LDA Cell Counting Suite

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[{"playIfBase":"0","showRelated":"0","videoAutoRestart":"0"}], "optionsVideoData": [] } } } </script> <div class="action-skip-wrapper"><a class="action skip gallery-prev-area" href="#gallery-prev-area"> <span> Skip to the beginning of the images gallery </span> </a> </div><a id="gallery-next-area" tabindex="-1"></a> </div></div></div></div></div><div class="column main container"><div class="product-info-main"><div class="product-info-price"><div class="product-info-stock-sku"> </div></div> <div class="product attribute overview"> <div class="value" itemprop="description"><div class="row mt-4"> <div class="col-md-10"> <p>Enabled by CTL’s LDA Cell Counting Suite such ImmunoSpot® Readers can do LDA counting of PBMC samples (or of any other cell type) in various hemocytometer formats and in 96 well plates. LDA counting of 96 PBMC samples in a plate takes just minutes including automatic calculations of resuspension volumes and generation of a concise report <a href="/example-of-lda-output">(see example).</a></p> <p>LDA counting is important for the assessment of PBMC samples’ fitness following cryopreservation or prolonged storage/shipment <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4381208/" target="_blank" rel="noreferrer noopener">[ref]</a>. The accurate counting of live cells in PBMC is essential for adjusting the cell numbers to be plated into ELISPOT/FluoroSpot assays as divergent live cell counts contribute the largest variability to inter-assay spot count reproducibility. Unless using a dedicated instrument for live, dead, and apoptotic cell counting, this process is time-consuming and rate limiting for testing of multiple PBMC samples in an experiment.</p> <p>CTL’s philosophy is to offer instruments that support several functions commonly performed in immune monitoring laboratories. Enabled by the LDA Suite, ImmunoSpot® Readers are rendered high-end cell counters, saving our customers the expense of having to purchase and service a dedicated cell counter. All necessary functions are included in the cost of a single instrument.</p> <p><a href="/regulatory-compliance-of-immunospot-readers">Learn more</a> about the regulatory compliance of the ImmunoSpot® Software.</p> </div> </div></div> </div> <div class="mp-attachment-container"> <h2>Documents</h2> <div class="mp-attachment-tab"> <ul> <li class="mp-attachment-tab__item"> <a target="_blank" href="https://immunospot.com/mpattachments/file/viewonline/id/389/product_id/797/" title="microarray_presentation"> <img src="https://immunospot.com/static/version1728550059/frontend/Forti/immuno/en_US/Mageplaza_ProductAttachments/media/icons/file-default.png" alt="microarray_presentation"/> <span class="mp-attachment-tab__item__name"> microarray_presentation (554.88 KB) </span> </a> </li> <li class="mp-attachment-tab__item"> <a target="_blank" href="https://immunospot.com/mpattachments/file/viewonline/id/390/product_id/797/" title="scanning_microarrays_10-3"> <img src="https://immunospot.com/static/version1728550059/frontend/Forti/immuno/en_US/Mageplaza_ProductAttachments/media/icons/file-default.png" alt="scanning_microarrays_10-3"/> <span class="mp-attachment-tab__item__name"> scanning_microarrays_10-3 (218.12 KB) </span> </a> </li> <li class="mp-attachment-tab__item"> <a target="_blank" href="https://immunospot.com/mpattachments/file/viewonline/id/391/product_id/797/" title="96-well-counting"> <img src="https://immunospot.com/static/version1728550059/frontend/Forti/immuno/en_US/Mageplaza_ProductAttachments/media/icons/file-default.png" alt="96-well-counting"/> <span class="mp-attachment-tab__item__name"> 96-well-counting (245.32 KB) </span> </a> </li> </ul> </div> <style> .mp-attachment-tab { margin-bottom: 25px; } .mp-attachment-tab ul { list-style-type: none; padding: 0; margin: 0; } .mp-attachment-tab__item { position: relative; margin-bottom: 1.5rem; margin-left: 10px; } .mp-attachment-tab__item img { width: 25px; } 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<img class="rounded-3" src="https://immunospot.com/media/images/software/lda-cell-counting/Donor-1-live-cells.jpg" alt="Donor-1-live-cells" /> <h5 class="position-absolute caption bottom">Donor 1 live cells</h5> </div> <div class="item"> <h3 class="delay-3 fade-in position-absolute caption">99 counted</h3> <img class="rounded-3" src="https://immunospot.com/media/images/software/lda-cell-counting/Donor-1-dead-cells.jpg" alt="Donor-1-dead-cells" /> <h5 class="position-absolute caption bottom">Donor 1 dead cells</h5> </div> <div class="item"> <h3 class="delay-3 fade-in position-absolute caption">78 counted</h3> <img class="rounded-3" src="https://immunospot.com/media/images/software/lda-cell-counting/Donor-1-apoptotic-cells.jpg" alt="Donor-1-apoptotic-cells.jpg" /> <h5 class="position-absolute caption bottom">Donor 1 apoptotic cells</h5> </div> <div class="item"> <h3 class="delay-3 fade-in position-absolute caption">1122 counted</h3> <img src="https://immunospot.com/media/images/software/lda-cell-counting/Donor-2-live-cells.jpg" alt="Donor-2-live-cells" /> <h5 class="position-absolute caption bottom">Donor 2 live cells</h5> </div> <div class="item"> <h3 class="delay-3 fade-in position-absolute caption">125 counted</h3> <img class="rounded-3" src="https://immunospot.com/media/images/software/lda-cell-counting/Donor-2-dead-cells.jpg" alt="Donor-2-dead-cells" /> <h5 class="position-absolute caption bottom">Donor 2 dead cells</h5> </div> <div class="item"> <h3 class="delay-3 fade-in position-absolute caption">6 counted</h3> <img class="rounded-3" src="https://immunospot.com/media/images/software/lda-cell-counting/Donor-2-apoptotic-cells.jpg" alt="Donor-2-apoptotic-cells" /> <h5 class="position-absolute caption bottom">Donor 2 apoptotic cells</h5> </div> <div class="item"> <h3 class="delay-3 fade-in position-absolute caption">866 counted</h3> <img class="rounded-3" 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document.getElementById('Chart6'); var Chart6 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-446', data: [80, 75, 59, 42, 21, 19, 15], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart7').length > 0) { var ctx = document.getElementById('Chart7'); var Chart7 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-438', data: [62, 31, 19, 9, 3, 2, -8], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart8').length > 0) { var ctx = document.getElementById('Chart8'); var Chart8 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-435', data: [59, 33, 13, 9, 1, 1, -2], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart9').length > 0) { var ctx = document.getElementById('Chart9'); var Chart9 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-441', data: [72, 40, 17, 7, 8, 1], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart10').length > 0) { var ctx = document.getElementById('Chart10'); var Chart10 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-433', data: [57, 48, 22, 13, 7, 3, 0], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart11').length > 0) { var ctx = document.getElementById('Chart11'); var Chart11 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-425', data: [54, 35, 10, 8, 1, 2, 1], fill: false, borderColor: 'rgb(75, 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"", "", ""], datasets: [{ label: 'LP-406', data: [63, 39, 23, 14, 4, 5, 5], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart14').length > 0) { var ctx = document.getElementById('Chart14'); var Chart14 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-404', data: [60, 35, 25, 14, 5, 8, 3], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart15').length > 0) { var ctx = document.getElementById('Chart15'); var Chart15 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-401', data: [65, 38, 18, 15, 5, 8, 3], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart16').length > 0) { var ctx = document.getElementById('Chart16'); var Chart16 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-394', data: [50, 28, 18, 9, 3, 1, 1], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart17').length > 0) { var ctx = document.getElementById('Chart17'); var Chart17 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-393', data: [73, 44, 24, 15, 8, 4, 1], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart18').length > 0) { var ctx = document.getElementById('Chart18'); var Chart18 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-391', data: [75, 49, 31, 18, 11, 3], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart19').length > 0) { var ctx = document.getElementById('Chart19'); var Chart19 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-390', data: [60, 38, 21, 12, 10, 5], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart20').length > 0) { var ctx = document.getElementById('Chart20'); var Chart20 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-387', data: [72, 39, 24, 12, 5, 4, 6], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart21').length > 0) { var ctx = document.getElementById('Chart21'); var Chart21 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-386', data: [52, 34, 18, 11, 3, 4, 5], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart22').length > 0) { var ctx = document.getElementById('Chart22'); var Chart22 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-385', data: [58, 29, 19, 12, 3, 0, 4], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart23').length > 0) { var ctx = document.getElementById('Chart23'); var Chart23 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-381', data: [80, 68, 42, 23, 11, 5, 0], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart24').length > 0) { var ctx = document.getElementById('Chart24'); var Chart24 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-372', data: [80, 65, 43, 30, 14, 10, 8], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart25').length > 0) { var ctx = document.getElementById('Chart25'); var Chart25 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-371', data: [68, 33, 20, 8, 5, 1, -8], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart26').length > 0) { var ctx = document.getElementById('Chart26'); var Chart26 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-368', data: [74, 49, 27, 18, 9, 2, 1], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart27').length > 0) { var ctx = document.getElementById('Chart27'); var Chart27 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-367', data: [80, 61, 41, 27, 14, 3, 1], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart28').length > 0) { var ctx = document.getElementById('Chart28'); var Chart28 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-366', data: [56, 37, 22, 8, 2, -1, -1], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart29').length > 0) { var ctx = document.getElementById('Chart29'); var Chart29 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-355', data: [75, 69, 44, 21, 11, 8, 2], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart30').length > 0) { var ctx = document.getElementById('Chart30'); var Chart30 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP-353', data: [59, 39, 18, 9, 2, 7, -1], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart31').length > 0) { var ctx = document.getElementById('Chart31'); var Chart31 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP349', data: [59, 37, 18, 2, -2, -3, -4], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart32').length > 0) { var ctx = document.getElementById('Chart32'); var Chart32 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP341', data: [56, 40, 19, 10, 7, 2, -2], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } if (document.querySelectorAll('#Chart33').length > 0) { var ctx = document.getElementById('Chart33'); var Chart33 = new Chart(ctx, { type: 'line', data: { labels: ["", "", "", "", "", "", ""], datasets: [{ label: 'LP98', data: [73, 53, 40, 25, 15, 9, 2], fill: false, borderColor: 'rgb(75, 192, 192)', tension: 0.1, }] }, options: { responsive: true, interaction: { intersect: false, }, scales: { x: { display: true, title: { display: true, text: 'Effector: Target Ratio' }, }, y: { display: true, title: { display: true, text: '%Killing' }, } } }, }); } }); </script> <script> require(['jquery', 'tinyslider'], function() { if (document.querySelectorAll('.front-header-slider').length > 0) { const front = tns({ "container": ".front-header-slider", "controls": false, "navPosition": "bottom", "autoplay": true, "autoplayButtonOutput": false, "autoplayTimeout": 3000, "autoplayHoverPause": false }); } if (document.querySelectorAll('.well-soft-slider').length > 0) { const well = tns({ "container": 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