StemXVivo Adipogenic Supplement (100X) (CCM011) by R&D Systems, Part of Bio-Techne

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class="col sub-menu-wrapper"> <div class="sub-menu-header"> Product & Instrument Tools<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/resources/cofa-finder-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/cofa-finder-tool">Certificate of Analysis (COA) Finder Tool</a> <a href="/resources/calculators" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7416">Calculators</a> <a href="/resources/flow-cytometry-panel-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="node/8821">Flow Cytometry Panel Builder</a> <a href="/luminex-assay-customization-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="luminex-assay-customization-tool">Luminex Assay Customization Tool</a> <a href="/resources/product-insert-lookup-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/product-insert-lookup-tool">Product Insert Lookup 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id="pp-page-title-brand-wrapper"> <div id="header-subheader" data-section="section-base--header-subheader"> <div class="row" id="pp-page-title-brand-wrapper"> <div class="col-lg-9 col-md-12"> <h1 class="font-weight-bold"> StemXVivo Adipogenic Supplement (100X) </h1> <h2 class="font-weight-normal h4"> R&D Systems, part of Bio-Techne <span id="product-code"> | Catalog # <span class="font-weight-bold">CCM011</span> </span> </h2> <div id="pp-reviews-citations-row"> <div id="header_left" class="row"> <div id="submit_review_wrapper" class="col-auto d-flex align-self-center"> <div id="submit_review"> <a href="/reviews/CCM011" class="submit_review" rel="nofollow"> <span class="icon-plus-circle d-none d-lg-inline pr-1"></span> Submit a review </a> </div> </div> <div id="citation_quick_link_wrapper" class="col-auto d-flex align-self-center"> <div id="citation_quicklink"> <a href="#citations" id="citation_count" data-tab="2" class="citations"> <span class="icon-icon-product-citations d-none d-lg-inline pr-1"></span> Citations (40)  </a> </div> </div> <div class="col-auto d-flex align-self-center"> <div> <a href="#product-documents" class="datasheet_link product-documents" rel="nofollow">Product Datasheet / COA / SDS</a> </div> </div> </div> </div> <div id="new_version_available"> </div> <div class="row mb-2" id="extended-description"> <div class="col-md-10 extended-description-wrapper" id="extended-description-wrapper"> For the differentiation of human, mouse, or rat Mesenchymal Stem Cells (MSCs) into adipocytes </div> </div> </div> <div class="col-lg-3 col-md-12"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" alt="R&D Systems, part of Bio-Techne" class="d-flex h-100 w-100"> </div> </div> </div> </div> <div id="product_alerts_wrapper"><article class="product-data-wrapper"> </article> </div> <div class="datapoint_list" id="key_details"><h3>Key Product Details</h3></div> </div> <div class="row border-top mb-2" id="images_atc_orderinfo_wrapper"><div class="col-xl-9 mt-2" id="image_atc_keydetails_wrapper"><div class="row" id="image_atc_wrapper"><div class="col-xl-4 order-xl-first" id="image_wrapper"><article class="product-data-wrapper"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="https://resources.bio-techne.com/images/products/StemXVivo_Adipogenic_Supplement_CCM011_Human_FABP4_ICC_Large_540.png" alt="Detection of FABP4 in Human MSC-differentiated Adipocytes." title="Detection of FABP4 in Human MSC-differentiated Adipocytes." data-caption="Human MSCs were differentiated for 14 days using the Human/Mouse/Rat StemXVivo<sup>®</sup>Osteogenic/Adipogenic Base Media (Catalog # <a class="NoLineLink" href="/p/cell-culture/stemxvivo-osteogenic-adipogenic-base-media_ccm007" target="_self">CCM007</a>) and Human/Mouse/Rat StemXVivo<sup>®</sup>Adipogenic Supplement (Catalog # CCM011). Mature differentiated adipoctyes were detected with a Goat Anti-Human FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # <a class="NoLineLink" href="/p/antibodies/human-fabp4-a-fabp-antibody_af3150" target="_self">AF3150</a>). The cells were stained with a NorthernLights<sup>™</sup>557-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # <a class="NoLineLink" href="/p/secondary-antibodies/goat-igg-northernlights-nl557-conjugated-antibody_nl001" target="_self">NL001</a>; red) and the nuclei were counterstained with DAPI (blue)." data-badges="" data-application="Immunocytochemistry" width="250" height="250" fetchpriority=high as=image class="search_result_thumbnail_image" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(4)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/StemXVivo_Adipogenic_Supplement_CCM011_Mouse_FABP4_ICC_Large_540.png" data-alt="Detection of FABP4 in Mouse MSC-differentiated Adipocytes." title="Detection of FABP4 in Mouse MSC-differentiated Adipocytes." data-caption="Mouse MSCs were differentiated for 14 days using the Human/Mouse/Rat StemXVivo<sup>®</sup>Osteogenic/Adipogenic Base Media (Catalog # <a class="NoLineLink" href="/p/cell-culture/stemxvivo-osteogenic-adipogenic-base-media_ccm007" target="_self">CCM007</a>) and Human/Mouse/Rat StemXVivo<sup>®</sup>Adipogenic Supplement (Catalog # CCM011). Mature differentiated adipoctyes were detected with a Goat Anti-Mouse FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # <a class="NoLineLink" href="/p/antibodies/mouse-rat-fabp4-a-fabp-antibody_af1443" target="_self">AF1443</a>). The cells were stained with a NorthernLights<sup>™</sup>557-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # <a class="NoLineLink" href="/p/secondary-antibodies/goat-igg-northernlights-nl557-conjugated-antibody_nl001" target="_self">NL001</a>; red) and the nuclei were counterstained with DAPI (blue)." data-badges="" data-application="Immunocytochemistry" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/StemXVivo_Adipogenic_Supplement_CCM011_Rat_FABP4_ICC_Large_540.png" data-alt="Detection of FABP4 in Rat MSC-differentiated Adipocytes." title="Detection of FABP4 in Rat MSC-differentiated Adipocytes." data-caption="Rat MSCs were differentiated for 14 days using the Human/Mouse/Rat StemXVivo<sup>®</sup>Osteogenic/Adipogenic Base Media (Catalog # <a class="NoLineLink" href="/p/cell-culture/stemxvivo-osteogenic-adipogenic-base-media_ccm007" target="_self">CCM007</a>) and Human/Mouse/Rat StemXVivo<sup>®</sup>Adipogenic Supplement (Catalog # CCM011). Mature differentiated adipoctyes were detected with a Goat Anti-Mouse FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # <a class="NoLineLink" href="/p/antibodies/mouse-rat-fabp4-a-fabp-antibody_af1443" target="_self">AF1443</a>). The cells were stained with a NorthernLights<sup>™</sup>557-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # <a class="NoLineLink" href="/p/secondary-antibodies/goat-igg-northernlights-nl557-conjugated-antibody_nl001" target="_self">NL001</a>; red) and the nuclei were counterstained with DAPI (blue)." data-badges="" data-application="Immunocytochemistry" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/ccm011_stemxvivo-adipogenic-supplement-100x-46202010313.jpg" data-alt="StemXVivo Adipogenic Supplement (100X)" title="StemXVivo Adipogenic Supplement (100X)" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> </div> </div> </article> </div> <div class="col-xl-8" id="atc_wrapper"><article class="product-data-wrapper"> <div class="atc-container"> <div class="atc-block" data-type="ds" data-blockid="ProductCart" > <form action="/commerce/addtocart" method="post" 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</a> </lite-youtube> </div> </article> </div> </article> </div> <div id="product_ads_wrapper"><article class="product-data-wrapper"> </article> </div> </div> </div> </div> <div class="tab-content rounded-bottom border-0 mb-3 shadow"><div id="about-data-tab-tab"></div> <ul class="nav nav-tabs nav-fill " id="productTab" role="tablist"> <li class="nav-item"> <a class="nav-link active" id="technical-data-tab-tab" href="#technical-data-tab" data-toggle="tab" role="tab" aria-controls="region-4821584720927" aria-selected="true" >Technical Data</a> </li> <li class="nav-item"> <a class="nav-link" id="procedure-tab-tab" href="#procedure-tab" data-toggle="tab" role="tab" aria-controls="region-2811585257724" aria-selected="false" >Procedure</a> </li> <li class="nav-item"> <a class="nav-link" id="citations-reviews-faqs-tab-tab" href="#citations-reviews-faqs-tab" data-toggle="tab" role="tab" aria-controls="region-7631584720992" aria-selected="false" >Citations / Reviews / FAQs</a> </li> </ul> <div 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class="nav-link py-0 px-2 jump-anchor" href="#preparation--storage" id="preparation--storage_li" >Preparation & Storage</a> </li> <li id="product-documents_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 product-documents datasheet_static_link" href="#product-documents" id="product-documents_li" >Product Datasheets</a> </li> </div> <div id="product-summary" class="nav-link px-0" data-anchor-label="Product Summary"><div id="product_title_summary"><h3>Summary for StemXVivo Adipogenic Supplement (100X)</h3><article class="product-data-wrapper"> <html><body><div class="ds_lightBlueBar">Kit Summary</div> <p>Media supplement to induce the differentiation of human MSCs into osteocytes. For use with Human/Mouse/Rat StemXVivo® Osteogenic/Adipogenic Base Media (Catalog # CCM007).</p> <div class="grid_6"> <h3>Key Benefits</h3> <ul> <li>Supports induction of adipogenesis in MSCs</li> <li>Defined supplement to reduce experimental variation</li> <li>Developed and optimized using MSCs</li> </ul> </div> <div class="clear"> </div> <p> </p> <h3>Why Induce Adipogenesis in MSCs with a Defined Media Supplement?</h3> <p>Despite the well-characterized factors and protocols used to differentiate mesenchymal stem/stromal cells (MSCs) into adipocytes, differentiation efficiencies can vary depending on the quality of the MSC starting population and the reagents used to expand and differentiate MSCs.</p> <h4>StemXVivo<sup>®</sup> Adipogenic Supplement:</h4> <ul> <li>Contains high quality differentiation factors to drive reproducible and efficient MSC adipogenesis.</li> <li>Is defined to reduce unwanted experimental variability.</li> <li>Has been developed and optimized using MSCs.</li> </ul> <div class="clear"> </div> <p> </p> <div class="ds_lightBlueBar">Mesenchymal Stromal Cells or Mesenchymal Stem Cells?</div> <p>The term ‘mesenchymal stromal cells’ is commonly used to describe a heterogeneous population of cultured cells that are adherent to plastic, have a distinct morphology, and express a specific set of marker proteins. Within this heterogeneous population are cells referred to as ‘mesenchymal stem cells.’</p> <p>Mesenchymal stem cells are multipotent, self-renewing cells that have the ability to differentiate into adipocytes, chondrocytes, and osteoblasts when cultured <em>in vitro</em>. <a href="#msc-nomenclature">Read More about MSC Nomenclature</a></p> <h4>StemXVivo<sup>®</sup> Adipogenic Supplement Components</h4> <p>This media supplement contains high quality factors to drive human, mouse, or rat MSC differentiation into adipocytes.<a id="data-examples" name="data-examples"></a></p> <ul class="genericPSP_StandardList"> <li>This supplement requires media (not included), such as Human/Mouse/Rat StemXVivo<sup>®</sup> Osteogenic/Adipogenic Base Media (Catalog # <a href="/p/cell-culture/stemxvivo-osteogenic-adipogenic-base-media_ccm007">CCM007</a>) or equivalent.</li> <li>The quantity of adipogenic media supplement supplied is sufficient to make 350 mL of media for differentiation.</li> </ul> <p> </p> <a id="msc-nomenclature" name="msc-nomenclature"></a> <h4>2006 Proposed Change to MSC Nomenclature</h4> <p>Although mesenchymal stromal cells were once referred to as ‘mesenchymal stem cells’, a change to ‘mesenchymal stromal cells’ was proposed by the International Society for Cellular Therapy in 2006.<sup id="fnref:fn1"><a href="#fn:fn1" rel="footnote">1</a></sup></p> <p>The change in nomenclature originates from two important factors:</p> <ul> <li>Methods used to isolate mesenchymal stem cells yield a heterogeneous population of cells with only a fraction of these cells demonstrating multipotency.</li> <li>The absence of direct evidence that mesenchymal stem cells can self-renew and differentiate <em>in vivo</em>.</li> </ul> <h4>Use of Mesenchymal Stem and Stromal Cell Terminology</h4> <p>Data supporting MSC self-renewal and multipotency have been obtained using <em>in vitro</em> conditions, which does not adequately reflect the in vivo environment. The lack of <em>in vivo</em> data has led some researchers to question the validity of the term ‘mesenchymal stem cell’ providing further support for the use of ‘mesenchymal stromal cells’ to describe MSCs.<sup id="fnref:fn2"><a href="#fn:fn2" rel="footnote">2</a></sup> While ‘mesenchymal stromal cells’ may be the more scientifically accurate term for MSCs, the two terms are often used interchangeably in the literature. R&D Systems recognizes the use of both mesenchymal stem cells and mesenchymal stromal cells and uses ‘MSC’ to indicate mesenchymal stem/stromal cells to account for both designations.</p> <h4>Definitions of Mesenchymal Stromal Cells and Mesenchymal Stem Cells</h4> <ul> <li>Mesenchymal Stromal Cells – A heterogeneous population of cultured cells with similar characteristics such as the ability to adhere to plastic and the expression of specific marker proteins.</li> <li>Mesenchymal Stem Cells – A subpopulation of mesenchymal stromal cells that have the capacity to self-renew and differentiate into mesodermal lineages when cultured <em>in vitro</em>. The capacity to self-renew and differentiate <em>in vivo</em> has yet to be clearly demonstrated for mesenchymal stem cells.</li> </ul> <h4>References</h4> <ul> <li id="fn:fn1">Dominici, M.<em> et al.</em> (2006) Cytotherapy<strong> 8</strong>:315.</li> <li id="fn:fn2">Keating, A. (2012) Cell Stem Cell <strong>10</strong>:709.</li> </ul></body></html> </article> <article class="product-data-wrapper"> <p>The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. However, because these biological properties and hierarchical relationships remain to be clearly demonstrated in vivo, the term 'multipotent mesenchymal stromal cells' is often used to distinguish cultured cells from their in vivo precursors. Originally discovered in mouse bone marrow, multipotent mesenchymal stromal cells cultured from a variety of species and tissue types, have been shown to differentiate into progeny of additional lineages including, cardiomyocytes, endothelial cells, hepatocytes, and neural cells. Again, the physiological relevance of these findings remains to be determined.</p><p></p> </article> </div> </div> <div id="product-specifications" class="nav-link px-0" data-anchor-label="Product Specifications"><table class="tablesaw tablesaw-stack table-bordered table" id="product_specification_wrapper"><h3>Product Specifications for StemXVivo Adipogenic Supplement (100X)</h3><tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Species</td> <td class="product-data-value p-3 px-4 text-break"> Human, Mouse, Rat </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Source</td> <td class="product-data-value p-3 px-4 text-break"> N/A </td> </tr> </table> </div> <div id="scientific-data" class="nav-link px-0" data-anchor-label="Scientific Data"><table class="product-data-wrapper tablesaw tablesaw-stack table" id="data_image_table_wrapper"><h3>Scientific Data Examples for StemXVivo Adipogenic Supplement (100X)</h3><article class="product-data-wrapper"> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/StemXVivo_Adipogenic_Supplement_CCM011_Human_FABP4_ICC_Large_540.png" data-srcset="https://resources.bio-techne.com/images/products/StemXVivo_Adipogenic_Supplement_CCM011_Human_FABP4_ICC_Large_540.png" alt="Detection of FABP4 in Human MSC-differentiated Adipocytes." title="Detection of FABP4 in Human MSC-differentiated Adipocytes." data-caption="Human MSCs were differentiated for 14 days using the Human/Mouse/Rat StemXVivo<sup>®</sup>Osteogenic/Adipogenic Base Media (Catalog # <a class="NoLineLink" href="/p/cell-culture/stemxvivo-osteogenic-adipogenic-base-media_ccm007" target="_self">CCM007</a>) and Human/Mouse/Rat StemXVivo<sup>®</sup>Adipogenic Supplement (Catalog # CCM011). Mature differentiated adipoctyes were detected with a Goat Anti-Human FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # <a class="NoLineLink" href="/p/antibodies/human-fabp4-a-fabp-antibody_af3150" target="_self">AF3150</a>). The cells were stained with a NorthernLights<sup>™</sup>557-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # <a class="NoLineLink" href="/p/secondary-antibodies/goat-igg-northernlights-nl557-conjugated-antibody_nl001" target="_self">NL001</a>; red) and the nuclei were counterstained with DAPI (blue)." data-badges="" data-application="Immunocytochemistry" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <p> <strong>Detection of FABP4 in Human MSC-differentiated Adipocytes.</strong> Human MSCs were differentiated for 14 days using the Human/Mouse/Rat StemXVivo<sup>®</sup>Osteogenic/Adipogenic Base Media (Catalog # <a href="/p/cell-culture/stemxvivo-osteogenic-adipogenic-base-media_ccm007" target="_self">CCM007</a>) and Human/Mouse/Rat StemXVivo<sup>®</sup>Adipogenic Supplement (Catalog # CCM011). Mature differentiated adipoctyes were detected with a Goat Anti-Human FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # <a href="/p/antibodies/human-fabp4-a-fabp-antibody_af3150" target="_self">AF3150</a>). The cells were stained with a NorthernLights<sup>™</sup>557-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # <a href="/p/secondary-antibodies/goat-igg-northernlights-nl557-conjugated-antibody_nl001" target="_self">NL001</a>; red) and the nuclei were counterstained with DAPI (blue). </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/StemXVivo_Adipogenic_Supplement_CCM011_Mouse_FABP4_ICC_Large_540.png" data-srcset="https://resources.bio-techne.com/images/products/StemXVivo_Adipogenic_Supplement_CCM011_Mouse_FABP4_ICC_Large_540.png" alt="Detection of FABP4 in Mouse MSC-differentiated Adipocytes." title="Detection of FABP4 in Mouse MSC-differentiated Adipocytes." data-caption="Mouse MSCs were differentiated for 14 days using the Human/Mouse/Rat StemXVivo<sup>®</sup>Osteogenic/Adipogenic Base Media (Catalog # <a class="NoLineLink" href="/p/cell-culture/stemxvivo-osteogenic-adipogenic-base-media_ccm007" target="_self">CCM007</a>) and Human/Mouse/Rat StemXVivo<sup>®</sup>Adipogenic Supplement (Catalog # CCM011). Mature differentiated adipoctyes were detected with a Goat Anti-Mouse FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # <a class="NoLineLink" href="/p/antibodies/mouse-rat-fabp4-a-fabp-antibody_af1443" target="_self">AF1443</a>). The cells were stained with a NorthernLights<sup>™</sup>557-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # <a class="NoLineLink" href="/p/secondary-antibodies/goat-igg-northernlights-nl557-conjugated-antibody_nl001" target="_self">NL001</a>; red) and the nuclei were counterstained with DAPI (blue)." data-badges="" data-application="Immunocytochemistry" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <p> <strong>Detection of FABP4 in Mouse MSC-differentiated Adipocytes.</strong> Mouse MSCs were differentiated for 14 days using the Human/Mouse/Rat StemXVivo<sup>®</sup>Osteogenic/Adipogenic Base Media (Catalog # <a href="/p/cell-culture/stemxvivo-osteogenic-adipogenic-base-media_ccm007" target="_self">CCM007</a>) and Human/Mouse/Rat StemXVivo<sup>®</sup>Adipogenic Supplement (Catalog # CCM011). Mature differentiated adipoctyes were detected with a Goat Anti-Mouse FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # <a href="/p/antibodies/mouse-rat-fabp4-a-fabp-antibody_af1443" target="_self">AF1443</a>). The cells were stained with a NorthernLights<sup>™</sup>557-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # <a href="/p/secondary-antibodies/goat-igg-northernlights-nl557-conjugated-antibody_nl001" target="_self">NL001</a>; red) and the nuclei were counterstained with DAPI (blue). </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/StemXVivo_Adipogenic_Supplement_CCM011_Rat_FABP4_ICC_Large_540.png" data-srcset="https://resources.bio-techne.com/images/products/StemXVivo_Adipogenic_Supplement_CCM011_Rat_FABP4_ICC_Large_540.png" alt="Detection of FABP4 in Rat MSC-differentiated Adipocytes." title="Detection of FABP4 in Rat MSC-differentiated Adipocytes." data-caption="Rat MSCs were differentiated for 14 days using the Human/Mouse/Rat StemXVivo<sup>®</sup>Osteogenic/Adipogenic Base Media (Catalog # <a class="NoLineLink" href="/p/cell-culture/stemxvivo-osteogenic-adipogenic-base-media_ccm007" target="_self">CCM007</a>) and Human/Mouse/Rat StemXVivo<sup>®</sup>Adipogenic Supplement (Catalog # CCM011). Mature differentiated adipoctyes were detected with a Goat Anti-Mouse FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # <a class="NoLineLink" href="/p/antibodies/mouse-rat-fabp4-a-fabp-antibody_af1443" target="_self">AF1443</a>). The cells were stained with a NorthernLights<sup>™</sup>557-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # <a class="NoLineLink" href="/p/secondary-antibodies/goat-igg-northernlights-nl557-conjugated-antibody_nl001" target="_self">NL001</a>; red) and the nuclei were counterstained with DAPI (blue)." data-badges="" data-application="Immunocytochemistry" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <p> <strong>Detection of FABP4 in Rat MSC-differentiated Adipocytes.</strong> Rat MSCs were differentiated for 14 days using the Human/Mouse/Rat StemXVivo<sup>®</sup>Osteogenic/Adipogenic Base Media (Catalog # <a href="/p/cell-culture/stemxvivo-osteogenic-adipogenic-base-media_ccm007" target="_self">CCM007</a>) and Human/Mouse/Rat StemXVivo<sup>®</sup>Adipogenic Supplement (Catalog # CCM011). Mature differentiated adipoctyes were detected with a Goat Anti-Mouse FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # <a href="/p/antibodies/mouse-rat-fabp4-a-fabp-antibody_af1443" target="_self">AF1443</a>). The cells were stained with a NorthernLights<sup>™</sup>557-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # <a href="/p/secondary-antibodies/goat-igg-northernlights-nl557-conjugated-antibody_nl001" target="_self">NL001</a>; red) and the nuclei were counterstained with DAPI (blue). </p> </td> </tr> </article> </table> </div> <div id="preparation--storage" class="nav-link px-0" data-anchor-label="Preparation & Storage"><div id="cc_prep_storage"><h3>Preparation & Storage</h3><table class="table-bordered table" id="prep_storage_wrapper"><tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Shipping Conditions</td> <td class="product-data-value p-3 px-4 text-break"> The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Storage</td> <td class="product-data-value p-3 px-4 text-break"> Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. </td> </tr> </table> </div> </div> <div id="product-datasheets-anchor" class="nav-link static-anchor p-0" data-anchor-label="Product Datasheets"></div> </div> </div> <div class="tab-pane fade mx-4 py-4 show" id="procedure-tab" data-tab-label="Procedure" role="tabpanel" aria-labelledby="procedure-tab-tab"><div id="cell_culture_assay_procedure"><h3>Assay Procedure</h3><article class="product-data-wrapper"> <html><head><style type="text/css">.psp-procedure {margin:20px 0; clear:both; border-top:1px solid #efefef;} .psp-procedure > div {float:left; width:48%; padding:5px;} .psp-step .clear {clear:both;} </style> </head><body><p><strong>Refer to the product <a href="//resources.rndsystems.com/pdfs/datasheets/CCM011.pdf" target="_blank" class="a___link-external">datasheet</a> for complete product details.</strong></p> <div class="grid_7"> <p>Briefly, human, mouse, or rat MSCs are differentiated into adipocytes using the following in vitro differentiation procedure:</p> <ul> <li>Culture multipotent cells of interest</li> <li>Induce adipogenic differentiation using a media supplement</li> <li>Evaluate differentiation using a mature phenotype marker antibody and fluorescent ICC</li> </ul> <p>For use with Human/Mouse/Rat StemXVivo<sup>®</sup> Osteogenic/Adipogenic Base Media (Catalog # <a href="/p/cell-culture/stemxvivo-osteogenic-adipogenic-base-media_ccm007">CCM007</a>).</p> </div> <div class="grid_4 text_center"><a href="//resources.rndsystems.com/images/site/ccm011-protocol-overlay.png" target="_blank" title="View CCM011 Protocol Graphic Summary" class="a___link-external"><img alt="CCM011 Protocol Graphic Summary" src="//resources.rndsystems.com/images/site/ccm011-protocol-tn.png" width="232"><br> View Graphic Procedure</a></div> <div class="clear"> </div> <p> </p> <div class="ds_lightBlueBar">Reagents Provided</div> <h4>Reagents supplied in the Human/Mouse/Rat StemXVivo<sup>®</sup> Adipogenic Supplement (Catalog # CCM011):</h4> <ul> <li>Human/Mouse/Rat StemXVivo<sup>®</sup> Osteogenic/Adipogenic Base Media (Catalog # <a href="/p/cell-culture/stemxvivo-osteogenic-adipogenic-base-media_ccm007">CCM007</a> or equivalent)</li> <li>Penicillin-Streptomycin-Glutamate (100X)</li> </ul> <p> </p> <div class="ds_lightBlueBar">Other Supplies Required</div> <h4>Reagents</h4> <ul> <li>StemXVivo<sup>®</sup> Osteogenic/Adipogenic Base Media (Catalog # <a href="/p/cell-culture/stemxvivo-osteogenic-adipogenic-base-media_ccm007">CCM007</a> or equivalent)</li> <li>Penicillin-Streptomycin-Glutamate (100X)</li> </ul> <h4>Materials</h4> <ul> <li>MSCs</li> <li>10 cm tissue culture plates</li> <li>15 mL centrifuge tubes</li> <li>Pipettes and pipette tips</li> <li>Serological pipettes</li> </ul> <h4>Equipment</h4> <ul> <li>37 °C and 5% CO<sub>2</sub> incubator</li> <li>Centrifuge</li> <li>Hemocytometer</li> <li>Inverted microscope</li> <li>2 °C to 8 °C refrigerator</li> <li>37 °C water bath</li> </ul> <p> </p> <div class="ds_lightBlueBar">Procedure Overview</div> <p>This protocol has been tested using bone marrow- and/or adipose tissue-derived MSCs. If using a different tissue source or cell line, the protocol below may need to be optimized.</p> <h4><strong>Adipogenic Differentiation</strong></h4> <div class="psp-procedure"> <div class="psp-step"> <p><strong>Plate</strong> 2.1 x 10<sup>4</sup> MSCs/cm<sup>2</sup> in StemXVivo<sup>®</sup> Osteogenic/Adipogenic Base Media.</p> <p><strong>Culture</strong> cells to 100% confluency.</p> </div> <div><img alt="Culture cells to 100% confluency." src="http://resources.rndsystems.com/images/site/ccm011-protocol-step1.png"></div> </div> <div class="psp-procedure"> <div class="psp-step"> <p><strong>Replace</strong> the medium with StemXVivo<sup>®</sup> Adipogenic Differentiation Medium to induce adipogenesis.</p> </div> <div><img alt="Replace the medium with StemXVivo Adipogenic Differentiation Medium to induce adipogenesis." src="http://resources.rndsystems.com/images/site/ccm007-protocol-adipo-step2.png"></div> </div> <div class="psp-procedure"> <div class="psp-step"> <p><strong>Every 3-4 days</strong>, replace with fresh Adipogenic Differentiation Medium.</p> </div> <div> </div> </div> <div class="psp-procedure"> <div class="psp-step"> <p><strong>After 7-21 days</strong>, adipocytes can be harvested and analyzed.</p> </div> <div><img alt="After 7-21 days, adipocytes can be harvested and analyzed." src="http://resources.rndsystems.com/images/site/ccm011-protocol-step3.png"></div> </div></body></html> </article> </div> </div> <div class="tab-pane fade mx-4 py-4 show" id="citations-reviews-faqs-tab" data-tab-label="Citations / Reviews / FAQs" role="tabpanel" aria-labelledby="citations-reviews-faqs-tab-tab"><div class="product_details_anchors mb-2"> <div class="nav mx-0 pt-4 pb-2 m___nav-anchors d-none d-lg-flex"> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#citations"> Citations </a> </li> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#reviews"> Reviews </a> </li> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#faqs"> FAQs </a> </li> </div> </div> <div id="citations"> <section id="citations-tab-content-placeholder" class="tab-content-placeholder" data-id="citations" data-product_code="CCM011"> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> </section> </div> <div class="mt-4" id="reviews"> <h2 class="section-title"> Customer Reviews for StemXVivo Adipogenic Supplement (100X) </h2> <div class="pp_filter_list border-bottom mb-4 pb-3"> <div class="row mb-4 pb-4"> <div class="col-md-6"> <p class="mt-2"> There are currently no reviews for this product. 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