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GroEl</title> <meta name="description" content="Search results for: monomeric GroEl"> <meta name="keywords" content="monomeric GroEl"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="monomeric GroEl"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 34</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: monomeric GroEl</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">34</span> Folding Pathway and Thermodynamic Stability of Monomeric GroEL</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sarita%20Puri">Sarita Puri</a>, <a href="https://publications.waset.org/abstracts/search?q=Tapan%20K.%20Chaudhuri"> Tapan K. Chaudhuri</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Chaperonin GroEL is a tetradecameric Escherichia coli protein having identical subunits of 57 kDa. The elucidation of thermodynamic parameters related to stability for the native GroEL is not feasible as it undergoes irreversible unfolding because of its large size (800kDa) and multimeric nature. Nevertheless, it is important to determine the thermodynamic stability parameters for the highly stable GroEL protein as it helps in folding and holding of many substrate proteins during many cellular stresses. Properly folded monomers work as building-block for the formation of native tetradecameric GroEL. Spontaneous refolding behavior of monomeric GroEL makes it suitable for protein-denaturant interactions and thermodynamic stability based studies. The urea mediated unfolding is a three state process which means there is the formation of one intermediate state along with native and unfolded states. The heat mediated denaturation is a two-state process. The unfolding process is reversible as observed by the spontaneous refolding of denatured protein in both urea and head mediated refolding processes. Analysis of folding/unfolding data provides a measure of various thermodynamic stability parameters for the monomeric GroEL. The proposed mechanism of unfolding of monomeric GroEL is a three state process which involves formation of one stable intermediate having folded apical domain and unfolded equatorial, intermediate domains. Research in progress is to demonstrate the importance of specific residues in stability and oligomerization of GroEL protein. Several mutant versions of GroEL are under investigation to resolve the above mentioned issue. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=equilibrium%20unfolding" title="equilibrium unfolding">equilibrium unfolding</a>, <a href="https://publications.waset.org/abstracts/search?q=monomeric%20GroEl" title=" monomeric GroEl"> monomeric GroEl</a>, <a href="https://publications.waset.org/abstracts/search?q=spontaneous%20refolding" title=" spontaneous refolding"> spontaneous refolding</a>, <a href="https://publications.waset.org/abstracts/search?q=thermodynamic%20stability" title=" thermodynamic stability"> thermodynamic stability</a> </p> <a href="https://publications.waset.org/abstracts/67151/folding-pathway-and-thermodynamic-stability-of-monomeric-groel" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/67151.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">282</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">33</span> Investigation of the Variables Affecting the Use of Charcoal to Delay Fermentation in Wet Beans Slurry Using Chemical and Physical Analysis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Anuoluwapo%20O.%20Adewole">Anuoluwapo O. Adewole</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Fermentation is the conversion of monomeric sugars into ethanol and carbondioxide in the presence of microorganisms under anaerobic conditions. In line with the aim and objective of this research project, which is to investigate into the variables affecting the use of charcoal to delay fermentation in wet beans slurry, some physical and chemical analysis were carried out on the wet beans slurry using a PH meter in which a thermometer is incorporated in it, and a measuring cylinder was used for the foam level test. About 250 grams of the ground beans slurry was divided into two portions for testing. The sample with charcoal was labeled sample 'A' while the second sample without charcoal was labeled sample 'B' subsequently. The experiment lasted for a period of 41.15 hours (i.e., forty-one hours and nine minutes). During the fourth process, both samples could not be tested as the laboratory had been saturated with foul odor and both samples were packed and sealed in polythene bag for disposal in the trash can. It was generally observed that the sample with the charcoal lasted for a longer time before that without charcoal before total spoilage occurred. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fermentation" title="fermentation">fermentation</a>, <a href="https://publications.waset.org/abstracts/search?q=monomeric%20sugars" title=" monomeric sugars"> monomeric sugars</a>, <a href="https://publications.waset.org/abstracts/search?q=beans%20slurry" title=" beans slurry"> beans slurry</a>, <a href="https://publications.waset.org/abstracts/search?q=charcoal" title=" charcoal"> charcoal</a>, <a href="https://publications.waset.org/abstracts/search?q=anaerobic%20conditions" title=" anaerobic conditions"> anaerobic conditions</a> </p> <a href="https://publications.waset.org/abstracts/72057/investigation-of-the-variables-affecting-the-use-of-charcoal-to-delay-fermentation-in-wet-beans-slurry-using-chemical-and-physical-analysis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/72057.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">332</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">32</span> Aggregation of Fractal Aggregates Inside Fractal Cages in Irreversible Diﬀusion Limited Cluster Aggregation Binary Systems</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zakiya%20Shireen">Zakiya Shireen</a>, <a href="https://publications.waset.org/abstracts/search?q=Sujin%20B.%20Babu"> Sujin B. Babu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Irreversible diffusion-limited cluster aggregation (DLCA) of binary sticky spheres was simulated by modifying the Brownian Cluster Dynamics (BCD). We randomly distribute N spheres in a 3D box of size L, the volume fraction is given by Φtot = (π/6)N/L³. We identify NA and NB number of spheres as species A and B in our system both having identical size. In these systems, both A and B particles undergo Brownian motion. Irreversible bond formation happens only between intra-species particles and inter-species interact only through hard-core repulsions. As we perform simulation using BCD we start to observe binary gels. In our study, we have observed that species B always percolate (cluster size equal to L) as expected for the monomeric case and species A does not percolate below a critical ratio which is different for different volume fractions. We will also show that the accessible volume of the system increases when compared to the monomeric case, which means that species A is aggregating inside the cage created by B. We have also observed that for moderate Φtot the system undergoes a transition from ﬂocculation region to percolation region indicated by the change in fractal dimension from 1.8 to 2.5. For smaller ratio of A, it stays in the ﬂocculation regime even though B have already crossed over to the percolation regime. Thus, we observe two fractal dimension in the same system. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=BCD" title="BCD">BCD</a>, <a href="https://publications.waset.org/abstracts/search?q=fractals" title=" fractals"> fractals</a>, <a href="https://publications.waset.org/abstracts/search?q=percolation" title=" percolation"> percolation</a>, <a href="https://publications.waset.org/abstracts/search?q=sticky%20spheres" title=" sticky spheres"> sticky spheres</a> </p> <a href="https://publications.waset.org/abstracts/52269/aggregation-of-fractal-aggregates-inside-fractal-cages-in-irreversible-diffusion-limited-cluster-aggregation-binary-systems" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/52269.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">280</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">31</span> Extraction and Analysis of Anthocyanins Contents from Different Stage Flowers of the Orchids Dendrobium Hybrid cv. Ear-Sakul</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Orose%20Rugchati">Orose Rugchati</a>, <a href="https://publications.waset.org/abstracts/search?q=Khumthong%20Mahawongwiriya"> Khumthong Mahawongwiriya</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Dendrobium hybrid cv. Ear-Sakul has become one of the important commercial commodities in Thailand agricultural industry worldwide, either as potted plants or as cut flowers due to the attractive color produced in flower petals. Anthocyanins are the main flower pigments and responsible for the natural attractive display of petal colors. These pigments play an important role in functionality, such as to attract animal pollinators, classification, and grading of these orchids. Dendrobium hybrid cv. Ear-Sakul has been collected from local area farm in different stage flowers (F1, F2-F5, and F6). Anthocyanins pigment were extracted from the fresh flower by solvent extraction (MeOH–TFA 99.5:0.5v/v at 4ºC) and purification with ethyl acetate. The main anthocyanins components are cyanidin, pelargonidin, and delphinidin. Pure anthocyanin contents were analysis by UV-Visible spectroscopy technique at λ max 535, 520 and 546 nm respectively. The anthocyanins contents were converted in term of monomeric anthocyanins pigment (mg/L). The anthocyanins contents of all sample were compared with standard pigments cyanidin, pelargonidin and delphinidin. From this experiment is a simple extraction and analysis anthocyanins content in different stage of flowers results shown that monomeric anthocyanins pigment contents of different stage flowers (F1, F2-F5 and F6 ): cyanidin – 3 – glucoside (mg/l) are 0.85+0.08, 24.22+0.12 and 62.12+0.6; Pelargonidin 3,5-di- glucoside(mg/l) 10.37+0.12, 31.06+0.8 and 81.58+ 0.5; Delphinidin (mg/l) 6.34+0.17, 18.98+0.56 and 49.87+0.7; and the appearance of extraction pure anthocyanins in L(a, b): 2.71(1.38, -0.48), 1.06(0.39,-0.66) and 2.64(2.71,-3.61) respectively. Dendrobium Hybrid cv. Ear-Sakul could be used as a source of anthocyanins by simple solvent extraction and stage of flowers as a guideline for the prediction amount of main anthocyanins components are cyanidin, pelargonidin, and delphinidin could be application and development in quantities, and qualities with the advantage for food pharmaceutical and cosmetic industries. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=analysis" title="analysis">analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=anthocyanins%20contents" title=" anthocyanins contents"> anthocyanins contents</a>, <a href="https://publications.waset.org/abstracts/search?q=different%20stage%20flowers" title=" different stage flowers"> different stage flowers</a>, <a href="https://publications.waset.org/abstracts/search?q=Dendrobium%20Hybrid%20cv.%20Ear-Sakul" title=" Dendrobium Hybrid cv. Ear-Sakul"> Dendrobium Hybrid cv. Ear-Sakul</a> </p> <a href="https://publications.waset.org/abstracts/52719/extraction-and-analysis-of-anthocyanins-contents-from-different-stage-flowers-of-the-orchids-dendrobium-hybrid-cv-ear-sakul" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/52719.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">150</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">30</span> Management Prospects of Winery By-Products Based on Phenolic Compounds and Antioxidant Activity of Grape Skins: The Case of Greek Ionian Islands</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Marinos%20Xagoraris">Marinos Xagoraris</a>, <a href="https://publications.waset.org/abstracts/search?q=Iliada%20K.%20Lappa"> Iliada K. Lappa</a>, <a href="https://publications.waset.org/abstracts/search?q=Charalambos%20Kanakis"> Charalambos Kanakis</a>, <a href="https://publications.waset.org/abstracts/search?q=Dimitra%20Daferera"> Dimitra Daferera</a>, <a href="https://publications.waset.org/abstracts/search?q=Christina%20Papadopoulou"> Christina Papadopoulou</a>, <a href="https://publications.waset.org/abstracts/search?q=Georgios%20Sourounis"> Georgios Sourounis</a>, <a href="https://publications.waset.org/abstracts/search?q=Charilaos%20Giotis"> Charilaos Giotis</a>, <a href="https://publications.waset.org/abstracts/search?q=Pavlos%20Bouchagier"> Pavlos Bouchagier</a>, <a href="https://publications.waset.org/abstracts/search?q=Christos%20S.%20Pappas"> Christos S. Pappas</a>, <a href="https://publications.waset.org/abstracts/search?q=Petros%20A.%20Tarantilis"> Petros A. Tarantilis</a>, <a href="https://publications.waset.org/abstracts/search?q=Efstathia%20Skotti"> Efstathia Skotti</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The aim of this work was to recover phenolic compounds from grape skins produced in Greek varieties of the Ionian Islands in order to form the basis of calculations for their further utilization in the context of the circular economy. Isolation and further utilization of phenolic compounds is an important issue in winery by-products. For this purpose, 37 samples were collected, extracted, and analyzed in an attempt to provide the appropriate basis for their sustainable exploitation. Extraction of the bioactive compounds was held using an eco-friendly, non-toxic, and highly effective water-glycerol solvent system. Then, extracts were analyzed using UV-Vis, liquid chromatography-mass spectrometry (LC-MS), FTIR, and Raman spectroscopy. Also, total phenolic content and antioxidant activity were measured. LC-MS chromatography showed qualitative differences between different varieties. Peaks were attributed to monomeric 3-flavanols as well as monomeric, dimeric, and trimeric proanthocyanidins. The FT-IR and Raman spectra agreed with the chromatographic data and contributed to identifying phenolic compounds. Grape skins exhibited high total phenolic content (TPC), and it was proved that during vinification, a large number of polyphenols remained in the pomace. This study confirmed that grape skins from Ionian Islands are a promising source of bioactive compounds, suggesting their utilization under a bio-economic and environmental strategic framework. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antioxidant%20activity" title="antioxidant activity">antioxidant activity</a>, <a href="https://publications.waset.org/abstracts/search?q=grape%20skin" title=" grape skin"> grape skin</a>, <a href="https://publications.waset.org/abstracts/search?q=phenolic%20compounds" title=" phenolic compounds"> phenolic compounds</a>, <a href="https://publications.waset.org/abstracts/search?q=waste%20recovery" title=" waste recovery"> waste recovery</a> </p> <a href="https://publications.waset.org/abstracts/134702/management-prospects-of-winery-by-products-based-on-phenolic-compounds-and-antioxidant-activity-of-grape-skins-the-case-of-greek-ionian-islands" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/134702.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">148</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">29</span> Using of Bimolecular Fluorescence Complementation (BiFC) Assays to Study Homo and/ or Heterodimerization of Laminin Receptor 37 LRP/ 67 LR with Galectin-3</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Fulwah%20Alqahtani">Fulwah Alqahtani</a>, <a href="https://publications.waset.org/abstracts/search?q=Jafar%20Mahdavi"> Jafar Mahdavi</a>, <a href="https://publications.waset.org/abstracts/search?q=Lee%20Weldon"> Lee Weldon</a>, <a href="https://publications.waset.org/abstracts/search?q=Nick%20Holliday"> Nick Holliday</a>, <a href="https://publications.waset.org/abstracts/search?q=Dlawer%20Ala%27Aldeen"> Dlawer Ala'Aldeen</a> </p> <p class="card-text"><strong>Abstract:</strong></p> There are two isoforms of laminin receptor; monomeric 37 kDa laminin receptor precursor (37 LRP) and mature 67 kDa laminin receptor (67 LR). The relationship between the 67 LR and its precursor 37 LRP is not completely understood, but previous observations have suggested that 37 LRP can undergo homo- and/or hetero- dimerization with Galectin-3 (Gal-3) to form mature 67 LR. Gal-3 is the only member of the chimera-type group of galectins, and has one C-terminal carbohydrate recognition domain (CRD) that is responsible for binding the ß-galactoside moieties of mono- or oligosaccharides on several host and microbial molecules. The aim of this work was to investigate homo- and hetero-dimerization among the 37 LRP and Gal-3 to form mature 67 LR in mammalian cells using bimolecular fluorescence complementation (BiFC). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=37%20LRP" title="37 LRP">37 LRP</a>, <a href="https://publications.waset.org/abstracts/search?q=67%20LR" title=" 67 LR"> 67 LR</a>, <a href="https://publications.waset.org/abstracts/search?q=Gal-3" title=" Gal-3"> Gal-3</a>, <a href="https://publications.waset.org/abstracts/search?q=BiFC" title=" BiFC"> BiFC</a> </p> <a href="https://publications.waset.org/abstracts/15423/using-of-bimolecular-fluorescence-complementation-bifc-assays-to-study-homo-and-or-heterodimerization-of-laminin-receptor-37-lrp-67-lr-with-galectin-3" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/15423.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">504</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28</span> Polyacrylate Modified Copper Nanoparticles with Controlled Size</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Robert%20Prucek">Robert Prucek</a>, <a href="https://publications.waset.org/abstracts/search?q=Ale%C5%A1%20Pan%C3%A1%C4%8Dek"> Aleš Panáček</a>, <a href="https://publications.waset.org/abstracts/search?q=Jan%20Filip"> Jan Filip</a>, <a href="https://publications.waset.org/abstracts/search?q=Libor%20Kv%C3%ADtek"> Libor Kvítek</a>, <a href="https://publications.waset.org/abstracts/search?q=Radek%20Zbo%C5%99il"> Radek Zbořil</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The preparation of Cu nanoparticles (NPs) through the reduction of copper ions by sodium borohydride in the presence of sodium polyacrylate with a molecular weight of 1200 is reported. Cu NPs were synthesized at a concentration of copper salt equal to 2.5, 5, and 10 mM, and at a molar ratio of copper ions and monomeric unit of polyacrylate equal to 1:2. The as-prepared Cu NPs have diameters of about 2.5–3 nm for copper concentrations of 2.5 and 5 mM, and 6 nm for copper concentration of 10 mM. Depending on the copper salt concentration and concentration of additionally added polyacrylate to Cu particle dispersion, primarily formed NPs grow through the process of aggregation and/or coalescence into clusters and/or particles with a diameter between 20–100 nm. The amount of additionally added sodium polyacrylate influences the stability of Cu particles against air oxidation. The catalytic efficiency of the prepared Cu particles for the reduction of 4-nitrophenol is discussed. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=copper" title="copper">copper</a>, <a href="https://publications.waset.org/abstracts/search?q=nanoparticles" title=" nanoparticles"> nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=sodium%20polyacrylate" title=" sodium polyacrylate"> sodium polyacrylate</a>, <a href="https://publications.waset.org/abstracts/search?q=catalyst" title=" catalyst"> catalyst</a>, <a href="https://publications.waset.org/abstracts/search?q=4-nitrophenol" title=" 4-nitrophenol"> 4-nitrophenol</a> </p> <a href="https://publications.waset.org/abstracts/6486/polyacrylate-modified-copper-nanoparticles-with-controlled-size" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/6486.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">277</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27</span> Evaluation of Differential Interaction between Flavanols and Saliva Proteins by Diffusion and Precipitation Assays on Cellulose Membranes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=E.%20Obreque-Slier">E. Obreque-Slier</a>, <a href="https://publications.waset.org/abstracts/search?q=V.%20Contreras-Cortez"> V. Contreras-Cortez</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20L%C3%B3pez-Sol%C3%ADs"> R. López-Solís</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Astringency is a drying, roughing, and sometimes puckering sensation that is experienced on the various oral surfaces during or immediately after tasting foods. This sensation has been closely related to the interaction and precipitation between salivary proteins and polyphenols, specifically flavanols or proanthocyanidins. In addition, the type and concentration of proanthocyanidin influences significantly the intensity of the astringency and consequently the protein/proanthocyanidin interaction. However, most of the studies are based on the interaction between saliva and highly complex polyphenols, without considering the effect of monomeric proanthoancyanidins present in different foods. The aim of this study was to evaluate the effect of different monomeric proanthocyanidins on the diffusion and precipitation of salivary proteins. Thus, solutions of catechin, epicatechin, epigallocatechin and gallocatechin (0, 2.0, 4.0, 6.0, 8.0 and 10 mg/mL) were mixed with human saliva (1: 1 v/v). After incubation for 5 min at room temperature, 15 µL aliquots of each mix were dotted on a cellulose membrane and allowed to dry spontaneously at room temperature. The membrane was fixed, rinsed and stained for proteins with Coomassie blue. After exhaustive washing in 7% acetic acid, the membrane was rinsed once in distilled water and dried under a heat lamp. Both diffusion area and stain intensity of the protein spots were semiqualitative estimates for protein-tannin interaction (diffusion test). The rest of the whole saliva-phenol solution mixtures of the diffusion assay were centrifuged, and 15-μL aliquots from each of the supernatants were dotted on a cellulose membrane. The membrane was processed for protein staining as indicated above. The blue-stained area of protein distribution corresponding to each of the extract dilution-saliva mixtures was quantified by Image J 1.45 software. Each of the assays was performed at least three times. Initially, salivary proteins display a biphasic distribution on cellulose membranes, that is, when aliquots of saliva are placed on absorbing cellulose membranes, and free diffusion of saliva is allowed to occur, a non-diffusible protein fraction becomes surrounded by highly diffusible salivary proteins. In effect, once diffusion has ended, a protein-binding dye shows an intense blue-stained roughly circular area close to the spotting site (non-diffusible fraction) (NDF) which becomes surrounded by a weaker blue-stained outer band (diffusible fraction) (DF). Likewise, the diffusion test showed that epicatechin caused the complete disappearance of DF from saliva with 2 mg/mL. Also, epigallocatechin and gallocatechin caused a similar effect with 4 mg/mL, while catechin generated the same effect at 8 mg/mL. In the precipitation test, the use of epicatechin and gallocatechin generated evident precipitates at the bottom of the Eppendorf tubes. In summary, the flavanol type differentially affects the diffusion and precipitation of saliva, which would affect the sensation of astringency perceived by consumers. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=astringency" title="astringency">astringency</a>, <a href="https://publications.waset.org/abstracts/search?q=polyphenols" title=" polyphenols"> polyphenols</a>, <a href="https://publications.waset.org/abstracts/search?q=tannins" title=" tannins"> tannins</a>, <a href="https://publications.waset.org/abstracts/search?q=tannin-protein%20interaction" title=" tannin-protein interaction"> tannin-protein interaction</a> </p> <a href="https://publications.waset.org/abstracts/75815/evaluation-of-differential-interaction-between-flavanols-and-saliva-proteins-by-diffusion-and-precipitation-assays-on-cellulose-membranes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/75815.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">199</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">26</span> The Four-Way Interactions among Host Plant-Whitefly-Virus-Endosymbionts in Insect and Disease Development</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=N.%20R.%20Prasannakumar">N. R. Prasannakumar</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20N.%20Maruthi"> M. N. Maruthi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The whitefly, Bemisia tabaci (Gennadius) (Hemiptera; Aleyrodidae) is a highly polyphagous pest reported to infest over 600 plant hosts globally. About 42 genetic groups/cryptic species of B. tabaci exist in the world on different hosts. The species have variable behaviour with respect to feeding, development and transmission of viral diseases. Feeding on diverse host plants affect both whitefly development and the population of the endosymbionts harboured by the insects. Due to changes in the level of endosymbionts, the virus transmission efficiency by the vector also gets affected. We investigated these interactions on five host plants – egg plant, tomato, beans, okra and cotton - using a single whitefly species Asia 1 infected with three different bacteria Portiera, Wolbachia and Arsenophonus. The Asia 1 transmits the Tomato leaf curl Bangalore virus (ToLCBV) effectively and thus was used in the interaction studies. We found a significant impact of hosts on whitefly growth and development; eggplant was most favourable host, while okra and tomato were least favourable. Among the endosymbiotic bacteria, the titre of Wolbachia was significantly affected by feeding of B. tabaci on different host plants whereas Arsenophonus and Portiera were unaffected. When whitefly fed on ToLCBV-infected tomato plants, the Arsenophonus population was significantly increased, indicating its previously confirmed role in ToLCBV transmission. Further, screening of total proteins of B. tabaci Asia 1 genetic group interacting with ToLCBV coat protein was carried out using Y2H system. Some of the proteins found to be interacting with ToLCBV CP were HSPs 70kDa, GroEL, nucleoproteins, vitellogenins, apolipophorins, lachesins, enolase. The reported protein thus would be the potential targets for novel whitefly control strategies such as RNAi or novel insecticide target sites for sustainable whitefly management after confirmation of genuine proteins. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cDNA" title="cDNA">cDNA</a>, <a href="https://publications.waset.org/abstracts/search?q=whitefly" title=" whitefly"> whitefly</a>, <a href="https://publications.waset.org/abstracts/search?q=ToLCBV" title=" ToLCBV"> ToLCBV</a>, <a href="https://publications.waset.org/abstracts/search?q=endosymbionts" title=" endosymbionts"> endosymbionts</a>, <a href="https://publications.waset.org/abstracts/search?q=Y2H" title=" Y2H"> Y2H</a> </p> <a href="https://publications.waset.org/abstracts/119847/the-four-way-interactions-among-host-plant-whitefly-virus-endosymbionts-in-insect-and-disease-development" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/119847.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">115</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">25</span> The Extension of Monomeric Computational Results to Polymeric Measurable Properties: An Introductory Computational Chemistry Experiment</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jing%20Zhao">Jing Zhao</a>, <a href="https://publications.waset.org/abstracts/search?q=Yongqing%20Bai"> Yongqing Bai</a>, <a href="https://publications.waset.org/abstracts/search?q=Qiaofang%20Shi"> Qiaofang Shi</a>, <a href="https://publications.waset.org/abstracts/search?q=Huaihao%20Zhang"> Huaihao Zhang</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Advances in software technology enable computational chemistry to be commonly applied in various research fields, especially in pedagogy. Thus, in order to expand and improve experimental instructions of computational chemistry for undergraduates, we designed an introductory experiment—research on acrylamide molecular structure and physicochemical properties. Initially, students construct molecular models of acrylamide and polyacrylamide in Gaussian and Materials Studio software respectively. Then, the infrared spectral data, atomic charge and molecular orbitals of acrylamide as well as solvation effect of polyacrylamide are calculated to predict their physicochemical performance. At last, rheological experiments are used to validate these predictions. Through the combination of molecular simulation (performed on Gaussian, Materials Studio) with experimental verification (rheology experiment), learners have deeply comprehended the chemical nature of acrylamide and polyacrylamide, achieving good learning outcomes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=upper-division%20undergraduate" title="upper-division undergraduate">upper-division undergraduate</a>, <a href="https://publications.waset.org/abstracts/search?q=computer-based%20learning" title=" computer-based learning"> computer-based learning</a>, <a href="https://publications.waset.org/abstracts/search?q=laboratory%20instruction" title=" laboratory instruction"> laboratory instruction</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20modeling" title=" molecular modeling"> molecular modeling</a> </p> <a href="https://publications.waset.org/abstracts/144030/the-extension-of-monomeric-computational-results-to-polymeric-measurable-properties-an-introductory-computational-chemistry-experiment" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/144030.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">133</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">24</span> Spectroscopic, Molecular Structure and Electrostatic Potential, Polarizability, Hyperpolarizability, and HOMO–LUMO Analysis of Monomeric and Dimeric Structures of N-(2-Methylphenyl)-2-Nitrobenzenesulfonamide </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20Didaoui">A. Didaoui</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Benhalima"> N. Benhalima</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Elkeurti"> M. Elkeurti</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Chouaih"> A. Chouaih</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Hamzaoui"> F. Hamzaoui </a> </p> <p class="card-text"><strong>Abstract:</strong></p> The monomer and dimer structures of the title molecule have been obtained from density functional theory (DFT) B3LYP method with 6-31G (d,p) as basis set calculations. The optimized geometrical parameters obtained by B3LYP/6-31G (d,p) method show good agreement with xperimental X-ray data. The polarizability and first order hyperpolarizabilty of the title molecule were calculated and interpreted. the intermolecular N–H•••O hydrogen bonds are discussed in dimer structure of the molecule. The vibrational wave numbers and their assignments were examined theoretically using the Gaussian 03 set of quantum chemistry codes. The predicted frontier molecular orbital energies at B3LYP/6-31G(d,p) method set show that charge transfer occurs within the molecule. The frontier molecular orbital calculations clearly show the inverse relationship of HOMO–LUMO gap with the total static hyperpolarizability. The results also show that N-(2-Methylphenyl)-2-nitrobenzenesulfonamide molecule may have nonlinear optical (NLO) comportment with non-zero values. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=DFT" title="DFT">DFT</a>, <a href="https://publications.waset.org/abstracts/search?q=Gaussian%2003" title=" Gaussian 03"> Gaussian 03</a>, <a href="https://publications.waset.org/abstracts/search?q=NLO" title=" NLO"> NLO</a>, <a href="https://publications.waset.org/abstracts/search?q=N-%282-Methylphenyl%29-2-nitrobenzenesulfonamide" title=" N-(2-Methylphenyl)-2-nitrobenzenesulfonamide"> N-(2-Methylphenyl)-2-nitrobenzenesulfonamide</a> </p> <a href="https://publications.waset.org/abstracts/19477/spectroscopic-molecular-structure-and-electrostatic-potential-polarizability-hyperpolarizability-and-homo-lumo-analysis-of-monomeric-and-dimeric-structures-of-n-2-methylphenyl-2-nitrobenzenesulfonamide" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/19477.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">549</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">23</span> The Spectroscopic, Molecular Structure and Electrostatic Potential, Polarizability Hyperpolarizability, and Homo–Lumo Analysis of Monomeric and Dimeric Structures of 2-Chloro-N-(2 Methylphenyl) Benzamide</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=N.%20Khelloul">N. Khelloul</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Benhalima"> N. Benhalima</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Chouaih"> A. Chouaih</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Hamzaoui"> F. Hamzaoui</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The monomer and dimer structures of the title molecule have been obtained from density functional theory (DFT) B3LYP method with 6-31G (d,p) as basis set calculations. The optimized geometrical parameters obtained by B3LYP/6-31G (d,p) method shows good agreement with experimental X-ray data. The polarizability and first order hyperpolarizabilty of the title molecule were calculated and interpreted. The intermolecular N–H•••O hydrogen bonds are discussed in dimer structure of the molecule. The vibrational wave numbers and their assignments were examined theoretically using the Gaussian 09 set of quantum chemistry codes. The predicted frontier molecular orbital energies at B3LYP/6-31G(d,p) method set show that charge transfer occurs within the molecule. The frontier molecular orbital calculations clearly show the inverse relationship of HOMO–LUMO gap with the total static hyperpolarizability. The results also show that 2-Chloro-N-(2-methylphenyl) benzamide 2 molecule may have nonlinear optical (NLO) comportment with non-zero values. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=DFT" title="DFT">DFT</a>, <a href="https://publications.waset.org/abstracts/search?q=HOMO" title=" HOMO"> HOMO</a>, <a href="https://publications.waset.org/abstracts/search?q=LUMO" title=" LUMO"> LUMO</a>, <a href="https://publications.waset.org/abstracts/search?q=NLO" title=" NLO "> NLO </a> </p> <a href="https://publications.waset.org/abstracts/40183/the-spectroscopic-molecular-structure-and-electrostatic-potential-polarizability-hyperpolarizability-and-homo-lumo-analysis-of-monomeric-and-dimeric-structures-of-2-chloro-n-2-methylphenyl-benzamide" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/40183.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">339</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">22</span> The Spectroscopic, Molecular Structure and Electrostatic Potential, Polarizability, Hyperpolarizability, and HOMO–LUMO Analysis of Monomeric and Dimeric Structures of N-(2-Methylphenyl)-2-Nitrobenzenesulfonamide</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20Didaoui">A. Didaoui</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Benhalima"> N. Benhalima</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Elkeurti"> M. Elkeurti</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Chouaih"> A. Chouaih</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Hamzaoui"> F. Hamzaoui</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The monomer and dimer structures of the title molecule have been obtained from density functional theory (DFT) B3LYP method with 6-31G(d,p) as basis set calculations. The optimized geometrical parameters obtained by B3LYP/6-31G(d,p) method show good agreement with experimental X-ray data. The polarizability and first order hyperpolarizability of the title molecule were calculated and interpreted. The intermolecular N–H•••O hydrogen bonds are discussed in dimer structure of the molecule. The vibrational wave numbers and their assignments were examined theoretically using the Gaussian 03 set of quantum chemistry codes. The predicted frontier molecular orbital energies at B3LYP/6-31G(d,p) method set show that charge transfer occurs within the molecule. The frontier molecular orbital calculations clearly show the inverse relationship of HOMO–LUMO gap with the total static hyperpolarizability. The results also show that N-(2-Methylphenyl)-2-nitrobenzenesulfonamide molecule may have nonlinear optical (NLO) comportment with non-zero values. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=DFT" title="DFT">DFT</a>, <a href="https://publications.waset.org/abstracts/search?q=Gaussian%2003" title=" Gaussian 03"> Gaussian 03</a>, <a href="https://publications.waset.org/abstracts/search?q=NLO" title=" NLO"> NLO</a>, <a href="https://publications.waset.org/abstracts/search?q=N-%282-Methylphenyl%29-2-nitrobenzenesulfonamide" title=" N-(2-Methylphenyl)-2-nitrobenzenesulfonamide"> N-(2-Methylphenyl)-2-nitrobenzenesulfonamide</a>, <a href="https://publications.waset.org/abstracts/search?q=polarizability" title=" polarizability"> polarizability</a> </p> <a href="https://publications.waset.org/abstracts/18951/the-spectroscopic-molecular-structure-and-electrostatic-potential-polarizability-hyperpolarizability-and-homo-lumo-analysis-of-monomeric-and-dimeric-structures-of-n-2-methylphenyl-2-nitrobenzenesulfonamide" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/18951.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">325</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">21</span> Gold–M Heterobimetallic Complexes: Synthesis and Initial Reactivity Studies</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Caroline%20Alice%20Rouget-Virbel">Caroline Alice Rouget-Virbel</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Dean%20Toste"> F. Dean Toste</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Heterobimetallic systems have been precedented in a wide array of bioinorganic and heterogeneous catalytic settings, in which cooperative bond-breaking and bond-forming events mediated by neighboring metal sites have been proposed but are challenging to study and characterize. Heterodinuclear transition-metal catalysis has recently emerged as a promising strategy to tackle challenging chemical transformations, including C−C and C−X couplings as well as small molecule activation. It has been shown that these reactions can traverse nontraditional mechanisms, reactivities, and selectivities when homo- and heterobimetallic systems are employed. Moreover, stoichiometric studies of transmetallation from gold complexes have demonstrated that R transfer from PPh3–Au(I)R to Cp- and Cp*-ligated group 8/9 complexes is a viable elementary step. With these considerations in mind, we hypothesized that heterobimetallic Au–M complexes could serve as a viable and tunable catalyst platform to explore mechanisms and reactivity. In this work, heterobimetallic complexes containing Au(I) centers tethered to Ir(III) and Rh(III) piano stool moieties were synthesized and characterized. Preliminary application of these complexes to a catalytic allylic arylation reaction demonstrates bimetallic cooperativity relative to their monomeric metal components. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=heterobimetallic" title="heterobimetallic">heterobimetallic</a>, <a href="https://publications.waset.org/abstracts/search?q=catalysis" title=" catalysis"> catalysis</a>, <a href="https://publications.waset.org/abstracts/search?q=gold" title=" gold"> gold</a>, <a href="https://publications.waset.org/abstracts/search?q=rhodium" title=" rhodium"> rhodium</a> </p> <a href="https://publications.waset.org/abstracts/139787/gold-m-heterobimetallic-complexes-synthesis-and-initial-reactivity-studies" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/139787.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">183</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">20</span> Optimization of Pyrogallol Based Manganese / Ferroin Catalyzed Nonlinear Chemical Systems and Interaction with Monomeric and Polymeric Entities</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ghulam%20Mustafa%20Peerzada">Ghulam Mustafa Peerzada</a>, <a href="https://publications.waset.org/abstracts/search?q=Shagufta%20Rashid"> Shagufta Rashid</a>, <a href="https://publications.waset.org/abstracts/search?q=Nadeem%20Bashir"> Nadeem Bashir</a> </p> <p class="card-text"><strong>Abstract:</strong></p> These the influence of initial reagent concentrations on the Belousov-Zhabotinsky (BZ) system with Mn2+/Mn3+ as redox catalyst, inorganic bromate as oxidant and pyrogallol as organic substrate was studied. The reactions were monitored by potentiometery in oxidation reduction potential (ORP) mode. The aforesaid reagents were mixed with varying concentrations to evolve the optimal concentrations at which the reaction system exhibited better oscillations. The various oscillatory parameters such as induction period (tin), time period (tp), frequency (v), amplitude (A) and number of oscillations (n) were derived and the dependence of concentration of the reacting species on these oscillatory parameters was interpreted on the basis of the Field-Koros-Noyes mechanism. Ferroin based BZ system with pyrogallol as organic substrate was optimized under CSTR condition at temperature of 30±0.1oC Effect of molecules like monomer and polymer as additives to the system was checked and their interaction with the system was also studied. It has been observed that the monomer affects the time period, while the polymer has its effect on the amplitude of oscillations because of monomer’s interaction with the bromine and polymer’s with that of the Ferroin. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Belousov%20Zhabotinsky%20reaction" title="Belousov Zhabotinsky reaction">Belousov Zhabotinsky reaction</a>, <a href="https://publications.waset.org/abstracts/search?q=oscillatory%20parameters" title=" oscillatory parameters"> oscillatory parameters</a>, <a href="https://publications.waset.org/abstracts/search?q=polymer" title=" polymer"> polymer</a>, <a href="https://publications.waset.org/abstracts/search?q=pyrogallol" title=" pyrogallol"> pyrogallol</a> </p> <a href="https://publications.waset.org/abstracts/28993/optimization-of-pyrogallol-based-manganese-ferroin-catalyzed-nonlinear-chemical-systems-and-interaction-with-monomeric-and-polymeric-entities" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/28993.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">312</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">19</span> Insights of Interaction Studies between HSP-60, HSP-70 Proteins and HSF-1 in Bubalus bubalis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ravinder%20Singh">Ravinder Singh</a>, <a href="https://publications.waset.org/abstracts/search?q=C%20Rajesh"> C Rajesh</a>, <a href="https://publications.waset.org/abstracts/search?q=Saroj%20Badhan"> Saroj Badhan</a>, <a href="https://publications.waset.org/abstracts/search?q=Shailendra%20Mishra"> Shailendra Mishra</a>, <a href="https://publications.waset.org/abstracts/search?q=Ranjit%20Singh%20Kataria"> Ranjit Singh Kataria</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Heat shock protein 60 and 70 are crucial chaperones that guide appropriate folding of denatured proteins under heat stress conditions. HSP60 and HSP70 provide assistance in correct folding of a multitude of denatured proteins. The heat shock factors are the family of some transcription factors which controls the regulation of gene expression of proteins involved in folding of damaged or improper folded proteins during stress conditions. Under normal condition heat shock proteins bind with HSF-1 and act as its repressor as well as aids in maintaining the HSF-1’s nonactive and monomeric confirmation. The experimental protein structure for all these proteins in Bubalus bubalis is not known till date. Therefore computational approach was explored to identify three-dimensional structure analysis of all these proteins. In this study, an extensive in silico analysis has been performed including sequence comparison among species to comparative modeling of Bubalus bubalis HSP60, HSP70 and HSF-1 protein. The stereochemical properties of proteins were assessed by utilizing several scrutiny bioinformatics tools to ensure model accuracy. Further docking approach was used to study interactions between Heat shock proteins and HSF-1. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bubalus%20bubalis" title="Bubalus bubalis">Bubalus bubalis</a>, <a href="https://publications.waset.org/abstracts/search?q=comparative%20modelling" title=" comparative modelling"> comparative modelling</a>, <a href="https://publications.waset.org/abstracts/search?q=docking" title=" docking"> docking</a>, <a href="https://publications.waset.org/abstracts/search?q=heat%20shock%20protein" title=" heat shock protein"> heat shock protein</a> </p> <a href="https://publications.waset.org/abstracts/64431/insights-of-interaction-studies-between-hsp-60-hsp-70-proteins-and-hsf-1-in-bubalus-bubalis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/64431.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">322</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">18</span> Purification, Biochemical Characterization and Application of an Extracellular Alkaline Keratinase Produced by Aspergillus sp. DHE7 </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dina%20Helmy%20%20El-Ghonemy">Dina Helmy El-Ghonemy</a>, <a href="https://publications.waset.org/abstracts/search?q=Thanaa%20Hamed%20Ali"> Thanaa Hamed Ali</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The aim of this study was to purify and characterize a keratinolytic enzyme produced by Aspergillus sp. DHE7 cultured in basal medium containing chicken feather as substrate. The enzyme was purified through ammonium sulfate saturation of 60%, followed by gel filtration chromatography in Sephadex G-100, with a 16.4-purification fold and recovery yield of 52.2%. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the purified enzyme is a monomeric enzyme with an apparent molecular mass of 30 kDa — the purified keratinase of Aspergillus sp. DHE7 exhibited activity in a broad range of pH (7- 9) and temperature (40℃-60℃) profiles with an optimal activity at pH eight and 50℃. The keratinolytic activity was inhibited by protease inhibitors such as phenylmethylsulfonyl fluoride and ethylenediaminetetraacetate, while no reduction of activity was detected by the addition of dimethyl sulfoxide (DMSO). Bivalent cations, Ca²⁺ and Mn²⁺, were able to greatly enhance the activity of keratinase by 125.7% and 194.8%, respectively, when used at one mM final concentration. On the other hand, Cu²⁺ and Hg²⁺ inhibited the enzyme activity, which might be indicative of essential vicinal sulfhydryl groups of the enzyme for productive catalysis. Furthermore, the purified keratinase showed significant stability and compatibility against the tested commercial detergents at 37ºC. Therefore, these results suggested that the purified keratinase from Aspergillus sp. DHE7 may have potential use in the detergent industry and should be of interest in the processing of poultry feather waste. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Aspergillus%20sp.%20DHE7" title="Aspergillus sp. DHE7">Aspergillus sp. DHE7</a>, <a href="https://publications.waset.org/abstracts/search?q=biochemical%20characterization" title=" biochemical characterization"> biochemical characterization</a>, <a href="https://publications.waset.org/abstracts/search?q=keratinase" title=" keratinase"> keratinase</a>, <a href="https://publications.waset.org/abstracts/search?q=purification" title=" purification"> purification</a>, <a href="https://publications.waset.org/abstracts/search?q=waste%20management" title=" waste management"> waste management</a> </p> <a href="https://publications.waset.org/abstracts/116947/purification-biochemical-characterization-and-application-of-an-extracellular-alkaline-keratinase-produced-by-aspergillus-sp-dhe7" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/116947.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">124</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">17</span> Sorption of Charged Organic Dyes from Anionic Hydrogels</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Georgios%20Linardatos">Georgios Linardatos</a>, <a href="https://publications.waset.org/abstracts/search?q=Miltiadis%20Zamparas"> Miltiadis Zamparas</a>, <a href="https://publications.waset.org/abstracts/search?q=Vlasoula%20Bekiari"> Vlasoula Bekiari</a>, <a href="https://publications.waset.org/abstracts/search?q=Georgios%20Bokias"> Georgios Bokias</a>, <a href="https://publications.waset.org/abstracts/search?q=Georgios%20Hotos"> Georgios Hotos</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Hydrogels are three-dimensional, hydrophilic, polymeric networks composed of homopolymers or copolymers and are insoluble in water due to the presence of chemical or physical cross-links. When hydrogels come in contact with aqueous solutions, they can effectively sorb and retain the dissolved substances, depending on the nature of the monomeric units comprising the hydrogel. For this reason, hydrogels have been proposed in several studies as water purification agents. At the present work anionic hydrogels bearing negatively charged –COO- groups were prepared and investigated. These gels are based on sodium acrylate (ANa), either homopolymerized (poly(sodiumacrylate), PANa) or copolymerized (P(DMAM-co-ANa)) with N,N Dimethylacrylamide (DMAM). The hydrogels were used to extract some model organic dyes from water. It is found that cationic dyes are strongly sorbed and retained by the hydrogels, while sorption of anionic dyes was negligible. In all cases it was found that both maximum sorption capacity and equilibrium binding constant varied from one dye to the other depending on the chemical structure of the dye, the presence of functional chemical groups and the hydrophobic-hydrophilic balance. Finally, the nonionic hydrogel of the homopolymer poly(N,N-dimethylacrylamide), PDMAM, was also used for reasons of comparison. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=anionic%20organic%20hydrogels" title="anionic organic hydrogels">anionic organic hydrogels</a>, <a href="https://publications.waset.org/abstracts/search?q=sorption" title=" sorption"> sorption</a>, <a href="https://publications.waset.org/abstracts/search?q=organic%20dyes" title=" organic dyes"> organic dyes</a>, <a href="https://publications.waset.org/abstracts/search?q=water%20purification%20agents" title=" water purification agents"> water purification agents</a> </p> <a href="https://publications.waset.org/abstracts/39319/sorption-of-charged-organic-dyes-from-anionic-hydrogels" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/39319.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">259</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">16</span> Synthesis of Rare-Earth Pyrazolate Compounds</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nazli%20Eslamirad">Nazli Eslamirad</a>, <a href="https://publications.waset.org/abstracts/search?q=Peter%20C.%20Junk"> Peter C. Junk</a>, <a href="https://publications.waset.org/abstracts/search?q=Jun%20Wang"> Jun Wang</a>, <a href="https://publications.waset.org/abstracts/search?q=Glen%20B.%20Deacon"> Glen B. Deacon</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Since coordination behavior of pyrazoles and pyrazolate ions are widely versatile towards a great range of metals such as d-block, f-block as well as main group elements; they attract interest as ligands for preparing compounds. A variety of rare-earth pyrazolate complexes have been synthesized by redox transmetalation/protolysis (RTP) previously, therefore, a variety of rare-earth pyrazolate complexes using two pyrazoles, 3,5-dimethylpyrazole (Me₂pzH) and 3,5-di-tert -butylpyrazolate (t-Bu₂pzH), in which the structures span the whole La-Lu array beside Sc and Y has been synthesized by RTP reaction. There have been further developments in this study: Synthesizing structure of [Tb(Me₂pz)₃(thf)]₂ which is isomorphous with those of the previously reported [Dy(Me₂pz)₃(thf)]₂ and [Lu(Me₂pz)₃(thf)]₂ analogous that has two µ-1(N):2(Nʹ)-Me2pz ligands (the most common pyrazolate ligation for non-rare-earth complexes). Previously most of the reported compounds using t-Bu2pzH were monomeric compounds however the lanthanum derivative [La(Me₂pz)₃thf₂] ,which has been reported previously without crystal structure, has now been structurally characterized, along with cerium and lutetium analogue. Also a polymeric structure with samarium has now been synthesized which the neodymium analogue has been reported previously and comparing these polymeric structures can support the idea that the geometry of Sm(tBu₂pz)₃ affect the coordination of the solvent. Also, by using 1,2-dimethoxyethane (DME) instead of tetrahydrofuran (THF) new [Er(tBu₂pz)₃ (dme)₂] has now been reported. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=lanthanoid%20complexes" title="lanthanoid complexes">lanthanoid complexes</a>, <a href="https://publications.waset.org/abstracts/search?q=pyrazolate" title=" pyrazolate"> pyrazolate</a>, <a href="https://publications.waset.org/abstracts/search?q=redox%20transmetalation%2Fprotolysis" title=" redox transmetalation/protolysis"> redox transmetalation/protolysis</a>, <a href="https://publications.waset.org/abstracts/search?q=x-ray%20crystal%20structures" title=" x-ray crystal structures"> x-ray crystal structures</a> </p> <a href="https://publications.waset.org/abstracts/74197/synthesis-of-rare-earth-pyrazolate-compounds" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/74197.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">220</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">15</span> Studies on Interaction between Anionic Polymer Sodium Carboxymethylcellulose with Cationic Gemini Surfactants</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20Kamil">M. Kamil</a>, <a href="https://publications.waset.org/abstracts/search?q=Rahber%20Husain%20Khan"> Rahber Husain Khan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the present study, the Interaction of anionic polymer, sodium carboxymethylcellulose (NaCMC), with cationic gemini surfactants 2,2[(oxybis(ethane-1,2-diyl))bis(oxy)]bis(N-hexadecyl1-N,N-[di(E2)/tri(E3)]methyl1-2-oxoethanaminium)chloride (16-E2-16 and 16-E3-16) and conventional surfactant (CTAC) in aqueous solutions have been studied by surface tension measurement of binary mixtures (0.0- 0.5 wt% NaCMC and 1 mM gemini surfactant/10 mM CTAC solution). Surface tension measurements were used to determine critical aggregation concentration (CAC) and critical micelle concentration (CMC). The maximum surface excess concentration (Ґmax) at the air-water interface was evaluated by the Gibbs adsorption equation. The minimum area per surfactant molecule was evaluated, which indicates the surfactant-polymer Interaction in a mixed system. The effect of changing surfactant chain length on CAC and CMC values of mixed polymer-surfactant systems was examined. From the results, it was found that the gemini surfactant interacts strongly with NaCMC as compared to its corresponding monomeric counterpart CTAC. In these systems, electrostatic interactions predominate. The lowering of surface tension with an increase in the concentration of surfactants is higher in the case of gemini surfactants almost 10-15 times. The measurements indicated that the Interaction between NaCMC-CTAC resulted in complex formation. The volume of coacervate increases with an increase in CTAC concentration; however, above 0.1 wt. % concentration coacervate vanishes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=anionic%20polymer" title="anionic polymer">anionic polymer</a>, <a href="https://publications.waset.org/abstracts/search?q=gemni%20surfactants" title=" gemni surfactants"> gemni surfactants</a>, <a href="https://publications.waset.org/abstracts/search?q=tensiometer" title=" tensiometer"> tensiometer</a>, <a href="https://publications.waset.org/abstracts/search?q=CMC" title=" CMC"> CMC</a>, <a href="https://publications.waset.org/abstracts/search?q=interaction" title=" interaction"> interaction</a> </p> <a href="https://publications.waset.org/abstracts/163366/studies-on-interaction-between-anionic-polymer-sodium-carboxymethylcellulose-with-cationic-gemini-surfactants" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/163366.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">89</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">14</span> Anti-Angiogenic Effects of the Macrovipera lebetina obtusa Snake Crude Venom and Obtustatin</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Narine%20Ghazaryan">Narine Ghazaryan</a>, <a href="https://publications.waset.org/abstracts/search?q=Joana%20Catarina%20Macedo"> Joana Catarina Macedo</a>, <a href="https://publications.waset.org/abstracts/search?q=Sara%20Vaz"> Sara Vaz</a>, <a href="https://publications.waset.org/abstracts/search?q=Naira%20Ayvazyan"> Naira Ayvazyan</a>, <a href="https://publications.waset.org/abstracts/search?q=Elsa%20Logarinho"> Elsa Logarinho</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Macrovipera lebetina obtusa (MLO) is a poisonous snake in Armenia. Obtustatin represents the shortest known monomeric disintegrin, isolated from the snake venom of MLO, and is known to specifically inhibit α1β1 integrin. Its oncostatic effect is due to the inhibition of angiogenesis, which likely arises from α1β1 integrin inhibition in the endothelial cells. To explore the therapeutic potential of the MLO snake venom and obtustatin, we studied activity of obtustatin and MLO venom in vitro, by testing their efficacy in human dermal microvascular endothelial cells (HMVEC-D) and in vivo, using chick embryo chorioallantoic membrane assay (CAM assay). Our in vitro results showed that obtustatin in comparison with MLO venom did not exhibit cytotoxic activity in HMVEC-D cells in comparison to MLO venom. But in vivo results have shown that 4µg /embryo (90 µM) of obtustatin inhibited angiogenesis induced by FGF2 by 17% while MLO snake venom induced 22% reduction of the angiogenic index. The concentration of obtustatin in the crude MLO venom was 0.3 nM, which is 300.000 times less than the concentration of the obtustatin itself. Given this enormous difference in concentration, it is likely that some components of the crude venom contribute to the observed anti-angiogenic effect. Hypotheses will be ascertained to justify this action: components in the MLO venom may increase obtustatin efficacy or have independent but synergic anti-angiogenic activities. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=angiogenesis" title="angiogenesis">angiogenesis</a>, <a href="https://publications.waset.org/abstracts/search?q=alpa1%20beta%201%20integrin" title=" alpa1 beta 1 integrin"> alpa1 beta 1 integrin</a>, <a href="https://publications.waset.org/abstracts/search?q=Macrovipera%20lebetina%20obtusa" title=" Macrovipera lebetina obtusa"> Macrovipera lebetina obtusa</a>, <a href="https://publications.waset.org/abstracts/search?q=obtustatin" title=" obtustatin"> obtustatin</a> </p> <a href="https://publications.waset.org/abstracts/85110/anti-angiogenic-effects-of-the-macrovipera-lebetina-obtusa-snake-crude-venom-and-obtustatin" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/85110.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">196</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">13</span> Isolation, Purification and Characterisation of Non-Digestible Oligosaccharides Derived from Extracellular Polysaccharide of Antarctic Fungus Thelebolus Sp. IITKGP-BT12 </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Abinaya%20Balasubramanian">Abinaya Balasubramanian</a>, <a href="https://publications.waset.org/abstracts/search?q=Satyabrata%20Ghosh"> Satyabrata Ghosh</a>, <a href="https://publications.waset.org/abstracts/search?q=Satyahari%20Dey"> Satyahari Dey</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Non-Digestible Oligosaccharides(NDOs) are low molecular weight carbohydrates with degree of polymerization (DP) 3-20, that are delivered intact to the large intestine. NDOs are gaining attention as effective prebiotic molecules that facilitate prevention and treatment of several chronic diseases. Recently, NDOs are being obtained by cleaving complex polysaccharides as it results in high yield and also as the former tend to display greater bioactivity. Thelebolus sp. IITKGP BT-12, a recently identified psychrophilic, Ascomycetes fungus has been reported to produce a bioactive extracellular polysaccharide(EPS). The EPS has been proved to possess strong prebiotic activity and anti- proliferative effects. The current study is an attempt to identify and optimise the most suitable method for hydrolysis of the above mentioned novel EPS into NDOs, and further purify and characterise the same. Among physical, chemical and enzymatic methods, enzymatic hydrolysis was identified as the best method and the optimum hydrolysis conditions obtained using response surface methodology were: reaction time of 24h, β-(1,3) endo-glucanase concentration of 0.53U and substrate concentration of 10 mg/ml. The NDOs were purified using gel filtration chromatography and their molecular weights were determined using MALDI-TOF. The major fraction was found to have a DP of 7,8. The monomeric units of the NDOs were confirmed to be glucose using TLC and GCMS-MS analysis. The obtained oligosaccharides proved to be non-digestible when subjected to gastric acidity, salivary and pancreatic amylases and hence could serve as efficient prebiotics. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=characterisation" title="characterisation">characterisation</a>, <a href="https://publications.waset.org/abstracts/search?q=enzymatic%20hydrolysis" title=" enzymatic hydrolysis"> enzymatic hydrolysis</a>, <a href="https://publications.waset.org/abstracts/search?q=non-digestible%20oligosaccharides" title=" non-digestible oligosaccharides"> non-digestible oligosaccharides</a>, <a href="https://publications.waset.org/abstracts/search?q=response%20surface%20methodology" title=" response surface methodology"> response surface methodology</a> </p> <a href="https://publications.waset.org/abstracts/104989/isolation-purification-and-characterisation-of-non-digestible-oligosaccharides-derived-from-extracellular-polysaccharide-of-antarctic-fungus-thelebolus-sp-iitkgp-bt12" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/104989.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">129</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">12</span> Mitigating the Aggregation of Human Islet Amyloid Polypeptide with Nanomaterials</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ava%20Faridi">Ava Faridi</a>, <a href="https://publications.waset.org/abstracts/search?q=Pouya%20Faridi"> Pouya Faridi</a>, <a href="https://publications.waset.org/abstracts/search?q=Aleksandr%20Kakinen"> Aleksandr Kakinen</a>, <a href="https://publications.waset.org/abstracts/search?q=Ibrahim%20Javed"> Ibrahim Javed</a>, <a href="https://publications.waset.org/abstracts/search?q=Thomas%20P.%20Davis"> Thomas P. Davis</a>, <a href="https://publications.waset.org/abstracts/search?q=Pu%20Chun%20Ke"> Pu Chun Ke</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Human islet amyloid polypeptide (IAPP) is a hormone associated with glycemic control and type 2 diabetes. Biophysically, the chirality of IAPP fibrils has been little explored with respect to the aggregation and toxicity of the peptide. Biochemically, it remains unclear as for how protein expression in pancreatic beta cells may be altered by cell exposure to the peptide, and how such changes may be mitigated by nanoparticle inhibitors for IAPP aggregation. In this study, we first demonstrated the elimination of the IAPP nucleation phase and shortening of its elongation phase by silica nanoribbons. This accelerated IAPP fibrillization translated to reduced toxicity, especially for the right-handed silica nanoribbons, as revealed by cell viability, helium ion microscopy, as well as zebrafish embryo survival, developmental and behavioral assays. We then examined the proteomes of βTC6 pancreatic beta cells exposed to the three main aggregation states of monomeric, oligomeric and amyloid fibrillar IAPP, and compared that with cellular protein expression modulated by graphene quantum dots (GQDs). A total of 29 proteins were significantly regulated by different forms of IAPP, and the majority of these proteins were nucleotide-binding proteins. A regulatory capacity of GQDs against aberrant protein expression was confirmed. These studies have demonstrated the great potential of employing nanomaterials targeting the mesoscopic enantioselectivity and protein expression dysregulation in pancreatic beta cells. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=graphene%20quantum%20dots" title="graphene quantum dots">graphene quantum dots</a>, <a href="https://publications.waset.org/abstracts/search?q=IAPP" title=" IAPP"> IAPP</a>, <a href="https://publications.waset.org/abstracts/search?q=silica%20nanoribbons" title=" silica nanoribbons"> silica nanoribbons</a>, <a href="https://publications.waset.org/abstracts/search?q=protein%20expression" title=" protein expression"> protein expression</a>, <a href="https://publications.waset.org/abstracts/search?q=toxicity" title=" toxicity"> toxicity</a> </p> <a href="https://publications.waset.org/abstracts/107515/mitigating-the-aggregation-of-human-islet-amyloid-polypeptide-with-nanomaterials" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/107515.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">142</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">11</span> Bio-Guided of Active New Alkaloids from Alstonia Brassi Toxicity Antitumour Activity in Silico and Molecular Modeling</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mesbah%20Khaled">Mesbah Khaled</a>, <a href="https://publications.waset.org/abstracts/search?q=Bouraoui%20Ouissal"> Bouraoui Ouissal</a>, <a href="https://publications.waset.org/abstracts/search?q=Benkiniouar%20Rachid"> Benkiniouar Rachid</a>, <a href="https://publications.waset.org/abstracts/search?q=Belkhiri%20Lotfi"> Belkhiri Lotfi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Alstonia, which are tropical plants with a wide geographical distribution, have been divided into different sections by different authors based on previous studies of several species within the genus. Monachino divides Alstonia into 5 sections, while Pichon divides it into 3 sections. Several plants belonging to this genus, such as Alstonia brassii, have been used in traditional folk medicine to treat ailments such as fever, malaria and dysentery]. Previous studies focusing on the chemical composition of these plants have successfully identified indol alkaloids with cytotoxic, anti-diabetic and anti-inflammatory properties. The newly discovered monomers are structurally similar to the backbones of picralin, affinisin and macrolin. On the other hand, all recently isolated dimeric compounds have a macrolin moiety. In this study, a computational analysis was performed on a series of novel molecules, including both monomeric and dimeric compounds with different structural frameworks. This investigation represents the first computational study of these molecules using an in silico approach incorporating 2D-QSAR data. The analysis involved various computational techniques, including 2D-QSAR modelling, molecular docking studies and subsequent validation by molecular dynamics simulation and assessment of ADMET properties. The chemical composition was identified by 1D and 2D NMR. Eight new alkaloids were isolated, 5 monomers and 3 dimers. In this section, we focus on the biological activity of 4 new alkaloids belonging to two different skeletons, the affinisine skeleton. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=affinisine" title="affinisine">affinisine</a>, <a href="https://publications.waset.org/abstracts/search?q=talcarpine" title=" talcarpine"> talcarpine</a>, <a href="https://publications.waset.org/abstracts/search?q=macroline" title=" macroline"> macroline</a>, <a href="https://publications.waset.org/abstracts/search?q=cytotoxicity" title=" cytotoxicity"> cytotoxicity</a>, <a href="https://publications.waset.org/abstracts/search?q=alkaloids" title=" alkaloids"> alkaloids</a> </p> <a href="https://publications.waset.org/abstracts/174842/bio-guided-of-active-new-alkaloids-from-alstonia-brassi-toxicity-antitumour-activity-in-silico-and-molecular-modeling" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/174842.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">351</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10</span> Preparation of Metal Containing Epoxy Polymer and Investigation of Their Properties as Fluorescent Probe</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ertu%C4%9F%20Y%C4%B1ld%C4%B1r%C4%B1m">Ertuğ Yıldırım</a>, <a href="https://publications.waset.org/abstracts/search?q=Dile%20Kara"> Dile Kara</a>, <a href="https://publications.waset.org/abstracts/search?q=Salih%20Zeki%20Y%C4%B1ld%C4%B1z"> Salih Zeki Yıldız </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Metal containing polymers (MCPs) are macro molecules usually containing metal-ligand coordination units and are a multidisciplinary research field mainly based at the interface between coordination chemistry and polymer science. The progress of this area has also been reinforced by the growth of several other closely related disciplines including macro molecular engineering, crystal engineering, organic synthesis, supra molecular chemistry and colloidal and material science. Schiff base ligands are very effective in constructing supra molecular architectures such as coordination polymers, double helical and triple helical complexes. In addition, Schiff base derivatives incorporating a fluorescent moiety are appealing tools for optical sensing of metal ions. MCPs are well-known systems in which the combinations of local parameters are possible by means of fluoro metric techniques. Generally, without incorporation of the fluorescent groups with polymers is unspecific, and it is not useful to analyze their fluorescent properties. Therefore, it is necessary to prepare a new type epoxy polymers with fluorescent groups in terms of metal sensing prop and the other photo chemical applications. In the present study metal containing polymers were prepared via poly functional monomeric Schiff base metal chelate complexes in the presence of dis functional monomers such as diglycidyl ether Bisphenol A (DGEBA). The synthesized complexes and polymers were characterized by FTIR, UV-VIS and mass spectroscopies. The preparations of epoxy polymers have been carried out at 185 °C. The prepared composites having sharp and narrow excitation/emission properties are expected to be applicable in various systems such as heat-resistant polymers and photo voltaic devices. The prepared composite is also ideal for various applications, easily prepared, safe, and maintain good fluorescence properties. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Schiff%20base%20ligands" title="Schiff base ligands">Schiff base ligands</a>, <a href="https://publications.waset.org/abstracts/search?q=crystal%20engineering" title=" crystal engineering"> crystal engineering</a>, <a href="https://publications.waset.org/abstracts/search?q=fluorescence%20properties" title=" fluorescence properties"> fluorescence properties</a>, <a href="https://publications.waset.org/abstracts/search?q=Metal%20Containing%20Polymers%20%28MCPs%29" title=" Metal Containing Polymers (MCPs)"> Metal Containing Polymers (MCPs)</a> </p> <a href="https://publications.waset.org/abstracts/17655/preparation-of-metal-containing-epoxy-polymer-and-investigation-of-their-properties-as-fluorescent-probe" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/17655.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">347</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">9</span> An Enzyme Technology - Metnin™ - Enables the Full Replacement of Fossil-Based Polymers by Lignin in Polymeric Composites</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Joana%20Antunes">Joana Antunes</a>, <a href="https://publications.waset.org/abstracts/search?q=Thomas%20Lev%C3%A9e"> Thomas Levée</a>, <a href="https://publications.waset.org/abstracts/search?q=Barbara%20Radovani"> Barbara Radovani</a>, <a href="https://publications.waset.org/abstracts/search?q=Anu%20Suonp%C3%A4%C3%A4"> Anu Suonpää</a>, <a href="https://publications.waset.org/abstracts/search?q=Paulina%20Saloranta"> Paulina Saloranta</a>, <a href="https://publications.waset.org/abstracts/search?q=Liji%20Sobhana"> Liji Sobhana</a>, <a href="https://publications.waset.org/abstracts/search?q=Petri%20Ihalainen"> Petri Ihalainen</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Lignin is an important component in the exploitation of lignocellulosic biomass. It has been shown that within the next years, the yield of added-value lignin-based chemicals and materials will generate renewable alternatives to oil-based products (e.g. polymeric composites, resins and adhesives) and enhance the economic feasibility of biorefineries. In this paper, a novel technology for lignin valorisation (METNIN™) is presented. METNIN™ is based on the oxidative action of an alkaliphilic enzyme in aqueous alkaline conditions (pH 10-11) at mild temperature (40-50 °C) combined with a cascading membrane operation, yielding a collection of lignin fractions (from oligomeric down to mixture of tri-, di- and monomeric units) with distinct molecular weight distribution, low polydispersity and favourable physicochemical properties. The alkaline process conditions ensure the high processibility of crude lignin in an aqueous environment and the efficiency of the enzyme, yielding better compatibility of lignin towards targeted applications. The application of a selected lignin fraction produced by METNIN™ as a suitable lignopolyol to completely replace a commercial polyol in polyurethane rigid foam formulations is presented as a prototype. Liquid lignopolyols with a high lignin content were prepared by oxypropylation and their full utilization in the polyurethane rigid foam formulation was successfully demonstrated. Moreover, selected technical specifications of different foam demonstrators were determined, including closed cell count, water uptake and compression characteristics. These specifications are within industrial standards for rigid foam applications. The lignin loading in the lignopolyol was a major factor determining the properties of the foam. In addition to polyurethane foam demonstrators, other examples of lignin-based products related to resins and sizing applications will be presented. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=enzyme" title="enzyme">enzyme</a>, <a href="https://publications.waset.org/abstracts/search?q=lignin%20valorisation" title=" lignin valorisation"> lignin valorisation</a>, <a href="https://publications.waset.org/abstracts/search?q=polyol" title=" polyol"> polyol</a>, <a href="https://publications.waset.org/abstracts/search?q=polyurethane%20foam" title=" polyurethane foam"> polyurethane foam</a> </p> <a href="https://publications.waset.org/abstracts/119479/an-enzyme-technology-metnin-enables-the-full-replacement-of-fossil-based-polymers-by-lignin-in-polymeric-composites" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/119479.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">152</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8</span> Bioethanol Production from Marine Algae Ulva Lactuca and Sargassum Swartzii: Saccharification and Process Optimization </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20Jerold">M. Jerold</a>, <a href="https://publications.waset.org/abstracts/search?q=V.%20Sivasubramanian"> V. Sivasubramanian</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20George"> A. George</a>, <a href="https://publications.waset.org/abstracts/search?q=B.S.%20Ashik"> B.S. Ashik</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20S.%20Kumar"> S. S. Kumar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Bioethanol is a sustainable biofuel that can be used alternative to fossil fuels. Today, third generation (3G) biofuel is gaining more attention than first and second-generation biofuel. The more lignin content in the lignocellulosic biomass is the major drawback of second generation biofuels. Algae are the renewable feedstock used in the third generation biofuel production. Algae contain a large number of carbohydrates, therefore it can be used for the fermentation by hydrolysis process. There are two groups of Algae, such as micro and macroalgae. In the present investigation, Macroalgae was chosen as raw material for the production of bioethanol. Two marine algae viz. Ulva Lactuca and Sargassum swartzii were used for the experimental studies. The algal biomass was characterized using various analytical techniques like Elemental Analysis, Scanning Electron Microscopy Analysis and Fourier Transform Infrared Spectroscopy to understand the physio-Chemical characteristics. The batch experiment was done to study the hydrolysis and operation parameters such as pH, agitation, fermentation time, inoculum size. The saccharification was done with acid and alkali treatment. The experimental results showed that NaOH treatment was shown to enhance the bioethanol. From the hydrolysis study, it was found that 0.5 M Alkali treatment would serve as optimum concentration for the saccharification of polysaccharide sugar to monomeric sugar. The maximum yield of bioethanol was attained at a fermentation time of 9 days. The inoculum volume of 1mL was found to be lowest for the ethanol fermentation. The agitation studies show that the fermentation was higher during the process. The percentage yield of bioethanol was found to be 22.752% and 14.23 %. The elemental analysis showed that S. swartzii contains a higher carbon source. The results confirmed hydrolysis was not completed to recover the sugar from biomass. The specific gravity of ethanol was found to 0.8047 and 0.808 for Ulva Lactuca and Sargassum swartzii, respectively. The purity of bioethanol also studied and found to be 92.55 %. Therefore, marine algae can be used as a most promising renewable feedstock for the production of bioethanol. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=algae" title="algae">algae</a>, <a href="https://publications.waset.org/abstracts/search?q=biomass" title=" biomass"> biomass</a>, <a href="https://publications.waset.org/abstracts/search?q=bioethaol" title=" bioethaol"> bioethaol</a>, <a href="https://publications.waset.org/abstracts/search?q=biofuel" title=" biofuel"> biofuel</a>, <a href="https://publications.waset.org/abstracts/search?q=pretreatment" title=" pretreatment"> pretreatment</a> </p> <a href="https://publications.waset.org/abstracts/115391/bioethanol-production-from-marine-algae-ulva-lactuca-and-sargassum-swartzii-saccharification-and-process-optimization" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/115391.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">159</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7</span> Assesment of Genetic Fidelity of Micro-Clones of an Aromatic Medicinal Plant Murraya koenigii (L.) Spreng</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ramesh%20Joshi">Ramesh Joshi</a>, <a href="https://publications.waset.org/abstracts/search?q=Nisha%20Khatik">Nisha Khatik</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Murraya koenigii (L.) Spreng locally known as “Curry patta” or “Meetha neem” belonging to the family Rutaceae that grows wildly in Southern Asia. Its aromatic leaves are commonly used as the raw material for traditional medicinal formulations in India. The leaves contain essential oil and also used as a condiment. Several monomeric and binary carbazol alkaloids present in the various plant parts. These alkaloids have been reported to possess anti-microbial, mosquitocidal, topo-isomerase inhibition and antioxidant properties. Some of the alkaloids reported in this plant have showed anti carcinogenic and anti-diabetic properties. The conventional method of propagation of this tree is limited to seeds only, which retain their viability for only a short period. Hence, a biotechnological approach might have an advantage edging over traditional breeding as well as the genetic improvement of M. koenigii within a short period. The development of a reproducible regeneration protocol is the prerequisite for ex situ conservation and micropropagation. An efficient protocol for high frequency regeneration of in vitro plants of Murraya koenigii via different explants such as- nodal segments, intermodal segments, leaf, root segments, hypocotyle, cotyledons and cotyledonary node explants is described. In the present investigation, assessment of clonal fidelity in the micropropagated plantlets of Murraya koenigii was attempted using RAPD and ISSR markers at different pathways of plant tissue culture technique. About 20 ISSR and 40 RAPD primers were used for all the samples. Genomic DNA was extracted by CTAB method. ISSR primer were found to be more suitable as compared to RAPD for the analysis of clonal fidelity of M. koenigii. The amplifications however, were finally performed using RAPD, ISSR markers owing to their better performance in terms of generation of amplification products. In RAPD primer maximum 75% polymorphism was recorded in OPU-2 series which exhibited out of 04 scorable bands, three bands were polymorphic with a band range of size 600-1500 bp. In ISSR primers the UBC 857 showed 50% polymorphism with 02 band were polymorphic of band range size between 400-1000 bp. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=genetic%20fidelity" title="genetic fidelity">genetic fidelity</a>, <a href="https://publications.waset.org/abstracts/search?q=Murraya%20koenigii" title=" Murraya koenigii"> Murraya koenigii</a>, <a href="https://publications.waset.org/abstracts/search?q=aromatic%20plants" title=" aromatic plants"> aromatic plants</a>, <a href="https://publications.waset.org/abstracts/search?q=ISSR%20primers" title=" ISSR primers "> ISSR primers </a> </p> <a href="https://publications.waset.org/abstracts/20710/assesment-of-genetic-fidelity-of-micro-clones-of-an-aromatic-medicinal-plant-murraya-koenigii-l-spreng" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/20710.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">501</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> Systematic Study of Structure Property Relationship in Highly Crosslinked Elastomers</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Natarajan%20Ramasamy">Natarajan Ramasamy</a>, <a href="https://publications.waset.org/abstracts/search?q=Gurulingamurthy%20Haralur"> Gurulingamurthy Haralur</a>, <a href="https://publications.waset.org/abstracts/search?q=Ramesh%20Nivarthu"> Ramesh Nivarthu</a>, <a href="https://publications.waset.org/abstracts/search?q=Nikhil%20Kumar%20Singha"> Nikhil Kumar Singha</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Elastomers are polymeric materials with varied backbone architectures ranging from linear to dendrimeric structures and wide varieties of monomeric repeat units. These elastomers show strongly viscous and weakly elastic when it is not cross-linked. But when crosslinked, based on the extent the properties of these elastomers can range from highly flexible to highly stiff nature. Lightly cross-linked systems are well studied and reported. Understanding the nature of highly cross-linked rubber based upon chemical structure and architecture is critical for varieties of applications. One of the critical parameters is cross-link density. In the current work, we have studied the highly cross-linked state of linear, lightly branched to star-shaped branched elastomers and determined the cross-linked density by using different models. Change in hardness, shift in Tg, change in modulus and swelling behavior were measured experimentally as a function of the extent of curing. These properties were analyzed using varied models to determine cross-link density. We used hardness measurements to examine cure time. Hardness to the extent of curing relationship is determined. It is well known that micromechanical transitions like Tg and storage modulus are related to the extent of crosslinking. The Tg of the elastomer in different crosslinked state was determined by DMA, and based on plateau modulus the crosslink density is estimated by using Nielsen’s model. Usually for lightly crosslinked systems, based on equilibrium swelling ratio in solvent the cross link density is estimated by using Flory–Rhener model. When it comes to highly crosslinked system, Flory-Rhener model is not valid because of smaller chain length. So models based on the assumption of polymer as a Non-Gaussian chain like 1) Helmis–Heinrich–Straube (HHS) model, 2) Gloria M.gusler and Yoram Cohen Model, 3) Barbara D. Barr-Howell and Nikolaos A. Peppas model is used for estimating crosslink density. In this work, correction factors are determined to the existing models and based upon it structure-property relationship of highly crosslinked elastomers was studied. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=dynamic%20mechanical%20analysis" title="dynamic mechanical analysis">dynamic mechanical analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=glass%20transition%20temperature" title=" glass transition temperature"> glass transition temperature</a>, <a href="https://publications.waset.org/abstracts/search?q=parts%20per%20hundred%20grams%20of%20rubber" title=" parts per hundred grams of rubber"> parts per hundred grams of rubber</a>, <a href="https://publications.waset.org/abstracts/search?q=crosslink%20density" title=" crosslink density"> crosslink density</a>, <a href="https://publications.waset.org/abstracts/search?q=number%20of%20networks%20per%20unit%20volume%20of%20elastomer" title=" number of networks per unit volume of elastomer"> number of networks per unit volume of elastomer</a> </p> <a href="https://publications.waset.org/abstracts/87235/systematic-study-of-structure-property-relationship-in-highly-crosslinked-elastomers" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/87235.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">165</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> Effect of Hypoxia on AOX2 Expression in Chlamydomonas reinhardtii</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Maria%20Ostroukhova">Maria Ostroukhova</a>, <a href="https://publications.waset.org/abstracts/search?q=Zhanneta%20Zalutskaya"> Zhanneta Zalutskaya</a>, <a href="https://publications.waset.org/abstracts/search?q=Elena%20Ermilova"> Elena Ermilova</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The alternative oxidase (AOX) mediates cyanide-resistant respiration, which bypasses proton-pumping complexes III and IV of the cytochrome pathway to directly transfer electrons from reduced ubiquinone to molecular oxygen. In Chlamydomonas reinhardtii, AOX is a monomeric protein that is encoded by two genes of discrete subfamilies, AOX1 and AOX2. Although AOX has been proposed to play essential roles in stress tolerance of organisms, the role of subfamily AOX2 is largely unknown. In C. reinhardtii, AOX2 was initially identified as one of constitutively low expressed genes. Like other photosynthetic organisms C. reinhardtii cells frequently experience periods of hypoxia. To examine AOX2 transcriptional regulation and role of AOX2 in hypoxia adaptation, real-time PCR analysis and artificial microRNA method were employed. Two experimental approaches have been used to induce the anoxic conditions: dark-anaerobic and light-anaerobic conditions. C. reinhardtii cells exposed to the oxygen deprivation have shown increased AOX2 mRNA levels. By contrast, AOX1 was not an anoxia-responsive gene. In C. reinhardtii, a subset of genes is regulated by transcription factor CRR1 in anaerobic conditions. Notable, the AOX2 promoter region contains the potential motif for CRR1 binding. Therefore, the role of CRR1 in the control of AOX2 transcription was tested. The CRR1-underexpressing strains, that were generated and characterized in this work, exhibited low levels of AOX2 transcripts under anoxic conditions. However, the transformants still slightly induced AOX2 gene expression in the darkness. These confirmed our suggestions that darkness is a regulatory stimulus for AOX genes in C. reinhardtii. Thus, other factors must contribute to AOX2 promoter activity under dark-anoxic conditions. Moreover, knock-down of CRR1 caused a complete reduction of AOX2 expression under light-anoxic conditions. These results indicate that (1) CRR1 is required for AOX2 expression during hypoxia, and (2) AOX2 gene is regulated by CRR1 together with yet-unknown regulatory factor(s). In addition, the AOX2-underexpressing strains were generated. The analysis of amiRNA-AOX2 strains suggested a role of this alternative oxidase in hypoxia adaptation of the alga. In conclusion, the results reported here show that C. reinhardtii AOX2 gene is stress inducible. CRR1 transcriptional factor is involved in the regulation of the AOX2 gene expression in the absence of oxygen. Moreover, AOX2 but not AOX1 functions under oxygen deprivation. This work was supported by Russian Science Foundation (research grant № 16-14-10004). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=alternative%20oxidase%202" title="alternative oxidase 2">alternative oxidase 2</a>, <a href="https://publications.waset.org/abstracts/search?q=artificial%20microRNA%20approach" title=" artificial microRNA approach"> artificial microRNA approach</a>, <a href="https://publications.waset.org/abstracts/search?q=chlamydomonas%20reinhardtii" title=" chlamydomonas reinhardtii"> chlamydomonas reinhardtii</a>, <a href="https://publications.waset.org/abstracts/search?q=hypoxia" title=" hypoxia"> hypoxia</a> </p> <a href="https://publications.waset.org/abstracts/60652/effect-of-hypoxia-on-aox2-expression-in-chlamydomonas-reinhardtii" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/60652.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span 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