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FocalPlane - Where biology meets microscopy
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It is a place where you can interact with a global community of imaging scientists, engineers, chemists, and bioimage analysts. </p> <p>FocalPlane is your site and once registered, you are free to share a blog post, add an image to our <a href="/alt-gallery/">gallery</a>, post a <a href="/category/jobs/">job advert </a>or put up an <a href="/events/">event listing</a>.</p> <p>Create an account to share your story or to stay up-to-date with the latest news.</p> <div class="wp-block-buttons"> <div class="wp-block-button"><a class="wp-block-button__link wp-element-button" href="https://focalplane.biologists.com/wp-login.php?action=register">Register here</a></div> </div> <p></p> </div> <p></p> </div> <div class="wp-block-column" style="flex-basis:33.33%"> <div style="height:20px" aria-hidden="true" class="wp-block-spacer"></div> <figure class="wp-block-image size-full"><a href="https://focalplane.biologists.com/network/"><img fetchpriority="high" decoding="async" width="1024" height="768" data-attachment-id="15923" data-permalink="https://focalplane.biologists.com/focalplane-network-slide-standard/" data-orig-file="https://focalplane.biologists.com/wp-content/uploads/2023/08/FocalPlane-Network-Slide-Standard.jpg" data-orig-size="1024,768" data-comments-opened="1" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="FocalPlane Network Slide Standard" data-image-description="" data-image-caption="" data-medium-file="https://focalplane.biologists.com/wp-content/uploads/2023/08/FocalPlane-Network-Slide-Standard-300x225.jpg" data-large-file="https://focalplane.biologists.com/wp-content/uploads/2023/08/FocalPlane-Network-Slide-Standard.jpg" src="https://focalplane.biologists.com/wp-content/uploads/2023/08/FocalPlane-Network-Slide-Standard.jpg" alt="" class="wp-image-15923" srcset="https://focalplane.biologists.com/wp-content/uploads/2023/08/FocalPlane-Network-Slide-Standard.jpg 1024w, https://focalplane.biologists.com/wp-content/uploads/2023/08/FocalPlane-Network-Slide-Standard-300x225.jpg 300w, https://focalplane.biologists.com/wp-content/uploads/2023/08/FocalPlane-Network-Slide-Standard-150x113.jpg 150w, https://focalplane.biologists.com/wp-content/uploads/2023/08/FocalPlane-Network-Slide-Standard-768x576.jpg 768w" sizes="(max-width: 1024px) 100vw, 1024px" /></a></figure> <div class="wp-block-buttons"> <div class="wp-block-button"><a class="wp-block-button__link wp-element-button" href="https://focalplane.biologists.com/network/">Join or access here</a></div> </div> </div> </div> <div class="wp-block-columns"> <div class="wp-block-column" style="flex-basis:66.66%"> <h2 class="wp-block-heading">Recent posts</h2> <div class="wp-block-group is-style-group--white-watermark"> <div class="wp-block-group__inner-container"> <h3><a href="https://focalplane.biologists.com/2025/03/29/sted-microscopy-user-group-april-10-12pm-edt/">STED Microscopy User Group, April 10, 12pm EDT</a></h3> <p>Posted by <span><a href="https://focalplane.biologists.com/author/eap2143/" title="Posts by Ethan A. Perets" rel="author">Ethan A. Perets</a></span>, on 29 March 2025</p> The next BioImaging North America and Canada BioImaging STED user group will take place via Zoom on April 10 at 12 pm EST. Mathias Delhaye and Jeffrey LeDue will jointly deliver the presentation, “Expanding Insights: Magnified Analysis of the Proteome Across Imaging Platforms”, followed by Q&A. We hope you will join us! This user group is targeted at <div class="wp-block-button"><a class="wp-block-button__link" href="https://focalplane.biologists.com/2025/03/29/sted-microscopy-user-group-april-10-12pm-edt/">Read more</a></div> </div> </div> <div class="wp-block-group is-style-group--grey-watermark"> <div class="wp-block-group__inner-container"> <h3><a href="https://focalplane.biologists.com/2025/03/27/announcing-the-winners-of-the-node-focalplane-image-competition/">Announcing the winners of the Node–FocalPlane image competition.</a></h3> <p>Posted by <span><a href="https://focalplane.biologists.com/author/focalplane/" title="Posts by FocalPlane" rel="author">FocalPlane</a></span>, on 27 March 2025</p> <div class="wp-block-image"><figure class="alignleft size-large is-resized"><img decoding="async" src="https://focalplane.biologists.com/wp-content/uploads/2025/03/15.-Cell-estial-Bloom-1024x1024.jpg" alt="" width="120"></figure></div> We are delighted to announce the winners of the Node–FocalPlane image competition, which we presented at the Biologists @ 100 conference, as well as on the Node and FocalPlane. In first place is ‘Cell-estial bloom’ from Özge Özgüç. Özge’s image is a colony of live hiPSCs, with fluorescently labelled Lamin B delineating the nuclear lamina <div class="wp-block-button"><a class="wp-block-button__link" href="https://focalplane.biologists.com/2025/03/27/announcing-the-winners-of-the-node-focalplane-image-competition/">Read more</a></div> </div> </div> <div class="wp-block-group is-style-group--white-watermark"> <div class="wp-block-group__inner-container"> <h3><a href="https://focalplane.biologists.com/2025/03/23/illuminating-cancer-metabolism-unveiling-mitochondrial-secrets-through-fluorescence-microscopy/">Illuminating Cancer Metabolism: Unveiling Mitochondrial Secrets Through Fluorescence Microscopy</a></h3> <p>Posted by <span><a href="https://focalplane.biologists.com/author/polleysangramgmail-com/" title="Posts by SANGRAM POLLEY" rel="author">SANGRAM POLLEY</a></span>, on 23 March 2025</p> <div class="wp-block-image"><figure class="alignleft size-large is-resized"><img decoding="async" src="https://focalplane.biologists.com/wp-content/uploads/2025/03/Screenshot-2025-03-23-220537.png" alt="" width="120"></figure></div> Understanding cancer metabolism is central to developing effective therapies, and a chief player in this complex scenario is mitochondrial metabolism. The studies of Monika Gooz and Eduardo N. Maldonado have shed light on how fluorescence microscopy is employed to investigate mitochondrial activity in cancer cells, thus posing avenues into the metabolic vulnerabilities that could be <div class="wp-block-button"><a class="wp-block-button__link" href="https://focalplane.biologists.com/2025/03/23/illuminating-cancer-metabolism-unveiling-mitochondrial-secrets-through-fluorescence-microscopy/">Read more</a></div> </div> </div> <div class="wp-block-group is-style-group--grey-watermark"> <div class="wp-block-group__inner-container"> <h3><a href="https://focalplane.biologists.com/2025/03/21/microscopy-preprints-new-tools-and-techniques-in-imaging-15/">Microscopy preprints – new tools and techniques in imaging</a></h3> <p>Posted by <span><a href="https://focalplane.biologists.com/author/focalplane/" title="Posts by FocalPlane" rel="author">FocalPlane</a></span>, on 21 March 2025</p> <div class="wp-block-image"><figure class="alignleft size-large is-resized"><img decoding="async" src="https://focalplane.biologists.com/wp-content/uploads/2025/03/preprints210325-150x135.png" alt="" width="120"></figure></div> Here is a curated selection of preprints posted recently on new tools and techniques in imaging. Let us know if we are missing any recent preprints that are on your reading list! <div class="wp-block-button"><a class="wp-block-button__link" href="https://focalplane.biologists.com/2025/03/21/microscopy-preprints-new-tools-and-techniques-in-imaging-15/">Read more</a></div> </div> </div> <div class="wp-block-group is-style-group--white-watermark"> <div class="wp-block-group__inner-container"> <h3><a href="https://focalplane.biologists.com/2025/03/17/biologists-100-video-guide/">Biologists @ 100 video guide</a></h3> <p>Posted by <span><a href="https://focalplane.biologists.com/author/focalplane/" title="Posts by FocalPlane" rel="author">FocalPlane</a></span>, on 17 March 2025</p> <div class="wp-block-image"><figure class="alignleft size-large is-resized"><img decoding="async" src="https://focalplane.biologists.com/wp-content/uploads/2025/03/Screenshot-2025-03-17-111132-150x84.png" alt="" width="120"></figure></div> It’s only one week to go until the start of the Biologists @ 100 conference and we’re looking forward to welcoming you in Liverpool. Together with our sister community sites, the Node and preLights, we’ve made a recording to give you a flavour of what to expect during the conference. <div class="wp-block-button"><a class="wp-block-button__link" href="https://focalplane.biologists.com/2025/03/17/biologists-100-video-guide/">Read more</a></div> </div> </div> <div class="wp-block-group is-style-group--grey-watermark"> <div class="wp-block-group__inner-container"> <h3><a href="https://focalplane.biologists.com/2025/03/14/featured-image-with-omkar-joshi/">Featured image with Omkar Joshi</a></h3> <p>Posted by <span><a href="https://focalplane.biologists.com/author/focalplane/" title="Posts by FocalPlane" rel="author">FocalPlane</a></span>, on 14 March 2025</p> <div class="wp-block-image"><figure class="alignleft size-large is-resized"><img decoding="async" src="https://focalplane.biologists.com/wp-content/uploads/2025/03/Screenshot-2025-03-03-120150-1020x1024.png" alt="" width="120"></figure></div> Our featured image, acquired by Omkar Joshi, showcases a U2OS cell immunostained for paxillin (green), TOMM20 (mitochondria, white), and F-actin (labelled using SiR-Actin, magenta), with DAPI (yellow) marking the nucleus. <div class="wp-block-button"><a class="wp-block-button__link" href="https://focalplane.biologists.com/2025/03/14/featured-image-with-omkar-joshi/">Read more</a></div> </div> </div> <div class="wp-block-group is-style-group--white-watermark"> <div class="wp-block-group__inner-container"> <h3><a href="https://focalplane.biologists.com/2025/03/11/vote-for-your-favourite-image/">Vote for your favourite image</a></h3> <p>Posted by <span><a href="https://focalplane.biologists.com/author/focalplane/" title="Posts by FocalPlane" rel="author">FocalPlane</a></span>, on 11 March 2025</p> <div class="wp-block-image"><figure class="alignleft size-large is-resized"><img decoding="async" src="https://focalplane.biologists.com/wp-content/uploads/2025/03/Imagecomp_1-150x85.png" alt="" width="120"></figure></div> We are delighted to unveil the shortlisted images in the Node–FocalPlane image competition. Thanks to everyone that entered, we received so many fantastic images and congratulations to all the finalists. You can vote for your favourite image using the poll at the bottom of the page. <div class="wp-block-button"><a class="wp-block-button__link" href="https://focalplane.biologists.com/2025/03/11/vote-for-your-favourite-image/">Read more</a></div> </div> </div> <div class="wp-block-group is-style-group--clear"> <div class="wp-block-group__inner-container"> <div class="wp-block-button alignleft"><a class="wp-block-button__link" href="/?s=">View more posts …</a></div> </div> </div> </div> <div class="wp-block-column" style="flex-basis:33.33%"> <div style="height:20px" aria-hidden="true" class="wp-block-spacer"></div> <figure class="wp-block-image size-full is-resized"><a href="https://www.biologists.com/grants/jcs-focalplane-training-grants/"><img decoding="async" width="300" height="250" data-attachment-id="20816" data-permalink="https://focalplane.biologists.com/homepage/jcs-fp-training-300x250banner/" data-orig-file="https://focalplane.biologists.com/wp-content/uploads/2024/07/JCS-FP-Training-300x250Banner.jpg" data-orig-size="300,250" data-comments-opened="1" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="JCS-FP-Training-300x250Banner" data-image-description="" data-image-caption="" data-medium-file="https://focalplane.biologists.com/wp-content/uploads/2024/07/JCS-FP-Training-300x250Banner.jpg" data-large-file="https://focalplane.biologists.com/wp-content/uploads/2024/07/JCS-FP-Training-300x250Banner.jpg" src="https://focalplane.biologists.com/wp-content/uploads/2024/07/JCS-FP-Training-300x250Banner.jpg" alt="" class="wp-image-20816" style="width:567px;height:auto" srcset="https://focalplane.biologists.com/wp-content/uploads/2024/07/JCS-FP-Training-300x250Banner.jpg 300w, https://focalplane.biologists.com/wp-content/uploads/2024/07/JCS-FP-Training-300x250Banner-150x125.jpg 150w" sizes="(max-width: 300px) 100vw, 300px" /></a></figure> <div class="wp-block-group is-style-group--white"> <h2 class="wp-block-heading">Most read on FocalPlane</h2> <ul class="wp-block-list"> <li><a href="https://focalplane.biologists.com/resources/">MicroList resources</a></li> <li><a href="https://focalplane.biologists.com/2024/09/06/microscopy-preprints-bioimage-analysis-5/">Microscopy preprints – bioimage analysis</a></li> <li><a href="https://focalplane.biologists.com/gallery/">Gallery</a></li> <li><a href="https://focalplane.biologists.com/2024/09/13/featured-image-with-ciaran-butler-hallissey/">Featured image with Ciarán Butler Hallissey</a></li> </ul> </div> <div class="wp-block-group is-style-group--orange"><div class="wp-block-group__inner-container"> <h2 class="wp-block-heading">Questions about FocalPlane?</h2> <div class="wp-block-buttons"> <div class="wp-block-button"><a class="wp-block-button__link" href="https://focalplane.biologists.com/frequently-asked-questions/">FAQs</a></div> </div> </div></div> <div class="wp-block-group is-style-group--orange"><div class="wp-block-group__inner-container"> <h2 class="wp-block-heading">Become a sponsor</h2> <p>Are you interested in becoming a sponsor for FocalPlane?</p> <div class="wp-block-button alignleft"><a class="wp-block-button__link" href="/contact-us/">Contact the team</a></div> </div></div> <div class="wp-block-group is-style-group--white"> <h2 class="wp-block-heading">Our sister sites</h2> <div class="wp-block-group"> <figure class="wp-block-image size-large is-resized"><a href="https://thenode.biologists.com"><img decoding="async" src="https://www.biologists.com/wp-content/uploads/2015/07/TheNode_RGB_AW-250x127.png" alt="the Node" width="125" height="64"/></a></figure> <p class="has-normal-font-size">The community site for <br>developmental biologists</p> <div class="wp-block-buttons"> <div class="wp-block-button"><a class="wp-block-button__link wp-element-button" href="https://thenode.biologists.com/">Join in</a></div> </div> </div> <div class="wp-block-group"> <figure class="wp-block-image alignleft size-medium is-resized"><a href="https://prelights.biologists.com/"><img loading="lazy" decoding="async" data-attachment-id="1368" data-permalink="https://focalplane.biologists.com/prelights_logo_rgb/" data-orig-file="https://focalplane.biologists.com/wp-content/uploads/2020/05/preLights_logo_RGB-e1593078621852.png" data-orig-size="250,87" data-comments-opened="1" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="preLights_logo_RGB" data-image-description="" data-image-caption="" data-medium-file="https://focalplane.biologists.com/wp-content/uploads/2020/05/preLights_logo_RGB-300x105.png" data-large-file="https://focalplane.biologists.com/wp-content/uploads/2020/05/preLights_logo_RGB-1024x357.png" src="https://focalplane.biologists.com/wp-content/uploads/2020/05/preLights_logo_RGB-300x105.png" alt="the Node" class="wp-image-1368" width="225" height="79"/></a></figure> <div class="wp-block-buttons"> <div class="wp-block-button"><a class="wp-block-button__link wp-element-button" href="https://prelights.biologists.com/">Join in</a></div> </div> </div> </div> <div class="wp-block-group"> <h2 class="wp-block-heading">Image credits</h2> <p><a href="https://focalplane.biologists.com/2023/09/01/and-the-winner-is/">Neuromuscular junctions</a> – Rebecca Simkins</p> <p><a href="https://focalplane.biologists.com/2023/11/14/featured-image-with-till-stephan/">Mitochondria and microtubules</a> – Till Stephan</p> <p><a href="https://focalplane.biologists.com/2023/11/15/featured-image-with-oona-paavolainen/">Mammary gland organoid</a> – Oona Paavolainen</p> <p><a href="https://focalplane.biologists.com/2023/11/16/featured-image-with-nick-gatford/">Dopaminergic neuron</a> – Nick Gatford</p> <p>CLEM HeLa cell – Marie-Charlotte Domart, Chris Peddie</p> </div> <p></p> </div> </div> <div class="wp-block-columns"> <div class="wp-block-column" style="flex-basis:66.66%"> <div class="wp-block-group is-style-group--clear"><h2>Posts by categories</h2><div class='block-category-list--as-images col-md-12 images-per-row-3'><div class="col-xs-4 col-md-4 "><a href="/category/discussions"><img decoding="async" class="opacity-10" src="https://focalplane.biologists.com/wp-content/uploads/2020/04/discussions-with-title.png" alt="Discussions" /></a></div><div class="col-xs-4 col-md-4 "><a href="/category/how-to"><img decoding="async" class="opacity-6" src="https://focalplane.biologists.com/wp-content/uploads/2020/04/how-to-with-title.png" alt="How to" /></a></div><div class="col-xs-4 col-md-4 "><a href="/category/tools"><img decoding="async" class="opacity-10" src="https://focalplane.biologists.com/wp-content/uploads/2020/04/tools-with-title.png" alt="Tools" /></a></div><div class="col-xs-4 col-md-4 "><a href="/category/case-studies"><img decoding="async" class="opacity-5" src="https://focalplane.biologists.com/wp-content/uploads/2020/04/case-studies-with-title.png" alt="Case studies" /></a></div><div class="col-xs-4 col-md-4 "><a href="/category/interviews"><img decoding="async" class="opacity-99" src="https://focalplane.biologists.com/wp-content/uploads/2020/04/interviews-with-title.png" alt="Interviews" /></a></div><div class="col-xs-4 col-md-4 "><a href="/events/"><img decoding="async" class="opacity-10" src="/wp-content/uploads/2020/04/events-with-title.png" alt="Events" /></a></div><div class="col-xs-4 col-md-4 "><a href="https://focalplane.biologists.com/jobs/"><img decoding="async" class="opacity-6" src="https://focalplane.biologists.com/wp-content/uploads/2020/04/jobs-with-title.png" alt="Jobs" /></a></div><div class="col-xs-4 col-md-4 "><a href="/category/education"><img decoding="async" class="opacity-10" src="https://focalplane.biologists.com/wp-content/uploads/2020/06/education.jpg" alt="Education" /></a></div><div class="col-xs-4 col-md-4 "><a href="https://focalplane.biologists.com/blog-series/"><img decoding="async" class="opacity-7" src="https://focalplane.biologists.com/wp-content/uploads/2020/12/blog-series-3.png" alt="Blog series" /></a></div></div><div class='clear'></div></div> </div> <div class="wp-block-column" style="flex-basis:33.33%"> <div class="wp-block-group"> <h2 class="wp-block-heading">Microscopy-related articles from our journals</h2> <ul class="wp-block-list"> <li><a href="https://journals.biologists.com/dev/article/151/4/dev202307/344014/HCR-spectral-imaging-10-plex-quantitative-high"><strong>HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples</strong></a> Development 2024 151: dev202307</li> <li><a href="https://journals.biologists.com/jcs/article/137/4/jcs261274/343148/filoVision-using-deep-learning-and-tip-markers-to"><strong>filoVision – using deep learning and tip markers to automate filopodia analysis</strong></a> J Cell Sci 2024 137: jcs261274</li> <li><a href="https://journals.biologists.com/bio/article/13/3/bio060267/344042/Computational-tools-for-quantifying-actin-filament"><strong>C</strong></a><a href="https://journals.biologists.com/bio/article/13/3/bio060267/344042/Computational-tools-for-quantifying-actin-filament?searchresult=1"><strong>omputational tools for quantifying actin filament numbers, lengths, and bundling</strong></a><a href="https://journals.biologists.com/dmm/article/16/6/dmm049908/308879/An-automated-microscopy-workflow-to-study-Shigella"><strong><strong> </strong></strong></a>Biology Open 2024 13: bio.060267</li> </ul> </div> <div class="wp-block-group"> <h2 class="wp-block-heading">Microscopy-related preprint highlights</h2> <figure class="wp-block-image size-large is-resized"><img loading="lazy" decoding="async" width="1024" height="357" data-attachment-id="1368" data-permalink="https://focalplane.biologists.com/prelights_logo_rgb/" data-orig-file="https://focalplane.biologists.com/wp-content/uploads/2020/05/preLights_logo_RGB-e1593078621852.png" data-orig-size="250,87" data-comments-opened="1" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="preLights_logo_RGB" data-image-description="" data-image-caption="" data-medium-file="https://focalplane.biologists.com/wp-content/uploads/2020/05/preLights_logo_RGB-300x105.png" data-large-file="https://focalplane.biologists.com/wp-content/uploads/2020/05/preLights_logo_RGB-1024x357.png" src="https://focalplane.biologists.com/wp-content/uploads/2020/05/preLights_logo_RGB-1024x357.png" alt="" class="wp-image-1368" style="width:222px;height:75px"/></figure> <div style="height:20px" aria-hidden="true" class="wp-block-spacer"></div> <ul class="wp-block-list"> <li><a href="https://prelights.biologists.com/highlights/a-window-into-mammalian-basement-membrane-development-insights-from-the-mturq2-col4a1-mouse-model/"><strong>Illuminating Insights into basement membrane dynamics</strong></a></li> <li><a href="https://prelights.biologists.com/highlights/competition-and-synergy-of-arp2-3-and-formins-in-nucleating-actin-waves/"><strong>Actin polymerization, wave after wave</strong></a></li> <li><a href="https://prelights.biologists.com/highlights/excitable-rho-dynamics-drive-cell-contractions-by-sequentially-inducing-erm-protein-mediated-actin-membrane-attachment-and-actomyosin-contractility/"><strong>“Unraveling cellular dance”: Rho directs edge retractions, ERMs lead the rhythm.</strong></a></li> </ul> </div> </div> </div> <div class="wp-block-columns"> <div class="wp-block-column" style="flex-basis:66.66%"> <div class="wp-block-group"> <h2 class="wp-block-heading">From the gallery</h2> <div data-carousel-extra='{"blog_id":1,"permalink":"https:\/\/focalplane.biologists.com\/"}' id='gallery-1' class='gallery galleryid-291 gallery-columns-3 gallery-size-medium'><figure class='gallery-item'> <div class='gallery-icon portrait'> <a href='https://focalplane.biologists.com/2024/11/22/featured-image-with-md-hashim-reza/cover-image/'><img loading="lazy" decoding="async" width="250" height="300" src="https://focalplane.biologists.com/wp-content/uploads/2024/11/Cover-Image-250x300.jpg" class="attachment-medium size-medium" alt="" srcset="https://focalplane.biologists.com/wp-content/uploads/2024/11/Cover-Image-250x300.jpg 250w, https://focalplane.biologists.com/wp-content/uploads/2024/11/Cover-Image-855x1024.jpg 855w, https://focalplane.biologists.com/wp-content/uploads/2024/11/Cover-Image-125x150.jpg 125w, https://focalplane.biologists.com/wp-content/uploads/2024/11/Cover-Image-768x920.jpg 768w, https://focalplane.biologists.com/wp-content/uploads/2024/11/Cover-Image-1282x1536.jpg 1282w, https://focalplane.biologists.com/wp-content/uploads/2024/11/Cover-Image-1710x2048.jpg 1710w" sizes="(max-width: 250px) 100vw, 250px" data-attachment-id="23501" data-permalink="https://focalplane.biologists.com/2024/11/22/featured-image-with-md-hashim-reza/cover-image/" data-orig-file="https://focalplane.biologists.com/wp-content/uploads/2024/11/Cover-Image-scaled.jpg" data-orig-size="2137,2560" data-comments-opened="1" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="Cover Image" data-image-description="" data-image-caption="" data-medium-file="https://focalplane.biologists.com/wp-content/uploads/2024/11/Cover-Image-250x300.jpg" data-large-file="https://focalplane.biologists.com/wp-content/uploads/2024/11/Cover-Image-855x1024.jpg" /></a> </div></figure><figure class='gallery-item'> <div class='gallery-icon portrait'> <a href='https://focalplane.biologists.com/2024/12/20/featured-image-with-the-hughes-lab/joces-2024-262038-cover-candidate/'><img loading="lazy" decoding="async" width="297" height="300" src="https://focalplane.biologists.com/wp-content/uploads/2024/11/JOCES-2024-262038-cover-candidate-297x300.jpg" class="attachment-medium size-medium" alt="" srcset="https://focalplane.biologists.com/wp-content/uploads/2024/11/JOCES-2024-262038-cover-candidate-297x300.jpg 297w, https://focalplane.biologists.com/wp-content/uploads/2024/11/JOCES-2024-262038-cover-candidate-1012x1024.jpg 1012w, https://focalplane.biologists.com/wp-content/uploads/2024/11/JOCES-2024-262038-cover-candidate-148x150.jpg 148w, https://focalplane.biologists.com/wp-content/uploads/2024/11/JOCES-2024-262038-cover-candidate-768x777.jpg 768w, https://focalplane.biologists.com/wp-content/uploads/2024/11/JOCES-2024-262038-cover-candidate-1519x1536.jpg 1519w, https://focalplane.biologists.com/wp-content/uploads/2024/11/JOCES-2024-262038-cover-candidate.jpg 1749w" sizes="(max-width: 297px) 100vw, 297px" data-attachment-id="23495" data-permalink="https://focalplane.biologists.com/2024/12/20/featured-image-with-the-hughes-lab/joces-2024-262038-cover-candidate/" data-orig-file="https://focalplane.biologists.com/wp-content/uploads/2024/11/JOCES-2024-262038-cover-candidate.jpg" data-orig-size="1749,1769" data-comments-opened="1" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="JOCES-2024-262038 cover candidate" data-image-description="" data-image-caption="" data-medium-file="https://focalplane.biologists.com/wp-content/uploads/2024/11/JOCES-2024-262038-cover-candidate-297x300.jpg" data-large-file="https://focalplane.biologists.com/wp-content/uploads/2024/11/JOCES-2024-262038-cover-candidate-1012x1024.jpg" /></a> </div></figure><figure class='gallery-item'> <div class='gallery-icon landscape'> <a href='https://focalplane.biologists.com/2024/12/06/featured-image-with-jiwon-lee/organelles_plasmodium/'><img loading="lazy" decoding="async" width="300" height="219" src="https://focalplane.biologists.com/wp-content/uploads/2024/11/organelles_plasmodium-300x219.jpg" class="attachment-medium size-medium" alt="" srcset="https://focalplane.biologists.com/wp-content/uploads/2024/11/organelles_plasmodium-300x219.jpg 300w, https://focalplane.biologists.com/wp-content/uploads/2024/11/organelles_plasmodium-150x109.jpg 150w, https://focalplane.biologists.com/wp-content/uploads/2024/11/organelles_plasmodium.jpg 648w" sizes="(max-width: 300px) 100vw, 300px" data-attachment-id="23489" data-permalink="https://focalplane.biologists.com/2024/12/06/featured-image-with-jiwon-lee/organelles_plasmodium/" data-orig-file="https://focalplane.biologists.com/wp-content/uploads/2024/11/organelles_plasmodium.jpg" data-orig-size="648,472" data-comments-opened="1" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="organelles_plasmodium" data-image-description="" data-image-caption="" data-medium-file="https://focalplane.biologists.com/wp-content/uploads/2024/11/organelles_plasmodium-300x219.jpg" data-large-file="https://focalplane.biologists.com/wp-content/uploads/2024/11/organelles_plasmodium.jpg" /></a> </div></figure><figure class='gallery-item'> <div class='gallery-icon landscape'> <a href='https://focalplane.biologists.com/imagej1-54funitmicron-4/'><img loading="lazy" decoding="async" width="300" height="300" src="https://focalplane.biologists.com/wp-content/uploads/2024/08/David-Gaboriau_MAX_Image-4_Structured-Illumination_zcrop_MIP_fluo_merge-2.tif-RGB-1-300x300.jpg" class="attachment-medium size-medium" alt="" aria-describedby="gallery-1-21630" srcset="https://focalplane.biologists.com/wp-content/uploads/2024/08/David-Gaboriau_MAX_Image-4_Structured-Illumination_zcrop_MIP_fluo_merge-2.tif-RGB-1-300x300.jpg 300w, https://focalplane.biologists.com/wp-content/uploads/2024/08/David-Gaboriau_MAX_Image-4_Structured-Illumination_zcrop_MIP_fluo_merge-2.tif-RGB-1-1024x1024.jpg 1024w, https://focalplane.biologists.com/wp-content/uploads/2024/08/David-Gaboriau_MAX_Image-4_Structured-Illumination_zcrop_MIP_fluo_merge-2.tif-RGB-1-150x150.jpg 150w, https://focalplane.biologists.com/wp-content/uploads/2024/08/David-Gaboriau_MAX_Image-4_Structured-Illumination_zcrop_MIP_fluo_merge-2.tif-RGB-1-768x768.jpg 768w, https://focalplane.biologists.com/wp-content/uploads/2024/08/David-Gaboriau_MAX_Image-4_Structured-Illumination_zcrop_MIP_fluo_merge-2.tif-RGB-1-1536x1536.jpg 1536w, https://focalplane.biologists.com/wp-content/uploads/2024/08/David-Gaboriau_MAX_Image-4_Structured-Illumination_zcrop_MIP_fluo_merge-2.tif-RGB-1-2048x2048.jpg 2048w" sizes="(max-width: 300px) 100vw, 300px" data-attachment-id="21630" data-permalink="https://focalplane.biologists.com/imagej1-54funitmicron-4/" data-orig-file="https://focalplane.biologists.com/wp-content/uploads/2024/08/David-Gaboriau_MAX_Image-4_Structured-Illumination_zcrop_MIP_fluo_merge-2.tif-RGB-1.jpg" data-orig-size="2430,2430" data-comments-opened="1" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"ImageJ=1.54f\nunit=micron","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"ImageJ=1.54f\nunit=micron","orientation":"0"}" data-image-title="" data-image-description="" data-image-caption="<p>Human fibroblast labelled for mitochondria (Tom20) in yellow, alpha tubulin in pink and actin in grey.</p> " data-medium-file="https://focalplane.biologists.com/wp-content/uploads/2024/08/David-Gaboriau_MAX_Image-4_Structured-Illumination_zcrop_MIP_fluo_merge-2.tif-RGB-1-300x300.jpg" data-large-file="https://focalplane.biologists.com/wp-content/uploads/2024/08/David-Gaboriau_MAX_Image-4_Structured-Illumination_zcrop_MIP_fluo_merge-2.tif-RGB-1-1024x1024.jpg" /></a> </div> <figcaption class='wp-caption-text gallery-caption' id='gallery-1-21630'> Human fibroblast labelled for mitochondria (Tom20) in yellow, alpha tubulin in pink and actin in grey. </figcaption></figure><figure class='gallery-item'> <div class='gallery-icon landscape'> <a href='https://focalplane.biologists.com/imagej1-54g/'><img loading="lazy" decoding="async" width="300" height="300" src="https://focalplane.biologists.com/wp-content/uploads/2024/06/theresa-wiesner_Neuron-en-culture-300x300.jpg" class="attachment-medium size-medium" alt="" aria-describedby="gallery-1-20649" srcset="https://focalplane.biologists.com/wp-content/uploads/2024/06/theresa-wiesner_Neuron-en-culture-300x300.jpg 300w, https://focalplane.biologists.com/wp-content/uploads/2024/06/theresa-wiesner_Neuron-en-culture-1024x1024.jpg 1024w, https://focalplane.biologists.com/wp-content/uploads/2024/06/theresa-wiesner_Neuron-en-culture-150x150.jpg 150w, https://focalplane.biologists.com/wp-content/uploads/2024/06/theresa-wiesner_Neuron-en-culture-768x768.jpg 768w, https://focalplane.biologists.com/wp-content/uploads/2024/06/theresa-wiesner_Neuron-en-culture.jpg 1152w" sizes="(max-width: 300px) 100vw, 300px" data-attachment-id="20649" data-permalink="https://focalplane.biologists.com/imagej1-54g/" data-orig-file="https://focalplane.biologists.com/wp-content/uploads/2024/06/theresa-wiesner_Neuron-en-culture.jpg" data-orig-size="1152,1152" data-comments-opened="1" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"ImageJ=1.54g","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"ImageJ=1.54g","orientation":"0"}" data-image-title="Neuron" data-image-description="<p>Neuron in dissociated hippocampal culture and was imaged using highly inclined thin illumination microscopy (HiLO). The neuron is transfected with a neurotransmitter (glutamate) sensor and shown in orange. The image was acquired using Nikon Elements software / microscope and post-processing was done using FIJI (ImageJ).</p> " data-image-caption="<p>Neuron – Theresa Wiesner</p> " data-medium-file="https://focalplane.biologists.com/wp-content/uploads/2024/06/theresa-wiesner_Neuron-en-culture-300x300.jpg" data-large-file="https://focalplane.biologists.com/wp-content/uploads/2024/06/theresa-wiesner_Neuron-en-culture-1024x1024.jpg" /></a> </div> <figcaption class='wp-caption-text gallery-caption' id='gallery-1-20649'> Neuron – Theresa Wiesner </figcaption></figure><figure class='gallery-item'> <div class='gallery-icon landscape'> <a href='https://focalplane.biologists.com/selected_joe-mckellar_boa-kidney-syncytium_21/'><img loading="lazy" decoding="async" width="300" height="300" src="https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Joe-McKellar_Boa-kidney-syncytium_21-300x300.jpg" class="attachment-medium size-medium" alt="" aria-describedby="gallery-1-20348" srcset="https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Joe-McKellar_Boa-kidney-syncytium_21-300x300.jpg 300w, https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Joe-McKellar_Boa-kidney-syncytium_21-1024x1024.jpg 1024w, https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Joe-McKellar_Boa-kidney-syncytium_21-150x150.jpg 150w, https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Joe-McKellar_Boa-kidney-syncytium_21-768x768.jpg 768w, https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Joe-McKellar_Boa-kidney-syncytium_21-1536x1536.jpg 1536w, https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Joe-McKellar_Boa-kidney-syncytium_21-2048x2048.jpg 2048w" sizes="(max-width: 300px) 100vw, 300px" data-attachment-id="20348" data-permalink="https://focalplane.biologists.com/selected_joe-mckellar_boa-kidney-syncytium_21/" data-orig-file="https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Joe-McKellar_Boa-kidney-syncytium_21-scaled.jpg" data-orig-size="2560,2560" data-comments-opened="1" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="Boa constrictor kidney syncytium" data-image-description="<p>This image is that of a cell culture of boa constrictor kidney cells that are overexpressing a viral fusogenic glycoprotein (VSV-G), which causes cells to fuse together, forming a syncytium. It was acquired using an LSM 980 8Y confocal with an Airyscan 2 module. The image has been pre-processed in the Zen Blue software with post-processing using FIJI. F-actin = gold; cellular DNA = cyan and VSV-G = white.</p> " data-image-caption="<p>Boa constrictor kidney syncytium – Joe McKellar</p> " data-medium-file="https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Joe-McKellar_Boa-kidney-syncytium_21-300x300.jpg" data-large-file="https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Joe-McKellar_Boa-kidney-syncytium_21-1024x1024.jpg" /></a> </div> <figcaption class='wp-caption-text gallery-caption' id='gallery-1-20348'> Boa constrictor kidney syncytium – Joe McKellar </figcaption></figure><figure class='gallery-item'> <div class='gallery-icon portrait'> <a href='https://focalplane.biologists.com/basic-rgb/'><img loading="lazy" decoding="async" width="158" height="300" src="https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Marina-Schernthanner_Image001_8-158x300.jpg" class="attachment-medium size-medium" alt="" aria-describedby="gallery-1-20347" srcset="https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Marina-Schernthanner_Image001_8-158x300.jpg 158w, https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Marina-Schernthanner_Image001_8-540x1024.jpg 540w, https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Marina-Schernthanner_Image001_8-79x150.jpg 79w, https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Marina-Schernthanner_Image001_8-768x1456.jpg 768w, https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Marina-Schernthanner_Image001_8-810x1536.jpg 810w, https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Marina-Schernthanner_Image001_8.jpg 1063w" sizes="(max-width: 158px) 100vw, 158px" data-attachment-id="20347" data-permalink="https://focalplane.biologists.com/basic-rgb/" data-orig-file="https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Marina-Schernthanner_Image001_8.jpg" data-orig-size="1063,2015" data-comments-opened="1" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"Basic RGB","created_timestamp":"1715641584","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"Basic RGB","orientation":"0"}" data-image-title="Mouse hair follicles in cleared postnatal skin tissue" data-image-description="<p>3D whole-mount immunofluorescence image of cleared murine skin tissue (postnatal day 9), side view. Keratin 14 (basal epidermal cells) in red, P-Cadherin (keratinocytes) in green, LYVE-1 (lymphatics) in yellow and DAPI (nuclei) in blue. The image was acquired using an Andor Dragonfly spinning disk microscope and processed in Imaris 9.5.1.</p> " data-image-caption="<p>Mouse hair follicles in cleared postnatal skin tissue – Marina Schernthanner</p> " data-medium-file="https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Marina-Schernthanner_Image001_8-158x300.jpg" data-large-file="https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Marina-Schernthanner_Image001_8-540x1024.jpg" /></a> </div> <figcaption class='wp-caption-text gallery-caption' id='gallery-1-20347'> Mouse hair follicles in cleared postnatal skin tissue – Marina Schernthanner </figcaption></figure><figure class='gallery-item'> <div class='gallery-icon landscape'> <a href='https://focalplane.biologists.com/selected_lorna-young_skeleton_4/'><img loading="lazy" decoding="async" width="300" height="300" src="https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Lorna-Young_Skeleton_4-300x300.jpg" class="attachment-medium size-medium" alt="" aria-describedby="gallery-1-20346" srcset="https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Lorna-Young_Skeleton_4-300x300.jpg 300w, https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Lorna-Young_Skeleton_4-150x150.jpg 150w, https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Lorna-Young_Skeleton_4-768x768.jpg 768w, https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Lorna-Young_Skeleton_4.jpg 926w" sizes="(max-width: 300px) 100vw, 300px" data-attachment-id="20346" data-permalink="https://focalplane.biologists.com/selected_lorna-young_skeleton_4/" data-orig-file="https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Lorna-Young_Skeleton_4.jpg" data-orig-size="926,926" data-comments-opened="1" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"1594663180","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="The skeleton" data-image-description="<p>U2OS cell stained with actin (magenta), microtubules (yellow) and vimentin (intermediate filaments). Imaged on Zeiss 900 Airyscan.</p> " data-image-caption="<p>The skeleton – Lorna Young</p> " data-medium-file="https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Lorna-Young_Skeleton_4-300x300.jpg" data-large-file="https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Lorna-Young_Skeleton_4.jpg" /></a> </div> <figcaption class='wp-caption-text gallery-caption' id='gallery-1-20346'> The skeleton – Lorna Young </figcaption></figure><figure class='gallery-item'> <div class='gallery-icon landscape'> <a href='https://focalplane.biologists.com/imagej1-54f-2/'><img loading="lazy" decoding="async" width="300" height="300" src="https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Ioakeim-Ampartzidis_Mosaic_25-300x300.jpg" class="attachment-medium size-medium" alt="" aria-describedby="gallery-1-20345" srcset="https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Ioakeim-Ampartzidis_Mosaic_25-300x300.jpg 300w, https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Ioakeim-Ampartzidis_Mosaic_25-150x150.jpg 150w, https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Ioakeim-Ampartzidis_Mosaic_25-768x768.jpg 768w, https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Ioakeim-Ampartzidis_Mosaic_25.jpg 992w" sizes="(max-width: 300px) 100vw, 300px" data-attachment-id="20345" data-permalink="https://focalplane.biologists.com/imagej1-54f-2/" data-orig-file="https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Ioakeim-Ampartzidis_Mosaic_25.jpg" data-orig-size="992,992" data-comments-opened="1" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"ImageJ=1.54f","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"ImageJ=1.54f","orientation":"0"}" data-image-title="Mosaic" data-image-description="<p>In this image, the actin cytoskeleton of neuroepithelial cells is shown in blue, and the membranes of some randomly selected cells are highlighted in cyan using synthetic fluorescent labelling dyes. To emphasize the cellular variability within our neuroepithelial sheets, the apical membranes of cells were pseudo-coloured based on their size, ranging from purple (small apical area) to white (large).</p> " data-image-caption="<p>Mosaic – Ioakeim (Makis) Ampartzidis</p> " data-medium-file="https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Ioakeim-Ampartzidis_Mosaic_25-300x300.jpg" data-large-file="https://focalplane.biologists.com/wp-content/uploads/2024/06/Selected_Ioakeim-Ampartzidis_Mosaic_25.jpg" /></a> </div> <figcaption class='wp-caption-text gallery-caption' id='gallery-1-20345'> Mosaic – Ioakeim (Makis) Ampartzidis </figcaption></figure> </div> <script> var image_data_23501 = ['Md Hashim Reza', '', '', '', 'Cover Image', '']; var image_data_23495 = ['Sanja Sviben/Jing Hughes', '', '', '', 'JOCES-2024-262038 cover candidate', '']; var image_data_23489 = ['Jiwon Lee', '', '', '', 'organelles_plasmodium', '']; var image_data_21630 = ['David Gaboriau', '', '', '', '', '']; var image_data_20649 = ['Theresa Wiesner', '', '', '', 'Neuron', 'Neuron in dissociated hippocampal culture and was imaged using highly inclined thin illumination microscopy (HiLO). The neuron is transfected with a neurotransmitter (glutamate) sensor and shown in orange. The image was acquired using Nikon Elements software / microscope and post-processing was done using FIJI (ImageJ).']; var image_data_20348 = ['Joe McKellar', '', '', '', 'Boa constrictor kidney syncytium', 'This image is that of a cell culture of boa constrictor kidney cells that are overexpressing a viral fusogenic glycoprotein (VSV-G), which causes cells to fuse together, forming a syncytium. It was acquired using an LSM 980 8Y confocal with an Airyscan 2 module. The image has been pre-processed in the Zen Blue software with post-processing using FIJI. F-actin = gold; cellular DNA = cyan and VSV-G = white.']; var image_data_20347 = ['Marina Schernthanner', '', '', '', 'Mouse hair follicles in cleared postnatal skin tissue', '3D whole-mount immunofluorescence image of cleared murine skin tissue (postnatal day 9), side view. 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