Search results for: cytospins

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cytospins</title> <meta name="description" content="Search results for: cytospins"> <meta name="keywords" content="cytospins"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="cytospins"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 2</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: cytospins</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Immunocytochemical Stability of Antigens in Cytological Samples Stored in In-house Liquid-Based Medium</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Anamarija%20Kuhar">Anamarija Kuhar</a>, <a href="https://publications.waset.org/abstracts/search?q=Veronika%20Kloboves%20Prevodnik"> Veronika Kloboves Prevodnik</a>, <a href="https://publications.waset.org/abstracts/search?q=Nata%C5%A1a%20Nolde"> Nata拧a Nolde</a>, <a href="https://publications.waset.org/abstracts/search?q=Ulrika%20Klop%C4%8Di%C4%8D"> Ulrika Klop膷i膷</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The decision for immunocytochemistry (ICC) is usually made in the basis of the findings in Giemsa- and/or Papanicolaou- smears. More demanding diagnostic cases require preparation of additional cytological preparations. Therefore, it is convenient to suspend cytological samples in a liquid based medium (LBM) that preserve antigen and morphological properties. However, the duration of these properties being preserved in the medium is usually unknown. Eventually, cell morphology becomes impaired and altered, as well as antigen properties may be lost or become diffused. In this study, the influence of cytological sample storage length in in-house liquid based medium on antigen properties and cell morphology is evaluated. The question is how long the cytological samples in this medium can be stored so that the results of immunocytochemical reactions are still reliable and can be safely used in routine cytopathological diagnostics. The stability of 6 ICC markers that are most frequently used in everyday routine work were tested; Cytokeratin AE1/AE3, Calretinin, Epithelial specific antigen Ep-CAM (MOC-31), CD 45, Oestrogen receptor (ER), and Melanoma triple cocktail were tested on methanol fixed cytospins prepared from fresh fine needle aspiration biopsies, effusion samples, and disintegrated lymph nodes suspended in in-house cell medium. Cytospins were prepared on the day of the sampling as well as on the second, fourth, fifth, and eight day after sample collection. Next, they were fixed in methanol and immunocytochemically stained. Finally, the percentage of positive stained cells, reaction intensity, counterstaining, and cell morphology were assessed using two assessment methods: the internal assessment and the UK NEQAS ICC scheme assessment. Results show that the antigen properties for Cytokeratin AE1/AE3, MOC-31, CD 45, ER, and Melanoma triple cocktail were preserved even after 8 days of storage in in-house LBM, while the antigen properties for Calretinin remained unchanged only for 4 days. The key parameters for assessing detection of antigen are the proportion of cells with a positive reaction and intensity of staining. Well preserved cell morphology is highly important for reliable interpretation of ICC reaction. Therefore, it would be valuable to perform a similar analysis for other ICC markers to determine the duration in which the antigen and morphological properties are preserved in LBM. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cytology%20samples" title="cytology samples">cytology samples</a>, <a href="https://publications.waset.org/abstracts/search?q=cytospins" title=" cytospins"> cytospins</a>, <a href="https://publications.waset.org/abstracts/search?q=immunocytochemistry" title=" immunocytochemistry"> immunocytochemistry</a>, <a href="https://publications.waset.org/abstracts/search?q=liquid-based%20cytology" title=" liquid-based cytology"> liquid-based cytology</a> </p> <a href="https://publications.waset.org/abstracts/145540/immunocytochemical-stability-of-antigens-in-cytological-samples-stored-in-in-house-liquid-based-medium" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/145540.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">141</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Cytology Is a Promising Tool for the Diagnosis of High-Grade Serous Ovarian Carcinoma from Ascites</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Miceska%20Simona">Miceska Simona</a>, <a href="https://publications.waset.org/abstracts/search?q=%C5%A0kof%20Erik"> 艩kof Erik</a>, <a href="https://publications.waset.org/abstracts/search?q=Frkovi%C4%87%20Grazio%20Snje%C5%BEana"> Frkovi膰 Grazio Snje啪ana</a>, <a href="https://publications.waset.org/abstracts/search?q=Jeri%C4%8Devi%C4%87%20Anja"> Jeri膷evi膰 Anja</a>, <a href="https://publications.waset.org/abstracts/search?q=Smrkolj%20%C5%A0pela"> Smrkolj 艩pela</a>, <a href="https://publications.waset.org/abstracts/search?q=Cvjeti%C4%87anin%20Branko"> Cvjeti膰anin Branko</a>, <a href="https://publications.waset.org/abstracts/search?q=Novakovi%C4%87%20Srdjan"> Novakovi膰 Srdjan</a>, <a href="https://publications.waset.org/abstracts/search?q=Gr%C4%8Dar%20Kuzmanov%20Biljana"> Gr膷ar Kuzmanov Biljana</a>, <a href="https://publications.waset.org/abstracts/search?q=Kloboves-Prevodnik%20Veronika"> Kloboves-Prevodnik Veronika</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Objectives: High-grade serous ovarian cancer (HGSOC) is characterized by the dissemination of the tumor cells (TC) in the peritoneal cavity forming malignant ascites at the time of diagnosis or recurrence. Still, cytology itself has been underutilized as a modality for the diagnosis of HGSOC from ascites, and histological examination from the tumor tissue is yet the only validated method used. The objective of this study was to evaluate the reliability of cytology in the diagnosis of HGSOC in relation to the histopathological examination. Methods: The study included 42 patients with histologically confirmed HGSOC, accompanied by malignant ascites. To confirm the malignancy of the TC in the ascites and to define their immunophenotype, immunohistochemical reaction (IHC) of the following antigens: Calretinin, MOC, WT1, PAX8, p53, p16 & Ki-67 was evaluated on ascites cytospins and tissue blocks. For complete cytological determination of HGSOC, BRCA 1/2 gene mutation was determined from ascites, tissue block, and blood. BRCA1/2 mutation from blood was performed to define the type of mutation, somatic vs germline. Results: Among 42 patients, the immunophenotype of HGSOC from ascites was confirmed in 36 cases (86%). For more profound analysis, the patients were divided in 3 groups regarding the number of TC present in the ascites: patients with less than 10% TC, 10% TC, and more than 10% TC. From all included patients, in the group with less than 10% TC, there were 10 cases, and only 5 of them(50%) showed HGSOC phenotype; 12 cases had equally 10% of TC, and 11 cases (92%) showed HGSOC phenotype; 20 cases had more than 10% TC and all of them (100%) confirmed the HGSOC immunophenotype from ascites. Only 33 patients were eligible for further BRCA1/2 analysis. Eleven BRCA1/2 mutations were detected from thetissue block: 6 germline and 5 somatic. In 2 cases with less than 10% TC, BRCA1/2 mutation was not detected; 4 cases had 10% TC, and 2 of them (50%) confirmed the mutation; 4 cases had more than 10% TC, and all showed 100% reliability with the tumor tissue. Conclusions: Cytology is a highly reliable method for determining the immunophenotype of HGSOC and BRCA1/2 mutation if more than 10% of tumor cells are present in the ascites. This may present an additional non-invasive clinical approach for fast and effective diagnose in the future, especially in inoperable conditions or relapses. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cytology" title="cytology">cytology</a>, <a href="https://publications.waset.org/abstracts/search?q=ascites" title=" ascites"> ascites</a>, <a href="https://publications.waset.org/abstracts/search?q=high-grade%20serous%20ovarian%20cancer" title=" high-grade serous ovarian cancer"> high-grade serous ovarian cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=immunophenotype" title=" immunophenotype"> immunophenotype</a>, <a href="https://publications.waset.org/abstracts/search?q=BRCA1%2F2" title=" BRCA1/2"> BRCA1/2</a> </p> <a href="https://publications.waset.org/abstracts/144274/cytology-is-a-promising-tool-for-the-diagnosis-of-high-grade-serous-ovarian-carcinoma-from-ascites" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/144274.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">188</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul 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Search results for: cytospins