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href="https://sites.uw.edu/biochemcore/" title="Hans Neurath Biophysics Core">Hans Neurath Biophysics Core</a><li></ul></nav> <div id='main_content' class="uw-body-copy" tabindex="-1"> <h1>Instruments</h1> <p>The Hans Neurath Biophysics Core is home to numerous core instruments available for use by members of the UW Department of Biochemistry. To gain training and access to these instruments, contact <a href="mailto:biochemcore@uw.edu">Lisa Tuttle</a> with a description of you project and instrument needs.</p> <table style="border-collapse: collapse; width: 100%; height: 1276px;"> <tbody> <tr style="height: 162px;"> <td style="width: 20.3581%; height: 162px;"><u><a href="https://sites.uw.edu/biochemcore/files/2023/02/20230210_111313.jpg"><img fetchpriority="high" decoding="async" class="alignleft wp-image-180 size-medium" src="https://sites.uw.edu/biochemcore/files/2023/02/20230210_111313-300x300.jpg" alt="Circular Dichroism Instrument" width="300" height="300" srcset="https://sites.uw.edu/biochemcore/files/2023/02/20230210_111313-300x300.jpg 300w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_111313-1024x1024.jpg 1024w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_111313-150x150.jpg 150w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_111313-768x768.jpg 768w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_111313-1536x1536.jpg 1536w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_111313-200x200.jpg 200w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_111313-50x50.jpg 50w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_111313-375x375.jpg 375w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_111313-750x750.jpg 750w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_111313-1140x1140.jpg 1140w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_111313.jpg 1920w" sizes="(max-width: 300px) 100vw, 300px" /></a></u></td> <td style="width: 79.6419%; height: 162px;"><u>Circular Dichroism.</u> Jasco J-1500 circular dichroism spectrometer, capable of high sensitivity and resolution linear dichroism and absorbance, with measurable wavelengths ranging from 163 nm to 950 nm. The instrument is also equipped with a Peltier cell holder for accurate and rapid temperature control.</p> <p><a href="https://jascoinc.com/products/spectroscopy/circular-dichroism/j-1000-series-models/j-1500-circular-dichroism-spectrophotometer/">https://jascoinc.com/products/spectroscopy/circular-dichroism/j-1000-series-models/j-1500-circular-dichroism-spectrophotometer/</a></td> </tr> <tr style="height: 316px;"> <td style="width: 20.3581%; height: 316px;"><u><a href="https://sites.uw.edu/biochemcore/files/2023/02/20230210_113355-300x300.jpg"><img decoding="async" class="alignleft wp-image-176 size-medium" src="https://sites.uw.edu/biochemcore/files/2023/02/20230210_113355-300x300.jpg" alt="Photograph of Mass Photometer" width="300" height="300" srcset="https://sites.uw.edu/biochemcore/files/2023/02/20230210_113355-300x300.jpg 300w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113355-1024x1024.jpg 1024w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113355-150x150.jpg 150w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113355-768x768.jpg 768w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113355-1536x1536.jpg 1536w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113355-200x200.jpg 200w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113355-50x50.jpg 50w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113355-375x375.jpg 375w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113355-750x750.jpg 750w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113355-1140x1140.jpg 1140w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113355.jpg 1920w" sizes="(max-width: 300px) 100vw, 300px" /></a></u></td> <td style="width: 79.6419%; height: 316px;"><u>Mass Photometer.</u> A ReFeyn Two-MP mass photometer is available for rapid determination of single-particle mass measurements in solution. This instrument is now also equipped with a <a href="https://refeyn.com/massfluidix-hc-system">Mass Fluidix</a> add-on that allows for rapid dilution of samples and subsequent MP measurement.</p> <p><a href="https://www.refeyn.com/twomp-mass-photometer">https://www.refeyn.com/twomp-mass-photometer</a></td> </tr> <tr> <td style="width: 20.3581%;"><a href="https://sites.uw.edu/biochemcore/files/2023/08/20230824_155529.jpg"><img decoding="async" class="alignleft wp-image-218 size-medium" src="https://sites.uw.edu/biochemcore/files/2023/08/20230824_155529-300x300.jpg" alt="Wyatt DLS" width="300" height="300" srcset="https://sites.uw.edu/biochemcore/files/2023/08/20230824_155529-300x300.jpg 300w, https://sites.uw.edu/biochemcore/files/2023/08/20230824_155529-1024x1024.jpg 1024w, https://sites.uw.edu/biochemcore/files/2023/08/20230824_155529-150x150.jpg 150w, https://sites.uw.edu/biochemcore/files/2023/08/20230824_155529-768x768.jpg 768w, https://sites.uw.edu/biochemcore/files/2023/08/20230824_155529-1536x1536.jpg 1536w, https://sites.uw.edu/biochemcore/files/2023/08/20230824_155529-200x200.jpg 200w, https://sites.uw.edu/biochemcore/files/2023/08/20230824_155529-50x50.jpg 50w, https://sites.uw.edu/biochemcore/files/2023/08/20230824_155529-375x375.jpg 375w, https://sites.uw.edu/biochemcore/files/2023/08/20230824_155529-750x750.jpg 750w, https://sites.uw.edu/biochemcore/files/2023/08/20230824_155529-1140x1140.jpg 1140w, https://sites.uw.edu/biochemcore/files/2023/08/20230824_155529.jpg 1920w" sizes="(max-width: 300px) 100vw, 300px" /></a></td> <td style="width: 79.6419%;"><u>Dynamic Light Scattering.</u> A Wyatt DLS is available for determining sizes of complexes in solution using a 96-well plate.</p> <p><a href="https://www.wyatt.com/solutions/techniques/dynamic-light-scattering-nanoparticle-size.html">https://www.wyatt.com/solutions/techniques/dynamic-light-scattering-nanoparticle-size.html</a></td> </tr> <tr style="height: 184px;"> <td style="width: 20.3581%; height: 184px;"><u><a href="https://sites.uw.edu/biochemcore/files/2023/02/20230210_111408.jpg"><img loading="lazy" decoding="async" class="alignleft wp-image-178 size-medium" src="https://sites.uw.edu/biochemcore/files/2023/02/20230210_111408-300x146.jpg" alt="Photograph of Fluorimeter" width="300" height="146" srcset="https://sites.uw.edu/biochemcore/files/2023/02/20230210_111408-300x146.jpg 300w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_111408-1024x498.jpg 1024w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_111408-768x373.jpg 768w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_111408-1536x746.jpg 1536w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_111408-375x182.jpg 375w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_111408-750x364.jpg 750w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_111408-1140x554.jpg 1140w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_111408.jpg 1920w" sizes="auto, (max-width: 300px) 100vw, 300px" /></a></u></td> <td style="width: 79.6419%; height: 184px;"><u>Fluorimeter.</u> A Fluorolog 3 (Horiba Scientific) fluorimeter with temperature control capability is available for FRET-SX measurements and other fluorescence spectra collection.</p> <p><a href="https://sites.uw.edu/biochemcore/files/2023/02/FluorEssence-v3.5-User-Manual-Part-Number-5500810000C.pdf">FluorEssence v3.5 User Manual</a><br /> <a href="https://sites.uw.edu/biochemcore/files/2023/02/Fluorolog-3-User-Manual-Part-Number-5500081014H.pdf">Fluorolog-3 User Manual </a></p> <p> </td> </tr> <tr style="height: 162px;"> <td style="width: 20.3581%; height: 162px;"><u><a href="https://sites.uw.edu/biochemcore/files/2023/02/20230210_113243.jpg"><img loading="lazy" decoding="async" class="alignleft wp-image-177 size-medium" src="https://sites.uw.edu/biochemcore/files/2023/02/20230210_113243-300x146.jpg" alt="Photograph of Octet R8" width="300" height="146" srcset="https://sites.uw.edu/biochemcore/files/2023/02/20230210_113243-300x146.jpg 300w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113243-1024x498.jpg 1024w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113243-768x373.jpg 768w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113243-1536x746.jpg 1536w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113243-375x182.jpg 375w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113243-750x364.jpg 750w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113243-1140x554.jpg 1140w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113243.jpg 1920w" sizes="auto, (max-width: 300px) 100vw, 300px" /></a></u></td> <td style="width: 79.6419%; height: 162px;"><u>Protein Analysis System.</u> A Sartorius Octet R8 is available for protein quantitation and kinetic analysis in a 96-well plate.</p> <p><a href="https://www.sartorius.com/en/products/protein-analysis/octet-bli-detection/octet-label-free-detection-systems/octet-r8">Octet庐 R8 Protein Analysis System | Sartorius</a></td> </tr> <tr> <td style="width: 20.3581%;"><a href="https://sites.uw.edu/biochemcore/files/2023/05/20230526_150452.jpg"><img loading="lazy" decoding="async" class="alignleft wp-image-214 size-medium" src="https://sites.uw.edu/biochemcore/files/2023/05/20230526_150452-300x300.jpg" alt="Affinity ITC" width="300" height="300" srcset="https://sites.uw.edu/biochemcore/files/2023/05/20230526_150452-300x300.jpg 300w, https://sites.uw.edu/biochemcore/files/2023/05/20230526_150452-1024x1024.jpg 1024w, https://sites.uw.edu/biochemcore/files/2023/05/20230526_150452-150x150.jpg 150w, https://sites.uw.edu/biochemcore/files/2023/05/20230526_150452-768x768.jpg 768w, https://sites.uw.edu/biochemcore/files/2023/05/20230526_150452-1536x1536.jpg 1536w, https://sites.uw.edu/biochemcore/files/2023/05/20230526_150452-200x200.jpg 200w, https://sites.uw.edu/biochemcore/files/2023/05/20230526_150452-50x50.jpg 50w, https://sites.uw.edu/biochemcore/files/2023/05/20230526_150452-375x375.jpg 375w, https://sites.uw.edu/biochemcore/files/2023/05/20230526_150452-750x750.jpg 750w, https://sites.uw.edu/biochemcore/files/2023/05/20230526_150452-1140x1140.jpg 1140w, https://sites.uw.edu/biochemcore/files/2023/05/20230526_150452.jpg 1920w" sizes="auto, (max-width: 300px) 100vw, 300px" /></a></td> <td style="width: 79.6419%;"><u>Affinity ITC.</u> Easily measure thermodynamic properties and Kds.</p> <p><a href="https://www.tainstruments.com/affinity-itc-auto/">https://www.tainstruments.com/affinity-itc-auto/</a></p> <p> </td> </tr> <tr> <td style="width: 20.3581%;"><u><a href="https://sites.uw.edu/biochemcore/files/2023/02/20230210_113442.jpg"><img loading="lazy" decoding="async" class="alignleft wp-image-175 size-medium" src="https://sites.uw.edu/biochemcore/files/2023/02/20230210_113442-300x146.jpg" alt="SEC-MALS" width="300" height="146" srcset="https://sites.uw.edu/biochemcore/files/2023/02/20230210_113442-300x146.jpg 300w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113442-1024x498.jpg 1024w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113442-768x373.jpg 768w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113442-1536x746.jpg 1536w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113442-375x182.jpg 375w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113442-750x364.jpg 750w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113442-1140x554.jpg 1140w, https://sites.uw.edu/biochemcore/files/2023/02/20230210_113442.jpg 1920w" sizes="auto, (max-width: 300px) 100vw, 300px" /></a></u></td> <td style="width: 79.6419%;">Currently Offline</p> <p><span style="color: #999999;"><u>SEC-MALS.</u> A Wyatt miniDAWN TREOS multi-angle light scattering detector coupled with an Optilab T-rEX differential refractive index detector is available for accurate and sensitive measurements of protein molecular weight (200 Da to 10 MDa) and radius of gyration (10 to 50 nm). An additional WyattQELS dynamic light scattering module allows for simultaneous measurement of the hydrodynamic radius. The light scattering instruments are downstream of an Agilent 1200-series HPLC chromatography system equipped with small-volume, high-resolution, analytical-grade size exclusion columns and an autosampler for running multiple samples.</span></p> <p><a href="https://www.wyatt.com/library/theory/understanding-multi-angle-static-light-scattering.html">https://www.wyatt.com/library/theory/understanding-multi-angle-static-light-scattering.html</a></td> </tr> <tr> <td style="width: 20.3581%;"> </p> <p> </td> <td style="width: 79.6419%;">Avestin Homogenizer C-3. Currently Unavailable. Future access TBD</p> <p><a href="https://www.avestin.com/emulsiflex-c3.htm">https://www.avestin.com/emulsiflex-c3.htm</a></td> </tr> </tbody> </table> <p> </p> <p> </p> <p> </p> </div> </div> <div id="sidebar"> <div class="col-md-4 uw-sidebar"> <nav id="desktop-relative" aria-label="mobile menu that is not visible in the desktop version"></nav> <div role="navigation" aria-label="sidebar_navigation" id="text-4" class="widget widget_text"> <div class="textwidget"><p> </p> <p> </p> </div> </div> <div role="navigation" aria-label="sidebar_navigation" id="contact-list-3" class="widget contact-widget"> <div class="contact-widget-inner"> <span> <h2 class="widgettitle">Contact</h2> </span> <h3 class='person-name'>Lisa Tuttle, PhD</h3><p class='person-title'>Facility Manager<br> Health Sciences Building K-407</p><p class='person-phone'></p><a href='mailto:biochemcore@uw.edu'class='person-email'>biochemcore@uw.edu</a> </div> </div><div role="navigation" aria-label="sidebar_navigation" id="uw-campus-map-3" class="widget uw-widget-campus-map"><div class="uw-campus-map-widget"> <iframe width="100%" height="305" src="//www.washington.edu/maps/embed/?code=hsk" style="border:0"></iframe> <a href="http://www.washington.edu/maps/#!/hsk" target="_blank">View larger</a> </div></div><div role="navigation" aria-label="sidebar_navigation" id="text-6" class="widget widget_text"> <div class="textwidget"><p> </p> <p> </p> </div> </div><div role="navigation" aria-label="sidebar_navigation" id="text-3" class="widget widget_text"><h2 class="widgettitle">Land Acknowledgment</h2> <div class="textwidget"><p>The University of Washington acknowledges the Coast Salish peoples of this land, the land which touches the shared waters of all tribes and bands within the Suquamish, Tulalip and Muckleshoot nations.</p> </div> </div> </div> </div> </div> </div> <div role="contentinfo" class="uw-footer"> <a href="http://www.washington.edu" class="footer-wordmark">University of Washington</a> <a href="http://www.washington.edu/boundless/"><h3 class="be-boundless">Be boundless</h3></a> <h4>Connect with us:</h4> <nav aria-label="social networking"> <ul class="footer-social"> <li><a class="facebook" href="http://www.facebook.com/UofWA">Facebook</a></li> <li><a class="twitter" href="http://twitter.com/UW">Twitter</a></li> <li><a class="instagram" href="http://instagram.com/uofwa">Instagram</a></li> <li><a class="youtube" href="http://www.youtube.com/user/uwhuskies">YouTube</a></li> <li><a class="linkedin" href="http://www.linkedin.com/company/university-of-washington">LinkedIn</a></li> <li><a class="pinterest" href="http://www.pinterest.com/uofwa/">Pinterest</a></li> <li><a class="google" href="https://plus.google.com/+universityofwashington/posts">Google+</a></li> </ul> </nav> <nav aria-label="footer navigation"> <ul class="footer-links"> <li><a href="http://www.uw.edu/accessibility">Accessibility</a></li> <li><a href="http://uw.edu/contact">Contact Us</a></li> <li><a href="http://www.washington.edu/jobs">Jobs</a></li> <li><a href="http://www.washington.edu/safety">Campus Safety</a></li> <li><a href="http://my.uw.edu/">My UW</a></li> <li><a href="http://www.washington.edu/rules/wac">Rules Docket</a></li> <li><a href="http://www.washington.edu/online/privacy/">Privacy</a></li> <li><a href="http://www.washington.edu/online/terms/">Terms</a></li> </ul> </nav> <p>© 2025 University of Washington | Seattle, WA</p> </div> </div> </div> <script type="text/javascript" src="https://sites.uw.edu/biochemcore/wp-includes/js/hoverintent-js.min.js?ver=2.2.1" id="hoverintent-js-js"></script> <script type="text/javascript" src="https://sites.uw.edu/biochemcore/wp-includes/js/admin-bar.min.js?ver=6.7.2" id="admin-bar-js"></script> <script type="text/javascript" id="blog_templates_front-js-extra"> /* <![CDATA[ */ var blog_templates_params = {"type":""}; 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