AAV Packaging Service | GENEWIZ from Azenta

<!DOCTYPE html>    <html lang="en">  <head> <title>AAV Packaging Service | GENEWIZ from Azenta</title>  <meta charset="utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1"> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <meta name="description" content="AAV Packaging services center around GENEWIZ from Azenta’s proprietary AAV plasmid synthesis process to provide industry-leading project quality, completion time, and flexibility."> <meta name="keywords" content="aav packaging service, aav packaging, aav packaging services, aav package, aav packaging capacity, aav packaging limit, genewiz, azenta life sciences"> <meta name="author" content="">  <link rel="shortcut icon" href="favicon.png">   <link rel="stylesheet" href="/assets/plugins/bootstrap/css/bootstrap.min.css"> <link rel="stylesheet" href="/assets/css/style.css?v=1"> <link rel="stylesheet" href="/assets/css/custom.css?v=1"> <link rel="canonical" href="https://www.genewiz.com/Public/Services/Gene-Synthesis/AAV-Packaging/" /> <link rel="alternate" hreflang="x-default" href="https://www.genewiz.com/Public/Services/Gene-Synthesis/AAV-Packaging" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/Public/Services/Gene-Synthesis/AAV-Packaging" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/en/Public/Services/Gene-Synthesis/AAV-Packaging" /> <link rel="alternate" hreflang="en-GB" href="https://www.genewiz.com/en-GB/Public/Services/Gene-Synthesis/AAV-Packaging" /> <script type="text/javascript" src="/sitecore/shell/controls/lib/prototype/prototype.js"></script> <script type="text/javascript" src="/assets/plugins/jquery/jquery.min.js"></script> <script type="text/javascript"> var jQuery = $; //if (window.location.search.indexOf("sc_mode=edit") > 0) { jQuery.noConflict(); //} </script> <script type="text/javascript" src="/assets/plugins/bootstrap/js/bootstrap.min.js"></script> <script type="text/javascript" src="/assets/plugins/back-to-top.js"></script> <script type="text/javascript" src="/assets/js/app.js"></script>   <script> (function (w, d, s, l, i) { w[l] = w[l] || []; w[l].push( { 'gtm.start': new Date().getTime(), event: 'gtm.js' } ); var f = d.getElementsByTagName(s)[0], j = d.createElement(s), dl = l != 'dataLayer' ? 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href='/en/Public/Services/Next-Generation-Sequencing/RNA-Seq' style='color:#585F69;font-size:14px'>RNA-Seq</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Proteomics' style='color:#585F69;font-size:14px'>Proteomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/High-Throughput-Gene-Expression-Screening' style='color:#585F69;font-size:14px'>High-Throughput Gene Expression Screening <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Single-Cell-Sequencing' style='color:#585F69;font-size:14px'>Single-Cell Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Exome-Sequencing' style='color:#585F69;font-size:14px'>Exome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Amplicon-Sequencing-Services' style='color:#585F69;font-size:14px'>Amplicon Sequencing Services</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Standalone-NGS-Solutions' style='color:#585F69;font-size:14px'>Sequencing Only<br></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Whole-Genome-Sequencing' style='color:#585F69;font-size:14px'>Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/High-Throughput-Genotyping' style='color:#585F69;font-size:14px'>CRISPR Validation</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Targeted-Resequencing-Panels' style='color:#585F69;font-size:14px'>Targeted Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Metagenomics-Solutions' style='color:#585F69;font-size:14px'>Metagenomics </a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Epigenomics' style='color:#585F69;font-size:14px'>Epigenomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Antibody-Discovery' style='color:#585F69;font-size:14px'>Antibody Discovery <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Immunogenomics' style='color:#585F69;font-size:14px'>Immunogenomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Digital-Spatial-Profiling' style='color:#585F69;font-size:14px'>Digital Spatial Profiling</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/AAV-Genome-Sequencing' style='color:#585F69;font-size:14px'>AAV Genome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Whole-Plasmid-Sequencing-Plasmid-EZ' style='color:#585F69;font-size:14px'>Whole Plasmid Sequencing: Plasmid-EZ <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Sanger-Sequencing' style='color:#826ec3;font-size:17px'>Sanger Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Sanger-EZ' style='color:#585F69;font-size:14px'>Sanger-EZ <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Sanger-Sequencing/AAV-ITR' style='color:#585F69;font-size:14px'>AAV-ITR Sanger Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Purified-Templates' style='color:#585F69;font-size:14px'>Purified Templates</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Difficult-Template-Sequencing' style='color:#585F69;font-size:14px'>Difficult Template Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Direct-Colony-Sequencing' style='color:#585F69;font-size:14px'>Direct Colony Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/PCR-Purification' style='color:#585F69;font-size:14px'>PCR Purification</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Primer-Walking' style='color:#585F69;font-size:14px'>Primer Walking</a></li><br><li><a class='GColor' href='/en/Public/Services/Molecular-Genetics' style='color:#826ec3;font-size:17px'>PCR + Sanger Services</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Bacterial-and-Fungal-Identification' style='color:#585F69;font-size:14px'>Bacterial and Fungal Identification</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Mutation-Analysis' style='color:#585F69;font-size:14px'>Mutation Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/SNP-Genotyping' style='color:#585F69;font-size:14px'>SNP Genotyping</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package' style='color:#585F69;font-size:14px'>PCR Plus Sequencing CRISPR Analysis Package</a></li><li><a href='/en/Public/Services/Molecular-Genetics/cDNA-Verification' style='color:#585F69;font-size:14px'>Confirmatory Sequencing & Cell Bank Characterization</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Yeast-Colony-Sequencing' style='color:#585F69;font-size:14px'>Yeast Colony Sequencing</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Fragment-Analysis' style='color:#585F69;font-size:14px'>Fragment Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Solutions' style='color:#585F69;font-size:14px'>Quantitative PCR & Digital PCR <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Molecular-Genetics/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>Nucleic Acid Extraction</a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Gene-Synthesis' style='color:#826ec3;font-size:17px'>DNA & RNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Synthesis' style='color:#585F69;font-size:14px'>Gene Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Antibody-DNA-Synthesis' style='color:#585F69;font-size:14px'>Antibody Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/AAV-Plasmid-Synthesis' style='color:#585F69;font-size:14px'>AAV Plasmid Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Fragments' style='color:#585F69;font-size:14px'>Gene Fragments</a></li><li><a href='/en/Public/Services/Gene-Synthesis/CRISPR-Construct-Synthesis' style='color:#585F69;font-size:14px'>CRISPR Construct Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Synthetic-DNA-Libraries' style='color:#585F69;font-size:14px'>Synthetic DNA Libraries</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Single-Stranded-DNA-Synthesis' style='color:#585F69;font-size:14px'>ssDNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/DNA-Cloning' style='color:#585F69;font-size:14px'>DNA Cloning</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Site-Directed-Mutagenesis' style='color:#585F69;font-size:14px'>Site-Directed Mutagenesis<br></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Viral-Package' style='color:#585F69;font-size:14px'>Viral Packaging <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Recombinant-Antibody-Production' style='color:#585F69;font-size:14px'>Recombinant Antibody Production <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Custom-mRNA-Synthesis' style='color:#585F69;font-size:14px'>Custom mRNA Synthesis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><br><li><a class='GColor' href='/en/Public/Services/Oligo' style='color:#826ec3;font-size:17px'>Oligo Synthesis Services</a></li><li><a href='/en/Public/Services/Oligo/Overnight-DNA-Oligos' style='color:#585F69;font-size:14px'>Overnight DNA Oligos</a></li><li><a href='/en/Public/Services/Oligo/Custom-DNA-and-RNA-Oligos' style='color:#585F69;font-size:14px'>Custom DNA and RNA Oligos</a></li><li><a href='/en/Public/Services/Oligo/Oligo-Retained-for-Sanger-Sequencing' style='color:#585F69;font-size:14px'>Oligo Retained for Sanger Sequencing</a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Plasmid-DNA-Prep' style='color:#826ec3;font-size:17px'>Plasmid DNA Preparation</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/Standard' style='color:#585F69;font-size:14px'>Standard Plasmid DNA Prep</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/Targeted-Yield' style='color:#585F69;font-size:14px'>Targeted Yield Plasmid DNA Prep</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/AAV-Plasmid-Preparation' style='color:#585F69;font-size:14px'>AAV Plasmid Preparation</a></li><br><li><a class='GColor' href='/en/Public/Services/Clinical-Services' style='color:#826ec3;font-size:17px'>PreClinical and Clinical Services</a></li><li><a href='/en/Public/Services/Clinical-Services/Clinical-Services-Overview' style='color:#585F69;font-size:14px'>Clinical Services</a></li><li><a href='/en/Public/Services/Clinical-Services/Microarray-Solutions' style='color:#585F69;font-size:14px'>Microarray Solutions <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Variant-Confirmation' style='color:#585F69;font-size:14px'>CLIA Variant Confirmation (PCR + Sanger)</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Sanger-Sequencing' style='color:#585F69;font-size:14px'>CLIA Sanger Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Whole-Exome-Sequencing' style='color:#585F69;font-size:14px'>CLIA Whole Exome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Whole-Genome-Sequencing' style='color:#585F69;font-size:14px'>CLIA Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Integration-Site-Analysis' style='color:#585F69;font-size:14px'>CLIA Integration Site Analysis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li></ul></div></div></div></li></ul></li><li class='dropdown'><a href='/en/Public/Research-Areas' data-toggle='dropdown' class='dropdown-toggle' style='color:white;height:52px;top:47px;padding-top:0;background-color:transparent;font-size:17px;font-weight:bold;'>RESEARCH AREAS</a><ul class='dropdown-menu'><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/AAV' style='font-size:17px'>AAV Services</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Agriculture-Food-Science' style='font-size:17px'>Agriculture and Food Science</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='https://web.azenta.com/cell-and-gene-therapy' style='font-size:17px'>Cell and Gene Therapy Solutions <br></a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Biomarkers' style='font-size:17px'>Biomarkers</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Cancer-Research' style='font-size:17px'>Cancer Research</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Infectious-Disease' style='font-size:17px'>Infectious Disease</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Synthetic-Biology-Biotechnology' style='font-size:17px'>Synthetic Biology</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Metagenomics' style='font-size:17px'>Metagenomics</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Genome-Editing-and-Engineering' 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href="https://www.genewiz.com/Public/Services/Gene-Synthesis/AAV-Plasmid-Synthesis" target="_blank">AAV Vector Production</a> process to provide industry-leading project quality, completion time, and flexibility. The adeno-associated virus (AAV) is a powerful vehicle for cell and gene therapy. However, working with AAV vectors can be challenging. Each construct contains two inverted terminal repeat (ITR) sequences which are notoriously unstable, but also critical to the replication and encapsidation of viral DNA. Our support for AAV-based research and therapeutic applications enables us to deliver results for any AAV challenge.</p> <p> </p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/gene-synth-inquiry?utm_source=aav-packaging" target="_blank"><button>Contact an Expert</button></a> </div> <div class="fg-content-box" id="fg-content-box-right"> <iframe width="560" height="315" src="https://www.youtube.com/embed/htK2pbUclH4" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture;"></iframe> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .question { width: 1170px; display: inline-block; } .question p { width: 90%; text-align: left; } .question ul { width: 90%; text-align: left; } @media screen and (max-width: 1170px) { .question { width: auto; } } </style> <div class="question"> <h2>How is the AAV genome packaged?</h2> <p>The AAV genome is packaged by using two copies of inverted terminal repeats (ITR) to flank the expression cassette and facilitate vector DNA replication as single-stranded DNA (ssDNA) and packaging into empty preformed capsids. Small rep proteins are needed to assist in the translocation of the AAV genome into the capsid. </p> <p> </p> <p>AAV genome packaging components are:</p> <ul> <li>Assembled empty capsids </li> <li>Completed replication of AAV genomes </li> <li>Rep proteins to translocate the AAV genome into the capsid</li> </ul> <p> </p> <h2>What is the packaging capacity for AAV?</h2> <p>Adeno associated viruses (AAVs) have a packaging capacity of roughly 4.7kb, which is approximately half the packaging limit of vectors from lentiviruses and adenoviruses.</p> <p> </p> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>Applications of AAV Vectors</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Therapeutic Development: </strong> Treat diseases by delivery of genetic material through cell or gene therapies such as CAR T-cell and gene replacement </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>CRISPR/Gene Editing:</strong> Engineer cell lines with delivery of CRISPR/Cas9 and other gene editing platforms </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .research { width: 1170px; display: inline-block; text-align: left; padding-top: 20px; padding-bottom: 20px; } .research img { max-width: 900px; width: 100%; height: auto; display: block; margin-left: auto; margin-right: auto; } @media screen and (max-width: 1170px) { .research { width: auto; } .research img { width: 95%; height: auto; } } </style> <div class="research"> <h2 style="text-align: center;"> AAV Packaging Workflow</h2> <p>With expertise in Sanger and NGS sequencing, gene synthesis, viral packaging, and more, GENEWIZ has the capabilities and flexibility to partner with you for research success at any stage of your workflow. This means confidence and reliability in your product quality with the convenience of a single team for consultation and service experience. Our total packaging workflow below connects the novel advantages of our research groups to bring you AAV success.</p> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .workflow-container { padding-top: 30px; background: #fffffff; } .workflow { text-align: left; width: 1170px; display: inline-block } .workflow-step { vertical-align: top; width: 24%; padding: 10px; margin-bottom: 20px; display: inline-block; } .workflow-step img { width: 120px; margin: 0 auto; margin-bottom: 20px; } .workflow-step h2 { width: 80%; text-align: center; } .workflow-step h3 { text-align: center; font-size: 18px; font-family: sans-serif; border-bottom: none; } .workflow-step p { width: 80%; margin: 0 auto; text-align: left; font-size:11px; } .workflow-step p1 { width: 80%; margin: 0 auto; text-align: left; font-size:15px; } .workflow-icon { text-align: center; } @media only screen and (max-width: 1170px) { .workflow-container { width: auto; } .workflow { text-align: left; width: auto; } .workflow-step { width: 100%; } } </style> <div class="workflow-container"> <div class="workflow"> <div class="workflow-step"> <div class="workflow-icon"> <img alt="AAV ITR Sequencing Icon" src="/-/media/12051-D_AAV-Packaging_Icon_AAV-ITR-Seq.ashx?la=en&hash=59B371B699D81FBB11769017A18576576B74DAC1"></div> <div class="workflow-icon"><strong> </strong> <p1><strong>1. AAV ITR Sequencing</strong></p1> <br> <p><strong><br> Preservation of ITR Integrity:</strong> <br> GENEWIZ breakthrough Sanger Sequencing <a rel="noopener noreferrer" href="https://www.genewiz.com/Public/Services/Sanger-Sequencing/AAV-ITR" target="_blank">and ITR correction process</a> reads and conserves difficult regions that are critical to AAV success</p> </div> </div> <div class="workflow-step"> <div class="workflow-icon"> <img alt="AAV Plasmid Synthesis Icon" src="/-/media/12051-D_AAV-Packaging_Icon_AAV-PlasmidSynthesis.ashx?la=en&hash=13AC028A9D730E70972B485D6AC6DB109FD3CF75"></div> <div class="workflow-icon"><strong> <p1>2. AAV Plasmid Synthesis</p1> </strong> <br> <p><strong><br> High Fidelity Gene Synthesis:</strong> <br> 99.9% reliability in project delivery rate from a team with over 20 years of <a rel="noopener noreferrer" href="https://www.genewiz.com/Public/Services/Gene-Synthesis/AAV-Plasmid-Synthesis" target="_blank">gene synthesis experience</a></p> </div> </div> <div class="workflow-step"> <div class="workflow-icon"> <img alt="Antibody-Icon" src="/-/media/12051-D_AAV-Packaging_Icon_AAV-PlasmidPrep.ashx?la=en&hash=A8DB390E8B0922A7F24782048A690DFBBDCEA423"></div> <div class="workflow-icon"><strong> <p1>3. AAV Plasmid Preparation</p1> </strong> <br> <p><strong><br> Scalable Viral Prep:</strong><br> Mini- to giga-scale quantities of AAV plasmids available from GENEWIZ <a rel="noopener noreferrer" href="https://www.genewiz.com/Public/Services/Plasmid-DNA-Prep/AAV-Plasmid-Preparation" target="_blank">proprietary <em>E. coli</em> strains</a> for optimal AAV performance</p> </div> </div> <div class="workflow-step"> <div class="workflow-icon"> <img alt="AAV Packaging Icon" src="/-/media/12051-D_AAV-Packaging_Icon_AAV-Packaging.ashx?la=en&hash=DF7F37BA1436D1DDFF2D6B9794F4B0F6D760AE64"></div> <div class="workflow-icon"><strong> <p1>4. AAV Packaging</p1> </strong> <br> <p><strong><br> AAV Packaging:</strong><br> Generate high-quality viral particles with customizable synthesis, serotype, and scale options</p> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .research { width: 1170px; display: inline-block; text-align: left; padding-top: 20px; padding-bottom: 20px; } .research img { max-width: 900px; width: 100%; height: auto; display: block; margin-left: auto; margin-right: auto; } @media screen and (max-width: 1170px) { .research { width: auto; } .research img { width: 95%; height: auto; } } </style> <div class="research"> <h2 style="text-align: center;"> Featured Application: AAV for Therapeutic Delivery</h2> <p>Adeno-associated virus (AAV) is a promising gene therapy vector due to its low pathogenicity and immunogenicity. It can deliver a genetic payload of up to 4.7 kb, and multiple serotypes are available to target specific cell types or tissues in humans. The recombinant AAV (rAAV) genome comprises a transgene construct flanked by a pair of 145 bp inverted terminal repeat (ITR) sequences. Previous reports have shown that the ITRs are necessary to produce viral particles but their structure is problematic for conventional cloning workflows. ITR sequences in plasmids are unstable, often acquiring deletions during propagation in bacteria. Because of the high risk of mutation, sequence verification of ITR sequences is a critical part of rAAV quality control. This too is challenging, however, as standard Sanger sequencing fails to read through most ITR sequences. Here, we study the effect of partial and full ITR deletions on AAV packaging and show how proprietary methods developed by GENEWIZ can successfully detect and repair these mutations.</p> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; } .banner { width: 1170px; background-image: url('/-/media/12051-D_AAV-Packaging_WebBanFull-Lrg_Background.ashx'); background-size: cover; text-align: center; display: flex; padding: 20px; } .banner img { text-align: center; display: block; margin-left: auto; margin-right: auto; } #banner-left { width: 30%; padding: 10px; } #banner-right { width: 69%; padding: 20px; } #banner-right h1 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 45px; margin: 0; margin-bottom: 15px; color: #FFFFFF } #banner-right h3 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 30px; margin: 0; margin-bottom: 15px; color: #FFFFFF } #banner-right button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #FFFFFF; border: 2px solid #FFFFFF; border-radius: 3px; transition: all .2s ease-in-out; font-size: 15px; font-weight: bold; } #banner-right button:hover { cursor: pointer; transform: scale(1.1); } #banner-right button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .banner { width: 95%; height: auto; background: #000000; display: inline-block; } #banner-left { text-align: center; width: 100%; } #banner-right { text-align: center; width: 100%; } #banner-right h1 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 45px; margin: 0; margin-bottom: 15px; color: #FFFFFF } #banner-right h3 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 30px; margin: 0; margin-bottom: 15px; color: #FFFFFF } #banner-left img { width: auto; height: auto; } #banner-right img { width: 90%; height: auto; } @media only screen and (max-width: 700px) { #banner-left img { width: 60%; } #banner-right button { font-size: 14px; } } </style> <div class="body"> <div class="banner"> <div id="banner-left"> <img alt="" src="/-/media/12051-D_AAV-Packaging_WebBanFull-Lrg_Thumbnail.ashx?la=en&hash=A3B6A0CB128447EE39930218FAF40BB514BD01C8" /> </div> <div id="banner-right"> <div id="banner-right h1"> <h3 style="color: #826ec3;">TECH NOTE</h3> <h1>An Efficient and Reliable<br /> Approach to AAV Packaging</h1> <div id="banner-right h3"> <br /> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/tech-note/aav-packaging" target="_blank"><button>DOWNLOAD</button></a> </div> </div> </div> </div> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>Features and Benefits</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>High-Quality Viral Particles</strong> – High purity, high yield, with low endotoxin and empty shell </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Rapid Project Completion</strong> – AAV packaging in as fast as 13-15 business days (BD) to meet your research deadlines </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Accurate ITR and Transgene Expression</strong> - The GENEWIZ proprietary AAV plasmid synthesis workflow ensures better ITR integrity than any other research partner </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Ph.D.–Level Partnership</strong> - Our dedicated project management teams provide expert technical consultation to solve any AAV challenge </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Reliable Project Management</strong> – Global production facilities and local project management ensures reliable progress and proactive communication </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Customized AAV Delivery</strong> – Suit the needs of your project with over 20 serotypes and a wide range of scales for AAV particles </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Total Cell and Gene Therapy Solutions</strong> – Protect your IP and reduce logistical delays and errors by working with GENEWIZ as your single partner for sequencing, synthesis, and sample management </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .research { width: 1170px; display: inline-block; text-align: left; padding-top: 20px; padding-bottom: 20px; } .research img { max-width: 900px; width: 100%; height: auto; display: block; margin-left: auto; margin-right: auto; } @media screen and (max-width: 1170px) { .research { width: auto; } .research img { width: 95%; height: auto; } } </style> <div class="research"> <h2 style="text-align: center;"> AAV Packaging Deliverables</h2> <p>AAV particles have a packaging capacity of approximately 4.7 kb and are provided in PBS buffer<sup>1</sup>. They are shipped on dry ice and aliquoted in custom vialing sizes for convenient storage and use.</p> <p> </p> <table class="table table-responsive table-bordered"> <tbody> <tr class="panel-heading"> <th style="text-align: center; background-color:#826ec3; color:#FFFFFF;" width="20%"><span style="font-weight: normal;"><strong>Featured AAV Serotypes<sup>2</sup></strong></span></th> <th style="text-align: center; background-color:#826ec3; color:#FFFFFF;" width="15%"><span style="font-weight: normal;"><strong>Volume</strong></span></th> <th style="text-align: center; background-color:#826ec3; color:#FFFFFF;" width="20%"><span style="font-weight: normal;"><strong>Estimated Completion Time</strong></span></th> <th style="text-align: center; background-color:#826ec3; color:#FFFFFF;" width="20%"><span style="font-weight: normal;"><strong>Quality</strong></span></th> <th style="text-align: center; background-color:#826ec3; color:#FFFFFF;" width="10%"><strong>Titer<sup>3</sup></strong></th> <th style="text-align: center; background-color:#826ec3; color:#FFFFFF;" width="15%"><strong>Yield</strong></th> </tr> <tr class="panel-heading"> <th style="text-align: left; color:#000;" rowspan="2"><span style="font-weight: normal;">Normal yield: 1, 3b, 5, 7, 8, 9, rh10, PHP.eB, PHP.B, AAVDJ, retro, PHP.S, Olig001, Olig001-PHPA, AAVie </span></th> <th style="text-align: center; color:#000; padding-top:10px;"><span style="font-weight: normal;">200 uL</span></th> <th style="text-align: center; color:#000;" rowspan="4"><span style="font-weight: normal;"> <br /> <br /> <br /> In as few as 3-4 weeks</span></th> <th style="text-align: center; color:#000;" rowspan="2"><span style="font-weight: normal;">- Ultrapure (≥95% by  SDS-PAGE)</span></th> <th style="text-align: left; color:#000;" rowspan="4"> <span style="font-weight: normal;"><br /> <br /> <br /> 1 x 10<sup>13</sup> GC/mL</span></th> <th style="text-align: left; color:#000;" rowspan="4"><span style="font-weight: normal;"><br /> <br /> <br /> Up to 1 x 10<sup>16</sup> GC</span></th> </tr> <tr class="panel-heading"> <th style="text-align: center; color:#000; padding-top:10px;"><span style="font-weight: normal;">500 uL</span></th> </tr> <tr class="panel-heading"> <th style="text-align: left; color:#000;" rowspan="2"><span style="font-weight: normal;">Low yield: 2, 4, 6, 6.2, 7m8, AAV ShH10, AAV6 [Y705F,Y731F]</span></th> <th style="text-align: center; color:#000; padding-top:10px;"><span style="font-weight: normal;">1 mL</span></th> <th style="text-align: center; color:#000;" rowspan="2"><span style="font-weight: normal;">- Low endotoxin (<10 EU/mL) </span></th> </tr> <tr class="panel-heading"> <th style="text-align: center; color:#000; padding-top:10px;"><span style="font-weight: normal;">Custom (up to 100 mL)</span></th> </tr> </tbody> </table> <p>1. AAV packaging solutions can use GENEWIZ or customer-supplied plasmids.</p> <p>2. GENEWIZ is continually developing new serotypes through research efforts. <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/aav-packaging" target="_blank">Contact our project management team to learn more</a>.</p> <p>3. Standard quality control includes titer determination by qPCR with ITR primers. Optional dPCR and TEM analysis available.</p> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; } .banner3 { width: 1170px; background-image: url('/-/media/12054-D_Gene-DIscovery-Solutions_WebBanFull-Med_BG.ashx'); background-size: cover; text-align: left; display: flex; padding: 10px; } .banner3 img { text-align: center; display: block; margin-left: auto; margin-right: auto; } #banner3-left { width: 50%; padding: 20px; } #banner3-right { width: 49%; padding: 10px; } #banner3-right h2 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 0; margin-bottom: 5px; color: #FFFFFF } #banner3-left h2 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 5; margin-bottom: 0px; color: #FFFFFF 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