Zebrafish as a Model for Investigating Antiviral Innate Immunity

<!DOCTYPE html> <html lang="en" class="no-js"> <head> <meta charset="UTF-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <meta name="viewport" content="width=device-width, initial-scale=1"> <meta name="applicable-device" content="pc,mobile"> <meta name="access" content="No"> <meta name="twitter:site" content="SpringerLink"/> <meta name="twitter:card" content="summary"/> <meta name="twitter:image:alt" content="Content cover image"/> <meta name="twitter:title" content="Zebrafish as a Model for Investigating Antiviral Innate Immunity"/> <meta name="twitter:description" content="Zebrafish is a widely used model organism in genetics, developmental biology, pathology, and immunology research. Due to their fast reproduction, large numbers, transparent early embryos, and high genetic conservation with the human genome, zebrafish have been used..."/> <meta name="twitter:image" content="https://static-content.springer.com/cover/book/978-1-0716-4108-8.jpg"/> <meta name="dc.identifier" content="10.1007/978-1-0716-4108-8_22"/> <meta name="DOI" content="10.1007/978-1-0716-4108-8_22"/> <meta name="dc.description" content="Zebrafish is a widely used model organism in genetics, developmental biology, pathology, and immunology research. Due to their fast reproduction, large numbers, transparent early embryos, and high genetic conservation with the human genome, zebrafish have been used..."/> <meta name="citation_pdf_url" content="https://link.springer.com/content/pdf/10.1007/978-1-0716-4108-8_22.pdf"/> <meta name="citation_fulltext_html_url" content="https://link.springer.com/protocol/10.1007/978-1-0716-4108-8_22"/> <meta name="citation_abstract_html_url" content="https://link.springer.com/protocol/10.1007/978-1-0716-4108-8_22"/> <meta name="citation_inbook_title" content="Antiviral Innate Immunity"/> <meta name="citation_title" content="Zebrafish as a Model for Investigating Antiviral Innate Immunity"/> <meta name="citation_publication_date" content="2025"/> <meta name="citation_firstpage" content="221"/> <meta name="citation_lastpage" content="236"/> <meta name="citation_language" content="en"/> <meta name="citation_doi" content="10.1007/978-1-0716-4108-8_22"/> <meta name="citation_issn" content="1940-6029"/> <meta name="citation_isbn" content="978-1-0716-4108-8"/> <meta name="size" content="156950"/> <meta name="description" content="Zebrafish is a widely used model organism in genetics, developmental biology, pathology, and immunology research. Due to their fast reproduction, large numbers, transparent early embryos, and high genetic conservation with the human genome, zebrafish have been used..."/> <meta name="citation_author" content="Wu, Xiao Man"/> <meta name="citation_author_institution" content="Institute of Hydrobiology, Chinese Academy of Sciences"/> <meta name="citation_author" content="Zheng, Si Yao"/> <meta name="citation_author_institution" content="Institute of Hydrobiology, Chinese Academy of Sciences"/> <meta name="citation_author_institution" content="University of Chinese Academy of Sciences"/> <meta name="citation_author" content="Chang, Ming Xian"/> <meta name="citation_author_email" content="mingxianchang@ihb.ac.cn"/> <meta name="citation_author_institution" content="Institute of Hydrobiology, Chinese Academy of Sciences"/> <meta name="citation_author_institution" content="University of Chinese Academy of Sciences"/> <meta name="citation_publisher" content="Humana, New York, NY"/> <meta name="citation_springer_api_url" content="http://api.springer.com/xmldata/jats?q=doi:10.1007/978-1-0716-4108-8_22&api_key="/> <meta name="format-detection" content="telephone=no"/> <meta property="og:url" content="https://link.springer.com/protocol/10.1007/978-1-0716-4108-8_22"/> <meta property="og:type" content="Paper"/> <meta property="og:site_name" content="SpringerLink"/> <meta property="og:title" content="Zebrafish as a Model for Investigating Antiviral Innate Immunity"/> <meta property="og:description" content="Zebrafish is a widely used model organism in genetics, developmental biology, pathology, and immunology research. Due to their fast reproduction, large numbers, transparent early embryos, and high genetic conservation with the human genome, zebrafish have been used..."/> <meta property="og:image" content="https://static-content.springer.com/cover/book/978-1-0716-4108-8.jpg"/> <title>Zebrafish as a Model for Investigating Antiviral Innate Immunity | SpringerLink</title> <link rel="apple-touch-icon" sizes="180x180" href=/oscar-static/img/favicons/darwin/apple-touch-icon-92e819bf8a.png> <link rel="icon" type="image/png" sizes="192x192" href=/oscar-static/img/favicons/darwin/android-chrome-192x192-6f081ca7e5.png> <link rel="icon" type="image/png" sizes="32x32" href=/oscar-static/img/favicons/darwin/favicon-32x32-1435da3e82.png> <link rel="icon" type="image/png" sizes="16x16" href=/oscar-static/img/favicons/darwin/favicon-16x16-ed57f42bd2.png> <link rel="shortcut icon" data-test="shortcut-icon" href=/oscar-static/img/favicons/darwin/favicon-c6d59aafac.ico> <meta name="theme-color" content="#e6e6e6"> <script>(function(H){H.className=H.className.replace(/\bno-js\b/,'js')})(document.documentElement)</script>   <link rel="stylesheet" media="print" href=/oscar-static/app-springerlink/css/print-b8af42253b.css> <style> html{text-size-adjust:100%;line-height:1.15}body{font-family:Merriweather Sans,Helvetica Neue,Helvetica,Arial,sans-serif;line-height:1.8;margin:0}details,main{display:block}h1{font-size:2em;margin:.67em 0}a{background-color:transparent;color:#025e8d}sub{bottom:-.25em;font-size:75%;line-height:0;position:relative;vertical-align:baseline}img{border:0;height:auto;max-width:100%;vertical-align:middle}button,input{font-family:inherit;font-size:100%;line-height:1.15;margin:0;overflow:visible}button{text-transform:none}[type=button],[type=submit],button{-webkit-appearance:button}[type=search]{-webkit-appearance:textfield;outline-offset:-2px}summary{display:list-item}[hidden]{display:none}button{cursor:pointer}svg{height:1rem;width:1rem} </style> <style>@media only print, only all and (prefers-color-scheme: no-preference), only all and (prefers-color-scheme: light), only all and (prefers-color-scheme: dark) { body{background:#fff;color:#222;font-family:Merriweather Sans,Helvetica Neue,Helvetica,Arial,sans-serif;line-height:1.8;min-height:100%}a{color:#025e8d;text-decoration:underline;text-decoration-skip-ink:auto}button{cursor:pointer}img{border:0;height:auto;max-width:100%;vertical-align:middle}html{box-sizing:border-box;font-size:100%;height:100%;overflow-y:scroll}h1{font-size:2.25rem}h2{font-size:1.75rem}h1,h2,h4{font-weight:700;line-height:1.2}h4{font-size:1.25rem}body{font-size:1.125rem}*{box-sizing:inherit}p{margin-bottom:2rem;margin-top:0}p:last-of-type{margin-bottom:0}.c-ad{text-align:center}@media only screen and (min-width:480px){.c-ad{padding:8px}}.c-ad--728x90{display:none}.c-ad--728x90 .c-ad__inner{min-height:calc(1.5em + 94px)}@media only screen and (min-width:876px){.js .c-ad--728x90{display:none}}.c-ad__label{color:#333;font-size:.875rem;font-weight:400;line-height:1.5;margin-bottom:4px}.c-ad__label,.c-status-message{font-family:Merriweather Sans,Helvetica Neue,Helvetica,Arial,sans-serif}.c-status-message{align-items:center;box-sizing:border-box;display:flex;position:relative;width:100%}.c-status-message :last-child{margin-bottom:0}.c-status-message--boxed{background-color:#fff;border:1px solid #ccc;line-height:1.4;padding:16px}.c-status-message__heading{font-family:Merriweather Sans,Helvetica Neue,Helvetica,Arial,sans-serif;font-size:.875rem;font-weight:700}.c-status-message__icon{fill:currentcolor;display:inline-block;flex:0 0 auto;height:1.5em;margin-right:8px;transform:translate(0);vertical-align:text-top;width:1.5em}.c-status-message__icon--top{align-self:flex-start}.c-status-message--info .c-status-message__icon{color:#003f8d}.c-status-message--boxed.c-status-message--info{border-bottom:4px solid #003f8d}.c-status-message--error .c-status-message__icon{color:#c40606}.c-status-message--boxed.c-status-message--error{border-bottom:4px solid #c40606}.c-status-message--success .c-status-message__icon{color:#00b8b0}.c-status-message--boxed.c-status-message--success{border-bottom:4px solid #00b8b0}.c-status-message--warning .c-status-message__icon{color:#edbc53}.c-status-message--boxed.c-status-message--warning{border-bottom:4px solid #edbc53}.eds-c-header{background-color:#fff;border-bottom:2px solid #01324b;font-family:Merriweather Sans,Helvetica Neue,Helvetica,Arial,sans-serif;font-size:1rem;line-height:1.5;padding:8px 0 0}.eds-c-header__container{align-items:center;display:flex;flex-wrap:nowrap;gap:8px 16px;justify-content:space-between;margin:0 auto 8px;max-width:1280px;padding:0 8px;position:relative}.eds-c-header__nav{border-top:2px solid #c5e0f4;padding-top:4px;position:relative}.eds-c-header__nav-container{align-items:center;display:flex;flex-wrap:wrap;margin:0 auto 4px;max-width:1280px;padding:0 8px;position:relative}.eds-c-header__nav-container>:not(:last-child){margin-right:32px}.eds-c-header__link-container{align-items:center;display:flex;flex:1 0 auto;gap:8px 16px;justify-content:space-between}.eds-c-header__list{list-style:none;margin:0;padding:0}.eds-c-header__list-item{font-weight:700;margin:0 auto;max-width:1280px;padding:8px}.eds-c-header__list-item:not(:last-child){border-bottom:2px solid #c5e0f4}.eds-c-header__item{color:inherit}@media only screen and (min-width:768px){.eds-c-header__item--menu{display:none;visibility:hidden}.eds-c-header__item--menu:first-child+*{margin-block-start:0}}.eds-c-header__item--inline-links{display:none;visibility:hidden}@media only screen and (min-width:768px){.eds-c-header__item--inline-links{display:flex;gap:16px 16px;visibility:visible}}.eds-c-header__item--divider:before{border-left:2px solid #c5e0f4;content:"";height:calc(100% - 16px);margin-left:-15px;position:absolute;top:8px}.eds-c-header__brand{padding:16px 8px}.eds-c-header__brand a{display:block;line-height:1;text-decoration:none}.eds-c-header__brand img{height:1.5rem;width:auto}.eds-c-header__link{color:inherit;display:inline-block;font-weight:700;padding:16px 8px;position:relative;text-decoration-color:transparent;white-space:nowrap;word-break:normal}.eds-c-header__icon{fill:currentcolor;display:inline-block;font-size:1.5rem;height:1em;transform:translate(0);vertical-align:bottom;width:1em}.eds-c-header__icon+*{margin-left:8px}.eds-c-header__expander{background-color:#f0f7fc}.eds-c-header__search{display:block;padding:24px 0}@media only screen and (min-width:768px){.eds-c-header__search{max-width:70%}}.eds-c-header__search-container{position:relative}.eds-c-header__search-label{color:inherit;display:inline-block;font-weight:700;margin-bottom:8px}.eds-c-header__search-input{background-color:#fff;border:1px solid #000;padding:8px 48px 8px 8px;width:100%}.eds-c-header__search-button{background-color:transparent;border:0;color:inherit;height:100%;padding:0 8px;position:absolute;right:0}.has-tethered.eds-c-header__expander{border-bottom:2px solid #01324b;left:0;margin-top:-2px;top:100%;width:100%;z-index:10}@media only screen and (min-width:768px){.has-tethered.eds-c-header__expander--menu{display:none;visibility:hidden}}.has-tethered .eds-c-header__heading{display:none;visibility:hidden}.has-tethered .eds-c-header__heading:first-child+*{margin-block-start:0}.has-tethered .eds-c-header__search{margin:auto}.eds-c-header__heading{margin:0 auto;max-width:1280px;padding:16px 16px 0}.eds-c-pagination{align-items:center;display:flex;flex-wrap:wrap;font-family:Merriweather Sans,Helvetica Neue,Helvetica,Arial,sans-serif;font-size:.875rem;gap:16px 0;justify-content:center;line-height:1.4;list-style:none;margin:0;padding:32px 0}@media only screen and (min-width:480px){.eds-c-pagination{padding:32px 16px}}.eds-c-pagination__item{margin-right:8px}.eds-c-pagination__item--prev{margin-right:16px}.eds-c-pagination__item--next .eds-c-pagination__link,.eds-c-pagination__item--prev .eds-c-pagination__link{padding:16px 8px}.eds-c-pagination__item--next{margin-left:8px}.eds-c-pagination__item:last-child{margin-right:0}.eds-c-pagination__link{align-items:center;color:#222;cursor:pointer;display:inline-block;font-size:1rem;margin:0;padding:16px 24px;position:relative;text-align:center;transition:all .2s ease 0s}.eds-c-pagination__link:visited{color:#222}.eds-c-pagination__link--disabled{border-color:#555;color:#555;cursor:default}.eds-c-pagination__link--active{background-color:#01324b;background-image:none;border-radius:8px;color:#fff}.eds-c-pagination__link--active:focus,.eds-c-pagination__link--active:hover,.eds-c-pagination__link--active:visited{color:#fff}.eds-c-pagination__link-container{align-items:center;display:flex}.eds-c-pagination__icon{fill:#222;height:1.5rem;width:1.5rem}.eds-c-pagination__icon--disabled{fill:#555}.eds-c-pagination__visually-hidden{clip:rect(0,0,0,0);border:0;clip-path:inset(50%);height:1px;overflow:hidden;padding:0;position:absolute!important;white-space:nowrap;width:1px}.c-breadcrumbs{color:#333;font-family:Merriweather Sans,Helvetica Neue,Helvetica,Arial,sans-serif;font-size:1rem;list-style:none;margin:0;padding:0}.c-breadcrumbs>li{display:inline}svg.c-breadcrumbs__chevron{fill:#333;height:10px;margin:0 .25rem;width:10px}.c-breadcrumbs--contrast,.c-breadcrumbs--contrast .c-breadcrumbs__link{color:#fff}.c-breadcrumbs--contrast svg.c-breadcrumbs__chevron{fill:#fff}@media only screen and (max-width:479px){.c-breadcrumbs .c-breadcrumbs__item{display:none}.c-breadcrumbs .c-breadcrumbs__item:last-child,.c-breadcrumbs .c-breadcrumbs__item:nth-last-child(2){display:inline}}.c-skip-link{background:#01324b;bottom:auto;color:#fff;font-family:Merriweather Sans,Helvetica Neue,Helvetica,Arial,sans-serif;font-size:1rem;padding:8px;position:absolute;text-align:center;transform:translateY(-100%);width:100%;z-index:9999}@media (prefers-reduced-motion:reduce){.c-skip-link{transition:top .3s ease-in-out 0s}}@media print{.c-skip-link{display:none}}.c-skip-link:active,.c-skip-link:hover,.c-skip-link:link,.c-skip-link:visited{color:#fff}.c-skip-link:focus{transform:translateY(0)}.l-with-sidebar{display:flex;flex-wrap:wrap}.l-with-sidebar>*{margin:0}.l-with-sidebar__sidebar{flex-basis:var(--with-sidebar--basis,400px);flex-grow:1}.l-with-sidebar>:not(.l-with-sidebar__sidebar){flex-basis:0px;flex-grow:999;min-width:var(--with-sidebar--min,53%)}.l-with-sidebar>:first-child{padding-right:4rem}@supports (gap:1em){.l-with-sidebar>:first-child{padding-right:0}.l-with-sidebar{gap:var(--with-sidebar--gap,4rem)}}.c-header__link{color:inherit;display:inline-block;font-weight:700;padding:16px 8px;position:relative;text-decoration-color:transparent;white-space:nowrap;word-break:normal}.app-masthead__colour-4{--background-color:#ff9500;--gradient-light:rgba(0,0,0,.5);--gradient-dark:rgba(0,0,0,.8)}.app-masthead{background:var(--background-color,#0070a8);position:relative}.app-masthead:after{background:radial-gradient(circle at top right,var(--gradient-light,rgba(0,0,0,.4)),var(--gradient-dark,rgba(0,0,0,.7)));bottom:0;content:"";left:0;position:absolute;right:0;top:0}@media only screen and (max-width:479px){.app-masthead:after{background:linear-gradient(225deg,var(--gradient-light,rgba(0,0,0,.4)),var(--gradient-dark,rgba(0,0,0,.7)))}}.app-masthead__container{color:var(--masthead-color,#fff);margin:0 auto;max-width:1280px;padding:0 16px;position:relative;z-index:1}.u-button{align-items:center;background-color:#01324b;background-image:none;border:4px solid transparent;border-radius:32px;cursor:pointer;display:inline-flex;font-family:Merriweather Sans,Helvetica Neue,Helvetica,Arial,sans-serif;font-size:.875rem;font-weight:700;justify-content:center;line-height:1.3;margin:0;padding:16px 32px;position:relative;transition:all .2s ease 0s;width:auto}.u-button svg,.u-button--contrast svg,.u-button--primary svg,.u-button--secondary svg,.u-button--tertiary svg{fill:currentcolor}.u-button,.u-button:visited{color:#fff}.u-button,.u-button:hover{box-shadow:0 0 0 1px #01324b;text-decoration:none}.u-button:hover{border:4px solid #fff}.u-button:focus{border:4px solid #fc0;box-shadow:none;outline:0;text-decoration:none}.u-button:focus,.u-button:hover{background-color:#fff;background-image:none;color:#01324b}.app-masthead--pastel .c-pdf-download .u-button--primary:focus svg path,.app-masthead--pastel .c-pdf-download .u-button--primary:hover svg path,.c-context-bar--sticky .c-context-bar__container .c-pdf-download .u-button--primary:focus svg path,.c-context-bar--sticky .c-context-bar__container .c-pdf-download .u-button--primary:hover svg path,.u-button--primary:focus svg path,.u-button--primary:hover svg path,.u-button:focus svg path,.u-button:hover svg path{fill:#01324b}.u-button--primary{background-color:#01324b;background-image:none;border:4px solid transparent;box-shadow:0 0 0 1px #01324b;color:#fff;font-weight:700}.u-button--primary:visited{color:#fff}.u-button--primary:hover{border:4px solid #fff;box-shadow:0 0 0 1px #01324b;text-decoration:none}.u-button--primary:focus{border:4px solid #fc0;box-shadow:none;outline:0;text-decoration:none}.u-button--primary:focus,.u-button--primary:hover{background-color:#fff;background-image:none;color:#01324b}.u-button--secondary{background-color:#fff;border:4px solid #fff;color:#01324b;font-weight:700}.u-button--secondary:visited{color:#01324b}.u-button--secondary:hover{border:4px solid #01324b;box-shadow:none}.u-button--secondary:focus,.u-button--secondary:hover{background-color:#01324b;color:#fff}.app-masthead--pastel .c-pdf-download .u-button--secondary:focus svg path,.app-masthead--pastel .c-pdf-download .u-button--secondary:hover svg path,.c-context-bar--sticky .c-context-bar__container .c-pdf-download .u-button--secondary:focus svg path,.c-context-bar--sticky .c-context-bar__container .c-pdf-download .u-button--secondary:hover svg path,.u-button--secondary:focus svg path,.u-button--secondary:hover svg path,.u-button--tertiary:focus svg path,.u-button--tertiary:hover svg path{fill:#fff}.u-button--tertiary{background-color:#ebf1f5;border:4px solid transparent;box-shadow:none;color:#666;font-weight:700}.u-button--tertiary:visited{color:#666}.u-button--tertiary:hover{border:4px solid #01324b;box-shadow:none}.u-button--tertiary:focus,.u-button--tertiary:hover{background-color:#01324b;color:#fff}.u-button--contrast{background-color:transparent;background-image:none;color:#fff;font-weight:400}.u-button--contrast:visited{color:#fff}.u-button--contrast,.u-button--contrast:focus,.u-button--contrast:hover{border:4px solid #fff}.u-button--contrast:focus,.u-button--contrast:hover{background-color:#fff;background-image:none;color:#000}.u-button--contrast:focus svg path,.u-button--contrast:hover svg path{fill:#000}.u-button--disabled,.u-button:disabled{background-color:transparent;background-image:none;border:4px solid #ccc;color:#000;cursor:default;font-weight:400;opacity:.7}.u-button--disabled svg,.u-button:disabled svg{fill:currentcolor}.u-button--disabled:visited,.u-button:disabled:visited{color:#000}.u-button--disabled:focus,.u-button--disabled:hover,.u-button:disabled:focus,.u-button:disabled:hover{border:4px solid #ccc;text-decoration:none}.u-button--disabled:focus,.u-button--disabled:hover,.u-button:disabled:focus,.u-button:disabled:hover{background-color:transparent;background-image:none;color:#000}.u-button--disabled:focus svg path,.u-button--disabled:hover svg path,.u-button:disabled:focus svg path,.u-button:disabled:hover svg path{fill:#000}.u-button--small,.u-button--xsmall{font-size:.875rem;padding:2px 8px}.u-button--small{padding:8px 16px}.u-button--large{font-size:1.125rem;padding:10px 35px}.u-button--full-width{display:flex;width:100%}.u-button--icon-left svg{margin-right:8px}.u-button--icon-right svg{margin-left:8px}.u-clear-both{clear:both}.u-container{margin:0 auto;max-width:1280px;padding:0 16px}.u-justify-content-space-between{justify-content:space-between}.u-display-none{display:none}.js .u-js-hide,.u-hide{display:none;visibility:hidden}.u-visually-hidden{clip:rect(0,0,0,0);border:0;clip-path:inset(50%);height:1px;overflow:hidden;padding:0;position:absolute!important;white-space:nowrap;width:1px}.u-icon{fill:currentcolor;display:inline-block;height:1em;transform:translate(0);vertical-align:text-top;width:1em}.u-list-reset{list-style:none;margin:0;padding:0}.u-ma-16{margin:16px}.u-mt-0{margin-top:0}.u-mt-24{margin-top:24px}.u-mt-32{margin-top:32px}.u-mb-8{margin-bottom:8px}.u-mb-32{margin-bottom:32px}.u-button-reset{background-color:transparent;border:0;padding:0}.u-sans-serif{font-family:Merriweather Sans,Helvetica Neue,Helvetica,Arial,sans-serif}.u-serif{font-family:Merriweather,serif}h1,h2,h4{-webkit-font-smoothing:antialiased}p{overflow-wrap:break-word;word-break:break-word}.u-h4{font-size:1.25rem;font-weight:700;line-height:1.2}.u-mbs-0{margin-block-start:0!important}.c-article-header{font-family:Merriweather Sans,Helvetica Neue,Helvetica,Arial,sans-serif}.c-article-identifiers{color:#6f6f6f;display:flex;flex-wrap:wrap;font-size:1rem;line-height:1.3;list-style:none;margin:0 0 8px;padding:0}.c-article-identifiers__item{border-right:1px solid #6f6f6f;list-style:none;margin-right:8px;padding-right:8px}.c-article-identifiers__item:last-child{border-right:0;margin-right:0;padding-right:0}@media only screen and (min-width:876px){.c-article-title{font-size:1.875rem;line-height:1.2}}.c-article-author-list{display:inline;font-size:1rem;list-style:none;margin:0 8px 0 0;padding:0;width:100%}.c-article-author-list__item{display:inline;padding-right:0}.c-article-author-list__show-more{display:none;margin-right:4px}.c-article-author-list__button,.js .c-article-author-list__item--hide,.js .c-article-author-list__show-more{display:none}.js .c-article-author-list--long .c-article-author-list__show-more,.js .c-article-author-list--long+.c-article-author-list__button{display:inline}@media only screen and (max-width:767px){.js .c-article-author-list__item--hide-small-screen{display:none}.js .c-article-author-list--short .c-article-author-list__show-more,.js .c-article-author-list--short+.c-article-author-list__button{display:inline}}#uptodate-client,.js .c-article-author-list--expanded .c-article-author-list__show-more{display:none!important}.js .c-article-author-list--expanded .c-article-author-list__item--hide-small-screen{display:inline!important}.c-article-author-list__button,.c-button-author-list{background:#ebf1f5;border:4px solid #ebf1f5;border-radius:20px;color:#666;font-size:.875rem;line-height:1.4;padding:2px 11px 2px 8px;text-decoration:none}.c-article-author-list__button svg,.c-button-author-list svg{margin:1px 4px 0 0}.c-article-author-list__button:hover,.c-button-author-list:hover{background:#025e8d;border-color:transparent;color:#fff}.c-article-body .c-article-access-provider{padding:8px 16px}.c-article-body .c-article-access-provider,.c-notes{border:1px solid #d5d5d5;border-image:initial;border-left:none;border-right:none;margin:24px 0}.c-article-body .c-article-access-provider__text{color:#555}.c-article-body .c-article-access-provider__text,.c-notes__text{font-size:1rem;margin-bottom:0;padding-bottom:2px;padding-top:2px;text-align:center}.c-article-body .c-article-author-affiliation__address{color:inherit;font-weight:700;margin:0}.c-article-body .c-article-author-affiliation__authors-list{list-style:none;margin:0;padding:0}.c-article-body .c-article-author-affiliation__authors-item{display:inline;margin-left:0}.c-article-authors-search{margin-bottom:24px;margin-top:0}.c-article-authors-search__item,.c-article-authors-search__title{font-family:Merriweather Sans,Helvetica Neue,Helvetica,Arial,sans-serif}.c-article-authors-search__title{color:#626262;font-size:1.05rem;font-weight:700;margin:0;padding:0}.c-article-authors-search__item{font-size:1rem}.c-article-authors-search__text{margin:0}.c-code-block{border:1px solid #fff;font-family:monospace;margin:0 0 24px;padding:20px}.c-code-block__heading{font-weight:400;margin-bottom:16px}.c-code-block__line{display:block;overflow-wrap:break-word;white-space:pre-wrap}.c-article-share-box{font-family:Merriweather Sans,Helvetica Neue,Helvetica,Arial,sans-serif;margin-bottom:24px}.c-article-share-box__description{font-size:1rem;margin-bottom:8px}.c-article-share-box__no-sharelink-info{font-size:.813rem;font-weight:700;margin-bottom:24px;padding-top:4px}.c-article-share-box__only-read-input{border:1px solid #d5d5d5;box-sizing:content-box;display:inline-block;font-size:.875rem;font-weight:700;height:24px;margin-bottom:8px;padding:8px 10px}.c-article-share-box__additional-info{color:#626262;font-size:.813rem}.c-article-share-box__button{background:#fff;box-sizing:content-box;text-align:center}.c-article-share-box__button--link-like{background-color:transparent;border:0;color:#025e8d;cursor:pointer;font-size:.875rem;margin-bottom:8px;margin-left:10px}.c-article-associated-content__container .c-article-associated-content__collection-label{font-size:.875rem;line-height:1.4}.c-article-associated-content__container .c-article-associated-content__collection-title{line-height:1.3}.c-reading-companion{clear:both;min-height:389px}.c-reading-companion__figures-list,.c-reading-companion__references-list{list-style:none;min-height:389px;padding:0}.c-reading-companion__references-list--numeric{list-style:decimal inside}.c-reading-companion__figure-item{border-top:1px solid #d5d5d5;font-size:1rem;padding:16px 8px 16px 0}.c-reading-companion__figure-item:first-child{border-top:none;padding-top:8px}.c-reading-companion__reference-item{font-size:1rem}.c-reading-companion__reference-item:first-child{border-top:none}.c-reading-companion__reference-item a{word-break:break-word}.c-reading-companion__reference-citation{display:inline}.c-reading-companion__reference-links{font-size:.813rem;font-weight:700;list-style:none;margin:8px 0 0;padding:0;text-align:right}.c-reading-companion__reference-links>a{display:inline-block;padding-left:8px}.c-reading-companion__reference-links>a:first-child{display:inline-block;padding-left:0}.c-reading-companion__figure-title{display:block;font-size:1.25rem;font-weight:700;line-height:1.2;margin:0 0 8px}.c-reading-companion__figure-links{display:flex;justify-content:space-between;margin:8px 0 0}.c-reading-companion__figure-links>a{align-items:center;display:flex}.c-article-section__figure-caption{display:block;margin-bottom:8px;word-break:break-word}.c-article-section__figure .video,p.app-article-masthead__access--above-download{margin:0 0 16px}.c-article-section__figure-description{font-size:1rem}.c-article-section__figure-description>*{margin-bottom:0}.c-cod{display:block;font-size:1rem;width:100%}.c-cod__form{background:#ebf0f3}.c-cod__prompt{font-size:1.125rem;line-height:1.3;margin:0 0 24px}.c-cod__label{display:block;margin:0 0 4px}.c-cod__row{display:flex;margin:0 0 16px}.c-cod__row:last-child{margin:0}.c-cod__input{border:1px solid #d5d5d5;border-radius:2px;flex-shrink:0;margin:0;padding:13px}.c-cod__input--submit{background-color:#025e8d;border:1px solid #025e8d;color:#fff;flex-shrink:1;margin-left:8px;transition:background-color .2s ease-out 0s,color .2s ease-out 0s}.c-cod__input--submit-single{flex-basis:100%;flex-shrink:0;margin:0}.c-cod__input--submit:focus,.c-cod__input--submit:hover{background-color:#fff;color:#025e8d}.save-data .c-article-author-institutional-author__sub-division,.save-data .c-article-equation__number,.save-data .c-article-figure-description,.save-data .c-article-fullwidth-content,.save-data .c-article-main-column,.save-data .c-article-satellite-article-link,.save-data .c-article-satellite-subtitle,.save-data .c-article-table-container,.save-data .c-blockquote__body,.save-data .c-code-block__heading,.save-data .c-reading-companion__figure-title,.save-data .c-reading-companion__reference-citation,.save-data .c-site-messages--nature-briefing-email-variant .serif,.save-data .c-site-messages--nature-briefing-email-variant.serif,.save-data .serif,.save-data .u-serif,.save-data h1,.save-data h2,.save-data h3{font-family:Merriweather Sans,Helvetica Neue,Helvetica,Arial,sans-serif}.c-pdf-download__link{display:flex;flex:1 1 0%;padding:13px 24px}.c-pdf-download__link:hover{text-decoration:none}@media only screen and (min-width:768px){.c-context-bar--sticky .c-pdf-download__link{align-items:center;flex:1 1 183px}}@media only screen and (max-width:320px){.c-context-bar--sticky .c-pdf-download__link{padding:16px}}.c-article-body .c-article-recommendations-list,.c-book-body .c-article-recommendations-list{display:flex;flex-direction:row;gap:16px 16px;margin:0;max-width:100%;padding:16px 0 0}.c-article-body .c-article-recommendations-list__item,.c-book-body .c-article-recommendations-list__item{flex:1 1 0%}@media only screen and (max-width:767px){.c-article-body .c-article-recommendations-list,.c-book-body .c-article-recommendations-list{flex-direction:column}}.c-article-body .c-article-recommendations-card__authors{display:none;font-family:Merriweather Sans,Helvetica Neue,Helvetica,Arial,sans-serif;font-size:.875rem;line-height:1.5;margin:0 0 8px}@media only screen and (max-width:767px){.c-article-body .c-article-recommendations-card__authors{display:block;margin:0}}.c-article-body .c-article-history{margin-top:24px}.app-article-metrics-bar p{margin:0}.app-article-masthead{display:flex;flex-direction:column;gap:16px 16px;padding:16px 0 24px}.app-article-masthead__info{display:flex;flex-direction:column;flex-grow:1}.app-article-masthead__brand{border-top:1px solid hsla(0,0%,100%,.8);display:flex;flex-direction:column;flex-shrink:0;gap:8px 8px;min-height:96px;padding:16px 0 0}.app-article-masthead__brand img{border:1px solid #fff;border-radius:8px;box-shadow:0 4px 15px 0 hsla(0,0%,50%,.25);height:auto;left:0;position:absolute;width:72px}.app-article-masthead__journal-link{display:block;font-size:1.125rem;font-weight:700;margin:0 0 8px;max-width:400px;padding:0 0 0 88px;position:relative}.app-article-masthead__journal-title{-webkit-box-orient:vertical;-webkit-line-clamp:3;display:-webkit-box;overflow:hidden}.app-article-masthead__submission-link{align-items:center;display:flex;font-size:1rem;gap:4px 4px;margin:0 0 0 88px}.app-article-masthead__access{align-items:center;display:flex;flex-wrap:wrap;font-size:.875rem;font-weight:300;gap:4px 4px;margin:0}.app-article-masthead__buttons{display:flex;flex-flow:column wrap;gap:16px 16px}.app-article-masthead__access svg,.app-masthead--pastel .c-pdf-download .u-button--primary svg,.app-masthead--pastel .c-pdf-download .u-button--secondary svg,.c-context-bar--sticky .c-context-bar__container .c-pdf-download .u-button--primary svg,.c-context-bar--sticky .c-context-bar__container .c-pdf-download .u-button--secondary svg{fill:currentcolor}.app-article-masthead a{color:#fff}.app-masthead--pastel .c-pdf-download .u-button--primary,.c-context-bar--sticky .c-context-bar__container .c-pdf-download .u-button--primary{background-color:#025e8d;background-image:none;border:2px solid transparent;box-shadow:none;color:#fff;font-weight:700}.app-masthead--pastel .c-pdf-download .u-button--primary:visited,.c-context-bar--sticky .c-context-bar__container .c-pdf-download .u-button--primary:visited{color:#fff}.app-masthead--pastel .c-pdf-download .u-button--primary:hover,.c-context-bar--sticky .c-context-bar__container .c-pdf-download .u-button--primary:hover{text-decoration:none}.app-masthead--pastel .c-pdf-download .u-button--primary:focus,.c-context-bar--sticky .c-context-bar__container .c-pdf-download .u-button--primary:focus{border:4px solid #fc0;box-shadow:none;outline:0;text-decoration:none}.app-masthead--pastel .c-pdf-download .u-button--primary:focus,.app-masthead--pastel .c-pdf-download .u-button--primary:hover,.c-context-bar--sticky .c-context-bar__container .c-pdf-download .u-button--primary:focus,.c-context-bar--sticky .c-context-bar__container .c-pdf-download .u-button--primary:hover{background-color:#fff;background-image:none;color:#01324b}.app-masthead--pastel .c-pdf-download .u-button--primary:hover,.c-context-bar--sticky .c-context-bar__container .c-pdf-download .u-button--primary:hover{background:0 0;border:2px solid #025e8d;box-shadow:none;color:#025e8d}.app-masthead--pastel .c-pdf-download .u-button--secondary,.c-context-bar--sticky .c-context-bar__container .c-pdf-download .u-button--secondary{background:0 0;border:2px solid #025e8d;color:#025e8d;font-weight:700}.app-masthead--pastel .c-pdf-download .u-button--secondary:visited,.c-context-bar--sticky .c-context-bar__container .c-pdf-download .u-button--secondary:visited{color:#01324b}.app-masthead--pastel .c-pdf-download .u-button--secondary:hover,.c-context-bar--sticky .c-context-bar__container .c-pdf-download .u-button--secondary:hover{background-color:#01324b;background-color:#025e8d;border:2px solid transparent;box-shadow:none;color:#fff}.app-masthead--pastel .c-pdf-download .u-button--secondary:focus,.c-context-bar--sticky .c-context-bar__container .c-pdf-download .u-button--secondary:focus{background-color:#fff;background-image:none;border:4px solid #fc0;color:#01324b}@media only screen and (min-width:768px){.app-article-masthead{flex-direction:row;gap:64px 64px;padding:24px 0}.app-article-masthead__brand{border:0;padding:0}.app-article-masthead__brand img{height:auto;position:static;width:auto}.app-article-masthead__buttons{align-items:center;flex-direction:row;margin-top:auto}.app-article-masthead__journal-link{display:flex;flex-direction:column;gap:24px 24px;margin:0 0 8px;padding:0}.app-article-masthead__submission-link{margin:0}}@media only screen and (min-width:1024px){.app-article-masthead__brand{flex-basis:400px}}.app-article-masthead .c-article-identifiers{font-size:.875rem;font-weight:300;line-height:1;margin:0 0 8px;overflow:hidden;padding:0}.app-article-masthead .c-article-identifiers--cite-list{margin:0 0 16px}.app-article-masthead .c-article-identifiers *{color:#fff}.app-article-masthead .c-cod{display:none}.app-article-masthead .c-article-identifiers__item{border-left:1px solid #fff;border-right:0;margin:0 17px 8px -9px;padding:0 0 0 8px}.app-article-masthead .c-article-identifiers__item--cite{border-left:0}.app-article-metrics-bar{display:flex;flex-wrap:wrap;font-size:1rem;padding:16px 0 0;row-gap:24px}.app-article-metrics-bar__item{padding:0 16px 0 0}.app-article-metrics-bar__count{font-weight:700}.app-article-metrics-bar__label{font-weight:400;padding-left:4px}.app-article-metrics-bar__icon{height:auto;margin-right:4px;margin-top:-4px;width:auto}.app-article-metrics-bar__arrow-icon{margin:4px 0 0 4px}.app-article-metrics-bar a{color:#000}.app-article-metrics-bar .app-article-metrics-bar__item--metrics{padding-right:0}.app-overview-section .c-article-author-list,.app-overview-section__authors{line-height:2}.app-article-metrics-bar{margin-top:8px}.c-book-toc-pagination+.c-book-section__back-to-top{margin-top:0}.c-article-body .c-article-access-provider__text--chapter{color:#222;font-family:Merriweather Sans,Helvetica Neue,Helvetica,Arial,sans-serif;padding:20px 0}.c-article-body .c-article-access-provider__text--chapter svg.c-status-message__icon{fill:#003f8d;vertical-align:middle}.c-article-body-section__content--separator{padding-top:40px}.c-pdf-download__link{max-height:44px}.app-article-access .u-button--primary,.app-article-access .u-button--primary:visited{color:#fff}.c-article-sidebar{display:none}@media only screen and (min-width:1024px){.c-article-sidebar{display:block}}.c-cod__form{border-radius:12px}.c-cod__label{font-size:.875rem}.c-cod .c-status-message{align-items:center;justify-content:center;margin-bottom:16px;padding-bottom:16px}@media only screen and (min-width:1024px){.c-cod .c-status-message{align-items:inherit}}.c-cod .c-status-message__icon{margin-top:4px}.c-cod .c-cod__prompt{font-size:1rem;margin-bottom:16px}.c-article-body .app-article-access,.c-book-body .app-article-access{display:block}@media only screen and (min-width:1024px){.c-article-body .app-article-access,.c-book-body .app-article-access{display:none}}.c-article-body .app-card-service{margin-bottom:32px}@media only screen and (min-width:1024px){.c-article-body .app-card-service{display:none}}.app-article-access .buybox__buy .u-button--secondary,.app-article-access .u-button--primary,.c-cod__row .u-button--primary{background-color:#025e8d;border:2px solid #025e8d;box-shadow:none;font-size:1rem;font-weight:700;gap:8px 8px;justify-content:center;line-height:1.5;padding:8px 24px}.app-article-access .buybox__buy .u-button--secondary,.app-article-access .u-button--primary:hover,.c-cod__row .u-button--primary:hover{background-color:#fff;color:#025e8d}.app-article-access .buybox__buy .u-button--secondary:hover{background-color:#025e8d;color:#fff}.buybox__buy .c-notes__text{color:#666;font-size:.875rem;padding:0 16px 8px}.c-cod__input{flex-basis:auto;width:100%}.c-article-title{font-family:Merriweather Sans,Helvetica Neue,Helvetica,Arial,sans-serif;font-size:2.25rem;font-weight:700;line-height:1.2;margin:12px 0}.c-reading-companion__figure-item figure{margin:0}@media only screen and (min-width:768px){.c-article-title{margin:16px 0}}.app-article-access{border:1px solid #c5e0f4;border-radius:12px}.app-article-access__heading{border-bottom:1px solid #c5e0f4;font-family:Merriweather Sans,Helvetica Neue,Helvetica,Arial,sans-serif;font-size:1.125rem;font-weight:700;margin:0;padding:16px;text-align:center}.app-article-access .buybox__info svg{vertical-align:middle}.c-article-body .app-article-access p{margin-bottom:0}.app-article-access .buybox__info{font-family:Merriweather Sans,Helvetica Neue,Helvetica,Arial,sans-serif;font-size:1rem;margin:0}.app-article-access{margin:0 0 32px}@media only screen and (min-width:1024px){.app-article-access{margin:0 0 24px}}.c-status-message{font-size:1rem}.c-article-body{font-size:1.125rem}.c-article-body dl,.c-article-body ol,.c-article-body p,.c-article-body ul{margin-bottom:32px;margin-top:0}.c-article-access-provider__text:last-of-type,.c-article-body .c-notes__text:last-of-type{margin-bottom:0}.c-article-body ol p,.c-article-body ul p{margin-bottom:16px}.c-article-section__figure-caption{font-family:Merriweather Sans,Helvetica Neue,Helvetica,Arial,sans-serif}.c-reading-companion__figure-item{border-top-color:#c5e0f4}.c-reading-companion__sticky{max-width:400px}.c-article-section .c-article-section__figure-description>*{font-size:1rem;margin-bottom:16px}.c-reading-companion__reference-item{border-top:1px solid #d5d5d5;padding:16px 0}.c-reading-companion__reference-item:first-child{padding-top:0}.c-article-share-box__button,.js .c-article-authors-search__item .c-article-button{background:0 0;border:2px solid #025e8d;border-radius:32px;box-shadow:none;color:#025e8d;font-size:1rem;font-weight:700;line-height:1.5;margin:0;padding:8px 24px;transition:all .2s ease 0s}.c-article-authors-search__item .c-article-button{width:100%}.c-pdf-download .u-button{background-color:#fff;border:2px solid #fff;color:#01324b;justify-content:center}.c-context-bar__container .c-pdf-download .u-button svg,.c-pdf-download .u-button svg{fill:currentcolor}.c-pdf-download .u-button:visited{color:#01324b}.c-pdf-download .u-button:hover{border:4px solid #01324b;box-shadow:none}.c-pdf-download .u-button:focus,.c-pdf-download .u-button:hover{background-color:#01324b}.c-pdf-download .u-button:focus svg path,.c-pdf-download .u-button:hover svg path{fill:#fff}.c-context-bar__container .c-pdf-download .u-button{background-image:none;border:2px solid;color:#fff}.c-context-bar__container .c-pdf-download .u-button:visited{color:#fff}.c-context-bar__container .c-pdf-download .u-button:hover{text-decoration:none}.c-context-bar__container .c-pdf-download .u-button:focus{box-shadow:none;outline:0;text-decoration:none}.c-context-bar__container .c-pdf-download .u-button:focus,.c-context-bar__container .c-pdf-download .u-button:hover{background-color:#fff;background-image:none;color:#01324b}.c-context-bar__container .c-pdf-download .u-button:focus svg path,.c-context-bar__container .c-pdf-download .u-button:hover svg path{fill:#01324b}.c-context-bar__container .c-pdf-download .u-button,.c-pdf-download .u-button{box-shadow:none;font-size:1rem;font-weight:700;line-height:1.5;padding:8px 24px}.c-context-bar__container .c-pdf-download .u-button{background-color:#025e8d}.c-pdf-download .u-button:hover{border:2px solid #fff}.c-pdf-download .u-button:focus,.c-pdf-download .u-button:hover{background:0 0;box-shadow:none;color:#fff}.c-context-bar__container .c-pdf-download .u-button:hover{border:2px solid #025e8d;box-shadow:none;color:#025e8d}.c-context-bar__container .c-pdf-download .u-button:focus,.c-pdf-download .u-button:focus{border:2px solid #025e8d}.c-article-share-box__button:focus:focus,.c-article__pill-button:focus:focus,.c-context-bar__container .c-pdf-download .u-button:focus:focus,.c-pdf-download .u-button:focus:focus{outline:3px solid #08c;will-change:transform}.c-pdf-download__link .u-icon{padding-top:0}.c-bibliographic-information__column button{margin-bottom:16px}.c-article-body .c-article-author-affiliation__list p,.c-article-body .c-article-author-information__list p,figure{margin:0}.c-article-share-box__button{margin-right:16px}.c-status-message--boxed{border-radius:12px}.c-article-associated-content__collection-title{font-size:1rem}.app-card-service__description,.c-article-body .app-card-service__description{color:#222;margin-bottom:0;margin-top:8px}.app-article-access__subscriptions a,.app-article-access__subscriptions a:visited,.app-book-series-listing__item a,.app-book-series-listing__item a:hover,.app-book-series-listing__item a:visited,.c-article-author-list a,.c-article-author-list a:visited,.c-article-buy-box a,.c-article-buy-box a:visited,.c-article-peer-review a,.c-article-peer-review a:visited,.c-article-satellite-subtitle a,.c-article-satellite-subtitle a:visited,.c-breadcrumbs__link,.c-breadcrumbs__link:hover,.c-breadcrumbs__link:visited{color:#000}.c-article-author-list svg{height:24px;margin:0 0 0 6px;width:24px}.c-article-header{margin-bottom:32px}@media only screen and (min-width:876px){.js .c-ad--conditional{display:block}}.u-lazy-ad-wrapper{background-color:#fff;display:none;min-height:149px}@media only screen and (min-width:876px){.u-lazy-ad-wrapper{display:block}}p.c-ad__label{margin-bottom:4px}.c-ad--728x90{background-color:#fff;border-bottom:2px solid #cedbe0} } </style> <style>@media only print, only all and (prefers-color-scheme: no-preference), only all and (prefers-color-scheme: light), only all and (prefers-color-scheme: dark) { .eds-c-header__brand img{height:24px;width:203px}.app-article-masthead__journal-link img{height:93px;width:72px}@media only screen and (min-width:769px){.app-article-masthead__journal-link img{height:161px;width:122px}} } </style> <link rel="stylesheet" data-test="critical-css-handler" data-inline-css-source="critical-css" href=/oscar-static/app-springerlink/css/core-darwin-3c86549cfc.css media="print" onload="this.media='all';this.onload=null"> <link rel="stylesheet" data-test="critical-css-handler" data-inline-css-source="critical-css" href="/oscar-static/app-springerlink/css/enhanced-darwin-article-72ba046d97.css" media="print" onload="this.media='only print, only all and (prefers-color-scheme: no-preference), only all and (prefers-color-scheme: light), only all and (prefers-color-scheme: dark)';this.onload=null"> <script> window.dataLayer = [{"GA Key":"UA-26408784-1","DOI":"10.1007/978-1-0716-4108-8_22","Page":"protocol","page":{"attributes":{"environment":"live"}},"Country":"HK","japan":false,"doi":"10.1007-978-1-0716-4108-8_22","Keywords":"Zebrafish larvae, Viral infection, Grass carp reovirus, Innate immunity","kwrd":["Zebrafish_larvae","Viral_infection","Grass_carp_reovirus","Innate_immunity"],"Labs":"Y","ksg":"Krux.segments","kuid":"Krux.uid","Has Body":"Y","Features":[],"Open Access":"N","hasAccess":"N","bypassPaywall":"N","user":{"license":{"businessPartnerID":[],"businessPartnerIDString":""}},"Access Type":"no-access","Bpids":"","Bpnames":"","BPID":["1"],"VG Wort Identifier":"pw-vgzm.415900-10.1007-978-1-0716-4108-8","Full HTML":"N","session":{"authentication":{"loginStatus":"N"},"attributes":{"edition":"academic"}},"content":{"serial":{"eissn":"1940-6029","pissn":"1064-3745"},"book":{"doi":"10.1007/978-1-0716-4108-8","title":"Antiviral Innate Immunity","pisbn":"978-1-0716-4107-1","eisbn":"978-1-0716-4108-8","bookProductType":"ContributedVolume","seriesTitle":"Methods in Molecular Biology","seriesId":"7651"},"chapter":{"doi":"10.1007/978-1-0716-4108-8_22"},"type":"Protocol","category":{"pmc":{"primarySubject":"Biomedicine","primarySubjectCode":"SCB","secondarySubjects":{"1":"Immunology","2":"Virology"},"secondarySubjectCodes":{"1":"SCB14000","2":"SCB22003"}},"sucode":"SUCO11642"},"attributes":{"deliveryPlatform":"oscar"},"country":"HK","Has Preview":"N","subjectCodes":"SCB,SCB14000,SCB22003","PMC":["SCB","SCB14000","SCB22003"]},"Event Category":"Protocol","ConferenceSeriesId":"","productId":"9781071641088"}]; </script> <script> window.dataLayer.push({ ga4MeasurementId: 'G-B3E4QL2TPR', ga360TrackingId: 'UA-26408784-1', twitterId: 'o47a7', baiduId: 'aef3043f025ccf2305af8a194652d70b', ga4ServerUrl: 'https://collect.springer.com', imprint: 'springerlink', page: { attributes:{ featureFlags: [{ name: 'darwin-orion', active: true }, { name: 'chapter-books-recs', active: true }, { name: 'darwin-books', active: true }], darwinAvailable: true } } }); </script> <script data-test="gtm-head"> window.initGTM = function() { if (window.config.mustardcut) { (function (w, d, s, l, i) { w[l] = w[l] || []; w[l].push({'gtm.start': new Date().getTime(), event: 'gtm.js'}); var f = d.getElementsByTagName(s)[0], j = d.createElement(s), dl = l != 'dataLayer' ? '&l=' + l : ''; j.async = true; j.src = 'https://www.googletagmanager.com/gtm.js?id=' + i + dl; f.parentNode.insertBefore(j, f); })(window, document, 'script', 'dataLayer', 'GTM-MRVXSHQ'); } } </script> <script> (function (w, d, t) { function cc() { var h = w.location.hostname; var e = d.createElement(t), s = d.getElementsByTagName(t)[0]; if (h.indexOf('springer.com') > -1 && h.indexOf('biomedcentral.com') === -1 && h.indexOf('springeropen.com') === -1) { if (h.indexOf('link-qa.springer.com') > -1 || h.indexOf('test-www.springer.com') > -1) { e.src = 'https://cmp.springer.com/production_live/en/consent-bundle-17-52.js'; e.setAttribute('onload', "initGTM(window,document,'script','dataLayer','GTM-MRVXSHQ')"); } else { e.src = 'https://cmp.springer.com/production_live/en/consent-bundle-17-52.js'; e.setAttribute('onload', "initGTM(window,document,'script','dataLayer','GTM-MRVXSHQ')"); } } else if (h.indexOf('biomedcentral.com') > -1) { if (h.indexOf('biomedcentral.com.qa') > -1) { e.src = 'https://cmp.biomedcentral.com/production_live/en/consent-bundle-15-36.js'; e.setAttribute('onload', "initGTM(window,document,'script','dataLayer','GTM-MRVXSHQ')"); } else { e.src = 'https://cmp.biomedcentral.com/production_live/en/consent-bundle-15-36.js'; e.setAttribute('onload', "initGTM(window,document,'script','dataLayer','GTM-MRVXSHQ')"); } } else if (h.indexOf('springeropen.com') > -1) { if (h.indexOf('springeropen.com.qa') > -1) { e.src = 'https://cmp.springernature.com/production_live/en/consent-bundle-16-34.js'; e.setAttribute('onload', "initGTM(window,document,'script','dataLayer','GTM-MRVXSHQ')"); } else { e.src = 'https://cmp.springernature.com/production_live/en/consent-bundle-16-34.js'; e.setAttribute('onload', "initGTM(window,document,'script','dataLayer','GTM-MRVXSHQ')"); } } else if (h.indexOf('springernature.com') > -1) { if (h.indexOf('beta-qa.springernature.com') > -1) { e.src = 'https://cmp.springernature.com/production_live/en/consent-bundle-49-43.js'; e.setAttribute('onload', "initGTM(window,document,'script','dataLayer','GTM-NK22KLS')"); } else { e.src = 'https://cmp.springernature.com/production_live/en/consent-bundle-49-43.js'; e.setAttribute('onload', "initGTM(window,document,'script','dataLayer','GTM-NK22KLS')"); } } else { e.src = '/oscar-static/js/cookie-consent-es5-bundle-cb57c2c98a.js'; e.setAttribute('data-consent', h); } s.insertAdjacentElement('afterend', e); } cc(); })(window, document, 'script'); </script> <script> (function(w, d) { w.config = w.config || {}; w.config.mustardcut = false; if (w.matchMedia && w.matchMedia('only print, only all and (prefers-color-scheme: no-preference), only all and (prefers-color-scheme: light), only all and (prefers-color-scheme: dark)').matches) { w.config.mustardcut = true; d.classList.add('js'); d.classList.remove('grade-c'); d.classList.remove('no-js'); } })(window, document.documentElement); </script> <script> (function () { if ( typeof window.CustomEvent === "function" ) return false; function CustomEvent ( event, params ) { params = params || { bubbles: false, cancelable: false, detail: null }; var evt = document.createEvent( 'CustomEvent' ); evt.initCustomEvent( event, params.bubbles, params.cancelable, params.detail ); return evt; } CustomEvent.prototype = window.Event.prototype; window.CustomEvent = CustomEvent; })(); </script> <script class="js-entry"> if (window.config.mustardcut) { (function(w, d) { window.Component = {}; window.suppressShareButton = false; window.onArticlePage = true; var currentScript = d.currentScript || d.head.querySelector('script.js-entry'); function catchNoModuleSupport() { var scriptEl = d.createElement('script'); return (!('noModule' in scriptEl) && 'onbeforeload' in scriptEl) } var headScripts = [ {'src': '/oscar-static/js/polyfill-es5-bundle-572d4fec60.js', 'async': false} ]; var bodyScripts = [ {'src': '/oscar-static/js/global-article-es5-bundle-dad1690b0d.js', 'async': false, 'module': false}, {'src': '/oscar-static/js/global-article-es6-bundle-e7d03c4cb3.js', 'async': false, 'module': true} ]; function createScript(script) { var scriptEl = d.createElement('script'); scriptEl.src = script.src; scriptEl.async = script.async; if (script.module === true) { scriptEl.type = "module"; if (catchNoModuleSupport()) { scriptEl.src = ''; } } else if (script.module === false) { scriptEl.setAttribute('nomodule', true) } if (script.charset) { scriptEl.setAttribute('charset', script.charset); } return scriptEl; } for (var i = 0; i < headScripts.length; ++i) { var scriptEl = createScript(headScripts[i]); currentScript.parentNode.insertBefore(scriptEl, currentScript.nextSibling); } d.addEventListener('DOMContentLoaded', function() { for (var i = 0; i < bodyScripts.length; ++i) { var scriptEl = createScript(bodyScripts[i]); d.body.appendChild(scriptEl); } }); // Webfont repeat view var config = w.config; if (config && config.publisherBrand && sessionStorage.fontsLoaded === 'true') { d.documentElement.className += ' webfonts-loaded'; } })(window, document); } </script> <script data-src="https://cdn.optimizely.com/js/27195530232.js" data-cc-script="C03"></script> <link rel="canonical" href="https://link.springer.com/protocol/10.1007/978-1-0716-4108-8_22"/> <script type="application/ld+json">{"headline":"Zebrafish as a Model for Investigating Antiviral Innate Immunity","pageEnd":"236","pageStart":"221","image":"https://media.springernature.com/w153/springer-static/cover/book/978-1-0716-4108-8.jpg","genre":["Springer Protocols"],"isPartOf":{"name":"Antiviral Innate Immunity","isbn":["978-1-0716-4108-8","978-1-0716-4107-1"],"@type":"Book"},"publisher":{"name":"Springer US","logo":{"url":"https://www.springernature.com/app-sn/public/images/logo-springernature.png","@type":"ImageObject"},"@type":"Organization"},"author":[{"name":"Xiao Man Wu","affiliation":[{"name":"Institute of Hydrobiology, Chinese Academy of Sciences","address":{"name":"Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Si Yao Zheng","affiliation":[{"name":"Institute of Hydrobiology, Chinese Academy of Sciences","address":{"name":"Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China","@type":"PostalAddress"},"@type":"Organization"},{"name":"University of Chinese Academy of Sciences","address":{"name":"College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Ming Xian Chang","affiliation":[{"name":"Institute of Hydrobiology, Chinese Academy of Sciences","address":{"name":"Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China","@type":"PostalAddress"},"@type":"Organization"},{"name":"University of Chinese Academy of Sciences","address":{"name":"College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China","@type":"PostalAddress"},"@type":"Organization"}],"email":"mingxianchang@ihb.ac.cn","@type":"Person"}],"keywords":"Zebrafish larvae, Viral infection, Grass carp reovirus, Innate immunity","description":"Zebrafish is a widely used model organism in genetics, developmental biology, pathology, and immunology research. Due to their fast reproduction, large numbers, transparent early embryos, and high genetic conservation with the human genome, zebrafish have been used as a model for studying human and fish viral diseases. In particular, the ability to easily perform forward and reverse genetics and lacking a functional adaptive immune response during the early period of development establish the zebrafish as a favored option to assess the functional implication of specific genes in the antiviral innate immune response and the pathogenesis of viral diseases. In this chapter, we detail protocols for the antiviral innate immunity analysis using the zebrafish model, including the generation of gene-overexpression zebrafish, generation of gene-knockout zebrafish by clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology, methods of viral infection in zebrafish larvae, analyzing the expression of antiviral genes in zebrafish larvae using qRT-PCR, Western blotting and transcriptome sequencing, and in vivo antiviral assays. These experimental protocols provide effective references for studying the antiviral immune response in the zebrafish model.","datePublished":"2025","isAccessibleForFree":false,"hasPart":{"isAccessibleForFree":false,"cssSelector":".main-content","@type":"WebPageElement"},"@type":"ScholarlyArticle","@context":"https://schema.org"}</script> </head> <body class="shared-article-renderer">  <noscript data-test="gtm-body"> <iframe src="https://www.googletagmanager.com/ns.html?id=GTM-MRVXSHQ" height="0" width="0" style="display:none;visibility:hidden"></iframe> </noscript>  <div class="u-vh-full"> <a class="c-skip-link" href="#main-content">Skip to main content</a> <div class="u-hide u-show-following-ad"></div> <aside class="c-ad c-ad--728x90" data-test="springer-doubleclick-ad"> <div class="c-ad__inner"> <p class="c-ad__label">Advertisement</p> <div id="div-gpt-ad-LB1" data-pa11y-ignore data-gpt data-test="LB1-ad" data-gpt-unitpath="/270604982/springerlink/book/chapter" data-gpt-sizes="728x90" style="min-width:728px;min-height:90px" data-gpt-targeting="pos=LB1;"></div> </div> </aside> <div class="app-elements"> <header class="eds-c-header" data-eds-c-header> <div class="eds-c-header__container" data-eds-c-header-expander-anchor> <div class="eds-c-header__brand"> <a href="https://link.springer.com" data-test=springerlink-logo data-track="click_imprint_logo" data-track-context="unified header" data-track-action="click logo link" data-track-category="unified header" data-track-label="link" > <img src="/oscar-static/images/darwin/header/img/logo-springer-nature-link-3149409f62.svg" alt="Springer Nature Link"> </a> </div> <a class="c-header__link eds-c-header__link" id="identity-account-widget" href='https://idp.springer.com/auth/personal/springernature?redirect_uri=https://link.springer.com/protocol/10.1007/978-1-0716-4108-8_22?'><span class="eds-c-header__widget-fragment-title">Log in</span></a> </div> <nav class="eds-c-header__nav" aria-label="header navigation"> <div class="eds-c-header__nav-container"> <div class="eds-c-header__item eds-c-header__item--menu"> <a href="#eds-c-header-nav" class="eds-c-header__link" data-eds-c-header-expander> <svg class="eds-c-header__icon" width="24" height="24" aria-hidden="true" focusable="false"> <use xlink:href="#icon-eds-i-menu-medium"></use> </svg><span>Menu</span> </a> </div> <div class="eds-c-header__item eds-c-header__item--inline-links"> <a class="eds-c-header__link" href="https://link.springer.com/journals/" data-track="nav_find_a_journal" data-track-context="unified header" data-track-action="click find a journal" data-track-category="unified header" data-track-label="link" > Find a journal </a> <a class="eds-c-header__link" href="https://www.springernature.com/gp/authors" data-track="nav_how_to_publish" data-track-context="unified header" data-track-action="click publish with us link" data-track-category="unified header" data-track-label="link" > Publish with us </a> <a class="eds-c-header__link" href="https://link.springernature.com/home/" data-track="nav_track_your_research" data-track-context="unified header" data-track-action="click track your research" data-track-category="unified header" data-track-label="link" > Track your research </a> </div> <div class="eds-c-header__link-container"> <div class="eds-c-header__item eds-c-header__item--divider"> <a href="#eds-c-header-popup-search" class="eds-c-header__link" data-eds-c-header-expander data-eds-c-header-test-search-btn> <svg class="eds-c-header__icon" width="24" height="24" aria-hidden="true" focusable="false"> <use xlink:href="#icon-eds-i-search-medium"></use> </svg><span>Search</span> </a> </div> <div id="ecommerce-header-cart-icon-link" class="eds-c-header__item ecommerce-cart" style="display:inline-block"> <a class="eds-c-header__link" href="https://order.springer.com/public/cart" style="appearance:none;border:none;background:none;color:inherit;position:relative"> <svg id="eds-i-cart" class="eds-c-header__icon" xmlns="http://www.w3.org/2000/svg" height="24" width="24" viewBox="0 0 24 24" aria-hidden="true" focusable="false"> <path fill="currentColor" fill-rule="nonzero" d="M2 1a1 1 0 0 0 0 2l1.659.001 2.257 12.808a2.599 2.599 0 0 0 2.435 2.185l.167.004 9.976-.001a2.613 2.613 0 0 0 2.61-1.748l.03-.106 1.755-7.82.032-.107a2.546 2.546 0 0 0-.311-1.986l-.108-.157a2.604 2.604 0 0 0-2.197-1.076L6.042 5l-.56-3.17a1 1 0 0 0-.864-.82l-.12-.007L2.001 1ZM20.35 6.996a.63.63 0 0 1 .54.26.55.55 0 0 1 .082.505l-.028.1L19.2 15.63l-.022.05c-.094.177-.282.299-.526.317l-10.145.002a.61.61 0 0 1-.618-.515L6.394 6.999l13.955-.003ZM18 19a2 2 0 1 0 0 4 2 2 0 0 0 0-4ZM8 19a2 2 0 1 0 0 4 2 2 0 0 0 0-4Z"></path> </svg><span>Cart</span><span class="cart-info" style="display:none;position:absolute;top:10px;right:45px;background-color:#C65301;color:#fff;width:18px;height:18px;font-size:11px;border-radius:50%;line-height:17.5px;text-align:center"></span></a> <script>(function () { var exports = {}; if (window.fetch) { "use strict"; Object.defineProperty(exports, "__esModule", { value: true }); exports.headerWidgetClientInit = void 0; var headerWidgetClientInit = function (getCartInfo) { document.body.addEventListener("updatedCart", function () { updateCartIcon(); }, false); return updateCartIcon(); function updateCartIcon() { return getCartInfo() .then(function (res) { return res.json(); }) .then(refreshCartState) .catch(function (_) { }); } function refreshCartState(json) { var indicator = document.querySelector("#ecommerce-header-cart-icon-link .cart-info"); /* istanbul ignore else */ if (indicator && json.itemCount) { indicator.style.display = 'block'; indicator.textContent = json.itemCount > 9 ? '9+' : json.itemCount.toString(); var moreThanOneItem = json.itemCount > 1; indicator.setAttribute('title', "there ".concat(moreThanOneItem ? "are" : "is", " ").concat(json.itemCount, " item").concat(moreThanOneItem ? "s" : "", " in your cart")); } return json; } }; exports.headerWidgetClientInit = headerWidgetClientInit; headerWidgetClientInit( function () { return window.fetch("https://cart.springer.com/cart-info", { credentials: "include", headers: { Accept: "application/json" } }) } ) }})()</script> </div> </div> </div> </nav> </header> </div> <div class="app-masthead__colour-19--pastel app-masthead--pastel" id="main" data-track-component="chapter" data-test="masthead-component"> <section class="app-masthead " aria-label="book chapter masthead"> <div class="app-masthead__container"> <div class="app-article-masthead app-article-masthead--chapter u-sans-serif js-context-bar-sticky-point-masthead" data-track-component="chapter" data-test="masthead-component"> <div class="app-article-masthead__info"> <nav aria-label="breadcrumbs" data-test="breadcrumbs"> <ol class="c-breadcrumbs" itemscope itemtype="https://schema.org/BreadcrumbList"> <li class="c-breadcrumbs__item" id="breadcrumb0" itemprop="itemListElement" itemscope="" itemtype="https://schema.org/ListItem"> <a href="/" class="c-breadcrumbs__link" itemprop="item" data-track="click_breadcrumb" data-track-context="chapter page" data-track-category="Protocol" data-track-action="breadcrumbs" data-track-label="breadcrumb1"><span itemprop="name">Home</span></a><meta itemprop="position" content="1"> <svg class="c-breadcrumbs__chevron" role="img" aria-hidden="true" focusable="false" width="10" height="10" viewBox="0 0 10 10"> <path d="m5.96738168 4.70639573 2.39518594-2.41447274c.37913917-.38219212.98637524-.38972225 1.35419292-.01894278.37750606.38054586.37784436.99719163-.00013556 1.37821513l-4.03074001 4.06319683c-.37758093.38062133-.98937525.38100976-1.367372-.00003075l-4.03091981-4.06337806c-.37759778-.38063832-.38381821-.99150444-.01600053-1.3622839.37750607-.38054587.98772445-.38240057 1.37006824.00302197l2.39538588 2.4146743.96295325.98624457z" fill-rule="evenodd" transform="matrix(0 -1 1 0 0 10)"/> </svg> </li> <li class="c-breadcrumbs__item" id="breadcrumb1" itemprop="itemListElement" itemscope="" itemtype="https://schema.org/ListItem"> <a href="/book/10.1007/978-1-0716-4108-8" class="c-breadcrumbs__link" itemprop="item" data-track="click_breadcrumb" data-track-context="chapter page" data-track-category="Protocol" data-track-action="breadcrumbs" data-track-label="breadcrumb2"><span itemprop="name">Antiviral Innate Immunity</span></a><meta itemprop="position" content="2"> <svg class="c-breadcrumbs__chevron" role="img" aria-hidden="true" focusable="false" width="10" height="10" viewBox="0 0 10 10"> <path d="m5.96738168 4.70639573 2.39518594-2.41447274c.37913917-.38219212.98637524-.38972225 1.35419292-.01894278.37750606.38054586.37784436.99719163-.00013556 1.37821513l-4.03074001 4.06319683c-.37758093.38062133-.98937525.38100976-1.367372-.00003075l-4.03091981-4.06337806c-.37759778-.38063832-.38381821-.99150444-.01600053-1.3622839.37750607-.38054587.98772445-.38240057 1.37006824.00302197l2.39538588 2.4146743.96295325.98624457z" fill-rule="evenodd" transform="matrix(0 -1 1 0 0 10)"/> </svg> </li> <li class="c-breadcrumbs__item" id="breadcrumb2" itemprop="itemListElement" itemscope="" itemtype="https://schema.org/ListItem"> <span itemprop="name">Protocol</span><meta itemprop="position" content="3"> </li> </ol> </nav> <h1 class="c-article-title" data-test="chapter-title" data-chapter-title="">Zebrafish as a Model for Investigating Antiviral Innate Immunity</h1> <ul class="c-article-identifiers"> <li class="c-article-identifiers__item" data-test="article-category">Protocol</li> <li class="c-article-identifiers__item">First Online: <time datetime="2024-08-28">28 August 2024</time></li> </ul> <ul class="c-article-identifiers c-article-identifiers--cite-list"> <li class="c-article-identifiers__item"> <span class="c-chapter-book-details__meta"> pp 221–236</span> </li> <li class="c-article-identifiers__item c-article-identifiers__item--cite"> <a href="#citeas" data-track="click" data-track-action="cite this chapter" data-track-category="chapter body" data-track-label="link">Cite this protocol</a> </li> </ul> <div class="app-article-masthead__buttons" data-track-context="masthead"> </div> </div> <div class="app-article-masthead__brand app-article-masthead__brand--no-border app-article-masthead__conference-link"> <a href="/book/10.1007/978-1-0716-4108-8" class="app-article-masthead__conference-link app-article-masthead__journal-link" data-track="click" data-track-action="book homepage" data-track-label="link"> <picture> <source type="image/webp" media="(min-width: 768px)" width="120" height="182" srcset="https://media.springernature.com/w120/springer-static/cover-hires/book/978-1-0716-4108-8?as=webp, https://media.springernature.com/w316/springer-static/cover-hires/book/978-1-0716-4108-8?as=webp 2x"> <img width="72" height="109" src="https://media.springernature.com/w72/springer-static/cover-hires/book/978-1-0716-4108-8?as=webp" srcset="https://media.springernature.com/w144/springer-static/cover-hires/book/978-1-0716-4108-8?as=webp 2x" alt=""> </picture> <span class="app-article-masthead__journal-title ">Antiviral Innate Immunity</span> </a> <span class="app-article-masthead__conference-info"></span> </div> </div> </div> </section> </div> <div class="c-article-main u-container u-mt-24 u-mb-32 l-with-sidebar" id="main-content" data-component="article-container"> <main class="js-main-column u-serif c-chapter-body" data-track-component="chapter"> <article lang="en"> <div class="c-article-header"> <header> <div class="app-overview-section"> <ul class="c-article-author-list c-article-author-list--short" data-test="authors-list" data-component-authors-activator="authors-list"><li class="c-article-author-list__item"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Xiao_Man-Wu" data-author-popup="auth-Xiao_Man-Wu">Xiao Man Wu</a><sup class="u-js-hide"><a href="#Aff3">3</a></sup>, </li><li class="c-article-author-list__item"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Si_Yao-Zheng" data-author-popup="auth-Si_Yao-Zheng">Si Yao Zheng</a><sup class="u-js-hide"><a href="#Aff3">3</a>,<a href="#Aff4">4</a></sup> & </li><li class="c-article-author-list__item"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Ming_Xian-Chang" data-author-popup="auth-Ming_Xian-Chang" data-corresp-id="c1">Ming Xian Chang<svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-mail-medium"></use></svg></a><sup class="u-js-hide"><a href="#Aff3">3</a>,<a href="#Aff4">4</a></sup> </li></ul> <div class="app-overview-section__separator app-overview-section__book-series"> <div class="app-book-series-listing"> <div> <svg class="app-book-series-listing__icon" width="24" height="24" aria-hidden="true" focusable="false"><use href="#icon-eds-i-book-series-medium"></use></svg> </div> <div> <p data-test="series-link"> <span class="app-book-series-listing__description">Part of the book series:</span> <a href="https://www.springer.com/series/7651" data-track="click" data-track-action="open book series" data-track-label="link">Methods in Molecular Biology</a> ((MIMB,volume 2854)) </p> </div> </div> </div> <div class="app-overview-section__separator" data-test="article-metrics"> <div id="altmetric-container"> <ul class="app-article-metrics-bar u-list-reset" data-test="article-metrics"> <li class="app-article-metrics-bar__item" data-test="access-count"> <p class="app-article-metrics-bar__count"><svg class="u-icon app-article-metrics-bar__icon" width="24" height="24" aria-hidden="true" focusable="false"> <use xlink:href="#icon-eds-i-accesses-medium"></use> </svg>461 <span class="app-article-metrics-bar__label">Accesses</span></p> </li> </ul> </div> </div> </div> </header> </div> <div data-article-body="true" data-track-component="chapter body" class="c-article-body"> <section aria-labelledby="Abs1" data-title="Abstract" lang="en"><div class="c-article-section" id="Abs1-section"><h2 id="Abs1" class="c-article-section__title js-section-title js-c-reading-companion-sections-item"><span class="c-article-section__title-number"> </span>Abstract</h2><div class="c-article-section__content" id="Abs1-content"><p>Zebrafish is a widely used model organism in genetics, developmental biology, pathology, and immunology research. Due to their fast reproduction, large numbers, transparent early embryos, and high genetic conservation with the human genome, zebrafish have been used as a model for studying human and fish viral diseases. In particular, the ability to easily perform forward and reverse genetics and lacking a functional adaptive immune response during the early period of development establish the zebrafish as a favored option to assess the functional implication of specific genes in the antiviral innate immune response and the pathogenesis of viral diseases. In this chapter, we detail protocols for the antiviral innate immunity analysis using the zebrafish model, including the generation of gene-overexpression zebrafish, generation of gene-knockout zebrafish by clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology, methods of viral infection in zebrafish larvae, analyzing the expression of antiviral genes in zebrafish larvae using qRT-PCR, Western blotting and transcriptome sequencing, and in vivo antiviral assays. These experimental protocols provide effective references for studying the antiviral immune response in the zebrafish model.</p></div></div></section> <div class="c-notes"> <p class="c-notes__text c-status-message--info"> <svg width="24" height="24" focusable="false" role="img" aria-hidden="true" class="c-status-message__icon"> <use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-info-filled-medium"></use> </svg> This is a preview of subscription content, <a id="test-login-banner-link" href="//wayf.springernature.com?redirect_uri=https%3A%2F%2Flink.springer.com%2Fprotocol%2F10.1007%2F978-1-0716-4108-8_22%3Ferror%3Dcookies_not_supported%26code%3Db7045d23-fc9f-49e9-b549-6a41c1f97f27" data-track="click" data-track-action="login" data-track-label="link">log in via an institution</a> <svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon c-external-link__icon"> <use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-external-link-small"></use> </svg> to check access. </p> </div> <div data-test="access-chapter-mobile" class="app-article-access"> <h2 class="app-article-access__heading">Access this chapter</h2> <div class="u-ma-16 u-clear-both"> <a href="//wayf.springernature.com?redirect_uri=https%3A%2F%2Flink.springer.com%2Fprotocol%2F10.1007%2F978-1-0716-4108-8_22%3Ferror%3Dcookies_not_supported%26code%3Db7045d23-fc9f-49e9-b549-6a41c1f97f27" class="u-button u-button--full-width u-button--primary u-justify-content-space-between c-pdf-download__link" data-track="click" data-track-action="institution access" data-track-label="button"> <span data-test="access-via-institution">Log in via an institution</span> <svg aria-hidden="true" focusable="false" width="16" height="16" class="u-icon"> <use xlink:href="#icon-eds-i-arrow-right-medium"></use> </svg> </a> </div> <div data-test="buy-box-mobile" class="u-mb-24"> <div class="sprcom-buybox-darwin sprcom-buybox-darwin-b buybox" id="sprcom-buybox-darwin-b"> <div> <div class="c-springer-plus"> <h2 class="springer-plus-heading">Subscribe and save</h2> <div class="springer-plus"> <div class="springer-plus-headline"> <div class="springer-plus-title"> <svg aria-hidden="true" focusable="false" width="16" height="16" class="u-icon"> <use xlink:href="#icon-eds-i-check-filled-medium"></use> </svg><span>Springer+ Basic</span> </div> <div class="dd price-amount-springer-plus"> €32.70 /Month </div> </div> <ul class="buying-option-usps"> <li>Get 10 units per month</li> <li>Download Article/Chapter or eBook</li> <li>1 Unit = 1 Article or 1 Chapter</li> <li>Cancel anytime</li> </ul><a href="https://link.springer.com/product/springer-plus" class="u-button u-button--full-width u-button--secondary" id="btn-subscribe-springerPlus" data-track="click||click_springer_subscribe" data-track-context="buy box"> <span>Subscribe now </span> <svg aria-hidden="true" focusable="false" width="16" height="16" class="u-icon"> <use xlink:href="#icon-eds-i-arrow-right-medium"></use> </svg></a> </div> <h2 class="springer-plus-heading-end">Buy Now</h2> </div> <div class="c-box"> <div class="buying-options"> <div class="buying-option expanded"> <dl class="buying-option-price"> <dt> <svg width="24" height="24" xmlns="http://www.w3.org/2000/svg" aria-hidden="true" focusable="false"> <path d="M11.782 11L9.3 8.518c-.393-.392-.4-1.022-.02-1.403a1.001 1.001 0 011.417 0l4.176 4.177a1.001 1.001 0 010 1.416l-4.176 4.177a.991.991 0 01-1.4.016 1 1 0 01.003-1.42L11.782 13l1.013-.998L11.782 11z" fill="#666" fill-rule="evenodd"></path> </svg> Protocol </dt> <dd class="price-amount"> <div data-test-id="test-chapter-price" class="buybox__price"> EUR 44.95 </div> </dd> <dd class="price-info"> Price includes VAT (Hong Kong/P.R.China) </dd> </dl> <form class="buying-option-form" action="https://order.springer.com/public/cart" method="post"> <input type="hidden" name="type" value="protocol"> <input type="hidden" name="doi" value="10.1007/978-1-0716-4108-8_22"> <input type="hidden" name="isxn" value="978-1-0716-4108-8"> <input type="hidden" name="contenttitle" value="Zebrafish as a Model for Investigating Antiviral Innate Immunity"> <input type="hidden" name="copyrightyear" value="2025"> <input type="hidden" name="year" value="2024"> <input type="hidden" name="authors" value="Xiao Man Wu, Si Yao Zheng, Ming Xian Chang"> <input type="hidden" name="title" value="Antiviral Innate Immunity"> <input type="hidden" name="mac" value="de422a41bdb749b7a4b08cd933940f3d"> <ul class="buying-option-usps"> <li>Available as PDF</li> <li>Read on any device</li> <li>Instant download</li> <li>Own it forever</li> </ul> <button type="submit" class="u-button u-button--full-width u-button--primary u-button--xsmall" value="Submit" data-track="click" data-track-prefer="click" data-track-action="buy pdf" data-track-label="buy chapter action" onclick="dataLayer.push({"event":"addToCart","ecommerce":{"currencyCode":"EUR","add":{"products":[{"name":"Zebrafish as a Model for Investigating Antiviral Innate Immunity","id":"10.1007/978-1-0716-4108-8_22","price":44.95,"brand":"Springer US","category":"Innate Immunity","variant":"ppv-protocol","quantity":1}]}}});">Buy Protocol <svg aria-hidden="true" focusable="false" width="16" height="16" class="u-icon"> <use xlink:href="#icon-eds-i-arrow-right-medium"></use> </svg></button> </form> </div> <div class="buying-option expanded"> <dl class="buying-option-price"> <dt> <svg width="24" height="24" xmlns="http://www.w3.org/2000/svg" aria-hidden="true" focusable="false"> <path d="M11.782 11L9.3 8.518c-.393-.392-.4-1.022-.02-1.403a1.001 1.001 0 011.417 0l4.176 4.177a1.001 1.001 0 010 1.416l-4.176 4.177a.991.991 0 01-1.4.016 1 1 0 01.003-1.42L11.782 13l1.013-.998L11.782 11z" fill="#666" fill-rule="evenodd"></path> </svg> eBook </dt> <dd class="price-amount"> EUR 171.19 </dd> <dd class="price-info"> Price includes VAT (Hong Kong/P.R.China) </dd> </dl> <form class="buying-option-form" action="https://order.springer.com/public/cart" method="post"> <input type="hidden" name="type" value="ebook"> <input type="hidden" name="doi" value="10.1007/978-1-0716-4108-8"> <input type="hidden" name="isxn" value="978-1-0716-4108-8"> <input type="hidden" name="contenttitle" value="Antiviral Innate Immunity"> <input type="hidden" name="mac" value="950e5c903ca87eceec05dfe3573d969e"> <ul class="buying-option-usps"> <li>Available as EPUB and PDF</li> <li>Read on any device</li> <li>Instant download</li> <li>Own it forever</li> </ul> <button type="submit" class="u-button u-button--full-width u-button--primary u-button--xsmall" value="Submit" data-track="click" data-track-prefer="click" data-track-label="buy ebook" onclick="dataLayer.push({"event":"addToCart","ecommerce":{"currencyCode":"EUR","add":{"products":[{"name":"Antiviral Innate Immunity","id":"978-1-0716-4108-8","price":149.99,"brand":"Springer US","category":"Innate Immunity","variant":"ebo","quantity":1}]}}});">Buy eBook <svg aria-hidden="true" focusable="false" width="16" height="16" class="u-icon"> <use xlink:href="#icon-eds-i-arrow-right-medium"></use> </svg></button> </form> </div> <div class="buying-option expanded"> <dl class="buying-option-price"> <dt> <svg width="24" height="24" xmlns="http://www.w3.org/2000/svg" aria-hidden="true" focusable="false"> <path d="M11.782 11L9.3 8.518c-.393-.392-.4-1.022-.02-1.403a1.001 1.001 0 011.417 0l4.176 4.177a1.001 1.001 0 010 1.416l-4.176 4.177a.991.991 0 01-1.4.016 1 1 0 01.003-1.42L11.782 13l1.013-.998L11.782 11z" fill="#666" fill-rule="evenodd"></path> </svg> Hardcover Book </dt> <dd class="price-amount"> EUR 219.99 </dd> <dd class="price-info"> Price excludes VAT (Hong Kong/P.R.China) </dd> </dl> <form class="buying-option-form" action="https://order.springer.com/public/cart" method="post"> <input type="hidden" name="type" value="book"> <input type="hidden" name="doi" value="10.1007/978-1-0716-4108-8"> <input type="hidden" name="isxn" value="978-1-0716-4107-1"> <input type="hidden" name="contenttitle" value="Antiviral Innate Immunity"> <input type="hidden" name="mac" value="c0f426b571edd1d0e4fb7c0fc8cb93f9"> <ul class="buying-option-usps"> <li>Durable hardcover edition</li> <li>Dispatched in 3 to 5 business days</li> <li>Free shipping worldwide - <a href="https://support.springernature.com/en/support/solutions/articles/6000233448-coronavirus-disease-covid-19-delivery-information" target="_blank">see info</a></li> </ul> <button type="submit" class="u-button u-button--full-width u-button--primary u-button--xsmall" value="Submit" data-track="click" data-track-prefer="click" data-track-label="buy hardcover" onclick="dataLayer.push({"event":"addToCart","ecommerce":{"currencyCode":"EUR","add":{"products":[{"name":"Antiviral Innate Immunity","id":"978-1-0716-4107-1","price":219.99,"brand":"Springer US","category":"Innate Immunity","variant":"print","quantity":1}]}}});">Buy Hardcover Book <svg aria-hidden="true" focusable="false" width="16" height="16" class="u-icon"> <use xlink:href="#icon-eds-i-arrow-right-medium"></use> </svg></button> </form> </div> </div> </div> <div class="buybox-tax-info"> <p class="c-notes__text tax-info">Tax calculation will be finalised at checkout</p> <p class="c-notes__text buybox-additional-info">Purchases are for personal use only</p> </div> </div> <style> .c-springer-plus { display: none; } .springer-plus { background-color: #EBF6FF; padding: 16px; font-family: "Merriweather Sans", "Helvetica Neue", Helvetica, Arial, sans-serif; } .springer-plus-headline { display: flex; justify-content: space-between; } .springer-plus-heading { border-bottom: 1px solid #c5e0f4; border-top: 1px solid #c5e0f4; font-family: "Merriweather Sans", "Helvetica Neue", Helvetica, Arial, sans-serif; font-size: 1.125rem; text-align: center; font-weight: 700; padding: 16px; margin: 0; } .springer-plus-heading-end { border-top: 1px solid #c5e0f4; font-family: "Merriweather Sans", "Helvetica Neue", Helvetica, Arial, sans-serif; font-size: 1.125rem; text-align: center; font-weight: 700; padding: 16px; margin: 0; } .springer-plus-title { display: flex; align-items: center; } .springer-plus-title span { margin-left: 8px; } .sprcom-buybox-darwin-b .springer-plus a { color: #025e8d; font-size: 16px; background-color: #fff; border: 1px solid #025e8d; font-weight: 700; max-height: 44px; } .sprcom-buybox-darwin-b .springer-plus a span { margin-right: 8px; } .sprcom-buybox-darwin-b .springer-plus a:hover { color: #fff; background-color: #025e8d; border: 4px solid #025e8d; box-shadow: none; font-weight: 700; } .sprcom-buybox-darwin-b .springer-plus a:visited { color: #025e8d; } .sprcom-buybox-darwin-b .springer-plus a:visited:hover { color: #fff; } .sprcom-buybox-darwin-b .springer-plus .buying-option-usps { color: #555; font-size: 1rem; line-height: 1.6; margin: 0; padding-left: 0; list-style: none; padding-top: 16px; padding-bottom: 24px; border-top: 0; } /* end of springer-plus */ .sprcom-buybox-darwin-b > div { flex-grow: 1; width: 100%; } .sprcom-buybox-darwin-b .buying-options { display: flex; flex-wrap: wrap; margin-top: 0; } .sprcom-buybox-darwin-b .buying-options > * { background-color: #F0F7FC; flex-grow: 1; flex-basis: auto; width: 360px; display: flex; flex-direction: column; justify-content: space-between; border-bottom: 1px solid #cedbe0; border-top: 1px solid #cedbe0; margin-top: -1px; } .sprcom-buybox-darwin-b dt { align-items: center; display: flex; font-weight: 700; margin-left: -10px; } .sprcom-buybox-darwin-b .buying-option-form { padding: 0 16px 16px; } .sprcom-buybox-darwin-b .buying-option-form button { gap: 8px; margin: 20px 0px; } .sprcom-buybox-darwin-b .buying-option-price { align-items: center; display: flex; flex-wrap: wrap; font-size: 1rem; line-height: 1.4; user-select: none; cursor: pointer; padding: 24px; margin: 0; } .sprcom-buybox-darwin-b .buying-option-price:focus { outline: 4px solid #08c; z-index: 1; } .sprcom-buybox-darwin-b .buying-option-price dt, .sprcom-buybox-darwin-b .buying-option-price dt svg path { color: #025e8d; fill: #025e8d; } .sprcom-buybox-darwin-b .buying-option-price dt, .sprcom-buybox-darwin-b .buying-option-price dd { flex-grow: 1; } .sprcom-buybox-darwin-b .buying-option-price dt svg { height: auto; min-width: 24px; margin-right: 12px; } .sprcom-buybox-darwin-b .buying-option-price .price-info { color: #555; font-size: .875rem; text-align: right; width: 100%; } .sprcom-buybox-darwin-b .buying-option-price .price-amount { color: #555; text-align: right; font-weight: 600; } .sprcom-buybox-darwin-b .buying-option-price .price-amount-without-discount { color: #c40606; text-decoration: line-through; width: 100%; } .sprcom-buybox-darwin-b .buying-option-price .price-type { font-size: 40%; margin-left: 8px; } .sprcom-buybox-darwin-b .buying-option-usps { color: #555; font-size: 1rem; line-height: 1.6; margin: 0; padding-left: 0; list-style: none; padding-top: 16px; border-top: 1px solid #f0f0f0; } .sprcom-buybox-darwin-b .buying-option-usps > li { position: relative; padding-left: 26px; } .sprcom-buybox-darwin-b .buying-option-usps > li::before { position: absolute; content: ""; left: 0; top: calc(0.8em - 5px); background-image: url("data:image/svg+xml,%3Csvg viewBox='0 0 100 100' xmlns='http://www.w3.org/2000/svg' fill='%230070A8'%3E%3Ccircle cx='50' cy='50' r='50'/%3E%3C/svg%3E"); width: 10px; height: 10px; } .sprcom-buybox-darwin-b .buying-option-usps > li:not(:first-child) { margin-top: 4px; } .sprcom-buybox-darwin-b .buying-options > .expanded { background-color: #fff; } .sprcom-buybox-darwin-b dl { } .sprcom-buybox-darwin-b a:visited { color: #004b83; } .sprcom-buybox-darwin-b [aria-expanded=false] svg { transform: rotate(90deg) scale(1.5); } .sprcom-buybox-darwin-b [aria-expanded=true] svg { transform: rotate(270deg) scale(1.5); } .sprcom-buybox-darwin-b dt { align-items: center; display: flex; } .sprcom-buybox-darwin-b style { display: none; } .sprcom-buybox-darwin-b .buybox-tax-info { text-align: center; padding: 16px; } .sprcom-buybox-darwin-b .tax-info, .sprcom-buybox-darwin-b .buybox-additional-info { font-size: .875rem; } .sprcom-buybox-darwin-b .buybox-additional-info { font-weight: 600; } .sprcom-buybox-darwin-b .u-button--primary.u-button--xsmall { font-size: .875rem; padding: 2px 8px; } </style> <script> ;(function () { var timestamp = Date.now() document.write('<div data-id="id_'+ timestamp +'"></div>') var buybox = document.querySelector("[data-id=id_"+ timestamp +"]").parentNode var buyingOptions = buybox.querySelectorAll(".buying-option") ;[].slice.call(buyingOptions).forEach(initCollapsibles) // springerPlus roll out 10% starts here var springerPlusGroup = setLocalStorageSpringerPlus(); var rollOutSpringerPlus = springerPlusGroup === "B" function setLocalStorageSpringerPlus() { var selectUserKey = "springerPlusRollOut"; var springerPlusGroup = "X"; if (!window.localStorage) return springerPlusGroup; try { var selectUserValue = window.localStorage.getItem(selectUserKey) springerPlusGroup = selectUserValue || randomDistributionSpringerPlus(selectUserKey) } catch (err) { console.log(err) } return springerPlusGroup; } function randomDistributionSpringerPlus(selectUserKey) { var randomGroup = Math.random() < 0.9 ? "A" : "B" window.localStorage.setItem(selectUserKey, randomGroup) return randomGroup } if (rollOutSpringerPlus) { revealSpringerPlus(); } function revealSpringerPlus() { if(buybox) { document.querySelectorAll(".c-springer-plus").forEach(function(node) { node.style.display = "block" }) } } //springerPlus ends here var buyboxMaxSingleColumnWidth = 480 function initCollapsibles(subscription, index) { var toggle = subscription.querySelector(".buying-option-price") subscription.classList.remove("expanded") var form = subscription.querySelector(".buying-option-form") var priceInfo = subscription.querySelector(".price-info") var buyingOption = toggle.parentElement if (toggle && form && priceInfo) { toggle.setAttribute("role", "button") toggle.setAttribute("tabindex", "0") toggle.addEventListener("click", function (event) { var expandedBuyingOptions = buybox.querySelectorAll(".buying-option.expanded") var buyboxWidth = buybox.offsetWidth ;[].slice.call(expandedBuyingOptions).forEach(function(option) { if (buyboxWidth <= buyboxMaxSingleColumnWidth && option != buyingOption) { hideBuyingOption(option) } }) var expanded = toggle.getAttribute("aria-expanded") === "true" || false toggle.setAttribute("aria-expanded", !expanded) form.hidden = expanded if (!expanded) { buyingOption.classList.add("expanded") } else { buyingOption.classList.remove("expanded") } priceInfo.hidden = expanded }, false) } } function hideBuyingOption(buyingOption) { var toggle = buyingOption.querySelector(".buying-option-price") var form = buyingOption.querySelector(".buying-option-form") var priceInfo = buyingOption.querySelector(".price-info") toggle.setAttribute("aria-expanded", false) form.hidden = true buyingOption.classList.remove("expanded") priceInfo.hidden = true } function initKeyControls() { document.addEventListener("keydown", function (event) { if (document.activeElement.classList.contains("buying-option-price") && (event.code === "Space" || event.code === "Enter")) { if (document.activeElement) { event.preventDefault() document.activeElement.click() } } }, false) } function initialStateOpen() { var buyboxWidth = buybox.offsetWidth ;[].slice.call(buybox.querySelectorAll(".buying-option")).forEach(function (option, index) { var toggle = option.querySelector(".buying-option-price") var form = option.querySelector(".buying-option-form") var priceInfo = option.querySelector(".price-info") if (buyboxWidth > buyboxMaxSingleColumnWidth) { toggle.click() } else { if (index === 0) { toggle.click() } else { toggle.setAttribute("aria-expanded", "false") form.hidden = "hidden" priceInfo.hidden = "hidden" } } }) } initialStateOpen() if (window.buyboxInitialised) return window.buyboxInitialised = true initKeyControls() })() </script> <script> ;(function () { if (document.cookie.indexOf("feature-monetise-subscriptions-display-springer-plus") > -1) { document.querySelectorAll(".c-springer-plus").forEach(function(node) { node.style.display = "block" }) } })() </script> </div> </div> <div class="app-article-access__subscriptions"> <p><a href="https://www.springernature.com/gp/librarians/licensing/agc/ebooks">Institutional subscriptions <svg aria-hidden="true" focusable="false" width="24" height="24" class="u-icon"> <use xlink:href="#icon-eds-i-arrow-right-medium"></use> </svg> </a></p> </div> </div> <div data-test="cobranding-download"> </div> <section aria-labelledby="inline-recommendations" data-title="Inline Recommendations" class="c-article-recommendations" data-track-component="inline-recommendations"> <h3 class="c-article-recommendations-title" id="inline-recommendations">Similar content being viewed by others</h3> <div class="c-article-recommendations-list"> <div class="c-article-recommendations-list__item"> <article class="c-article-recommendations-card" itemscope itemtype="http://schema.org/ScholarlyArticle"> <div class="c-article-recommendations-card__img"><img src="https://media.springernature.com/w92h120/springer-static/cover-hires/book/978-1-4939-7237-1?as=webp" loading="lazy" alt=""></div> <div class="c-article-recommendations-card__main"> <h3 class="c-article-recommendations-card__heading" itemprop="name headline"> <a class="c-article-recommendations-card__link" itemprop="url" href="https://link.springer.com/10.1007/978-1-4939-7237-1_2?fromPaywallRec=true" data-track="select_recommendations_1" data-track-context="inline recommendations" data-track-action="click recommendations inline - 1" data-track-label="10.1007/978-1-4939-7237-1_2">Zebrafish as a Model for the Study of Host-Virus Interactions </a> </h3> <div class="c-article-meta-recommendations" data-test="recommendation-info"> <span class="c-article-meta-recommendations__item-type">Chapter</span> <span class="c-article-meta-recommendations__date">© 2017</span> </div> </div> </article> </div> <div class="c-article-recommendations-list__item"> <article class="c-article-recommendations-card" itemscope itemtype="http://schema.org/ScholarlyArticle"> <div class="c-article-recommendations-card__img"><img src="https://media.springernature.com/w92h120/springer-static/cover-hires/book/978-1-4939-1396-1?as=webp" loading="lazy" alt=""></div> <div class="c-article-recommendations-card__main"> <h3 class="c-article-recommendations-card__heading" itemprop="name headline"> <a class="c-article-recommendations-card__link" itemprop="url" href="https://link.springer.com/10.1007/978-1-4939-1396-1_22?fromPaywallRec=true" data-track="select_recommendations_2" data-track-context="inline recommendations" data-track-action="click recommendations inline - 2" data-track-label="10.1007/978-1-4939-1396-1_22">Manipulating Galectin Expression in Zebrafish (Danio rerio) </a> </h3> <div class="c-article-meta-recommendations" data-test="recommendation-info"> <span class="c-article-meta-recommendations__item-type">Chapter</span> <span class="c-article-meta-recommendations__date">© 2015</span> </div> </div> </article> </div> <div class="c-article-recommendations-list__item"> <article class="c-article-recommendations-card" itemscope itemtype="http://schema.org/ScholarlyArticle"> <div class="c-article-recommendations-card__img"><img src="https://media.springernature.com/w92h120/springer-static/cover-hires/book/978-1-4939-1261-2?as=webp" loading="lazy" alt=""></div> <div class="c-article-recommendations-card__main"> <h3 class="c-article-recommendations-card__heading" itemprop="name headline"> <a class="c-article-recommendations-card__link" itemprop="url" href="https://link.springer.com/10.1007/978-1-4939-1261-2_3?fromPaywallRec=true" data-track="select_recommendations_3" data-track-context="inline recommendations" data-track-action="click recommendations inline - 3" data-track-label="10.1007/978-1-4939-1261-2_3">Zebrafish Embryos as a Model to Study Bacterial Virulence </a> </h3> <div class="c-article-meta-recommendations" data-test="recommendation-info"> <span class="c-article-meta-recommendations__item-type">Chapter</span> <span class="c-article-meta-recommendations__date">© 2014</span> </div> </div> </article> </div> </div> </section> <script> window.dataLayer = window.dataLayer || []; window.dataLayer.push({ recommendations: { recommender: 'semantic', model: 'specter', policy_id: 'NA', timestamp: 1732372189, embedded_user: 'null' } }); </script> <div id="MagazineFulltextChapterBodySuffix"><section aria-labelledby="Bib1" data-title="References"><div class="c-article-section" id="Bib1-section"><h2 id="Bib1" class="c-article-section__title js-section-title js-c-reading-companion-sections-item"><span class="c-article-section__title-number"> </span>References</h2><div class="c-article-section__content" id="Bib1-content"><div data-container-section="references"><ol class="c-article-references" data-track-component="outbound reference" data-track-context="references section"><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="1."><p class="c-article-references__text" id="ref-CR1">Streisinger G, Walker C, Dower N et al (1981) Production of clones of homozygous diploid zebra fish (<i>Brachydanio rerio</i>). Nature 291:293–296</p><p class="c-article-references__links u-hide-print" id="ref-CR1-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/291293a0" data-track-item_id="10.1038/291293a0" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1038%2F291293a0" aria-label="Article reference 1" data-doi="10.1038/291293a0">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ASTN%3A280%3ADyaL3M7pslKmuw%253D%253D" aria-label="CAS reference 1">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 1" href="https://scholar.google.com/scholar_lookup?&title=Production%20of%20clones%20of%20homozygous%20diploid%20zebra%20fish%20%28Brachydanio%20rerio%29&journal=Nature&volume=291&pages=293-296&publication_year=1981&author=Streisinger%2CG&author=Walker%2CC&author=Dower%2CN"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="2."><p class="c-article-references__text" id="ref-CR2">Concordet JP, Ingham P (1994) Catch of the decade. Nature 369:19–20. Developmental biology</p><p class="c-article-references__links u-hide-print" id="ref-CR2-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/369019a0" data-track-item_id="10.1038/369019a0" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1038%2F369019a0" aria-label="Article reference 2" data-doi="10.1038/369019a0">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ASTN%3A280%3ADyaK2c3hslyjsA%253D%253D" aria-label="CAS reference 2">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 2" href="https://scholar.google.com/scholar_lookup?&title=Catch%20of%20the%20decade&journal=Nature&volume=369&pages=19-20&publication_year=1994&author=Concordet%2CJP&author=Ingham%2CP"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="3."><p class="c-article-references__text" id="ref-CR3">Kahn P (1994) Zebrafish hit the big time. Science 264:904–905</p><p class="c-article-references__links u-hide-print" id="ref-CR3-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1126/science.8178149" data-track-item_id="10.1126/science.8178149" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1126%2Fscience.8178149" aria-label="Article reference 3" data-doi="10.1126/science.8178149">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ASTN%3A280%3ADyaK2c3jtleksQ%253D%253D" aria-label="CAS reference 3">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 3" href="https://scholar.google.com/scholar_lookup?&title=Zebrafish%20hit%20the%20big%20time&journal=Science&volume=264&pages=904-905&publication_year=1994&author=Kahn%2CP"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="4."><p class="c-article-references__text" id="ref-CR4">Butler D (2000) Wellcome Trust funds bid to unravel zebrafish genome. Nature 408:503</p><p class="c-article-references__links u-hide-print" id="ref-CR4-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/35046231" data-track-item_id="10.1038/35046231" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1038%2F35046231" aria-label="Article reference 4" data-doi="10.1038/35046231">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BD3cXovFWkt7Y%253D" aria-label="CAS reference 4">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 4" href="https://scholar.google.com/scholar_lookup?&title=Wellcome%20Trust%20funds%20bid%20to%20unravel%20zebrafish%20genome&journal=Nature&volume=408&publication_year=2000&author=Butler%2CD"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="5."><p class="c-article-references__text" id="ref-CR5">Grunwald DJ, Eisen JS (2002) Headwaters of the zebrafish – emergence of a new model vertebrate. Nat Rev Genet 3:717–724</p><p class="c-article-references__links u-hide-print" id="ref-CR5-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/nrg892" data-track-item_id="10.1038/nrg892" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1038%2Fnrg892" aria-label="Article reference 5" data-doi="10.1038/nrg892">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BD38XmslagtLo%253D" aria-label="CAS reference 5">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 5" href="https://scholar.google.com/scholar_lookup?&title=Headwaters%20of%20the%20zebrafish%20%E2%80%93%20emergence%20of%20a%20new%20model%20vertebrate&journal=Nat%20Rev%20Genet&volume=3&pages=717-724&publication_year=2002&author=Grunwald%2CDJ&author=Eisen%2CJS"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="6."><p class="c-article-references__text" id="ref-CR6">Howe K, Clark MD, Torroja CF et al (2013) The zebrafish reference genome sequence and its relationship to the human genome. Nature 496:498–503</p><p class="c-article-references__links u-hide-print" id="ref-CR6-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/nature12111" data-track-item_id="10.1038/nature12111" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1038%2Fnature12111" aria-label="Article reference 6" data-doi="10.1038/nature12111">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BC3sXlvF2isb0%253D" aria-label="CAS reference 6">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 6" href="https://scholar.google.com/scholar_lookup?&title=The%20zebrafish%20reference%20genome%20sequence%20and%20its%20relationship%20to%20the%20human%20genome&journal=Nature&volume=496&pages=498-503&publication_year=2013&author=Howe%2CK&author=Clark%2CMD&author=Torroja%2CCF"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="7."><p class="c-article-references__text" id="ref-CR7">Hwang WY, Fu Y, Reyon D et al (2013) Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol 31:227–229</p><p class="c-article-references__links u-hide-print" id="ref-CR7-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/nbt.2501" data-track-item_id="10.1038/nbt.2501" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1038%2Fnbt.2501" aria-label="Article reference 7" data-doi="10.1038/nbt.2501">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BC3sXhsFCkurk%253D" aria-label="CAS reference 7">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 7" href="https://scholar.google.com/scholar_lookup?&title=Efficient%20genome%20editing%20in%20zebrafish%20using%20a%20CRISPR-Cas%20system&journal=Nat%20Biotechnol&volume=31&pages=227-229&publication_year=2013&author=Hwang%2CWY&author=Fu%2CY&author=Reyon%2CD"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="8."><p class="c-article-references__text" id="ref-CR8">Suster ML, Kikuta H, Urasaki A et al (2009) Transgenesis in zebrafish with the tol2 transposon system. Methods Mol Biol 561:41–63</p><p class="c-article-references__links u-hide-print" id="ref-CR8-links"><a data-track="click_references" rel="noopener" data-track-label="10.1007/978-1-60327-019-9_3" data-track-item_id="10.1007/978-1-60327-019-9_3" data-track-action="Article reference" data-track-value="Article reference" href="https://link.springer.com/doi/10.1007/978-1-60327-019-9_3" aria-label="Article reference 8" data-doi="10.1007/978-1-60327-019-9_3">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BC3cXhvVKgtb4%253D" aria-label="CAS reference 8">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 8" href="https://scholar.google.com/scholar_lookup?&title=Transgenesis%20in%20zebrafish%20with%20the%20tol2%20transposon%20system&journal=Methods%20Mol%20Biol&volume=561&pages=41-63&publication_year=2009&author=Suster%2CML&author=Kikuta%2CH&author=Urasaki%2CA"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="9."><p class="c-article-references__text" id="ref-CR9">Figueroa RJ, Carrasco-Avino G, Wichmann IA et al (2017) Reprimo tissue-specific expression pattern is conserved between zebrafish and human. PLoS One 12:e0178274</p><p class="c-article-references__links u-hide-print" id="ref-CR9-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1371/journal.pone.0178274" data-track-item_id="10.1371/journal.pone.0178274" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1371%2Fjournal.pone.0178274" aria-label="Article reference 9" data-doi="10.1371/journal.pone.0178274">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 9" href="https://scholar.google.com/scholar_lookup?&title=Reprimo%20tissue-specific%20expression%20pattern%20is%20conserved%20between%20zebrafish%20and%20human&journal=PLoS%20One&volume=12&publication_year=2017&author=Figueroa%2CRJ&author=Carrasco-Avino%2CG&author=Wichmann%2CIA"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="10."><p class="c-article-references__text" id="ref-CR10">Astin JW, Keerthisinghe P, Du L et al (2017) Innate immune cells and bacterial infection in zebrafish. Methods Cell Biol 138:31–60</p><p class="c-article-references__links u-hide-print" id="ref-CR10-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/bs.mcb.2016.08.002" data-track-item_id="10.1016/bs.mcb.2016.08.002" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1016%2Fbs.mcb.2016.08.002" aria-label="Article reference 10" data-doi="10.1016/bs.mcb.2016.08.002">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BB3cXhs1Gmu7vI" aria-label="CAS reference 10">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 10" href="https://scholar.google.com/scholar_lookup?&title=Innate%20immune%20cells%20and%20bacterial%20infection%20in%20zebrafish&journal=Methods%20Cell%20Biol&volume=138&pages=31-60&publication_year=2017&author=Astin%2CJW&author=Keerthisinghe%2CP&author=Du%2CL"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="11."><p class="c-article-references__text" id="ref-CR11">Novoa B, Figueras A (2012) Zebrafish: model for the study of inflammation and the innate immune response to infectious diseases. Adv Exp Med Biol 946:253–275</p><p class="c-article-references__links u-hide-print" id="ref-CR11-links"><a data-track="click_references" rel="noopener" data-track-label="10.1007/978-1-4614-0106-3_15" data-track-item_id="10.1007/978-1-4614-0106-3_15" data-track-action="Article reference" data-track-value="Article reference" href="https://link.springer.com/doi/10.1007/978-1-4614-0106-3_15" aria-label="Article reference 11" data-doi="10.1007/978-1-4614-0106-3_15">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BC38Xhs1GrtLjE" aria-label="CAS reference 11">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 11" href="https://scholar.google.com/scholar_lookup?&title=Zebrafish%3A%20model%20for%20the%20study%20of%20inflammation%20and%20the%20innate%20immune%20response%20to%20infectious%20diseases&journal=Adv%20Exp%20Med%20Biol&volume=946&pages=253-275&publication_year=2012&author=Novoa%2CB&author=Figueras%2CA"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="12."><p class="c-article-references__text" id="ref-CR12">Crane M, Hyatt A (2011) Viruses of fish: an overview of significant pathogens. Viruses 3:2025–2046</p><p class="c-article-references__links u-hide-print" id="ref-CR12-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.3390/v3112025" data-track-item_id="10.3390/v3112025" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.3390%2Fv3112025" aria-label="Article reference 12" data-doi="10.3390/v3112025">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 12" href="https://scholar.google.com/scholar_lookup?&title=Viruses%20of%20fish%3A%20an%20overview%20of%20significant%20pathogens&journal=Viruses&volume=3&pages=2025-2046&publication_year=2011&author=Crane%2CM&author=Hyatt%2CA"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="13."><p class="c-article-references__text" id="ref-CR13">Crim MJ, Riley LK (2012) Viral diseases in zebrafish: what is known and unknown. ILAR J 53:135–143</p><p class="c-article-references__links u-hide-print" id="ref-CR13-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1093/ilar.53.2.135" data-track-item_id="10.1093/ilar.53.2.135" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1093%2Filar.53.2.135" aria-label="Article reference 13" data-doi="10.1093/ilar.53.2.135">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BC3sXitFalurc%253D" aria-label="CAS reference 13">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 13" href="https://scholar.google.com/scholar_lookup?&title=Viral%20diseases%20in%20zebrafish%3A%20what%20is%20known%20and%20unknown&journal=ILAR%20J&volume=53&pages=135-143&publication_year=2012&author=Crim%2CMJ&author=Riley%2CLK"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="14."><p class="c-article-references__text" id="ref-CR14">Sanders GE, Batts WN, Winton JR (2003) Susceptibility of zebrafish <i>(Danio rerio</i>) to a model pathogen, spring viremia of carp virus. Comp Med 53:514–521</p><p class="c-article-references__links u-hide-print" id="ref-CR14-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BD3sXptFOjt7g%253D" aria-label="CAS reference 14">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 14" href="https://scholar.google.com/scholar_lookup?&title=Susceptibility%20of%20zebrafish%20%28Danio%20rerio%29%20to%20a%20model%20pathogen%2C%20spring%20viremia%20of%20carp%20virus&journal=Comp%20Med&volume=53&pages=514-521&publication_year=2003&author=Sanders%2CGE&author=Batts%2CWN&author=Winton%2CJR"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="15."><p class="c-article-references__text" id="ref-CR15">Levraud JP, Boudinot P, Colin I et al (2007) Identification of the zebrafish IFN receptor: implications for the origin of the vertebrate IFN system. J Immunol 178:4385–4394</p><p class="c-article-references__links u-hide-print" id="ref-CR15-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.4049/jimmunol.178.7.4385" data-track-item_id="10.4049/jimmunol.178.7.4385" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.4049%2Fjimmunol.178.7.4385" aria-label="Article reference 15" data-doi="10.4049/jimmunol.178.7.4385">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BD2sXjt1Wlu7Y%253D" aria-label="CAS reference 15">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 15" href="https://scholar.google.com/scholar_lookup?&title=Identification%20of%20the%20zebrafish%20IFN%20receptor%3A%20implications%20for%20the%20origin%20of%20the%20vertebrate%20IFN%20system&journal=J%20Immunol&volume=178&pages=4385-4394&publication_year=2007&author=Levraud%2CJP&author=Boudinot%2CP&author=Colin%2CI"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="16."><p class="c-article-references__text" id="ref-CR16">Aggad D, Mazel M, Boudinot P et al (2009) The two groups of zebrafish virus-induced interferons signal via distinct receptors with specific and shared chains. J Immunol 183:3924–3931</p><p class="c-article-references__links u-hide-print" id="ref-CR16-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.4049/jimmunol.0901495" data-track-item_id="10.4049/jimmunol.0901495" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.4049%2Fjimmunol.0901495" aria-label="Article reference 16" data-doi="10.4049/jimmunol.0901495">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BD1MXhtVOmu7zN" aria-label="CAS reference 16">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 16" href="https://scholar.google.com/scholar_lookup?&title=The%20two%20groups%20of%20zebrafish%20virus-induced%20interferons%20signal%20via%20distinct%20receptors%20with%20specific%20and%20shared%20chains&journal=J%20Immunol&volume=183&pages=3924-3931&publication_year=2009&author=Aggad%2CD&author=Mazel%2CM&author=Boudinot%2CP"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="17."><p class="c-article-references__text" id="ref-CR17">Feng H, Zhang YB, Zhang QM et al (2015) Zebrafish IRF1 regulates IFN antiviral response through binding to IFNϕ1 and IFNϕ3 promoters downstream of MyD88 signaling. J Immunol 194:1225–1238</p><p class="c-article-references__links u-hide-print" id="ref-CR17-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.4049/jimmunol.1402415" data-track-item_id="10.4049/jimmunol.1402415" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.4049%2Fjimmunol.1402415" aria-label="Article reference 17" data-doi="10.4049/jimmunol.1402415">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BC2MXhtVCgsb0%253D" aria-label="CAS reference 17">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 17" href="https://scholar.google.com/scholar_lookup?&title=Zebrafish%20IRF1%20regulates%20IFN%20antiviral%20response%20through%20binding%20to%20IFN%CF%951%20and%20IFN%CF%953%20promoters%20downstream%20of%20MyD88%20signaling&journal=J%20Immunol&volume=194&pages=1225-1238&publication_year=2015&author=Feng%2CH&author=Zhang%2CYB&author=Zhang%2CQM"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="18."><p class="c-article-references__text" id="ref-CR18">Varela M, Forn-Cuní G, Dios S et al (2016) Proinflammatory caspase a activation and an antiviral state are induced by a zebrafish perforin after possible cellular and functional diversification from a myeloid ancestor. J Innate Immun 8:43–56</p><p class="c-article-references__links u-hide-print" id="ref-CR18-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1159/000431287" data-track-item_id="10.1159/000431287" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1159%2F000431287" aria-label="Article reference 18" data-doi="10.1159/000431287">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BC28XhvV2ls7o%253D" aria-label="CAS reference 18">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 18" href="https://scholar.google.com/scholar_lookup?&title=Proinflammatory%20caspase%20a%20activation%20and%20an%20antiviral%20state%20are%20induced%20by%20a%20zebrafish%20perforin%20after%20possible%20cellular%20and%20functional%20diversification%20from%20a%20myeloid%20ancestor&journal=J%20Innate%20Immun&volume=8&pages=43-56&publication_year=2016&author=Varela%2CM&author=Forn-Cun%C3%AD%2CG&author=Dios%2CS"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="19."><p class="c-article-references__text" id="ref-CR19">Wu XM, Zhang J, Li PW et al (2020) NOD1 promotes antiviral signaling by binding viral RNA and regulating the interaction of MDA5 and MAVS. J Immunol 204:2216–2231</p><p class="c-article-references__links u-hide-print" id="ref-CR19-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.4049/jimmunol.1900667" data-track-item_id="10.4049/jimmunol.1900667" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.4049%2Fjimmunol.1900667" aria-label="Article reference 19" data-doi="10.4049/jimmunol.1900667">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BB3cXhtVGru7zE" aria-label="CAS reference 19">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 19" href="https://scholar.google.com/scholar_lookup?&title=NOD1%20promotes%20antiviral%20signaling%20by%20binding%20viral%20RNA%20and%20regulating%20the%20interaction%20of%20MDA5%20and%20MAVS&journal=J%20Immunol&volume=204&pages=2216-2231&publication_year=2020&author=Wu%2CXM&author=Zhang%2CJ&author=Li%2CPW"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="20."><p class="c-article-references__text" id="ref-CR20">Phelan PE, Mellon MT, Kim CH (2005) Functional characterization of full-length TLR3, IRAK-4, and TRAF6 in zebrafish (<i>Danio rerio</i>). Mol Immunol 42:1057–1071</p><p class="c-article-references__links u-hide-print" id="ref-CR20-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/j.molimm.2004.11.005" data-track-item_id="10.1016/j.molimm.2004.11.005" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1016%2Fj.molimm.2004.11.005" aria-label="Article reference 20" data-doi="10.1016/j.molimm.2004.11.005">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BD2MXjtFOmt7c%253D" aria-label="CAS reference 20">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 20" href="https://scholar.google.com/scholar_lookup?&title=Functional%20characterization%20of%20full-length%20TLR3%2C%20IRAK-4%2C%20and%20TRAF6%20in%20zebrafish%20%28Danio%20rerio%29&journal=Mol%20Immunol&volume=42&pages=1057-1071&publication_year=2005&author=Phelan%2CPE&author=Mellon%2CMT&author=Kim%2CCH"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="21."><p class="c-article-references__text" id="ref-CR21">Gabor KA, Charette JR, Pietraszewski MJ et al (2015) A DN-mda5 transgenic zebrafish model demonstrates that Mda5 plays an important role in snakehead rhabdovirus resistance. Dev Comp Immunol 51:298–304</p><p class="c-article-references__links u-hide-print" id="ref-CR21-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/j.dci.2015.01.006" data-track-item_id="10.1016/j.dci.2015.01.006" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1016%2Fj.dci.2015.01.006" aria-label="Article reference 21" data-doi="10.1016/j.dci.2015.01.006">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BC2MXhvVOkt70%253D" aria-label="CAS reference 21">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 21" href="https://scholar.google.com/scholar_lookup?&title=A%20DN-mda5%20transgenic%20zebrafish%20model%20demonstrates%20that%20Mda5%20plays%20an%20important%20role%20in%20snakehead%20rhabdovirus%20resistance&journal=Dev%20Comp%20Immunol&volume=51&pages=298-304&publication_year=2015&author=Gabor%2CKA&author=Charette%2CJR&author=Pietraszewski%2CMJ"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="22."><p class="c-article-references__text" id="ref-CR22">Liu X, Collodi P (2002) Novel form of fibronectin from zebrafish mediates infectious hematopoietic necrosis virus infection. J Virol 76:492–498</p><p class="c-article-references__links u-hide-print" id="ref-CR22-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1128/JVI.76.2.492-498.2002" data-track-item_id="10.1128/JVI.76.2.492-498.2002" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1128%2FJVI.76.2.492-498.2002" aria-label="Article reference 22" data-doi="10.1128/JVI.76.2.492-498.2002">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BD38Xht1Wktw%253D%253D" aria-label="CAS reference 22">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 22" href="https://scholar.google.com/scholar_lookup?&title=Novel%20form%20of%20fibronectin%20from%20zebrafish%20mediates%20infectious%20hematopoietic%20necrosis%20virus%20infection&journal=J%20Virol&volume=76&pages=492-498&publication_year=2002&author=Liu%2CX&author=Collodi%2CP"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="23."><p class="c-article-references__text" id="ref-CR23">Ludwig M, Palha N, Torhy C et al (2011) Whole-body analysis of a viral infection: vascular endothelium is a primary target of infectious hematopoietic necrosis virus in zebrafish larvae. PLoS Pathog 7:e1001269</p><p class="c-article-references__links u-hide-print" id="ref-CR23-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1371/journal.ppat.1001269" data-track-item_id="10.1371/journal.ppat.1001269" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1371%2Fjournal.ppat.1001269" aria-label="Article reference 23" data-doi="10.1371/journal.ppat.1001269">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BC3MXitVGgsLo%253D" aria-label="CAS reference 23">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 23" href="https://scholar.google.com/scholar_lookup?&title=Whole-body%20analysis%20of%20a%20viral%20infection%3A%20vascular%20endothelium%20is%20a%20primary%20target%20of%20infectious%20hematopoietic%20necrosis%20virus%20in%20zebrafish%20larvae&journal=PLoS%20Pathog&volume=7&publication_year=2011&author=Ludwig%2CM&author=Palha%2CN&author=Torhy%2CC"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="24."><p class="c-article-references__text" id="ref-CR24">Ghosh A, Banerjee A, Amzel LM et al (2019) Structure of the zebrafish galectin-1-L2 and model of its interaction with the infectious hematopoietic necrosis virus (IHNV) envelope glycoprotein. Glycobiology 29:419–430</p><p class="c-article-references__links u-hide-print" id="ref-CR24-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1093/glycob/cwz015" data-track-item_id="10.1093/glycob/cwz015" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1093%2Fglycob%2Fcwz015" aria-label="Article reference 24" data-doi="10.1093/glycob/cwz015">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BB3cXhtFWksbg%253D" aria-label="CAS reference 24">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 24" href="https://scholar.google.com/scholar_lookup?&title=Structure%20of%20the%20zebrafish%20galectin-1-L2%20and%20model%20of%20its%20interaction%20with%20the%20infectious%20hematopoietic%20necrosis%20virus%20%28IHNV%29%20envelope%20glycoprotein&journal=Glycobiology&volume=29&pages=419-430&publication_year=2019&author=Ghosh%2CA&author=Banerjee%2CA&author=Amzel%2CLM"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="25."><p class="c-article-references__text" id="ref-CR25">Novoa B, Romero A, Mulero V et al (2006) Zebrafish (<i>Danio rerio</i>) as a model for the study of vaccination against viral haemorrhagic septicemia virus (VHSV). Vaccine 24:5806–5816</p><p class="c-article-references__links u-hide-print" id="ref-CR25-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/j.vaccine.2006.05.015" data-track-item_id="10.1016/j.vaccine.2006.05.015" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1016%2Fj.vaccine.2006.05.015" aria-label="Article reference 25" data-doi="10.1016/j.vaccine.2006.05.015">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BD28Xms1Grs74%253D" aria-label="CAS reference 25">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 25" href="https://scholar.google.com/scholar_lookup?&title=Zebrafish%20%28Danio%20rerio%29%20as%20a%20model%20for%20the%20study%20of%20vaccination%20against%20viral%20haemorrhagic%20septicemia%20virus%20%28VHSV%29&journal=Vaccine&volume=24&pages=5806-5816&publication_year=2006&author=Novoa%2CB&author=Romero%2CA&author=Mulero%2CV"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="26."><p class="c-article-references__text" id="ref-CR26">Shanaka KASN, Jung S, Madushani KP et al (2022) Generation of viperin-knockout zebrafish by CRISPR/Cas9-mediated genome engineering and the effect of this mutation under VHSV infection. Fish Shellfish Immunol 131:672–681</p><p class="c-article-references__links u-hide-print" id="ref-CR26-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/j.fsi.2022.10.040" data-track-item_id="10.1016/j.fsi.2022.10.040" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1016%2Fj.fsi.2022.10.040" aria-label="Article reference 26" data-doi="10.1016/j.fsi.2022.10.040">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BB38XivVWrtrrN" aria-label="CAS reference 26">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 26" href="https://scholar.google.com/scholar_lookup?&title=Generation%20of%20viperin-knockout%20zebrafish%20by%20CRISPR%2FCas9-mediated%20genome%20engineering%20and%20the%20effect%20of%20this%20mutation%20under%20VHSV%20infection&journal=Fish%20Shellfish%20Immunol&volume=131&pages=672-681&publication_year=2022&author=Shanaka%2CKASN&author=Jung%2CS&author=Madushani%2CKP"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="27."><p class="c-article-references__text" id="ref-CR27">Lu MW, Chao YM, Guo TC et al (2008) The interferon response is involved in nervous necrosis virus acute and persistent infection in zebrafish infection model. Mol Immunol 45:1146–1152</p><p class="c-article-references__links u-hide-print" id="ref-CR27-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/j.molimm.2007.07.018" data-track-item_id="10.1016/j.molimm.2007.07.018" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1016%2Fj.molimm.2007.07.018" aria-label="Article reference 27" data-doi="10.1016/j.molimm.2007.07.018">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BD2sXht1GhsrfM" aria-label="CAS reference 27">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 27" href="https://scholar.google.com/scholar_lookup?&title=The%20interferon%20response%20is%20involved%20in%20nervous%20necrosis%20virus%20acute%20and%20persistent%20infection%20in%20zebrafish%20infection%20model&journal=Mol%20Immunol&volume=45&pages=1146-1152&publication_year=2008&author=Lu%2CMW&author=Chao%2CYM&author=Guo%2CTC"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="28."><p class="c-article-references__text" id="ref-CR28">Lama R, Pereiro P, Figueras A et al (2022) Zebrafish as a vertebrate model for studying nodavirus infections. Front Immunol 13:863096</p><p class="c-article-references__links u-hide-print" id="ref-CR28-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.3389/fimmu.2022.863096" data-track-item_id="10.3389/fimmu.2022.863096" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.3389%2Ffimmu.2022.863096" aria-label="Article reference 28" data-doi="10.3389/fimmu.2022.863096">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BB38XhtFOktbzN" aria-label="CAS reference 28">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 28" href="https://scholar.google.com/scholar_lookup?&title=Zebrafish%20as%20a%20vertebrate%20model%20for%20studying%20nodavirus%20infections&journal=Front%20Immunol&volume=13&publication_year=2022&author=Lama%2CR&author=Pereiro%2CP&author=Figueras%2CA"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="29."><p class="c-article-references__text" id="ref-CR29">Xu X, Zhang L, Weng S et al (2008) A zebrafish (<i>Danio rerio</i>) model of infectious spleen and kidney necrosis virus (ISKNV) infection. Virology 376:1–12</p><p class="c-article-references__links u-hide-print" id="ref-CR29-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/j.virol.2007.12.026" data-track-item_id="10.1016/j.virol.2007.12.026" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1016%2Fj.virol.2007.12.026" aria-label="Article reference 29" data-doi="10.1016/j.virol.2007.12.026">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BD1cXmsVegsb8%253D" aria-label="CAS reference 29">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 29" href="https://scholar.google.com/scholar_lookup?&title=A%20zebrafish%20%28Danio%20rerio%29%20model%20of%20infectious%20spleen%20and%20kidney%20necrosis%20virus%20%28ISKNV%29%20infection&journal=Virology&volume=376&pages=1-12&publication_year=2008&author=Xu%2CX&author=Zhang%2CL&author=Weng%2CS"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="30."><p class="c-article-references__text" id="ref-CR30">Qi L, Xiang Z (2015) Molecular cloning and expression analysis of an apoptosis-associated gene Daxx from zebrafish, <i>Danio rerio</i>. Fish Shellfish Immunol 45:59–66</p><p class="c-article-references__links u-hide-print" id="ref-CR30-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/j.fsi.2015.03.040" data-track-item_id="10.1016/j.fsi.2015.03.040" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1016%2Fj.fsi.2015.03.040" aria-label="Article reference 30" data-doi="10.1016/j.fsi.2015.03.040">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BC2MXntVCiurs%253D" aria-label="CAS reference 30">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 30" href="https://scholar.google.com/scholar_lookup?&title=Molecular%20cloning%20and%20expression%20analysis%20of%20an%20apoptosis-associated%20gene%20Daxx%20from%20zebrafish%2C%20Danio%20rerio&journal=Fish%20Shellfish%20Immunol&volume=45&pages=59-66&publication_year=2015&author=Qi%2CL&author=Xiang%2CZ"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="31."><p class="c-article-references__text" id="ref-CR31">Martín V, Mavian C, López Bueno A et al (2015) Establishment of a zebrafish infection model for the study of wild-type and recombinant European sheatfish virus. J Virol 89:10702–10706</p><p class="c-article-references__links u-hide-print" id="ref-CR31-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1128/JVI.01580-15" data-track-item_id="10.1128/JVI.01580-15" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1128%2FJVI.01580-15" aria-label="Article reference 31" data-doi="10.1128/JVI.01580-15">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 31" href="https://scholar.google.com/scholar_lookup?&title=Establishment%20of%20a%20zebrafish%20infection%20model%20for%20the%20study%20of%20wild-type%20and%20recombinant%20European%20sheatfish%20virus&journal=J%20Virol&volume=89&pages=10702-10706&publication_year=2015&author=Mart%C3%ADn%2CV&author=Mavian%2CC&author=L%C3%B3pez%20Bueno%2CA"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="32."><p class="c-article-references__text" id="ref-CR32">Burgos JS, Ripoll-Gomez J, Alfaro JM et al (2008) Zebrafish as a new model for herpes simplex virus type 1 infection. Zebrafish 5:323–333</p><p class="c-article-references__links u-hide-print" id="ref-CR32-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1089/zeb.2008.0552" data-track-item_id="10.1089/zeb.2008.0552" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1089%2Fzeb.2008.0552" aria-label="Article reference 32" data-doi="10.1089/zeb.2008.0552">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BD1MXktlKqtA%253D%253D" aria-label="CAS reference 32">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 32" href="https://scholar.google.com/scholar_lookup?&title=Zebrafish%20as%20a%20new%20model%20for%20herpes%20simplex%20virus%20type%201%20infection&journal=Zebrafish&volume=5&pages=323-333&publication_year=2008&author=Burgos%2CJS&author=Ripoll-Gomez%2CJ&author=Alfaro%2CJM"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="33."><p class="c-article-references__text" id="ref-CR33">Ge R, Zhou Y, Peng R et al (2015) Conservation of the STING-mediated cytosolic DNA sensing pathway in zebrafish. J Virol 89:7696–7706</p><p class="c-article-references__links u-hide-print" id="ref-CR33-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1128/JVI.01049-15" data-track-item_id="10.1128/JVI.01049-15" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1128%2FJVI.01049-15" aria-label="Article reference 33" data-doi="10.1128/JVI.01049-15">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BC2MXhtFyku77K" aria-label="CAS reference 33">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 33" href="https://scholar.google.com/scholar_lookup?&title=Conservation%20of%20the%20STING-mediated%20cytosolic%20DNA%20sensing%20pathway%20in%20zebrafish&journal=J%20Virol&volume=89&pages=7696-7706&publication_year=2015&author=Ge%2CR&author=Zhou%2CY&author=Peng%2CR"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="34."><p class="c-article-references__text" id="ref-CR34">Palha N, Guivel-Benhassine F, Briolat V et al (2013) Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish. PLoS Pathog 9:e1003619</p><p class="c-article-references__links u-hide-print" id="ref-CR34-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1371/journal.ppat.1003619" data-track-item_id="10.1371/journal.ppat.1003619" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1371%2Fjournal.ppat.1003619" aria-label="Article reference 34" data-doi="10.1371/journal.ppat.1003619">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BC3sXhsFOnur3F" aria-label="CAS reference 34">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 34" href="https://scholar.google.com/scholar_lookup?&title=Real-time%20whole-body%20visualization%20of%20Chikungunya%20Virus%20infection%20and%20host%20interferon%20response%20in%20zebrafish&journal=PLoS%20Pathog&volume=9&publication_year=2013&author=Palha%2CN&author=Guivel-Benhassine%2CF&author=Briolat%2CV"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="35."><p class="c-article-references__text" id="ref-CR35">Ren Y, Li X, Tian Z et al (2021) Zebrafish as an animal model for the antiviral RNA interference pathway. J Gen Virol 102. <a href="https://doi.org/10.1099/jgv.0.001552" data-track="click" data-track-action="external reference" data-track-label="10.1099/jgv.0.001552">https://doi.org/10.1099/jgv.0.001552</a></p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="36."><p class="c-article-references__text" id="ref-CR36">Sullivan C, Jurcyzszak D, Goody MF et al (2017) Using zebrafish models of human influenza a virus infections to screen antiviral drugs and characterize host immune cell responses. J Vis Exp 119:55235</p><p class="c-article-references__links u-hide-print" id="ref-CR36-links"><a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 36" href="https://scholar.google.com/scholar_lookup?&title=Using%20zebrafish%20models%20of%20human%20influenza%20a%20virus%20infections%20to%20screen%20antiviral%20drugs%20and%20characterize%20host%20immune%20cell%20responses&journal=J%20Vis%20Exp&volume=119&publication_year=2017&author=Sullivan%2CC&author=Jurcyzszak%2CD&author=Goody%2CMF"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="37."><p class="c-article-references__text" id="ref-CR37">Soos BL, Ballinger A, Weinstein M et al (2024) Color-flu fluorescent reporter influenza a viruses allow for in vivo studies of innate immune function in zebrafish. Viruses 16:155</p><p class="c-article-references__links u-hide-print" id="ref-CR37-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.3390/v16010155" data-track-item_id="10.3390/v16010155" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.3390%2Fv16010155" aria-label="Article reference 37" data-doi="10.3390/v16010155">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BB2cXivVGhs7g%253D" aria-label="CAS reference 37">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 37" href="https://scholar.google.com/scholar_lookup?&title=Color-flu%20fluorescent%20reporter%20influenza%20a%20viruses%20allow%20for%20in%20vivo%20studies%20of%20innate%20immune%20function%20in%20zebrafish&journal=Viruses&volume=16&publication_year=2024&author=Soos%2CBL&author=Ballinger%2CA&author=Weinstein%2CM"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="38."><p class="c-article-references__text" id="ref-CR38">Ding CB, Zhao Y, Zhang JP et al (2015) A zebrafish model for subgenomic hepatitis C virus replication. Int J Mol Med 35:791–797</p><p class="c-article-references__links u-hide-print" id="ref-CR38-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.3892/ijmm.2015.2063" data-track-item_id="10.3892/ijmm.2015.2063" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.3892%2Fijmm.2015.2063" aria-label="Article reference 38" data-doi="10.3892/ijmm.2015.2063">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BC2MXmsVWgtbY%253D" aria-label="CAS reference 38">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 38" href="https://scholar.google.com/scholar_lookup?&title=A%20zebrafish%20model%20for%20subgenomic%20hepatitis%20C%20virus%20replication&journal=Int%20J%20Mol%20Med&volume=35&pages=791-797&publication_year=2015&author=Ding%2CCB&author=Zhao%2CY&author=Zhang%2CJP"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="39."><p class="c-article-references__text" id="ref-CR39">Van Dycke J, Ny A, Conceição-Neto N et al (2019) A robust human norovirus replication model in zebrafish larvae. PLoS Pathog 15:e1008009</p><p class="c-article-references__links u-hide-print" id="ref-CR39-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1371/journal.ppat.1008009" data-track-item_id="10.1371/journal.ppat.1008009" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1371%2Fjournal.ppat.1008009" aria-label="Article reference 39" data-doi="10.1371/journal.ppat.1008009">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 39" href="https://scholar.google.com/scholar_lookup?&title=A%20robust%20human%20norovirus%20replication%20model%20in%20zebrafish%20larvae&journal=PLoS%20Pathog&volume=15&publication_year=2019&author=Dycke%2CJ&author=Ny%2CA&author=Concei%C3%A7%C3%A3o-Neto%2CN"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="40."><p class="c-article-references__text" id="ref-CR40">Balkrishna A, Solleti SK, Verma S et al (2020) Validation of a novel zebrafish model of dengue virus (DENV-3) pathology using the pentaherbal medicine denguenil vati. Biomol Ther 10:971</p><p class="c-article-references__links u-hide-print" id="ref-CR40-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BB3cXhsFWrsL7F" aria-label="CAS reference 40">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 40" href="https://scholar.google.com/scholar_lookup?&title=Validation%20of%20a%20novel%20zebrafish%20model%20of%20dengue%20virus%20%28DENV-3%29%20pathology%20using%20the%20pentaherbal%20medicine%20denguenil%20vati&journal=Biomol%20Ther&volume=10&publication_year=2020&author=Balkrishna%2CA&author=Solleti%2CSK&author=Verma%2CS"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="41."><p class="c-article-references__text" id="ref-CR41">Maleski ALA, Rosa JGS, Bernardo JTG et al (2022) Recapitulation of retinal damage in zebrafish larvae infected with Zika virus. Cells 11:1457</p><p class="c-article-references__links u-hide-print" id="ref-CR41-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.3390/cells11091457" data-track-item_id="10.3390/cells11091457" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.3390%2Fcells11091457" aria-label="Article reference 41" data-doi="10.3390/cells11091457">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BB38XhtlGgtr%252FO" aria-label="CAS reference 41">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 41" href="https://scholar.google.com/scholar_lookup?&title=Recapitulation%20of%20retinal%20damage%20in%20zebrafish%20larvae%20infected%20with%20Zika%20virus&journal=Cells&volume=11&publication_year=2022&author=Maleski%2CALA&author=Rosa%2CJGS&author=Bernardo%2CJTG"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="42."><p class="c-article-references__text" id="ref-CR42">López-Muñoz A, Roca FJ, Meseguer J et al (2009) New insights into the evolution of IFNs: zebrafish group II IFNs induce a rapid and transient expression of IFN-dependent genes and display powerful antiviral activities. J Immunol 182:3440–3449</p><p class="c-article-references__links u-hide-print" id="ref-CR42-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.4049/jimmunol.0802528" data-track-item_id="10.4049/jimmunol.0802528" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.4049%2Fjimmunol.0802528" aria-label="Article reference 42" data-doi="10.4049/jimmunol.0802528">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 42" href="https://scholar.google.com/scholar_lookup?&title=New%20insights%20into%20the%20evolution%20of%20IFNs%3A%20zebrafish%20group%20II%20IFNs%20induce%20a%20rapid%20and%20transient%20expression%20of%20IFN-dependent%20genes%20and%20display%20powerful%20antiviral%20activities&journal=J%20Immunol&volume=182&pages=3440-3449&publication_year=2009&author=L%C3%B3pez-Mu%C3%B1oz%2CA&author=Roca%2CFJ&author=Meseguer%2CJ"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="43."><p class="c-article-references__text" id="ref-CR43">Zou PF, Chang MX, Li Y et al (2016) NOD2 in zebrafish functions in antibacterial and also antiviral responses via NF-κB, and also MDA5, RIG-I and MAVS. Fish Shellfish Immunol 55:173–185</p><p class="c-article-references__links u-hide-print" id="ref-CR43-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/j.fsi.2016.05.031" data-track-item_id="10.1016/j.fsi.2016.05.031" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1016%2Fj.fsi.2016.05.031" aria-label="Article reference 43" data-doi="10.1016/j.fsi.2016.05.031">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BC28XpvV2qsLg%253D" aria-label="CAS reference 43">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 43" href="https://scholar.google.com/scholar_lookup?&title=NOD2%20in%20zebrafish%20functions%20in%20antibacterial%20and%20also%20antiviral%20responses%20via%20NF-%CE%BAB%2C%20and%20also%20MDA5%2C%20RIG-I%20and%20MAVS&journal=Fish%20Shellfish%20Immunol&volume=55&pages=173-185&publication_year=2016&author=Zou%2CPF&author=Chang%2CMX&author=Li%2CY"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="44."><p class="c-article-references__text" id="ref-CR44">He S, Salas-Vidal E, Rueb S et al (2006) Genetic and transcriptome characterization of model zebrafish cell lines. Zebrafish 3:441–453</p><p class="c-article-references__links u-hide-print" id="ref-CR44-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1089/zeb.2006.3.441" data-track-item_id="10.1089/zeb.2006.3.441" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1089%2Fzeb.2006.3.441" aria-label="Article reference 44" data-doi="10.1089/zeb.2006.3.441">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BD2sXmsVSjsw%253D%253D" aria-label="CAS reference 44">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 44" href="https://scholar.google.com/scholar_lookup?&title=Genetic%20and%20transcriptome%20characterization%20of%20model%20zebrafish%20cell%20lines&journal=Zebrafish&volume=3&pages=441-453&publication_year=2006&author=He%2CS&author=Salas-Vidal%2CE&author=Rueb%2CS"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="45."><p class="c-article-references__text" id="ref-CR45">Chen W, Zhao J, Mu D et al (2021) Pyroptosis mediates neutrophil extracellular trap formation during bacterial infection in zebrafish. J Immunol 206:1913–1922</p><p class="c-article-references__links u-hide-print" id="ref-CR45-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.4049/jimmunol.2001335" data-track-item_id="10.4049/jimmunol.2001335" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.4049%2Fjimmunol.2001335" aria-label="Article reference 45" data-doi="10.4049/jimmunol.2001335">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BB3MXhtFSrt7zF" aria-label="CAS reference 45">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 45" href="https://scholar.google.com/scholar_lookup?&title=Pyroptosis%20mediates%20neutrophil%20extracellular%20trap%20formation%20during%20bacterial%20infection%20in%20zebrafish&journal=J%20Immunol&volume=206&pages=1913-1922&publication_year=2021&author=Chen%2CW&author=Zhao%2CJ&author=Mu%2CD"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="46."><p class="c-article-references__text" id="ref-CR46">Cao L, Fang H, Yan D et al (2022) CD44a functions as a regulator of p53 signaling, apoptosis and autophagy in the antibacterial immune response. Commun Biol 5:889</p><p class="c-article-references__links u-hide-print" id="ref-CR46-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/s42003-022-03856-1" data-track-item_id="10.1038/s42003-022-03856-1" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1038%2Fs42003-022-03856-1" aria-label="Article reference 46" data-doi="10.1038/s42003-022-03856-1">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BB38XisV2gsLvN" aria-label="CAS reference 46">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 46" href="https://scholar.google.com/scholar_lookup?&title=CD44a%20functions%20as%20a%20regulator%20of%20p53%20signaling%2C%20apoptosis%20and%20autophagy%20in%20the%20antibacterial%20immune%20response&journal=Commun%20Biol&volume=5&publication_year=2022&author=Cao%2CL&author=Fang%2CH&author=Yan%2CD"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="47."><p class="c-article-references__text" id="ref-CR47">Wu X, Xiong F, Fang H et al (2021) Crosstalks between NOD1 and histone H2A contribute to host defense against Streptococcus agalactiae infection in zebrafish. Antibiotics (Basel) 10:861</p><p class="c-article-references__links u-hide-print" id="ref-CR47-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.3390/antibiotics10070861" data-track-item_id="10.3390/antibiotics10070861" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.3390%2Fantibiotics10070861" aria-label="Article reference 47" data-doi="10.3390/antibiotics10070861">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BB3MXitFyhsLjL" aria-label="CAS reference 47">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 47" href="https://scholar.google.com/scholar_lookup?&title=Crosstalks%20between%20NOD1%20and%20histone%20H2A%20contribute%20to%20host%20defense%20against%20Streptococcus%20agalactiae%20infection%20in%20zebrafish&journal=Antibiotics%20%28Basel%29&volume=10&publication_year=2021&author=Wu%2CX&author=Xiong%2CF&author=Fang%2CH"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="48."><p class="c-article-references__text" id="ref-CR48">Li PW, Zhang J, Chang MX (2024) Structure, function and immune evasion strategies of aquareoviruses, with focus on grass carp reovirus. Rev Aquac 16:410–432</p><p class="c-article-references__links u-hide-print" id="ref-CR48-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1111/raq.12844" data-track-item_id="10.1111/raq.12844" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1111%2Fraq.12844" aria-label="Article reference 48" data-doi="10.1111/raq.12844">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 48" href="https://scholar.google.com/scholar_lookup?&title=Structure%2C%20function%20and%20immune%20evasion%20strategies%20of%20aquareoviruses%2C%20with%20focus%20on%20grass%20carp%20reovirus&journal=Rev%20Aquac&volume=16&pages=410-432&publication_year=2024&author=Li%2CPW&author=Zhang%2CJ&author=Chang%2CMX"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="49."><p class="c-article-references__text" id="ref-CR49">Medishetti R, Balamurugan K, Yadavalli K et al (2022) CRISPR-Cas9-induced gene knockout in zebrafish. STAR Protoc 3:101779</p><p class="c-article-references__links u-hide-print" id="ref-CR49-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/j.xpro.2022.101779" data-track-item_id="10.1016/j.xpro.2022.101779" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1016%2Fj.xpro.2022.101779" aria-label="Article reference 49" data-doi="10.1016/j.xpro.2022.101779">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BB38Xislyqs7nE" aria-label="CAS reference 49">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 49" href="https://scholar.google.com/scholar_lookup?&title=CRISPR-Cas9-induced%20gene%20knockout%20in%20zebrafish&journal=STAR%20Protoc&volume=3&publication_year=2022&author=Medishetti%2CR&author=Balamurugan%2CK&author=Yadavalli%2CK"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="50."><p class="c-article-references__text" id="ref-CR50">Zhu J, Liu X, Cai X et al (2020) Zebrafish prmt7 negatively regulates antiviral responses by suppressing the retinoic acid-inducible gene-I-like receptor signaling. FASEB J 34:988–1000</p><p class="c-article-references__links u-hide-print" id="ref-CR50-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1096/fj.201902219R" data-track-item_id="10.1096/fj.201902219R" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1096%2Ffj.201902219R" aria-label="Article reference 50" data-doi="10.1096/fj.201902219R">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BB3cXhvFehsLY%253D" aria-label="CAS reference 50">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 50" href="https://scholar.google.com/scholar_lookup?&title=Zebrafish%20prmt7%20negatively%20regulates%20antiviral%20responses%20by%20suppressing%20the%20retinoic%20acid-inducible%20gene-I-like%20receptor%20signaling&journal=FASEB%20J&volume=34&pages=988-1000&publication_year=2020&author=Zhu%2CJ&author=Liu%2CX&author=Cai%2CX"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="51."><p class="c-article-references__text" id="ref-CR51">Li L, Tan J, Miao Y et al (2015) ROS and autophagy: interactions and molecular regulatory mechanisms. Cell Mol Neurobiol 35:615–621</p><p class="c-article-references__links u-hide-print" id="ref-CR51-links"><a data-track="click_references" rel="noopener" data-track-label="10.1007/s10571-015-0166-x" data-track-item_id="10.1007/s10571-015-0166-x" data-track-action="Article reference" data-track-value="Article reference" href="https://link.springer.com/doi/10.1007/s10571-015-0166-x" aria-label="Article reference 51" data-doi="10.1007/s10571-015-0166-x">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 51" href="https://scholar.google.com/scholar_lookup?&title=ROS%20and%20autophagy%3A%20interactions%20and%20molecular%20regulatory%20mechanisms&journal=Cell%20Mol%20Neurobiol&volume=35&pages=615-621&publication_year=2015&author=Li%2CL&author=Tan%2CJ&author=Miao%2CY"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="52."><p class="c-article-references__text" id="ref-CR52">Li P, Chang M (2021) Roles of PRR-mediated signaling pathways in the regulation of oxidative stress and inflammatory diseases. Int J Mol Sci 2:7688</p><p class="c-article-references__links u-hide-print" id="ref-CR52-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.3390/ijms22147688" data-track-item_id="10.3390/ijms22147688" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.3390%2Fijms22147688" aria-label="Article reference 52" data-doi="10.3390/ijms22147688">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 52" href="https://scholar.google.com/scholar_lookup?&title=Roles%20of%20PRR-mediated%20signaling%20pathways%20in%20the%20regulation%20of%20oxidative%20stress%20and%20inflammatory%20diseases&journal=Int%20J%20Mol%20Sci&volume=2&publication_year=2021&author=Li%2CP&author=Chang%2CM"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="53."><p class="c-article-references__text" id="ref-CR53">Razaghi B, Steele SL, Prykhozhij SV et al (2018) hace1 Influences zebrafish cardiac development via ROS-dependent mechanisms. Dev Dyn 247:289–303</p><p class="c-article-references__links u-hide-print" id="ref-CR53-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1002/dvdy.24600" data-track-item_id="10.1002/dvdy.24600" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1002%2Fdvdy.24600" aria-label="Article reference 53" data-doi="10.1002/dvdy.24600">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BC1cXhtlKlsbY%253D" aria-label="CAS reference 53">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 53" href="https://scholar.google.com/scholar_lookup?&title=hace1%20Influences%20zebrafish%20cardiac%20development%20via%20ROS-dependent%20mechanisms&journal=Dev%20Dyn&volume=247&pages=289-303&publication_year=2018&author=Razaghi%2CB&author=Steele%2CSL&author=Prykhozhij%2CSV"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="54."><p class="c-article-references__text" id="ref-CR54">Ampomah PB, Lim LHK (2020) Influenza A virus-induced apoptosis and virus propagation. Apoptosis 25:1–11</p><p class="c-article-references__links u-hide-print" id="ref-CR54-links"><a data-track="click_references" rel="noopener" data-track-label="10.1007/s10495-019-01575-3" data-track-item_id="10.1007/s10495-019-01575-3" data-track-action="Article reference" data-track-value="Article reference" href="https://link.springer.com/doi/10.1007/s10495-019-01575-3" aria-label="Article reference 54" data-doi="10.1007/s10495-019-01575-3">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-action="CAS reference" data-track-value="CAS reference" href="/articles/cas-redirect/1%3ACAS%3A528%3ADC%252BC1MXitVKrtL3M" aria-label="CAS reference 54">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 54" href="https://scholar.google.com/scholar_lookup?&title=Influenza%20A%20virus-induced%20apoptosis%20and%20virus%20propagation&journal=Apoptosis&volume=25&pages=1-11&publication_year=2020&author=Ampomah%2CPB&author=Lim%2CLHK"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="55."><p class="c-article-references__text" id="ref-CR55">Kvansakul M (2017) Viral infection and apoptosis. Viruses 9:356</p><p class="c-article-references__links u-hide-print" id="ref-CR55-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.3390/v9120356" data-track-item_id="10.3390/v9120356" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.3390%2Fv9120356" aria-label="Article reference 55" data-doi="10.3390/v9120356">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 55" href="https://scholar.google.com/scholar_lookup?&title=Viral%20infection%20and%20apoptosis&journal=Viruses&volume=9&publication_year=2017&author=Kvansakul%2CM"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="56."><p class="c-article-references__text" id="ref-CR56">Tucker B, Lardelli M (2007) A rapid apoptosis assay measuring relative acridine orange fluorescence in zebrafish embryos. Zebrafish 4:113–116</p><p class="c-article-references__links u-hide-print" id="ref-CR56-links"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1089/zeb.2007.0508" data-track-item_id="10.1089/zeb.2007.0508" data-track-action="Article reference" data-track-value="Article reference" href="https://doi.org/10.1089%2Fzeb.2007.0508" aria-label="Article reference 56" data-doi="10.1089/zeb.2007.0508">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 56" href="https://scholar.google.com/scholar_lookup?&title=A%20rapid%20apoptosis%20assay%20measuring%20relative%20acridine%20orange%20fluorescence%20in%20zebrafish%20embryos&journal=Zebrafish&volume=4&pages=113-116&publication_year=2007&author=Tucker%2CB&author=Lardelli%2CM"> Google Scholar</a> </p></li></ol><p class="c-article-references__download u-hide-print"><a data-track="click" data-track-action="download citation references" data-track-label="link" rel="nofollow" href="https://citation-needed.springer.com/v2/references/10.1007/978-1-0716-4108-8_22?format=refman&flavour=references">Download references<svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-download-medium"></use></svg></a></p></div></div></div></section></div><section data-title="Acknowledgments" lang="en"><div class="c-article-section" id="Ack1-section"><h2 id="Ack1" class="c-article-section__title js-section-title js-c-reading-companion-sections-item"><span class="c-article-section__title-number"> </span>Acknowledgments</h2><div class="c-article-section__content" id="Ack1-content"><p>This work was supposed by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB0730100) and Joint Funds of the National Natural Science Foundation of China (Grant No. U22A20535).</p></div></div></section><section aria-labelledby="author-information" data-title="Author information"><div class="c-article-section" id="author-information-section"><h2 id="author-information" class="c-article-section__title js-section-title js-c-reading-companion-sections-item"><span class="c-article-section__title-number"> </span>Author information</h2><div class="c-article-section__content" id="author-information-content"><h3 class="c-article__sub-heading" id="affiliations">Authors and Affiliations</h3><ol class="c-article-author-affiliation__list"><li id="Aff3"><p class="c-article-author-affiliation__address">Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, China</p><p class="c-article-author-affiliation__authors-list">Xiao Man Wu, Si Yao Zheng & Ming Xian Chang</p></li><li id="Aff4"><p class="c-article-author-affiliation__address">College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China</p><p class="c-article-author-affiliation__authors-list">Si Yao Zheng & Ming Xian Chang</p></li></ol><div class="u-js-hide u-hide-print" data-test="author-info"><span class="c-article__sub-heading">Authors</span><ol class="c-article-authors-search u-list-reset"><li id="auth-Xiao_Man-Wu"><span class="c-article-authors-search__title u-h3 js-search-name">Xiao Man Wu</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?dc.creator=Xiao%20Man%20Wu" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Xiao%20Man%20Wu" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Xiao%20Man%20Wu%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Si_Yao-Zheng"><span class="c-article-authors-search__title u-h3 js-search-name">Si Yao Zheng</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?dc.creator=Si%20Yao%20Zheng" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Si%20Yao%20Zheng" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Si%20Yao%20Zheng%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Ming_Xian-Chang"><span class="c-article-authors-search__title u-h3 js-search-name">Ming Xian Chang</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?dc.creator=Ming%20Xian%20Chang" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Ming%20Xian%20Chang" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Ming%20Xian%20Chang%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li></ol></div><h3 class="c-article__sub-heading" id="corresponding-author">Corresponding author</h3><p id="corresponding-author-list">Correspondence to <a id="corresp-c1" href="mailto:mingxianchang@ihb.ac.cn">Ming Xian Chang </a>.</p></div></div></section><section aria-labelledby="editor-information" data-title="Editor information"><div class="c-article-section" id="editor-information-section"><h2 id="editor-information" class="c-article-section__title js-section-title js-c-reading-companion-sections-item"><span class="c-article-section__title-number"> </span>Editor information</h2><div class="c-article-section__content" id="editor-information-content"><h3 class="c-article__sub-heading" id="editor-affiliations">Editors and Affiliations</h3><ol class="c-article-author-affiliation__list"><li id="Aff2"><p class="c-article-author-affiliation__address">Department of Microbiology, Immunology, and Infection Diseases, University of Calgary, Calgary, AB, Canada</p><p class="c-article-author-affiliation__authors-list">Chunfu Zheng </p></li></ol></div></div></section><section data-title="Rights and permissions" lang="en"><div class="c-article-section" id="rightslink-section"><h2 id="rightslink" class="c-article-section__title js-section-title js-c-reading-companion-sections-item"><span class="c-article-section__title-number"> </span>Rights and permissions</h2><div class="c-article-section__content" id="rightslink-content"><p class="c-article-rights" data-test="rightslink-content"><a data-track="click" data-track-action="view rights and permissions" data-track-label="link" href="https://s100.copyright.com/AppDispatchServlet?publisherName=SpringerNature&orderBeanReset=true&orderSource=SpringerLink&title=Zebrafish%20as%20a%20Model%20for%20Investigating%20Antiviral%20Innate%20Immunity&author=Xiao%20Man%20Wu%2C%20Si%20Yao%20Zheng%2C%20Ming%20Xian%20Chang&contentID=10.1007%2F978-1-0716-4108-8_22&copyright=The%20Author%28s%29%2C%20under%20exclusive%20license%20to%20Springer%20Science%2BBusiness%20Media%2C%20LLC%2C%20part%20of%20Springer%20Nature&publication=eBook&publicationDate=2025&startPage=221&endPage=236&imprint=The%20Author%28s%29%2C%20under%20exclusive%20license%20to%20Springer%20Science%2BBusiness%20Media%2C%20LLC%2C%20part%20of%20Springer%20Nature">Reprints and permissions</a></p></div></div></section><section data-title="Copyright information"><div class="c-article-section" id="copyright-information-section"><h2 id="copyright-information" class="c-article-section__title js-section-title js-c-reading-companion-sections-item"><span class="c-article-section__title-number"> </span>Copyright information</h2><div class="c-article-section__content" id="copyright-information-content"><p>© 2025 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature</p></div></div></section><section aria-labelledby="chapter-info" data-title="About this protocol" lang="en"><div class="c-article-section" id="chapter-info-section"><h2 id="chapter-info" class="c-article-section__title js-section-title js-c-reading-companion-sections-item"><span class="c-article-section__title-number"> </span>About this protocol</h2><div class="c-article-section__content" id="chapter-info-content"><div class="c-bibliographic-information"><div class="u-hide-print c-bibliographic-information__column c-bibliographic-information__column--border"><a data-crossmark="10.1007/978-1-0716-4108-8_22" target="_blank" rel="noopener" href="https://crossmark.crossref.org/dialog/?doi=10.1007/978-1-0716-4108-8_22" data-track="click" data-track-action="Click Crossmark" data-track-label="link" data-test="crossmark"><img loading="lazy" width="57" height="81" alt="Check for updates. Verify currency and authenticity via CrossMark" src="data:image/svg+xml;base64,<svg height="81" width="57" xmlns="http://www.w3.org/2000/svg"><g fill="none" fill-rule="evenodd"><path d="m17.35 35.45 21.3-14.2v-17.03h-21.3" fill="#989898"/><path d="m38.65 35.45-21.3-14.2v-17.03h21.3" fill="#747474"/><path d="m28 .5c-12.98 0-23.5 10.52-23.5 23.5s10.52 23.5 23.5 23.5 23.5-10.52 23.5-23.5c0-6.23-2.48-12.21-6.88-16.62-4.41-4.4-10.39-6.88-16.62-6.88zm0 41.25c-9.8 0-17.75-7.95-17.75-17.75s7.95-17.75 17.75-17.75 17.75 7.95 17.75 17.75c0 4.71-1.87 9.22-5.2 12.55s-7.84 5.2-12.55 5.2z" fill="#535353"/><path d="m41 36c-5.81 6.23-15.23 7.45-22.43 2.9-7.21-4.55-10.16-13.57-7.03-21.5l-4.92-3.11c-4.95 10.7-1.19 23.42 8.78 29.71 9.97 6.3 23.07 4.22 30.6-4.86z" fill="#9c9c9c"/><path d="m.2 58.45c0-.75.11-1.42.33-2.01s.52-1.09.91-1.5c.38-.41.83-.73 1.34-.94.51-.22 1.06-.32 1.65-.32.56 0 1.06.11 1.51.35.44.23.81.5 1.1.81l-.91 1.01c-.24-.24-.49-.42-.75-.56-.27-.13-.58-.2-.93-.2-.39 0-.73.08-1.05.23-.31.16-.58.37-.81.66-.23.28-.41.63-.53 1.04-.13.41-.19.88-.19 1.39 0 1.04.23 1.86.68 2.46.45.59 1.06.88 1.84.88.41 0 .77-.07 1.07-.23s.59-.39.85-.68l.91 1c-.38.43-.8.76-1.28.99-.47.22-1 .34-1.58.34-.59 0-1.13-.1-1.64-.31-.5-.2-.94-.51-1.31-.91-.38-.4-.67-.9-.88-1.48-.22-.59-.33-1.26-.33-2.02zm8.4-5.33h1.61v2.54l-.05 1.33c.29-.27.61-.51.96-.72s.76-.31 1.24-.31c.73 0 1.27.23 1.61.71.33.47.5 1.14.5 2.02v4.31h-1.61v-4.1c0-.57-.08-.97-.25-1.21-.17-.23-.45-.35-.83-.35-.3 0-.56.08-.79.22-.23.15-.49.36-.78.64v4.8h-1.61zm7.37 6.45c0-.56.09-1.06.26-1.51.18-.45.42-.83.71-1.14.29-.3.63-.54 1.01-.71.39-.17.78-.25 1.18-.25.47 0 .88.08 1.23.24.36.16.65.38.89.67s.42.63.54 1.03c.12.41.18.84.18 1.32 0 .32-.02.57-.07.76h-4.36c.07.62.29 1.1.65 1.44.36.33.82.5 1.38.5.29 0 .57-.04.83-.13s.51-.21.76-.37l.55 1.01c-.33.21-.69.39-1.09.53-.41.14-.83.21-1.26.21-.48 0-.92-.08-1.34-.25-.41-.16-.76-.4-1.07-.7-.31-.31-.55-.69-.72-1.13-.18-.44-.26-.95-.26-1.52zm4.6-.62c0-.55-.11-.98-.34-1.28-.23-.31-.58-.47-1.06-.47-.41 0-.77.15-1.07.45-.31.29-.5.73-.58 1.3zm2.5.62c0-.57.09-1.08.28-1.53.18-.44.43-.82.75-1.13s.69-.54 1.1-.71c.42-.16.85-.24 1.31-.24.45 0 .84.08 1.17.23s.61.34.85.57l-.77 1.02c-.19-.16-.38-.28-.56-.37-.19-.09-.39-.14-.61-.14-.56 0-1.01.21-1.35.63-.35.41-.52.97-.52 1.67 0 .69.17 1.24.51 1.66.34.41.78.62 1.32.62.28 0 .54-.06.78-.17.24-.12.45-.26.64-.42l.67 1.03c-.33.29-.69.51-1.08.65-.39.15-.78.23-1.18.23-.46 0-.9-.08-1.31-.24-.4-.16-.75-.39-1.05-.7s-.53-.69-.7-1.13c-.17-.45-.25-.96-.25-1.53zm6.91-6.45h1.58v6.17h.05l2.54-3.16h1.77l-2.35 2.8 2.59 4.07h-1.75l-1.77-2.98-1.08 1.23v1.75h-1.58zm13.69 1.27c-.25-.11-.5-.17-.75-.17-.58 0-.87.39-.87 1.16v.75h1.34v1.27h-1.34v5.6h-1.61v-5.6h-.92v-1.2l.92-.07v-.72c0-.35.04-.68.13-.98.08-.31.21-.57.4-.79s.42-.39.71-.51c.28-.12.63-.18 1.04-.18.24 0 .48.02.69.07.22.05.41.1.57.17zm.48 5.18c0-.57.09-1.08.27-1.53.17-.44.41-.82.72-1.13.3-.31.65-.54 1.04-.71.39-.16.8-.24 1.23-.24s.84.08 1.24.24c.4.17.74.4 1.04.71s.54.69.72 1.13c.19.45.28.96.28 1.53s-.09 1.08-.28 1.53c-.18.44-.42.82-.72 1.13s-.64.54-1.04.7-.81.24-1.24.24-.84-.08-1.23-.24-.74-.39-1.04-.7c-.31-.31-.55-.69-.72-1.13-.18-.45-.27-.96-.27-1.53zm1.65 0c0 .69.14 1.24.43 1.66.28.41.68.62 1.18.62.51 0 .9-.21 1.19-.62.29-.42.44-.97.44-1.66 0-.7-.15-1.26-.44-1.67-.29-.42-.68-.63-1.19-.63-.5 0-.9.21-1.18.63-.29.41-.43.97-.43 1.67zm6.48-3.44h1.33l.12 1.21h.05c.24-.44.54-.79.88-1.02.35-.24.7-.36 1.07-.36.32 0 .59.05.78.14l-.28 1.4-.33-.09c-.11-.01-.23-.02-.38-.02-.27 0-.56.1-.86.31s-.55.58-.77 1.1v4.2h-1.61zm-47.87 15h1.61v4.1c0 .57.08.97.25 1.2.17.24.44.35.81.35.3 0 .57-.07.8-.22.22-.15.47-.39.73-.73v-4.7h1.61v6.87h-1.32l-.12-1.01h-.04c-.3.36-.63.64-.98.86-.35.21-.76.32-1.24.32-.73 0-1.27-.24-1.61-.71-.33-.47-.5-1.14-.5-2.02zm9.46 7.43v2.16h-1.61v-9.59h1.33l.12.72h.05c.29-.24.61-.45.97-.63.35-.17.72-.26 1.1-.26.43 0 .81.08 1.15.24.33.17.61.4.84.71.24.31.41.68.53 1.11.13.42.19.91.19 1.44 0 .59-.09 1.11-.25 1.57-.16.47-.38.85-.65 1.16-.27.32-.58.56-.94.73-.35.16-.72.25-1.1.25-.3 0-.6-.07-.9-.2s-.59-.31-.87-.56zm0-2.3c.26.22.5.37.73.45.24.09.46.13.66.13.46 0 .84-.2 1.15-.6.31-.39.46-.98.46-1.77 0-.69-.12-1.22-.35-1.61-.23-.38-.61-.57-1.13-.57-.49 0-.99.26-1.52.77zm5.87-1.69c0-.56.08-1.06.25-1.51.16-.45.37-.83.65-1.14.27-.3.58-.54.93-.71s.71-.25 1.08-.25c.39 0 .73.07 1 .2.27.14.54.32.81.55l-.06-1.1v-2.49h1.61v9.88h-1.33l-.11-.74h-.06c-.25.25-.54.46-.88.64-.33.18-.69.27-1.06.27-.87 0-1.56-.32-2.07-.95s-.76-1.51-.76-2.65zm1.67-.01c0 .74.13 1.31.4 1.7.26.38.65.58 1.15.58.51 0 .99-.26 1.44-.77v-3.21c-.24-.21-.48-.36-.7-.45-.23-.08-.46-.12-.7-.12-.45 0-.82.19-1.13.59-.31.39-.46.95-.46 1.68zm6.35 1.59c0-.73.32-1.3.97-1.71.64-.4 1.67-.68 3.08-.84 0-.17-.02-.34-.07-.51-.05-.16-.12-.3-.22-.43s-.22-.22-.38-.3c-.15-.06-.34-.1-.58-.1-.34 0-.68.07-1 .2s-.63.29-.93.47l-.59-1.08c.39-.24.81-.45 1.28-.63.47-.17.99-.26 1.54-.26.86 0 1.51.25 1.93.76s.63 1.25.63 2.21v4.07h-1.32l-.12-.76h-.05c-.3.27-.63.48-.98.66s-.73.27-1.14.27c-.61 0-1.1-.19-1.48-.56-.38-.36-.57-.85-.57-1.46zm1.57-.12c0 .3.09.53.27.67.19.14.42.21.71.21.28 0 .54-.07.77-.2s.48-.31.73-.56v-1.54c-.47.06-.86.13-1.18.23-.31.09-.57.19-.76.31s-.33.25-.41.4c-.09.15-.13.31-.13.48zm6.29-3.63h-.98v-1.2l1.06-.07.2-1.88h1.34v1.88h1.75v1.27h-1.75v3.28c0 .8.32 1.2.97 1.2.12 0 .24-.01.37-.04.12-.03.24-.07.34-.11l.28 1.19c-.19.06-.4.12-.64.17-.23.05-.49.08-.76.08-.4 0-.74-.06-1.02-.18-.27-.13-.49-.3-.67-.52-.17-.21-.3-.48-.37-.78-.08-.3-.12-.64-.12-1.01zm4.36 2.17c0-.56.09-1.06.27-1.51s.41-.83.71-1.14c.29-.3.63-.54 1.01-.71.39-.17.78-.25 1.18-.25.47 0 .88.08 1.23.24.36.16.65.38.89.67s.42.63.54 1.03c.12.41.18.84.18 1.32 0 .32-.02.57-.07.76h-4.37c.08.62.29 1.1.65 1.44.36.33.82.5 1.38.5.3 0 .58-.04.84-.13.25-.09.51-.21.76-.37l.54 1.01c-.32.21-.69.39-1.09.53s-.82.21-1.26.21c-.47 0-.92-.08-1.33-.25-.41-.16-.77-.4-1.08-.7-.3-.31-.54-.69-.72-1.13-.17-.44-.26-.95-.26-1.52zm4.61-.62c0-.55-.11-.98-.34-1.28-.23-.31-.58-.47-1.06-.47-.41 0-.77.15-1.08.45-.31.29-.5.73-.57 1.3zm3.01 2.23c.31.24.61.43.92.57.3.13.63.2.98.2.38 0 .65-.08.83-.23s.27-.35.27-.6c0-.14-.05-.26-.13-.37-.08-.1-.2-.2-.34-.28-.14-.09-.29-.16-.47-.23l-.53-.22c-.23-.09-.46-.18-.69-.3-.23-.11-.44-.24-.62-.4s-.33-.35-.45-.55c-.12-.21-.18-.46-.18-.75 0-.61.23-1.1.68-1.49.44-.38 1.06-.57 1.83-.57.48 0 .91.08 1.29.25s.71.36.99.57l-.74.98c-.24-.17-.49-.32-.73-.42-.25-.11-.51-.16-.78-.16-.35 0-.6.07-.76.21-.17.15-.25.33-.25.54 0 .14.04.26.12.36s.18.18.31.26c.14.07.29.14.46.21l.54.19c.23.09.47.18.7.29s.44.24.64.4c.19.16.34.35.46.58.11.23.17.5.17.82 0 .3-.06.58-.17.83-.12.26-.29.48-.51.68-.23.19-.51.34-.84.45-.34.11-.72.17-1.15.17-.48 0-.95-.09-1.41-.27-.46-.19-.86-.41-1.2-.68z" fill="#535353"/></g></svg>"></a></div><div class="c-bibliographic-information__column"><h3 class="c-article__sub-heading" id="citeas">Cite this protocol</h3><p class="c-bibliographic-information__citation" data-test="bibliographic-information__cite_this_chapter">Wu, X.M., Zheng, S.Y., Chang, M.X. (2025). Zebrafish as a Model for Investigating Antiviral Innate Immunity. In: Zheng, C. (eds) Antiviral Innate Immunity. Methods in Molecular Biology, vol 2854. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-4108-8_22</p><h3 class="c-bibliographic-information__download-citation u-mb-8 u-mt-16 u-hide-print">Download citation</h3><ul class="c-bibliographic-information__download-citation-list"><li class="c-bibliographic-information__download-citation-item"><a data-test="citation-link" data-track="click" data-track-action="download chapter citation" data-track-label="link" data-track-external="" title="Download this article's citation as a .RIS file" rel="nofollow" href="https://citation-needed.springer.com/v2/references/10.1007/978-1-0716-4108-8_22?format=refman&flavour=citation">.RIS<svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-download-medium"></use></svg></a></li><li class="c-bibliographic-information__download-citation-item"><a data-test="citation-link" data-track="click" data-track-action="download chapter citation" data-track-label="link" data-track-external="" title="Download this article's citation as a .ENW file" rel="nofollow" href="https://citation-needed.springer.com/v2/references/10.1007/978-1-0716-4108-8_22?format=endnote&flavour=citation">.ENW<svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-download-medium"></use></svg></a></li><li class="c-bibliographic-information__download-citation-item"><a data-test="citation-link" data-track="click" data-track-action="download chapter citation" data-track-label="link" data-track-external="" title="Download this article's citation as a .BIB file" rel="nofollow" href="https://citation-needed.springer.com/v2/references/10.1007/978-1-0716-4108-8_22?format=bibtex&flavour=citation">.BIB<svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-download-medium"></use></svg></a></li></ul><ul class="c-bibliographic-information__list u-mb-24" data-test="publication-history"><li class="c-bibliographic-information__list-item c-bibliographic-information__list-item--chapter-doi"><p data-test="bibliographic-information__doi"><abbr title="Digital Object Identifier">DOI</abbr><span class="u-hide">: </span><span class="c-bibliographic-information__value">https://doi.org/10.1007/978-1-0716-4108-8_22</span></p></li><li class="c-bibliographic-information__list-item"><p>Published<span class="u-hide">: </span><span class="c-bibliographic-information__value"><time datetime="2024-08-28">28 August 2024</time></span></p></li><li class="c-bibliographic-information__list-item"><p data-test="bibliographic-information__publisher-name"> Publisher Name<span class="u-hide">: </span><span class="c-bibliographic-information__value">Humana, New York, NY</span></p></li><li class="c-bibliographic-information__list-item"><p data-test="bibliographic-information__pisbn"> Print ISBN<span class="u-hide">: </span><span class="c-bibliographic-information__value">978-1-0716-4107-1</span></p></li><li class="c-bibliographic-information__list-item"><p data-test="bibliographic-information__eisbn"> Online ISBN<span class="u-hide">: </span><span class="c-bibliographic-information__value">978-1-0716-4108-8</span></p></li><li class="c-bibliographic-information__list-item"><p data-test="bibliographic-information__package">eBook Packages<span class="u-hide">: </span><span class="c-bibliographic-information__multi-value"><a href="/search?facet-content-type=%22Book%22&package=12345&facet-start-year=2025&facet-end-year=2025">Springer Protocols</a></span></p></li></ul><div data-component="chapter-info-list"></div></div></div></div></div></section><section aria-labelledby="publish-with-us" data-title="Publish with us" lang="en"><div class="c-article-section" id="publish-with-us-section"><h2 id="publish-with-us" class="c-article-section__title js-section-title js-c-reading-companion-sections-item"><span class="c-article-section__title-number"> </span>Publish with us</h2><div class="c-article-section__content" id="publish-with-us-content"><p><a class="app-article-policy-section-external-link" href="https://www.springernature.com/gp/policies/book-publishing-policies" data-track="click" data-track-action="publishing policies" data-track-label="link">Policies and ethics</a><svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon app-article-policy-section-external-link-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-external-link-small"></use></svg></p></div></div></section> </div> </article> </main> <div class="c-article-sidebar u-text-sm u-hide-print l-with-sidebar__sidebar" id="sidebar" data-container-type="reading-companion" data-track-component="reading companion"> <aside> <div data-test="editorial-summary"> </div> <div class="c-reading-companion"> <div class="c-reading-companion__sticky" data-component="reading-companion-sticky" data-test="reading-companion-sticky"> <div data-test="access-chapter-desktop" class="app-article-access"> <h2 class="app-article-access__heading">Access this chapter</h2> <div class="u-ma-16 u-clear-both"> <a href="//wayf.springernature.com?redirect_uri=https%3A%2F%2Flink.springer.com%2Fprotocol%2F10.1007%2F978-1-0716-4108-8_22%3Ferror%3Dcookies_not_supported%26code%3Db7045d23-fc9f-49e9-b549-6a41c1f97f27" class="u-button u-button--full-width u-button--primary u-justify-content-space-between c-pdf-download__link" data-track="click" data-track-action="institution access" data-track-label="button"> <span data-test="access-via-institution">Log in via an institution</span> <svg aria-hidden="true" focusable="false" width="16" height="16" class="u-icon"> <use xlink:href="#icon-eds-i-arrow-right-medium"></use> </svg> </a> </div> <div data-test="buy-box-desktop"> <div class="sprcom-buybox-darwin sprcom-buybox-darwin-b buybox" id="sprcom-buybox-darwin-b"> <div> <div class="c-springer-plus"> <h2 class="springer-plus-heading">Subscribe and save</h2> <div class="springer-plus"> <div class="springer-plus-headline"> <div class="springer-plus-title"> <svg aria-hidden="true" focusable="false" width="16" height="16" class="u-icon"> <use xlink:href="#icon-eds-i-check-filled-medium"></use> </svg><span>Springer+ Basic</span> </div> <div class="dd price-amount-springer-plus"> €32.70 /Month </div> </div> <ul class="buying-option-usps"> <li>Get 10 units per month</li> <li>Download Article/Chapter or eBook</li> <li>1 Unit = 1 Article or 1 Chapter</li> <li>Cancel anytime</li> </ul><a href="https://link.springer.com/product/springer-plus" class="u-button u-button--full-width u-button--secondary" id="btn-subscribe-springerPlus" data-track="click||click_springer_subscribe" data-track-context="buy box"> <span>Subscribe now </span> <svg aria-hidden="true" focusable="false" width="16" height="16" class="u-icon"> <use xlink:href="#icon-eds-i-arrow-right-medium"></use> </svg></a> </div> <h2 class="springer-plus-heading-end">Buy Now</h2> </div> <div class="c-box"> <div class="buying-options"> <div class="buying-option expanded"> <dl class="buying-option-price"> <dt> <svg width="24" height="24" xmlns="http://www.w3.org/2000/svg" aria-hidden="true" focusable="false"> <path d="M11.782 11L9.3 8.518c-.393-.392-.4-1.022-.02-1.403a1.001 1.001 0 011.417 0l4.176 4.177a1.001 1.001 0 010 1.416l-4.176 4.177a.991.991 0 01-1.4.016 1 1 0 01.003-1.42L11.782 13l1.013-.998L11.782 11z" fill="#666" fill-rule="evenodd"></path> </svg> Protocol </dt> <dd class="price-amount"> <div data-test-id="test-chapter-price" class="buybox__price"> EUR 44.95 </div> </dd> <dd class="price-info"> Price includes VAT (Hong Kong/P.R.China) </dd> </dl> <form class="buying-option-form" action="https://order.springer.com/public/cart" method="post"> <input type="hidden" name="type" value="protocol"> <input type="hidden" name="doi" value="10.1007/978-1-0716-4108-8_22"> <input type="hidden" name="isxn" value="978-1-0716-4108-8"> <input type="hidden" name="contenttitle" value="Zebrafish as a Model for Investigating Antiviral Innate Immunity"> <input type="hidden" name="copyrightyear" value="2025"> <input type="hidden" name="year" value="2024"> <input type="hidden" name="authors" value="Xiao Man Wu, Si Yao Zheng, Ming Xian Chang"> <input type="hidden" name="title" value="Antiviral Innate Immunity"> <input type="hidden" name="mac" value="de422a41bdb749b7a4b08cd933940f3d"> <ul class="buying-option-usps"> <li>Available as PDF</li> <li>Read on any device</li> <li>Instant download</li> <li>Own it forever</li> </ul> <button type="submit" class="u-button u-button--full-width u-button--primary u-button--xsmall" value="Submit" data-track="click" data-track-prefer="click" data-track-action="buy pdf" data-track-label="buy chapter action" onclick="dataLayer.push({"event":"addToCart","ecommerce":{"currencyCode":"EUR","add":{"products":[{"name":"Zebrafish as a Model for Investigating Antiviral Innate Immunity","id":"10.1007/978-1-0716-4108-8_22","price":44.95,"brand":"Springer US","category":"Innate Immunity","variant":"ppv-protocol","quantity":1}]}}});">Buy Protocol <svg aria-hidden="true" focusable="false" width="16" height="16" class="u-icon"> <use xlink:href="#icon-eds-i-arrow-right-medium"></use> </svg></button> </form> </div> <div class="buying-option expanded"> <dl class="buying-option-price"> <dt> <svg width="24" height="24" xmlns="http://www.w3.org/2000/svg" aria-hidden="true" focusable="false"> <path d="M11.782 11L9.3 8.518c-.393-.392-.4-1.022-.02-1.403a1.001 1.001 0 011.417 0l4.176 4.177a1.001 1.001 0 010 1.416l-4.176 4.177a.991.991 0 01-1.4.016 1 1 0 01.003-1.42L11.782 13l1.013-.998L11.782 11z" fill="#666" fill-rule="evenodd"></path> </svg> eBook </dt> <dd class="price-amount"> EUR 171.19 </dd> <dd class="price-info"> Price includes VAT (Hong Kong/P.R.China) </dd> </dl> <form class="buying-option-form" action="https://order.springer.com/public/cart" method="post"> <input type="hidden" name="type" value="ebook"> <input type="hidden" name="doi" value="10.1007/978-1-0716-4108-8"> <input type="hidden" name="isxn" value="978-1-0716-4108-8"> <input type="hidden" name="contenttitle" value="Antiviral Innate Immunity"> <input type="hidden" name="mac" value="950e5c903ca87eceec05dfe3573d969e"> <ul class="buying-option-usps"> <li>Available as EPUB and PDF</li> <li>Read on any device</li> <li>Instant download</li> <li>Own it forever</li> </ul> <button type="submit" class="u-button u-button--full-width u-button--primary u-button--xsmall" value="Submit" data-track="click" data-track-prefer="click" data-track-label="buy ebook" onclick="dataLayer.push({"event":"addToCart","ecommerce":{"currencyCode":"EUR","add":{"products":[{"name":"Antiviral Innate Immunity","id":"978-1-0716-4108-8","price":149.99,"brand":"Springer US","category":"Innate Immunity","variant":"ebo","quantity":1}]}}});">Buy eBook <svg aria-hidden="true" focusable="false" width="16" height="16" class="u-icon"> <use xlink:href="#icon-eds-i-arrow-right-medium"></use> </svg></button> </form> </div> <div class="buying-option expanded"> <dl class="buying-option-price"> <dt> <svg width="24" height="24" xmlns="http://www.w3.org/2000/svg" aria-hidden="true" focusable="false"> <path d="M11.782 11L9.3 8.518c-.393-.392-.4-1.022-.02-1.403a1.001 1.001 0 011.417 0l4.176 4.177a1.001 1.001 0 010 1.416l-4.176 4.177a.991.991 0 01-1.4.016 1 1 0 01.003-1.42L11.782 13l1.013-.998L11.782 11z" fill="#666" fill-rule="evenodd"></path> </svg> Hardcover Book </dt> <dd class="price-amount"> EUR 219.99 </dd> <dd class="price-info"> Price excludes VAT (Hong Kong/P.R.China) </dd> </dl> <form class="buying-option-form" action="https://order.springer.com/public/cart" method="post"> <input type="hidden" name="type" value="book"> <input type="hidden" name="doi" value="10.1007/978-1-0716-4108-8"> <input type="hidden" name="isxn" value="978-1-0716-4107-1"> <input type="hidden" name="contenttitle" value="Antiviral Innate Immunity"> <input type="hidden" name="mac" value="c0f426b571edd1d0e4fb7c0fc8cb93f9"> <ul class="buying-option-usps"> <li>Durable hardcover edition</li> <li>Dispatched in 3 to 5 business days</li> <li>Free shipping worldwide - <a href="https://support.springernature.com/en/support/solutions/articles/6000233448-coronavirus-disease-covid-19-delivery-information" target="_blank">see info</a></li> </ul> <button type="submit" class="u-button u-button--full-width u-button--primary u-button--xsmall" value="Submit" data-track="click" data-track-prefer="click" data-track-label="buy hardcover" onclick="dataLayer.push({"event":"addToCart","ecommerce":{"currencyCode":"EUR","add":{"products":[{"name":"Antiviral Innate Immunity","id":"978-1-0716-4107-1","price":219.99,"brand":"Springer US","category":"Innate Immunity","variant":"print","quantity":1}]}}});">Buy Hardcover Book <svg aria-hidden="true" focusable="false" width="16" height="16" class="u-icon"> <use xlink:href="#icon-eds-i-arrow-right-medium"></use> </svg></button> </form> </div> </div> </div> <div class="buybox-tax-info"> <p class="c-notes__text tax-info">Tax calculation will be finalised at checkout</p> <p class="c-notes__text buybox-additional-info">Purchases are for personal use only</p> </div> </div> <style> .c-springer-plus { display: none; } .springer-plus { background-color: #EBF6FF; padding: 16px; font-family: "Merriweather Sans", "Helvetica Neue", Helvetica, Arial, sans-serif; } .springer-plus-headline { display: flex; justify-content: space-between; } .springer-plus-heading { border-bottom: 1px solid #c5e0f4; border-top: 1px solid #c5e0f4; font-family: "Merriweather Sans", "Helvetica Neue", Helvetica, Arial, sans-serif; font-size: 1.125rem; text-align: center; font-weight: 700; padding: 16px; margin: 0; } .springer-plus-heading-end { border-top: 1px solid #c5e0f4; font-family: "Merriweather Sans", "Helvetica Neue", Helvetica, Arial, sans-serif; font-size: 1.125rem; text-align: center; font-weight: 700; padding: 16px; margin: 0; } .springer-plus-title { display: flex; align-items: center; } .springer-plus-title span { margin-left: 8px; } .sprcom-buybox-darwin-b .springer-plus a { color: #025e8d; font-size: 16px; background-color: #fff; border: 1px solid #025e8d; font-weight: 700; max-height: 44px; } .sprcom-buybox-darwin-b .springer-plus a span { margin-right: 8px; } .sprcom-buybox-darwin-b .springer-plus a:hover { color: #fff; background-color: #025e8d; border: 4px solid #025e8d; box-shadow: none; font-weight: 700; } .sprcom-buybox-darwin-b .springer-plus a:visited { color: #025e8d; } .sprcom-buybox-darwin-b .springer-plus a:visited:hover { color: #fff; } .sprcom-buybox-darwin-b .springer-plus .buying-option-usps { color: #555; font-size: 1rem; line-height: 1.6; margin: 0; padding-left: 0; list-style: none; padding-top: 16px; padding-bottom: 24px; border-top: 0; } /* end of springer-plus */ .sprcom-buybox-darwin-b > div { flex-grow: 1; width: 100%; } .sprcom-buybox-darwin-b .buying-options { display: flex; flex-wrap: wrap; margin-top: 0; } .sprcom-buybox-darwin-b .buying-options > * { background-color: #F0F7FC; flex-grow: 1; flex-basis: auto; width: 360px; display: flex; flex-direction: column; justify-content: space-between; border-bottom: 1px solid #cedbe0; border-top: 1px solid #cedbe0; margin-top: -1px; } .sprcom-buybox-darwin-b dt { align-items: center; display: flex; font-weight: 700; margin-left: -10px; } .sprcom-buybox-darwin-b .buying-option-form { padding: 0 16px 16px; } .sprcom-buybox-darwin-b .buying-option-form button { gap: 8px; margin: 20px 0px; } .sprcom-buybox-darwin-b .buying-option-price { align-items: center; display: flex; flex-wrap: wrap; font-size: 1rem; line-height: 1.4; user-select: none; cursor: pointer; padding: 24px; margin: 0; } .sprcom-buybox-darwin-b .buying-option-price:focus { outline: 4px solid #08c; z-index: 1; } .sprcom-buybox-darwin-b .buying-option-price dt, .sprcom-buybox-darwin-b .buying-option-price dt svg path { color: #025e8d; fill: #025e8d; } .sprcom-buybox-darwin-b .buying-option-price dt, .sprcom-buybox-darwin-b .buying-option-price dd { flex-grow: 1; } .sprcom-buybox-darwin-b .buying-option-price dt svg { height: auto; min-width: 24px; margin-right: 12px; } .sprcom-buybox-darwin-b .buying-option-price .price-info { color: #555; font-size: .875rem; text-align: right; width: 100%; } .sprcom-buybox-darwin-b .buying-option-price .price-amount { color: #555; text-align: right; font-weight: 600; } .sprcom-buybox-darwin-b .buying-option-price .price-amount-without-discount { color: #c40606; text-decoration: line-through; width: 100%; } .sprcom-buybox-darwin-b .buying-option-price .price-type { font-size: 40%; margin-left: 8px; } .sprcom-buybox-darwin-b .buying-option-usps { color: #555; font-size: 1rem; line-height: 1.6; margin: 0; padding-left: 0; list-style: none; padding-top: 16px; border-top: 1px solid #f0f0f0; } .sprcom-buybox-darwin-b .buying-option-usps > li { position: relative; padding-left: 26px; } .sprcom-buybox-darwin-b .buying-option-usps > li::before { position: absolute; content: ""; left: 0; top: calc(0.8em - 5px); background-image: url("data:image/svg+xml,%3Csvg viewBox='0 0 100 100' xmlns='http://www.w3.org/2000/svg' fill='%230070A8'%3E%3Ccircle cx='50' cy='50' r='50'/%3E%3C/svg%3E"); width: 10px; height: 10px; } .sprcom-buybox-darwin-b .buying-option-usps > li:not(:first-child) { margin-top: 4px; } .sprcom-buybox-darwin-b .buying-options > .expanded { background-color: #fff; } .sprcom-buybox-darwin-b dl { } .sprcom-buybox-darwin-b a:visited { color: #004b83; } .sprcom-buybox-darwin-b [aria-expanded=false] svg { transform: rotate(90deg) scale(1.5); } .sprcom-buybox-darwin-b [aria-expanded=true] svg { transform: rotate(270deg) scale(1.5); } .sprcom-buybox-darwin-b dt { align-items: center; display: flex; } .sprcom-buybox-darwin-b style { display: none; } .sprcom-buybox-darwin-b .buybox-tax-info { text-align: center; padding: 16px; } .sprcom-buybox-darwin-b .tax-info, .sprcom-buybox-darwin-b .buybox-additional-info { font-size: .875rem; } .sprcom-buybox-darwin-b .buybox-additional-info { font-weight: 600; } .sprcom-buybox-darwin-b .u-button--primary.u-button--xsmall { font-size: .875rem; padding: 2px 8px; } </style> <script> ;(function () { var timestamp = Date.now() document.write('<div data-id="id_'+ timestamp +'"></div>') var buybox = document.querySelector("[data-id=id_"+ timestamp +"]").parentNode var buyingOptions = buybox.querySelectorAll(".buying-option") ;[].slice.call(buyingOptions).forEach(initCollapsibles) // springerPlus roll out 10% starts here var springerPlusGroup = setLocalStorageSpringerPlus(); var rollOutSpringerPlus = springerPlusGroup === "B" function setLocalStorageSpringerPlus() { var selectUserKey = "springerPlusRollOut"; var springerPlusGroup = "X"; if (!window.localStorage) return springerPlusGroup; try { var selectUserValue = window.localStorage.getItem(selectUserKey) springerPlusGroup = selectUserValue || randomDistributionSpringerPlus(selectUserKey) } catch (err) { console.log(err) } return springerPlusGroup; } function randomDistributionSpringerPlus(selectUserKey) { var randomGroup = Math.random() < 0.9 ? "A" : "B" window.localStorage.setItem(selectUserKey, randomGroup) return randomGroup } if (rollOutSpringerPlus) { revealSpringerPlus(); } function revealSpringerPlus() { if(buybox) { document.querySelectorAll(".c-springer-plus").forEach(function(node) { node.style.display = "block" }) } } //springerPlus ends here var buyboxMaxSingleColumnWidth = 480 function initCollapsibles(subscription, index) { var toggle = subscription.querySelector(".buying-option-price") subscription.classList.remove("expanded") var form = subscription.querySelector(".buying-option-form") var priceInfo = subscription.querySelector(".price-info") var buyingOption = toggle.parentElement if (toggle && form && priceInfo) { toggle.setAttribute("role", "button") toggle.setAttribute("tabindex", "0") toggle.addEventListener("click", function (event) { var expandedBuyingOptions = buybox.querySelectorAll(".buying-option.expanded") var buyboxWidth = buybox.offsetWidth ;[].slice.call(expandedBuyingOptions).forEach(function(option) { if (buyboxWidth <= buyboxMaxSingleColumnWidth && option != buyingOption) { hideBuyingOption(option) } }) var expanded = toggle.getAttribute("aria-expanded") === "true" || false toggle.setAttribute("aria-expanded", !expanded) form.hidden = expanded if (!expanded) { buyingOption.classList.add("expanded") } else { buyingOption.classList.remove("expanded") } priceInfo.hidden = expanded }, false) } } function hideBuyingOption(buyingOption) { var toggle = buyingOption.querySelector(".buying-option-price") var form = buyingOption.querySelector(".buying-option-form") var priceInfo = buyingOption.querySelector(".price-info") toggle.setAttribute("aria-expanded", false) form.hidden = true buyingOption.classList.remove("expanded") priceInfo.hidden = true } function initKeyControls() { document.addEventListener("keydown", function (event) { if (document.activeElement.classList.contains("buying-option-price") && (event.code === "Space" || event.code === "Enter")) { if (document.activeElement) { event.preventDefault() document.activeElement.click() } } }, false) } function initialStateOpen() { var buyboxWidth = buybox.offsetWidth ;[].slice.call(buybox.querySelectorAll(".buying-option")).forEach(function (option, index) { var toggle = option.querySelector(".buying-option-price") var form = option.querySelector(".buying-option-form") var priceInfo = option.querySelector(".price-info") if (buyboxWidth > buyboxMaxSingleColumnWidth) { toggle.click() } else { if (index === 0) { toggle.click() } else { toggle.setAttribute("aria-expanded", "false") form.hidden = "hidden" priceInfo.hidden = "hidden" } } }) } initialStateOpen() if (window.buyboxInitialised) return window.buyboxInitialised = true initKeyControls() })() </script> <script> ;(function () { if (document.cookie.indexOf("feature-monetise-subscriptions-display-springer-plus") > -1) { document.querySelectorAll(".c-springer-plus").forEach(function(node) { node.style.display = "block" }) } })() </script> </div> </div> <div class="app-article-access__subscriptions"> <p><a href="https://www.springernature.com/gp/librarians/licensing/agc/ebooks">Institutional subscriptions <svg aria-hidden="true" focusable="false" width="24" height="24" class="u-icon"> <use xlink:href="#icon-eds-i-arrow-right-medium"></use> </svg> </a></p> </div> </div> <div class="c-reading-companion__panel c-reading-companion__sections c-reading-companion__panel--active" id="tabpanel-sections"></div> <div class="c-reading-companion__panel c-reading-companion__figures c-reading-companion__panel--full-width" id="tabpanel-figures"></div> <div class="c-reading-companion__panel c-reading-companion__references c-reading-companion__panel--full-width" id="tabpanel-references"></div> </div> </div> </aside> </div> </div> <div class="app-elements"> <div class="eds-c-header__expander eds-c-header__expander--search" id="eds-c-header-popup-search"> <h2 class="eds-c-header__heading">Search</h2> <div class="u-container"> <search class="eds-c-header__search" role="search" aria-label="Search from the header"> <form method="GET" action="//link.springer.com/search" data-test="header-search" data-track="search" data-track-context="search from header" data-track-action="submit search form" data-track-category="unified header" data-track-label="form" > <label for="eds-c-header-search" class="eds-c-header__search-label">Search by keyword or author</label> <div class="eds-c-header__search-container"> <input id="eds-c-header-search" class="eds-c-header__search-input" autocomplete="off" name="query" type="search" value="" required> <button class="eds-c-header__search-button" type="submit"> <svg class="eds-c-header__icon" aria-hidden="true" focusable="false"> <use xlink:href="#icon-eds-i-search-medium"></use> </svg> <span class="u-visually-hidden">Search</span> </button> </div> </form> </search> </div> </div> <div class="eds-c-header__expander eds-c-header__expander--menu" id="eds-c-header-nav"> <h2 class="eds-c-header__heading">Navigation</h2> <ul class="eds-c-header__list"> <li class="eds-c-header__list-item"> <a class="eds-c-header__link" href="https://link.springer.com/journals/" data-track="nav_find_a_journal" data-track-context="unified header" data-track-action="click find a journal" data-track-category="unified header" data-track-label="link" > Find a journal </a> </li> <li class="eds-c-header__list-item"> <a class="eds-c-header__link" href="https://www.springernature.com/gp/authors" data-track="nav_how_to_publish" data-track-context="unified header" data-track-action="click publish with us link" data-track-category="unified header" data-track-label="link" > Publish with us </a> </li> <li class="eds-c-header__list-item"> <a class="eds-c-header__link" href="https://link.springernature.com/home/" data-track="nav_track_your_research" data-track-context="unified header" data-track-action="click track your research" data-track-category="unified header" data-track-label="link" > Track your research </a> </li> </ul> </div> <footer > <div class="eds-c-footer" > <div class="eds-c-footer__container"> <div class="eds-c-footer__grid eds-c-footer__group--separator"> <div class="eds-c-footer__group"> <h3 class="eds-c-footer__heading">Discover content</h3> <ul class="eds-c-footer__list"> <li class="eds-c-footer__item"><a class="eds-c-footer__link" href="https://link.springer.com/journals/a/1" data-track="nav_journals_a_z" data-track-action="journals a-z" data-track-context="unified footer" data-track-label="link">Journals A-Z</a></li> <li class="eds-c-footer__item"><a class="eds-c-footer__link" href="https://link.springer.com/books/a/1" data-track="nav_books_a_z" data-track-action="books a-z" data-track-context="unified footer" data-track-label="link">Books A-Z</a></li> </ul> </div> <div class="eds-c-footer__group"> <h3 class="eds-c-footer__heading">Publish with us</h3> <ul class="eds-c-footer__list"> <li class="eds-c-footer__item"><a class="eds-c-footer__link" href="https://link.springer.com/journals" data-track="nav_journal_finder" data-track-action="journal finder" data-track-context="unified footer" data-track-label="link">Journal finder</a></li> <li class="eds-c-footer__item"><a class="eds-c-footer__link" href="https://www.springernature.com/gp/authors" data-track="nav_publish_your_research" data-track-action="publish your research" data-track-context="unified footer" data-track-label="link">Publish your research</a></li> <li class="eds-c-footer__item"><a class="eds-c-footer__link" href="https://www.springernature.com/gp/open-research/about/the-fundamentals-of-open-access-and-open-research" data-track="nav_open_access_publishing" data-track-action="open access publishing" data-track-context="unified footer" data-track-label="link">Open access publishing</a></li> </ul> </div> <div class="eds-c-footer__group"> <h3 class="eds-c-footer__heading">Products and services</h3> <ul class="eds-c-footer__list"> <li class="eds-c-footer__item"><a class="eds-c-footer__link" href="https://www.springernature.com/gp/products" data-track="nav_our_products" data-track-action="our products" data-track-context="unified footer" data-track-label="link">Our products</a></li> <li class="eds-c-footer__item"><a class="eds-c-footer__link" href="https://www.springernature.com/gp/librarians" data-track="nav_librarians" data-track-action="librarians" data-track-context="unified footer" data-track-label="link">Librarians</a></li> <li class="eds-c-footer__item"><a class="eds-c-footer__link" href="https://www.springernature.com/gp/societies" data-track="nav_societies" data-track-action="societies" data-track-context="unified footer" data-track-label="link">Societies</a></li> <li class="eds-c-footer__item"><a class="eds-c-footer__link" href="https://www.springernature.com/gp/partners" data-track="nav_partners_and_advertisers" data-track-action="partners and advertisers" data-track-context="unified footer" data-track-label="link">Partners and advertisers</a></li> </ul> </div> <div class="eds-c-footer__group"> <h3 class="eds-c-footer__heading">Our imprints</h3> <ul class="eds-c-footer__list"> <li class="eds-c-footer__item"><a class="eds-c-footer__link" href="https://www.springer.com/" data-track="nav_imprint_Springer" data-track-action="Springer" data-track-context="unified footer" data-track-label="link">Springer</a></li> <li class="eds-c-footer__item"><a class="eds-c-footer__link" href="https://www.nature.com/" data-track="nav_imprint_Nature_Portfolio" data-track-action="Nature Portfolio" data-track-context="unified footer" data-track-label="link">Nature Portfolio</a></li> <li class="eds-c-footer__item"><a class="eds-c-footer__link" href="https://www.biomedcentral.com/" data-track="nav_imprint_BMC" data-track-action="BMC" data-track-context="unified footer" data-track-label="link">BMC</a></li> <li class="eds-c-footer__item"><a class="eds-c-footer__link" href="https://www.palgrave.com/" data-track="nav_imprint_Palgrave_Macmillan" data-track-action="Palgrave Macmillan" data-track-context="unified footer" data-track-label="link">Palgrave Macmillan</a></li> <li class="eds-c-footer__item"><a class="eds-c-footer__link" href="https://www.apress.com/" data-track="nav_imprint_Apress" data-track-action="Apress" data-track-context="unified footer" data-track-label="link">Apress</a></li> </ul> </div> </div> </div> <div class="eds-c-footer__container"> <nav aria-label="footer navigation"> <ul class="eds-c-footer__links"> <li class="eds-c-footer__item"> <button class="eds-c-footer__link" data-cc-action="preferences" data-track="dialog_manage_cookies" data-track-action="Manage cookies" data-track-context="unified footer" data-track-label="link"><span class="eds-c-footer__button-text">Your privacy choices/Manage cookies</span></button> </li> <li class="eds-c-footer__item"> <a class="eds-c-footer__link" href="https://www.springernature.com/gp/legal/ccpa" data-track="nav_california_privacy_statement" data-track-action="california privacy statement" data-track-context="unified footer" data-track-label="link">Your US state privacy rights</a> </li> <li class="eds-c-footer__item"> <a class="eds-c-footer__link" href="https://www.springernature.com/gp/info/accessibility" data-track="nav_accessibility_statement" data-track-action="accessibility statement" data-track-context="unified footer" data-track-label="link">Accessibility statement</a> </li> <li class="eds-c-footer__item"> <a class="eds-c-footer__link" href="https://link.springer.com/termsandconditions" data-track="nav_terms_and_conditions" data-track-action="terms and conditions" data-track-context="unified footer" data-track-label="link">Terms and conditions</a> </li> <li class="eds-c-footer__item"> <a class="eds-c-footer__link" href="https://link.springer.com/privacystatement" data-track="nav_privacy_policy" data-track-action="privacy policy" data-track-context="unified footer" data-track-label="link">Privacy policy</a> </li> <li class="eds-c-footer__item"> <a class="eds-c-footer__link" href="https://support.springernature.com/en/support/home" data-track="nav_help_and_support" data-track-action="help and support" data-track-context="unified footer" data-track-label="link">Help and support</a> </li> <li class="eds-c-footer__item"> <a class="eds-c-footer__link" href="https://support.springernature.com/en/support/solutions/articles/6000255911-subscription-cancellations" data-track-action="cancel contracts here">Cancel contracts here</a> </li> </ul> </nav> <div class="eds-c-footer__user"> <p class="eds-c-footer__user-info"> <span data-test="footer-user-ip">8.222.208.146</span> </p> <p class="eds-c-footer__user-info" data-test="footer-business-partners">Not affiliated</p> </div> <a href="https://www.springernature.com/" class="eds-c-footer__link"> <img src="/oscar-static/images/logo-springernature-white-19dd4ba190.svg" alt="Springer Nature" loading="lazy" width="200" height="20"/> </a> <p class="eds-c-footer__legal" data-test="copyright">© 2024 Springer Nature</p> </div> </div> </footer> </div> </div> <div class="u-visually-hidden" aria-hidden="true" data-test="darwin-icons"> <?xml version="1.0" encoding="UTF-8"?><!DOCTYPE svg PUBLIC "-//W3C//DTD SVG 1.1//EN" "http://www.w3.org/Graphics/SVG/1.1/DTD/svg11.dtd"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><symbol id="icon-eds-i-accesses-medium" viewBox="0 0 24 24"><path d="M15.59 1a1 1 0 0 1 .706.291l5.41 5.385a1 1 0 0 1 .294.709v13.077c0 .674-.269 1.32-.747 1.796a2.549 2.549 0 0 1-1.798.742H15a1 1 0 0 1 0-2h4.455a.549.549 0 0 0 .387-.16.535.535 0 0 0 .158-.378V7.8L15.178 3H5.545a.543.543 0 0 0-.538.451L5 3.538v8.607a1 1 0 0 1-2 0V3.538A2.542 2.542 0 0 1 5.545 1h10.046ZM8 13c2.052 0 4.66 1.61 6.36 3.4l.124.141c.333.41.516.925.516 1.459 0 .6-.232 1.178-.64 1.599C12.666 21.388 10.054 23 8 23c-2.052 0-4.66-1.61-6.353-3.393A2.31 2.31 0 0 1 1 18c0-.6.232-1.178.64-1.6C3.34 14.61 5.948 13 8 13Zm0 2c-1.369 0-3.552 1.348-4.917 2.785A.31.31 0 0 0 3 18c0 .083.031.161.09.222C4.447 19.652 6.631 21 8 21c1.37 0 3.556-1.35 4.917-2.785A.31.31 0 0 0 13 18a.32.32 0 0 0-.048-.17l-.042-.052C11.553 16.348 9.369 15 8 15Zm0 1a2 2 0 1 1 0 4 2 2 0 0 1 0-4Z"/></symbol><symbol id="icon-eds-i-altmetric-medium" viewBox="0 0 24 24"><path d="M12 1c5.978 0 10.843 4.77 10.996 10.712l.004.306-.002.022-.002.248C22.843 18.23 17.978 23 12 23 5.925 23 1 18.075 1 12S5.925 1 12 1Zm-1.726 9.246L8.848 12.53a1 1 0 0 1-.718.461L8.003 13l-4.947.014a9.001 9.001 0 0 0 17.887-.001L16.553 13l-2.205 3.53a1 1 0 0 1-1.735-.068l-.05-.11-2.289-6.106ZM12 3a9.001 9.001 0 0 0-8.947 8.013l4.391-.012L9.652 7.47a1 1 0 0 1 1.784.179l2.288 6.104 1.428-2.283a1 1 0 0 1 .722-.462l.129-.008 4.943.012A9.001 9.001 0 0 0 12 3Z"/></symbol><symbol id="icon-eds-i-arrow-bend-down-medium" viewBox="0 0 24 24"><path d="m11.852 20.989.058.007L12 21l.075-.003.126-.017.111-.03.111-.044.098-.052.104-.074.082-.073 6-6a1 1 0 0 0-1.414-1.414L13 17.585v-12.2C13 4.075 11.964 3 10.667 3H4a1 1 0 1 0 0 2h6.667c.175 0 .333.164.333.385v12.2l-4.293-4.292a1 1 0 0 0-1.32-.083l-.094.083a1 1 0 0 0 0 1.414l6 6c.035.036.073.068.112.097l.11.071.114.054.105.035.118.025Z"/></symbol><symbol id="icon-eds-i-arrow-bend-down-small" viewBox="0 0 16 16"><path d="M1 2a1 1 0 0 0 1 1h5v8.585L3.707 8.293a1 1 0 0 0-1.32-.083l-.094.083a1 1 0 0 0 0 1.414l5 5 .063.059.093.069.081.048.105.048.104.035.105.022.096.01h.136l.122-.018.113-.03.103-.04.1-.053.102-.07.052-.043 5.04-5.037a1 1 0 1 0-1.415-1.414L9 11.583V3a2 2 0 0 0-2-2H2a1 1 0 0 0-1 1Z"/></symbol><symbol id="icon-eds-i-arrow-bend-up-medium" viewBox="0 0 24 24"><path d="m11.852 3.011.058-.007L12 3l.075.003.126.017.111.03.111.044.098.052.104.074.082.073 6 6a1 1 0 1 1-1.414 1.414L13 6.415v12.2C13 19.925 11.964 21 10.667 21H4a1 1 0 0 1 0-2h6.667c.175 0 .333-.164.333-.385v-12.2l-4.293 4.292a1 1 0 0 1-1.32.083l-.094-.083a1 1 0 0 1 0-1.414l6-6c.035-.036.073-.068.112-.097l.11-.071.114-.054.105-.035.118-.025Z"/></symbol><symbol id="icon-eds-i-arrow-bend-up-small" viewBox="0 0 16 16"><path d="M1 13.998a1 1 0 0 1 1-1h5V4.413L3.707 7.705a1 1 0 0 1-1.32.084l-.094-.084a1 1 0 0 1 0-1.414l5-5 .063-.059.093-.068.081-.05.105-.047.104-.035.105-.022L7.94 1l.136.001.122.017.113.03.103.04.1.053.102.07.052.043 5.04 5.037a1 1 0 1 1-1.415 1.414L9 4.415v8.583a2 2 0 0 1-2 2H2a1 1 0 0 1-1-1Z"/></symbol><symbol id="icon-eds-i-arrow-diagonal-medium" viewBox="0 0 24 24"><path d="M14 3h6l.075.003.126.017.111.03.111.044.098.052.096.067.09.08c.036.035.068.073.097.112l.071.11.054.114.035.105.03.148L21 4v6a1 1 0 0 1-2 0V6.414l-4.293 4.293a1 1 0 0 1-1.414-1.414L17.584 5H14a1 1 0 0 1-.993-.883L13 4a1 1 0 0 1 1-1ZM4 13a1 1 0 0 1 1 1v3.584l4.293-4.291a1 1 0 1 1 1.414 1.414L6.414 19H10a1 1 0 0 1 .993.883L11 20a1 1 0 0 1-1 1l-6.075-.003-.126-.017-.111-.03-.111-.044-.098-.052-.096-.067-.09-.08a1.01 1.01 0 0 1-.097-.112l-.071-.11-.054-.114-.035-.105-.025-.118-.007-.058L3 20v-6a1 1 0 0 1 1-1Z"/></symbol><symbol id="icon-eds-i-arrow-diagonal-small" viewBox="0 0 16 16"><path d="m2 15-.082-.004-.119-.016-.111-.03-.111-.044-.098-.052-.096-.067-.09-.08a1.008 1.008 0 0 1-.097-.112l-.071-.11-.031-.062-.034-.081-.024-.076-.025-.118-.007-.058L1 14.02V9a1 1 0 1 1 2 0v2.584l2.793-2.791a1 1 0 1 1 1.414 1.414L4.414 13H7a1 1 0 0 1 .993.883L8 14a1 1 0 0 1-1 1H2ZM14 1l.081.003.12.017.111.03.111.044.098.052.096.067.09.08c.036.035.068.073.097.112l.071.11.031.062.034.081.024.076.03.148L15 2v5a1 1 0 0 1-2 0V4.414l-2.96 2.96A1 1 0 1 1 8.626 5.96L11.584 3H9a1 1 0 0 1-.993-.883L8 2a1 1 0 0 1 1-1h5Z"/></symbol><symbol id="icon-eds-i-arrow-down-medium" viewBox="0 0 24 24"><path d="m20.707 12.728-7.99 7.98a.996.996 0 0 1-.561.281l-.157.011a.998.998 0 0 1-.788-.384l-7.918-7.908a1 1 0 0 1 1.414-1.416L11 17.576V4a1 1 0 0 1 2 0v13.598l6.293-6.285a1 1 0 0 1 1.32-.082l.095.083a1 1 0 0 1-.001 1.414Z"/></symbol><symbol id="icon-eds-i-arrow-down-small" viewBox="0 0 16 16"><path d="m1.293 8.707 6 6 .063.059.093.069.081.048.105.049.104.034.056.013.118.017L8 15l.076-.003.122-.017.113-.03.085-.032.063-.03.098-.058.06-.043.05-.043 6.04-6.037a1 1 0 0 0-1.414-1.414L9 11.583V2a1 1 0 1 0-2 0v9.585L2.707 7.293a1 1 0 0 0-1.32-.083l-.094.083a1 1 0 0 0 0 1.414Z"/></symbol><symbol id="icon-eds-i-arrow-left-medium" viewBox="0 0 24 24"><path d="m11.272 3.293-7.98 7.99a.996.996 0 0 0-.281.561L3 12.001c0 .32.15.605.384.788l7.908 7.918a1 1 0 0 0 1.416-1.414L6.424 13H20a1 1 0 0 0 0-2H6.402l6.285-6.293a1 1 0 0 0 .082-1.32l-.083-.095a1 1 0 0 0-1.414.001Z"/></symbol><symbol id="icon-eds-i-arrow-left-small" viewBox="0 0 16 16"><path d="m7.293 1.293-6 6-.059.063-.069.093-.048.081-.049.105-.034.104-.013.056-.017.118L1 8l.003.076.017.122.03.113.032.085.03.063.058.098.043.06.043.05 6.037 6.04a1 1 0 0 0 1.414-1.414L4.417 9H14a1 1 0 0 0 0-2H4.415l4.292-4.293a1 1 0 0 0 .083-1.32l-.083-.094a1 1 0 0 0-1.414 0Z"/></symbol><symbol id="icon-eds-i-arrow-right-medium" viewBox="0 0 24 24"><path d="m12.728 3.293 7.98 7.99a.996.996 0 0 1 .281.561l.011.157c0 .32-.15.605-.384.788l-7.908 7.918a1 1 0 0 1-1.416-1.414L17.576 13H4a1 1 0 0 1 0-2h13.598l-6.285-6.293a1 1 0 0 1-.082-1.32l.083-.095a1 1 0 0 1 1.414.001Z"/></symbol><symbol id="icon-eds-i-arrow-right-small" viewBox="0 0 16 16"><path d="m8.707 1.293 6 6 .059.063.069.093.048.081.049.105.034.104.013.056.017.118L15 8l-.003.076-.017.122-.03.113-.032.085-.03.063-.058.098-.043.06-.043.05-6.037 6.04a1 1 0 0 1-1.414-1.414L11.583 9H2a1 1 0 1 1 0-2h9.585L7.293 2.707a1 1 0 0 1-.083-1.32l.083-.094a1 1 0 0 1 1.414 0Z"/></symbol><symbol id="icon-eds-i-arrow-up-medium" viewBox="0 0 24 24"><path d="m3.293 11.272 7.99-7.98a.996.996 0 0 1 .561-.281L12.001 3c.32 0 .605.15.788.384l7.918 7.908a1 1 0 0 1-1.414 1.416L13 6.424V20a1 1 0 0 1-2 0V6.402l-6.293 6.285a1 1 0 0 1-1.32.082l-.095-.083a1 1 0 0 1 .001-1.414Z"/></symbol><symbol id="icon-eds-i-arrow-up-small" viewBox="0 0 16 16"><path d="m1.293 7.293 6-6 .063-.059.093-.069.081-.048.105-.049.104-.034.056-.013.118-.017L8 1l.076.003.122.017.113.03.085.032.063.03.098.058.06.043.05.043 6.04 6.037a1 1 0 0 1-1.414 1.414L9 4.417V14a1 1 0 0 1-2 0V4.415L2.707 8.707a1 1 0 0 1-1.32.083l-.094-.083a1 1 0 0 1 0-1.414Z"/></symbol><symbol id="icon-eds-i-article-medium" viewBox="0 0 24 24"><path d="M8 7a1 1 0 0 0 0 2h4a1 1 0 1 0 0-2H8ZM8 11a1 1 0 1 0 0 2h8a1 1 0 1 0 0-2H8ZM7 16a1 1 0 0 1 1-1h8a1 1 0 1 1 0 2H8a1 1 0 0 1-1-1Z"/><path d="M5.545 1A2.542 2.542 0 0 0 3 3.538v16.924A2.542 2.542 0 0 0 5.545 23h12.91A2.542 2.542 0 0 0 21 20.462V3.5A2.5 2.5 0 0 0 18.5 1H5.545ZM5 3.538C5 3.245 5.24 3 5.545 3H18.5a.5.5 0 0 1 .5.5v16.962c0 .293-.24.538-.546.538H5.545A.542.542 0 0 1 5 20.462V3.538Z" clip-rule="evenodd"/></symbol><symbol id="icon-eds-i-book-medium" viewBox="0 0 24 24"><path d="M18.5 1A2.5 2.5 0 0 1 21 3.5v12c0 1.16-.79 2.135-1.86 2.418l-.14.031V21h1a1 1 0 0 1 .993.883L21 22a1 1 0 0 1-1 1H6.5A3.5 3.5 0 0 1 3 19.5v-15A3.5 3.5 0 0 1 6.5 1h12ZM17 18H6.5a1.5 1.5 0 0 0-1.493 1.356L5 19.5A1.5 1.5 0 0 0 6.5 21H17v-3Zm1.5-15h-12A1.5 1.5 0 0 0 5 4.5v11.837l.054-.025a3.481 3.481 0 0 1 1.254-.307L6.5 16h12a.5.5 0 0 0 .492-.41L19 15.5v-12a.5.5 0 0 0-.5-.5ZM15 6a1 1 0 0 1 0 2H9a1 1 0 1 1 0-2h6Z"/></symbol><symbol id="icon-eds-i-book-series-medium" viewBox="0 0 24 24"><path fill-rule="evenodd" d="M1 3.786C1 2.759 1.857 2 2.82 2H6.18c.964 0 1.82.759 1.82 1.786V4h3.168c.668 0 1.298.364 1.616.938.158-.109.333-.195.523-.252l3.216-.965c.923-.277 1.962.204 2.257 1.187l4.146 13.82c.296.984-.307 1.957-1.23 2.234l-3.217.965c-.923.277-1.962-.203-2.257-1.187L13 10.005v10.21c0 1.04-.878 1.785-1.834 1.785H7.833c-.291 0-.575-.07-.83-.195A1.849 1.849 0 0 1 6.18 22H2.821C1.857 22 1 21.241 1 20.214V3.786ZM3 4v11h3V4H3Zm0 16v-3h3v3H3Zm15.075-.04-.814-2.712 2.874-.862.813 2.712-2.873.862Zm1.485-5.49-2.874.862-2.634-8.782 2.873-.862 2.635 8.782ZM8 20V6h3v14H8Z" clip-rule="evenodd"/></symbol><symbol id="icon-eds-i-calendar-acceptance-medium" viewBox="0 0 24 24"><path d="M17 2a1 1 0 0 1 1 1v1h1.5C20.817 4 22 5.183 22 6.5v13c0 1.317-1.183 2.5-2.5 2.5h-15C3.183 22 2 20.817 2 19.5v-13C2 5.183 3.183 4 4.5 4a1 1 0 1 1 0 2c-.212 0-.5.288-.5.5v13c0 .212.288.5.5.5h15c.212 0 .5-.288.5-.5v-13c0-.212-.288-.5-.5-.5H18v1a1 1 0 0 1-2 0V3a1 1 0 0 1 1-1Zm-.534 7.747a1 1 0 0 1 .094 1.412l-4.846 5.538a1 1 0 0 1-1.352.141l-2.77-2.076a1 1 0 0 1 1.2-1.6l2.027 1.519 4.236-4.84a1 1 0 0 1 1.411-.094ZM7.5 2a1 1 0 0 1 1 1v1H14a1 1 0 0 1 0 2H8.5v1a1 1 0 1 1-2 0V3a1 1 0 0 1 1-1Z"/></symbol><symbol id="icon-eds-i-calendar-date-medium" viewBox="0 0 24 24"><path d="M17 2a1 1 0 0 1 1 1v1h1.5C20.817 4 22 5.183 22 6.5v13c0 1.317-1.183 2.5-2.5 2.5h-15C3.183 22 2 20.817 2 19.5v-13C2 5.183 3.183 4 4.5 4a1 1 0 1 1 0 2c-.212 0-.5.288-.5.5v13c0 .212.288.5.5.5h15c.212 0 .5-.288.5-.5v-13c0-.212-.288-.5-.5-.5H18v1a1 1 0 0 1-2 0V3a1 1 0 0 1 1-1ZM8 15a1 1 0 1 1 0 2 1 1 0 0 1 0-2Zm4 0a1 1 0 1 1 0 2 1 1 0 0 1 0-2Zm-4-4a1 1 0 1 1 0 2 1 1 0 0 1 0-2Zm4 0a1 1 0 1 1 0 2 1 1 0 0 1 0-2Zm4 0a1 1 0 1 1 0 2 1 1 0 0 1 0-2ZM7.5 2a1 1 0 0 1 1 1v1H14a1 1 0 0 1 0 2H8.5v1a1 1 0 1 1-2 0V3a1 1 0 0 1 1-1Z"/></symbol><symbol id="icon-eds-i-calendar-decision-medium" viewBox="0 0 24 24"><path d="M17 2a1 1 0 0 1 1 1v1h1.5C20.817 4 22 5.183 22 6.5v13c0 1.317-1.183 2.5-2.5 2.5h-15C3.183 22 2 20.817 2 19.5v-13C2 5.183 3.183 4 4.5 4a1 1 0 1 1 0 2c-.212 0-.5.288-.5.5v13c0 .212.288.5.5.5h15c.212 0 .5-.288.5-.5v-13c0-.212-.288-.5-.5-.5H18v1a1 1 0 0 1-2 0V3a1 1 0 0 1 1-1Zm-2.935 8.246 2.686 2.645c.34.335.34.883 0 1.218l-2.686 2.645a.858.858 0 0 1-1.213-.009.854.854 0 0 1 .009-1.21l1.05-1.035H7.984a.992.992 0 0 1-.984-1c0-.552.44-1 .984-1h5.928l-1.051-1.036a.854.854 0 0 1-.085-1.121l.076-.088a.858.858 0 0 1 1.213-.009ZM7.5 2a1 1 0 0 1 1 1v1H14a1 1 0 0 1 0 2H8.5v1a1 1 0 1 1-2 0V3a1 1 0 0 1 1-1Z"/></symbol><symbol id="icon-eds-i-calendar-impact-factor-medium" viewBox="0 0 24 24"><path d="M17 2a1 1 0 0 1 1 1v1h1.5C20.817 4 22 5.183 22 6.5v13c0 1.317-1.183 2.5-2.5 2.5h-15C3.183 22 2 20.817 2 19.5v-13C2 5.183 3.183 4 4.5 4a1 1 0 1 1 0 2c-.212 0-.5.288-.5.5v13c0 .212.288.5.5.5h15c.212 0 .5-.288.5-.5v-13c0-.212-.288-.5-.5-.5H18v1a1 1 0 0 1-2 0V3a1 1 0 0 1 1-1Zm-3.2 6.924a.48.48 0 0 1 .125.544l-1.52 3.283h2.304c.27 0 .491.215.491.483a.477.477 0 0 1-.13.327l-4.18 4.484a.498.498 0 0 1-.69.031.48.48 0 0 1-.125-.544l1.52-3.284H9.291a.487.487 0 0 1-.491-.482c0-.121.047-.238.13-.327l4.18-4.484a.498.498 0 0 1 .69-.031ZM7.5 2a1 1 0 0 1 1 1v1H14a1 1 0 0 1 0 2H8.5v1a1 1 0 1 1-2 0V3a1 1 0 0 1 1-1Z"/></symbol><symbol id="icon-eds-i-call-papers-medium" viewBox="0 0 24 24"><g><path d="m20.707 2.883-1.414 1.414a1 1 0 0 0 1.414 1.414l1.414-1.414a1 1 0 0 0-1.414-1.414Z"/><path d="M6 16.054c0 2.026 1.052 2.943 3 2.943a1 1 0 1 1 0 2c-2.996 0-5-1.746-5-4.943v-1.227a4.068 4.068 0 0 1-1.83-1.189 4.553 4.553 0 0 1-.87-1.455 4.868 4.868 0 0 1-.3-1.686c0-1.17.417-2.298 1.17-3.14.38-.426.834-.767 1.338-1 .51-.237 1.06-.36 1.617-.36L6.632 6H7l7.932-2.895A2.363 2.363 0 0 1 18 5.36v9.28a2.36 2.36 0 0 1-3.069 2.25l.084.03L7 14.997H6v1.057Zm9.637-11.057a.415.415 0 0 0-.083.008L8 7.638v5.536l7.424 1.786.104.02c.035.01.072.02.109.02.2 0 .363-.16.363-.36V5.36c0-.2-.163-.363-.363-.363Zm-9.638 3h-.874a1.82 1.82 0 0 0-.625.111l-.15.063a2.128 2.128 0 0 0-.689.517c-.42.47-.661 1.123-.661 1.81 0 .34.06.678.176.992.114.308.28.585.485.816.4.447.925.691 1.464.691h.874v-5Z" clip-rule="evenodd"/><path d="M20 8.997h2a1 1 0 1 1 0 2h-2a1 1 0 1 1 0-2ZM20.707 14.293l1.414 1.414a1 1 0 0 1-1.414 1.414l-1.414-1.414a1 1 0 0 1 1.414-1.414Z"/></g></symbol><symbol id="icon-eds-i-card-medium" viewBox="0 0 24 24"><path d="M19.615 2c.315 0 .716.067 1.14.279.76.38 1.245 1.107 1.245 2.106v15.23c0 .315-.067.716-.279 1.14-.38.76-1.107 1.245-2.106 1.245H4.385a2.56 2.56 0 0 1-1.14-.279C2.485 21.341 2 20.614 2 19.615V4.385c0-.315.067-.716.279-1.14C2.659 2.485 3.386 2 4.385 2h15.23Zm0 2H4.385c-.213 0-.265.034-.317.14A.71.71 0 0 0 4 4.385v15.23c0 .213.034.265.14.317a.71.71 0 0 0 .245.068h15.23c.213 0 .265-.034.317-.14a.71.71 0 0 0 .068-.245V4.385c0-.213-.034-.265-.14-.317A.71.71 0 0 0 19.615 4ZM17 16a1 1 0 0 1 0 2H7a1 1 0 0 1 0-2h10Zm0-3a1 1 0 0 1 0 2H7a1 1 0 0 1 0-2h10Zm-.5-7A1.5 1.5 0 0 1 18 7.5v3a1.5 1.5 0 0 1-1.5 1.5h-9A1.5 1.5 0 0 1 6 10.5v-3A1.5 1.5 0 0 1 7.5 6h9ZM16 8H8v2h8V8Z"/></symbol><symbol id="icon-eds-i-cart-medium" viewBox="0 0 24 24"><path d="M5.76 1a1 1 0 0 1 .994.902L7.155 6h13.34c.18 0 .358.02.532.057l.174.045a2.5 2.5 0 0 1 1.693 3.103l-2.069 7.03c-.36 1.099-1.398 1.823-2.49 1.763H8.65c-1.272.015-2.352-.927-2.546-2.244L4.852 3H2a1 1 0 0 1-.993-.883L1 2a1 1 0 0 1 1-1h3.76Zm2.328 14.51a.555.555 0 0 0 .55.488l9.751.001a.533.533 0 0 0 .527-.357l2.059-7a.5.5 0 0 0-.48-.642H7.351l.737 7.51ZM18 19a2 2 0 1 1 0 4 2 2 0 0 1 0-4ZM8 19a2 2 0 1 1 0 4 2 2 0 0 1 0-4Z"/></symbol><symbol id="icon-eds-i-check-circle-medium" viewBox="0 0 24 24"><path d="M12 1c6.075 0 11 4.925 11 11s-4.925 11-11 11S1 18.075 1 12 5.925 1 12 1Zm0 2a9 9 0 1 0 0 18 9 9 0 0 0 0-18Zm5.125 4.72a1 1 0 0 1 .156 1.405l-6 7.5a1 1 0 0 1-1.421.143l-3-2.5a1 1 0 0 1 1.28-1.536l2.217 1.846 5.362-6.703a1 1 0 0 1 1.406-.156Z"/></symbol><symbol id="icon-eds-i-check-filled-medium" viewBox="0 0 24 24"><path d="M12 1c6.075 0 11 4.925 11 11s-4.925 11-11 11S1 18.075 1 12 5.925 1 12 1Zm5.125 6.72a1 1 0 0 0-1.406.155l-5.362 6.703-2.217-1.846a1 1 0 1 0-1.28 1.536l3 2.5a1 1 0 0 0 1.42-.143l6-7.5a1 1 0 0 0-.155-1.406Z"/></symbol><symbol id="icon-eds-i-chevron-down-medium" viewBox="0 0 24 24"><path d="M3.305 8.28a1 1 0 0 0-.024 1.415l7.495 7.762c.314.345.757.543 1.224.543.467 0 .91-.198 1.204-.522l7.515-7.783a1 1 0 1 0-1.438-1.39L12 15.845l-7.28-7.54A1 1 0 0 0 3.4 8.2l-.096.082Z"/></symbol><symbol id="icon-eds-i-chevron-down-small" viewBox="0 0 16 16"><path d="M13.692 5.278a1 1 0 0 1 .03 1.414L9.103 11.51a1.491 1.491 0 0 1-2.188.019L2.278 6.692a1 1 0 0 1 1.444-1.384L8 9.771l4.278-4.463a1 1 0 0 1 1.318-.111l.096.081Z"/></symbol><symbol id="icon-eds-i-chevron-left-medium" viewBox="0 0 24 24"><path d="M15.72 3.305a1 1 0 0 0-1.415-.024l-7.762 7.495A1.655 1.655 0 0 0 6 12c0 .467.198.91.522 1.204l7.783 7.515a1 1 0 1 0 1.39-1.438L8.155 12l7.54-7.28A1 1 0 0 0 15.8 3.4l-.082-.096Z"/></symbol><symbol id="icon-eds-i-chevron-left-small" viewBox="0 0 16 16"><path d="M10.722 2.308a1 1 0 0 0-1.414-.03L4.49 6.897a1.491 1.491 0 0 0-.019 2.188l4.838 4.637a1 1 0 1 0 1.384-1.444L6.229 8l4.463-4.278a1 1 0 0 0 .111-1.318l-.081-.096Z"/></symbol><symbol id="icon-eds-i-chevron-right-medium" viewBox="0 0 24 24"><path d="M8.28 3.305a1 1 0 0 1 1.415-.024l7.762 7.495c.345.314.543.757.543 1.224 0 .467-.198.91-.522 1.204l-7.783 7.515a1 1 0 1 1-1.39-1.438L15.845 12l-7.54-7.28A1 1 0 0 1 8.2 3.4l.082-.096Z"/></symbol><symbol id="icon-eds-i-chevron-right-small" viewBox="0 0 16 16"><path d="M5.278 2.308a1 1 0 0 1 1.414-.03l4.819 4.619a1.491 1.491 0 0 1 .019 2.188l-4.838 4.637a1 1 0 1 1-1.384-1.444L9.771 8 5.308 3.722a1 1 0 0 1-.111-1.318l.081-.096Z"/></symbol><symbol id="icon-eds-i-chevron-up-medium" viewBox="0 0 24 24"><path d="M20.695 15.72a1 1 0 0 0 .024-1.415l-7.495-7.762A1.655 1.655 0 0 0 12 6c-.467 0-.91.198-1.204.522l-7.515 7.783a1 1 0 1 0 1.438 1.39L12 8.155l7.28 7.54a1 1 0 0 0 1.319.106l.096-.082Z"/></symbol><symbol id="icon-eds-i-chevron-up-small" viewBox="0 0 16 16"><path d="M13.692 10.722a1 1 0 0 0 .03-1.414L9.103 4.49a1.491 1.491 0 0 0-2.188-.019L2.278 9.308a1 1 0 0 0 1.444 1.384L8 6.229l4.278 4.463a1 1 0 0 0 1.318.111l.096-.081Z"/></symbol><symbol id="icon-eds-i-citations-medium" viewBox="0 0 24 24"><path d="M15.59 1a1 1 0 0 1 .706.291l5.41 5.385a1 1 0 0 1 .294.709v13.077c0 .674-.269 1.32-.747 1.796a2.549 2.549 0 0 1-1.798.742h-5.843a1 1 0 1 1 0-2h5.843a.549.549 0 0 0 .387-.16.535.535 0 0 0 .158-.378V7.8L15.178 3H5.545a.543.543 0 0 0-.538.451L5 3.538v8.607a1 1 0 0 1-2 0V3.538A2.542 2.542 0 0 1 5.545 1h10.046ZM5.483 14.35c.197.26.17.62-.049.848l-.095.083-.016.011c-.36.24-.628.45-.804.634-.393.409-.59.93-.59 1.562.077-.019.192-.028.345-.028.442 0 .84.158 1.195.474.355.316.532.716.532 1.2 0 .501-.173.9-.518 1.198-.345.298-.767.446-1.266.446-.672 0-1.209-.195-1.612-.585-.403-.39-.604-.976-.604-1.757 0-.744.11-1.39.33-1.938.222-.549.49-1.009.807-1.38a4.28 4.28 0 0 1 .992-.88c.07-.043.148-.087.232-.133a.881.881 0 0 1 1.121.245Zm5 0c.197.26.17.62-.049.848l-.095.083-.016.011c-.36.24-.628.45-.804.634-.393.409-.59.93-.59 1.562.077-.019.192-.028.345-.028.442 0 .84.158 1.195.474.355.316.532.716.532 1.2 0 .501-.173.9-.518 1.198-.345.298-.767.446-1.266.446-.672 0-1.209-.195-1.612-.585-.403-.39-.604-.976-.604-1.757 0-.744.11-1.39.33-1.938.222-.549.49-1.009.807-1.38a4.28 4.28 0 0 1 .992-.88c.07-.043.148-.087.232-.133a.881.881 0 0 1 1.121.245Z"/></symbol><symbol id="icon-eds-i-clipboard-check-medium" viewBox="0 0 24 24"><path d="M14.4 1c1.238 0 2.274.865 2.536 2.024L18.5 3C19.886 3 21 4.14 21 5.535v14.93C21 21.86 19.886 23 18.5 23h-13C4.114 23 3 21.86 3 20.465V5.535C3 4.14 4.114 3 5.5 3h1.57c.27-1.147 1.3-2 2.53-2h4.8Zm4.115 4-1.59.024A2.601 2.601 0 0 1 14.4 7H9.6c-1.23 0-2.26-.853-2.53-2H5.5c-.27 0-.5.234-.5.535v14.93c0 .3.23.535.5.535h13c.27 0 .5-.234.5-.535V5.535c0-.3-.23-.535-.485-.535Zm-1.909 4.205a1 1 0 0 1 .19 1.401l-5.334 7a1 1 0 0 1-1.344.23l-2.667-1.75a1 1 0 1 1 1.098-1.672l1.887 1.238 4.769-6.258a1 1 0 0 1 1.401-.19ZM14.4 3H9.6a.6.6 0 0 0-.6.6v.8a.6.6 0 0 0 .6.6h4.8a.6.6 0 0 0 .6-.6v-.8a.6.6 0 0 0-.6-.6Z"/></symbol><symbol id="icon-eds-i-clipboard-report-medium" viewBox="0 0 24 24"><path d="M14.4 1c1.238 0 2.274.865 2.536 2.024L18.5 3C19.886 3 21 4.14 21 5.535v14.93C21 21.86 19.886 23 18.5 23h-13C4.114 23 3 21.86 3 20.465V5.535C3 4.14 4.114 3 5.5 3h1.57c.27-1.147 1.3-2 2.53-2h4.8Zm4.115 4-1.59.024A2.601 2.601 0 0 1 14.4 7H9.6c-1.23 0-2.26-.853-2.53-2H5.5c-.27 0-.5.234-.5.535v14.93c0 .3.23.535.5.535h13c.27 0 .5-.234.5-.535V5.535c0-.3-.23-.535-.485-.535Zm-2.658 10.929a1 1 0 0 1 0 2H8a1 1 0 0 1 0-2h7.857Zm0-3.929a1 1 0 0 1 0 2H8a1 1 0 0 1 0-2h7.857ZM14.4 3H9.6a.6.6 0 0 0-.6.6v.8a.6.6 0 0 0 .6.6h4.8a.6.6 0 0 0 .6-.6v-.8a.6.6 0 0 0-.6-.6Z"/></symbol><symbol id="icon-eds-i-close-medium" viewBox="0 0 24 24"><path d="M12 1c6.075 0 11 4.925 11 11s-4.925 11-11 11S1 18.075 1 12 5.925 1 12 1Zm0 2a9 9 0 1 0 0 18 9 9 0 0 0 0-18ZM8.707 7.293 12 10.585l3.293-3.292a1 1 0 0 1 1.414 1.414L13.415 12l3.292 3.293a1 1 0 0 1-1.414 1.414L12 13.415l-3.293 3.292a1 1 0 1 1-1.414-1.414L10.585 12 7.293 8.707a1 1 0 0 1 1.414-1.414Z"/></symbol><symbol id="icon-eds-i-cloud-upload-medium" viewBox="0 0 24 24"><path d="m12.852 10.011.028-.004L13 10l.075.003.126.017.086.022.136.052.098.052.104.074.082.073 3 3a1 1 0 0 1 0 1.414l-.094.083a1 1 0 0 1-1.32-.083L14 13.416V20a1 1 0 0 1-2 0v-6.586l-1.293 1.293a1 1 0 0 1-1.32.083l-.094-.083a1 1 0 0 1 0-1.414l3-3 .112-.097.11-.071.114-.054.105-.035.118-.025Zm.587-7.962c3.065.362 5.497 2.662 5.992 5.562l.013.085.207.073c2.117.782 3.496 2.845 3.337 5.097l-.022.226c-.297 2.561-2.503 4.491-5.124 4.502a1 1 0 1 1-.009-2c1.619-.007 2.967-1.186 3.147-2.733.179-1.542-.86-2.979-2.487-3.353-.512-.149-.894-.579-.981-1.165-.21-2.237-2-4.035-4.308-4.308-2.31-.273-4.497 1.06-5.25 3.19l-.049.113c-.234.468-.718.756-1.176.743-1.418.057-2.689.857-3.32 2.084a3.668 3.668 0 0 0 .262 3.798c.796 1.136 2.169 1.764 3.583 1.635a1 1 0 1 1 .182 1.992c-2.125.194-4.193-.753-5.403-2.48a5.668 5.668 0 0 1-.403-5.86c.85-1.652 2.449-2.79 4.323-3.092l.287-.039.013-.028c1.207-2.741 4.125-4.404 7.186-4.042Z"/></symbol><symbol id="icon-eds-i-collection-medium" viewBox="0 0 24 24"><path d="M21 7a1 1 0 0 1 1 1v12.5a2.5 2.5 0 0 1-2.5 2.5H8a1 1 0 0 1 0-2h11.5a.5.5 0 0 0 .5-.5V8a1 1 0 0 1 1-1Zm-5.5-5A2.5 2.5 0 0 1 18 4.5v12a2.5 2.5 0 0 1-2.5 2.5h-11A2.5 2.5 0 0 1 2 16.5v-12A2.5 2.5 0 0 1 4.5 2h11Zm0 2h-11a.5.5 0 0 0-.5.5v12a.5.5 0 0 0 .5.5h11a.5.5 0 0 0 .5-.5v-12a.5.5 0 0 0-.5-.5ZM13 13a1 1 0 0 1 0 2H7a1 1 0 0 1 0-2h6Zm0-3.5a1 1 0 0 1 0 2H7a1 1 0 0 1 0-2h6ZM13 6a1 1 0 0 1 0 2H7a1 1 0 1 1 0-2h6Z"/></symbol><symbol id="icon-eds-i-conference-series-medium" viewBox="0 0 24 24"><path fill-rule="evenodd" d="M4.5 2A2.5 2.5 0 0 0 2 4.5v11A2.5 2.5 0 0 0 4.5 18h2.37l-2.534 2.253a1 1 0 0 0 1.328 1.494L9.88 18H11v3a1 1 0 1 0 2 0v-3h1.12l4.216 3.747a1 1 0 0 0 1.328-1.494L17.13 18h2.37a2.5 2.5 0 0 0 2.5-2.5v-11A2.5 2.5 0 0 0 19.5 2h-15ZM20 6V4.5a.5.5 0 0 0-.5-.5h-15a.5.5 0 0 0-.5.5V6h16ZM4 8v7.5a.5.5 0 0 0 .5.5h15a.5.5 0 0 0 .5-.5V8H4Z" clip-rule="evenodd"/></symbol><symbol id="icon-eds-i-delivery-medium" viewBox="0 0 24 24"><path d="M8.51 20.598a3.037 3.037 0 0 1-3.02 0A2.968 2.968 0 0 1 4.161 19L3.5 19A2.5 2.5 0 0 1 1 16.5v-11A2.5 2.5 0 0 1 3.5 3h10a2.5 2.5 0 0 1 2.45 2.004L16 5h2.527c.976 0 1.855.585 2.27 1.49l2.112 4.62a1 1 0 0 1 .091.416v4.856C23 17.814 21.889 19 20.484 19h-.523a1.01 1.01 0 0 1-.121-.007 2.96 2.96 0 0 1-1.33 1.605 3.037 3.037 0 0 1-3.02 0A2.968 2.968 0 0 1 14.161 19H9.838a2.968 2.968 0 0 1-1.327 1.597Zm-2.024-3.462a.955.955 0 0 0-.481.73L5.999 18l.001.022a.944.944 0 0 0 .388.777l.098.065c.316.181.712.181 1.028 0A.97.97 0 0 0 8 17.978a.95.95 0 0 0-.486-.842 1.037 1.037 0 0 0-1.028 0Zm10 0a.955.955 0 0 0-.481.73l-.005.156a.944.944 0 0 0 .388.777l.098.065c.316.181.712.181 1.028 0a.97.97 0 0 0 .486-.886.95.95 0 0 0-.486-.842 1.037 1.037 0 0 0-1.028 0ZM21 12h-5v3.17a3.038 3.038 0 0 1 2.51.232 2.993 2.993 0 0 1 1.277 1.45l.058.155.058-.005.581-.002c.27 0 .516-.263.516-.618V12Zm-7.5-7h-10a.5.5 0 0 0-.5.5v11a.5.5 0 0 0 .5.5h.662a2.964 2.964 0 0 1 1.155-1.491l.172-.107a3.037 3.037 0 0 1 3.022 0A2.987 2.987 0 0 1 9.843 17H13.5a.5.5 0 0 0 .5-.5v-11a.5.5 0 0 0-.5-.5Zm5.027 2H16v3h4.203l-1.224-2.677a.532.532 0 0 0-.375-.316L18.527 7Z"/></symbol><symbol id="icon-eds-i-download-medium" viewBox="0 0 24 24"><path d="M22 18.5a3.5 3.5 0 0 1-3.5 3.5h-13A3.5 3.5 0 0 1 2 18.5V18a1 1 0 0 1 2 0v.5A1.5 1.5 0 0 0 5.5 20h13a1.5 1.5 0 0 0 1.5-1.5V18a1 1 0 0 1 2 0v.5Zm-3.293-7.793-6 6-.063.059-.093.069-.081.048-.105.049-.104.034-.056.013-.118.017L12 17l-.076-.003-.122-.017-.113-.03-.085-.032-.063-.03-.098-.058-.06-.043-.05-.043-6.04-6.037a1 1 0 0 1 1.414-1.414l4.294 4.29L11 3a1 1 0 0 1 2 0l.001 10.585 4.292-4.292a1 1 0 0 1 1.32-.083l.094.083a1 1 0 0 1 0 1.414Z"/></symbol><symbol id="icon-eds-i-edit-medium" viewBox="0 0 24 24"><path d="M17.149 2a2.38 2.38 0 0 1 1.699.711l2.446 2.46a2.384 2.384 0 0 1 .005 3.38L10.01 19.906a1 1 0 0 1-.434.257l-6.3 1.8a1 1 0 0 1-1.237-1.237l1.8-6.3a1 1 0 0 1 .257-.434L15.443 2.718A2.385 2.385 0 0 1 17.15 2Zm-3.874 5.689-7.586 7.536-1.234 4.319 4.318-1.234 7.54-7.582-3.038-3.039ZM17.149 4a.395.395 0 0 0-.286.126L14.695 6.28l3.029 3.029 2.162-2.173a.384.384 0 0 0 .106-.197L20 6.864c0-.103-.04-.2-.119-.278l-2.457-2.47A.385.385 0 0 0 17.149 4Z"/></symbol><symbol id="icon-eds-i-education-medium" viewBox="0 0 24 24"><path fill-rule="evenodd" d="M12.41 2.088a1 1 0 0 0-.82 0l-10 4.5a1 1 0 0 0 0 1.824L3 9.047v7.124A3.001 3.001 0 0 0 4 22a3 3 0 0 0 1-5.83V9.948l1 .45V14.5a1 1 0 0 0 .087.408L7 14.5c-.913.408-.912.41-.912.41l.001.003.003.006.007.015a1.988 1.988 0 0 0 .083.16c.054.097.131.225.236.373.21.297.53.68.993 1.057C8.351 17.292 9.824 18 12 18c2.176 0 3.65-.707 4.589-1.476.463-.378.783-.76.993-1.057a4.162 4.162 0 0 0 .319-.533l.007-.015.003-.006v-.003h.002s0-.002-.913-.41l.913.408A1 1 0 0 0 18 14.5v-4.103l4.41-1.985a1 1 0 0 0 0-1.824l-10-4.5ZM16 11.297l-3.59 1.615a1 1 0 0 1-.82 0L8 11.297v2.94a3.388 3.388 0 0 0 .677.739C9.267 15.457 10.294 16 12 16s2.734-.543 3.323-1.024a3.388 3.388 0 0 0 .677-.739v-2.94ZM4.437 7.5 12 4.097 19.563 7.5 12 10.903 4.437 7.5ZM3 19a1 1 0 1 1 2 0 1 1 0 0 1-2 0Z" clip-rule="evenodd"/></symbol><symbol id="icon-eds-i-error-diamond-medium" viewBox="0 0 24 24"><path d="M12.002 1c.702 0 1.375.279 1.871.775l8.35 8.353a2.646 2.646 0 0 1 .001 3.744l-8.353 8.353a2.646 2.646 0 0 1-3.742 0l-8.353-8.353a2.646 2.646 0 0 1 0-3.744l8.353-8.353.156-.142c.424-.362.952-.58 1.507-.625l.21-.008Zm0 2a.646.646 0 0 0-.38.123l-.093.08-8.34 8.34a.646.646 0 0 0-.18.355L3 12c0 .171.068.336.19.457l8.353 8.354a.646.646 0 0 0 .914 0l8.354-8.354a.646.646 0 0 0-.001-.914l-8.351-8.354A.646.646 0 0 0 12.002 3ZM12 14.5a1.5 1.5 0 0 1 .144 2.993L12 17.5a1.5 1.5 0 0 1 0-3ZM12 6a1 1 0 0 1 1 1v5a1 1 0 0 1-2 0V7a1 1 0 0 1 1-1Z"/></symbol><symbol id="icon-eds-i-error-filled-medium" viewBox="0 0 24 24"><path d="M12.002 1c.702 0 1.375.279 1.871.775l8.35 8.353a2.646 2.646 0 0 1 .001 3.744l-8.353 8.353a2.646 2.646 0 0 1-3.742 0l-8.353-8.353a2.646 2.646 0 0 1 0-3.744l8.353-8.353.156-.142c.424-.362.952-.58 1.507-.625l.21-.008ZM12 14.5a1.5 1.5 0 0 0 0 3l.144-.007A1.5 1.5 0 0 0 12 14.5ZM12 6a1 1 0 0 0-1 1v5a1 1 0 0 0 2 0V7a1 1 0 0 0-1-1Z"/></symbol><symbol id="icon-eds-i-external-link-medium" viewBox="0 0 24 24"><path d="M9 2a1 1 0 1 1 0 2H4.6c-.371 0-.6.209-.6.5v15c0 .291.229.5.6.5h14.8c.371 0 .6-.209.6-.5V15a1 1 0 0 1 2 0v4.5c0 1.438-1.162 2.5-2.6 2.5H4.6C3.162 22 2 20.938 2 19.5v-15C2 3.062 3.162 2 4.6 2H9Zm6 0h6l.075.003.126.017.111.03.111.044.098.052.096.067.09.08c.036.035.068.073.097.112l.071.11.054.114.035.105.03.148L22 3v6a1 1 0 0 1-2 0V5.414l-6.693 6.693a1 1 0 0 1-1.414-1.414L18.584 4H15a1 1 0 0 1-.993-.883L14 3a1 1 0 0 1 1-1Z"/></symbol><symbol id="icon-eds-i-external-link-small" viewBox="0 0 16 16"><path d="M5 1a1 1 0 1 1 0 2l-2-.001V13L13 13v-2a1 1 0 0 1 2 0v2c0 1.15-.93 2-2.067 2H3.067C1.93 15 1 14.15 1 13V3c0-1.15.93-2 2.067-2H5Zm4 0h5l.075.003.126.017.111.03.111.044.098.052.096.067.09.08.044.047.073.093.051.083.054.113.035.105.03.148L15 2v5a1 1 0 0 1-2 0V4.414L9.107 8.307a1 1 0 0 1-1.414-1.414L11.584 3H9a1 1 0 0 1-.993-.883L8 2a1 1 0 0 1 1-1Z"/></symbol><symbol id="icon-eds-i-file-download-medium" viewBox="0 0 24 24"><path d="M14.5 1a1 1 0 0 1 .707.293l5.5 5.5A1 1 0 0 1 21 7.5v12.962A2.542 2.542 0 0 1 18.455 23H5.545A2.542 2.542 0 0 1 3 20.462V3.538A2.542 2.542 0 0 1 5.545 1H14.5Zm-.415 2h-8.54A.542.542 0 0 0 5 3.538v16.924c0 .296.243.538.545.538h12.91a.542.542 0 0 0 .545-.538V7.915L14.085 3ZM12 7a1 1 0 0 1 1 1v6.585l2.293-2.292a1 1 0 0 1 1.32-.083l.094.083a1 1 0 0 1 0 1.414l-4 4a1.008 1.008 0 0 1-.112.097l-.11.071-.114.054-.105.035-.149.03L12 18l-.075-.003-.126-.017-.111-.03-.111-.044-.098-.052-.096-.067-.09-.08-4-4a1 1 0 0 1 1.414-1.414L11 14.585V8a1 1 0 0 1 1-1Z"/></symbol><symbol id="icon-eds-i-file-report-medium" viewBox="0 0 24 24"><path d="M14.5 1a1 1 0 0 1 .707.293l5.5 5.5A1 1 0 0 1 21 7.5v12.962c0 .674-.269 1.32-.747 1.796a2.549 2.549 0 0 1-1.798.742H5.545c-.674 0-1.32-.267-1.798-.742A2.535 2.535 0 0 1 3 20.462V3.538A2.542 2.542 0 0 1 5.545 1H14.5Zm-.415 2h-8.54A.542.542 0 0 0 5 3.538v16.924c0 .142.057.278.158.379.102.102.242.159.387.159h12.91a.549.549 0 0 0 .387-.16.535.535 0 0 0 .158-.378V7.915L14.085 3ZM16 17a1 1 0 0 1 0 2H8a1 1 0 0 1 0-2h8Zm0-3a1 1 0 0 1 0 2H8a1 1 0 0 1 0-2h8Zm-4.793-6.207L13 9.585l1.793-1.792a1 1 0 0 1 1.32-.083l.094.083a1 1 0 0 1 0 1.414l-2.5 2.5a1 1 0 0 1-1.414 0L10.5 9.915l-1.793 1.792a1 1 0 0 1-1.32.083l-.094-.083a1 1 0 0 1 0-1.414l2.5-2.5a1 1 0 0 1 1.414 0Z"/></symbol><symbol id="icon-eds-i-file-text-medium" viewBox="0 0 24 24"><path d="M14.5 1a1 1 0 0 1 .707.293l5.5 5.5A1 1 0 0 1 21 7.5v12.962A2.542 2.542 0 0 1 18.455 23H5.545A2.542 2.542 0 0 1 3 20.462V3.538A2.542 2.542 0 0 1 5.545 1H14.5Zm-.415 2h-8.54A.542.542 0 0 0 5 3.538v16.924c0 .296.243.538.545.538h12.91a.542.542 0 0 0 .545-.538V7.915L14.085 3ZM16 15a1 1 0 0 1 0 2H8a1 1 0 0 1 0-2h8Zm0-4a1 1 0 0 1 0 2H8a1 1 0 0 1 0-2h8Zm-5-4a1 1 0 0 1 0 2H8a1 1 0 1 1 0-2h3Z"/></symbol><symbol id="icon-eds-i-file-upload-medium" viewBox="0 0 24 24"><path d="M14.5 1a1 1 0 0 1 .707.293l5.5 5.5A1 1 0 0 1 21 7.5v12.962A2.542 2.542 0 0 1 18.455 23H5.545A2.542 2.542 0 0 1 3 20.462V3.538A2.542 2.542 0 0 1 5.545 1H14.5Zm-.415 2h-8.54A.542.542 0 0 0 5 3.538v16.924c0 .296.243.538.545.538h12.91a.542.542 0 0 0 .545-.538V7.915L14.085 3Zm-2.233 4.011.058-.007L12 7l.075.003.126.017.111.03.111.044.098.052.104.074.082.073 4 4a1 1 0 0 1 0 1.414l-.094.083a1 1 0 0 1-1.32-.083L13 10.415V17a1 1 0 0 1-2 0v-6.585l-2.293 2.292a1 1 0 0 1-1.32.083l-.094-.083a1 1 0 0 1 0-1.414l4-4 .112-.097.11-.071.114-.054.105-.035.118-.025Z"/></symbol><symbol id="icon-eds-i-filter-medium" viewBox="0 0 24 24"><path d="M21 2a1 1 0 0 1 .82 1.573L15 13.314V18a1 1 0 0 1-.31.724l-.09.076-4 3A1 1 0 0 1 9 21v-7.684L2.18 3.573a1 1 0 0 1 .707-1.567L3 2h18Zm-1.921 2H4.92l5.9 8.427a1 1 0 0 1 .172.45L11 13v6l2-1.5V13a1 1 0 0 1 .117-.469l.064-.104L19.079 4Z"/></symbol><symbol id="icon-eds-i-funding-medium" viewBox="0 0 24 24"><path fill-rule="evenodd" d="M23 8A7 7 0 1 0 9 8a7 7 0 0 0 14 0ZM9.006 12.225A4.07 4.07 0 0 0 6.12 11.02H2a.979.979 0 1 0 0 1.958h4.12c.558 0 1.094.222 1.489.617l2.207 2.288c.27.27.27.687.012.944a.656.656 0 0 1-.928 0L7.744 15.67a.98.98 0 0 0-1.386 1.384l1.157 1.158c.535.536 1.244.791 1.946.765l.041.002h6.922c.874 0 1.597.748 1.597 1.688 0 .203-.146.354-.309.354H7.755c-.487 0-.96-.178-1.339-.504L2.64 17.259a.979.979 0 0 0-1.28 1.482L5.137 22c.733.631 1.66.979 2.618.979h9.957c1.26 0 2.267-1.043 2.267-2.312 0-2.006-1.584-3.646-3.555-3.646h-4.529a2.617 2.617 0 0 0-.681-2.509l-2.208-2.287ZM16 3a5 5 0 1 0 0 10 5 5 0 0 0 0-10Zm.979 3.5a.979.979 0 1 0-1.958 0v3a.979.979 0 1 0 1.958 0v-3Z" clip-rule="evenodd"/></symbol><symbol id="icon-eds-i-hashtag-medium" viewBox="0 0 24 24"><path d="M12 1c6.075 0 11 4.925 11 11s-4.925 11-11 11S1 18.075 1 12 5.925 1 12 1Zm0 2a9 9 0 1 0 0 18 9 9 0 0 0 0-18ZM9.52 18.189a1 1 0 1 1-1.964-.378l.437-2.274H6a1 1 0 1 1 0-2h2.378l.592-3.076H6a1 1 0 0 1 0-2h3.354l.51-2.65a1 1 0 1 1 1.964.378l-.437 2.272h3.04l.51-2.65a1 1 0 1 1 1.964.378l-.438 2.272H18a1 1 0 0 1 0 2h-1.917l-.592 3.076H18a1 1 0 0 1 0 2h-2.893l-.51 2.652a1 1 0 1 1-1.964-.378l.437-2.274h-3.04l-.51 2.652Zm.895-4.652h3.04l.591-3.076h-3.04l-.591 3.076Z"/></symbol><symbol id="icon-eds-i-home-medium" viewBox="0 0 24 24"><path d="M5 22a1 1 0 0 1-1-1v-8.586l-1.293 1.293a1 1 0 0 1-1.32.083l-.094-.083a1 1 0 0 1 0-1.414l10-10a1 1 0 0 1 1.414 0l10 10a1 1 0 0 1-1.414 1.414L20 12.415V21a1 1 0 0 1-1 1H5Zm7-17.585-6 5.999V20h5v-4a1 1 0 0 1 2 0v4h5v-9.585l-6-6Z"/></symbol><symbol id="icon-eds-i-image-medium" viewBox="0 0 24 24"><path d="M19.615 2A2.385 2.385 0 0 1 22 4.385v15.23A2.385 2.385 0 0 1 19.615 22H4.385A2.385 2.385 0 0 1 2 19.615V4.385A2.385 2.385 0 0 1 4.385 2h15.23Zm0 2H4.385A.385.385 0 0 0 4 4.385v15.23c0 .213.172.385.385.385h1.244l10.228-8.76a1 1 0 0 1 1.254-.037L20 13.392V4.385A.385.385 0 0 0 19.615 4Zm-3.07 9.283L8.703 20h10.912a.385.385 0 0 0 .385-.385v-3.713l-3.455-2.619ZM9.5 6a3.5 3.5 0 1 1 0 7 3.5 3.5 0 0 1 0-7Zm0 2a1.5 1.5 0 1 0 0 3 1.5 1.5 0 0 0 0-3Z"/></symbol><symbol id="icon-eds-i-impact-factor-medium" viewBox="0 0 24 24"><path d="M16.49 2.672c.74.694.986 1.765.632 2.712l-.04.1-1.549 3.54h1.477a2.496 2.496 0 0 1 2.485 2.34l.005.163c0 .618-.23 1.21-.642 1.675l-7.147 7.961a2.48 2.48 0 0 1-3.554.165 2.512 2.512 0 0 1-.633-2.712l.042-.103L9.108 15H7.46c-1.393 0-2.379-1.11-2.455-2.369L5 12.473c0-.593.142-1.145.628-1.692l7.307-7.944a2.48 2.48 0 0 1 3.555-.165ZM14.43 4.164l-7.33 7.97c-.083.093-.101.214-.101.34 0 .277.19.526.46.526h4.163l.097-.009c.015 0 .03.003.046.009.181.078.264.32.186.5l-2.554 5.817a.512.512 0 0 0 .127.552.48.48 0 0 0 .69-.033l7.155-7.97a.513.513 0 0 0 .13-.34.497.497 0 0 0-.49-.502h-3.988a.355.355 0 0 1-.328-.497l2.555-5.844a.512.512 0 0 0-.127-.552.48.48 0 0 0-.69.033Z"/></symbol><symbol id="icon-eds-i-info-circle-medium" viewBox="0 0 24 24"><path d="M12 1c6.075 0 11 4.925 11 11s-4.925 11-11 11S1 18.075 1 12 5.925 1 12 1Zm0 2a9 9 0 1 0 0 18 9 9 0 0 0 0-18Zm0 7a1 1 0 0 1 1 1v5h1.5a1 1 0 0 1 0 2h-5a1 1 0 0 1 0-2H11v-4h-.5a1 1 0 0 1-.993-.883L9.5 11a1 1 0 0 1 1-1H12Zm0-4.5a1.5 1.5 0 0 1 .144 2.993L12 8.5a1.5 1.5 0 0 1 0-3Z"/></symbol><symbol id="icon-eds-i-info-filled-medium" viewBox="0 0 24 24"><path d="M12 1c6.075 0 11 4.925 11 11s-4.925 11-11 11S1 18.075 1 12 5.925 1 12 1Zm0 9h-1.5a1 1 0 0 0-1 1l.007.117A1 1 0 0 0 10.5 12h.5v4H9.5a1 1 0 0 0 0 2h5a1 1 0 0 0 0-2H13v-5a1 1 0 0 0-1-1Zm0-4.5a1.5 1.5 0 0 0 0 3l.144-.007A1.5 1.5 0 0 0 12 5.5Z"/></symbol><symbol id="icon-eds-i-journal-medium" viewBox="0 0 24 24"><path d="M18.5 1A2.5 2.5 0 0 1 21 3.5v14a2.5 2.5 0 0 1-2.5 2.5h-13a.5.5 0 1 0 0 1H20a1 1 0 0 1 0 2H5.5A2.5 2.5 0 0 1 3 20.5v-17A2.5 2.5 0 0 1 5.5 1h13ZM7 3H5.5a.5.5 0 0 0-.5.5v14.549l.016-.002c.104-.02.211-.035.32-.042L5.5 18H7V3Zm11.5 0H9v15h9.5a.5.5 0 0 0 .5-.5v-14a.5.5 0 0 0-.5-.5ZM16 5a1 1 0 0 1 1 1v4a1 1 0 0 1-1 1h-5a1 1 0 0 1-1-1V6a1 1 0 0 1 1-1h5Zm-1 2h-3v2h3V7Z"/></symbol><symbol id="icon-eds-i-mail-medium" viewBox="0 0 24 24"><path d="M20.462 3C21.875 3 23 4.184 23 5.619v12.762C23 19.816 21.875 21 20.462 21H3.538C2.125 21 1 19.816 1 18.381V5.619C1 4.184 2.125 3 3.538 3h16.924ZM21 8.158l-7.378 6.258a2.549 2.549 0 0 1-3.253-.008L3 8.16v10.222c0 .353.253.619.538.619h16.924c.285 0 .538-.266.538-.619V8.158ZM20.462 5H3.538c-.264 0-.5.228-.534.542l8.65 7.334c.2.165.492.165.684.007l8.656-7.342-.001-.025c-.044-.3-.274-.516-.531-.516Z"/></symbol><symbol id="icon-eds-i-mail-send-medium" viewBox="0 0 24 24"><path d="M20.444 5a2.562 2.562 0 0 1 2.548 2.37l.007.078.001.123v7.858A2.564 2.564 0 0 1 20.444 18H9.556A2.564 2.564 0 0 1 7 15.429l.001-7.977.007-.082A2.561 2.561 0 0 1 9.556 5h10.888ZM21 9.331l-5.46 3.51a1 1 0 0 1-1.08 0L9 9.332v6.097c0 .317.251.571.556.571h10.888a.564.564 0 0 0 .556-.571V9.33ZM20.444 7H9.556a.543.543 0 0 0-.32.105l5.763 3.706 5.766-3.706a.543.543 0 0 0-.32-.105ZM4.308 5a1 1 0 1 1 0 2H2a1 1 0 1 1 0-2h2.308Zm0 5.5a1 1 0 0 1 0 2H2a1 1 0 0 1 0-2h2.308Zm0 5.5a1 1 0 0 1 0 2H2a1 1 0 0 1 0-2h2.308Z"/></symbol><symbol id="icon-eds-i-mentions-medium" viewBox="0 0 24 24"><path d="m9.452 1.293 5.92 5.92 2.92-2.92a1 1 0 0 1 1.415 1.414l-2.92 2.92 5.92 5.92a1 1 0 0 1 0 1.415 10.371 10.371 0 0 1-10.378 2.584l.652 3.258A1 1 0 0 1 12 23H2a1 1 0 0 1-.874-1.486l4.789-8.62C4.194 9.074 4.9 4.43 8.038 1.292a1 1 0 0 1 1.414 0Zm-2.355 13.59L3.699 21h7.081l-.689-3.442a10.392 10.392 0 0 1-2.775-2.396l-.22-.28Zm1.69-11.427-.07.09a8.374 8.374 0 0 0 11.737 11.737l.089-.071L8.787 3.456Z"/></symbol><symbol id="icon-eds-i-menu-medium" viewBox="0 0 24 24"><path d="M21 4a1 1 0 0 1 0 2H3a1 1 0 1 1 0-2h18Zm-4 7a1 1 0 0 1 0 2H3a1 1 0 0 1 0-2h14Zm4 7a1 1 0 0 1 0 2H3a1 1 0 0 1 0-2h18Z"/></symbol><symbol id="icon-eds-i-metrics-medium" viewBox="0 0 24 24"><path d="M3 22a1 1 0 0 1-1-1V3a1 1 0 0 1 1-1h6a1 1 0 0 1 1 1v7h4V8a1 1 0 0 1 1-1h6a1 1 0 0 1 1 1v13a1 1 0 0 1-.883.993L21 22H3Zm17-2V9h-4v11h4Zm-6-8h-4v8h4v-8ZM8 4H4v16h4V4Z"/></symbol><symbol id="icon-eds-i-news-medium" viewBox="0 0 24 24"><path d="M17.384 3c.975 0 1.77.787 1.77 1.762v13.333c0 .462.354.846.815.899l.107.006.109-.006a.915.915 0 0 0 .809-.794l.006-.105V8.19a1 1 0 0 1 2 0v9.905A2.914 2.914 0 0 1 20.077 21H3.538a2.547 2.547 0 0 1-1.644-.601l-.147-.135A2.516 2.516 0 0 1 1 18.476V4.762C1 3.787 1.794 3 2.77 3h14.614Zm-.231 2H3v13.476c0 .11.035.216.1.304l.054.063c.101.1.24.157.384.157l13.761-.001-.026-.078a2.88 2.88 0 0 1-.115-.655l-.004-.17L17.153 5ZM14 15.021a.979.979 0 1 1 0 1.958H6a.979.979 0 1 1 0-1.958h8Zm0-8c.54 0 .979.438.979.979v4c0 .54-.438.979-.979.979H6A.979.979 0 0 1 5.021 12V8c0-.54.438-.979.979-.979h8Zm-.98 1.958H6.979v2.041h6.041V8.979Z"/></symbol><symbol id="icon-eds-i-newsletter-medium" viewBox="0 0 24 24"><path d="M21 10a1 1 0 0 1 1 1v9.5a2.5 2.5 0 0 1-2.5 2.5h-15A2.5 2.5 0 0 1 2 20.5V11a1 1 0 0 1 2 0v.439l8 4.888 8-4.889V11a1 1 0 0 1 1-1Zm-1 3.783-7.479 4.57a1 1 0 0 1-1.042 0l-7.48-4.57V20.5a.5.5 0 0 0 .501.5h15a.5.5 0 0 0 .5-.5v-6.717ZM15 9a1 1 0 0 1 0 2H9a1 1 0 0 1 0-2h6Zm2.5-8A2.5 2.5 0 0 1 20 3.5V9a1 1 0 0 1-2 0V3.5a.5.5 0 0 0-.5-.5h-11a.5.5 0 0 0-.5.5V9a1 1 0 1 1-2 0V3.5A2.5 2.5 0 0 1 6.5 1h11ZM15 5a1 1 0 0 1 0 2H9a1 1 0 1 1 0-2h6Z"/></symbol><symbol id="icon-eds-i-notifcation-medium" viewBox="0 0 24 24"><path d="M14 20a1 1 0 0 1 0 2h-4a1 1 0 0 1 0-2h4ZM3 18l-.133-.007c-1.156-.124-1.156-1.862 0-1.986l.3-.012C4.32 15.923 5 15.107 5 14V9.5C5 5.368 8.014 2 12 2s7 3.368 7 7.5V14c0 1.107.68 1.923 1.832 1.995l.301.012c1.156.124 1.156 1.862 0 1.986L21 18H3Zm9-14C9.17 4 7 6.426 7 9.5V14c0 .671-.146 1.303-.416 1.858L6.51 16h10.979l-.073-.142a4.192 4.192 0 0 1-.412-1.658L17 14V9.5C17 6.426 14.83 4 12 4Z"/></symbol><symbol id="icon-eds-i-publish-medium" viewBox="0 0 24 24"><g><path d="M16.296 1.291A1 1 0 0 0 15.591 1H5.545A2.542 2.542 0 0 0 3 3.538V13a1 1 0 1 0 2 0V3.538l.007-.087A.543.543 0 0 1 5.545 3h9.633L20 7.8v12.662a.534.534 0 0 1-.158.379.548.548 0 0 1-.387.159H11a1 1 0 1 0 0 2h8.455c.674 0 1.32-.267 1.798-.742A2.534 2.534 0 0 0 22 20.462V7.385a1 1 0 0 0-.294-.709l-5.41-5.385Z"/><path d="M10.762 16.647a1 1 0 0 0-1.525-1.294l-4.472 5.271-2.153-1.665a1 1 0 1 0-1.224 1.582l2.91 2.25a1 1 0 0 0 1.374-.144l5.09-6ZM16 10a1 1 0 1 1 0 2H8a1 1 0 1 1 0-2h8ZM12 7a1 1 0 0 0-1-1H8a1 1 0 1 0 0 2h3a1 1 0 0 0 1-1Z"/></g></symbol><symbol id="icon-eds-i-refresh-medium" viewBox="0 0 24 24"><g><path d="M7.831 5.636H6.032A8.76 8.76 0 0 1 9 3.631 8.549 8.549 0 0 1 12.232 3c.603 0 1.192.063 1.76.182C17.979 4.017 21 7.632 21 12a1 1 0 1 0 2 0c0-5.296-3.674-9.746-8.591-10.776A10.61 10.61 0 0 0 5 3.851V2.805a1 1 0 0 0-.987-1H4a1 1 0 0 0-1 1v3.831a1 1 0 0 0 1 1h3.831a1 1 0 0 0 .013-2h-.013ZM17.968 18.364c-1.59 1.632-3.784 2.636-6.2 2.636C6.948 21 3 16.993 3 12a1 1 0 1 0-2 0c0 6.053 4.799 11 10.768 11 2.788 0 5.324-1.082 7.232-2.85v1.045a1 1 0 1 0 2 0v-3.831a1 1 0 0 0-1-1h-3.831a1 1 0 0 0 0 2h1.799Z"/></g></symbol><symbol id="icon-eds-i-search-medium" viewBox="0 0 24 24"><path d="M11 1c5.523 0 10 4.477 10 10 0 2.4-.846 4.604-2.256 6.328l3.963 3.965a1 1 0 0 1-1.414 1.414l-3.965-3.963A9.959 9.959 0 0 1 11 21C5.477 21 1 16.523 1 11S5.477 1 11 1Zm0 2a8 8 0 1 0 0 16 8 8 0 0 0 0-16Z"/></symbol><symbol id="icon-eds-i-settings-medium" viewBox="0 0 24 24"><path d="M11.382 1h1.24a2.508 2.508 0 0 1 2.334 1.63l.523 1.378 1.59.933 1.444-.224c.954-.132 1.89.3 2.422 1.101l.095.155.598 1.066a2.56 2.56 0 0 1-.195 2.848l-.894 1.161v1.896l.92 1.163c.6.768.707 1.812.295 2.674l-.09.17-.606 1.08a2.504 2.504 0 0 1-2.531 1.25l-1.428-.223-1.589.932-.523 1.378a2.512 2.512 0 0 1-2.155 1.625L12.65 23h-1.27a2.508 2.508 0 0 1-2.334-1.63l-.524-1.379-1.59-.933-1.443.225c-.954.132-1.89-.3-2.422-1.101l-.095-.155-.598-1.066a2.56 2.56 0 0 1 .195-2.847l.891-1.161v-1.898l-.919-1.162a2.562 2.562 0 0 1-.295-2.674l.09-.17.606-1.08a2.504 2.504 0 0 1 2.531-1.25l1.43.223 1.618-.938.524-1.375.07-.167A2.507 2.507 0 0 1 11.382 1Zm.003 2a.509.509 0 0 0-.47.338l-.65 1.71a1 1 0 0 1-.434.51L7.6 6.85a1 1 0 0 1-.655.123l-1.762-.275a.497.497 0 0 0-.498.252l-.61 1.088a.562.562 0 0 0 .04.619l1.13 1.43a1 1 0 0 1 .216.62v2.585a1 1 0 0 1-.207.61L4.15 15.339a.568.568 0 0 0-.036.634l.601 1.072a.494.494 0 0 0 .484.26l1.78-.278a1 1 0 0 1 .66.126l2.2 1.292a1 1 0 0 1 .43.507l.648 1.71a.508.508 0 0 0 .467.338h1.263a.51.51 0 0 0 .47-.34l.65-1.708a1 1 0 0 1 .428-.507l2.201-1.292a1 1 0 0 1 .66-.126l1.763.275a.497.497 0 0 0 .498-.252l.61-1.088a.562.562 0 0 0-.04-.619l-1.13-1.43a1 1 0 0 1-.216-.62v-2.585a1 1 0 0 1 .207-.61l1.105-1.437a.568.568 0 0 0 .037-.634l-.601-1.072a.494.494 0 0 0-.484-.26l-1.78.278a1 1 0 0 1-.66-.126l-2.2-1.292a1 1 0 0 1-.43-.507l-.649-1.71A.508.508 0 0 0 12.62 3h-1.234ZM12 8a4 4 0 1 1 0 8 4 4 0 0 1 0-8Zm0 2a2 2 0 1 0 0 4 2 2 0 0 0 0-4Z"/></symbol><symbol id="icon-eds-i-shipping-medium" viewBox="0 0 24 24"><path d="M16.515 2c1.406 0 2.706.728 3.352 1.902l2.02 3.635.02.042.036.089.031.105.012.058.01.073.004.075v11.577c0 .64-.244 1.255-.683 1.713a2.356 2.356 0 0 1-1.701.731H4.386a2.356 2.356 0 0 1-1.702-.731 2.476 2.476 0 0 1-.683-1.713V7.948c.01-.217.083-.43.22-.6L4.2 3.905C4.833 2.755 6.089 2.032 7.486 2h9.029ZM20 9H4v10.556a.49.49 0 0 0 .075.26l.053.07a.356.356 0 0 0 .257.114h15.23c.094 0 .186-.04.258-.115a.477.477 0 0 0 .127-.33V9Zm-2 7.5a1 1 0 0 1 0 2h-4a1 1 0 0 1 0-2h4ZM16.514 4H13v3h6.3l-1.183-2.13c-.288-.522-.908-.87-1.603-.87ZM11 3.999H7.51c-.679.017-1.277.36-1.566.887L4.728 7H11V3.999Z"/></symbol><symbol id="icon-eds-i-step-guide-medium" viewBox="0 0 24 24"><path d="M11.394 9.447a1 1 0 1 0-1.788-.894l-.88 1.759-.019-.02a1 1 0 1 0-1.414 1.415l1 1a1 1 0 0 0 1.601-.26l1.5-3ZM12 11a1 1 0 0 1 1-1h3a1 1 0 1 1 0 2h-3a1 1 0 0 1-1-1ZM12 17a1 1 0 0 1 1-1h3a1 1 0 1 1 0 2h-3a1 1 0 0 1-1-1ZM10.947 14.105a1 1 0 0 1 .447 1.342l-1.5 3a1 1 0 0 1-1.601.26l-1-1a1 1 0 1 1 1.414-1.414l.02.019.879-1.76a1 1 0 0 1 1.341-.447Z"/><path d="M5.545 1A2.542 2.542 0 0 0 3 3.538v16.924A2.542 2.542 0 0 0 5.545 23h12.91A2.542 2.542 0 0 0 21 20.462V7.5a1 1 0 0 0-.293-.707l-5.5-5.5A1 1 0 0 0 14.5 1H5.545ZM5 3.538C5 3.245 5.24 3 5.545 3h8.54L19 7.914v12.547c0 .294-.24.539-.546.539H5.545A.542.542 0 0 1 5 20.462V3.538Z" clip-rule="evenodd"/></symbol><symbol id="icon-eds-i-submission-medium" viewBox="0 0 24 24"><g><path d="M5 3.538C5 3.245 5.24 3 5.545 3h9.633L20 7.8v12.662a.535.535 0 0 1-.158.379.549.549 0 0 1-.387.159H6a1 1 0 0 1-1-1v-2.5a1 1 0 1 0-2 0V20a3 3 0 0 0 3 3h13.455c.673 0 1.32-.266 1.798-.742A2.535 2.535 0 0 0 22 20.462V7.385a1 1 0 0 0-.294-.709l-5.41-5.385A1 1 0 0 0 15.591 1H5.545A2.542 2.542 0 0 0 3 3.538V7a1 1 0 0 0 2 0V3.538Z"/><path d="m13.707 13.707-4 4a1 1 0 0 1-1.414 0l-.083-.094a1 1 0 0 1 .083-1.32L10.585 14 2 14a1 1 0 1 1 0-2l8.583.001-2.29-2.294a1 1 0 0 1 1.414-1.414l4.037 4.04.043.05.043.06.059.098.03.063.031.085.03.113.017.122L14 13l-.004.087-.017.118-.013.056-.034.104-.049.105-.048.081-.07.093-.058.063Z"/></g></symbol><symbol id="icon-eds-i-table-1-medium" viewBox="0 0 24 24"><path d="M4.385 22a2.56 2.56 0 0 1-1.14-.279C2.485 21.341 2 20.614 2 19.615V4.385c0-.315.067-.716.279-1.14C2.659 2.485 3.386 2 4.385 2h15.23c.315 0 .716.067 1.14.279.76.38 1.245 1.107 1.245 2.106v15.23c0 .315-.067.716-.279 1.14-.38.76-1.107 1.245-2.106 1.245H4.385ZM4 19.615c0 .213.034.265.14.317a.71.71 0 0 0 .245.068H8v-4H4v3.615ZM20 16H10v4h9.615c.213 0 .265-.034.317-.14a.71.71 0 0 0 .068-.245V16Zm0-2v-4H10v4h10ZM4 14h4v-4H4v4ZM19.615 4H10v4h10V4.385c0-.213-.034-.265-.14-.317A.71.71 0 0 0 19.615 4ZM8 4H4.385l-.082.002c-.146.01-.19.047-.235.138A.71.71 0 0 0 4 4.385V8h4V4Z"/></symbol><symbol id="icon-eds-i-table-2-medium" viewBox="0 0 24 24"><path d="M4.384 22A2.384 2.384 0 0 1 2 19.616V4.384A2.384 2.384 0 0 1 4.384 2h15.232A2.384 2.384 0 0 1 22 4.384v15.232A2.384 2.384 0 0 1 19.616 22H4.384ZM10 15H4v4.616c0 .212.172.384.384.384H10v-5Zm5 0h-3v5h3v-5Zm5 0h-3v5h2.616a.384.384 0 0 0 .384-.384V15ZM10 9H4v4h6V9Zm5 0h-3v4h3V9Zm5 0h-3v4h3V9Zm-.384-5H4.384A.384.384 0 0 0 4 4.384V7h16V4.384A.384.384 0 0 0 19.616 4Z"/></symbol><symbol id="icon-eds-i-tag-medium" viewBox="0 0 24 24"><path d="m12.621 1.998.127.004L20.496 2a1.5 1.5 0 0 1 1.497 1.355L22 3.5l-.005 7.669c.038.456-.133.905-.447 1.206l-9.02 9.018a2.075 2.075 0 0 1-2.932 0l-6.99-6.99a2.075 2.075 0 0 1 .001-2.933L11.61 2.47c.246-.258.573-.418.881-.46l.131-.011Zm.286 2-8.885 8.886a.075.075 0 0 0 0 .106l6.987 6.988c.03.03.077.03.106 0l8.883-8.883L19.999 4l-7.092-.002ZM16 6.5a1.5 1.5 0 0 1 .144 2.993L16 9.5a1.5 1.5 0 0 1 0-3Z"/></symbol><symbol id="icon-eds-i-trash-medium" viewBox="0 0 24 24"><path d="M12 1c2.717 0 4.913 2.232 4.997 5H21a1 1 0 0 1 0 2h-1v12.5c0 1.389-1.152 2.5-2.556 2.5H6.556C5.152 23 4 21.889 4 20.5V8H3a1 1 0 1 1 0-2h4.003l.001-.051C7.114 3.205 9.3 1 12 1Zm6 7H6v12.5c0 .238.19.448.454.492l.102.008h10.888c.315 0 .556-.232.556-.5V8Zm-4 3a1 1 0 0 1 1 1v6.005a1 1 0 0 1-2 0V12a1 1 0 0 1 1-1Zm-4 0a1 1 0 0 1 1 1v6a1 1 0 0 1-2 0v-6a1 1 0 0 1 1-1Zm2-8c-1.595 0-2.914 1.32-2.996 3h5.991v-.02C14.903 4.31 13.589 3 12 3Z"/></symbol><symbol id="icon-eds-i-user-account-medium" viewBox="0 0 24 24"><path d="M12 1c6.075 0 11 4.925 11 11s-4.925 11-11 11S1 18.075 1 12 5.925 1 12 1Zm0 16c-1.806 0-3.52.994-4.664 2.698A8.947 8.947 0 0 0 12 21a8.958 8.958 0 0 0 4.664-1.301C15.52 17.994 13.806 17 12 17Zm0-14a9 9 0 0 0-6.25 15.476C7.253 16.304 9.54 15 12 15s4.747 1.304 6.25 3.475A9 9 0 0 0 12 3Zm0 3a4 4 0 1 1 0 8 4 4 0 0 1 0-8Zm0 2a2 2 0 1 0 0 4 2 2 0 0 0 0-4Z"/></symbol><symbol id="icon-eds-i-user-add-medium" viewBox="0 0 24 24"><path d="M9 1a5 5 0 1 1 0 10A5 5 0 0 1 9 1Zm0 2a3 3 0 1 0 0 6 3 3 0 0 0 0-6Zm9 10a1 1 0 0 1 1 1v3h3a1 1 0 0 1 0 2h-3v3a1 1 0 0 1-2 0v-3h-3a1 1 0 0 1 0-2h3v-3a1 1 0 0 1 1-1Zm-5.545-.15a1 1 0 1 1-.91 1.78 5.713 5.713 0 0 0-5.705.282c-1.67 1.068-2.728 2.927-2.832 4.956L3.004 20 11.5 20a1 1 0 0 1 .993.883L12.5 21a1 1 0 0 1-1 1H2a1 1 0 0 1-1-1v-.876c.028-2.812 1.446-5.416 3.763-6.897a7.713 7.713 0 0 1 7.692-.378Z"/></symbol><symbol id="icon-eds-i-user-assign-medium" viewBox="0 0 24 24"><path d="M16.226 13.298a1 1 0 0 1 1.414-.01l.084.093a1 1 0 0 1-.073 1.32L15.39 17H22a1 1 0 0 1 0 2h-6.611l2.262 2.298a1 1 0 0 1-1.425 1.404l-3.939-4a1 1 0 0 1 0-1.404l3.94-4Zm-3.771-.449a1 1 0 1 1-.91 1.781 5.713 5.713 0 0 0-5.705.282c-1.67 1.068-2.728 2.927-2.832 4.956L3.004 20 10.5 20a1 1 0 0 1 .993.883L11.5 21a1 1 0 0 1-1 1H2a1 1 0 0 1-1-1v-.876c.028-2.812 1.446-5.416 3.763-6.897a7.713 7.713 0 0 1 7.692-.378ZM9 1a5 5 0 1 1 0 10A5 5 0 0 1 9 1Zm0 2a3 3 0 1 0 0 6 3 3 0 0 0 0-6Z"/></symbol><symbol id="icon-eds-i-user-block-medium" viewBox="0 0 24 24"><path d="M9 1a5 5 0 1 1 0 10A5 5 0 0 1 9 1Zm0 2a3 3 0 1 0 0 6 3 3 0 0 0 0-6Zm9 10a5 5 0 1 1 0 10 5 5 0 0 1 0-10Zm-5.545-.15a1 1 0 1 1-.91 1.78 5.713 5.713 0 0 0-5.705.282c-1.67 1.068-2.728 2.927-2.832 4.956L3.004 20 11.5 20a1 1 0 0 1 .993.883L12.5 21a1 1 0 0 1-1 1H2a1 1 0 0 1-1-1v-.876c.028-2.812 1.446-5.416 3.763-6.897a7.713 7.713 0 0 1 7.692-.378ZM15 18a3 3 0 0 0 4.294 2.707l-4.001-4c-.188.391-.293.83-.293 1.293Zm3-3c-.463 0-.902.105-1.294.293l4.001 4A3 3 0 0 0 18 15Z"/></symbol><symbol id="icon-eds-i-user-check-medium" viewBox="0 0 24 24"><path d="M9 1a5 5 0 1 1 0 10A5 5 0 0 1 9 1Zm0 2a3 3 0 1 0 0 6 3 3 0 0 0 0-6Zm13.647 12.237a1 1 0 0 1 .116 1.41l-5.091 6a1 1 0 0 1-1.375.144l-2.909-2.25a1 1 0 1 1 1.224-1.582l2.153 1.665 4.472-5.271a1 1 0 0 1 1.41-.116Zm-8.139-.977c.22.214.428.44.622.678a1 1 0 1 1-1.548 1.266 6.025 6.025 0 0 0-1.795-1.49.86.86 0 0 1-.163-.048l-.079-.036a5.721 5.721 0 0 0-2.62-.63l-.194.006c-2.76.134-5.022 2.177-5.592 4.864l-.035.175-.035.213c-.03.201-.05.405-.06.61L3.003 20 10 20a1 1 0 0 1 .993.883L11 21a1 1 0 0 1-1 1H2a1 1 0 0 1-1-1v-.876l.005-.223.02-.356.02-.222.03-.248.022-.15c.02-.133.044-.265.071-.397.44-2.178 1.725-4.105 3.595-5.301a7.75 7.75 0 0 1 3.755-1.215l.12-.004a7.908 7.908 0 0 1 5.87 2.252Z"/></symbol><symbol id="icon-eds-i-user-delete-medium" viewBox="0 0 24 24"><path d="M9 1a5 5 0 1 1 0 10A5 5 0 0 1 9 1Zm0 2a3 3 0 1 0 0 6 3 3 0 0 0 0-6ZM4.763 13.227a7.713 7.713 0 0 1 7.692-.378 1 1 0 1 1-.91 1.781 5.713 5.713 0 0 0-5.705.282c-1.67 1.068-2.728 2.927-2.832 4.956L3.004 20H11.5a1 1 0 0 1 .993.883L12.5 21a1 1 0 0 1-1 1H2a1 1 0 0 1-1-1v-.876c.028-2.812 1.446-5.416 3.763-6.897Zm11.421 1.543 2.554 2.553 2.555-2.553a1 1 0 0 1 1.414 1.414l-2.554 2.554 2.554 2.555a1 1 0 0 1-1.414 1.414l-2.555-2.554-2.554 2.554a1 1 0 0 1-1.414-1.414l2.553-2.555-2.553-2.554a1 1 0 0 1 1.414-1.414Z"/></symbol><symbol id="icon-eds-i-user-edit-medium" viewBox="0 0 24 24"><path d="m19.876 10.77 2.831 2.83a1 1 0 0 1 0 1.415l-7.246 7.246a1 1 0 0 1-.572.284l-3.277.446a1 1 0 0 1-1.125-1.13l.461-3.277a1 1 0 0 1 .283-.567l7.23-7.246a1 1 0 0 1 1.415-.001Zm-7.421 2.08a1 1 0 1 1-.91 1.78 5.713 5.713 0 0 0-5.705.282c-1.67 1.068-2.728 2.927-2.832 4.956L3.004 20 7.5 20a1 1 0 0 1 .993.883L8.5 21a1 1 0 0 1-1 1H2a1 1 0 0 1-1-1v-.876c.028-2.812 1.446-5.416 3.763-6.897a7.713 7.713 0 0 1 7.692-.378Zm6.715.042-6.29 6.3-.23 1.639 1.633-.222 6.302-6.302-1.415-1.415ZM9 1a5 5 0 1 1 0 10A5 5 0 0 1 9 1Zm0 2a3 3 0 1 0 0 6 3 3 0 0 0 0-6Z"/></symbol><symbol id="icon-eds-i-user-linked-medium" viewBox="0 0 24 24"><path d="M15.65 6c.31 0 .706.066 1.122.274C17.522 6.65 18 7.366 18 8.35v12.3c0 .31-.066.706-.274 1.122-.375.75-1.092 1.228-2.076 1.228H3.35a2.52 2.52 0 0 1-1.122-.274C1.478 22.35 1 21.634 1 20.65V8.35c0-.31.066-.706.274-1.122C1.65 6.478 2.366 6 3.35 6h12.3Zm0 2-12.376.002c-.134.007-.17.04-.21.12A.672.672 0 0 0 3 8.35v12.3c0 .198.028.24.122.287.09.044.2.063.228.063h.887c.788-2.269 2.814-3.5 5.263-3.5 2.45 0 4.475 1.231 5.263 3.5h.887c.198 0 .24-.028.287-.122.044-.09.063-.2.063-.228V8.35c0-.198-.028-.24-.122-.287A.672.672 0 0 0 15.65 8ZM9.5 19.5c-1.36 0-2.447.51-3.06 1.5h6.12c-.613-.99-1.7-1.5-3.06-1.5ZM20.65 1A2.35 2.35 0 0 1 23 3.348V15.65A2.35 2.35 0 0 1 20.65 18H20a1 1 0 0 1 0-2h.65a.35.35 0 0 0 .35-.35V3.348A.35.35 0 0 0 20.65 3H8.35a.35.35 0 0 0-.35.348V4a1 1 0 1 1-2 0v-.652A2.35 2.35 0 0 1 8.35 1h12.3ZM9.5 10a3.5 3.5 0 1 1 0 7 3.5 3.5 0 0 1 0-7Zm0 2a1.5 1.5 0 1 0 0 3 1.5 1.5 0 0 0 0-3Z"/></symbol><symbol id="icon-eds-i-user-multiple-medium" viewBox="0 0 24 24"><path d="M9 1a5 5 0 1 1 0 10A5 5 0 0 1 9 1Zm6 0a5 5 0 0 1 0 10 1 1 0 0 1-.117-1.993L15 9a3 3 0 0 0 0-6 1 1 0 0 1 0-2ZM9 3a3 3 0 1 0 0 6 3 3 0 0 0 0-6Zm8.857 9.545a7.99 7.99 0 0 1 2.651 1.715A8.31 8.31 0 0 1 23 20.134V21a1 1 0 0 1-1 1h-3a1 1 0 0 1 0-2h1.995l-.005-.153a6.307 6.307 0 0 0-1.673-3.945l-.204-.209a5.99 5.99 0 0 0-1.988-1.287 1 1 0 1 1 .732-1.861Zm-3.349 1.715A8.31 8.31 0 0 1 17 20.134V21a1 1 0 0 1-1 1H2a1 1 0 0 1-1-1v-.877c.044-4.343 3.387-7.908 7.638-8.115a7.908 7.908 0 0 1 5.87 2.252ZM9.016 14l-.285.006c-3.104.15-5.58 2.718-5.725 5.9L3.004 20h11.991l-.005-.153a6.307 6.307 0 0 0-1.673-3.945l-.204-.209A5.924 5.924 0 0 0 9.3 14.008L9.016 14Z"/></symbol><symbol id="icon-eds-i-user-notify-medium" viewBox="0 0 24 24"><path d="M9 1a5 5 0 1 1 0 10A5 5 0 0 1 9 1Zm0 2a3 3 0 1 0 0 6 3 3 0 0 0 0-6Zm10 18v1a1 1 0 0 1-2 0v-1h-3a1 1 0 0 1 0-2v-2.818C14 13.885 15.777 12 18 12s4 1.885 4 4.182V19a1 1 0 0 1 0 2h-3Zm-6.545-8.15a1 1 0 1 1-.91 1.78 5.713 5.713 0 0 0-5.705.282c-1.67 1.068-2.728 2.927-2.832 4.956L3.004 20 11.5 20a1 1 0 0 1 .993.883L12.5 21a1 1 0 0 1-1 1H2a1 1 0 0 1-1-1v-.876c.028-2.812 1.446-5.416 3.763-6.897a7.713 7.713 0 0 1 7.692-.378ZM18 14c-1.091 0-2 .964-2 2.182V19h4v-2.818c0-1.165-.832-2.098-1.859-2.177L18 14Z"/></symbol><symbol id="icon-eds-i-user-remove-medium" viewBox="0 0 24 24"><path d="M9 1a5 5 0 1 1 0 10A5 5 0 0 1 9 1Zm0 2a3 3 0 1 0 0 6 3 3 0 0 0 0-6Zm3.455 9.85a1 1 0 1 1-.91 1.78 5.713 5.713 0 0 0-5.705.282c-1.67 1.068-2.728 2.927-2.832 4.956L3.004 20 11.5 20a1 1 0 0 1 .993.883L12.5 21a1 1 0 0 1-1 1H2a1 1 0 0 1-1-1v-.876c.028-2.812 1.446-5.416 3.763-6.897a7.713 7.713 0 0 1 7.692-.378ZM22 17a1 1 0 0 1 0 2h-8a1 1 0 0 1 0-2h8Z"/></symbol><symbol id="icon-eds-i-user-single-medium" viewBox="0 0 24 24"><path d="M12 1a5 5 0 1 1 0 10 5 5 0 0 1 0-10Zm0 2a3 3 0 1 0 0 6 3 3 0 0 0 0-6Zm-.406 9.008a8.965 8.965 0 0 1 6.596 2.494A9.161 9.161 0 0 1 21 21.025V22a1 1 0 0 1-1 1H4a1 1 0 0 1-1-1v-.985c.05-4.825 3.815-8.777 8.594-9.007Zm.39 1.992-.299.006c-3.63.175-6.518 3.127-6.678 6.775L5 21h13.998l-.009-.268a7.157 7.157 0 0 0-1.97-4.573l-.214-.213A6.967 6.967 0 0 0 11.984 14Z"/></symbol><symbol id="icon-eds-i-warning-circle-medium" viewBox="0 0 24 24"><path d="M12 1c6.075 0 11 4.925 11 11s-4.925 11-11 11S1 18.075 1 12 5.925 1 12 1Zm0 2a9 9 0 1 0 0 18 9 9 0 0 0 0-18Zm0 11.5a1.5 1.5 0 0 1 .144 2.993L12 17.5a1.5 1.5 0 0 1 0-3ZM12 6a1 1 0 0 1 1 1v5a1 1 0 0 1-2 0V7a1 1 0 0 1 1-1Z"/></symbol><symbol id="icon-eds-i-warning-filled-medium" viewBox="0 0 24 24"><path d="M12 1c6.075 0 11 4.925 11 11s-4.925 11-11 11S1 18.075 1 12 5.925 1 12 1Zm0 13.5a1.5 1.5 0 0 0 0 3l.144-.007A1.5 1.5 0 0 0 12 14.5ZM12 6a1 1 0 0 0-1 1v5a1 1 0 0 0 2 0V7a1 1 0 0 0-1-1Z"/></symbol><symbol id="icon-chevron-left-medium" viewBox="0 0 24 24"><path d="M15.7194 3.3054C15.3358 2.90809 14.7027 2.89699 14.3054 3.28061L6.54342 10.7757C6.19804 11.09 6 11.5335 6 12C6 12.4665 6.19804 12.91 6.5218 13.204L14.3054 20.7194C14.7027 21.103 15.3358 21.0919 15.7194 20.6946C16.103 20.2973 16.0919 19.6642 15.6946 19.2806L8.155 12L15.6946 4.71939C16.0614 4.36528 16.099 3.79863 15.8009 3.40105L15.7194 3.3054Z"/></symbol><symbol id="icon-chevron-right-medium" viewBox="0 0 24 24"><path d="M8.28061 3.3054C8.66423 2.90809 9.29729 2.89699 9.6946 3.28061L17.4566 10.7757C17.802 11.09 18 11.5335 18 12C18 12.4665 17.802 12.91 17.4782 13.204L9.6946 20.7194C9.29729 21.103 8.66423 21.0919 8.28061 20.6946C7.89699 20.2973 7.90809 19.6642 8.3054 19.2806L15.845 12L8.3054 4.71939C7.93865 4.36528 7.90098 3.79863 8.19908 3.40105L8.28061 3.3054Z"/></symbol><symbol id="icon-eds-alerts" viewBox="0 0 32 32"><path d="M28 12.667c.736 0 1.333.597 1.333 1.333v13.333A3.333 3.333 0 0 1 26 30.667H6a3.333 3.333 0 0 1-3.333-3.334V14a1.333 1.333 0 1 1 2.666 0v1.252L16 21.769l10.667-6.518V14c0-.736.597-1.333 1.333-1.333Zm-1.333 5.71-9.972 6.094c-.427.26-.963.26-1.39 0l-9.972-6.094v8.956c0 .368.299.667.667.667h20a.667.667 0 0 0 .667-.667v-8.956ZM19.333 12a1.333 1.333 0 1 1 0 2.667h-6.666a1.333 1.333 0 1 1 0-2.667h6.666Zm4-10.667a3.333 3.333 0 0 1 3.334 3.334v6.666a1.333 1.333 0 1 1-2.667 0V4.667A.667.667 0 0 0 23.333 4H8.667A.667.667 0 0 0 8 4.667v6.666a1.333 1.333 0 1 1-2.667 0V4.667a3.333 3.333 0 0 1 3.334-3.334h14.666Zm-4 5.334a1.333 1.333 0 0 1 0 2.666h-6.666a1.333 1.333 0 1 1 0-2.666h6.666Z"/></symbol><symbol id="icon-eds-arrow-up" viewBox="0 0 24 24"><path fill-rule="evenodd" d="m13.002 7.408 4.88 4.88a.99.99 0 0 0 1.32.08l.09-.08c.39-.39.39-1.03 0-1.42l-6.58-6.58a1.01 1.01 0 0 0-1.42 0l-6.58 6.58a1 1 0 0 0-.09 1.32l.08.1a1 1 0 0 0 1.42-.01l4.88-4.87v11.59a.99.99 0 0 0 .88.99l.12.01c.55 0 1-.45 1-1V7.408z" class="layer"/></symbol><symbol id="icon-eds-checklist" viewBox="0 0 32 32"><path d="M19.2 1.333a3.468 3.468 0 0 1 3.381 2.699L24.667 4C26.515 4 28 5.52 28 7.38v19.906c0 1.86-1.485 3.38-3.333 3.38H7.333c-1.848 0-3.333-1.52-3.333-3.38V7.38C4 5.52 5.485 4 7.333 4h2.093A3.468 3.468 0 0 1 12.8 1.333h6.4ZM9.426 6.667H7.333c-.36 0-.666.312-.666.713v19.906c0 .401.305.714.666.714h17.334c.36 0 .666-.313.666-.714V7.38c0-.4-.305-.713-.646-.714l-2.121.033A3.468 3.468 0 0 1 19.2 9.333h-6.4a3.468 3.468 0 0 1-3.374-2.666Zm12.715 5.606c.586.446.7 1.283.253 1.868l-7.111 9.334a1.333 1.333 0 0 1-1.792.306l-3.556-2.333a1.333 1.333 0 1 1 1.463-2.23l2.517 1.651 6.358-8.344a1.333 1.333 0 0 1 1.868-.252ZM19.2 4h-6.4a.8.8 0 0 0-.8.8v1.067a.8.8 0 0 0 .8.8h6.4a.8.8 0 0 0 .8-.8V4.8a.8.8 0 0 0-.8-.8Z"/></symbol><symbol id="icon-eds-citation" viewBox="0 0 36 36"><path d="M23.25 1.5a1.5 1.5 0 0 1 1.06.44l8.25 8.25a1.5 1.5 0 0 1 .44 1.06v19.5c0 2.105-1.645 3.75-3.75 3.75H18a1.5 1.5 0 0 1 0-3h11.25c.448 0 .75-.302.75-.75V11.873L22.628 4.5H8.31a.811.811 0 0 0-.8.68l-.011.13V16.5a1.5 1.5 0 0 1-3 0V5.31A3.81 3.81 0 0 1 8.31 1.5h14.94ZM8.223 20.358a.984.984 0 0 1-.192 1.378l-.048.034c-.54.36-.942.676-1.206.951-.59.614-.885 1.395-.885 2.343.115-.028.288-.042.518-.042.662 0 1.26.237 1.791.711.533.474.799 1.074.799 1.799 0 .753-.259 1.352-.777 1.799-.518.446-1.151.669-1.9.669-1.006 0-1.812-.293-2.417-.878C3.302 28.536 3 27.657 3 26.486c0-1.115.165-2.085.496-2.907.331-.823.734-1.513 1.209-2.071.475-.558.971-.997 1.49-1.318a6.01 6.01 0 0 1 .347-.2 1.321 1.321 0 0 1 1.681.368Zm7.5 0a.984.984 0 0 1-.192 1.378l-.048.034c-.54.36-.942.676-1.206.951-.59.614-.885 1.395-.885 2.343.115-.028.288-.042.518-.042.662 0 1.26.237 1.791.711.533.474.799 1.074.799 1.799 0 .753-.259 1.352-.777 1.799-.518.446-1.151.669-1.9.669-1.006 0-1.812-.293-2.417-.878-.604-.586-.906-1.465-.906-2.636 0-1.115.165-2.085.496-2.907.331-.823.734-1.513 1.209-2.071.475-.558.971-.997 1.49-1.318a6.01 6.01 0 0 1 .347-.2 1.321 1.321 0 0 1 1.681.368Z"/></symbol><symbol id="icon-eds-i-access-indicator" viewBox="0 0 16 16"><circle cx="4.5" cy="11.5" r="3.5" style="fill:currentColor"/><path fill-rule="evenodd" d="M4 3v3a1 1 0 0 1-2 0V2.923C2 1.875 2.84 1 3.909 1h5.909a1 1 0 0 1 .713.298l3.181 3.231a1 1 0 0 1 .288.702v7.846c0 .505-.197.993-.554 1.354a1.902 1.902 0 0 1-1.355.569H10a1 1 0 1 1 0-2h2V5.64L9.4 3H4Z" clip-rule="evenodd" style="fill:#222"/></symbol><symbol id="icon-eds-i-github-medium" viewBox="0 0 24 24"><path d="M 11.964844 0 C 5.347656 0 0 5.269531 0 11.792969 C 0 17.003906 3.425781 21.417969 8.179688 22.976562 C 8.773438 23.09375 8.992188 22.722656 8.992188 22.410156 C 8.992188 22.136719 8.972656 21.203125 8.972656 20.226562 C 5.644531 20.929688 4.953125 18.820312 4.953125 18.820312 C 4.417969 17.453125 3.625 17.101562 3.625 17.101562 C 2.535156 16.378906 3.703125 16.378906 3.703125 16.378906 C 4.914062 16.457031 5.546875 17.589844 5.546875 17.589844 C 6.617188 19.386719 8.339844 18.878906 9.03125 18.566406 C 9.132812 17.804688 9.449219 17.277344 9.785156 16.984375 C 7.132812 16.710938 4.339844 15.695312 4.339844 11.167969 C 4.339844 9.878906 4.8125 8.824219 5.566406 8.003906 C 5.445312 7.710938 5.03125 6.5 5.683594 4.878906 C 5.683594 4.878906 6.695312 4.566406 8.972656 6.089844 C 9.949219 5.832031 10.953125 5.703125 11.964844 5.699219 C 12.972656 5.699219 14.003906 5.835938 14.957031 6.089844 C 17.234375 4.566406 18.242188 4.878906 18.242188 4.878906 C 18.898438 6.5 18.480469 7.710938 18.363281 8.003906 C 19.136719 8.824219 19.589844 9.878906 19.589844 11.167969 C 19.589844 15.695312 16.796875 16.691406 14.125 16.984375 C 14.558594 17.355469 14.933594 18.058594 14.933594 19.171875 C 14.933594 20.753906 14.914062 22.019531 14.914062 22.410156 C 14.914062 22.722656 15.132812 23.09375 15.726562 22.976562 C 20.480469 21.414062 23.910156 17.003906 23.910156 11.792969 C 23.929688 5.269531 18.558594 0 11.964844 0 Z M 11.964844 0 "/></symbol><symbol id="icon-eds-i-limited-access" viewBox="0 0 16 16"><path fill-rule="evenodd" d="M4 3v3a1 1 0 0 1-2 0V2.923C2 1.875 2.84 1 3.909 1h5.909a1 1 0 0 1 .713.298l3.181 3.231a1 1 0 0 1 .288.702V6a1 1 0 1 1-2 0v-.36L9.4 3H4ZM3 8a1 1 0 0 1 1 1v1a1 1 0 1 1-2 0V9a1 1 0 0 1 1-1Zm10 0a1 1 0 0 1 1 1v1a1 1 0 1 1-2 0V9a1 1 0 0 1 1-1Zm-3.5 6a1 1 0 0 1-1 1h-1a1 1 0 1 1 0-2h1a1 1 0 0 1 1 1Zm2.441-1a1 1 0 0 1 2 0c0 .73-.246 1.306-.706 1.664a1.61 1.61 0 0 1-.876.334l-.032.002H11.5a1 1 0 1 1 0-2h.441ZM4 13a1 1 0 0 0-2 0c0 .73.247 1.306.706 1.664a1.609 1.609 0 0 0 .876.334l.032.002H4.5a1 1 0 1 0 0-2H4Z" clip-rule="evenodd"/></symbol><symbol id="icon-eds-i-subjects-medium" viewBox="0 0 24 24"><g id="icon-subjects-copy" stroke="none" stroke-width="1" fill-rule="evenodd"><path d="M13.3846154,2 C14.7015971,2 15.7692308,3.06762994 15.7692308,4.38461538 L15.7692308,7.15384615 C15.7692308,8.47082629 14.7015955,9.53846154 13.3846154,9.53846154 L13.1038388,9.53925278 C13.2061091,9.85347965 13.3815528,10.1423885 13.6195822,10.3804178 C13.9722182,10.7330539 14.436524,10.9483278 14.9293854,10.9918129 L15.1153846,11 C16.2068332,11 17.2535347,11.433562 18.0254647,12.2054189 C18.6411944,12.8212361 19.0416785,13.6120766 19.1784166,14.4609738 L19.6153846,14.4615385 C20.932386,14.4615385 22,15.5291672 22,16.8461538 L22,19.6153846 C22,20.9323924 20.9323924,22 19.6153846,22 L16.8461538,22 C15.5291672,22 14.4615385,20.932386 14.4615385,19.6153846 L14.4615385,16.8461538 C14.4615385,15.5291737 15.5291737,14.4615385 16.8461538,14.4615385 L17.126925,14.460779 C17.0246537,14.1465537 16.8492179,13.857633 16.6112344,13.6196157 C16.2144418,13.2228606 15.6764136,13 15.1153846,13 C14.0239122,13 12.9771569,12.5664197 12.2053686,11.7946314 C12.1335167,11.7227795 12.0645962,11.6485444 11.9986839,11.5721119 C11.9354038,11.6485444 11.8664833,11.7227795 11.7946314,11.7946314 C11.0228431,12.5664197 9.97608778,13 8.88461538,13 C8.323576,13 7.78552852,13.2228666 7.38881294,13.6195822 C7.15078359,13.8576115 6.97533988,14.1465203 6.8730696,14.4607472 L7.15384615,14.4615385 C8.47082629,14.4615385 9.53846154,15.5291737 9.53846154,16.8461538 L9.53846154,19.6153846 C9.53846154,20.932386 8.47083276,22 7.15384615,22 L4.38461538,22 C3.06762347,22 2,20.9323876 2,19.6153846 L2,16.8461538 C2,15.5291721 3.06762994,14.4615385 4.38461538,14.4615385 L4.8215823,14.4609378 C4.95831893,13.6120029 5.3588057,12.8211623 5.97459937,12.2053686 C6.69125996,11.488708 7.64500941,11.0636656 8.6514968,11.0066017 L8.88461538,11 C9.44565477,11 9.98370225,10.7771334 10.3804178,10.3804178 C10.6184472,10.1423885 10.7938909,9.85347965 10.8961612,9.53925278 L10.6153846,9.53846154 C9.29840448,9.53846154 8.23076923,8.47082629 8.23076923,7.15384615 L8.23076923,4.38461538 C8.23076923,3.06762994 9.29840286,2 10.6153846,2 L13.3846154,2 Z M7.15384615,16.4615385 L4.38461538,16.4615385 C4.17220099,16.4615385 4,16.63374 4,16.8461538 L4,19.6153846 C4,19.8278134 4.17218833,20 4.38461538,20 L7.15384615,20 C7.36626945,20 7.53846154,19.8278103 7.53846154,19.6153846 L7.53846154,16.8461538 C7.53846154,16.6337432 7.36625679,16.4615385 7.15384615,16.4615385 Z M19.6153846,16.4615385 L16.8461538,16.4615385 C16.6337432,16.4615385 16.4615385,16.6337432 16.4615385,16.8461538 L16.4615385,19.6153846 C16.4615385,19.8278103 16.6337306,20 16.8461538,20 L19.6153846,20 C19.8278229,20 20,19.8278229 20,19.6153846 L20,16.8461538 C20,16.6337306 19.8278103,16.4615385 19.6153846,16.4615385 Z M13.3846154,4 L10.6153846,4 C10.4029708,4 10.2307692,4.17220099 10.2307692,4.38461538 L10.2307692,7.15384615 C10.2307692,7.36625679 10.402974,7.53846154 10.6153846,7.53846154 L13.3846154,7.53846154 C13.597026,7.53846154 13.7692308,7.36625679 13.7692308,7.15384615 L13.7692308,4.38461538 C13.7692308,4.17220099 13.5970292,4 13.3846154,4 Z" id="Shape" fill-rule="nonzero"/></g></symbol><symbol id="icon-eds-small-arrow-left" viewBox="0 0 16 17"><path stroke="currentColor" stroke-linecap="round" stroke-linejoin="round" stroke-width="2" d="M14 8.092H2m0 0L8 2M2 8.092l6 6.035"/></symbol><symbol id="icon-eds-small-arrow-right" viewBox="0 0 16 16"><g fill-rule="evenodd" stroke="currentColor" stroke-linecap="round" stroke-linejoin="round" stroke-width="2"><path d="M2 8.092h12M8 2l6 6.092M8 14.127l6-6.035"/></g></symbol><symbol id="icon-orcid-logo" viewBox="0 0 40 40"><path fill-rule="evenodd" d="M12.281 10.453c.875 0 1.578-.719 1.578-1.578 0-.86-.703-1.578-1.578-1.578-.875 0-1.578.703-1.578 1.578 0 .86.703 1.578 1.578 1.578Zm-1.203 18.641h2.406V12.359h-2.406v16.735Z"/><path fill-rule="evenodd" d="M17.016 12.36h6.5c6.187 0 8.906 4.421 8.906 8.374 0 4.297-3.36 8.375-8.875 8.375h-6.531V12.36Zm6.234 14.578h-3.828V14.53h3.703c4.688 0 6.828 2.844 6.828 6.203 0 2.063-1.25 6.203-6.703 6.203Z" clip-rule="evenodd"/></symbol></svg> </div> </body> </html>

CINXE.COM

Zebrafish as a Model for Investigating Antiviral Innate Immunity | SpringerLink