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expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); 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href="/sg/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-labeling.html">Nucleic Acid Labeling <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/dna-rna-purification-analysis/dna-rna-purification-analysis-equipment-plasticware.html">DNA & RNA Purification & Analysis Equipment & Plasticware <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/dna-rna-purification-analysis/protocol-videos.html">Nucleic Acid Isolation Protocol Videos <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-isolation-brochures.html">Nucleic Acid Isolation Brochures, Manuals and Guides <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-isolation-application-notes.html">Nucleic Acid Isolation Application Notes <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/dna-rna-purification-analysis/ivd-r-transition-updates.html">Sample Prep and Molecular Biology IVD-R Transition Updates <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/dna-rna-purification-analysis/learning-center.html">Learn about DNA & RNA extraction and analysis <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/dna-rna-purification-analysis/viral-pathogen.html">Infectious Disease Research <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/dna-rna-purification-analysis/sample-preparation-solutions-cancer-research.html">Sample Preparation Solutions for Cancer Research <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/dna-rna-purification-analysis/molecular-biology-bulk-reagents-lab-supplies.html">Molecular Biology Bulk Reagents and Lab Supplies for Sample Preparation <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/dna-rna-purification-analysis/spin-column-purification.html">Spin Column Purification <span class="nav-list-arrow">&rsaquo;</span></a></li></ul><script> if ( $('#navListActive').children('ul').length > 0 ) { var activeNav = $j('#navListActive'); $j('#navList').prepend(activeNav); } </script></div></div></div></div></div><div class="span9 main" role="main"><div class="MainParsys parsys"><div class="text parbase section"><p>Thermo Fisher Scientific offers a wide range of plasmid DNA extraction products that are designed to isolate plasmid DNA at the purity and scale you need. Choose from our selection of high-performing, cost-effective plasmid purification technologies designed to help you overcome common plasmid prep issues, such as low recovery and the presence of impurities. Learn how to manually purify plasmid DNA using our kits and our plasmid isolation protocol videos.<br></p></div><div class="well-cmp section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h2 id="what-is"> What is plasmid purification? </h2></div><div class="text parbase section"><p>Plasmid purification, also known as plasmid isolation or plasmid DNA isolation, refers to the process of extracting and purifying plasmid DNA from bacterial cells, allowing scientists to obtain a highly concentrated and pure form of the plasmid for further analysis and experimentation. The key steps in plasmid purification typically include bacterial cell lysis, removal of cellular debris, binding of plasmid DNA, washing, elution of plasmid DNA, and optional additional steps depending on the downstream application.</p></div></div></div></div></div></div><div class="heading section"><h2 id="prep"><br>Preparation sizes for plasmid DNA isolation </h2></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--1076509053"><tbody><tr><th width="20%"><span> </span></th><th width="16%" style="text-align: center;"><a href="/sg/en/home/life-science/dna-rna-purification-analysis/plasmid-isolation/plasmid-dna-mini-prep.html">Miniprep</a></th><th width="16%" style="text-align: center;"><a href="/sg/en/home/life-science/dna-rna-purification-analysis/plasmid-isolation/plasmid-dna-midiprep.html">Midiprep</a></th><th width="16%" style="text-align: center;"><a href="/sg/en/home/life-science/dna-rna-purification-analysis/plasmid-isolation/plasmid-dna-maxiprep.html">Maxiprep</a></th><th width="16%" style="text-align: center;">Megaprep</th><th width="16%" style="text-align: center;">Gigaprep</th></tr><tr><td><b>Overnight bacterial culture volume</b></td><td style="text-align: center;">1–5 mL</td><td style="text-align: center;">25–100 mL</td><td style="text-align: center;">100–500 mL</td><td style="text-align: center;">500 mL–2.5 L</td><td style="text-align: center;">2.5–5 L</td></tr><tr><td><b>Approximate yield</b></td><td style="text-align: center;">Up to 40 µg</td><td style="text-align: center;">Up to 400 µg</td><td style="text-align: center;">up to 1.5 mg</td><td style="text-align: center;">Up to 5 mg</td><td style="text-align: center;">Up to 15 mg</td></tr></tbody></table></div></div><div class="heading section"><h2 id="purity"><br>Purity grade and endotoxin level for plasmid DNA isolation </h2></div><div class="text parbase section"><p>When it comes to plasmid isolation, scientists have a range of options depending on their specific needs. Molecular-grade plasmid isolation technologies offer speed and cost-effectiveness, making them excellent for robust applications like cloning, nucleic acid labeling, PCR, and sequencing, where high purity levels are not essential. On the other hand, transfection-grade plasmid purification technologies are designed for applications that require greater purity and higher yields, such as transfection of standard cell lines and <i>in vitro</i> transcription. Transfection-grade plasmid DNA can also be used for molecular-grade applications. For the most sensitive applications, such as transfection of sensitive cell lines or <i>in vivo</i> research, advanced transfection-grade plasmid isolation technology is recommended. This technology helps ensure the highest purity and can also be used for all molecular and transfection-grade applications.</p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-1250312473"><tbody><tr><th width="25%" style="text-align: center;"><span>Purity grade</span></th><th width="25%" style="text-align: center;">Molecular</th><th width="25%" style="text-align: center;">Transfection</th><th width="25%" style="text-align: center;">Advanced transfection</th></tr><tr><td width="134" valign="top" style="text-align: center;">Endotoxin level</td><td width="134" valign="top" style="text-align: center;">>10 EU/µg</td><td width="134" valign="top" style="text-align: center;">0.1–1 EU/µg</td><td width="134" valign="top" style="text-align: center;"><0.1 EU/µg</td></tr></tbody></table></div></div><div class="text parbase section"><p><b>Learn more:</b> <a class="cta-link" href="/sg/en/home/life-science/dna-rna-purification-analysis/plasmid-isolation/transfection-grade-dna.html">Plasmid DNA purity grades</a></p></div><div class="text parbase section"><p>Robust purification of plasmid DNA, in the amount and purity required for the downstream application of interest, is the key step of any protein expression workflow. Learn how to obtain transfection-grade plasmids and use them with Gibco ExpiCHO and Expi293 transient mammalian expression systems.</p></div><div class="text parbase section"><p><b>Explore: </b><a class="cta-link" href="/sg/en/home/life-science/protein-biology/protein-expression/mammalian-protein-expression/transient-mammalian-protein-expression/expicho-expression-system.html">ExpiCHO Expression System</a></p></div><div class="heading section"><h2><br>Solutions for automated plasmid purification </h2></div><div class="text parbase section"><p><a href="/sg/en/home/life-science/dna-rna-purification-analysis/plasmid-isolation/automated-plasmid-purification.html"><b>Automated plasmid purification</b></a> is an advanced technology that utilizes robotics to streamline the process. Thermo Fisher provides automated plasmid purification solutions that offer high-throughput and centrifuge-free purification of transfection-grade plasmid DNA. These solutions minimize human error and help ensure reproducibility by precisely controlling purification steps and reagent volumes. For mini scale applications, specialized magnetic-bead purification kits are available for efficient plasmid DNA isolation on KingFisher platforms with liquid handling capabilities. Likewise, for maxi scale automation, the KingFisher PlasmidPro maxi processer offers a hands-free experience with prefilled cartridges. The purified plasmid DNA obtained through this process can be used for a range of downstream applications, including transfection, vector preparation, enzymatic modifications, cloning, sequencing, and<i> in vitro</i> transcription/translation.</p></div><div class="text parbase section"><div class="table-component-wrapper"><table cellpadding="0" cellspacing="0" border="1" class="rte-table" id="table--1327703647"><tbody><tr><td scope="col" rowspan="2"> </td><th scope="col"><h4 style="text-align: center;">Applied Biosystems MagMAX Pro HT NoSpin Miniprep Plasmid Purification Kit (coming soon)</h4></th><th scope="col"><h4 style="text-align: center;"><a href="/sg/en/home/life-science/dna-rna-purification-analysis/plasmid-isolation/automated-plasmid-purification.html">Thermo Scientific KingFisher Plasmid Pro</a></h4></th></tr><tr><td style="text-align: center;"><img src="/content/dam/LifeTech/global/life-sciences/DNARNAPurification/Images/2024/A58312.jpg" width="250px" title="MagMAX Pro HT NoSpin Miniprep kit" alt="image of magmax pro ht no spin kit"></td><td style="text-align: center;"><a href="/sg/en/home/life-science/dna-rna-purification-analysis/plasmid-isolation/automated-plasmid-purification.html"><img src="/content/dam/LifeTech/global/life-sciences/DNARNAPurification/Images/2024/PG3022-PJT11444-COL026729.jpg" width="150px" title="PlasmidPro" alt="image of plasmid pro instrument"></a></td></tr><tr><td><b>Prep sizes available</b></td><td style="text-align: center;">Mini</td><td style="text-align: center;">Maxi</td></tr><tr><td><b>Purity grade/Endotoxin level</b></td><td style="text-align: center;">Transfection grade<br></td><td style="text-align: center;">Transfection grade<br></td></tr></tbody></table></div></div><div class="heading section"><h2 id="transfection-grade"><br>Kits for transfection-grade plasmid DNA purification </h2></div><div class="text parbase section"><p>Transfection-grade plasmid isolation kits are specifically designed for scientists who need to extract plasmid DNA (pDNA) with minimal levels of endotoxins from bacterial cultures. During the plasmid purification process, large amounts of endotoxins are released from lysed bacteria, which can negatively impact cell growth, cell viability, transfection efficiency, protein expression, and even mimic sepsis. To address these concerns, our kits designed for low-endotoxin plasmid DNA isolation carefully remove these endotoxins, helping ensure the integrity of the isolated plasmid DNA. These kits offer a fast and easy method of plasmid purification, helping provide high purity and yield for sensitive downstream applications. Whether you are working with small or large prep sizes, our transfection-grade plasmid isolation kits are tailored to help meet your specific needs, enabling you achieve reliable and reproducible results. </p></div><div class="text parbase section"><div class="table-component-wrapper"><table border="0" cellspacing="0" cellpadding="0" width="0" class="rte-table" id="table-20537821"><tbody><tr><th width="17%"> </th><th width="30%" style="text-align: center;">Invitrogen PureLink Fast Low-Endotoxin Purification Kits</th><th width="30%" style="text-align: center;">Invitrogen PureLink HiPure Plasmid Kits</th><th width="30%" style="text-align: center;">PureLink HiPure Plasmid Filter Kits</th></tr><tr><td> </td><td style="text-align: center;"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2023/12/endotoxin-purification-kit.jpg" title="Invitrogen PureLink Fast Low-Endotoxin Purification Kit" alt="product" width="120px"></td><td style="text-align: center;"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2023/12/purelink-hipure-kit.jpg" title="Invitrogen PureLink HiPure Plasmid Kit" alt="product" width="120px"></td><td style="text-align: center;" width="60%"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2023/12/PureLink-HiPure-Plasmid-Filter-Kits-300x245.jpg" title="PureLink HiPure Plasmid Filter Midiprep Kit" alt="PureLink HiPure Plasmid Filter Midiprep Kit" width="120px"></td></tr><tr><td><b>Prep sizes available</b></td><td width="168" valign="top" style="text-align: center;"><a href="/order/catalog/product/A36227">Midi</a>, <a href="/order/catalog/product/A36228">Maxi</a></td><td width="163" valign="top" style="text-align: center;"><a href="/order/catalog/product/K210002">Mini</a>, <a href="/order/catalog/product/K210004">Midi</a>, <a href="/order/catalog/product/K210007">Maxi</a>, <a href="/order/catalog/product/K210008XP">Mega</a>, <a href="/order/catalog/product/K210009XP">Giga</a></td><td style="text-align: center;" width="60%"><a href="/order/catalog/product/K210014">Midi</a>, <a href="/order/catalog/product/K210016">Maxi</a></td></tr><tr><td><b>Purity grade/Endotoxin level</b></td><td width="168" valign="top" style="text-align: center;">Transfection grade<br> (0.1–1 EU/µg)</td><td width="163" valign="top" style="text-align: center;">Transfection grade<br> (0.1–1 EU/µg)</td><td style="text-align: center;" width="60%">Transfection grade<br> (0.1–1 EU/µg)</td></tr></tbody></table></div></div><div class="heading section"><h2 id="molecular-grade"><br>Kits for advanced transfection-grade plasmid DNA purification </h2></div><div class="text parbase section"><p>For scientists who require an even higher level of endotoxin removal, we offer advanced transfection-grade plasmid isolation kits. These kits go beyond the standard transfection-grade kits and provide an enhanced level of endotoxin removal, helping ensure the utmost purity of the isolated plasmid DNA. With our advanced transfection-grade plasmid isolation kits, you can confidently perform downstream applications that demand the highest quality pDNA. These kits are excellent for researchers working on critical experiments where even trace amounts of endotoxins can have a significant impact on their results. Using our advanced-transfection grade plasmid isolation kits can help eliminate the risks associated with endotoxins and obtain pure plasmid DNA for your scientific endeavors.</p></div><div class="text parbase section"><div class="table-component-wrapper"><table border="0" cellspacing="0" cellpadding="0" width="0" class="rte-table" id="table--1780577268"><tbody><tr><th width="17%"> </th><th width="20%" style="text-align: center;">Thermo Scientific GeneJET Endo-Free Plasmid Maxiprep Kit</th><th width="20%" style="text-align: center;">Invitrogen PureLink Expi Endotoxin-Free Plasmid Purification Kits</th></tr><tr><td> </td><td style="text-align: center;"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2023/12/endo-free-plasmid-maxiprep-kit.jpg" title="GeneJET Endo-Free Plasmid Maxiprep kit" alt="product" width="120px"></td><td style="text-align: center;"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2023/12/pure-link-endotoxin-free-plasmid-300x245.jpg" title="Invitrogen PureLink Expi Endotoxin-Free Plasmid Purification Kits" alt="product" width="120px"></td></tr><tr><td><b>Prep sizes available</b></td><td width="198" valign="top" style="text-align: center;"><a href="/order/catalog/product/K0861">Maxi</a></td><td width="219" valign="top" style="text-align: center;"><a href="/order/catalog/product/A33073">Maxi</a>, <a href="/order/catalog/product/A31232">Mega</a>, <a href="/order/catalog/product/A31233">Giga</a></td></tr><tr><td><b>Purity grade/Endotoxin level</b></td><td width="198" valign="top" style="text-align: center;">Advanced transfection grade<br> (<0.1 EU/µg)</td><td width="219" valign="top" style="text-align: center;">Advanced transfection grade<br> (<0.1 EU/µg)</td></tr></tbody></table></div></div><div class="heading section"><h2></br>Kits for molecular-grade plasmid purification </h2></div><div class="text parbase section"><p>For fast, easy, and reliable plasmid purification, our Invitrogen PureLink and GeneJET molecular-grade plasmid DNA (pDNA) isolation kits produce pure, ready-to-use plasmids that are compatible with a range of standard molecular biology applications. Molecular-grade kits are suitable for transformation of bacterial and yeast cells; however, they may not be well suited for transfection of sensitive mammalian cells due to the presence of endotoxins. For transfection applications, please see the transfection-grade plasmid purification kits above.</p></div><div class="text parbase section"><div class="table-component-wrapper"><table border="0" cellspacing="0" cellpadding="0" width="0" class="rte-table" id="table--732949850"><tbody><tr><th width="17%"> </th><th width="20%" style="text-align: center;"><b>Invitrogen PureLink Pro Quick 96 Plasmid Purification Kit</b></th><th width="20%" style="text-align: center;"><b>Invitrogen PureLink Quick Plasmid Miniprep Kit</b></th><th width="20%" style="text-align: center;"><b>Thermo Scientific GeneJET Plasmid Kits</b></th></tr><tr><td> </td><td style="text-align: center;"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2023/12/quick-plasmid-purification-kit.jpg" title="Invitrogen PureLink Pro Quick 96 Plasmid Purification Kit" alt="product" width="120px"></td><td style="text-align: center;"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2023/12/quick-plasmid-miniprep-kit.jpg" title="Invitrogen PureLink Quick Plasmid Miniprep Kit" alt="product" width="120px"></td><td style="text-align: center;"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2023/12/genejet-mini-plasmid-prep.jpg" title="GeneJET Plasmid Kit" alt="product" width="120px"></td></tr><tr><td><b>Prep size available</b></td><td width="198" valign="top" style="text-align: center;"><a href="/order/catalog/product/K211004A">Small-scale 96-well plate format</a></td><td width="219" valign="top" style="text-align: center;"><a href="/order/catalog/product/K210011">Mini</a></td><td width="192" valign="top" style="text-align: center;"><a href="/order/catalog/product/K0503">Mini</a>, <a href="/order/catalog/product/K0481">Midi</a>, <a href="/order/catalog/product/K0492">Maxi</a></td></tr><tr><td><b>Purity grade/Endotoxin level</b></td><td width="198" valign="top" style="text-align: center;">Molecular grade<br> (>10 EU/µg)</td><td width="219" valign="top" style="text-align: center;">Molecular grade<br> (>10 EU/µg)</td><td width="192" valign="top" style="text-align: center;">Molecular grade<br> (>10 EU/µg)</td></tr></tbody></table></div></div><div class="heading section"><h2 id="additional-buffers"><br>Additional buffers for plasmid isolation </h2></div><div class="text parbase section"><ul><li><a href="/order/catalog/product/K210018">PureLink HiPure BAC Buffer Kit</a></li><li><a href="/order/catalog/product/A33074">PureLink Expi Endotoxin-Free Buffer Set</a><br></li></ul></div><div class="heading section"><h2 id="videos"><br>Plasmid purification—How-to videos </h2></div><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3 id="videos"> Extract plasmid DNA with PureLink and GeneJet kits </h3></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="brightcoveplayer section"><div data-video="9db4408cb862"><script type="text/javascript"> var loadEME = true; 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margin-left: auto; margin-right: auto; margin-top: 0; overflow-x: hidden; overflow-y: hidden; text-align: center; width: 100%; text-align: center;"><div id="container-9db4408cb862" class="brightcove-container"><script>document.createElement('video')</script><video id="video-9db4408cb862" data-account="3663210762001" data-player="08UsfMRkC" data-embed="default" data-video-id="5668962082001" class="video-js" controls ></video><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script> videojs.options.techOrder = ["html5"]; </script></div></div></div></div><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "How to do an Endo-free Maxi Prep", "description": "Watch a 4-minute video on how to purify Plasmid DNA. 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Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div><div container-id="cq-colctrl-lt0" comp-id="A2"><div class="heading section"><h3> How to purify endotoxin-free plasmid DNA </h3></div></div><div container-id="cq-colctrl-lt0" comp-id="A3"><div class="text parbase section"><p>Watch this video to learn how to purify plasmid DNA using the <a href="/order/catalog/product/A31231" target="_blank">PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit</a>. This video shows you how to isolate and purify high-quality, endotoxin-free plasmid DNA for sensitive applications like primary cell transfection.</p></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="brightcoveplayer section"><div data-video="74d8901034b9"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); // Call the provided callback function when the value is true }) .catch((error) => { console.error('Error:', error); }); } checkVideojsLoaded(function(){ videojs.options.techOrder = ["html5"]; }, 'video-74d8901034b9', 10); </script></div><div class="video-container video-single"><div id="span-video-74d8901034b9" class="video-player-wrapper"><div style="margin-bottom: 0; margin-left: auto; margin-right: auto; margin-top: 0; overflow-x: hidden; overflow-y: hidden; text-align: center; width: 100%; text-align: center;"><div id="container-74d8901034b9" class="brightcove-container"><script>document.createElement('video')</script><video id="video-74d8901034b9" data-account="3663210762001" data-player="08UsfMRkC" data-embed="default" data-video-id="5152740817001" class="video-js" controls ></video><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script> videojs.options.techOrder = ["html5"]; </script></div></div></div></div><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "How to Purify Molecular Grade Plasmid DNA", "description": "Learn how to purify molecular grade plasmid DNA in 15 minutes with the Thermo Scientific GeneJET Plasmid Miniprep Kit. 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Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div><div container-id="cq-colctrl-lt0" comp-id="B2"><div class="heading section"><h3> How to purify molecular-grade plasmid DNA </h3></div></div><div container-id="cq-colctrl-lt0" comp-id="B3"><div class="text parbase section"><p>This video shows you how to purify molecular-grade plasmid DNA in 15 minutes using our <a href="/order/catalog/product/K0503" target="_blank">GeneJET Plasmid Miniprep Kit</a>. Molecular-grade plasmid DNA is suitable for cloning, nucleic acid labeling, PCR, DNA sequencing, and transformation.</p></div></div></div></div><div style="clear:both"></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="brightcoveplayer section"><div data-video="c7cfd0bdf944"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); // Call the provided callback function when the value is true }) .catch((error) => { console.error('Error:', error); }); } checkVideojsLoaded(function(){ videojs.options.techOrder = ["html5"]; }, 'video-c7cfd0bdf944', 10); </script></div><div class="video-container video-single"><div id="span-video-c7cfd0bdf944" class="video-player-wrapper"><div style="margin-bottom: 0; margin-left: auto; margin-right: auto; margin-top: 0; overflow-x: hidden; overflow-y: hidden; text-align: center; width: 100%; text-align: center;"><div id="container-c7cfd0bdf944" class="brightcove-container"><script>document.createElement('video')</script><video id="video-c7cfd0bdf944" data-account="3663210762001" data-player="08UsfMRkC" data-embed="default" data-video-id="5152740821001" class="video-js" controls ></video><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script> videojs.options.techOrder = ["html5"]; </script></div></div></div></div><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "How to do a megaprep plasmid DNA purification", "description": "Isolate transfection-grade plasmid DNA in as little as 90 minutes with the PureLink HiPure Expi Plasmid Megaprep Kit. 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This innovative proprietary anion-exchange resin in these kits combines large capacity with fast flow rate, high resolution, and efficiency.</p></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="brightcoveplayer section"><div data-video="014064c267ec"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); // Call the provided callback function when the value is true }) .catch((error) => { console.error('Error:', error); }); } checkVideojsLoaded(function(){ videojs.options.techOrder = ["html5"]; }, 'video-014064c267ec', 10); </script></div><div class="video-container video-single"><div id="span-video-014064c267ec" class="video-player-wrapper"><div style="margin-bottom: 0; margin-left: auto; margin-right: auto; margin-top: 0; overflow-x: hidden; overflow-y: hidden; text-align: center; width: 100%; text-align: center;"><div id="container-014064c267ec" class="brightcove-container"><script>document.createElement('video')</script><video id="video-014064c267ec" data-account="3663210762001" data-player="08UsfMRkC" data-embed="default" data-video-id="5152740816001" class="video-js" controls ></video><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script> videojs.options.techOrder = ["html5"]; </script></div></div></div></div><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "How to purify transfection-grade plasmid DNA", "description": "Learn how to purify transfection grade plasmid DNA with the Invitrogen PureLink HiPure Plasmid Filter Maxiprep Kit. 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Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div><div container-id="cq-colctrl-lt0" comp-id="B2"><div class="heading section"><h3> How to purify transfection grade plasmid DNA </h3></div></div><div container-id="cq-colctrl-lt0" comp-id="B3"><div class="text parbase section"><p>Learn how to purify transfection grade plasmid DNA with the Invitrogen <a href="/order/catalog/product/K210016" target="_blank">PureLink HiPure Plasmid Filter Maxiprep Kit</a>. Transfection grade plasmid DNA is suitable for most molecular-grade applications, as well as <i>in vitro </i>transcription and transfection.</p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div><div><div class="MainInheritParsys iparsys parsys"><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="well-cmp well-default well-gray-lighter"><div class="well well-gray-lighter well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><div class="h2" id="resources"> Resources </div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><p><b><a href="/sg/en/home/technical-resources/technical-reference-library/nucleic-acid-purification-analysis-support-center.html">Nucleic Acid Purification and Analysis Support Center</a></b><br> Find tips, troubleshooting help, and resources for your nucleic acid purification & analysis applications.</p><ul class="the-icons"><li><a href="/sg/en/home/products-and-services/promotions/life-science/everyday-essentials/transfection.html">Transfection Workflow Essentials</a></li><li><a href="/sg/en/home/life-science/dna-rna-purification-analysis/learning-center.html">DNA & RNA Learning Center</a></li><li><span class="icon-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/rna-isolation-purification-brochure.pdf" target="_blank">RNA Purification and Analysis Guide</a></li><li><a href="/sg/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-isolation-brochures.html" class="cta-link">See all nucleic acid isolation brochures, manuals and guides</a></li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><ul class="unstyled"><li><a href="/sg/en/home/life-science/dna-rna-purification-analysis/protocol-videos.html">Protocol Videos</a>—Videos to help you isolate nucleic acids using a variety of techniques.</li><li><a href="/sg/en/home/life-science/dna-rna-purification-analysis.html">DNA & RNA Selection Guide</a>—Find the right purification products.</li><li><a href="https://videos.thermofisher.com/category/videos/dna-rna-extraction-analysis-videos" target="_blank">Videos</a>—View our library of DNA & RNA purification and analysis videos.</li><li><a href="/sg/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-isolation-application-notes.html">Application Notes</a>—Explore our application notes from scientists sharing data for isolation products</li></ul></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="ltrawhtml_d1d9 LTRawHTML"><style> /* Remove horizontal scroll on hero banner table */ .wellPageHeading .well-container .table-component-wrapper table {width:100% !important;} .content.wide-template .container-wrap.template-2-column .span3.sidebar > .container-leftnav {margin-right:20px;} /*.railsection {padding-right:10px;}*/ .textimage {margin-bottom:0px;} /* Label display for author */ .ux-tool-label { display: none; border-left: 50px #dcdcdc solid; padding-left: 10px; font-style: italic; font-size: 14px; } .cq-wcm-edit .ux-tool-label {display: block;} .wellPageHeading h1 b, .wellPageHeading h1 strong {font-weight:normal !important;} .wellPageHeading table td > * {text-shadow: none !important;} </style><div class="ux-tool-label ">Stylesheet for Classic Wide Template adjustments</div></div></div></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="ltrawhtml_a06 LTRawHTML"><style> /* Hero Banner Dark Grandient */ .wellPageHeading .well-content { background-image: linear-gradient(-45deg,rgba(68,68,68,0),rgba(0,0,0,.686)); } /* Label display for author */ .ux-tool-label { display: none; border-left: 50px #dcdcdc solid; padding-left: 10px; font-style: italic; font-size: 14px; } .cq-wcm-edit .ux-tool-label {display: block;} </style><div class="ux-tool-label ">Hero banner dark gradient overlay</div></div></div></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="text_72be text parbase"><p>For Research Use Only. 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