The Clinical Proteomic Technologies for Cancer

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href="javascript:window.print()"><i class="fa fa-print" aria-hidden="true"></i> PRINT</a></div> </div> <h1 id="page-title" class="title">BRCA1 Interacting Protein C-Terminal Helicase 1 Peptide 3</h1> <hr /> </div> <div id="block-system-main" class="block block-system block-system-main" > <div class="content" id="detail_page_content"> <article id="node-27" class="node node-page view-mode-full" about="https://proteomics.cancer.gov/antibody-portal/available-antibodies/antibody-portal" typeof="foaf:Document"> <span property="dc:title" content="Antibody Portal" class="rdf-meta element-hidden"></span> <span property="sioc:num_replies" content="0" datatype="xsd:integer" class="rdf-meta element-hidden"></span> </article>  <div id="search-fields-container_antibody-browse"> <a title="Expand all page details" href="javascript:void(0);" onclick="showAllAntigenDataBlock('open',1)">Expand All</a> - <a title="Collapse all page details" href="javascript:void(0);" onclick="showAllAntigenDataBlock('close',1)">Collapse All</a> </div> <div class="clear"></div> <div id="detail-menu"> <div class="detail-menu-title"><h2>Antibodies</h2></div> <ul> <li class="antibody-name"> <h3><a href="#CPTC-BRIP1-5">CPTC-BRIP1-5</a></h3>  <a class="manageMenuBlock-link" href="javascript:void(0)" id="top-antibody-1" onclick="manageMenuBlock(this.id); showDataBlock('antibody-1',false)">[hide details]</a> <div class="top_links" id="menu-top-antibody-1"> <ul class="sub-menu"> <li><a href="#CPTC-BRIP1-5-Background">Background</a>| <a href="#CPTC-BRIP1-5-Characterization">Characterization Data</a></li> </ul> </div> </li> </ul> <div class="detail-menu-title"><h2>Antigen</h2></div> <ul> <li class="antibody-name"><h3><a href="#BRCA1_Interacting_Protein_C_Terminal_Helicase_1_Peptide_3">BRCA1 Interacting Protein C-Terminal Helicase 1 Peptide 3</a></h3> <a title="hide/show details" class="manageMenuBlock-link" href="javascript:void(0)" id="top-antigen" onclick="manageMenuBlock(this.id); showDataBlock('block-antigen')">[hide details]</a> <div class="top_links" id="menu-top-antigen"> <ul class="sub-menu"> <li><a href="#BRCA1 Interacting Protein C-Terminal Helicase 1 Peptide 3-Background">Background</a>| <a href="#BRCA1 Interacting Protein C-Terminal Helicase 1 Peptide 3-Characterization">Characterization Data</a>| <a href="#BRCA1 Interacting Protein C-Terminal Helicase 1 Peptide 3-SOPs">SOPs</a>| <a href="#BRCA1 Interacting Protein C-Terminal Helicase 1 Peptide 3-Links">Links</a></li> </ul> </div> </li> </ul> </div> <a name="CPTC-BRIP1-5"></a> <div class="well"> <div class="detail-type-header"> <a title="hide/show details" class="manageMenuBlock-link" href="javascript:void(0)" onclick="showDataBlock('antibody-1',true)"> <h4><i class="fa fa-minus icon-antibody-1" aria-hidden="true"></i>Antibody: CPTC-BRIP1-5</h4> </a> </div> <div id="antibody-menu-1" title="Click to display Antibody data" class="block_record_menu_down_icon" onclick="manageMenuBlock('top-antibody-1'); showDataBlock('antibody-1',false)"> </div> <div class="block_record" id="antibody-1"> <a name="CPTC-BRIP1-5-Background"></a> <h5 class="nopadding">Background</h5> <div class="detail-label"><p>Catalog Number:</p></div> <div class="detail-value"><p>CPTC-BRIP1-5</p></div> <div class="clear"></div> <div class="detail-label"><p>RRID:</p></div> <div class="detail-value"><p>AB_2820261</p></div> <div class="clear"></div> <div class="detail-label"><p>Target Antigen:</p></div> <div class="detail-value"><p>BRCA1 Interacting Protein C-Terminal Helicase 1 Peptide 3</p></div> <div class="clear"></div> <div class="detail-label"><p>Isotype:</p></div> <div class="detail-value"><p>IgG</p></div> <div class="clear"></div> <div class="detail-label"><p>Species:</p></div> <div class="detail-value"><p>Rabbit Monoclonal Antibody</p></div> <div class="clear"></div> <div class="detail-label"><p>Last Updated:</p></div> <div class="detail-value"><p>11/21/2023</p></div> <div class="clear"></div> <div class="detail-label"><p>Antigen Recognition(s):</p></div> <div class="detail-value"><p>Peptide, Endogenous, Phosphorylation</p></div> <div class="clear"></div> <div class="detail-label"><p>Thematic Panel(s):</p></div> <div class="detail-value"><p>DNA Damage Response (DDR) Pathway</p></div> <div class="clear"></div> <h5>External Links</h5> <ul class="url-list"> <li class="link"> <strong>CPTAC Assay Portal</strong> <a target="_blank" title="Link to CPTAC-3281 (Phospho)" href="https://assays.cancer.gov/CPTAC-3281"> CPTAC-3281 (Phospho) </a> </li> </ul> <ul class="url-list"> <li class="link"> <strong>CPTAC Assay Portal</strong> <a target="_blank" title="Link to CPTAC-3220" href="https://assays.cancer.gov/CPTAC-3220"> CPTAC-3220 </a> </li> </ul> <h5>Purchase</h5> <ul class="url-list"> <a name="CPTC-BRIP1-5-Links"></a> <li class="cart"> <strong>DSHB</strong> <a href="../cart/Q1BUQy1CUklQMS01"> CPTC-BRIP1-5 </a> </li> </ul> <h5>Publications</h5> <ul class="url-list"> <li class="link"> <strong><i class='fas fa-book-open'></i> </strong> <a title="View publication A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5939939/" target="_blank"> A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage (PMID: 29474155) </a> </li> </ul> <a name="CPTC-BRIP1-5-Characterization"></a> <h5>Characterization Data <a title="Compare characterization data" class="show-for-medium-up" href="#" data-open="compareModal" onclick="showCompare(227);">[Compare Characterization Data]</a></h5> <div id="characterization_results_0"> <div class="image-container"> <a href="#" class="characterization_toggle closed" data-char-toggle="characterization_1"> <h6><i class="fa fa-plus characterization_1" aria-hidden="true"></i>  IHC HPA</h6> </a> <div class="characterization_container" id="characterization_1"> Download <a href='/file/download/antibody_original/9db9858d2ed75b9ca2b250f09c514e13' title='Click to download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)'>This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)</a> (294.1 KB) <h3 class="detail-type-header align-left">CPTC-BRIP1-5 Evaluation by the Human Protein Atlas</h3> <div><p>Result: <strong>Negative</strong></p></div> <p class="detail-type-text">This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)</p><br /> <div class="download-file-container"> <div class="antibody-char-sop-box"> <h3 class="detail-type-header">Characterization SOP Files</h3> <ul class="sop-file-list"> <li> <img style="border: 0px;" align="absmiddle" alt="Download file document type" src="/resources/images/site/application_pdf.gif" />  <a title="Download Antibody Evaluation by Human Protein Atlas (62.6 KB)" href="/file/download/characterization_image_sop/2b9c44e43c5c9761c168c019666b3d8f">Antibody Evaluation by Human Protein Atlas (62.6 KB)</a></li> </ul></div> </div> </div> </div> <div class="image-container"> <a href="#" class="characterization_toggle closed" data-char-toggle="characterization_2"> <h6><i class="fa fa-plus characterization_2" aria-hidden="true"></i>  Immuno-MRM</h6> </a> <div class="characterization_container" id="characterization_2"> <div class="center_align"> <a data-open="characterization_image_2" href="#"> <img class="characterization_image image-characterization" data-full-sized-image="characterization_image_2" data-full-sized-image-path="/file/view/antibody_original/29d47282e56fd822ea2bec96eefb0b21" height="255" width="255" alt="Click to enlarge image Immuno-MRM chromatogram of CPTC-BRIP1-5 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3281) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)" src="/file/view/antibody/4040b9c3510939eb479d650d18839b66" data-width="500" /> </a> <span>Click image to enlarge</span> </div> <div id="characterization_image_2" class="reveal" style="width: 500px;" data-reveal aria-labelledby="firstModalTitle" aria-hidden="true" role="dialog"> <div class="close-characterization-image" title="Click to close the IHC Cell Line Reference Image"> <a class="close-reveal-modal" aria-label="Close" href="#">×</a> </div> <img alt="Immuno-MRM chromatogram of CPTC-BRIP1-5 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3281) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)" src="" /> </div> <h3 class="detail-type-header align-left">CPTC-BRIP1-5 iMRM</h3> <div><p>Result: <strong>Positive</strong></p></div> <p class="detail-type-text">Immuno-MRM chromatogram of CPTC-BRIP1-5 antibody (see CPTAC assay portal for details:<br /> https://assays.cancer.gov/CPTAC-3281)<br /> <br /> Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)</p><br /> <div class="download-file-container"> <div class="antibody-char-sop-box"> <h3 class="detail-type-header">Characterization SOP Files</h3> <ul class="sop-file-list"> <li> <img style="border: 0px;" align="absmiddle" alt="Download file document type" src="/resources/images/site/application_pdf.gif" />  <a title="Download Antibody Evaluation (iMRM) by Paulovich Laboratory at Fred Hutchinson Cancer Center (92.7 KB)" href="/file/download/characterization_image_sop/a9dc7b654f30ad8d91b44574b65dc898">Antibody Evaluation (iMRM) by Paulovich Laboratory at Fred Hutchinson Cancer Center (92.7 KB)</a></li> </ul></div> </div> </div> </div> <div class="image-container"> <a href="#" class="characterization_toggle closed" data-char-toggle="characterization_3"> <h6><i class="fa fa-plus characterization_3" aria-hidden="true"></i>  Immunofluorescence - HPA</h6> </a> <div class="characterization_container" id="characterization_3"> Download <a href='/file/download/antibody_original/a1ebb34a8502edb951911aeb7b339db6' title='Click to download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)'>This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)</a> (294.1 KB) <h3 class="detail-type-header align-left">CPTC-BRIP1-5 Evaluation by the Human Protein Atlas</h3> <div><p>Result: <strong>Negative</strong></p></div> <p class="detail-type-text">This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)</p><br /> <div class="download-file-container"> <div class="antibody-char-sop-box"> <h3 class="detail-type-header">Characterization SOP Files</h3> <ul class="sop-file-list"> <li> <img style="border: 0px;" align="absmiddle" alt="Download file document type" src="/resources/images/site/application_pdf.gif" />  <a title="Download Antibody Evaluation by Human Protein Atlas (62.6 KB)" href="/file/download/characterization_image_sop/2b9c44e43c5c9761c168c019666b3d8f">Antibody Evaluation by Human Protein Atlas (62.6 KB)</a></li> </ul></div> </div> </div> </div> <div class="image-container"> <a href="#" class="characterization_toggle closed" data-char-toggle="characterization_4"> <h6><i class="fa fa-plus characterization_4" aria-hidden="true"></i>  NCI 60 Protein Array</h6> </a> <div class="characterization_container" id="characterization_4"> <div class="center_align"> <a data-open="characterization_image_4" href="#"> <img class="characterization_image image-characterization" data-full-sized-image="characterization_image_4" data-full-sized-image-path="/file/view/antibody_original/507b9bd7b0fa02a7bd003bf18de05066" height="145" width="300" alt="Click to enlarge image Protein Array in which CPTC-BRIP1-5 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red)." src="/file/view/antibody/e7a1eebd490519c132996bf4724eeb05" data-width="1250" /> </a> <span>Click image to enlarge</span> </div> <div id="characterization_image_4" class="reveal" style="width: 2361px;" data-reveal aria-labelledby="firstModalTitle" aria-hidden="true" role="dialog"> <div class="close-characterization-image" title="Click to close the IHC Cell Line Reference Image"> <a class="close-reveal-modal" aria-label="Close" href="#">×</a> </div> <img alt="Protein Array in which CPTC-BRIP1-5 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red)." src="" /> </div> <h3 class="detail-type-header align-left">CPTC-BRIP1-5 NCI 60 Protein Array</h3> <div><p>Result: <strong>Positive</strong></p></div> <p class="detail-type-text">Protein Array in which CPTC-BRIP1-5 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).</p><br /> <div class="download-file-container"> <div class="antibody-char-sop-box"> <h3 class="detail-type-header">Characterization SOP Files</h3> <ul class="sop-file-list"> <li> <img style="border: 0px;" align="absmiddle" alt="Download file document type" src="/resources/images/site/application_pdf.gif" />  <a title="Download Well base Reverse Phase Protein Array (255.5 KB)" href="/file/download/characterization_image_sop/70504d3791f343e723340a182bc244e0">Well base Reverse Phase Protein Array (255.5 KB)</a></li> </ul></div> </div> </div> </div> <div class="image-container"> <a href="#" class="characterization_toggle closed" data-char-toggle="characterization_5"> <h6><i class="fa fa-plus characterization_5" aria-hidden="true"></i>  Western Blot - Tissue or Cell Lysate</h6> </a> <div class="characterization_container" id="characterization_5"> <div class="center_align"> <a data-open="characterization_image_5" href="#"> <img class="characterization_image image-characterization" data-full-sized-image="characterization_image_5" data-full-sized-image-path="/file/view/antibody_original/a56569f85fac3edbdd3a2d14ebba77d7" height="300" width="294" alt="Click to enlarge image Western blot using CPTC-BRIP1-5 as primary antibody against HT-29 (lane 2), HeLa (lane 3), MCF7 (lane 4), HL-60 (lane 5), Hep G2 (lane 6), and MCF7 (lane 7) whole cell lysates. Expected molecular weight - 141 kDa and 112 kDa. Molecular weight standards are also included (lane 1)." src="/file/view/antibody/f1c71a1b81b88a4e56d93110a3222e51" data-width="835" /> </a> <span>Click image to enlarge</span> </div> <div id="characterization_image_5" class="reveal" style="width: 835px;" data-reveal aria-labelledby="firstModalTitle" aria-hidden="true" role="dialog"> <div class="close-characterization-image" title="Click to close the IHC Cell Line Reference Image"> <a class="close-reveal-modal" aria-label="Close" href="#">×</a> </div> <img alt="Western blot using CPTC-BRIP1-5 as primary antibody against HT-29 (lane 2), HeLa (lane 3), MCF7 (lane 4), HL-60 (lane 5), Hep G2 (lane 6), and MCF7 (lane 7) whole cell lysates. Expected molecular weight - 141 kDa and 112 kDa. Molecular weight standards are also included (lane 1)." src="" /> </div> <h3 class="detail-type-header align-left">CPTC-BRIP1-5 Western Blot </h3> <div><p>Result: <strong>Negative</strong></p></div> <p class="detail-type-text">Western blot using CPTC-BRIP1-5 as primary antibody against HT-29 (lane 2), HeLa (lane 3), MCF7 (lane 4), HL-60 (lane 5), Hep G2 (lane 6), and MCF7 (lane 7) whole cell lysates. Expected molecular weight - 141 kDa and 112 kDa. Molecular weight standards are also included (lane 1).</p><br /> <div class="download-file-container"> <div class="antibody-char-sop-box"> <h3 class="detail-type-header">Characterization SOP Files</h3> <ul class="sop-file-list"> <li> <img style="border: 0px;" align="absmiddle" alt="Download file document type" src="/resources/images/site/application_pdf.gif" />  <a title="Download Evaluation using Western Blot (262.3 KB)" href="/file/download/characterization_image_sop/10629af866514826ad28b9cb62c78ee5">Evaluation using Western Blot (262.3 KB)</a></li> </ul></div> </div> </div> </div> </div> <div class="top-link"><a title="Back to top of page" href="#top">[top]</a></div> <a name="CPTC-BRIP1-5-SOPs"></a> <div class="clear"></div> </div> </div> <a name="BRCA1_Interacting_Protein_C_Terminal_Helicase_1_Peptide_3"></a> <div class="well"> <div class="detail-type-header"> <a title="hide/show details" class="manageMenuBlock-link" href="javascript:void(0)" onclick="showDataBlock('block-antigen',true)"> <h4><i class="fa fa-minus icon-block-antigen" aria-hidden="true"></i>Antigen: BRCA1 Interacting Protein C-Terminal Helicase 1 Peptide 3</h4> </a> </div> <div id="antigen-menu-down" title="Click to display Antigen data" class="block_record_menu_down_icon" onclick="manageMenuBlock('top-antigen'); showDataBlock('block-antigen',false)"> </div> <div class="block_record" id="block-antigen"> <a name="BRCA1 Interacting Protein C-Terminal Helicase 1 Peptide 3-Background"></a> <h5 class="nopadding">Background</h5> <p> <div class="detail-label"><p>NCI Identification Number:</p></div> <div class="detail-value"><p>00231</p></div> <div class="clear"></div> <div class="detail-label"><p>Antigen Name:</p></div> <div class="detail-value"><p>BRCA1 Interacting Protein C-Terminal Helicase 1 Peptide 3</p></div> <div class="clear"></div> <div class="detail-label"><p>CPTC Name:</p></div> <div class="detail-value"><p>CPTC-BRIP1 Peptide 3</p></div> <div class="clear"></div> <div class="detail-label"><p>Aliases:</p></div> <div class="detail-value"><p>BRCA1 Interacting Protein C-Terminal Helicase 1; BACH1; FANCJ; BRCA1/BRCA2-Associated Helicase 1; ATP-Dependent RNA Helicase BRIP1; BRCA1-Associated C-Terminal Helicase 1; OF; BRCA1-Binding Helicase-Like Protein; Fanconi Anemia Group J Protein; EC 3.6.4.13; BRCA1-Interacting Protein 1; RCA1-Interacting Protein C-Terminal Helicase 1; Protein FACJ; EC 3.6.1</p></div> <div class="clear"></div> <div class="detail-label"><p>Function:</p></div> <div class="detail-value"><p>The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations.<br /> <br /> BRIP1 (BRCA1 interacting protein C-terminal helicase 1) is a protein-coding gene. Diseases associated with BRIP1 include brip1-related fanconi anemia, and brip1-related breast cancer. GO annotations related to this gene include ATP-dependent DNA helicase activity and 4 iron, 4 sulfur cluster binding. An important paralog of this gene is DDX11.<br /> <br /> DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1.</p></div> <div class="clear"></div> <div class="detail-label"><p>Chromosomal Localization:</p></div> <div class="detail-value"><p>17q22.2</p></div> <div class="clear"></div> <div class="detail-label"><p>Accession Number:</p></div> <div class="detail-value"><p>NP_114432.2 </p></div> <div class="clear"></div> <div class="detail-label"><p>UniProt Accession Number:</p></div> <div class="detail-value"><p>Q9BX63</p></div> <div class="clear"></div> <div class="detail-label"><p>DNA Source:</p></div> <div class="detail-value"><p>N/A</p></div> <div class="clear"></div> <div class="detail-label"><p>Immunogen:</p></div> <div class="detail-value"><p>Synthetic Peptide</p></div> <div class="clear"></div> <div class="detail-label"><p>Vector Name:</p></div> <div class="detail-value"><p>N/A</p></div> <div class="clear"></div> <div class="detail-label"><p>Extinction Coefficient:</p></div> <div class="detail-value"><p></p></div> <div class="clear"></div> <div class="detail-label"><p>Buffers:</p></div> <div class="detail-value"><p></p></div> <div class="clear"></div> <div class="detail-label sequence"><p>Expressed Sequence:</p></div> <div class="detail-value sequence"> <p><span class="format-sequence">YSTPPYLLEAASHL(pS)PENFVEDEAK (pS930))</span></p></div> <div class="clear"></div> <div class="detail-label sequence"><p>Native Sequence:</p></div> <div class="detail-value sequence"><p><span class="format-sequence"></span></p></div> <div class="clear"></div> <div class="detail-label"><p>Calculated Isoelectric Point:</p></div> <div class="detail-value"><p>0</p></div> <div class="clear"></div> <div class="detail-label"><p>Molecular Weight:</p></div> <div class="detail-value"><p>2750</p></div> <div class="clear"></div> <div class="detail-label"><p>Last Updated:</p></div> <div class="detail-value"><p>07/20/2017</p></div> <div class="clear"></div> </p> <a name="BRCA1 Interacting Protein C-Terminal Helicase 1 Peptide 3-Links"></a> <h3>Links</h3> <p> <ul class="antigen-url-list"> <li> <strong> EnsEMBL </strong> - <a title="View EnsEMBL link in new window" href="http://www.ensembl.org/Homo_sapiens/Gene/Summary?g=ENSG00000136492;r=17:61681266-61863521" target="_blank">EnsEMBL BRIP1</a> </li> <li> <strong> Entrez </strong> - <a title="View Entrez link in new window" href="https://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=full_report&list_uids=83990" target="_blank">Entrez BRIP1</a> </li> <li> <strong> Uniprot </strong> - <a title="View link in new window" href="https://www.uniprot.org/uniprot/Q9BX63" target="_blank">Uniprot Q9BX63</a> </li> </ul> </p> <a name="BRCA1 Interacting Protein C-Terminal Helicase 1 Peptide 3-Characterization"></a> <h5>Characterization Data</h5> <a name="BRCA1 Interacting Protein C-Terminal Helicase 1 Peptide 3-SOPs"></a> <h3>SOPs</h3> <div class="download-file-container"> <p>No SOPs available.</p> <div class="top-link"><a title="Back to top of page" href="#top">[top]</a></div> </div> </div> </div> <div class="clear"></div> <div id="compareModal" class="reveal" data-reveal aria-labelledby="Compare Antibody Details" aria-hidden="true" role="dialog"> <div id="antibody_compare_details"></div> </div> <input id="mouseX" type="hidden" value="0" /> <input id="mouseY" type="hidden" value="0" /> <div id="compare-original-images"></div>  </div> </div> </div>  <div id="adobe-required"> <h3>Don't have Adobe Reader™? 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The Clinical Proteomic Technologies for Cancer | Details for CPTC-BRIP1-5