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class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 1559</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: endophytic bacteria</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1559</span> Antibacterial Activity of Endophytic Bacteria against Multidrug-Resistant Bacteria: Isolation, Characterization, and Antibacterial Activity</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Maryam%20Beiranvand">Maryam Beiranvand</a>, <a href="https://publications.waset.org/abstracts/search?q=Sajad%20Yaghoubi"> Sajad Yaghoubi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Some microbes can colonize plants’ inner tissues without causing obvious damage and can even produce useful bioactive substances. In the present study, the diversity of the endophytic bacteria associated with medicinal plants from Iran was investigated by culturing techniques, molecular gene identification, as well as measuring them for antibacterial activity. Results: In the spring season from 2013 to 2014, 35 herb pharmacology samples were collected, sterilized, meshed, and then cultured on selective media culture. A total of 199 endophytic bacteria were successfully isolated from 35 tissue cultures of medical plants, and sixty-seven out of 199 bacterial isolates were subjected to identification by the 16S rRNA gene sequence analysis method. Based on the sequence similarity gene and phylogenetic analyses, these isolates were grouped into five classes, fourteen orders, seventeen families, twenty-one genera, and forty strains. The most abundant group of endophytic bacteria was actinobacterial, consisting of thirty-two (47%) out of 67 bacterial isolates. Ten (22.3%) out of 67 bacterial isolates remained unidentiﬁed and classified at the genus level. The signature of the 16S rRNA gene formed a distinct line in a phylogenetic tree showing that they might be new species of bacteria. One (5.2%) out of 67 bacterial isolates was still not well categorized. Forty-two out of 67 strains were candidates for antimicrobial activity tests. Nineteen (45%) out of 42 strains showed antimicrobial activity multidrug resistance (MDR); thirteen (68%) out of 19 strains were allocated to classes actinobacteria. Four (21%) out of 19 strains belonged to the Bacillaceae family, one (5.2%) out of 19 strains was the Paenibacillaceae family, and one (5.2%) out of 19 strains belonged to the Pseudomonadaceae family. The other twenty-three strains did not show inhibitory activities. Conclusions: Our research showed a high-level phylogenetic diversity and the intoxicating antibiotic activity of endophytic bacteria in the herb pharmacology of Iran. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Antibacterial%20activity" title="Antibacterial activity">Antibacterial activity</a>, <a href="https://publications.waset.org/abstracts/search?q=endophytic%20bacteria" title=" endophytic bacteria"> endophytic bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=multidrug-resistant%20bacteria" title=" multidrug-resistant bacteria"> multidrug-resistant bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=whole%20genom%20sequencing" title=" whole genom sequencing"> whole genom sequencing</a> </p> <a href="https://publications.waset.org/abstracts/164258/antibacterial-activity-of-endophytic-bacteria-against-multidrug-resistant-bacteria-isolation-characterization-and-antibacterial-activity" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/164258.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">89</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1558</span> Morphological and Molecular Identification of Endophytic Colletotrichum Species from Medicinal Plants and Their Antimicrobial Potential</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gauravi%20Agarkar">Gauravi Agarkar</a>, <a href="https://publications.waset.org/abstracts/search?q=Mahendra%20Rai"> Mahendra Rai</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Endophytic fungi from medicinal plants are important source of numerous pharmacologically important compounds. In the present investigation, the endophytic fungi were isolated from three medicinal plants; Andrographis paniculata, Rauwolfia serpentina and Tridax procumbens. Endophytic Colletotrichum sp. were identified on the basis of cultural and morphological characteristics as well as internal transcribed spacer (ITS) sequence analysis. Antibacterial and antifungal activity of the ethyl acetate and methanol extract of endophytic Colletotrichum sp. was evaluated against seven different human pathogenic bacteria and six Candida sp. The extracts were effective and showed significant activity against all the test pathogens. In case of yeast Candida, the combined effect of extracts and standard antibiotic was enhanced greatly showing synergistic activity. Further, the extracts were assayed for Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal/Fungicidal Concentration (MBC/MFC) where, MIC values were in the range of 100-250 μg/ml. These results suggest that the endophytic Colletotrichum sp. isolated from the medicinal plants are capable of producing promising antimicrobial metabolites. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antimicrobial" title="antimicrobial">antimicrobial</a>, <a href="https://publications.waset.org/abstracts/search?q=colletotrichum" title=" colletotrichum"> colletotrichum</a>, <a href="https://publications.waset.org/abstracts/search?q=endophytic%20fungi" title=" endophytic fungi"> endophytic fungi</a>, <a href="https://publications.waset.org/abstracts/search?q=medicinal%20plants" title=" medicinal plants"> medicinal plants</a> </p> <a href="https://publications.waset.org/abstracts/20309/morphological-and-molecular-identification-of-endophytic-colletotrichum-species-from-medicinal-plants-and-their-antimicrobial-potential" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/20309.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">563</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1557</span> Isolation, Characterization, and Antibacterial Activity of Endophytic Bacteria from Iranian Medicinal Plants</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Maryam%20Beiranvand">Maryam Beiranvand</a>, <a href="https://publications.waset.org/abstracts/search?q=Sajad%20Yaghoubi"> Sajad Yaghoubi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Some microbes can colonize plants’ inner tissues without causing obvious damage and can even produce useful bioactive substances. In the present study, the diversity of the endophytic bacteria associated with medicinal plants from Iran was investigated by culturing techniques, molecular gene identification, as well as measuring them for antibacterial activity. Results: In the spring season from 2013 to 2014, 35 herb pharmacology samples were collected, sterilized, meshed, and then cultured on selective media culture. A total of 199 endophytic bacteria were successfully isolated from 35 tissue cultures of medical plants, and sixty-seven out of 199 bacterial isolates were subjected to identification by the 16S rRNA gene sequence analysis method. Based on the sequence similarity gene and phylogenetic analyses, these isolates were grouped into five classes, fourteen orders, seventeen families, twenty-one genera, and forty strains. The most abundant group of endophytic bacteria was actinobacterial, consisting of thirty-two (47%) out of 67 bacterial isolates. Ten (22.3%) out of 67 bacterial isolates remained unidentiﬁed and classified at the genus level. The signature of the 16S rRNA gene formed a distinct line in a phylogenetic tree showing that they might be new species of bacteria. One (5.2%) out of 67 bacterial isolates was still not well categorized. Forty-two out of 67 strains were candidates for antimicrobial activity tests. Nineteen (45%) out of 42 strains showed antimicrobial activity multidrug-resistance (MDR); thirteen (68%) out of 19 strains were allocated to classes actinobacteria. Four (21%) out of 19 strains belonged to the Bacillaceae family, one (5.2%) out of 19 strains was the Paenibacillaceae family, and one (5.2%) out of 19 strains belonged to the Pseudomonadaceae family. The other twenty-three strains did not show inhibitory activities. Conclusions: Our research showed a high-level phylogenetic diversity and the intoxicating antibiotic activity of endophytic bacteria in the herb pharmacology of Iran. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=medical%20plant" title="medical plant">medical plant</a>, <a href="https://publications.waset.org/abstracts/search?q=endophytic%20bacteria" title=" endophytic bacteria"> endophytic bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=antimicrobial%20activity" title=" antimicrobial activity"> antimicrobial activity</a>, <a href="https://publications.waset.org/abstracts/search?q=whole%20genome%20sequencing%20analysis" title=" whole genome sequencing analysis"> whole genome sequencing analysis</a> </p> <a href="https://publications.waset.org/abstracts/164252/isolation-characterization-and-antibacterial-activity-of-endophytic-bacteria-from-iranian-medicinal-plants" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/164252.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">127</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1556</span> Isolation and Characterization of Endophytic Bacteria Associated with Root-Nodules of Medicago sativa in Al-Ahasa Region</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ashraf%20Y.%20Z.%20Khalifa">Ashraf Y. Z. Khalifa</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammed%20A.%20Almalki"> Mohammed A. Almalki</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Medicago sativa (Alfalfa) is an important forage crop legume worldwide including Saudia Arabia due to its high nutritive value. Soil bacteria exist in root or root-nodules of Medicago sativa in either symbiotic relationships or in associations. The aim of the present study was to isolate and characterize endophytic bacteria that live in association with non-nodulated roots of Medicago sativa growing in Al-Ahsaa region, Saudia Arabia. Several bacterial strains were isolated from sterilized roots of Medicago sativa. Strains were characterized using 16S rRNA gene sequences, phylogenetic relationships analysis, morphological and biochemical characteristics. The strains utilized 50% (10 out of 20) of the different chemical substrates contained in the API20E strip. In general, many strains had the ability to ferment/oxidise all the carbohydrate tested except for rhamnose and the polyol carbohydrate, inositol. Comparative sequence analysis of the 16S rDNA gene indicated that the strains were closely related to the genus Bacillus. Furthermore, the growth parameters of Vigna sinensis were enhanced upon single-inoculation of the isolated strains, compared to the uninoculated control plants. The results highlighted that the root-nodules of Medicago sativa harbor non-nodulating bacterial strains that could have significant agricultural applications. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Medicago%20sativa" title="Medicago sativa">Medicago sativa</a>, <a href="https://publications.waset.org/abstracts/search?q=endophytic%20bacteria" title=" endophytic bacteria"> endophytic bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=Pisum%20sativum" title=" Pisum sativum"> Pisum sativum</a>, <a href="https://publications.waset.org/abstracts/search?q=Vigna%20sinensis" title=" Vigna sinensis"> Vigna sinensis</a> </p> <a href="https://publications.waset.org/abstracts/7225/isolation-and-characterization-of-endophytic-bacteria-associated-with-root-nodules-of-medicago-sativa-in-al-ahasa-region" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/7225.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">383</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1555</span> Media Manipulations and the Culture of Beneficial Endophytic Fungi in the Leaves and Stem Bark of Grewia lasiocarpa E. Mey. Ex Harv</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Akwu%20A.%20Nneka">Akwu A. Nneka</a>, <a href="https://publications.waset.org/abstracts/search?q=Naidoo"> Naidoo</a>, <a href="https://publications.waset.org/abstracts/search?q=Yougasphree"> Yougasphree</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A significantly high number of microbes exist in higher plants; these microbes include bacteria, fungi, and actinomycetes. There are reports on the benefits of endophytic fungi and their products of metabolism to the host plant and man, consequently, it is expedient to explore the changes that could arise as a result of manipulating their growth media. Grewia lasiocarpa E. Mey. ex Harv. (Malvaceae) is an indigenous Southern African plant, that belongs to a genus with known medicinal properties. Three media were used to culture the endophytic fungi viz., Potato Dextrose Agar (PDA), Malt Extract Agar (MEA), and Bacteriological Agar (BA) were used singly, and supplemented with three dilutions of the leaves and stem bark extracts. The manipulated growth media composition had a significant effect on the diversity of the isolated fungal populations. Several endophytic fungi were isolated; their distribution and diversity revealed a significant relatedness with the manipulated media. The media supplemented with the plant extracts was observed to give a significant increase in the growth rate and yield of the endophytes. To the best of our knowledge, this is the first study describing the endophytic fungi present in the leaves and stem bark of G. lasiocarpa E. Mey. ex Harv. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Grewia%20lasiocarpa" title="Grewia lasiocarpa">Grewia lasiocarpa</a>, <a href="https://publications.waset.org/abstracts/search?q=plant-based%20extracts" title=" plant-based extracts"> plant-based extracts</a>, <a href="https://publications.waset.org/abstracts/search?q=endophytic%20fungi" title=" endophytic fungi"> endophytic fungi</a>, <a href="https://publications.waset.org/abstracts/search?q=Malvaceae" title=" Malvaceae"> Malvaceae</a> </p> <a href="https://publications.waset.org/abstracts/123827/media-manipulations-and-the-culture-of-beneficial-endophytic-fungi-in-the-leaves-and-stem-bark-of-grewia-lasiocarpa-e-mey-ex-harv" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/123827.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">161</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1554</span> Growth of Albizia in vitro: Endophytic Fungi as Plant Growth Promote of Albizia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Reine%20Suci%20Wulandari">Reine Suci Wulandari</a>, <a href="https://publications.waset.org/abstracts/search?q=Rosa%20Suryantini"> Rosa Suryantini</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Albizia (Paraserianthes falcataria) is a woody plant species that has a high economic value and multifunctional. Albizia is important timber, medicinal plants and can also be used as a plant to rehabilitate critical lands. The demand value of Albizia is increased so that the large quantities and high quality of seeds are required. In vitro propagation techniques are seed propagation that can produce more seeds and quality in a short time. In vitro cultures require growth regulators that can be obtained from biological agents such as endophytic fungi. Endophytic fungi are micro fungi that colonize live plant tissue without producing symptoms or other negative effects on host plants and increase plant growth. The purposes of this research were to isolate and identify endophytic fungi isolated from the root of Albizia and to study the effect of endophytic fungus on the growth of Albizia in vitro. The methods were root isolation, endophytic fungal identification, and inoculation of endophytic fungi to Albizia plants in vitro. Endophytic fungus isolates were grown on PDA media before being inoculated with Albizia sprouts. Incubation is done for 4 (four) weeks. The observed growth parameters were live explant percentage, percentage of explant shoot, and percentage of explant rooted. The results of the research showed that 6 (six) endophytic fungal isolates obtained from the root of Albizia, namely Aspergillus sp., Verticillium sp, Penicillium sp., Trichoderma sp., Fusarium sp., and Acremonium sp. Statistical analysis found that Trichoderma sp. and Fusarium sp. affect in vitro growth of Albizia. Endophytic fungi from the results of this research were potential as plant growth promoting. It can be applied to increase productivity either through increased plant growth and increased endurance of Albizia seedlings to pests and diseases. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Albizia" title="Albizia">Albizia</a>, <a href="https://publications.waset.org/abstracts/search?q=endophytic%20fungi" title=" endophytic fungi"> endophytic fungi</a>, <a href="https://publications.waset.org/abstracts/search?q=propagation" title=" propagation"> propagation</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro" title=" in vitro"> in vitro</a> </p> <a href="https://publications.waset.org/abstracts/74725/growth-of-albizia-in-vitro-endophytic-fungi-as-plant-growth-promote-of-albizia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/74725.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">277</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1553</span> Molecular Profiling and Potential Bioactive Characteristics of Endophytic Fungi Isolated from Leptadenia Pyrotechnica</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Walaa%20Al-Maghraby">Walaa Al-Maghraby</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Endophytes are organisms that colonize internal plant tissues without causing apparent harm to their host. Almost all groups of microorganisms have been found in endophytic association with plants may be fungi. They stimulate the production of secondary metabolites with a diverse range of biological activities. Leptadenia pyrotechnica is a more or less leafless, erect shrub with straight stems which is highly distributed in Saudi Arabia. Four endophytes fungi were isolated from Leptadenia pyrotechnica and identified using 18S ribosomal RNA sequences, which revealed four fungi genuses, namely Aspergillus terreus; Aspergillus welwitschiae; Aspergillus fumigatus and Aspergillus flavus. In this present study, four endophytic fungi from Leptadenia pyrotechnica were used for obtaining crude aqueous and ethyl acetate extracts for antimicrobial screening against 6 human pathogens, the antibacterial tests presented satisfactory results, where the pathogenic bacteria were inhibited by the four extracts tested, except for Escherichia coli that was inhibited by all extracts except ethyl acetate extract of Aspergillus terreus. Analysis of variance showed that the extract produced by endophyte Leptadenia pyrotechnica was the most effective against all bacteria, either gram-negative or positive. However, the extract was not efficient against pathogenic fungi. Therefore, this study indicates that endophytes from medicinal plant Leptadenia pyrotechnica could be potential sources of antibacterial substances. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antimicrobial%20activity" title="antimicrobial activity">antimicrobial activity</a>, <a href="https://publications.waset.org/abstracts/search?q=Aspergillus%20sp" title=" Aspergillus sp"> Aspergillus sp</a>, <a href="https://publications.waset.org/abstracts/search?q=endophytes" title=" endophytes"> endophytes</a>, <a href="https://publications.waset.org/abstracts/search?q=Leptadenia%20pyrotechnica" title=" Leptadenia pyrotechnica"> Leptadenia pyrotechnica</a> </p> <a href="https://publications.waset.org/abstracts/120642/molecular-profiling-and-potential-bioactive-characteristics-of-endophytic-fungi-isolated-from-leptadenia-pyrotechnica" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/120642.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">145</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1552</span> Tomato Endophytes Trichoderma asperellum AAUTLF and Stenotrophomonas maltophilia D1B Exhibits Plant Growth-Promotion and Fusarium Wilt Suppression</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bandana%20Saikia">Bandana Saikia</a>, <a href="https://publications.waset.org/abstracts/search?q=Ashok%20Bhattacharyya"> Ashok Bhattacharyya</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Endophytic microbes and their metabolites positively impact overall plant health, which may have a potential implication in agriculture. In the present study, 177 bacterial endophytes and 57 fungal endophytes were isolated, with the highest recovery rate from tomato roots. A maximum of 112 endophytes were isolated during monsoon, followed by 64 isolates and 58 isolates isolated during pre-monsoon and post-monsoon periods, respectively, indicating the rich diversity in bacterial and fungal endophytes of tomato crops from different locations of Assam, India. Further, the endophytes were evaluated for their antagonistic potential against Fusarium oxysporum f. sp. lycopersici. Fungal endophytic isolate AAUTLF (Endophytic Fungi of Tomato Leaf from Assam Agricultural University, Assam, India area) and bacterial endophyte D1B (Endophytic bacteria of tomato from Dhemiji, India district) showed the highest antifungal activity against the pathogen both in vitro and in vivo. Based on 5.8 rDNA sequence analysis of fungal and 16S rDNA sequence of bacteria endophytes, the most effective fungal and bacterial isolates against FOL were identified as Trichoderma asperellum AAUTLF and Stenotrophomonas maltophilia D1B, respectively. The isolates showed an antagonistic effect against Fusarium oxysporum f.sp. lycopersici in-vitro and reduced the disease index of Fusarium wilt in tomatoes by 64.4% under pot conditions. Trichoderma asperellum AAUTLF produced an antifungal compound viz., 6-pentyl-2H-pyran-2-one, which also possesses growth-promoting characteristics. The bacteria Stenotrophomonas maltophilia D1B produced antifungal compounds, including benzothiazole, oleic acid, phenylacetic acid, and 3-(Hydroxy-phenyl-methyl)-2,3-dimethyl-octan-4-one. This would be of high importance for the source of antagonistic strains and biocontrol of tomato Fusarium wilt, as well as other plant fungal diseases. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=root%20endophytes" title="root endophytes">root endophytes</a>, <a href="https://publications.waset.org/abstracts/search?q=Stemotrophomonas" title=" Stemotrophomonas"> Stemotrophomonas</a>, <a href="https://publications.waset.org/abstracts/search?q=Trichoderma" title=" Trichoderma"> Trichoderma</a>, <a href="https://publications.waset.org/abstracts/search?q=benzothiazole" title=" benzothiazole"> benzothiazole</a>, <a href="https://publications.waset.org/abstracts/search?q=6-pentyl-2H-pyran-2-one" title=" 6-pentyl-2H-pyran-2-one"> 6-pentyl-2H-pyran-2-one</a> </p> <a href="https://publications.waset.org/abstracts/165567/tomato-endophytes-trichoderma-asperellum-aautlf-and-stenotrophomonas-maltophilia-d1b-exhibits-plant-growth-promotion-and-fusarium-wilt-suppression" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/165567.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">77</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1551</span> Isolation and Identification Fibrinolytic Protease Endophytic Fungi from Hibiscus Leaves in Shah Alam</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohd%20Sidek%20Ahmad">Mohd Sidek Ahmad</a>, <a href="https://publications.waset.org/abstracts/search?q=Zainon%20Mohd%20Noor"> Zainon Mohd Noor</a>, <a href="https://publications.waset.org/abstracts/search?q=Zaidah%20Zainal%20Ariffin"> Zaidah Zainal Ariffin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Fibrin degradation is an important part in prevention or treatment of intravascular thrombosis and cardiovascular diseases. Plasmin like fibrinolytic enzymes has given new hope to patient with cardiovascular diseases by treating fibrin aggregation related diseases with traditional plasminogen activator which have many side effects. Various researches involving wide range of sources for production of fibrinolytic proteases, from bacteria, fungi, insects and fermented foods. But few have looked into endophytic fungi as a potential source. Sixteen (16) endophytic fungi were isolated from Hibiscus sp. leaves from six different locations in Shah Alam, Selangor. Only two endophytic fungi, FH3 and S13 showed positive fibrinolytic protease activities. FH3 produced 5.78cm and S13 produced 4.48cm on Skim Milk Agar after 4 days of incubation at 27°C. Fibrinolytic activity was observed; 3.87cm and 1.82cm diameter clear zone on fibrin plate of FH3 and S13 respectively. 18srRNA was done for identification of the isolated fungi with positive fibrinolytic protease. S13 had the highest similarity (100%) to that of Penicillium citrinum strain TG2 and FH3 had the highest similarity (99%) to that of Fusarium sp. FW2PhC1, Fusarium sp. 13002, Fusarium sp. 08006, Fusarium equiseti strain Salicorn 8 and Fungal sp. FCASAn-2. Media composition variation showed the effects of carbon nitrogen on protein concentration, where the decrement of 50% of media composition caused drastic decrease in protease of FH3 from 1.081 to 0.056 and also S13 from 2.946 to 0.198. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=isolation" title="isolation">isolation</a>, <a href="https://publications.waset.org/abstracts/search?q=identification" title=" identification"> identification</a>, <a href="https://publications.waset.org/abstracts/search?q=fibrinolytic%20protease" title=" fibrinolytic protease"> fibrinolytic protease</a>, <a href="https://publications.waset.org/abstracts/search?q=endophytic%20fungi" title=" endophytic fungi"> endophytic fungi</a>, <a href="https://publications.waset.org/abstracts/search?q=Hibiscus%20leaves" title=" Hibiscus leaves"> Hibiscus leaves</a> </p> <a href="https://publications.waset.org/abstracts/15095/isolation-and-identification-fibrinolytic-protease-endophytic-fungi-from-hibiscus-leaves-in-shah-alam" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/15095.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">439</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1550</span> Prevalence and Fungicidal Activity of Endophytic Micromycetes of Plants in Kazakhstan</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Lyudmila%20V.%20Ignatova">Lyudmila V. Ignatova</a>, <a href="https://publications.waset.org/abstracts/search?q=Yelena%20V.%20Brazhnikova"> Yelena V. Brazhnikova</a>, <a href="https://publications.waset.org/abstracts/search?q=Togzhan%20D.%20Mukasheva"> Togzhan D. Mukasheva</a>, <a href="https://publications.waset.org/abstracts/search?q=Ramza%20Zh.%20Berzhanova"> Ramza Zh. Berzhanova</a>, <a href="https://publications.waset.org/abstracts/search?q=Anel%20A.%20Omirbekova"> Anel A. Omirbekova</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Endophytic microorganisms are presented in plants of different families growing in the foothills and piedmont plains of Trans-Ili Alatau. It was found that the maximum number of endophytic micromycetes is typical to the Fabaceae family. The number of microscopic fungi in the roots reached (145.9±5.9)×103 CFU/g of plant tissue; yeasts - (79.8±3.5)×102 CFU/g of plant tissue. Basically, endophytic microscopic fungi are typical for underground parts of plants. In contrast, yeasts more infected aboveground parts of plants. Small amount of micromycetes is typical to inflorescence and fruits. Antagonistic activity of selected micromycetes against Fusarium graminearum, Cladosporium sp., Phytophtora infestans and Botrytis cinerea phytopathogens was detected. Strains with a broad, narrow and limited range of action were identified. For further investigations Rh2 and T7 strains were selected, they are characterized by a broad spectrum of fungicidal activity and they formed the large inhibition zones against phytopathogens. Active antagonists are attributed to the Rhodotorula mucilaginosa and Beauveria bassiana species. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=endophytic%20micromycetes" title="endophytic micromycetes">endophytic micromycetes</a>, <a href="https://publications.waset.org/abstracts/search?q=fungicidal%20activity" title=" fungicidal activity"> fungicidal activity</a>, <a href="https://publications.waset.org/abstracts/search?q=prevalence" title=" prevalence"> prevalence</a>, <a href="https://publications.waset.org/abstracts/search?q=plants" title=" plants"> plants</a> </p> <a href="https://publications.waset.org/abstracts/10202/prevalence-and-fungicidal-activity-of-endophytic-micromycetes-of-plants-in-kazakhstan" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/10202.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">328</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1549</span> Crop Genotype and Inoculum Density Influences Plant Growth and Endophytic Colonization Potential of Plant Growth-Promoting Bacterium Burkholderia phytofirmans PsJN</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Naveed">Muhammad Naveed</a>, <a href="https://publications.waset.org/abstracts/search?q=Sohail%20Yousaf"> Sohail Yousaf</a>, <a href="https://publications.waset.org/abstracts/search?q=Zahir%20Ahmad%20Zahir"> Zahir Ahmad Zahir</a>, <a href="https://publications.waset.org/abstracts/search?q=Birgit%20Mitter"> Birgit Mitter</a>, <a href="https://publications.waset.org/abstracts/search?q=Angela%20Sessitsch"> Angela Sessitsch</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Most bacterial endophytes originate from the soil and enter plants via the roots followed by further spread through the inner tissues. The mechanisms allowing bacteria to colonize plants endophytically are still poorly understood for most bacterial and plant species. Specific bacterial functions are required for plant colonization, but also the plant itself is a determining factor as bacterial ability to establish endophytic populations is very often dependent on the plant genotype (cultivar) and inoculums density. The effect of inoculum density (107, 108, 109 CFU mL-1) of Burkholderia phytofirmans strain PsJN was evaluated on growth and endophytic colonization of different maize and potato cultivars under axenic and natural soil conditions. PsJN inoculation significantly increased maize seedling growth and tuber yield of potato at all inoculum density compared to uninoculated control. Under axenic condition, PsJN inoculation (108 CFU mL-1) significantly improved the germination, root/shoot length and biomass up to 62, 115, 98 and 135% of maize seedling compared to uninoculated control. In case of potato, PsJN inoculation (109 CFU mL-1) showed maximum response and significantly increased root/shoot biomass and tuber yield under natural soil condition. We confirmed that PsJN is able to colonize the rhizosphere, roots and shoots of maize and potato cultivars. The endophytic colonization increased linearly with increasing inoculum density (within a range of 8 x 104 – 3 x 107 CFU mL-1) and were highest for maize (Morignon) and potato (Romina) as compared to other cultivars. Efficient colonization of cv. Morignon and Romina by strain PsJN indicates the specific cultivar colonizing capacity of the bacteria. The findings of the study indicate the non-significant relationship between colonization and plant growth promotion in maize under axenic conditions. However, the inoculum level (109 CFU mL-1) that promoted colonization of rhizosphere and plant interior (endophytic) also best promoted growth and tuber yield of potato under natural soil conditions. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=crop%20genotype" title="crop genotype">crop genotype</a>, <a href="https://publications.waset.org/abstracts/search?q=inoculum%20density" title=" inoculum density"> inoculum density</a>, <a href="https://publications.waset.org/abstracts/search?q=Burkholderia%20phytofirmans%20PsJN" title=" Burkholderia phytofirmans PsJN"> Burkholderia phytofirmans PsJN</a>, <a href="https://publications.waset.org/abstracts/search?q=colonization" title=" colonization"> colonization</a>, <a href="https://publications.waset.org/abstracts/search?q=growth" title=" growth"> growth</a>, <a href="https://publications.waset.org/abstracts/search?q=potato" title=" potato"> potato</a> </p> <a href="https://publications.waset.org/abstracts/20888/crop-genotype-and-inoculum-density-influences-plant-growth-and-endophytic-colonization-potential-of-plant-growth-promoting-bacterium-burkholderia-phytofirmans-psjn" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/20888.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">490</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1548</span> Metagenomics Analysis of Bacteria in Sorghum Using next Generation Sequencing</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kedibone%20Masenya">Kedibone Masenya</a>, <a href="https://publications.waset.org/abstracts/search?q=Memory%20Tekere"> Memory Tekere</a>, <a href="https://publications.waset.org/abstracts/search?q=Jasper%20Rees"> Jasper Rees</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Sorghum is an important cereal crop in the world. In particular, it has attracted breeders due to capacity to serve as food, feed, fiber and bioenergy crop. Like any other plant, sorghum hosts a variety of microbes, which can either, have a neutral, negative and positive influence on the plant. In the current study, regions (V3/V4) of 16 S rRNA were targeted to extensively assess bacterial multitrophic interactions in the phyllosphere of sorghum. The results demonstrated that the presence of a pathogen has a significant effect on the endophytic bacterial community. Understanding these interactions is key to develop new strategies for plant protection. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bacteria" title="bacteria">bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=multitrophic" title=" multitrophic"> multitrophic</a>, <a href="https://publications.waset.org/abstracts/search?q=sorghum" title=" sorghum"> sorghum</a>, <a href="https://publications.waset.org/abstracts/search?q=target%20sequencing" title=" target sequencing"> target sequencing</a> </p> <a href="https://publications.waset.org/abstracts/73720/metagenomics-analysis-of-bacteria-in-sorghum-using-next-generation-sequencing" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/73720.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">290</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1547</span> Metabolic Regulation of Rhizobacteria for Cool-Season Grass Tolerance to Heat Stress</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kashif%20Jaeel">Kashif Jaeel</a>, <a href="https://publications.waset.org/abstracts/search?q=Bingru%20Huang"> Bingru Huang</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Stress-induced accumulation of ethylene exacerbates drought damages in plants, and suppressing stress induction of ethylene may promote plant tolerance to heat stress. The objective of this study was to investigate the effects of endophytic bacteria (Paraburkholderia aspalathi) with 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase enzymes in suppressing ethylene production on plant tolerance to heat stress and underlying physiological mechanisms of P. aspalathi-regulation in creeping bentgrass (Agrostis stolonifera). A novel strain of P. aspalathi, ‘WSF23’, with ACC deaminase activity was used to inoculate the roots of plants (cv. ‘Penncross’) subjected to heat stress in controlled-environment chambers. Inoculation with WSF23 bacteria resulted in improved shoot and root growth during heat stress. The differential changes in metabolite regulation due to the bacterial inoculation could contribute to ACC deamination bacteria-improved heat tolerance in cool-season grass species. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=rhizobacteria" title="rhizobacteria">rhizobacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=grass" title=" grass"> grass</a>, <a href="https://publications.waset.org/abstracts/search?q=heat" title=" heat"> heat</a>, <a href="https://publications.waset.org/abstracts/search?q=plant%20metabolism" title=" plant metabolism"> plant metabolism</a>, <a href="https://publications.waset.org/abstracts/search?q=soil%20bacteria" title=" soil bacteria"> soil bacteria</a> </p> <a href="https://publications.waset.org/abstracts/177551/metabolic-regulation-of-rhizobacteria-for-cool-season-grass-tolerance-to-heat-stress" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/177551.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">71</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1546</span> Identification and Characterization of Antimicrobial Peptides Isolated from Entophytic Bacteria and Their Activity against Multidrug-Resistance Gram-Negative Bacteria in South Korea</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Maryam%20Beiranvand">Maryam Beiranvand</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Multi-drug resistance in various microorganisms has increased globally in many healthcare facilities. Less effective antimicrobial activity of drug therapies for infection control becomes trouble. Since 1980, no new type of antimicrobial drug has been identified, even though combinations of antibiotic drugs have been discovered almost every decade. Between 1981 and 2006, over 70% of novel pharmaceuticals and chemical agents came from natural sources. Microorganisms have yielded almost 22,000 natural compounds. The identification of antimicrobial components from endophytes bacteria could help overcome the threat posed by multi-drug resistant strains. The project aims to analyze and identify antimicrobial peptides isolated from entophytic bacteria and their activity against multidrug-resistant Gram-negative bacteria in South Korea. Endophytic Paenibacillus polymyxa. 4G3 isolated from the plant, Gynura procumbery exhibited considerable antimicrobial activity against Methicillin-resistant Staphylococcus aureus, and Escherichia coli. The Rapid Annotations using Subsystems Technology showed that the total size of the draft genome was 5,739,603bp, containing 5178 genes with 45.8% G+C content. Genome annotation using antiSMASH version 6.0.0 was performed, which predicted the most common types of non-ribosomal peptide synthetase (NRPS) and polyketide synthase (PKS). In this study, diethyl aminoethyl cellulose (DEAEC) resin was used as the first step in purifying for unknown peptides, and then the target protein was identified using hydrophilic and hydrophobic solutions, optimal pH, and step-by-step tests for antimicrobial activity. This crude was subjected to C18 chromatography and elution with 0, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100% methanol, respectively. Only the fraction eluted with 20% -60% methanol demonstrated good antimicrobial activity against MDR E. coli. The concentration of the active fragment was measured by the Brad-ford test, and Protein A280 - Thermo Fisher Scientific at the end by examining the SDS PAGE Resolving Gel, 10% Acrylamide and purity were confirmed. Our study showed that, based on the combined results of the analysis and purification. P polymyxa. 4G3 has a high potential exists for producing novel functions of polymyxin E and bacitracin against bacterial pathogens. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=endophytic%20bacteria" title="endophytic bacteria">endophytic bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=antimicrobial%20activity" title=" antimicrobial activity"> antimicrobial activity</a>, <a href="https://publications.waset.org/abstracts/search?q=antimicrobial%20peptide" title=" antimicrobial peptide"> antimicrobial peptide</a>, <a href="https://publications.waset.org/abstracts/search?q=whole%20genome%20sequencing%20analysis" title=" whole genome sequencing analysis"> whole genome sequencing analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=multi%20-drug%20resistance%20gram%20negative%20bacteria" title=" multi -drug resistance gram negative bacteria"> multi -drug resistance gram negative bacteria</a> </p> <a href="https://publications.waset.org/abstracts/164251/identification-and-characterization-of-antimicrobial-peptides-isolated-from-entophytic-bacteria-and-their-activity-against-multidrug-resistance-gram-negative-bacteria-in-south-korea" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/164251.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">81</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1545</span> Investigation of Clubroot Disease Occurrence under Chemical and Organic Soil Environment</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zakirul%20Islam">Zakirul Islam</a>, <a href="https://publications.waset.org/abstracts/search?q=Yugo%20Kumokawa"> Yugo Kumokawa</a>, <a href="https://publications.waset.org/abstracts/search?q=Quoc%20Thinh%20Tran"> Quoc Thinh Tran</a>, <a href="https://publications.waset.org/abstracts/search?q=Motoki%20Kubo"> Motoki Kubo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Clubroot is a disease of cruciferous plant caused by soil born pathogen Plasmodiophora brassicae and can significantly limit the production through rapid spreading. The present study was designed to investigate the effect of cultivation practices (chemical and organic soils) on clubroot disease development in Brassica rapa. Disease index and root bacterial composition were investigated for both chemical and organic soils. The bacterial biomass and diversity in organic soil were higher than those in chemical soil. Disease severity was distinct for two different cultivation methods. The number of endophytic bacteria decreased in the infected root for both soils. The increased number of endophytic bacterial number led to reduce the proliferation of pathogen spore inside the root and thus reduced the disease severity in organic plants. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=clubroot%20disease" title="clubroot disease">clubroot disease</a>, <a href="https://publications.waset.org/abstracts/search?q=bacterial%20biomass" title=" bacterial biomass"> bacterial biomass</a>, <a href="https://publications.waset.org/abstracts/search?q=root%20infection" title=" root infection"> root infection</a>, <a href="https://publications.waset.org/abstracts/search?q=disease%20index" title=" disease index"> disease index</a>, <a href="https://publications.waset.org/abstracts/search?q=chemical%20cultivation" title=" chemical cultivation"> chemical cultivation</a>, <a href="https://publications.waset.org/abstracts/search?q=organic%20cultivation" title=" organic cultivation"> organic cultivation</a> </p> <a href="https://publications.waset.org/abstracts/169607/investigation-of-clubroot-disease-occurrence-under-chemical-and-organic-soil-environment" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/169607.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">90</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1544</span> Seed Associated Microbial Communities of Holoparasitic Cistanche Species from Armenia and Portugal</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=K.%20Petrosyan">K. Petrosyan</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20Piwowarczyk"> R. Piwowarczyk</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20Rura%C5%BC"> K. Ruraż</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Thijs"> S. Thijs</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Vangronsveld"> J. Vangronsveld</a>, <a href="https://publications.waset.org/abstracts/search?q=W.%20Kaca"> W. Kaca</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Holoparasitic plants are flowering heterotrophic angiosperms which with the help of an absorbing organ - haustorium, attach to another plant, the so-called the host. Due to the different hosts, unusual lifestyle, lack of roots, chlorophylls and photosynthesis, these plants are interesting and unique study objects for global biodiversity. The seeds germination of the parasitic plants also is unique: they germinate only in response to germination stimulants, namely strigolactones produced by the root of an appropriate host. Resistance of the seeds on different environmental conditions allow them to stay viable in the soil for more than 20 years. Among the wide range of plant protection mechanisms the endophytic communities have a specific role. In this way, they have the potential to mitigate the impacts of adverse conditions such as soil salinization. The major objective of our study was to compare the bacterial endo-microbiomes from seeds of two holoparasitic plants from Orobanchaceae family, Cistanche – C. armena (Armenia) and C. phelypaea (Portugal) – from saline habitats different in soil water status. The research aimed to perform how environmental conditions influence on the diversity of the bacterial communities of C. armena and C. phelypaea seeds. This was achieved by comparison of the endophytic microbiomes of two species and isolation of culturable bacteria. A combination of culture-dependent and molecular techniques was employed for the identification of the seed endomicrobiome (culturable and unculturable). Using the V3-V4 hypervariable region of the 16S rRNA gene, four main taxa were identified: Proteobacteria, Actinobacteria, Bacteroidetes, Firmicutes, but the relative proportion of the taxa was different in each type of seed. Generally, sixteen phyla, 323 genera and 710 bacterial species were identified, mainly Gram negative, halotolerant bacteria with an environmental origin. However, also some unclassified and unexplored taxonomic groups were found in the seeds of both plants. 16S rRNA gene sequencing analysis from both species identified the gram positive, endospore forming, halotolerant and alkaliphile Bacillus spp. which suggests that the endophytic bacteria of examined seeds possess traits that are correlated with the natural habitat of their hosts. The cultivable seed endophytes from C. armena and C. phelypaea were rather similar, notwithstanding the big distances between their growth habitats - Armenia and Portugal. Although the seed endophytic microbiomes of C. armena and C. phelypaea contain a high number of common bacterial taxa, also remarkable differences exist. We demonstrated that the environmental conditions or abiotic stresses influence on diversity of the bacterial communities of holoparasiotic seeds. To the best of our knowledge the research is the first report of endophytes from seeds of holoparasitic Cistanche armena and C. phelypaea plants. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=microbiome" title="microbiome">microbiome</a>, <a href="https://publications.waset.org/abstracts/search?q=parasitic%20plant" title=" parasitic plant"> parasitic plant</a>, <a href="https://publications.waset.org/abstracts/search?q=salinity" title=" salinity"> salinity</a>, <a href="https://publications.waset.org/abstracts/search?q=seeds" title=" seeds"> seeds</a> </p> <a href="https://publications.waset.org/abstracts/144216/seed-associated-microbial-communities-of-holoparasitic-cistanche-species-from-armenia-and-portugal" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/144216.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">75</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1543</span> Biotransformation of Monoterpenes by Whole Cells of Eleven Praxelis clematidea-Derived Endophytic Fungi</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Daomao%20Yang">Daomao Yang</a>, <a href="https://publications.waset.org/abstracts/search?q=Qizhi%20Wang"> Qizhi Wang</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Monoterpenoids are mainly found in plant essential oils and they are ideal substrates for biotransformation into oxygen-containing derivatives with important commercial value due to their low price and simple structure. In this paper, eleven strains of endophytic fungi from <em>Praxelis clematidea</em> were used as test strains to conduct the whole cell biotransformation of the monoterpenoids: (+)-limonene, (-)-limonene and myrcene. The fungi were inoculated in 50 ml Sabouraud medium and incubated at 30 ℃ with the agitation of 150 r/min for 6 d, and then 0.5% (v/v) substrates were added into the medium and biotransformed for further 3 d. Afterwards the cultures were filtered, and extracted using equal volume of ethyl acetate. The metabolites were analyzed by GC-MS technique with NIST database. The Total Ion Chromatogram of the extractions from the eleven strains showed that the main product of (+)- and (-)-limonene biotransformation was limonene-1,2-diol, while it is limonene and linalool oxide for biotransformation of myrcene. This work will help screen the microorganisms to biotransform the monoterpenes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=endophytic%20fungi" title="endophytic fungi">endophytic fungi</a>, <a href="https://publications.waset.org/abstracts/search?q=%28%2B%29%E2%80%93limonene" title=" (+)–limonene"> (+)–limonene</a>, <a href="https://publications.waset.org/abstracts/search?q=%28-%29%E2%80%93limonene" title=" (-)–limonene"> (-)–limonene</a>, <a href="https://publications.waset.org/abstracts/search?q=myrcene" title=" myrcene"> myrcene</a> </p> <a href="https://publications.waset.org/abstracts/132414/biotransformation-of-monoterpenes-by-whole-cells-of-eleven-praxelis-clematidea-derived-endophytic-fungi" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/132414.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">131</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1542</span> Selection Effects on the Molecular and Abiotic Evolution of Antibiotic Resistance</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Abishek%20Rajkumar">Abishek Rajkumar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Antibiotic resistance can occur naturally given the selective pressure placed on antibiotics. Within a large population of bacteria, there is a significant chance that some of those bacteria can develop resistance via mutations or genetic recombination. However, a growing public health concern has arisen over the fact that antibiotic resistance has increased significantly over the past few decades. This is because humans have been over-consuming and producing antibiotics, which has ultimately accelerated the antibiotic resistance seen in these bacteria. The product of all of this is an ongoing race between scientists and the bacteria as bacteria continue to develop resistance, which creates even more demand for an antibiotic that can still terminate the newly resistant strain of bacteria. This paper will focus on a myriad of aspects of antibiotic resistance in bacteria starting with how it occurs on a molecular level and then focusing on the antibiotic concentrations and how they affect the resistance and fitness seen in bacteria. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antibiotic" title="antibiotic">antibiotic</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular" title=" molecular"> molecular</a>, <a href="https://publications.waset.org/abstracts/search?q=mutation" title=" mutation"> mutation</a>, <a href="https://publications.waset.org/abstracts/search?q=resistance" title=" resistance"> resistance</a> </p> <a href="https://publications.waset.org/abstracts/66066/selection-effects-on-the-molecular-and-abiotic-evolution-of-antibiotic-resistance" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/66066.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">330</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1541</span> The Existence of Beauveria bassiana in the Third Generation of Corn Seedling</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Itji%20Diana%20Daud">Itji Diana Daud</a>, <a href="https://publications.waset.org/abstracts/search?q=Nuniek%20Widiayani"> Nuniek Widiayani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The fungus Beauveria bassiana can be endophytic in maize. The fungus was recovered in culture from stems, leaves and roots after a month planting. This phenomenon was shown until the third generation of the corn. The result from laboratory shows that B. bassiana appear in F1, F2 and F3 in order 70, 80 and 90% in the roots, 80% in the stems in all generation, 90, 80 and 70% in leaves. In CFU’s ml-1 of B. bassiana in corn seed, show F1 was 8.9 x 106, F2 was 8.1 x 106 and F3 was 7.8 x 106. The research showed that B. Bassiana as endophyte still remain to the third generation. Innovation to the corn seed which is endophyte seed is essential to protect from the attack of corn borer and to avoid the usage of insecticide. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=endophytic" title="endophytic">endophytic</a>, <a href="https://publications.waset.org/abstracts/search?q=recovered" title=" recovered"> recovered</a>, <a href="https://publications.waset.org/abstracts/search?q=third%20generation" title=" third generation"> third generation</a>, <a href="https://publications.waset.org/abstracts/search?q=Beauveria%20bassiana" title=" Beauveria bassiana"> Beauveria bassiana</a> </p> <a href="https://publications.waset.org/abstracts/41128/the-existence-of-beauveria-bassiana-in-the-third-generation-of-corn-seedling" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/41128.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">291</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1540</span> Effect of Biostimulants on Downstream Processing of Endophytic Fungi Hosted in Aromatic Plant, Ocimum basicilium</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kanika%20Chowdhary">Kanika Chowdhary</a>, <a href="https://publications.waset.org/abstracts/search?q=Satyawati%20Sharma"> Satyawati Sharma</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Endophytic microbes are hosted inside plants in a symbiotic and hugely benefitting relationship. Exploring agriculturally beneficial endophytes is quite a prospective field of research. In the present work fungal endophytes associated with aromatic plant Ocimum basicilium L. were investigated for biocontrol potential. The anti-plant pathogenic activity of fungal endophytes was tested against causal agent of stem rot Sclerotinia sclerotiorum. 75 endophytic fungi were recovered through culture-dependent approach. Fungal identification was performed both microscopically and by rDNA ITS sequencing. Curvuaria lunata (Sb-6) and Colletotrichum lindemuthianum (Sb-8) inhibited 86% and 72% mycelia growth of S. sclerotinia on Sabouraud dextrose agar medium at 7.4 pH. Small-scale fermentation was carried out on sterilised oatmeal grain medium. In another set of experiment, fungi were grown in oatmeal grain medium amended with certain biostimulants such as aqueous seaweed extract (10% v/w); methanolic seaweed extract (5% v/w); cow urine (20% v/w); biochar (10% w/w) in triplicate along with control of each to ascertain the degree of metabolic difference and anti-plant pathogenic activity induced. Phytochemically extracts of both the fungal isolates showed the presence of flavanoids, phenols, tannins, alkaloids and terpenoids. Ethylacetate extract of C. lunata and C. lindemuthianum suppressed S. sclerotinia conidial germination at IC50 values of 0.514± 0.02 and 0.913± 0.04 mg/ml. Therefore, fungal endophytes of O. basicilium are highly promising bio-resource agent, which can be developed further for sustainable agriculture. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=endophytic%20fungi" title="endophytic fungi">endophytic fungi</a>, <a href="https://publications.waset.org/abstracts/search?q=ocimum%20basicilium" title=" ocimum basicilium"> ocimum basicilium</a>, <a href="https://publications.waset.org/abstracts/search?q=sclerotinia%20sclerotiorum" title=" sclerotinia sclerotiorum"> sclerotinia sclerotiorum</a>, <a href="https://publications.waset.org/abstracts/search?q=biostimulants" title=" biostimulants"> biostimulants</a> </p> <a href="https://publications.waset.org/abstracts/86687/effect-of-biostimulants-on-downstream-processing-of-endophytic-fungi-hosted-in-aromatic-plant-ocimum-basicilium" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/86687.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">183</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1539</span> Evaluation of Antimicrobial Activity of Different Dithiolethiones</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zehour%20Rahmani">Zehour Rahmani</a>, <a href="https://publications.waset.org/abstracts/search?q=Messouda%20Dekmouche"> Messouda Dekmouche</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20Hadjadj"> Mohamed Hadjadj</a>, <a href="https://publications.waset.org/abstracts/search?q=Mokhtar%20Saidi"> Mokhtar Saidi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the last decades of the nineteenth century, the study of disease – causing microorganisms became concentrated on bacteria and largely institutionalized. In earlier years, the scientists interested in bacteria had originally been chemists like Pasteur, physicists like Tyndall, or botanists like Cohn and ward. For this reason, the objective of this research was to evaluate the potential of some dithiolethiones on standard microorganism strains as well as multi-drug resistant bacteria, which were isolated from hospitals. Recent studies have demonstrated, that several dithiolethione compounds, particularly (3H-1,2-dithiole-3-thione), exhibit the biological activities against several bacteria. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bacteria" title="bacteria">bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=dithiolethiones" title=" dithiolethiones"> dithiolethiones</a>, <a href="https://publications.waset.org/abstracts/search?q=microorganism" title=" microorganism"> microorganism</a>, <a href="https://publications.waset.org/abstracts/search?q=potential" title=" potential "> potential </a> </p> <a href="https://publications.waset.org/abstracts/39624/evaluation-of-antimicrobial-activity-of-different-dithiolethiones" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/39624.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">329</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1538</span> Lifestyle Switching Phenomenon of Plant Associated Fungi</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gauravi%20Agarkar">Gauravi Agarkar</a>, <a href="https://publications.waset.org/abstracts/search?q=Mahendra%20Rai"> Mahendra Rai</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Fungi are closely associated with the plants in various types of interactions such as mycorrhizal, parasitic or endophytic. Some of these interactions are beneficial and a few are harmful to the host plants. It has been suggested that these plant-associated fungi are able to change their lifestyle abd this means endophyte may become parasite or vice versa. This phenomenon may have profound effect on plant-fungal interactions and various ecological niches. Therefore, it is necessary to identify the factors that trigger the change in fungal lifestyle and understand whether these different lifestyles are interconnected at some points either by physiological, biochemical or molecular routes. This review summarizes the factors affecting plant fungal interactions and discusses the possible mechanisms for lifestyles switching of fungi based on available experimental evidences. Research should be boosted in this direction to fetch more advantages in future and to avoid the severe consequences in agriculture and other related fields. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=endophytic" title="endophytic">endophytic</a>, <a href="https://publications.waset.org/abstracts/search?q=lifestyle%20switching" title=" lifestyle switching"> lifestyle switching</a>, <a href="https://publications.waset.org/abstracts/search?q=mycorrhizal" title=" mycorrhizal"> mycorrhizal</a>, <a href="https://publications.waset.org/abstracts/search?q=parasitic" title=" parasitic"> parasitic</a>, <a href="https://publications.waset.org/abstracts/search?q=plant-fungal%20interactions" title=" plant-fungal interactions"> plant-fungal interactions</a> </p> <a href="https://publications.waset.org/abstracts/23774/lifestyle-switching-phenomenon-of-plant-associated-fungi" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/23774.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">423</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1537</span> Setting the Acceleration Test Conditions for Establishing the Expiration Date of Probiotics</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Myoyeon%20Kim">Myoyeon Kim</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The number of probiotics is various from product to product. The product must contain as many bacteria as the number of bacteria that claim because it greatly affects consumers' choices. It is very difficult to determine the number of viable bacteria with tests that proceed during the product development stage because the shelf life of lactic acid bacteria is mostly 18 to 24 months, and product development proceeds much faster than this. To predict the shelf life, a method of checking the number of viable bacteria was studied by shortening the time. The experiment was conducted with a total of 7 products including our products. The ongoing test stored at room temperature, the acceleration test stored at 30°C and 40°C were performed, and the number of bacteria was measured every two weeks. The number of viable bacteria stored at 30°C for 12 weeks was similar to the ongoing test when the shelf life was imminent. If it took more than 12 weeks, the product development schedule was postponed, so acceleration had no meaning. It was found that products stored at 40°C were unsuitable as acceleration test temperatures because the bacteria were almost killed within 4 to 8 weeks. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=probiotics" title="probiotics">probiotics</a>, <a href="https://publications.waset.org/abstracts/search?q=shelf-life" title=" shelf-life"> shelf-life</a>, <a href="https://publications.waset.org/abstracts/search?q=acceleration%20test" title=" acceleration test"> acceleration test</a>, <a href="https://publications.waset.org/abstracts/search?q=lactobacillus" title=" lactobacillus"> lactobacillus</a> </p> <a href="https://publications.waset.org/abstracts/188379/setting-the-acceleration-test-conditions-for-establishing-the-expiration-date-of-probiotics" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/188379.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">45</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1536</span> Effects of Bacteria on Levels of AFM1 in Phosphate Buffer at Different Level of Energy Source</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ali%20M.%20Elgerbi">Ali M. Elgerbi</a>, <a href="https://publications.waset.org/abstracts/search?q=Obied%20A.%20Alwan"> Obied A. Alwan</a>, <a href="https://publications.waset.org/abstracts/search?q=Al-Taher%20O.%20Alzwei"> Al-Taher O. Alzwei</a>, <a href="https://publications.waset.org/abstracts/search?q=Abdurrahim%20A.%20Elouzi"> Abdurrahim A. Elouzi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The binding of AFM1 to bacteria in phosphate buffer solution depended on many factors such as: availability of energy, incubation period, species and strain of bacteria. Increase in concentration of sugar showed higher removal of AFM1 and faster than in phosphate buffer alone. With 1.0% glucose lactic acid bacteria and bifidobacteria showed toxin removal ranging from 7.7 to 39.7% whereas with 10.0% glucose the percentage removal was 21.8 to 45.4% at 96 hours of incubation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=aflatoxin%20M1" title="aflatoxin M1">aflatoxin M1</a>, <a href="https://publications.waset.org/abstracts/search?q=lactic%20acid%20bacteria" title=" lactic acid bacteria"> lactic acid bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=bifidobacteria" title=" bifidobacteria "> bifidobacteria </a>, <a href="https://publications.waset.org/abstracts/search?q=binding" title=" binding"> binding</a>, <a href="https://publications.waset.org/abstracts/search?q=phosphate%20buffer" title=" phosphate buffer "> phosphate buffer </a> </p> <a href="https://publications.waset.org/abstracts/19875/effects-of-bacteria-on-levels-of-afm1-in-phosphate-buffer-at-different-level-of-energy-source" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/19875.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">511</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1535</span> A Pink-Pigmented Facultative Methylobacterium sp Isolated from Retama monosperma Root Nodules</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=N.%20Selami">N. Selami</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Kaid%20Harche"> M. Kaid Harche </a> </p> <p class="card-text"><strong>Abstract:</strong></p> A pink-pigmented, aerobic, facultatively methylotrophic bacterium, was isolated from Retama monosperma root nodules and identified as a member of the genus Methylobacterium. Inoculation of R. monosperma plants by a pure culture of isolate strain under a hydroponic condition, resulted, 10 dpi, the puffiness at lateral roots. The observation in detail the anatomy and ultra-structure of infection sites by light and electron microscopy show that the bacteria induce stimulation of the division of cortical cells and digestion of epidermis cells then, Methylobacterium was observed in the inter and intracellular spaces of the outer cortex root. These preliminary results allow us to suggest the establishment of an epi-endosymbiotic interaction between Methylobacterium sp and R. monosperma. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=endophytic%20colonization" title="endophytic colonization">endophytic colonization</a>, <a href="https://publications.waset.org/abstracts/search?q=Methylobacterium" title=" Methylobacterium"> Methylobacterium</a>, <a href="https://publications.waset.org/abstracts/search?q=microscopy" title=" microscopy"> microscopy</a>, <a href="https://publications.waset.org/abstracts/search?q=nodule" title=" nodule"> nodule</a>, <a href="https://publications.waset.org/abstracts/search?q=pink%20pigmented" title=" pink pigmented"> pink pigmented</a>, <a href="https://publications.waset.org/abstracts/search?q=Retama%20monosperma" title=" Retama monosperma "> Retama monosperma </a> </p> <a href="https://publications.waset.org/abstracts/22282/a-pink-pigmented-facultative-methylobacterium-sp-isolated-from-retama-monosperma-root-nodules" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/22282.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">368</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1534</span> Bacteria Removal from Wastewater by Electrocoagulation Process</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Boudjema%20Nouara">Boudjema Nouara</a>, <a href="https://publications.waset.org/abstracts/search?q=Mameri%20%20Nabil"> Mameri Nabil</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Bacteria have played an important role in water contamination as a consequence of organic pollution. In this study, an electrocoagulation process was adopted to remove fecal contamination and pathogenic bacteria from waste water. The effect of anode/cathodes materials as well as operating conditions for bacteria removal from water, such as current intensity and initial pH and temperature. The results indicated that the complete removal was achevied when using aluminium anode as anode at current intensity of 3A, initial pH of 7-8 and electrolysis time of 30 minutes. This process showed a bactericidal effect of 95 to 99% for the total and fecal coliforms and 99% to 100% for Eschercichia coli and fecal Streptococci. A decrease of 72% was recorded for sulphite-reducing Clostridia. Thus, this process has the potential to be one the options for treatment where high amount of bacteria in wastewater river. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bacteria" title="bacteria">bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=el%20Harrach%20river" title=" el Harrach river"> el Harrach river</a>, <a href="https://publications.waset.org/abstracts/search?q=electrocoagulation" title=" electrocoagulation"> electrocoagulation</a>, <a href="https://publications.waset.org/abstracts/search?q=wastewater" title=" wastewater"> wastewater</a>, <a href="https://publications.waset.org/abstracts/search?q=treatment" title=" treatment"> treatment</a> </p> <a href="https://publications.waset.org/abstracts/28065/bacteria-removal-from-wastewater-by-electrocoagulation-process" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/28065.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">500</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1533</span> Isolation and Characterization of Indigenous Rhizosphere Bacteria Producing Gibberellin Acid from Local Soybeans in Three Different Areas of South Sulawesi</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Asmiaty%20Sahur">Asmiaty Sahur</a>, <a href="https://publications.waset.org/abstracts/search?q=Ambo%20Ala"> Ambo Ala</a>, <a href="https://publications.waset.org/abstracts/search?q=Baharuddin%20Patanjengi"> Baharuddin Patanjengi</a>, <a href="https://publications.waset.org/abstracts/search?q=Elkawakib%20Syam%27un"> Elkawakib Syam'un</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study aimed to isolate and characterize the indigenous Rhizosphere bacteria producing Gibberellin Acid as plant growth isolated from local soybean of three different areas in South Sulawesi, Indonesia. Several soil samples of soybean plants were collected from the Rhizosphere of local soybeans in three different areas of South Sulawesi such as Soppeng, Bone and Takalar. There were 56 isolates of bacteria were isolated and grouped into gram-positive bacteria and gram negative bacteria .There are 35 isolates produce a thick slime or slimy when cultured on media Natrium Broth and the remaining of those produced spores. The results showed that of potential bacterial isolated produced Gibberellin Acid in high concentration. The best isolate of Rhizosphere bacteria for the production of Gibberellin Acid is with concentration 2%. There are 4 isolates that had higher concentration are AKB 19 (4.67 mg/ml) followed by RKS 17 (3.80 mg/ml), RKS 25 (3.70 mg / ml) and RKS 24 (3.29 mg/ml) respectively. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=rhizosphere" title="rhizosphere">rhizosphere</a>, <a href="https://publications.waset.org/abstracts/search?q=bacteria" title=" bacteria"> bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=gibberellin%20acid" title=" gibberellin acid"> gibberellin acid</a>, <a href="https://publications.waset.org/abstracts/search?q=soybeans" title=" soybeans"> soybeans</a> </p> <a href="https://publications.waset.org/abstracts/35624/isolation-and-characterization-of-indigenous-rhizosphere-bacteria-producing-gibberellin-acid-from-local-soybeans-in-three-different-areas-of-south-sulawesi" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/35624.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">240</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1532</span> Endophytic Fungi Recovered from Lycium arabicum as an Eco-Friendly Alternative for Fusarium Crown and Root Rot Disease Control and Tomato Growth Enhancement</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ahlem%20Nefzi">Ahlem Nefzi</a>, <a href="https://publications.waset.org/abstracts/search?q=Rania%20Aydi%20Ben%20Abdallah"> Rania Aydi Ben Abdallah</a>, <a href="https://publications.waset.org/abstracts/search?q=Hayfa%20Jabnoun-Khiareddine"> Hayfa Jabnoun-Khiareddine</a>, <a href="https://publications.waset.org/abstracts/search?q=Ammar%20Nawaim"> Ammar Nawaim</a>, <a href="https://publications.waset.org/abstracts/search?q=Rabiaa%20Haouala"> Rabiaa Haouala</a>, <a href="https://publications.waset.org/abstracts/search?q=Mejda%20Daami-Remadi"> Mejda Daami-Remadi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Seven endophytic fungi were isolated from the wild Solanaceous species Lycium arabicum growing in the Tunisian Centre-East and were assessed for their ability to suppress Fusarium Crown and Root Rot disease caused by Fusarium oxysporum f. sp. radicis lycopersici (FORL) and to enhance plant growth. Fungal isolates were shown able to colonize tomato cv. Rio Grande roots, crowns, and stems. A significant promotion in all studied growth parameters (root length, shoot height, and roots and shoots fresh weight) was recorded in tomato plants treated with fungal conidial suspensions or their cell-free culture filtrates compared to FORL-inoculated or pathogen-free controls. I15 and I18 isolates were shown to be the most effective leading to 85.7-87.5 and 93.6-98.4% decrease in leaf and root damage index and the vascular discoloration extent, respectively, over FORL-inoculated and untreated control. These two bioactive and growth-promoting isolates (I15 and I18) were morphologically characterized and identified using rDNA sequencing gene as being Alternaria alternata (MF693801) and Fusarium fujikuroi (MF693802). These fungi significantly suppressed FORL mycelial growth and showed chitinolytic, proteolytic and amylase activities whereas only F. fujikuroi displayed a lipolytic activity. This study clearly demonstrated the potential use of fungi naturally associated with L. arabicum as biocontrol and bio-fertilizing agents. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biocontrol" title="biocontrol">biocontrol</a>, <a href="https://publications.waset.org/abstracts/search?q=endophytic%20fungi" title=" endophytic fungi"> endophytic fungi</a>, <a href="https://publications.waset.org/abstracts/search?q=Fusarium%20oxysporum%20f.%20sp.%20radicis-lycopersici" title=" Fusarium oxysporum f. sp. radicis-lycopersici"> Fusarium oxysporum f. sp. radicis-lycopersici</a>, <a href="https://publications.waset.org/abstracts/search?q=tomato%20promotion" title=" tomato promotion"> tomato promotion</a>, <a href="https://publications.waset.org/abstracts/search?q=Lycium%20arabicum" title=" Lycium arabicum"> Lycium arabicum</a> </p> <a href="https://publications.waset.org/abstracts/90549/endophytic-fungi-recovered-from-lycium-arabicum-as-an-eco-friendly-alternative-for-fusarium-crown-and-root-rot-disease-control-and-tomato-growth-enhancement" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/90549.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">177</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1531</span> The Effect of Bacteria on Mercury's Biological Removal</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nastaran%20Soltani">Nastaran Soltani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Heavy metals such as Mercury are toxic elements that enter the environment through different ways and endanger the environment, plants, animals, and humans’ health. Microbial activities reduce the amount of heavy metals. Therefore, an effective mechanism to eliminate heavy metals in the nature and factory slops, is using bacteria living in polluted areas. Karun River in Khuzestan Province in Iran has been always polluted by heavy metals as it is located among different industries in the region. This study was performed based on the data from sampling water and sediments of four stations across the river during the four seasons of a year. The isolation of resistant bacteria was performed through enrichment and direct cultivation in a solid medium containing mercury. Various bacteria such as Pseudomonas sp., Serratia Marcescens, and E.coli were identified as mercury-resistant bacteria. The power of these bacteria to remove mercury varied from 28% to 86%, with strongest power belonging to Pseudomonas sp. isolated in spring making a good candidate to be used for mercury biological removal from factory slops. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bacteria" title="bacteria">bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=Karun%20River" title=" Karun River"> Karun River</a>, <a href="https://publications.waset.org/abstracts/search?q=mercury" title=" mercury"> mercury</a>, <a href="https://publications.waset.org/abstracts/search?q=biological%20removal" title=" biological removal"> biological removal</a>, <a href="https://publications.waset.org/abstracts/search?q=mercury-resistant" title=" mercury-resistant"> mercury-resistant</a> </p> <a href="https://publications.waset.org/abstracts/46736/the-effect-of-bacteria-on-mercurys-biological-removal" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/46736.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">291</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1530</span> Examining the Presence of Heterotrophic Aerobic Bacteria (HAB), and Sulphate Reducing Bacteria (SRB) in Some Types of Water from the City of Tripoli, Libya</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Abdulsalam.%20I.%20Rafida">Abdulsalam. I. Rafida</a>, <a href="https://publications.waset.org/abstracts/search?q=Marwa.%20F.%20Elalem"> Marwa. F. Elalem</a>, <a href="https://publications.waset.org/abstracts/search?q=Hasna.%20E.%20Alemam"> Hasna. E. Alemam</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study aimed at testing the various types of water in some areas of the city of Tripoli, Libya for the presence of Heterotrophic Aerobic Bacteria (HAB), and anaerobic Sulphate Reducing Bacteria (SRB). The water samples under investigation included rainwater accumulating on the ground, sewage water (from the city sewage treatment station, sulphate water from natural therapy swimming sites), and sea water (i.e. sea water exposed to pollution by untreated sewage water, and unpolluted sea water from specific locations). A total of 20 samples have been collected distributed as follows: rain water (8 samples), sewage water (6 samples), and sea water (6 samples). An up-to-date method for estimation has been used featuring readymade solutions i.e. (BARTTM test for HAB and BARTTM test for SRB). However, with the exception of one rain water sample, the results have indicated that the target bacteria have been present in all samples. Regarding HAB bacteria the samples have shown a maximum average of 7.0 x 106 cfu/ml featuring sewage and rain water and a minimum average of 1.8 x 104 cuf/ml featuring unpolluted sea water collected from a specific location. As for SRB bacteria; a maximum average of 7.0 x 105 cfu/ml has been shown by sewage and rain water and a minimum average of 1.8 x 104 cfu/ml by sewage and sea water. The above results highlight the relationship between pollution and the presence of bacteria in water particularly water collected from specific locations, and also the presence of bacteria as the result of the use of water provided that a suitable environment exists for its growth. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=heterotrophic%20aerobic%20bacteria%20%28HAB%29" title="heterotrophic aerobic bacteria (HAB)">heterotrophic aerobic bacteria (HAB)</a>, <a href="https://publications.waset.org/abstracts/search?q=sulphate%20reducing%20bacteria%20%28SRB%29" title=" sulphate reducing bacteria (SRB)"> sulphate reducing bacteria (SRB)</a>, <a href="https://publications.waset.org/abstracts/search?q=water" title=" water"> water</a>, <a href="https://publications.waset.org/abstracts/search?q=environmental%20sciences" title=" environmental sciences"> environmental sciences</a> </p> <a href="https://publications.waset.org/abstracts/19642/examining-the-presence-of-heterotrophic-aerobic-bacteria-hab-and-sulphate-reducing-bacteria-srb-in-some-types-of-water-from-the-city-of-tripoli-libya" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/19642.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">495</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=endophytic%20bacteria&page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=endophytic%20bacteria&page=3">3</a></li> <li class="page-item"><a class="page-link" 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class="page-link">...</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=endophytic%20bacteria&page=51">51</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=endophytic%20bacteria&page=52">52</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=endophytic%20bacteria&page=2" rel="next">&rsaquo;</a></li> </ul> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th 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