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KEGG ENZYME: 1.14.13.246
<!doctype html> <html><head> <title>KEGG ENZYME: 1.14.13.246</title> <link type="text/css" rel="stylesheet" href="/css/gn2.css?1732464181"> <link type="text/css" rel="stylesheet" href="/css/bget.css?1732464181"> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <link type="text/css" rel="stylesheet" href="/css/bgetm.css?1732464181" media="only screen and (max-width: 768px)"> <style>.kcfd{display:none;}</style> <script> <!-- var getElementsByClassName = document.getElementsByClassName ? function (cl){ return document.getElementsByClassName(cl); } : function (cl){ var elms = []; var objs = document.getElementsByTagName('*'); for(var i=0;i<objs.length;i++){ var obj = objs[i]; if(obj.className == cl){ elms.push(obj); } } return elms; }; function toggleField(clk,cl,show,hide){ var objs = getElementsByClassName(cl); for(var i=0;i<objs.length;i++){ var obj = objs[i]; if(!obj.style.display||obj.style.display == 'none'){ obj.style.display = 'block'; clk.innerHTML = ' « '+hide; 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} } } function Link_XtrctSeq2(form) { var dna_from; var dna_to; var dna_len; var plus_up = Number(form.XtrctSeq_UP.value) ; var plus_down = Number(form.XtrctSeq_DOWN.value); var vector = Number(form.VECTOR.value); var org = form.ORG.value; var chr = form.CHR.value; var kid = form.KEGGID.value; var url; if (plus_up == 0 && plus_down == 0) { url = "/entry/-f+-n+n+" + kid; } else { if (vector == 1) { dna_from = Number(form.FROM.value) - plus_up; dna_to = Number(form.TO.value) + plus_down; } else { dna_from = Number(form.FROM.value) - plus_down; dna_to = Number(form.TO.value) + plus_up; } url = "/dbget-bin/cut_sequence_genes.pl?FROM=" + dna_from + "&TO=" + dna_to +"&VECTOR=" + vector + "&ORG=" + org; if (chr) url += "&CHR=" + chr; } //window.open( url, "_self" ); location.href = url; } function go_taxonomy(form,params){ for(var key in params){ if(form[key]){ form.setAttribute(key,params[key]); } else{ var obj = document.createElement('input'); obj.setAttribute('type','hidden'); obj.setAttribute('name',key); obj.setAttribute('value',params[key]); form.appendChild(obj); } } form.submit(); } window.onload=function(){window.focus();init();}; //---> </script> </head> <body> <div> <table border=0 cellpadding=0 cellspacing=0><tr><td> <table border=0 cellpadding=0 cellspacing=0 width="683"><tr><td width=70><a href="/kegg/kegg2.html"><img align="middle" alt="KEGG" border=0 src="/Fig/bget/kegg2.gif"></a></td><td> </td><td><a name="enzyme:1.14.13.246"></a><font class="title2">ENZYME: 1.14.13.246</font></td><td class="tar vbot"><button class="btn" onclick="javascript:void(window.open('/kegg/document/help_bget_enzyme.html','KEGG_Help','toolbar=no,location=no,directories=no,width=720,height=640,resizable=yes,scrollbars=yes'));return false;">Help</button></td></tr></table><form method="post" action="/kegg-bin/search_htext" enctype="application/x-www-form-urlencoded" id="form1" name="form1"></form><table class="w1" width="650"> <tr> <td class="fr2 w1"> <table width="650" class="w2"> <tr><th class="th20 deft tal vmid"><span class="nowrap">Entry</span></th> <td class="td20 defd"><table class="w1" width="100%"><tr><td class="tal pd0"><code><span class="nowrap">EC 1.14.13.246 Enzyme <br> </span></code></td></tr></table></td></tr> <tr><th class="th21 deft tal vtop"><span class="nowrap">Name</span></th> <td class="td21 defd"><div class="cel"><div class="cel">4beta-methylsterol monooxygenase;<br> sdmA (gene name)<br> </div></div></td></tr> <tr><th class="th20 deft tal vtop"><span class="nowrap">Class</span></th> <td class="td20 defd"><div class="cel">Oxidoreductases;<br> Acting on paired donors, with incorporation or reduction of molecular oxygen;<br> With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor<br> <button class="btn" onclick="location.href='/brite/ko01000+1.14.13.246';return false;">BRITE hierarchy</button></div></td></tr> <tr><th class="th21 deft tal vtop"><span class="nowrap">Sysname</span></th> <td class="td21 defd"><div class="cel">3beta-hydroxy-4,4-dimethylsteroid,NADH:oxygen oxidoreductase (C-4mbeta-hydroxylating)<br> </div></td></tr> <tr><th class="th20 deft tal vtop"><span class="nowrap">Reaction(IUBMB)</span></th> <td class="td20 defd"><div class="cel">a 3beta-hydroxy-4,4-dimethylsteroid + 3 NADH + 3 H+ + 3 O2 = a 3beta-hydroxy-4alpha-methylsteroid-4beta-carboxylate + 3 NAD+ + 4 H2O (overall reaction) [RN:<a href="/entry/R12360">R12360</a>];<br> (1a) a 3beta-hydroxy-4,4-dimethylsteroid + NADH + H+ + O2 = a 3beta-hydroxy-4beta-hydroxymethyl-4alpha-methylsteroid + NAD+ + H2O [RN:<a href="/entry/R12357">R12357</a>];<br> (1b) a 3beta-hydroxy-4beta-hydroxymethyl-4alpha-methylsteroid + NADH + H+ + O2 = a 3beta-hydroxy-4beta-formyl-4alpha-methylsteroid + NAD+ + 2 H2O [RN:<a href="/entry/R12358">R12358</a>];<br> (1c) a 3beta-hydroxy-4beta-formyl-4alpha-methylsteroid + NADH + H+ + O2 = a 3beta-hydroxy-4alpha-methylsteroid-4beta-carboxylate + NAD+ + H2O [RN:<a href="/entry/R12359">R12359</a>]<br> </div></td></tr> <tr><th class="th21 deft tal vtop"><span class="nowrap">Reaction(KEGG)</span></th> <td class="td21 defd"><div class="cel"><a href="/entry/R12357">R12357</a> <a href="/entry/R12358">R12358</a> <a href="/entry/R12359">R12359</a> <a href="/entry/R12360">R12360</a><br> <button class="btn" onclick="location.href='/entry/R12357/R12358/R12359/R12360';return false;">Reaction</button></div></td></tr> <tr><th class="th20 deft tal vtop"><span class="nowrap">Substrate</span></th> <td class="td20 defd"><div class="cel">3beta-hydroxy-4,4-dimethylsteroid [CPD:<a href="/entry/C22103">C22103</a>];<br> NADH [CPD:<a href="/entry/C00004">C00004</a>];<br> H+ [CPD:<a href="/entry/C00080">C00080</a>];<br> O2 [CPD:<a href="/entry/C00007">C00007</a>];<br> 3beta-hydroxy-4beta-hydroxymethyl-4alpha-methylsteroid [CPD:<a href="/entry/C22104">C22104</a>];<br> 3beta-hydroxy-4beta-formyl-4alpha-methylsteroid [CPD:<a href="/entry/C22105">C22105</a>]<br> </div></td></tr> <tr><th class="th21 deft tal vtop"><span class="nowrap">Product</span></th> <td class="td21 defd"><div class="cel"><div class="cel">3beta-hydroxy-4alpha-methylsteroid-4beta-carboxylate [CPD:<a href="/entry/C22106">C22106</a>];<br> NAD+ [CPD:<a href="/entry/C00003">C00003</a>];<br> H2O [CPD:<a href="/entry/C00001">C00001</a>];<br> 3beta-hydroxy-4beta-hydroxymethyl-4alpha-methylsteroid [CPD:<a href="/entry/C22104">C22104</a>];<br> 3beta-hydroxy-4beta-formyl-4alpha-methylsteroid [CPD:<a href="/entry/C22105">C22105</a>]<br> </div></div></td></tr> <tr><th class="th20 deft tal vtop"><span class="nowrap">Comment</span></th> <td class="td20 defd"><div class="cel">Contains a Rieske [2Fe-2S] iron-sulfur cluster. This bacterial enzyme (SdmA) participates in the biosynthesis of bacterial sterols. Together with SdmB it forms an enzyme system that removes one methyl group from the C-4 position of 4,4-dimethylated steroid molecules. SdmA catalyses three successive oxidations of the C-4beta methyl group, turning it into a carboxylate group; the second enzyme, SdmB, is a bifunctional enzyme that catalyses two different activities. As EC <a href="/entry/1.1.1.417">1.1.1.417</a>, 3beta-hydroxysteroid-4beta-carboxylate 3-dehydrogenase (decarboxylating), it catalyses an oxidative decarboxylation that results in reduction of the 3beta-hydroxy group at the C-3 carbon to an oxo group. As EC <a href="/entry/1.1.1.270">1.1.1.270</a>, 3beta-hydroxysteroid 3-dehydrogenase, it reduces the 3-oxo group back to a 3beta-hydroxyl. Unlike the animal/fungal enzyme EC <a href="/entry/1.14.18.9">1.14.18.9</a>, 4alpha-methylsterol monooxygenase, and the plant enzymes EC <a href="/entry/1.14.18.10">1.14.18.10</a>, plant 4,4-dimethylsterol C-4alpha-methyl-monooxygenase, and EC <a href="/entry/1.14.18.11">1.14.18.11</a>, plant 4alpha-monomethylsterol monooxygenase, this enzyme acts preferentially on the 4beta-methyl group. Since no epimerization of the remaining C-4alpha methyl group occurs, the enzyme can only remove one methyl group, leaving a 4alpha-monomethylated product. Known substrates include 4,4-dimethyl-5alpha-cholest-8-en-3beta-ol and 14-demethyllanosterol.<br> </div></td></tr> <tr><th class="th21 deft tal vtop"><span class="nowrap">History</span></th> <td class="td21 defd"><div class="cel">EC 1.14.13.246 created 2019<br> </div></td></tr> <tr><th class="th20 deft tal vtop"><span class="nowrap">Orthology</span></th> <td class="td20 defd"><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><a href="/entry/K23556">K23556</a> </span></td><td>4beta-methylsterol monooxygenase</td></tr></table></td></tr> <tr><th class="th21 deft tal vtop"><span class="nowrap">Genes</span></th> <td class="td21 defd"><style>.g1{display:none;}</style><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><a href="/dbget-bin/get_linkdb?-t+MCA+ec:1.14.13.246" target="_blank">MCA</a>: </span></td><td>MCA1016</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><a href="/dbget-bin/get_linkdb?-t+METU+ec:1.14.13.246" target="_blank">METU</a>: </span></td><td>GNH96_12925</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><a href="/dbget-bin/get_linkdb?-t+MMEO+ec:1.14.13.246" target="_blank">MMEO</a>: </span></td><td>OOT43_17980</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><a href="/dbget-bin/get_linkdb?-t+MMAI+ec:1.14.13.246" target="_blank">MMAI</a>: </span></td><td>sS8_3007</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><a href="/dbget-bin/get_linkdb?-t+MSZE+ec:1.14.13.246" target="_blank">MSZE</a>: </span></td><td>MSZNOR_4036</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><a href="/dbget-bin/get_linkdb?-t+MMOB+ec:1.14.13.246" target="_blank">MMOB</a>: </span></td><td>F6R98_01405</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><a href="/dbget-bin/get_linkdb?-t+MISZ+ec:1.14.13.246" target="_blank">MISZ</a>: </span></td><td>MishRS11D_00090</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><a href="/dbget-bin/get_linkdb?-t+MESL+ec:1.14.13.246" target="_blank">MESL</a>: </span></td><td>KKZ03_12725</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><a href="/dbget-bin/get_linkdb?-t+PAUU+ec:1.14.13.246" target="_blank">PAUU</a>: </span></td><td>E8A73_027885</td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><a href="/dbget-bin/get_linkdb?-t+MRM+ec:1.14.13.246" target="_blank">MRM</a>: </span></td><td>A7982_00877</td></tr></table><div class="g1"><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><a href="/dbget-bin/get_linkdb?-t+SAMY+ec:1.14.13.246" target="_blank">SAMY</a>: </span></td><td>DB32_006293</td></tr></table></div><div class="g1"><table class="w1"><tr><td class="vtop pd0"><span class="nowrap"><a href="/dbget-bin/get_linkdb?-t+NANN+ec:1.14.13.246" target="_blank">NANN</a>: </span></td><td>O0S08_14310</td></tr></table></div><a href="javascript:void(0)" onclick="javascript:toggleField(this,"g1","show all","hide")"> » show all</a><br> <input type="hidden" name="query" form="form1" value="MCA METU MMEO MMAI MSZE MMOB MISZ MESL PAUU MRM SAMY NANN"><input type="hidden" name="htext" form="form1" value="br08601.keg"><input type="hidden" name="option" form="form1" value="-a"><button class="btn" onclick="javascript:go_taxonomy(document.form1,{"action":"/kegg-bin/search_htext"});return false;">Taxonomy</button></td></tr> <tr><th class="th20 deft tal vtop"><span class="nowrap">Reference</span></th> <td class="td20 defd"><div class="cel">1 [PMID:<a href="https://www.ncbi.nlm.nih.gov/pubmed/29784781">29784781</a>]</div></td></tr> <tr><th class="th21 rept tal vtop"><span class="nowrap"> Authors</span></th> <td class="td21 repd"><div class="cel">Lee AK, Banta AB, Wei JH, Kiemle DJ, Feng J, Giner JL, Welander PV</div></td></tr> <tr><th class="th20 rept tal vtop"><span class="nowrap"> Title</span></th> <td class="td20 repd"><div class="cel">C-4 sterol demethylation enzymes distinguish bacterial and eukaryotic sterol synthesis.</div></td></tr> <tr><th class="th21 rept tal vtop"><span class="nowrap"> Journal</span></th> <td class="td21 repd"><div class="cel">Proc Natl Acad Sci U S A 115:5884-5889 (2018)<br>DOI:<a href="https://doi.org/10.1073/pnas.1802930115">10.1073/pnas.1802930115</a></div></td></tr> <tr><th class="th20 rept tal vtop"><span class="nowrap"> Sequence</span></th> <td class="td20 repd"><div class="cel">[mca:<a href="/entry/mca:MCA1016">MCA1016</a>]</div></td></tr> <tr><th class="th21 deft tal vtop"><span class="nowrap">Other DBs</span></th> <td class="td21 defd"><table class="w1"><tr><td class="vtop pd0"><span class="nowrap">ExplorEnz - The Enzyme Database: </span></td><td><a href="https://www.enzyme-database.org/query.php?ec=1.14.13.246">1.14.13.246</a></td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap">IUBMB Enzyme Nomenclature: </span></td><td><a href="https://iubmb.qmul.ac.uk/enzyme/EC1/14/13/246.html">1.14.13.246</a></td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap">ExPASy - ENZYME nomenclature database: </span></td><td><a href="https://enzyme.expasy.org/EC/1.14.13.246">1.14.13.246</a></td></tr></table><table class="w1"><tr><td class="vtop pd0"><span class="nowrap">BRENDA, the Enzyme Database: </span></td><td><a href="https://www.brenda-enzymes.info/enzyme.php?ecno=1.14.13.246">1.14.13.246</a></td></tr></table></td></tr> </table></td></tr></table> <br></td><td class="vtop"><div class="aw" style="margin-top:46px;"><div class="fm at th20"> All links </div> <div class="fm"><pre class="fm" style="margin-left:1em"> <span style="color:#006"><a href="/dbget-bin/get_linkdb?-t+6+ec:1.14.13.246">Chemical substance (9)</a></span> <a href="/dbget-bin/get_linkdb?-t+compound+ec:1.14.13.246">KEGG COMPOUND (9)</a> <span style="color:#006"><a href="/dbget-bin/get_linkdb?-t+7+ec:1.14.13.246">Chemical reaction (8)</a></span> <a href="/dbget-bin/get_linkdb?-t+reaction+ec:1.14.13.246">KEGG REACTION (4)</a> <a href="/dbget-bin/get_linkdb?-t+rclass+ec:1.14.13.246">KEGG RCLASS (4)</a> <span style="color:#006"><a href="/dbget-bin/get_linkdb?-t+9+ec:1.14.13.246">Gene (17)</a></span> <a href="/dbget-bin/get_linkdb?-t+orthology+ec:1.14.13.246">KEGG ORTHOLOGY (1)</a> <a href="/dbget-bin/get_linkdb?-t+genes+ec:1.14.13.246">KEGG GENES (12)</a> <a href="/dbget-bin/get_linkdb?-t+mgenes+ec:1.14.13.246">KEGG MGENES (4)</a> <span style="color:#006"><a href="/dbget-bin/get_linkdb?-t+10+ec:1.14.13.246">Protein sequence (4)</a></span> <a href="/dbget-bin/get_linkdb?-t+uniprot+ec:1.14.13.246">UniProt (3)</a> <a href="/dbget-bin/get_linkdb?-t+swissprot+ec:1.14.13.246">SWISS-PROT (1)</a> <span style="color:#006"><a href="/dbget-bin/get_linkdb?-t+11+ec:1.14.13.246">DNA sequence (6)</a></span> <a href="/dbget-bin/get_linkdb?-t+genbank+ec:1.14.13.246">GenBank (3)</a> <a href="/dbget-bin/get_linkdb?-t+embl+ec:1.14.13.246">EMBL (3)</a> <span style="color:#006"><a href="/dbget-bin/get_linkdb?-t+13+ec:1.14.13.246">Protein domain (4)</a></span> <a href="/dbget-bin/get_linkdb?-t+interpro+ec:1.14.13.246">InterPro (4)</a> <a href="/dbget-bin/get_linkdb?-t+alldb+ec:1.14.13.246">All databases (48)</a> <br><a href="/dbget-bin/get_linkdb?-N+ec:1.14.13.246">Download RDF</a> </pre> </div></div> </td></tr></table> <a href="/dbget/">DBGET</a> integrated database retrieval system</div> </body> </html>