AAV Vector Production | AAV Plasmid Synthesis

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Walking</a></li><br><li><a class='GColor' href='/en/Public/Services/Molecular-Genetics' style='color:#826ec3;font-size:17px'>PCR + Sanger Services</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Bacterial-and-Fungal-Identification' style='color:#585F69;font-size:14px'>Bacterial and Fungal Identification</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Mutation-Analysis' style='color:#585F69;font-size:14px'>Mutation Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/SNP-Genotyping' style='color:#585F69;font-size:14px'>SNP Genotyping</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package' style='color:#585F69;font-size:14px'>PCR Plus Sequencing CRISPR Analysis Package</a></li><li><a href='/en/Public/Services/Molecular-Genetics/cDNA-Verification' style='color:#585F69;font-size:14px'>Confirmatory Sequencing & Cell Bank Characterization</a></li><li><a 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href='/en/Public/Services/Gene-Synthesis/Antibody-DNA-Synthesis' style='color:#585F69;font-size:14px'>Antibody Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/AAV-Plasmid-Synthesis' style='color:#585F69;font-size:14px'>AAV Plasmid Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Fragments' style='color:#585F69;font-size:14px'>Gene Fragments</a></li><li><a href='/en/Public/Services/Gene-Synthesis/CRISPR-Construct-Synthesis' style='color:#585F69;font-size:14px'>CRISPR Construct Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Synthetic-DNA-Libraries' style='color:#585F69;font-size:14px'>Synthetic DNA Libraries</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Single-Stranded-DNA-Synthesis' style='color:#585F69;font-size:14px'>ssDNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/DNA-Cloning' style='color:#585F69;font-size:14px'>DNA Cloning</a></li><li><a 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Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a 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Utilizing our decade-plus experience in synthesizing DNA, GENEWIZ from Azenta Life Sciences has developed a unique adeno-associated virus vector production service, the first of its kind, for researchers working with adeno-associated virus vectors. GENEWIZ AAV Vector Production service, listed as AAV Plasmid Synthesis in our <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://clims4.genewiz.com/GeneSynthesis/BulkOrderForm?lobId=2&serviceId=213" target="_blank">CLIMS4 ordering system</a>, comes bundled with our <a href="/en/Public/Services/Plasmid-DNA-Prep/AAV-Plasmid-Preparation">AAV Plasmid Preparation</a> protocol to deliver mini- to giga-scale AAV plasmids that have been sequence-verified by our <a href="/en/Public/Services/Sanger-Sequencing/AAV-ITR">AAV-ITR Sanger Sequencing service</a>.</p> <p>We understand the importance of intact inverted terminal repeat (ITR) regions in downstream AAV gene therapy applications. GENEWIZ maintains a 2-step QC process and ITR correction by gene synthesis to ensure the integrity of your ITR regions.</p> <p> </p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/gene-synth-inquiry?utm_source=aav-plasmid-synthesis" target="_blank"><button>Place an Order</button></a> </div> <div class="ngs-content-box" id="ngs-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/aav-plasmid-synthesis-f?_ga=2.143383728.487161022.1619428294-1393965845.1616062566&_gac=1.188751065.1618931615.Cj0KCQjw9_mDBhCGARIsAN3PaFPvGTEcX5lMJvigm7xETNDZMYbIxQS8p2Au7CtZojbJy61yV52_39waAjCcEALw_wcB" target="_blank"><img alt="" src="/-/media/Images/GS_AAV-Plasmid_Synthesis_US_CLIMS-Ad.ashx?la=en&hash=FF922CB210CCE5EEFBE8B213E39CE075DA8511B1" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <section id="workflow"> <article class="desktop12"> <div class="container"> <header class="contain marg-sm-btm "> <h2 style="text-align: center;">PROPRIETARY AAV PLASMID SYNTHESIS WORKFLOW</h2> </header> <img alt="" class="desktop12" src="/-/media/Images/ProprietaryAAVPlasmid-Prep_Workflow_1100.ashx?la=en&hash=478BA1DD036BD30C481671EC14568C56388A75F3"> </div> </article> </section> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; 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Regular PCR amplification cannot repair the sequence due to its repetitive nature. Azenta can successfully deliver repaired inverted terminal repeat regions through specific ITR correction technique.</p></header> <img alt="" class="desktop12 padding-top-10 padding-bottom-10" src="/-/media/Images/Services/GS/AAV2021/ITRBeforeandAfterCorrectionSequence1x.ashx?la=en&hash=1CBA00FFE4CF39469B1C256A8CCDAFCE9F2C3BBB"> </div> </article> </section> </div> <div class="headline-center Rich-Text-Edit-Area"> <section id="importance" class=""> <article class="desktop12"> <header class="contain marg-sm-btm "><h2 align="center">IMPORTANCE OF INTACT ITR REGIONS IN AAV GENE THERAPY RESEARCH</h2> <p>Each inverted terminal repeat region is important in downstream AAV gene therapy applications*</p></header> <div class="container"> <div class=" flexbox flex-center flex-column col-centered"> <table cellpadding="0" cellspacing="0" border="0" class=" table table-responsive table-bordered desktop7 tablet12 phone12"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <td class="desktop6">Function</td> <td class="desktop6">Effecting Region</td> </tr> <tr> <td>Residue of Non-Recombinant AAV (r(AAV)) Encapsided DNA</td> <td>A-A’</td> </tr> <tr> <td>Packing</td> <td>A-A’ (trs), D</td> </tr> <tr> <td>Productivity</td> <td>B-B’, C-C’</td> </tr> <tr> <td>Encapsidation</td> <td>A-A’, B-B’, C-C’, D</td> </tr> <tr> <td>Affinity to Rep Protein</td> <td>A-A’, B-B’, C-C’</td> </tr> <tr> <td>Transduction Efficiency</td> <td>A-A’</td> </tr> <tr> <td>Replication</td> <td>A-A’, D</td> </tr> <tr> <td>Rescue</td> <td>A-A’ (trs), D</td> </tr> <tr> <td>Integration</td> <td>A-A’ (trs), D</td> </tr> </tbody> </table> </div> <p class="footnote">* Savy, A., Dickx, Y., Nauwynck, L., Bonnin, D., Merten, O.W. and Galibert, L., 2017. Impact of inverted terminal repeat integrity on rAAV8 production using the baculovirus/Sf9 cells system. Human Gene Therapy Methods, 28(5), pp.277-289. </p> <p class="footnote">*Zhou, Q., Tian, W., Liu, C., Lian, Z., Dong, X. and Wu, X., 2017. Deletion of the B-B’and C-C’regions of inverted terminal repeats reduces rAAV productivity but increases transgene expression. Scientific reports, 7(1), pp.1-13. </p> <p class="footnote">*Maurer, A.C. and Weitzman, M.D., 2020. Adeno-Associated Virus Genome Interactions Important for Vector Production and Transduction. Human Gene Therapy, 31(9-10), pp.499-511. </p> <p class="footnote">*trs – terminal resolution site</p> </div> </article> </section> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/77007-D-0724-Workshops_Medium_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content { text-align: center; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; line-height: 115%; font-size: 40px; margin: 0; padding-bottom: 10px; padding-top: 90px; } .covid-box h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 0; margin-bottom: 5px; } .covid-box p { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 25px; margin: 0; margin-bottom: 5px; } .covid-box button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box button:hover { cursor: pointer; transform: scale(1.1); } .covid-box button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner { width: 100%; height: auto; background-image: url("/-/media/77007-D-0724-Workshops_Mobile_Background3.ashx"); background-size: cover; background-position: center; text-align: center; padding: 2px 2 2px 2; } .covid-content { width: 90%; margin: 0px 0 0px 0; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 350; line-height: 115%; font-size: 35px; margin: 0; padding-bottom: 10px; padding-top: 90px; } .covid-box p { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 250; font-size: 15px; margin: 0; margin-bottom: 5px; } } </style> <br /> <div class="covid-banner"> <div class="covid-content"> <div class="covid-box"> <h3>Enhancing Gene Transfer Efficiency:<br /> Viral vs. Non-Viral Methods</h3> <p>Friday, September 20, 2024 | 11:00AM EDT</p> <a href="https://hubs.ly/Q02JnBCB0" target="_blank"> <button><strong> REGISTER NOW</strong></button> </a> <p> </p> </div> </div> </div> <p> </p> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; } .tech-resources-container { width: 1170px; margin: 0 auto; padding: 20px; } .tech-resources-container h2 { text-align: center; border-bottom: none; } .tech-resource { display: flex; align-items: center; flex-direction: row; margin-bottom: 35px; } .resource { flex-grow: 1; } .resource-right h3, .resource-right p { width: 80%; margin: 0 auto; border-bottom: none; text-align: left; } .resource button { padding: 5px 15px; border: none; border-radius: 4px; background: #826ec3; color: #fff; font-size: 15px; font-weight: lighter; letter-spacing: 1px; margin-top: 15px; -webkit-transition: ease-in-out 0.2s; -moz-transition: ease-in-out 0.2s; -o-transition: ease-in-out 0.2s; transition: ease-in-out 0.2s; } .resource button:hover { background: #6959a1; } @media only screen and (max-width: 800px) { .tech-resource { flex-direction: column; text-align: center; } .resource-right h3, .resource-right p { text-align: center; } } @media only screen and (max-width: 1170px) { .tech-resources-container { width: 80%; margin: 0 auto; } } </style> <div style="width: 100%; background-color: #f0f0f0; padding: 20px; margin: 0;margin-top: 20px;"> <h2>AAV VECTOR PRODUCTION TECHNICAL RESOURCES</h2> <div class="tech-resources-container"> <div class="tech-resource"> <div class="resource resource-left"> <img alt="Tech Note" src="/-/media/Plasmid-Prep-Tech-Note-Thumbnail.ashx?h=160&w=220&la=en&hash=F37CE1AD4FC30DCAB54FD0C2849F373956556CE4" /> </div> <div class="resource resource-right"> <h3>Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins</h3> <p> </p> <p>Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. This tech note explores strategies to optimize bacterial growth conditions, enhance lysis, and refine purification protocols to boost pDNA yield and purity, ensuring consistent, high-quality results.<br /> <a href="https://web.genewiz.com/technote/optimizingplasmidprep" target="_blank"> <button>Download Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <div class="image"> <img alt="Tech Note: An Efficient and Reliable Approach to AAV Packaging" src="/-/media/12051-D_AAV-Packaging_TechResource-Thumb.ashx?h=160&w=220&la=en&hash=B13220BDC7AA1F511A6C578121266C42359E7B9E" /> </div> </div> <div class="resource resource-right"> <h3>Tech Note: An Efficient and Reliable Approach to AAV Packaging</h3> <p> </p> <p>While AAV is a powerful vehicle for gene delivery, unstable ITRs threaten the fidelity of rAAV genomes. Loss of ITR integrity diminishes the packaging efficiency of rAAV particles and thus viral titer. In this tech note, discover the effect of partial and full ITR deletions on AAV packaging and how to reliably identify and effectively correct them.<br /> <a href="https://web.genewiz.com/tech-note/aav-packaging" target="_blank"> <button>Download Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <div id="resources"> <img alt="Tech Note: An Efficient and High-Fidelity Approach to AAV Plasmid Preparation" src="/-/media/Images/AAV_Plasmid_Prep_Tech_Note_Thumbnail.ashx?h=160&w=200&la=en&hash=4FB2021CE3EC4E31C10BD4FD46A140DA83038F2A" /> </div> </div> <div class="resource resource-right"> <h3>Tech Note: An Efficient and High-Fidelity Approach to AAV Plasmid Preparation</h3> <p> </p> <p>Azenta has recently developed a proprietary AAV plasmid preparation process, which significantly improves the chance of maintaining ITR integrity, even when other commercially available competent cells growing in low temperature have failed. This process can also isolate clones with full-length ITR from a sample mixture containing intact and truncated ITRs. <br /> <a href="https://web.genewiz.com/tech-note/aav-plasmid-prep" target="_blank"> <button>Download Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="A Comprehensive Guide to Using AAV Vectors in Gene Therapy" src="/-/media/Images/11019-D_AAV-Workshop_TechResource-Thumbnail.ashx?h=160&w=220&la=en&hash=4BAE0BF24968E76D3FC0D201099442A2BCFD84CD" /> </div> <div class="resource resource-right"> <h3>Workshop & Roundtable Discussion: A Comprehensive Guide to Using AAV Vectors in Gene Therapy</h3> <p> </p> <p>Working with AAV vectors can be challenging. In this on-demand workshop & roundtable discussion, you’ll gain a better understanding of the AAV development pipeline for gene therapy research and learn how to optimize upstream and downstream processes including AAV synthesis, sequencing, bioinformatics, and storage. <br /> <a href="https://web.genewiz.com/webinar/guide-using-aav-vectors-gene-therapy" target="_blank"> <button>Watch Now</button> </a> </p> </div> </div> </div> </div> </div> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".SingleIcon").find("img").addClass("img-responsive center-block"); }); </script> <div class="process-v1 icon-nav Component-BackGround-Default margin-top-10 margin-bottom-0 padding-top-10 padding-bottom-10"> <div class="container"> <div class="headline-center"> <h2>Related Services</h2> <p></p> </div> <ul class="list-unstyled row "> <li class='col-sm-4 services'> <a href='/en/Public/Services/Sanger-Sequencing/AAV-ITR' target=''> <div class='SingleIcon 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