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Transcriptome Analysis of Protestia brevitarsis seulensis with Focus On Wing Development and Metamorphosis in Developmental Stages
<!DOCTYPE html> <html lang="en" dir="ltr"> <head> <!-- Google tag (gtag.js) --> <script async src="https://www.googletagmanager.com/gtag/js?id=G-P63WKM1TM1"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-P63WKM1TM1'); </script> <!-- Yandex.Metrika counter --> <script type="text/javascript" > (function(m,e,t,r,i,k,a){m[i]=m[i]||function(){(m[i].a=m[i].a||[]).push(arguments)}; m[i].l=1*new Date(); for (var j = 0; j < document.scripts.length; j++) {if (document.scripts[j].src === r) { return; }} k=e.createElement(t),a=e.getElementsByTagName(t)[0],k.async=1,k.src=r,a.parentNode.insertBefore(k,a)}) (window, document, "script", "https://mc.yandex.ru/metrika/tag.js", "ym"); ym(55165297, "init", { clickmap:false, trackLinks:true, accurateTrackBounce:true, webvisor:false }); </script> <noscript><div><img src="https://mc.yandex.ru/watch/55165297" style="position:absolute; left:-9999px;" alt="" /></div></noscript> <!-- /Yandex.Metrika counter --> <!-- Matomo --> <!-- End Matomo Code --> <title>Transcriptome Analysis of Protestia brevitarsis seulensis with Focus On Wing Development and Metamorphosis in Developmental Stages</title> <meta name="description" content="Transcriptome Analysis of Protestia brevitarsis seulensis with Focus On Wing Development and Metamorphosis in Developmental Stages"> <meta name="keywords" content="white-spotted flower chafers, transcriptomics, RNA-seq, network biology, wing development, metamorphosis"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link 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href="https://publications.waset.org/abstracts/search?q=Chorong%20Shin"> Chorong Shin</a>, <a href="https://publications.waset.org/abstracts/search?q=Joon%20Ha%20Lee"> Joon Ha Lee</a>, <a href="https://publications.waset.org/abstracts/search?q=Jae-Sam%20Hwang"> Jae-Sam Hwang</a>, <a href="https://publications.waset.org/abstracts/search?q=Ui%20Wook%20Hwang"> Ui Wook Hwang</a> </p> <p class="card-text"><strong>Abstract:</strong></p> White-spotted flower chafers are widely distributed in Asian countries and traditionally used for the treatment of chronic fatigue, blood circulation, and paralysis in the oriental medicine field. The evolution and development of insect wings and metamorphosis remain under-discovered subjects in arthropod evolutionary researches. Gene expression abundance analyses along with developmental stages based on the large-scale RNA-seq data are also still rarely done. Here we report the de novo assembly of a Protestia brevitarsis seulensis transcriptome along four different developmental stages (egg, larva, pupa, and adult) to explore its development and evolution of wings and metamorphosis. The de novo transcriptome assembly consists of 23,551 high-quality transcripts and is approximately 96.7% complete. Out of 8,545 transcripts, 5,183 correspond to the possible orthologs with Drosophila melanogaster. As a result, we could found 265 genes related to wing development and 19 genes related to metamorphosis. The comparison of transcript expression abundance with different developmental stages revealed developmental stage-specific transcripts especially working at the stage of wing development and metamorphosis of P. b. seulensis. This transcriptome quantification along the developmental stages may provide some meaningful clues to elucidate the genetic modulation mechanism of wing development and metamorphosis obtained during the insect evolution. <iframe src="https://publications.waset.org/abstracts/138739.pdf" style="width:100%; height:400px;" frameborder="0"></iframe> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=white-spotted%20flower%20chafers" title="white-spotted flower chafers">white-spotted flower chafers</a>, <a href="https://publications.waset.org/abstracts/search?q=transcriptomics" title=" transcriptomics"> transcriptomics</a>, <a href="https://publications.waset.org/abstracts/search?q=RNA-seq" title=" RNA-seq"> RNA-seq</a>, <a href="https://publications.waset.org/abstracts/search?q=network%20biology" title=" network biology"> network biology</a>, <a href="https://publications.waset.org/abstracts/search?q=wing%20development" title=" wing development"> wing development</a>, <a href="https://publications.waset.org/abstracts/search?q=metamorphosis" title=" metamorphosis"> metamorphosis</a> </p> <a href="https://publications.waset.org/abstracts/138739/transcriptome-analysis-of-protestia-brevitarsis-seulensis-with-focus-on-wing-development-and-metamorphosis-in-developmental-stages" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/138739.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">229</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> 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