730136162281.png,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_d526/foregroundimg.img.320.medium.png/1730136162281.png,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_d526/foregroundimg.img.620.high.png/1730136162281.png,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_d526/foregroundimg.img.320.low.png/1730136162281.png,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_d526/foregroundimg.img.480.medium.png/1730136162281.png" alt='mRNA icon' title='mRNA synthesis by in vitro transcription '></picture><style></style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_d526 .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_d526 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div><div container-id="cq-colctrl-lt4" comp-id="C2"><div class="text parbase section"><p style="text-align: center;"><b><a href="?open=mrna-synthesis#mrna-synthesis">mRNA synthesis by <i>in vitro </i>transcription (IVT)</a></b></p></div></div></div><div class="parsys_column cq-colctrl-lt4-c3"><div container-id="cq-colctrl-lt4" comp-id="D1"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_9868"><a href="?open=protein-cell-capture#protein-cell-capture"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_9868/foregroundimg.img.full.high.jpg/1730136162415.jpg' media="(min-width: 1px)"><img class='image-contain' srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_9868/foregroundimg.img.full.high.jpg/1730136162415.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_9868/foregroundimg.img.320.high.jpg/1730136162415.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_9868/foregroundimg.img.480.high.jpg/1730136162415.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_9868/foregroundimg.img.320.medium.jpg/1730136162415.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_9868/foregroundimg.img.620.high.jpg/1730136162415.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_9868/foregroundimg.img.320.low.jpg/1730136162415.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_9868/foregroundimg.img.480.medium.jpg/1730136162415.jpg" alt='Dynabeads icon' title='Protein and cell capture'></picture><style></style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_9868 .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_9868 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div><div container-id="cq-colctrl-lt4" comp-id="D2"><div class="text parbase section"><p style="text-align: center;"><a href="?open=protein-cell-capture#protein-cell-capture"><b>Protein and cell capture</b></a></p></div></div></div></div><div style="clear:both"></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt4"><div class="parsys_column cq-colctrl-lt4-c0"><div container-id="cq-colctrl-lt4" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_34a4"><a href="#faqs"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_34a4/foregroundimg.img.full.high.jpg/1730136162620.jpg' media="(min-width: 1px)"><img class='image-contain' srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_34a4/foregroundimg.img.full.high.jpg/1730136162620.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_34a4/foregroundimg.img.320.high.jpg/1730136162620.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_34a4/foregroundimg.img.480.high.jpg/1730136162620.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_34a4/foregroundimg.img.320.medium.jpg/1730136162620.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_34a4/foregroundimg.img.620.high.jpg/1730136162620.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_34a4/foregroundimg.img.320.low.jpg/1730136162620.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_34a4/foregroundimg.img.480.medium.jpg/1730136162620.jpg" alt='Circle help icon' title='Streptavidin bead FAQs'></picture><style></style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_34a4 .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_34a4 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div><div container-id="cq-colctrl-lt4" comp-id="A2"><div class="text parbase section"><p style="text-align: center;"><b><a href="#faqs">Streptavidin bead FAQs</a></b></p></div></div></div><div class="parsys_column cq-colctrl-lt4-c1"><div container-id="cq-colctrl-lt4" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_c976"><a href="?open=publications#publications"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_c976/foregroundimg.img.full.high.jpg/1730136162763.jpg' media="(min-width: 1px)"><img class='image-contain' srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_c976/foregroundimg.img.full.high.jpg/1730136162763.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_c976/foregroundimg.img.320.high.jpg/1730136162763.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_c976/foregroundimg.img.480.high.jpg/1730136162763.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_c976/foregroundimg.img.320.medium.jpg/1730136162763.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_c976/foregroundimg.img.620.high.jpg/1730136162763.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_c976/foregroundimg.img.320.low.jpg/1730136162763.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_c976/foregroundimg.img.480.medium.jpg/1730136162763.jpg" alt='Book technical documentation icon' title='Publications using Streptavidin beads'></picture><style></style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_c976 .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_c976 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div><div container-id="cq-colctrl-lt4" comp-id="B2"><div class="text parbase section"><p style="text-align: center;"><a href="?open=publications#publications"><b>Publications using Streptavidin beads</b></a></p></div></div></div><div class="parsys_column cq-colctrl-lt4-c2"><div container-id="cq-colctrl-lt4" comp-id="C1"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_b090"><a href="#order"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_b090/foregroundimg.img.full.high.jpg/1730136162924.jpg' media="(min-width: 1px)"><img class='image-contain' srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_b090/foregroundimg.img.full.high.jpg/1730136162924.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_b090/foregroundimg.img.320.high.jpg/1730136162924.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_b090/foregroundimg.img.480.high.jpg/1730136162924.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_b090/foregroundimg.img.320.medium.jpg/1730136162924.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_b090/foregroundimg.img.620.high.jpg/1730136162924.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_b090/foregroundimg.img.320.low.jpg/1730136162924.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_b090/foregroundimg.img.480.medium.jpg/1730136162924.jpg" alt='Cart outline icon' title='Order Streptavidin magnetic beads'></picture><style></style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_b090 .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_b090 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div><div container-id="cq-colctrl-lt4" comp-id="C2"><div class="text parbase section"><p style="text-align: center;"><a href="#order"><b>Order Streptavidin magnetic beads</b></a></p></div></div></div><div class="parsys_column cq-colctrl-lt4-c3"><div container-id="cq-colctrl-lt4" comp-id="D1"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_dad1"><a href="#resources"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_dad1/foregroundimg.img.full.high.jpg/1730136163043.jpg' media="(min-width: 1px)"><img class='image-contain' srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_dad1/foregroundimg.img.full.high.jpg/1730136163043.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_dad1/foregroundimg.img.320.high.jpg/1730136163043.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_dad1/foregroundimg.img.480.high.jpg/1730136163043.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_dad1/foregroundimg.img.320.medium.jpg/1730136163043.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_dad1/foregroundimg.img.620.high.jpg/1730136163043.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_dad1/foregroundimg.img.320.low.jpg/1730136163043.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/image_dad1/foregroundimg.img.480.medium.jpg/1730136163043.jpg" alt='Book catalog icon' title='Learning resources'></picture><style></style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_dad1 .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsysimage_dad1 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div><div container-id="cq-colctrl-lt4" comp-id="D2"><div class="text parbase section"><p style="text-align: center;"><a href="#resources"><b>Learning resources</b></a></p></div></div></div></div><div style="clear:both"></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="well-cmp"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><div class="h2"> Selection guide for Dynabeads products </div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><p>Dynabeads products are available for a multitude of molecular and cellular applications. Don’t know where to start? Use our easy selection guide that considers sample type, target molecule, and downstream application to provide tailored recommendations for Dynabeads products.</p><p><a class="cta-link" href="/sg/en/home/brands/product-brand/dynal/dynabeads-magnetic-separation-products-selection-guide.html">Dynabeads product selection guide</a></p></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-fd598175-dace-4391-9fef-15325671dabcMainParsyswell_8fa1uiparimage_44ab"><a href="/sg/en/home/brands/product-brand/dynal/dynabeads-magnetic-separation-products-selection-guide.html"><picture><source srcset='https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.320.high.jpg/1710339445247.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.480.high.jpg/1710339445247.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.full.high.jpg/1710339445247.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.320.high.jpg/1710339445247.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.480.high.jpg/1710339445247.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.320.medium.jpg/1710339445247.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.620.high.jpg/1710339445247.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.320.low.jpg/1710339445247.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.480.medium.jpg/1710339445247.jpg" alt='Conceptual magnetic beads, antibodies, and DNA' title='Dynabeads Products Selection Guide'></picture><style> #cq-image-jsp-fd598175-dace-4391-9fef-15325671dabcMainParsyswell_8fa1uiparimage_44ab img{ width:100%; max-width: 525px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"fd598175-dace-4391-9fef-15325671dabcMainParsyswell_8fa1uiparimage_44ab .image-contain" + "," + "#cq-image-jsp-"+"fd598175-dace-4391-9fef-15325671dabcMainParsyswell_8fa1uiparimage_44ab .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div><div class="heading section"><h2 id="selection-guide"> Dynabeads streptavidin bead selection table </h2></div><div class="heading section"><h3> Table 1. Streptavidin beads by target and application </h3></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-104560803"><tbody><tr><th>Target</th><th>Typical applications</th><th width="35%">Recommended products</th></tr><tr><td><h4>Nucleic acid capture</h4><img src="/content/dam/LifeTech/global/life-sciences/images/applications-fig1.png" title="Nucleic Acid capture" alt="Illustration of a magnetic bead bound to a single-stranded nucleic acid fragment"></td><td><ul><li><a href="?target-enrichment#target-enrichment">NGS workflows</a></li><li>Sequence-specific capture</li><li>DNA or RNA templating</li></ul></td><td><ul><li><a href="/order/catalog/product/65605D">Dynabeads Streptavidin for Target Enrichment</a></li><li><a href="/order/catalog/product/65305">Dynabeads M-270 Streptavidin</a></li><li><a href="/order/catalog/product/65001">Dynabeads MyOne Streptavidin C1</a></li><li><a href="/order/catalog/product/60101">Dynabeads kilobaseBINDER Kit</a></li></ul></td></tr><tr><td><h4>Protein isolation</h4><img src="/content/dam/LifeTech/global/life-sciences/images/applications-fig5.png" title="Protein capture" alt="Illustration of a magnetic bead bound to a protein ligand"></td><td><ul><li><a href="?open=protein-cell-capture#protein-cell-capture">Protein and peptide purification</a></li><li>Immunoassays</li><li>Phage display</li></ul></td><td><ul><li><a href="/order/catalog/product/11205D">Dynabeads M-280 Streptavidin</a></li><li><a href="/order/catalog/product/65601">Dynabeads MyOne Streptavidin T1</a></li></ul></td></tr><tr><td><h4>Cell isolation</h4><img src="/content/dam/LifeTech/global/life-sciences/images/applications-fig7.png" title="Cell capture" alt="Illustration of a magnetic bead bound to a cell surface ligand"></td><td><ul><li><a href="?open=protein-cell-capture#protein-cell-capture">Cell purification</a></li><li>Pathogen detection</li><li>Exosome isolation</li></ul></td><td><ul><li><a href="/order/catalog/product/11205D">Dynabeads M-280 Streptavidin</a></li><li><a href="/order/catalog/product/65601">Dynabeads MyOne Streptavidin T1</a></li><li><a href="/order/catalog/product/11047">Dynabeads Biotin Binder</a></li><li><a href="/order/catalog/product/11533D">CELLection Biotin Binder Kit</a></li><li><a href="/order/catalog/product/11061D">Dynabeads FlowComp Flexi</a></li></ul></td></tr></tbody></table></div></div><div class="tabworkflowcontainer section"><div class="tabbable plain-tab"><ul class="nav nav-tabs" id="myTab"><li class="active"><a data-toggle="tab" href="#container-5d6811503987711">NGS</a></li><li><a data-toggle="tab" href="#container-5d6811503987712">mRNA</a></li><li><a data-toggle="tab" href="#container-5d6811503987713">Protein and cells</a></li></ul><div class="tab-content" id="myTabContent"><div id="container-5d6811503987711" class="tab-pane active"><div class="container-5d6811503987711 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2><br>Streptavidin beads for target sequence enrichment </h2></div><div class="text parbase section"><p>NGS as a high-throughput, massive parallel sequencing technology enables the sequencing of DNA and RNA for studying genetic variations associated with diseases and other biological processes. Applications include whole genome sequencing, deep sequence of target regions, and identification of new pathogens to further advance <a href="/sg/en/home/life-science/sequencing/dna-sequencing/microbial-sequencing/microbial-identification-ion-torrent-next-generation-sequencing.html">infectious disease and microbial research</a>.</p><p>The <a href="/sg/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/next-generation-sequencing/illumina-workflow.html">four key steps for NGS workflow</a> generally include library preparation, clonal amplification, library sequencing, and data analysis. <a href="?open=seq#seq">Dynabeads Streptavidin for Target Enrichment</a> is excellent for the enrichment of biotinylated target nucleic acid sequences made during the library prep as part of the NGS workflow (<b>Figure 1</b>).</p></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabimage_815d"><a class="image-enlarge-link" data-lightbox="image_815d" href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/_jcr_content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_815d/backgroundimg.img.jpg/1730136163878.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_815d/foregroundimg.img.320.high.jpg/1730136163808.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_815d/foregroundimg.img.480.high.jpg/1730136163808.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_815d/foregroundimg.img.full.high.jpg/1730136163808.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_815d/foregroundimg.img.320.high.jpg/1730136163808.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_815d/foregroundimg.img.480.high.jpg/1730136163808.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_815d/foregroundimg.img.320.medium.jpg/1730136163808.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_815d/foregroundimg.img.620.high.jpg/1730136163808.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_815d/foregroundimg.img.320.low.jpg/1730136163808.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_815d/foregroundimg.img.480.medium.jpg/1730136163808.jpg" alt='Schematic showing the NGS workflow starting with 1) library preparation, 2) hybridization, 3) target enrichment, 4) PCR, and 5) sequencing' title='Target enrichment in the NGS workflow'></picture><style> #cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabimage_815d img{ width:100%; max-width: 600px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabimage_815d .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabimage_815d .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="text parbase section"><p><small><b>Figure 1. The NGS workflow.</b> The first step for NGS is library preparation, followed by hybridization, target enrichment, PCR, and sequencing. Dynabeads Streptavidin for Target Enrichment is intended for the enrichment of the target sequences hybridized on the biotinylated probe that is immobilized to the streptavidin-coated magnetic beads (shown in red). The hybridization step can either be performed prior to the bead-based target enrichment step (indirect hybrid capture technique) or after the biotinylated probe is bound to the streptavidin beads (direct hybrid capture technique).</small></p></div></div></div><div style="clear:both"></div><div class="heading section"><h3> Bead-based target enrichment for NGS </h3></div><div class="text parbase section"><p><a href="/sg/en/home/global/forms/life-science/resources-bead-based-target-enrichment-ngs.html" class="btn btn-primary">Watch webinar</a></p><p>Target enrichment by <b>hybridization</b> allows capture and therefore sufficient coverage of genomic regions from degraded sample types whether using DNA, RNA, or circulating cell-free DNA from blood. The biotinylated probes (also known as bait) immobilize the oligonucleotides onto the bead surface by binding with 1 μm in diameter streptavidin beads (<b>Figure 2</b>). The isolated and purified target regions are subsequently amplified and prepared for sequencing.</p><p>Dynabeads Streptavidin for Target Enrichment provides the following advantages:</p><ul><li><b>High purity</b>—helps minimize non-specific binding</li><li><b>High reproducibility</b>—helps achieve consistent results and can help reduce the risk of failure</li><li><b>High sensitivity</b>—secures successful NGS workflow</li><li><b>Automation</b>—enables high-throughput enrichment</li></ul></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabimage_a061"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_a061/foregroundimg.img.320.high.png/1730136164105.png' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_a061/foregroundimg.img.480.high.png/1730136164105.png' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_a061/foregroundimg.img.full.high.png/1730136164105.png,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_a061/foregroundimg.img.320.high.png/1730136164105.png,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_a061/foregroundimg.img.480.high.png/1730136164105.png,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_a061/foregroundimg.img.320.medium.png/1730136164105.png,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_a061/foregroundimg.img.620.high.png/1730136164105.png,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_a061/foregroundimg.img.320.low.png/1730136164105.png,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_a061/foregroundimg.img.480.medium.png/1730136164105.png" alt='Diagrammed steps of nucleic acid extraction using Dynabeads streptavidin' title='Hybridization capture with streptavidin beads '></picture><style> #cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabimage_a061 img{ width:100%; max-width: 600px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabimage_a061 .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabimage_a061 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="text parbase section"><p><small><b>Figure 2. Hybridization capture with streptavidin beads.</b> Streptavidin beads immobilize the biotinylated ligand and use it as bait to capture target nucleic acid sequences.</small></p></div></div></div><div style="clear:both"></div><div class="text parbase section"><p><a class="cta-link" href="?open=seq#seq">Order Dynabeads Streptavidin for Target Enrichment</a></p></div><div class="accordion section"><div class="accordion" id="85d8b80b904f4e97adc9d524b2dc3fd2" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#85d8b80b904f4e97adc9d524b2dc3fd2" href="#benchmarking"><span class="caret"></span> Benchmarking data for Dynabeads streptavidin beads in the NGS workflow </a></div><div class="accordion-body collapse " id="benchmarking"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="heading section"><h3> Performance of Dynabeads Streptavidin for Target Enrichment in a sequencing workflow vs comparable products </h3></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_6569"><a class="image-enlarge-link" data-lightbox="image_6569" href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/_jcr_content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/backgroundimg.img.jpg/1730136164739.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.320.low.jpg/1730136164679.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.320.medium.jpg/1730136164679.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.480.medium.jpg/1730136164679.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.620.high.jpg/1730136164679.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.full.high.jpg/1730136164679.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.full.high.jpg/1730136164679.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.320.high.jpg/1730136164679.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.480.high.jpg/1730136164679.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.320.medium.jpg/1730136164679.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.620.high.jpg/1730136164679.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.320.low.jpg/1730136164679.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.480.medium.jpg/1730136164679.jpg" alt='Graph showing the mean concentration of final libraries using Dynabeads Streptavidin for Target Enrichment and four other vendors' title='Streptavidin magnetic beads comparison: mean concentration of final libraries'></picture><style> #cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_6569 img{ width:100%; max-width: 1494px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_6569 .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_6569 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><p><small><b>Figure 3. Streptavidin magnetic beads comparison: mean concentration of final libraries.</b> We measured concentrations (nM) of final libraries for Dynabeads Streptavidin for Target Enrichment (red bar), a positive control (gray bar), and streptavidin magnetic beads from four other vendors. The concentration of final libraries was calculated based on the concentration of the library fragments and the size distribution. For downstream exome sequencing, the acceptance criteria was set to 2.5 nM. n=3.</small></p></div></div></div></div><div style="clear:both"></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_f04f"><a class="image-enlarge-link" data-lightbox="image_f04f" href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/_jcr_content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/backgroundimg.img.jpg/1730136164906.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.320.low.jpg/1730136164843.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.320.medium.jpg/1730136164843.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.480.medium.jpg/1730136164843.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.620.high.jpg/1730136164843.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.full.high.jpg/1730136164843.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.full.high.jpg/1730136164843.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.320.high.jpg/1730136164843.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.480.high.jpg/1730136164843.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.320.medium.jpg/1730136164843.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.620.high.jpg/1730136164843.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.320.low.jpg/1730136164843.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.480.medium.jpg/1730136164843.jpg" alt='Graph showing the mean coverage of libraries using Dynabeads Streptavidin for Target Enrichment and two other vendors' title='Streptavidin magnetic beads comparison: mean coverage'></picture><style> #cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_f04f img{ width:100%; max-width: 1464px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_f04f .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_f04f .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><p><small><b>Figure 4. Streptavidin magnetic beads comparison: mean coverage.</b> Mean sequencing coverage for Dynabeads Streptavidin for Target Enrichment (red bar), a positive control (gray bar), and streptavidin magnetic beads from the two vendors that passed the target enrichment concentration criteria outlined in Figure 3. The mean coverage is expressed as an average of the triplicates, all samples were prepared in triplicates (n=3). The error bar is the standard deviation. Dynabeads Streptavidin for Target Enrichment gave the deepest coverage (most reads from which to build a consensus and the greatest accuracy). </small></p></div></div></div></div><div style="clear:both"></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_b88d"><a class="image-enlarge-link" data-lightbox="image_b88d" href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/_jcr_content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/backgroundimg.img.jpg/1730136165104.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.320.low.jpg/1730136165042.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.320.medium.jpg/1730136165042.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.480.medium.jpg/1730136165042.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.620.high.jpg/1730136165042.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.full.high.jpg/1730136165042.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.full.high.jpg/1730136165042.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.320.high.jpg/1730136165042.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.480.high.jpg/1730136165042.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.320.medium.jpg/1730136165042.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.620.high.jpg/1730136165042.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.320.low.jpg/1730136165042.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.480.medium.jpg/1730136165042.jpg" alt='Graph comparing the average depth of coverage of libraries using Dynabeads Streptavidin for Target Enrichment and two other vendors ' title='Streptavidin magnetic beads comparison: average depth of coverage'></picture><style> #cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_b88d img{ width:100%; max-width: 1545px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_b88d .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_b88d .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><p><small><b>Figure 5. Streptavidin magnetic beads comparison: average depth of coverage.</b> The % of reference sequence covered vs the coverage depth for Dynabeads Streptavidin for Target Enrichment (red bars), a positive control (gray bars), and streptavidin magnetic beads from the two vendors that passed the target enrichment concentration criteria outlined in Figure 3. For Dynabeads Streptavidin for Target Enrichment at 60x, 97.53% of the reference is covered with a minimum of 60 reads. This performance of the Dynabeads Streptavidin for Target Enrichment is particularly important for clinical applications and identification of rare variants where deep coverage (e.g., 60x) is essential.</small></p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#85d8b80b904f4e97adc9d524b2dc3fd2').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#85d8b80b904f4e97adc9d524b2dc3fd2').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div></div><div class="end"></div></div></div><div id="container-5d6811503987712" class="tab-pane"><div class="container-5d6811503987712 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2><br>Streptavidin beads for mRNA synthesis </h2></div><div class="text parbase section"><p>Streptavidin beads can be used for mRNA synthesis by <i>in vitro</i> transcription (IVT), an application/workflow that can greatly accelerate the research and development of mRNA vaccines. The mRNA is synthesized using a biotinylated DNA template of choice, amplified via PCR, and immobilized to streptavidin beads (<b>Figure 6</b>). The immobilized template can be reused in the next IVT reaction at least six times.</p></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="text parbase section"><p><small><b>Figure 6. mRNA synthesis workflow with Dynabeads Streptavidin for <i>In Vitro</i> Transcription.</b> This workflow begins with the biotinylated PCR product. This mRNA template is then immobilized to the <a href="?open=mrna#mrna">Dynabeads Streptavidin for <i>In Vitro</i> Transcription</a> beads forming a bead-template complex. Using this complex, IVT can be performed up to six consecutive times. The target mRNA is then ready to be eluted from the streptavidin beads via magnetic separation and incubation with elution buffer.</small></p></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987712tabimage_8da2"><a class="image-enlarge-link" data-lightbox="image_8da2" href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/_jcr_content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_8da2/backgroundimg.img.jpg/1730136165530.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_8da2/foregroundimg.img.320.high.jpg/1730136165473.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_8da2/foregroundimg.img.480.high.jpg/1730136165473.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_8da2/foregroundimg.img.full.high.jpg/1730136165473.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_8da2/foregroundimg.img.320.high.jpg/1730136165473.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_8da2/foregroundimg.img.480.high.jpg/1730136165473.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_8da2/foregroundimg.img.320.medium.jpg/1730136165473.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_8da2/foregroundimg.img.620.high.jpg/1730136165473.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_8da2/foregroundimg.img.320.low.jpg/1730136165473.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_8da2/foregroundimg.img.480.medium.jpg/1730136165473.jpg" alt='Schematic showing the mRNA synthesis workflow starting with the biotinylated PCR product, then immobilization of target mRNA to streptavidin beads, then in vitro transcription when mRNA target is enriched' title='mRNA synthesis workflow using streptavidin magnetic beads'></picture><style> #cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987712tabimage_8da2 img{ width:100%; max-width: 600px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987712tabimage_8da2 .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987712tabimage_8da2 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div></div><div style="clear:both"></div><div class="heading section"><h3> mRNA synthesis by <i>in vitro</i> transcription (IVT) </h3></div><div class="text parbase section"><p>mRNA synthesis by IVT begins with the biotinylated template, which is made by PCR amplification of the target sequence in a plasmid construct or in a synthetic DNA construct using a biotinylated forward primer located 30–100 bps upstream of the T7-promoter and a non-biotinylated reverse primer (<b>Figure 7</b>). The biotinylated template is immobilized to the streptavidin beads without the need for prior purification. The streptavidin bead-bound template is used directly in IVT, then removed from the synthesized mRNA by magnetic separation. The isolated and purified target regions are subsequently amplified and prepared for sequencing.</p><p><b>Dynabeads Streptavidin for <i>In Vitro</i> Transcription</b> is designed for simple and scalable mRNA synthesis. Product benefits include:</p><ul><li><b>Reusability</b>—reuse the DNA template at least six times to minimize plasmid preparation and reduce hands-on time</li><li><b>Flexibility</b>—choose synthetic or plasmid DNA template for mRNA IVT for greater flexibility of mRNA design</li><li><b>Automation</b>— enables high-throughput synthesis and purification of many samples simultaneously</li></ul></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="text parbase section"><p><small><b>Figure 7. mRNA synthesis by IVT using Dynabeads Streptavidin for <i>In Vitro</i> Transcription.</b> The biotinylated DNA template is immobilized to the streptavidin magnetic beads and contains an upstream linker region, T7 promoter to facilitate binding of the T7 RNA polymerase, and an mRNA coding region. mRNA synthesis can be done up to six times using the same DNA template.</small></p></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987712tabimage_3627"><a class="image-enlarge-link" data-lightbox="image_3627" href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/_jcr_content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/backgroundimg.img.jpg/1730136165741.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.320.low.jpg/1730136165672.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.320.medium.jpg/1730136165672.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.480.medium.jpg/1730136165672.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.620.high.jpg/1730136165672.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.full.high.jpg/1730136165672.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.320.high.jpg/1730136165672.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.480.high.jpg/1730136165672.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.320.medium.jpg/1730136165672.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.620.high.jpg/1730136165672.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.320.low.jpg/1730136165672.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.480.medium.jpg/1730136165672.jpg" alt='Diagram of how mRNA synthesis with Dynabeads Streptavidin for In Vitro Transcription works' title='mRNA synthesis by IVT using Dynabeads Streptavidin for In Vitro Transcription'></picture><style> #cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987712tabimage_3627 img{ width:100%; max-width: 949px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987712tabimage_3627 .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987712tabimage_3627 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><p><a class="cta-link" href="/sg/en/home/industrial/pharma-biopharma/nucleic-acid-therapeutic-development-solutions/mrna-therapeutic-resources/mrna-synthesis-reagents/magnetic-beads-mrna-production.html">Read more about magnetic bead-based solutions for mRNA synthesis</a><br><a class="cta-link" href="?open=mrna#mrna">Order Dynabeads Streptavidin for <i>In Vitro</i> Transcription now</a></p></div></div><div class="end"></div></div></div><div id="container-5d6811503987713" class="tab-pane"><div class="container-5d6811503987713 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2><br>Streptavidin beads for protein and cell capture </h2></div><div class="text parbase section"><p>When used in combination with a biotinylated probe/ligand, any target molecule can be captured on the Dynabeads streptavidin magnetic beads, isolated and then further manipulated. Depending on your target molecule and downstream assay, a variety of products are available for protein and cell capture using streptavidin magnetic beads. View <b>Table 2</b> below to determine which product meets your specific research needs.<br>  </p></div><div class="heading section"><h3> Table 2. Streptavidin magnetic beads for direct and indirect isolation </h3></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--1309989672"><tbody><tr><th style="width: 15%;">Recommended products</th><th style="width: 15%;"><a></a><a href="/order/catalog/product/11205D">Dynabeads M-280 Streptavidin</a></th><th style="width: 15%;"><a></a><a href="/order/catalog/product/65601">Dynabeads MyOne Streptavidin T1</a></th><th style="width: 15%;"><a></a><a href="/order/catalog/product/11047">Dynabeads Biotin Binder</a></th><th style="width: 15%;"><a></a><a href="/order/catalog/product/11533D">CELLection Biotin Binder Kit</a></th><th style="width: 15%;"><a></a><a href="/order/catalog/product/11061D">Dynabeads FlowComp Flexi Kit</a></th></tr><tr><td><b>Description</b></td><td><ul><li>Hydrophobic bead surface</li><li>Based on tosylactivated beads</li><li>Diameter: 2.8 µm</li><li>Size distribution:<br> CV < 3%</li><li>BSA as blocking protein</li><li>Isoelectric point: pH 5.0</li><li>Low charge (–10 mV (at pH 7)</li><li>Iron content (Ferrites): 12% (17%)</li></ul></td><td><ul><li>Hydrophobic bead surface</li><li>Based on tosylactivated beads</li><li>Diameter: 1.05 µm</li><li>Size distribution: CV <3%</li><li>BSA as blocking protein</li><li>Isoelectric point: pH 5.0</li><li>Low charge (–10 mV (at pH 7)</li><li>Iron content (Ferrites): 26% (37%)</li><li>Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads</li></ul></td><td><ul><li>For use with your own standard biotinylated antibodies</li><li>Isolate cells from whole blood, buffy coat, PBMC, MNC, or tissue digests</li></ul></td><td><ul><li>For use with your own standard biotinylated antibodies</li><li>Streptavidin on the bead-surface is attached via a DNA linker, providing a cleavable site to release and remove the beads after isolation</li></ul></td><td><ul><li>For use with your own standard biotinylated antibodies</li><li>DSB-X biotinylation of the antibody is required to assure release of the target cells from the bead</li></ul></td></tr><tr><td><b>Capacity</b></td><td><ul><li>Free biotin:<br> 490–750<br> pmol/mg beads</li><li>Biotinylated<br> Ig:<br> 5–10 µg/mg<br> beads</li></ul></td><td><ul><li>Free biotin:<br> 950–1,500 pmol/mg beads</li><li>Biotinylated Ig:<br> 20 µg/mg beads</li></ul></td><td>Processes 2 x 10<sup>9</sup> PBMCs or 200 mL whole blood</td><td>Processes 2 x 10<sup>9</sup> PBMCs or 200 mL whole blood</td><td>Processes 2 x 10<sup>9</sup> MNCs or 80 mL whole blood/buffy coat</td></tr><tr><td><b>Ideal applications</b></td><td><ul><li>Immunoassays/<br> Immunodiagnostics (for research use only)</li><li>Protein purification</li><li>Phage display</li><li>Biopanning</li><li>Cell isolation</li></ul></td><td><ul><li>Immunoassays/<br> Immunodiagnostics (for research use only)</li><li>Protein purification</li><li>Phage display</li><li>Biopanning</li><li>Cell isolation</li><li>Automation</li></ul></td><td><ul><li>Depletion of one/multiple cell types from any sample from any species</li><li>Positive cell isolation for molecular downstream applications (without cell release)</li></ul></td><td><ul><li>Positive cell isolation and release</li><li>Isolate any cell from any sample from any species</li></ul></td><td><ul><li>Positive cell isolation and release</li><li>Isolate any cell from any sample from any species</li></ul></td></tr></tbody></table></div></div><div class="heading section"><h3><br>Direct and indirect capture of molecules and cells with streptavidin beads </h3></div><div class="parsys_column cq-colctrl-lt8"><div class="parsys_column cq-colctrl-lt8-c0"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987713tabimage_fdac"><a class="image-enlarge-link" data-lightbox="image_fdac" href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/_jcr_content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987713/tab/image_fdac/backgroundimg.img.gif/1730136166149.gif"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987713/tab/image_fdac/foregroundimg.img.full.high.gif/1730136166079.gif' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987713/tab/image_fdac/foregroundimg.img.full.high.gif/1730136166079.gif,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987713/tab/image_fdac/foregroundimg.img.320.high.gif/1730136166079.gif,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987713/tab/image_fdac/foregroundimg.img.480.high.gif/1730136166079.gif,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987713/tab/image_fdac/foregroundimg.img.320.medium.gif/1730136166079.gif,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987713/tab/image_fdac/foregroundimg.img.620.high.gif/1730136166079.gif,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987713/tab/image_fdac/foregroundimg.img.320.low.gif/1730136166079.gif,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987713/tab/image_fdac/foregroundimg.img.480.medium.gif/1730136166079.gif" alt='Diagram detailing direct capture versus indirect capture of molecules of cells using streptavidin magnetic beads; direct capture uses a pre-coupled ligand that is added to the sample, incubated, then eluted via magnetic separation and indirect capture begins with incubating the biotinylated probe/ligand to the sample then adding the streptavidin magnetic beads, then eluting via magnetic separation' title='Streptavidin magnetic beads for direct and indirect capture of molecules and cells'></picture><style> #cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987713tabimage_fdac img{ width:100%; max-width: 275px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987713tabimage_fdac .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987713tabimage_fdac .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div class="text parbase section"><p>Depending on your target molecule and the specific application, the direct or indirect capture method can be applied using streptavidin magnetic beads (<b>Figure 8</b>).</p><p>For some applications, using a pre-coupled ligand in direct capture allows you to reuse the magnetic beads and thus further reduce sample preparation costs.</p><p>The indirect capture approach can be of benefit when the concentration of your target is low, the specific affinity is weak, or the binding kinetics is slow. In indirect capture, the probe/ligand is allowed to bind to the target in suspension prior to addition of the beads. A monolayer of streptavidin is covalently coupled to the magnetic beads, helping to reduce leakage that could otherwise disturb your assay.</p><p><small><b>Figure 8. Direct and indirect capture of molecules and cells with streptavidin magnetic beads.</b> Direct capture uses a pre-coupled ligand that is added to the sample, incubated, then eluted via magnetic separation. Indirect capture begins with incubation of the biotinylated probe/ligand to the sample, then the streptavidin magnetic beads are added and incubated for some time, followed by elution off the magnetic bead by magnetic separation.</small></p><p><a class="cta-link" href="?open=capture#capture">Order Dynabeads streptavidin beads for protein and cell capture now</a></p></div></div></div><div style="clear:both"></div></div><div class="end"></div></div></div></div></div><div class="workflow-accordion accordion" id="accordion-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771"><div class="accordion-group"><div class="accordion-heading active"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771" href="#collapse1"><span class="caret"></span><strong> NGS </strong></a></div><div id="collapse1" class="accordion-body collapse in "><div class="accordion-inner container-5d6811503987711"><div class="container-5d6811503987711 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2><br>Streptavidin beads for target sequence enrichment </h2></div><div class="text parbase section"><p>NGS as a high-throughput, massive parallel sequencing technology enables the sequencing of DNA and RNA for studying genetic variations associated with diseases and other biological processes. Applications include whole genome sequencing, deep sequence of target regions, and identification of new pathogens to further advance <a href="/sg/en/home/life-science/sequencing/dna-sequencing/microbial-sequencing/microbial-identification-ion-torrent-next-generation-sequencing.html">infectious disease and microbial research</a>.</p><p>The <a href="/sg/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/next-generation-sequencing/illumina-workflow.html">four key steps for NGS workflow</a> generally include library preparation, clonal amplification, library sequencing, and data analysis. <a href="?open=seq#seq">Dynabeads Streptavidin for Target Enrichment</a> is excellent for the enrichment of biotinylated target nucleic acid sequences made during the library prep as part of the NGS workflow (<b>Figure 1</b>).</p></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabimage_815d"><a class="image-enlarge-link" data-lightbox="image_815d" href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/_jcr_content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_815d/backgroundimg.img.jpg/1730136163878.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_815d/foregroundimg.img.320.high.jpg/1730136163808.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_815d/foregroundimg.img.480.high.jpg/1730136163808.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_815d/foregroundimg.img.full.high.jpg/1730136163808.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_815d/foregroundimg.img.320.high.jpg/1730136163808.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_815d/foregroundimg.img.480.high.jpg/1730136163808.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_815d/foregroundimg.img.320.medium.jpg/1730136163808.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_815d/foregroundimg.img.620.high.jpg/1730136163808.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_815d/foregroundimg.img.320.low.jpg/1730136163808.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_815d/foregroundimg.img.480.medium.jpg/1730136163808.jpg" alt='Schematic showing the NGS workflow starting with 1) library preparation, 2) hybridization, 3) target enrichment, 4) PCR, and 5) sequencing' title='Target enrichment in the NGS workflow'></picture><style> #cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabimage_815d img{ width:100%; max-width: 600px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabimage_815d .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabimage_815d .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="text parbase section"><p><small><b>Figure 1. The NGS workflow.</b> The first step for NGS is library preparation, followed by hybridization, target enrichment, PCR, and sequencing. Dynabeads Streptavidin for Target Enrichment is intended for the enrichment of the target sequences hybridized on the biotinylated probe that is immobilized to the streptavidin-coated magnetic beads (shown in red). The hybridization step can either be performed prior to the bead-based target enrichment step (indirect hybrid capture technique) or after the biotinylated probe is bound to the streptavidin beads (direct hybrid capture technique).</small></p></div></div></div><div style="clear:both"></div><div class="heading section"><h3> Bead-based target enrichment for NGS </h3></div><div class="text parbase section"><p><a href="/sg/en/home/global/forms/life-science/resources-bead-based-target-enrichment-ngs.html" class="btn btn-primary">Watch webinar</a></p><p>Target enrichment by <b>hybridization</b> allows capture and therefore sufficient coverage of genomic regions from degraded sample types whether using DNA, RNA, or circulating cell-free DNA from blood. The biotinylated probes (also known as bait) immobilize the oligonucleotides onto the bead surface by binding with 1 μm in diameter streptavidin beads (<b>Figure 2</b>). The isolated and purified target regions are subsequently amplified and prepared for sequencing.</p><p>Dynabeads Streptavidin for Target Enrichment provides the following advantages:</p><ul><li><b>High purity</b>—helps minimize non-specific binding</li><li><b>High reproducibility</b>—helps achieve consistent results and can help reduce the risk of failure</li><li><b>High sensitivity</b>—secures successful NGS workflow</li><li><b>Automation</b>—enables high-throughput enrichment</li></ul></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabimage_a061"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_a061/foregroundimg.img.320.high.png/1730136164105.png' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_a061/foregroundimg.img.480.high.png/1730136164105.png' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_a061/foregroundimg.img.full.high.png/1730136164105.png,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_a061/foregroundimg.img.320.high.png/1730136164105.png,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_a061/foregroundimg.img.480.high.png/1730136164105.png,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_a061/foregroundimg.img.320.medium.png/1730136164105.png,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_a061/foregroundimg.img.620.high.png/1730136164105.png,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_a061/foregroundimg.img.320.low.png/1730136164105.png,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/image_a061/foregroundimg.img.480.medium.png/1730136164105.png" alt='Diagrammed steps of nucleic acid extraction using Dynabeads streptavidin' title='Hybridization capture with streptavidin beads '></picture><style> #cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabimage_a061 img{ width:100%; max-width: 600px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabimage_a061 .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabimage_a061 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="text parbase section"><p><small><b>Figure 2. Hybridization capture with streptavidin beads.</b> Streptavidin beads immobilize the biotinylated ligand and use it as bait to capture target nucleic acid sequences.</small></p></div></div></div><div style="clear:both"></div><div class="text parbase section"><p><a class="cta-link" href="?open=seq#seq">Order Dynabeads Streptavidin for Target Enrichment</a></p></div><div class="accordion section"><div class="accordion" id="8d83cd5c5d7c41c6bf4bd08917238dd9" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#8d83cd5c5d7c41c6bf4bd08917238dd9" href="#benchmarking"><span class="caret"></span> Benchmarking data for Dynabeads streptavidin beads in the NGS workflow </a></div><div class="accordion-body collapse " id="benchmarking"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="heading section"><h3> Performance of Dynabeads Streptavidin for Target Enrichment in a sequencing workflow vs comparable products </h3></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_6569"><a class="image-enlarge-link" data-lightbox="image_6569" href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/_jcr_content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/backgroundimg.img.jpg/1730136164739.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.320.low.jpg/1730136164679.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.320.medium.jpg/1730136164679.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.480.medium.jpg/1730136164679.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.620.high.jpg/1730136164679.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.full.high.jpg/1730136164679.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.full.high.jpg/1730136164679.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.320.high.jpg/1730136164679.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.480.high.jpg/1730136164679.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.320.medium.jpg/1730136164679.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.620.high.jpg/1730136164679.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.320.low.jpg/1730136164679.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_6569/foregroundimg.img.480.medium.jpg/1730136164679.jpg" alt='Graph showing the mean concentration of final libraries using Dynabeads Streptavidin for Target Enrichment and four other vendors' title='Streptavidin magnetic beads comparison: mean concentration of final libraries'></picture><style> #cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_6569 img{ width:100%; max-width: 1494px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_6569 .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_6569 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><p><small><b>Figure 3. Streptavidin magnetic beads comparison: mean concentration of final libraries.</b> We measured concentrations (nM) of final libraries for Dynabeads Streptavidin for Target Enrichment (red bar), a positive control (gray bar), and streptavidin magnetic beads from four other vendors. The concentration of final libraries was calculated based on the concentration of the library fragments and the size distribution. For downstream exome sequencing, the acceptance criteria was set to 2.5 nM. n=3.</small></p></div></div></div></div><div style="clear:both"></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_f04f"><a class="image-enlarge-link" data-lightbox="image_f04f" href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/_jcr_content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/backgroundimg.img.jpg/1730136164906.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.320.low.jpg/1730136164843.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.320.medium.jpg/1730136164843.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.480.medium.jpg/1730136164843.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.620.high.jpg/1730136164843.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.full.high.jpg/1730136164843.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.full.high.jpg/1730136164843.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.320.high.jpg/1730136164843.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.480.high.jpg/1730136164843.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.320.medium.jpg/1730136164843.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.620.high.jpg/1730136164843.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.320.low.jpg/1730136164843.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_f04f/foregroundimg.img.480.medium.jpg/1730136164843.jpg" alt='Graph showing the mean coverage of libraries using Dynabeads Streptavidin for Target Enrichment and two other vendors' title='Streptavidin magnetic beads comparison: mean coverage'></picture><style> #cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_f04f img{ width:100%; max-width: 1464px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_f04f .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_f04f .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><p><small><b>Figure 4. Streptavidin magnetic beads comparison: mean coverage.</b> Mean sequencing coverage for Dynabeads Streptavidin for Target Enrichment (red bar), a positive control (gray bar), and streptavidin magnetic beads from the two vendors that passed the target enrichment concentration criteria outlined in Figure 3. The mean coverage is expressed as an average of the triplicates, all samples were prepared in triplicates (n=3). The error bar is the standard deviation. Dynabeads Streptavidin for Target Enrichment gave the deepest coverage (most reads from which to build a consensus and the greatest accuracy). </small></p></div></div></div></div><div style="clear:both"></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_b88d"><a class="image-enlarge-link" data-lightbox="image_b88d" href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/_jcr_content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/backgroundimg.img.jpg/1730136165104.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.320.low.jpg/1730136165042.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.320.medium.jpg/1730136165042.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.480.medium.jpg/1730136165042.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.620.high.jpg/1730136165042.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.full.high.jpg/1730136165042.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.full.high.jpg/1730136165042.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.320.high.jpg/1730136165042.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.480.high.jpg/1730136165042.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.320.medium.jpg/1730136165042.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.620.high.jpg/1730136165042.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.320.low.jpg/1730136165042.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987711/tab/accordion_5089/itemspar/accordionitem_4dea/itemParsys/image_b88d/foregroundimg.img.480.medium.jpg/1730136165042.jpg" alt='Graph comparing the average depth of coverage of libraries using Dynabeads Streptavidin for Target Enrichment and two other vendors ' title='Streptavidin magnetic beads comparison: average depth of coverage'></picture><style> #cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_b88d img{ width:100%; max-width: 1545px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_b88d .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987711tabaccordion_5089itemsparaccordionitem_4deaitemParsysimage_b88d .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><p><small><b>Figure 5. Streptavidin magnetic beads comparison: average depth of coverage.</b> The % of reference sequence covered vs the coverage depth for Dynabeads Streptavidin for Target Enrichment (red bars), a positive control (gray bars), and streptavidin magnetic beads from the two vendors that passed the target enrichment concentration criteria outlined in Figure 3. For Dynabeads Streptavidin for Target Enrichment at 60x, 97.53% of the reference is covered with a minimum of 60 reads. This performance of the Dynabeads Streptavidin for Target Enrichment is particularly important for clinical applications and identification of rare variants where deep coverage (e.g., 60x) is essential.</small></p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#8d83cd5c5d7c41c6bf4bd08917238dd9').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#8d83cd5c5d7c41c6bf4bd08917238dd9').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771" href="#collapse2"><span class="caret"></span><strong> mRNA </strong></a></div><div id="collapse2" class="accordion-body collapse "><div class="accordion-inner container-5d6811503987712"><div class="container-5d6811503987712 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2><br>Streptavidin beads for mRNA synthesis </h2></div><div class="text parbase section"><p>Streptavidin beads can be used for mRNA synthesis by <i>in vitro</i> transcription (IVT), an application/workflow that can greatly accelerate the research and development of mRNA vaccines. The mRNA is synthesized using a biotinylated DNA template of choice, amplified via PCR, and immobilized to streptavidin beads (<b>Figure 6</b>). The immobilized template can be reused in the next IVT reaction at least six times.</p></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="text parbase section"><p><small><b>Figure 6. mRNA synthesis workflow with Dynabeads Streptavidin for <i>In Vitro</i> Transcription.</b> This workflow begins with the biotinylated PCR product. This mRNA template is then immobilized to the <a href="?open=mrna#mrna">Dynabeads Streptavidin for <i>In Vitro</i> Transcription</a> beads forming a bead-template complex. Using this complex, IVT can be performed up to six consecutive times. The target mRNA is then ready to be eluted from the streptavidin beads via magnetic separation and incubation with elution buffer.</small></p></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987712tabimage_8da2"><a class="image-enlarge-link" data-lightbox="image_8da2" href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/_jcr_content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_8da2/backgroundimg.img.jpg/1730136165530.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_8da2/foregroundimg.img.320.high.jpg/1730136165473.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_8da2/foregroundimg.img.480.high.jpg/1730136165473.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_8da2/foregroundimg.img.full.high.jpg/1730136165473.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_8da2/foregroundimg.img.320.high.jpg/1730136165473.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_8da2/foregroundimg.img.480.high.jpg/1730136165473.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_8da2/foregroundimg.img.320.medium.jpg/1730136165473.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_8da2/foregroundimg.img.620.high.jpg/1730136165473.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_8da2/foregroundimg.img.320.low.jpg/1730136165473.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_8da2/foregroundimg.img.480.medium.jpg/1730136165473.jpg" alt='Schematic showing the mRNA synthesis workflow starting with the biotinylated PCR product, then immobilization of target mRNA to streptavidin beads, then in vitro transcription when mRNA target is enriched' title='mRNA synthesis workflow using streptavidin magnetic beads'></picture><style> #cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987712tabimage_8da2 img{ width:100%; max-width: 600px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987712tabimage_8da2 .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987712tabimage_8da2 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div></div><div style="clear:both"></div><div class="heading section"><h3> mRNA synthesis by <i>in vitro</i> transcription (IVT) </h3></div><div class="text parbase section"><p>mRNA synthesis by IVT begins with the biotinylated template, which is made by PCR amplification of the target sequence in a plasmid construct or in a synthetic DNA construct using a biotinylated forward primer located 30–100 bps upstream of the T7-promoter and a non-biotinylated reverse primer (<b>Figure 7</b>). The biotinylated template is immobilized to the streptavidin beads without the need for prior purification. The streptavidin bead-bound template is used directly in IVT, then removed from the synthesized mRNA by magnetic separation. The isolated and purified target regions are subsequently amplified and prepared for sequencing.</p><p><b>Dynabeads Streptavidin for <i>In Vitro</i> Transcription</b> is designed for simple and scalable mRNA synthesis. Product benefits include:</p><ul><li><b>Reusability</b>—reuse the DNA template at least six times to minimize plasmid preparation and reduce hands-on time</li><li><b>Flexibility</b>—choose synthetic or plasmid DNA template for mRNA IVT for greater flexibility of mRNA design</li><li><b>Automation</b>— enables high-throughput synthesis and purification of many samples simultaneously</li></ul></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="text parbase section"><p><small><b>Figure 7. mRNA synthesis by IVT using Dynabeads Streptavidin for <i>In Vitro</i> Transcription.</b> The biotinylated DNA template is immobilized to the streptavidin magnetic beads and contains an upstream linker region, T7 promoter to facilitate binding of the T7 RNA polymerase, and an mRNA coding region. mRNA synthesis can be done up to six times using the same DNA template.</small></p></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987712tabimage_3627"><a class="image-enlarge-link" data-lightbox="image_3627" href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/_jcr_content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/backgroundimg.img.jpg/1730136165741.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.320.low.jpg/1730136165672.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.320.medium.jpg/1730136165672.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.480.medium.jpg/1730136165672.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.620.high.jpg/1730136165672.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.full.high.jpg/1730136165672.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.320.high.jpg/1730136165672.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.480.high.jpg/1730136165672.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.320.medium.jpg/1730136165672.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.620.high.jpg/1730136165672.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.320.low.jpg/1730136165672.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987712/tab/image_3627/foregroundimg.img.480.medium.jpg/1730136165672.jpg" alt='Diagram of how mRNA synthesis with Dynabeads Streptavidin for In Vitro Transcription works' title='mRNA synthesis by IVT using Dynabeads Streptavidin for In Vitro Transcription'></picture><style> #cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987712tabimage_3627 img{ width:100%; max-width: 949px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987712tabimage_3627 .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987712tabimage_3627 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><p><a class="cta-link" href="/sg/en/home/industrial/pharma-biopharma/nucleic-acid-therapeutic-development-solutions/mrna-therapeutic-resources/mrna-synthesis-reagents/magnetic-beads-mrna-production.html">Read more about magnetic bead-based solutions for mRNA synthesis</a><br><a class="cta-link" href="?open=mrna#mrna">Order Dynabeads Streptavidin for <i>In Vitro</i> Transcription now</a></p></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771" href="#collapse3"><span class="caret"></span><strong> Protein and cells </strong></a></div><div id="collapse3" class="accordion-body collapse "><div class="accordion-inner container-5d6811503987713"><div class="container-5d6811503987713 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2><br>Streptavidin beads for protein and cell capture </h2></div><div class="text parbase section"><p>When used in combination with a biotinylated probe/ligand, any target molecule can be captured on the Dynabeads streptavidin magnetic beads, isolated and then further manipulated. Depending on your target molecule and downstream assay, a variety of products are available for protein and cell capture using streptavidin magnetic beads. View <b>Table 2</b> below to determine which product meets your specific research needs.<br>  </p></div><div class="heading section"><h3> Table 2. Streptavidin magnetic beads for direct and indirect isolation </h3></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-937826318"><tbody><tr><th style="width: 15%;">Recommended products</th><th style="width: 15%;"><a></a><a href="/order/catalog/product/11205D">Dynabeads M-280 Streptavidin</a></th><th style="width: 15%;"><a></a><a href="/order/catalog/product/65601">Dynabeads MyOne Streptavidin T1</a></th><th style="width: 15%;"><a></a><a href="/order/catalog/product/11047">Dynabeads Biotin Binder</a></th><th style="width: 15%;"><a></a><a href="/order/catalog/product/11533D">CELLection Biotin Binder Kit</a></th><th style="width: 15%;"><a></a><a href="/order/catalog/product/11061D">Dynabeads FlowComp Flexi Kit</a></th></tr><tr><td><b>Description</b></td><td><ul><li>Hydrophobic bead surface</li><li>Based on tosylactivated beads</li><li>Diameter: 2.8 µm</li><li>Size distribution:<br> CV < 3%</li><li>BSA as blocking protein</li><li>Isoelectric point: pH 5.0</li><li>Low charge (–10 mV (at pH 7)</li><li>Iron content (Ferrites): 12% (17%)</li></ul></td><td><ul><li>Hydrophobic bead surface</li><li>Based on tosylactivated beads</li><li>Diameter: 1.05 µm</li><li>Size distribution: CV <3%</li><li>BSA as blocking protein</li><li>Isoelectric point: pH 5.0</li><li>Low charge (–10 mV (at pH 7)</li><li>Iron content (Ferrites): 26% (37%)</li><li>Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads</li></ul></td><td><ul><li>For use with your own standard biotinylated antibodies</li><li>Isolate cells from whole blood, buffy coat, PBMC, MNC, or tissue digests</li></ul></td><td><ul><li>For use with your own standard biotinylated antibodies</li><li>Streptavidin on the bead-surface is attached via a DNA linker, providing a cleavable site to release and remove the beads after isolation</li></ul></td><td><ul><li>For use with your own standard biotinylated antibodies</li><li>DSB-X biotinylation of the antibody is required to assure release of the target cells from the bead</li></ul></td></tr><tr><td><b>Capacity</b></td><td><ul><li>Free biotin:<br> 490–750<br> pmol/mg beads</li><li>Biotinylated<br> Ig:<br> 5–10 µg/mg<br> beads</li></ul></td><td><ul><li>Free biotin:<br> 950–1,500 pmol/mg beads</li><li>Biotinylated Ig:<br> 20 µg/mg beads</li></ul></td><td>Processes 2 x 10<sup>9</sup> PBMCs or 200 mL whole blood</td><td>Processes 2 x 10<sup>9</sup> PBMCs or 200 mL whole blood</td><td>Processes 2 x 10<sup>9</sup> MNCs or 80 mL whole blood/buffy coat</td></tr><tr><td><b>Ideal applications</b></td><td><ul><li>Immunoassays/<br> Immunodiagnostics (for research use only)</li><li>Protein purification</li><li>Phage display</li><li>Biopanning</li><li>Cell isolation</li></ul></td><td><ul><li>Immunoassays/<br> Immunodiagnostics (for research use only)</li><li>Protein purification</li><li>Phage display</li><li>Biopanning</li><li>Cell isolation</li><li>Automation</li></ul></td><td><ul><li>Depletion of one/multiple cell types from any sample from any species</li><li>Positive cell isolation for molecular downstream applications (without cell release)</li></ul></td><td><ul><li>Positive cell isolation and release</li><li>Isolate any cell from any sample from any species</li></ul></td><td><ul><li>Positive cell isolation and release</li><li>Isolate any cell from any sample from any species</li></ul></td></tr></tbody></table></div></div><div class="heading section"><h3><br>Direct and indirect capture of molecules and cells with streptavidin beads </h3></div><div class="parsys_column cq-colctrl-lt8"><div class="parsys_column cq-colctrl-lt8-c0"><div class="image parbase section"><div id="cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987713tabimage_fdac"><a class="image-enlarge-link" data-lightbox="image_fdac" href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/_jcr_content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987713/tab/image_fdac/backgroundimg.img.gif/1730136166149.gif"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987713/tab/image_fdac/foregroundimg.img.full.high.gif/1730136166079.gif' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987713/tab/image_fdac/foregroundimg.img.full.high.gif/1730136166079.gif,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987713/tab/image_fdac/foregroundimg.img.320.high.gif/1730136166079.gif,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987713/tab/image_fdac/foregroundimg.img.480.high.gif/1730136166079.gif,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987713/tab/image_fdac/foregroundimg.img.320.medium.gif/1730136166079.gif,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987713/tab/image_fdac/foregroundimg.img.620.high.gif/1730136166079.gif,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987713/tab/image_fdac/foregroundimg.img.320.low.gif/1730136166079.gif,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/tabworkflowcontainer_1150398771/container-5d6811503987713/tab/image_fdac/foregroundimg.img.480.medium.gif/1730136166079.gif" alt='Diagram detailing direct capture versus indirect capture of molecules of cells using streptavidin magnetic beads; direct capture uses a pre-coupled ligand that is added to the sample, incubated, then eluted via magnetic separation and indirect capture begins with incubating the biotinylated probe/ligand to the sample then adding the streptavidin magnetic beads, then eluting via magnetic separation' title='Streptavidin magnetic beads for direct and indirect capture of molecules and cells'></picture><style> #cq-image-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987713tabimage_fdac img{ width:100%; max-width: 275px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987713tabimage_fdac .image-contain" + "," + "#cq-image-jsp-"+"792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystabworkflowcontainer_1150398771container-5d6811503987713tabimage_fdac .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div class="text parbase section"><p>Depending on your target molecule and the specific application, the direct or indirect capture method can be applied using streptavidin magnetic beads (<b>Figure 8</b>).</p><p>For some applications, using a pre-coupled ligand in direct capture allows you to reuse the magnetic beads and thus further reduce sample preparation costs.</p><p>The indirect capture approach can be of benefit when the concentration of your target is low, the specific affinity is weak, or the binding kinetics is slow. In indirect capture, the probe/ligand is allowed to bind to the target in suspension prior to addition of the beads. A monolayer of streptavidin is covalently coupled to the magnetic beads, helping to reduce leakage that could otherwise disturb your assay.</p><p><small><b>Figure 8. Direct and indirect capture of molecules and cells with streptavidin magnetic beads.</b> Direct capture uses a pre-coupled ligand that is added to the sample, incubated, then eluted via magnetic separation. Indirect capture begins with incubation of the biotinylated probe/ligand to the sample, then the streptavidin magnetic beads are added and incubated for some time, followed by elution off the magnetic bead by magnetic separation.</small></p><p><a class="cta-link" href="?open=capture#capture">Order Dynabeads streptavidin beads for protein and cell capture now</a></p></div></div></div><div style="clear:both"></div></div><div class="end"></div></div></div></div></div></div></div><div class="heading section"><h2 id="faqs"> Dynabeads streptavidin bead FAQs </h2></div><div class="text parbase section"><p>Here are some frequently asked questions regarding the use of Dynabeads streptavidin magnetic beads.</p></div><div class="accordion section"><div class="accordion" id="8723837151aa493d895d771a94487483" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#8723837151aa493d895d771a94487483" href="#elute"><span class="caret"></span> How do you elute DNA from streptavidin beads? </a></div><div class="accordion-body collapse " id="elute"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="heading section"><h3> How do you elute DNA from streptavidin beads? </h3></div><div class="text parbase section"><p><b>A:</b> Unless derivative forms of biotin or modified streptavidin have been adopted for your experiment, requiring a specific form and normally gentle way to dissociate biotin from streptavidin, often very harsh methods are required to dissociate the biotin from streptavidin which will denature the streptavidin. A couple of these methods are discussed below.</p><p><b>Biotinylated nucleic acids:<br></b> To dissociate biotinylated nucleic acids from streptavidin-coupled Dynabeads, incubate the beads in 95% formamide + 10 mM EDTA, pH 8.2 for 5 minutes at 65°C or for 2 minutes at 90°C. Pull the beads to the tube wall with the magnet and remove the supernatant containing the biotinylated nucleic acid from the tube.</p><p><b>Biotinylated proteins:<br></b> For biotinylated proteins, boil the beads in 0.1% SDS or SDS-PHAGE buffer for 3 min.</p></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#8723837151aa493d895d771a94487483" href="#reuse"><span class="caret"></span> Are the Dynabeads streptavidin beads reusable? </a></div><div class="accordion-body collapse " id="reuse"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p><b>A:</b> For most applications involving dissociation of biotinylated molecules from streptavidin, it is not possible to reuse the beads. The streptavidin-biotin bond is one of the strongest biological bonds known, and the conditions necessary to break this bond also destroy the streptavidin. However, if the Dynabeads streptavidin beads have been used in applications such as isolation of DNA binding proteins and release of proteins from DNA is done by gentle methods like using high-salt buffers that do not interfere with biotin-streptavidin interaction, the beads with the immobilized probe may be reused.</p></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#8723837151aa493d895d771a94487483" href="#elute2"><span class="caret"></span> How do I elute my target from biotinylated antibody without eluting the antibody off the streptavidin beads? </a></div><div class="accordion-body collapse " id="elute2"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p><b>A: </b>Use mild elution methods like buffers with high salt (>1 M NaOH) or low pH. Low pH elution buffers such as 0.1 M glycine•HCl, pH 2.5–3.0 are effective for most antibody-antigen interactions. Note that boiling in SDS will also elute the antibody.</p></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#8723837151aa493d895d771a94487483').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#8723837151aa493d895d771a94487483').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div class="text parbase section"><p><a class="cta-link" href="/search/results?query=Dynabeads%20streptavidin&persona=DocSupport&refinementAction=true&filter=document.result_type_s:Product%20FAQs">View more FAQs about Dynabeads streptavidin magnetic beads</a></p></div><div class="accordion section"><div class="accordion" id="ec17e069be794c2295cc115eeea52f14" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#ec17e069be794c2295cc115eeea52f14" href="#publications"><span class="caret"></span> Publications using Dynabeads strepavidin beads </a></div><div class="accordion-body collapse " id="publications"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="heading section"><h2> Dynabeads streptavidin bead publications </h2></div><div class="text parbase section"><ul class="the-icons clearfix"><li><span class="icon icon-outbound"></span> <a href="https://pubmed.ncbi.nlm.nih.gov/11474017/" target="_blank">Meng Q., Wong C., Rangachari A., et al. (2001) Automated multiplex assay system for simultaneous detection of hepatitis B virus DNA, hepatitis C virus RNA and human immunodeficiency virus type 1 RNA. J Clin Microbiol 39(8):2937–2945.</a></li><li><span class="icon icon-outbound"></span> <a href="https://pubmed.ncbi.nlm.nih.gov/11356880/" target="_blank">Pollock GS., Vernon E., Forbes ME., et al. (2001) Effects of early visual experience and diurnal rhythms on BDNF mRNA and protein levels in the visual system, hippocampus and cerebellum. J Neurosci 21(11):3923–3931.</a></li><li><span class="icon icon-outbound"></span> <a href="https://pubmed.ncbi.nlm.nih.gov/11238304/" target="_blank">Miyashiro I., Kuo C., Huynh K., et al. (2001) Molecular strategy for detecting metastatic cancers with use of multiple tumor-specific MAGE-A genes. Clin Chem 47(3):505–512.</a></li></ul><p><a class="cta-link" href="/sg/en/home/life-science/cell-analysis/cell-isolation-and-expansion/cell-isolation.html?open=publications#publications">View cell isolation publications using Dynabeads magnetic beads</a><br><a class="cta-link" href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation.html?open=publications#publications">View publications on Dynabeads magnetic beads for immunoprecipitation</a></p></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#ec17e069be794c2295cc115eeea52f14').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#ec17e069be794c2295cc115eeea52f14').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div class="heading section"><h2 id="order"> Ordering information for streptavidin magnetic beads </h2></div><div class="accordion section"><div class="accordion" id="6ca57df3d75547bfbe636e583499931c" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#6ca57df3d75547bfbe636e583499931c" href="#seq"><span class="caret"></span> Dynabeads streptavidin beads for target sequence enrichment </a></div><div class="accordion-body collapse " id="seq"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">65605D, 65606D, 65607D</div><div id='ltajax-8992842722364114163'></div><script type="text/javascript">(function() {$('#ltajax-8992842722364114163').load('/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/accordion_50fc/itemspar/accordionitem_e2ac/itemParsys/productlist_2f2f.ajax.html?skulist=');})(); </script></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#6ca57df3d75547bfbe636e583499931c" href="#mrna"><span class="caret"></span> Dynabeads streptavidin beads for mRNA synthesis </a></div><div class="accordion-body collapse " id="mrna"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">65005D, 65006D</div><div id='ltajax-4770506646107784490'></div><script type="text/javascript">(function() {$('#ltajax-4770506646107784490').load('/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/accordion_50fc/itemspar/accordionitem_b4d2/itemParsys/productlist_abfe.ajax.html?skulist=');})(); </script></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#6ca57df3d75547bfbe636e583499931c" href="#capture"><span class="caret"></span> Dynabeads streptavidin beads for protein and cell capture </a></div><div class="accordion-body collapse " id="capture"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">65605D, 65606D, 65607D, 65305, 65306, 11205D, 11206D, 60210, 65601, 65602, 65604D, 65001, 65002, 65801D</div><div id='ltajax-7864360929795683134'></div><script type="text/javascript">(function() {$('#ltajax-7864360929795683134').load('/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/accordion_50fc/itemspar/accordionitem_5119/itemParsys/productlist_6b14.ajax.html?skulist=');})(); </script></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#6ca57df3d75547bfbe636e583499931c').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#6ca57df3d75547bfbe636e583499931c').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystextimage_b28f"><a href="/sg/en/home/life-science/sequencing/next-generation-sequencing/ion-torrent-next-generation-sequencing-workflow/library-cleanup-pcr-purification.html"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/textimage_b28f/image.img.full.high.jpg/1730136167326.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/textimage_b28f/image.img.full.high.jpg/1730136167326.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/textimage_b28f/image.img.320.high.jpg/1730136167326.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/textimage_b28f/image.img.480.high.jpg/1730136167326.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/textimage_b28f/image.img.320.medium.jpg/1730136167326.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/textimage_b28f/image.img.620.high.jpg/1730136167326.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/textimage_b28f/image.img.320.low.jpg/1730136167326.jpg,https://www.thermofisher.com/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/jcr:content/MainParsys/textimage_b28f/image.img.480.medium.jpg/1730136167326.jpg" alt='Plastic bottle containing brown-colored MagMAX Pure Bind Beads' title='MagMAX Pure Bind Beads'></picture></div><style> #cq-textimage-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystextimage_b28f { max-width: 100%; } #cq-textimage-jsp-792110b5-18fb-4e7c-b1b7-58b636b60171MainParsystextimage_b28f img { width: 100%; max-width: 278px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><h2><br>Magnetic beads for PCR & NGS library prep size selection and cleanup</h2><p>Need size selection and cleanup? Take your library prep to the next level with MagMAX Pure Bind Beads—cost-effective, energy-efficient, and high-performing magnetic beads.</p><h4><a href="/sg/en/home/life-science/sequencing/next-generation-sequencing/ion-torrent-next-generation-sequencing-workflow/library-cleanup-pcr-purification.html" class="cta-link">Learn more</a></h4></div></div><div class="clearfix"></div></div><div class="divider section"><hr /></div><div class="well-cmp section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><div class="h2" id="resources"> Streptavidin beads related content </div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><ul class="the-icons"><li><a href="/sg/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/avidin-biotin-interaction.html">Avidin-Biotin Interaction</a></li><li><a href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/capture-of-biotinylated-targets.html">Guidelines for Biotinylation</a></li><li><a href="/sg/en/home/life-science/cell-analysis/cell-isolation-and-expansion/cell-isolation/cell-isolation-using-biotinylated-antibodies.html">Cell Isolation using Biotinylated Antibodies</a></li><li><a href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/gene-expression-analysis.html">Genome/Gene expression analysis</a></li><li><a href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/subtractive-hybridization.html">Subtractive Hybridization </a></li><li><a href="/sg/en/home/brands/product-brand/dynal/magnets.html">Magnetic Racks and Accessories</a></li><li><a href="/sg/en/home/products-and-services/promotions/dynabeads-oem-plus.html">Dynabeads OEM Technology</a></li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><h3>Literature</h3><ul class="the-icons"><li><span class="icon-lt-pdf"></span> <a href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/mutation-detection-sensitivity-ffpe-tissue-liquid-biopsy-samples-app-note.pdf" target="_blank">Application Note: Mutation detection sensitivity in matched FFPE tissue and liquid biopsy samples</a></li><li><span class="icon-lt-pdf"></span> <a href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/highly-efficient-depletion-rrna-using-ribominus-bacteria-2-kit-app-note.pdf" target="_blank">Application Note: Highly efficient depletion of rRNA using the RiboMinus Bacteria 2.0 kit</a></li><li><span class="icon-pdf"></span> <a class="headline" href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/dynabeads-streptavidin-manual-automated-protocols-brochure.pdf" target="_blank">Brochure: Dynabeads streptavidin products for manual and automated protocols</a></li><li><span class="icon-lt-pdf"></span> <a href="https://assets.thermofisher.com/TFS-Assets/LSG/brochures/OEM_Capabilities.pdf" target="_blank">Brochure: Dynabeads OEM capabilities</a></li><li><span class="icon-lt-pdf"></span> <a href="https://assets.thermofisher.com/TFS-Assets/BID/posters/bead-based-target-enrichment-next-gen-sequencing-readouts-poster.pdf" target="_blank">Poster: Bead-based target enrichment generates high-quality library preparation for enhanced next-generation sequencing readouts</a></li></ul><p></p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="well-cmp"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><div class="h2"> Resources </div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><ul class="the-icons clearfix"><li><a href="/sg/en/home/brands/product-brand/dynal.html">Dynabeads Magnetic Bead Separation Technology</a></li><li><a href="/sg/en/home/life-science/dna-rna-purification-analysis/learning-center.html">DNA & RNA Learning Center</a></li><li><a href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation.html">Immunoprecipitation with Magnetic Beads</a></li><li><a href="/sg/en/home/life-science/cell-analysis/cell-isolation-and-expansion/cell-isolation.html">Cell Isolation</a></li><li><a href="/sg/en/home/life-science/cell-analysis/cell-isolation-and-expansion/cell-expansion.html">T Cell Activation & Expansion</a></li><li><a href="/sg/en/home/life-science/dna-rna-purification-analysis.html">Nucleic Acid Isolation</a></li><li><a href="/sg/en/home/life-science/cell-analysis/exosomes.html">Exosome Isolation, Detection, and Analysis</a></li><li><a href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads.html">Streptavidin-Coupled Dynabeads</a></li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><ul class="the-icons clearfix"><li><a href="/sg/en/home/products-and-services/promotions/dynabeads-oem-plus.html">IVD Assay Development</a></li><li><a href="/sg/en/home/clinical/diagnostic-development/molecular-diagnostic-test-development/bead-based-ivd-assays/iso-certification.html">ISO Certification</a></li><li><a href="/sg/en/home/clinical/diagnostic-development/molecular-diagnostic-test-development/bead-based-ivd-assays/customized-dynabeads-oem-supply.html">Customized Beads for OEM</a></li><li><a href="/sg/en/home/clinical/diagnostic-development/molecular-diagnostic-test-development/bead-based-ivd-assays/intellectual-property.html">Intellectual Property</a></li><li><a href="/sg/en/home/technical-resources/contact-us.html">Technical Support</a></li></ul><ul class="the-icons clearfix"><li><span class="icon-lt-pdf"></span> <a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/dynabeads-magnetic-beads-brochure.pdf" target="_blank">Brochure: Dynabeads magnetic beads</a></li><li><span class="icon-lt-pdf"></span> <a href="https://assets.thermofisher.com/TFS-Assets/LSG/brochures/OEM_Capabilities.pdf" target="_blank">Flyer: OEM Capabilities</a></li></ul></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="ltrawhtml_d1d9 LTRawHTML"><style> /* Remove horizontal scroll on hero banner table */ .wellPageHeading .well-container .table-component-wrapper table 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