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'' : value; }, getCookie: function (key) { var result = ""; var cookies = document.cookie ? document.cookie.split('; ') : []; for (var i = 0; i < cookies.length; i++) { var parts = cookies[i].split('='); var name = decodeURIComponent(parts.shift()); var cookie = parts ? parts.join('=') : []; // Prevent storing or parsing of a cookie that we couldn't decode. if (cookie && key === name) { // removes surrounding quotes if (cookie.indexOf('"') === 0) { // This is a quoted cookie as according to RFC2068, unescape... cookie = cookie.slice(1, -1).replace(/\\"/g, '"').replace(/\\\\/g, '\\'); } // parse utf8 characters try { cookie = decodeURIComponent(cookie); } catch (e) {} result = cookie; // we found it, don't bother looking at other cookies break; } } return result; }, setCookie : function(name, value, numDays){ if ( !numDays ) numDays = 31; var expires = new Date(); expires.setTime(expires.getTime() + (1000 * 60 * 60 * 24 * numDays)); document.cookie = name+ '=' + escape(value) + '; path=/; expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); }, // generic function to set page attributes setPageAttribute: function (key, value) { this.page[key] = value; }, // display digitalData object displayDigitalData: function () { }, // specific function to add an object to digitalData addDigitDataObject: function (key, object) { this[key] = object; }, addChildObjectAttribute: function(child, key, value){ this[child][key] = value; }, initUserData: function(){ var gigyaCookie = this.getCookie("identity_uid"); this.user.gigyaId = ""; this.user.loginStatus = "anonymous"; if( gigyaCookie != ""){ this.user.gigyaId = gigyaCookie; this.user.loginStatus = "logged in"; } }, /** * Check if init has been initiated once * @type {Boolean} */ merchandisingDataInitted: false, initMerchandisingData: function () { // read cid and icid value from session cookie this.merchandising.icid = this.getCookie('s_ev21_tc'); this.merchandising.cid = this.getCookie('s_cmp_tc'); // EMID value this.merchandising.emid = this.getQueryParam("emid"); // referrer by domain if (typeof document.referrer !== 'undefined' && document.referrer.length > 0) { var masterDomain = this.getMasterDomain(); 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document.dispatchEvent(customClickEvent); } } } })(); window.digitalData.init(); </script>  <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_25"); </script> <script type="text/javascript"> var localizedStrings = { "ACCEPTANCE": "Acceptance of Terms of Use", "BTN_AGREE": "Agree", "BTN_DISAGREE": "Disagree", "PROCESSING_MESSAGE" :"processing ... please wait ...", "ADD_TO_CART_QUANTITY_ERROR":"You must provide a quantity to add an item to the cart", "ADD_TO_CART_VALID_QUANTITY_ERROR":"You must provide a valid quantity to add an item to the cart", "VIEW_CART" :"View cart & checkout", "VIEW_CONTACT_DETAIL" :"View Contact Details", "ADD_TO_CART_NOTIFICATION":"Add to Cart Notification", "UPDATE_CART" :"Update Cart", "PRINCIPAL_INVESTIGATOR":"Principal Investigator", "PRINCIPAL_INVESTIGATOR_TEAM_LEADER":"Principal Investigator or Team Leader", "PRINCIPAL_INVESTIGATOR_CHIEF":"Principal Investigator or Chief", "EMAIL_ERROR_1" :"Please Provide Collegues Email", "EMAIL_ERROR_2" :"Please enter a valid colleague email address", "EMAIL_ERROR_3" :"Please Provide Your Email", "EMAIL_ERROR_4" :"Please enter a valid email address", "ERROR_OCCURED_MESSAGE" :"The following errors occured", "NEXT" :"Next", "PREVIOUS" :"Prev", "IMAGE" :"Image", "SAVE_TO_LIST_ERROR_1" :"Please enter a list name.", "SAVE_TO_LIST_ERROR_2" :"Please enter a different list name that does not include the following: ?,/,\\,%,^,!", "MINICART_ERROR_1" :"Unable to connect to eCommerce system. 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role="main"><div class="MainParsys parsys"><div class="text parbase section"><p>Immunoprecipitation (IP) is a cellular and molecular biology method used in laboratories to isolate or purify a specific antigen (or protein of interest) via a solid phase (agarose or Sepharose™ resin or magnetic particles). The IP method was first performed on small aliquots of agarose resin in microcentrifuge tubes. Use of magnetic beads is one method of isolating your protein of interest via IP. Dynabeads, DynaGreen, and Pierce magnetic beads for IP offer exceptional balance of capacity/yield, reproducibility, purity, and cost for smaller-scale isolation of specific proteins (e.g., IP) and protein complexes (<a></a><a href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/protein-interaction-analysis/co-immunoprecipitation-co-ip-pull-down.html">co-IP</a>).</p></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="well-cmp"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><div class="h2"> Selection guide for Dynabeads products </div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><p>Dynabeads products are available for a multitude of molecular and cellular applications. Don’t know where to start? Use our easy selection guide that considers sample type, target molecule, and downstream application to provide tailored recommendations for Dynabeads products.</p><p><a class="cta-link" href="/sg/en/home/brands/product-brand/dynal/dynabeads-magnetic-separation-products-selection-guide.html">Dynabeads product selection guide</a></p></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-fd598175-dace-4391-9fef-15325671dabcMainParsyswell_8fa1uiparimage_44ab"><a href="/sg/en/home/brands/product-brand/dynal/dynabeads-magnetic-separation-products-selection-guide.html"><picture><source srcset='https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.320.high.jpg/1710339445247.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.480.high.jpg/1710339445247.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.full.high.jpg/1710339445247.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.320.high.jpg/1710339445247.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.480.high.jpg/1710339445247.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.320.medium.jpg/1710339445247.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.620.high.jpg/1710339445247.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.320.low.jpg/1710339445247.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.480.medium.jpg/1710339445247.jpg" alt='Conceptual magnetic beads, antibodies, and DNA' title='Dynabeads Products Selection Guide'></picture><style> #cq-image-jsp-fd598175-dace-4391-9fef-15325671dabcMainParsyswell_8fa1uiparimage_44ab img{ width:100%; max-width: 525px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"fd598175-dace-4391-9fef-15325671dabcMainParsyswell_8fa1uiparimage_44ab .image-contain" + "," + "#cq-image-jsp-"+"fd598175-dace-4391-9fef-15325671dabcMainParsyswell_8fa1uiparimage_44ab .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div><div class="well-cmp section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3> On this page: </h3></div><div class="text parbase section"><ul><li><a href="#what">What is immunoprecipitation?</a><ul><li><a href="#how">How does immunoprecipitation work?</a></li><li><a href="#why">Why are magnetic beads used for immunoprecipitation?</a></li></ul></li><li><a href="#selection-guide">Immunoprecipitation beads selection guide</a></li><li><a href="?open=videos#videos">Videos on immunoprecipitation</a></li><li><a href="#automate-sample-preparation">Immunoprecipitation automation protocols</a></li><li><a href="?open=faqs#faqs">Immunoprecipitation FAQs</a></li><li><a href="?open=publications#publications">Immunoprecipitation publications using Dynabeads magnetic beads</a></li><li><a href="#resources">Resources for immunoprecipitation</a></li><li><a href="#order">Order immunoprecipitation beads</a></li></ul></div></div></div></div></div></div><div class="heading section"><h2 id="what"><br>What is immunoprecipitation? </h2></div><div class="text parbase section"><p>Immunoprecipitation (IP) is a cellular and molecular biology method used in laboratories to isolate or purify a specific antigen (or protein of interest) via a solid phase (agarose or Sepharose™ resin or magnetic particles). The IP method was first performed on small aliquots of agarose resin in microcentrifuge tubes. The IP method has evolved to use superparamagnetic particles to specifically target proteins of interest.</p><p><a class="cta-link" href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/immunoprecipitation-myths.html">View common IP myths and tips</a></p></div><div class="heading section"><h3 id="how"> How does immunoprecipitation work? </h3></div><div class="text parbase section"><p>The Dynabeads magnetic beads IP procedure is simple, fast, requires no preclearing step, and results in high-target protein purity, yield, and consistent results. Both DynaGreen magnetic beads and Pierce magnetic beads can perform the same steps with slightly longer protocol times to complete. DynaGreen protocol finishes in <80min. Pierce protocol finishes in <120min.</p></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div container-id="cq-colctrl-lt7" comp-id="A1"><div class="heading section"><h4> Step 1: Binding of the antibody to the beads </h4></div></div><div container-id="cq-colctrl-lt7" comp-id="A2"><div class="text parbase section"><p>Dynabeads, DynaGreen, and Pierce magnetic beads come pre-coated with protein A, protein G, anti-mouse IgG, or anti-rabbit IgG antibodies. Their rapid kinetics allow them to bind to the added antibody in about ten minutes (<b>Figure 1</b>). Biotinylated antibodies can also be used in combination with streptavidin-coupled Dynabeads and Pierce magnetic beads. After antibody incubation a wash step is performed to remove unbound antibodies.</p></div></div><div container-id="cq-colctrl-lt7" comp-id="A3"><div class="heading section"><h4> Step 2: Add sample to the beads </h4></div></div><div container-id="cq-colctrl-lt7" comp-id="A4"><div class="text parbase section"><p>The protein-containing sample is added to the beads-antibody mix and incubated for ten minutes to allow target protein binding. If the protein is of low abundance or low affinity, the incubation time can be increased to one hour or overnight. For most cases, a ten-minute incubation is sufficient.</p></div></div><div container-id="cq-colctrl-lt7" comp-id="A5"><div class="heading section"><h4> Step 3: Wash the protein-bound beads </h4></div></div><div container-id="cq-colctrl-lt7" comp-id="A6"><div class="text parbase section"><p>To help ensure high purity of the bound target protein, the beads should be washed 2 to 4 times with buffer on the magnet to remove all unbound proteins. This step in the isolation process helps ensure a high signal-to-noise ratio.</p></div></div><div container-id="cq-colctrl-lt7" comp-id="A7"><div class="heading section"><h4> Step 4: Elute the protein </h4></div></div><div container-id="cq-colctrl-lt7" comp-id="A8"><div class="text parbase section"><p>The protein can be eluted off the beads by using either a mild elution procedure or a denaturing elution procedure (<b>Figure 2</b>).</p></div></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div container-id="cq-colctrl-lt7" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-2b42f833-67f6-4f02-bda4-fa8bb0d55c1eMainParsysimage_6b4c"><a class="image-enlarge-link" data-lightbox="image_6b4c" href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/_jcr_content/MainParsys/image_6b4c/backgroundimg.img.jpg/1725553119838.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/image_6b4c/foregroundimg.img.320.medium.jpg/1725553119816.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/image_6b4c/foregroundimg.img.480.high.jpg/1725553119816.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/image_6b4c/foregroundimg.img.620.high.jpg/1725553119816.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/image_6b4c/foregroundimg.img.full.high.jpg/1725553119816.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/image_6b4c/foregroundimg.img.320.high.jpg/1725553119816.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/image_6b4c/foregroundimg.img.480.high.jpg/1725553119816.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/image_6b4c/foregroundimg.img.320.medium.jpg/1725553119816.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/image_6b4c/foregroundimg.img.620.high.jpg/1725553119816.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/image_6b4c/foregroundimg.img.320.low.jpg/1725553119816.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/image_6b4c/foregroundimg.img.480.medium.jpg/1725553119816.jpg" alt='Diagram illustrating the steps of immunoprecipitation using Dynabeads magnetic beads: 1) antibody is added to Dynabeads magnetic beads for 10 mins, 2) sample is added to mix for 10 mins, 3) wash steps, and 4) elution' title='Immunoprecipitation with magnetic beads protocol'></picture><style> #cq-image-jsp-2b42f833-67f6-4f02-bda4-fa8bb0d55c1eMainParsysimage_6b4c img{ width:100%; max-width: 700px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"2b42f833-67f6-4f02-bda4-fa8bb0d55c1eMainParsysimage_6b4c .image-contain" + "," + "#cq-image-jsp-"+"2b42f833-67f6-4f02-bda4-fa8bb0d55c1eMainParsysimage_6b4c .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div><div container-id="cq-colctrl-lt7" comp-id="B2"><div class="text parbase section"><small><b>Figure 1. Immunoprecipitation protocol with magnetic beads.</b></small></div></div></div></div><div style="clear:both"></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt8"><div class="parsys_column cq-colctrl-lt8-c0"><div container-id="cq-colctrl-lt8" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-2b42f833-67f6-4f02-bda4-fa8bb0d55c1eMainParsysimage_8ede"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/image_8ede/foregroundimg.img.full.high.png/1725553119867.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/image_8ede/foregroundimg.img.full.high.png/1725553119867.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/image_8ede/foregroundimg.img.320.high.png/1725553119867.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/image_8ede/foregroundimg.img.480.high.png/1725553119867.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/image_8ede/foregroundimg.img.320.medium.png/1725553119867.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/image_8ede/foregroundimg.img.620.high.png/1725553119867.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/image_8ede/foregroundimg.img.320.low.png/1725553119867.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/image_8ede/foregroundimg.img.480.medium.png/1725553119867.png" alt='Chart demonstrating denaturing elution is best used during SDS-PAGE; mild elution is best used for protein characterization, immunization, enzyme studies, amino acid sequence determination, and crystallization; and no elution is needed when performing protein-protein interactions studies, enzyme studies, bioassays, and immunoassays' title='Types of elution buffers for immunoprecipitation with magnetic beads'></picture><style> #cq-image-jsp-2b42f833-67f6-4f02-bda4-fa8bb0d55c1eMainParsysimage_8ede img{ width:100%; max-width: 319px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"2b42f833-67f6-4f02-bda4-fa8bb0d55c1eMainParsysimage_8ede .image-contain" + "," + "#cq-image-jsp-"+"2b42f833-67f6-4f02-bda4-fa8bb0d55c1eMainParsysimage_8ede .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div container-id="cq-colctrl-lt8" comp-id="B1"><div class="text parbase section"><small><b>Figure 2. Types of elution buffers for immunoprecipitation with magnetic beads.</b> Depending on your target protein and how they bind to the magnetic beads, there are three main types of elution buffers to unbind the target protein. Denaturing elution buffers are used for SDS-PAGE. Mild elution buffers are used for protein characterization, immunization, enzyme studies, amino acid sequence determination, and crystallization. Protein-protein interactions, enzyme studies, bioassays, and immunoassays may be performed without eluting your target protein from the magnetic beads.</small></div></div></div></div><div style="clear:both"></div><div class="heading section"><h3 id="why"> Why are magnetic beads used in immunoprecipitation? </h3></div><div class="text parbase section"><p>Magnetic beads for IP have become more common, especially compared to the traditional method of using agarose slurries or resin for protein isolation. This is because magnetic immunoprecipitation beads provide several advantages over the traditional method, such as:</p><ul><li><b>Low background</b>—Little to no non-specific binding and no preclearing required for high-purity protein yields</li><li><b>Highly reproducible</b>—Uniform composition of the magnetic beads ensures the most consistent results</li><li><b>Highly sensitive</b>—<a href="?open=publications#publications">Most-cited method</a> for sensitive applications, such as ChIP and IP for low- abundance proteins</li><li><b>Fast and easy</b>—<40 min protocol with no centrifugation or preclearing steps</li><li><b>Antibody savings</b>—All binding occurs on the smooth outer surface of the beads, which conserves precious antibodies compared to porous agarose beads; using beads for IP is a cost-efficient solution per sample</li><li><b>Automation compatible</b>—Immunoprecipitation beads protocol can be automated on the <a href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/automated-immunoprecipitation-protocols.html">Thermo Fisher Scientific KingFisher platform</a>  </li></ul><p><a class="cta-link" href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/immunoprecipitation-myths.html">View common IP myths on agarose resins vs magnetic beads</a><br><a class="cta-link" href="/sg/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/co-immunoprecipitation-co-ip.html">Learn about co-immunoprecipitation</a></p></div><div class="heading section"><h2 id="selection-guide"><br>Immunoprecipitation beads selection guide </h2></div><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="text parbase section"><ul><li><b><a href="#ab-beads">Antibody-binding magnetic beads</a></b>—Most common method</li><li><b><a href="#surface-beads">Fusion tag-binding magnetic beads</a></b>—If your protein is a recombinant protein</li><li><b><a href="#strep-beads">Biotin-binding magnetic beads</a></b>—If your protein is biotinylated</li><li><b><a href="#surf-beads">Surface-activated magnetic beads</a></b>—If your ligand or target sample is tricky</li></ul></div></div></div></div></div></div><div class="heading section"><h3 id="ab-beads"> Antibody-binding magnetic beads for IP </h3></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-418319010"><tbody><tr><th> </th><th>Protein A, G, A/G</th><th>Secondary antibodies<br><b></b>(anti-mouse, anti-rabbit)</th></tr><tr><td><b>Recommended products</b></td><td><b>Beads only:</b><ul><li><a href="/order/catalog/product/10001D">Dynabeads Protein A</a> </li><li><a href="/order/catalog/product/10003D">Dynabeads Protein G</a> </li><li><a href="/order/catalog/product/78609">Pierce Protein A/G</a> </li><li><a href="/order/catalog/product/80101G">DynaGreen Protein A</a> </li><li><a href="/order/catalog/product/80104G">DynaGreen Protein A/G</a></li></ul><b>Kits:</b><ul><li><a href="/order/catalog/product/10006D">Dynabeads Protein A</a> </li><li><a href="/order/catalog/product/10007D">Dynabeads Protein G</a> </li><li><a href="/order/catalog/product/88804">Pierce Protein A/G</a> </li><li><a href="/order/catalog/product/88805">Pierce Crosslink Kit (A/G)</a></li></ul></td><td><b>Beads only:</b><ul><li><a href="/order/catalog/product/11201D">Dynabeads M-280, Sheep-anti Mouse IgG</a> </li><li><a href="/order/catalog/product/11203D">Dynabeads M-280, Sheep-anti Rabbit IgG</a></li><li><a href="/order/catalog/product/80108G">DynaGreen CaptureSelect Anti-IgG-Fc  (multispecies)</a></li></ul></td></tr><tr><td valign="top"><b>Binding properties</b></td><td colspan="2" style="text-align: center;">Non-covalent antibody binding</td></tr><tr><td valign="top"><b>Antibody co-eluted off the beads</b></td><td>Yes*<br><a href="/order/catalog/product/88805">No (Pierce Crosslink Magnetic IP/Co-IP Kit)</a></td><td>Yes*</td></tr><tr><td width="24%" valign="top"><b>Type of ligand</b></td><td>Different ligands bind different species and antibody subclasses with different specificity<sup>†</sup></td><td><ul><li>Anti-mouse binds mouse IgG1, IgG2a, IgG2b</li><li>Anti-rabbit binds all rabbit IgGs</li></ul></td></tr><tr><td width="24%" valign="top"><b>Mass spec compatible</b></td><td>Yes<br><ul><li>DynaGreen Protein A/G</li><li>DynaGreen Protein A</li><li>DynaGreen CaptureSelect Anti-IgG-Fc (multispecies)</li><li>Pierce MS-Compatible Magnetic IP Kit, </li><li>Pierce Protein A/G</li></ul> Not validated<br><ul><li>Dynabeads Protein A</li><li>Dynabeads Protein G </li></ul></td><td>Yes<br> No (Dynabeads M-280)</td></tr><tr><td width="24%" valign="top"><b>Non-specific binding</b></td><td colspan="2" style="text-align: center;">Low</td></tr></tbody></table></div><p>* Crosslinking can be performed to avoid co-elution of the antibody, but this can decrease the yield of the target antigen.<br> † Learn which <a href="/sg/en/home/life-science/antibodies/antibodies-learning-center/antibodies-resource-library/antibody-methods/comparison-antibody-igg-binding-proteins.html">antibody-binding proteins</a> are best for your IP antibody.</p></div><div class="accordion section"><div class="accordion" id="4bf230458f674938b70c9b3d9345d1bf" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#4bf230458f674938b70c9b3d9345d1bf" href="#1"><span class="caret"></span> Benchmarking data: Dynabeads magnetic beads vs other IP methods </a></div><div class="accordion-body collapse " id="1"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="heading section"><div class="h2"> Dynabeads magnetic beads compared to Sepharose- and agarose-based solutions </div></div><div class="image parbase section"><div id="cq-image-jsp-2b42f833-67f6-4f02-bda4-fa8bb0d55c1eMainParsysaccordion_9be8itemsparaccordionitem_1404itemParsysimage_471f"><a class="image-enlarge-link" data-lightbox="image_471f" href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/_jcr_content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_471f/backgroundimg.img.jpg/1725553120089.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_471f/foregroundimg.img.320.low.jpg/1725553120066.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_471f/foregroundimg.img.320.medium.jpg/1725553120066.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_471f/foregroundimg.img.480.medium.jpg/1725553120066.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_471f/foregroundimg.img.620.high.jpg/1725553120066.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_471f/foregroundimg.img.full.high.jpg/1725553120066.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_471f/foregroundimg.img.full.high.jpg/1725553120066.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_471f/foregroundimg.img.320.high.jpg/1725553120066.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_471f/foregroundimg.img.480.high.jpg/1725553120066.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_471f/foregroundimg.img.320.medium.jpg/1725553120066.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_471f/foregroundimg.img.620.high.jpg/1725553120066.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_471f/foregroundimg.img.320.low.jpg/1725553120066.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_471f/foregroundimg.img.480.medium.jpg/1725553120066.jpg" alt='Two western blots showing the best protein yield and shortest protocol time is precipitated using Dynabeads Protein G magnetic beads compared to Sepharose spin column or slurry, agarose slurry and spin column, resin spin column, and pure protein Protein G ' title='Dynabeads magnetic beads compared to Sepharose- and agarose-based solutions'></picture><style> #cq-image-jsp-2b42f833-67f6-4f02-bda4-fa8bb0d55c1eMainParsysaccordion_9be8itemsparaccordionitem_1404itemParsysimage_471f img{ width:100%; max-width: 1613px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"2b42f833-67f6-4f02-bda4-fa8bb0d55c1eMainParsysaccordion_9be8itemsparaccordionitem_1404itemParsysimage_471f .image-contain" + "," + "#cq-image-jsp-"+"2b42f833-67f6-4f02-bda4-fa8bb0d55c1eMainParsysaccordion_9be8itemsparaccordionitem_1404itemParsysimage_471f .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div><div class="text parbase section"><p><b>Figure 3. Shorter protocol time and better target protein yields with Dynabeads magnetic beads.</b> Equal amounts of sample material with respect to Ab content and cell lysate volume were used for all IP protocols according to the manufacturer’s protocol. For the Dynabeads-based method, all the antibodies on the bead surface are accessible for optimal, highly reproducible antigen binding.</p></div><div class="heading section"><div class="h2"> Dynabeads magnetic beads compared to competitor magnetic beads </div></div><div class="image parbase section"><div id="cq-image-jsp-2b42f833-67f6-4f02-bda4-fa8bb0d55c1eMainParsysaccordion_9be8itemsparaccordionitem_1404itemParsysimage_6cb5"><a class="image-enlarge-link" data-lightbox="image_6cb5" href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/_jcr_content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_6cb5/backgroundimg.img.jpg/1725553120138.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_6cb5/foregroundimg.img.320.low.jpg/1725553120116.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_6cb5/foregroundimg.img.320.medium.jpg/1725553120116.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_6cb5/foregroundimg.img.480.medium.jpg/1725553120116.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_6cb5/foregroundimg.img.620.high.jpg/1725553120116.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_6cb5/foregroundimg.img.full.high.jpg/1725553120116.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_6cb5/foregroundimg.img.320.high.jpg/1725553120116.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_6cb5/foregroundimg.img.480.high.jpg/1725553120116.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_6cb5/foregroundimg.img.320.medium.jpg/1725553120116.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_6cb5/foregroundimg.img.620.high.jpg/1725553120116.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_6cb5/foregroundimg.img.320.low.jpg/1725553120116.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_6cb5/foregroundimg.img.480.medium.jpg/1725553120116.jpg" alt='Electron microscopy panel images of A) Dynabeads magnetic beads and B-D) magnetic beads from alternative suppliers ' title='Dynabeads magnetic beads compared to competitor magnetic beads'></picture><style> #cq-image-jsp-2b42f833-67f6-4f02-bda4-fa8bb0d55c1eMainParsysaccordion_9be8itemsparaccordionitem_1404itemParsysimage_6cb5 img{ width:100%; max-width: 1000px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"2b42f833-67f6-4f02-bda4-fa8bb0d55c1eMainParsysaccordion_9be8itemsparaccordionitem_1404itemParsysimage_6cb5 .image-contain" + "," + "#cq-image-jsp-"+"2b42f833-67f6-4f02-bda4-fa8bb0d55c1eMainParsysaccordion_9be8itemsparaccordionitem_1404itemParsysimage_6cb5 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div><div class="text parbase section"><p><b>Figure 4. Electron microscopy of Dynabeads magnetic beads compared to competitor magnetic beads.</b> (<b>A</b>) Dynabeads magnetic beads have a defined surface to carry out the necessary binding with no inner surface to trap any unwanted proteins. Dynabeads products are the most uniform, monodisperse superparamagnetic beads, manufactured with highly controlled product qualities to enable a high degree of reproducibility. (<b>B–D</b>) Magnetic particles from alternative suppliers have variable shapes and sizes that trap impurities, resulting in lower reproducibility and increased non-specific binding.</p></div><div class="heading section"><div class="h2"><br>Dynabeads magnetic beads compared to competitor magnetic beads for IP </div></div><div class="image parbase section"><div id="cq-image-jsp-2b42f833-67f6-4f02-bda4-fa8bb0d55c1eMainParsysaccordion_9be8itemsparaccordionitem_1404itemParsysimage_c675"><a class="image-enlarge-link" data-lightbox="image_c675" href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/_jcr_content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_c675/backgroundimg.img.jpg/1725553120187.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_c675/foregroundimg.img.320.low.jpg/1725553120165.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_c675/foregroundimg.img.320.medium.jpg/1725553120165.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_c675/foregroundimg.img.480.medium.jpg/1725553120165.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_c675/foregroundimg.img.620.high.jpg/1725553120165.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_c675/foregroundimg.img.full.high.jpg/1725553120165.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_c675/foregroundimg.img.full.high.jpg/1725553120165.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_c675/foregroundimg.img.320.high.jpg/1725553120165.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_c675/foregroundimg.img.480.high.jpg/1725553120165.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_c675/foregroundimg.img.320.medium.jpg/1725553120165.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_c675/foregroundimg.img.620.high.jpg/1725553120165.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_c675/foregroundimg.img.320.low.jpg/1725553120165.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_9be8/itemspar/accordionitem_1404/itemParsys/image_c675/foregroundimg.img.480.medium.jpg/1725553120165.jpg" alt='Silverstain blot (left) and western blot (right) showing Dynabeads Protein G magnetic beads have little non-specific binding and best signal/noise and target protein is most enriched using Dynabeads Protein G magnetic beads' title='Dynabeads magnetic beads compared to competitor magnetic beads for IP'></picture><style> #cq-image-jsp-2b42f833-67f6-4f02-bda4-fa8bb0d55c1eMainParsysaccordion_9be8itemsparaccordionitem_1404itemParsysimage_c675 img{ width:100%; max-width: 1580px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"2b42f833-67f6-4f02-bda4-fa8bb0d55c1eMainParsysaccordion_9be8itemsparaccordionitem_1404itemParsysimage_c675 .image-contain" + "," + "#cq-image-jsp-"+"2b42f833-67f6-4f02-bda4-fa8bb0d55c1eMainParsysaccordion_9be8itemsparaccordionitem_1404itemParsysimage_c675 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div><div class="text parbase section"><p><b>Figure 5. Dynabeads magnetic beads have exceptional immunoprecipitation performance with the combination of highest yield and lowest non-specific binding.</b> (<b>A</b>) Silver staining of the protein membrane shows that Dynabeads magnetic beads have the most enriched binding at the antibody main band and very low non-specific binding with low signal/noise. (<b>B</b>) Western blot analysis of CD81 pulldown in Jurkat cells show that Dynabeads Protein G beads have the highest yield with a short ten-minute protein incubation.</p><p><a class="cta-link" href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/immunoprecipitation-myths.html">Read tips on immunoprecipitation</a></p></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#4bf230458f674938b70c9b3d9345d1bf').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#4bf230458f674938b70c9b3d9345d1bf').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div class="text parbase section"><p><a class="cta-link" href="?open=antibody-ip#antibody-ip">View antibody-binding bead options for IP</a></p></div><div class="divider section"><hr /></div><div class="heading section"><h3 id="surface-beads"> Fusion tag-binding magnetic beads for IP </h3></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-1116988897"><tbody><tr><th>Tag</th><th>c-Myc</th><th>FLAG</th><th>HA</th><th>Histidine</th><th>GST</th></tr><tr><td><b>Description</b></td><td><ul><li>Consists of a peptide (EQKLISEEDL) derived from the human c-myc oncogene (p62 c-myc)</li><li>Immobilized anti-c-Myc antibodies are used to immunoprecipitate c-Myc-tagged recombinant proteins</li></ul></td><td><ul><li>Consists of a peptide (DYKDDDDK) which is recognized by an immobilized high-affinity rat monoclonal antibody (clone L5)</li><li>Primarily used for the isolation of protein complexes with multiple subunits because the mild purification process tends not to disrupt these interactions</li></ul></td><td><ul><li>Consists of a peptide (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein</li><li>Immobilized anti-HA antibodies are used to immunoprecipitate HA-tagged recombinant proteins</li></ul></td><td><ul><li>Consists of a string of six to nine histidine residues</li><li>Primarily used for purification via immobilized metal affinity chromatography (IMAC)</li></ul></td><td><ul><li>Consists of glutathione S-transferase (GST), a complete 211 amino acid protein (26 kDa)</li><li>Primarily used for purification via glutathione agarose resin</li></ul></td></tr><tr><td width="120" valign="bottom"><p style="text-align: left;"><b>Recommended products</b></p></td><td style="text-align: left;"><ul><li style="text-align: left;"><a href="/order/catalog/product/88842">Pierce Anti-c-Myc Magnetic Beads</a> </li><li style="text-align: left;"><a href="/order/catalog/product/88844">Pierce c-Myc-Tag Magnetic IP/Co-IP Kit </a></li></ul></td><td><ul><li><a href="/order/catalog/product/A36797">Pierce Anti-DYKDDDDK Magnetic Agarose</a></li></ul></td><td><ul><li><a href="/order/catalog/product/88836">Pierce Anti-HA Magnetic Beads</a> </li><li><a href="/order/catalog/product/88838">Pierce HA-Tag Magnetic IP/Co-IP Kit </a></li></ul></td><td><ul><li><a href="/order/catalog/product/10103D">Dynabeads His-Tag Isolation and Pulldown </a></li><li><a href="/order/catalog/product/78605">Pierce Ni-NTA Magnetic Agarose Beads </a></li><li><a href="/order/catalog/product/88831">HisPur Ni-NTA Magnetic Beads</a></li></ul></td><td><ul><li><a href="/order/catalog/product/78601">Pierce Glutathione Magnetic Agarose Beads</a></li></ul></td></tr></tbody></table></div><p>His and GST are not true epitope tags, because they are not usually purified or detected via specific antibodies. By contrast, HA and c-Myc tags are true epitope tags, because their only means of purification or detection is via specific antibodies. Epitope tags are seldom used for bulk purification but are most often used for IP or co-IP. Nevertheless, all four of these tag systems can be used for either purification or pull-down applications.</p><p><a class="cta-link" href="?open=fusion-ip#fusion-ip">View fusion tag-binding bead options for IP</a></p></div><div class="divider section"><hr /></div><div class="heading section"><h3 id="strep-beads"> Biotin-binding magnetic beads for IP </h3></div><div class="text parbase section"><p><a class="cta-link" href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads.html">If you have a biotinylated antibody (or ligand) that recognizes your protein, use of streptavidin beads is recommended</a></p><p>Main advantages of magnetic beads for biotin-binding:</p><ul><li>If you have a sample rich in soluble IgGs</li><li>If you have a recombinant antibody lacking Fc regions</li><li>If you need a bead compatible with mass spectrometry</li></ul><p><a class="cta-link" href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads.html?open=capture#capture">View streptavidin bead options for IP</a></p></div><div class="divider section"><hr /></div><div class="heading section"><h3 id="surf-beads"> Surface-activated magnetic beads for IP </h3></div><div class="text parbase section"><p><a class="cta-link" href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/surface-activated-dynabeads-magnetic-beads.html">If your target protein or ligand needs to be covalently coupled, use surface-activated beads</a></p><p>Different ligands (antibodies, proteins, etc.) require different bead surface properties and immobilization chemistries. Choose surface-activated beads to control factors such as:</p><ul><li>Hydrophobicity: choose hydrophilic of hydrophobic beads</li><li>Bead size: 1 µm, 2.8 µm, and 4.5 µm beads available</li><li>Surface chemistry: epoxy, amine, or tosylactivated</li></ul><p><a class="cta-link" href="?open=surf-ip#surf-ip">View surface-activated bead options for IP</a></p></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="well-cmp"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><div class="h2" id="automate-sample-preparation"> Automate your sample preparation using Dynabeads magnetic beads with protocols for Kingfisher instruments </div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div container-id="cq-colctrl-lt7" comp-id="A1"><div class="text parbase section"><p>KingFisher instruments automate the isolation of DNA, RNA, proteins, exosomes, viruses, and cells. KingFisher instruments help eliminate cross contamination, produce reproducible results, and cut your hands-on time down to 40 minutes. Set up plates, press start, and walk away.</p><p><a class="cta-link" href="/sg/en/home/life-science/dna-rna-purification-analysis/automated-purification-extraction/kingfisher-systems.html">Learn more about KingFisher instruments for automated protocols</a></p></div></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div container-id="cq-colctrl-lt7" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-fd598175-dace-4391-9fef-15325671dabcMainParsyswell_92dcuiparimage_16f3"><a href="/sg/en/home/life-science/dna-rna-purification-analysis/automated-purification-extraction/kingfisher-systems.html"><picture><source srcset='https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_92dc/uipar/image_16f3/foregroundimg.img.full.high.jpg/1710339445067.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_92dc/uipar/image_16f3/foregroundimg.img.full.high.jpg/1710339445067.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_92dc/uipar/image_16f3/foregroundimg.img.320.high.jpg/1710339445067.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_92dc/uipar/image_16f3/foregroundimg.img.480.high.jpg/1710339445067.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_92dc/uipar/image_16f3/foregroundimg.img.320.medium.jpg/1710339445067.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_92dc/uipar/image_16f3/foregroundimg.img.620.high.jpg/1710339445067.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_92dc/uipar/image_16f3/foregroundimg.img.320.low.jpg/1710339445067.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_92dc/uipar/image_16f3/foregroundimg.img.480.medium.jpg/1710339445067.jpg" alt='Three KingFisher automation family of instruments: Duo Prime, Flex, and Presto' title='Automated protocols with KingFisher instruments'></picture><style> #cq-image-jsp-fd598175-dace-4391-9fef-15325671dabcMainParsyswell_92dcuiparimage_16f3 img{ width:100%; max-width: 234px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"fd598175-dace-4391-9fef-15325671dabcMainParsyswell_92dcuiparimage_16f3 .image-contain" + "," + "#cq-image-jsp-"+"fd598175-dace-4391-9fef-15325671dabcMainParsyswell_92dcuiparimage_16f3 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="well-cmp"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><div class="h2"> Searching for high-performing, sustainable magnetic beads? Introducing DynaGreen Magnetic Beads. </div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-fd598175-dace-4391-9fef-15325671dabcMainParsyswell_3a02uiparimage_7ff0"><a href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/dynagreen-magnetic-beads.html"><picture><source srcset='https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_3a02/uipar/image_7ff0/foregroundimg.img.320.low.jpg/1710339444828.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_3a02/uipar/image_7ff0/foregroundimg.img.320.medium.jpg/1710339444828.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_3a02/uipar/image_7ff0/foregroundimg.img.480.medium.jpg/1710339444828.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_3a02/uipar/image_7ff0/foregroundimg.img.620.high.jpg/1710339444828.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_3a02/uipar/image_7ff0/foregroundimg.img.full.high.jpg/1710339444828.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_3a02/uipar/image_7ff0/foregroundimg.img.320.high.jpg/1710339444828.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_3a02/uipar/image_7ff0/foregroundimg.img.480.high.jpg/1710339444828.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_3a02/uipar/image_7ff0/foregroundimg.img.320.medium.jpg/1710339444828.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_3a02/uipar/image_7ff0/foregroundimg.img.620.high.jpg/1710339444828.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_3a02/uipar/image_7ff0/foregroundimg.img.320.low.jpg/1710339444828.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_3a02/uipar/image_7ff0/foregroundimg.img.480.medium.jpg/1710339444828.jpg" alt='Smart graph of sustainability factors of DynaGreen magnetic beads, which includes non-microplastic bead core, energy-efficient manufacturing, reduced water consumption, use of non-hazardous chemicals, recyclable packaging, and paperless documents' title='Sustainability factors of DynaGreen magnetic beads'></picture><style> #cq-image-jsp-fd598175-dace-4391-9fef-15325671dabcMainParsyswell_3a02uiparimage_7ff0 img{ width:100%; max-width: 906px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"fd598175-dace-4391-9fef-15325671dabcMainParsyswell_3a02uiparimage_7ff0 .image-contain" + "," + "#cq-image-jsp-"+"fd598175-dace-4391-9fef-15325671dabcMainParsyswell_3a02uiparimage_7ff0 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><p>DynaGreen Magnetic Beads provide high-quality, reproducible results and are also environmentally friendly and microplastic-free. How do these beads contribute to a more sustainable science? All DynaGreen Magnetic Beads have the following features:</p><ul><li>Non-microplastic bead core</li><li>Energy-efficient manufacturing</li><li>Reduced water consumption</li><li>Use of non-hazardous chemicals</li><li>Recyclable packaging</li><li>Paperless documents</li></ul><p><a class="cta-link" href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/dynagreen-magnetic-beads.html">Learn more about DynaGreen Magnetic Beads</a></p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div><div class="heading section"><h2 id="videos"><br>Videos on immunoprecipitation </h2></div><div class="brightcoveplaylist section"><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "Covalent Coupling of Antibodies to Magnetic Beads - Fast and Efficient", "description": "The benefits of the Dynabeads Antibody CoupliKit include: • Use any primary antibody • Get ultra-low non-specific binding • No need for cross-linking since Antibody is irreversibly bound to the beads and will not be eluted off with target protein", "thumbnailUrl": ["https://cf-images.us-east-1.prod.boltdns.net/v1/static/3663210762001/d94840f2-bf4a-4708-b28d-67bff29484ed/e2b12687-2526-4df0-9f82-1aff89c1a02c/160x82/match/image.jpg"], "uploadDate": "2017-11-18T01:06:46.206Z", "duration": "PT2M15.957S", "contentUrl": "https://edge.api.brightcove.com/playback/v1/accounts/3663210762001/videos/5650721847001/high.mp4?bcov_auth=eyJhbGciOiAiUlMyNTYiLCJ0eXAiOiAiSldUIn0.ewogICJpc3MiOiAiaHR0cHM6Ly93d3cudGhlcm1vZmlzaGVyLmNvbS8iLAogICJzdWIiOiAiY29udGVudF91cmwiLAogICJhdWQiOiAiaHR0cHM6Ly9icmlnaHRjb3ZlLmNvbSIKfQ.YqHusDPupPtMxztEAJqXZdheYqO3snN6xjms4Ui3zIPoj2yxzflQiCo4F8WHxA9NYzu3QGhPfCS_m8RFrLoa656z7RNfPGWsxJRa-IPjFvLAqhwJiJNWKBMoClWyGj0-groti1dghXWuGXxNraTU5GZ92tReZd9H8-g1ugo2hP_CV_qPtN8_uk6049UR1uXSXkiVf-bHrShqG1GDrTELzsHkLy3QAbyFkSlITfeaKcqGQO0nmAMPxOPbqHF5WjQwJEZyRmHOOGLXcy1PMRSUvtT9LUpJ6NOz8wgw6dfLpqdxnZOuSxwxTC6WRcnRSiokgpYuP6zYCopaoWUkeIzX3Q", "embedUrl": "//players.brightcove.net/3663210762001/hmATFP49Og_default/index.html?videoId=5650721847001", "@id": "//players.brightcove.net/3663210762001/hmATFP49Og_default/index.html?videoId=5650721847001&_="+new Date().getTime(), }; 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var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); </script><div class="video-container video-playlist-right"><div id="span-video-24b5cb856496" class="video-player-wrapper"><div style="margin-bottom: 0; margin-left: auto; margin-right: auto; margin-top: 0; overflow-x: hidden; overflow-y: hidden; text-align: center; width: 100%; text-align: center;"><div id="container-24b5cb856496" class="brightcove-container"><script>document.createElement('video')</script><video id="video-24b5cb856496" data-account="3663210762001" data-player="hmATFP49Og" data-embed="default" data-playlist-id="1779311462232013346" class="video-js" controls ></video><script src="//players.brightcove.net/3663210762001/hmATFP49Og_default/index.min.js"></script><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); 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Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplaylist, .cmp-p-videoplaylist")) } }, 'video-24b5cb856496',10); }); </script></div><div class="heading section"><h2 id="faqs"><br>Immunoprecipitation with Dynabeads magnetic beads FAQs </h2></div><div class="text parbase section"><p>Here are some frequently asked questions regarding the use of Dynabeads magnetic beads for immunoprecipitation. </p></div><div class="accordion section"><div class="accordion" id="0a689e77f71e4363b0e445f96b308d13" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#0a689e77f71e4363b0e445f96b308d13" href="#2"><span class="caret"></span> What is the difference between Dynabeads Protein A and Dynabeads Protein G? </a></div><div class="accordion-body collapse " id="2"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p><b>A: </b>Dynabeads Protein A and Dynabeads Protein G are 2.8-micron beads covalently coupled with Protein A and recombinant Protein G, respectively. Protein A and Protein G differ in their binding strength to immunoglobulins from different species and subclasses.</p><p><a class="cta-link" href="/sg/en/home/brands/product-brand/dynal/dynabeads-magnetic-separation-products-selection-guide.html">View the Dynabeads product selection guide for detailed information on Ig species and subclass specificity to Dynabeads</a></p></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#0a689e77f71e4363b0e445f96b308d13" href="#3"><span class="caret"></span> What can I do to improve binding of my antibody to Dynabeads Protein A or Protein G? </a></div><div class="accordion-body collapse " id="3"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p><b>A: </b>1. Verify binding/specificity of your antibody to your antigen (e.g., by ELISA).</p><p>2. Check the binding of your antibodies to the beads. If the antibodies are not captured and bound to the beads, the immunoprecipitation experiment will not work.</p><p>3. If you have used the indirect method of immunoprecipitation, try the direct method. Conversely, if you have used the direct method, try the indirect method.</p><p>4. Check the amount of beads and sample volume. With reference to the capacity of different beads proposed in the package inserts, increase the amount of beads or the concentration of your antibody during coupling.</p><p>5. Increase the incubation time.</p><p>6. Try another antibody.</p></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#0a689e77f71e4363b0e445f96b308d13" href="#4"><span class="caret"></span> Can I use larger (4.5 micron) magnetic beads for immunoprecipitation? </a></div><div class="accordion-body collapse " id="4"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p><b>A: </b>Yes, you can use the larger beads. <b>Note</b>: Smaller beads provide a larger surface area and therefore give higher yields of protein compared to the larger 4.5-micron beads.</p></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#0a689e77f71e4363b0e445f96b308d13').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#0a689e77f71e4363b0e445f96b308d13').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div class="text parbase section"><p><a class="cta-link" href="/search/results?query=immunoprecipitation%20magnetic%20beads&persona=DocSupport&refinementAction=true&filter=document.result_type_s:Product%20FAQs">View more FAQs about immunoprecipitation with magnetic beads</a></p></div><div class="accordion section"><div class="accordion" id="bc7feeada68142d290585eed14aba458" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#bc7feeada68142d290585eed14aba458" href="#publications"><span class="caret"></span> Publications on immunoprecipitation using Dynabeads magnetic beads </a></div><div class="accordion-body collapse " id="publications"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="heading section"><h2 id="publications"> Immunoprecipitation publications using Dynabeads magnetic beads </h2></div><div class="text parbase section"><ul><li><a href="https://pubmed.ncbi.nlm.nih.gov/30771180/" target="_blank">Brinkmalm A., Öhrfelt A., Bhattacharjee P., et al. (2019) Detection of α-Synuclein in Biological Samples Using Mass Spectrometry. Methods Mol Biol 1948:209–220.</a> <span class="icon icon-outbound"></span></li><li><a href="https://pubmed.ncbi.nlm.nih.gov/28601681/" target="_blank">Bafunno V., Firinu D., D’Apolito M., et al. (2018) Mutation of the angiopoietin-1 gene (ANGPT1) associates with a new type of hereditary angioedema. J Allergy Clin Immunol 141(3):1009–1017.</a> <span class="icon icon-outbound"></span></li><li><a href="https://pubmed.ncbi.nlm.nih.gov/28253434/" target="_blank">Stanton BZ., Hodges C., Crabtree GR., et al. (2017) A General Non-Radioactive ATPase Assay for Chromatin Remodeling Complexes. Curr Protoc Chem Biol 9(1):1–10. </a><span class="icon icon-outbound"></span></li></ul><p><a class="cta-link" href="/sg/en/home/life-science/cell-analysis/cell-isolation-and-expansion/cell-isolation.html?open=publications#publications">View publications on cell isolation using Dynabeads products</a><br><a class="cta-link" href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads.html?open=publications#publications">View publications on Dynabeads Streptavidin magnetic beads</a></p><p>Additional publications can be found in our <a href="/search/results?docTypes=Citations&persona=DocSupport&linkIn=true&query=&newSearch=true">Citations and References database</a>. The database can be searched using a product catalog number or keyword. For example, searching with the term "10001D" will return a list of journal articles that cite the use of Dynabeads Protein A for Immunoprecipitation (Cat. No. 10001D) and searching with the term "dynabeads" will return a list of journal articles that cite the use of any Dynabeads product. Note that the Citations and References database on thermofisher.com contains only the small subset of scientific journal articles that cite the use of our products. Many more can be found via targeted searches of the PubMed™ database and similar databases.</p></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#bc7feeada68142d290585eed14aba458').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#bc7feeada68142d290585eed14aba458').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div class="heading section"><div class="h2" id="order"><br>Ordering information </div></div><div class="accordion section"><div class="accordion" id="d3d893f4f3904b8aae54efeacf45d4f0" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#d3d893f4f3904b8aae54efeacf45d4f0" href="#antibodyip"><span class="caret"></span> Antibody-binding IP products </a></div><div class="accordion-body collapse " id="antibodyip"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">10001D,10002D,10008D,10003D,10004D,10009D,78609,78610,88802,88803,10006D,10007D,88804,88805,90409,11201D,11202D,11203D,11204D</div><div id='ltajax-6629574498531231594'></div><script type="text/javascript">(function() {$('#ltajax-6629574498531231594').load('/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_433f/itemspar/accordionitem_312/itemParsys/productlist_21e.ajax.html?skulist=');})(); </script></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#d3d893f4f3904b8aae54efeacf45d4f0" href="#fusionip"><span class="caret"></span> Recombinant protein (fusion tag) IP products </a></div><div class="accordion-body collapse " id="fusionip"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">10103D,10104D,78605,78606,88831,88832,78601,78602,88836,88837,88838,88842,88844,A36797,A36798</div><div id='ltajax-5579384343573028405'></div><script type="text/javascript">(function() {$('#ltajax-5579384343573028405').load('/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_433f/itemspar/accordionitem_1d71/itemParsys/productlist_2151.ajax.html?skulist=');})(); </script></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#d3d893f4f3904b8aae54efeacf45d4f0" href="#surfip"><span class="caret"></span> Surface-activated IP products </a></div><div class="accordion-body collapse " id="surfip"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">14301,14302D,14011,14311D,14321D,88826,88827,88828,65501,65502,14203,14204,14013</div><div id='ltajax-7749386059464070641'></div><script type="text/javascript">(function() {$('#ltajax-7749386059464070641').load('/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/jcr:content/MainParsys/accordion_433f/itemspar/accordionitem_6b45/itemParsys/productlist_724b.ajax.html?skulist=');})(); </script></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#d3d893f4f3904b8aae54efeacf45d4f0').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#d3d893f4f3904b8aae54efeacf45d4f0').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div class="well-cmp section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h2 id="resources"> Immunoprecipitation related content </h2></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><ul class="the-icons clearfix"><li><a href="/sg/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/immunoprecipitation-ip.html">Overview of the Immunoprecipitation (IP) Technique</a> </li><li><a href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/immunoprecipitation-myths.html">Myths of Immunoprecipitation</a> </li><li><a href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/dynabeads-immunoprecipitation-crosslinking-protocol.html">Crosslinking IP Protocol using Dynabeads</a> </li><li><a href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/automated-immunoprecipitation-protocols.html">Automation of IP on the KingFisher platform</a> </li><li><a href="/sg/en/home/life-science/protein-biology/protein-biology-learning-center.html">Protein Biology Learning Center</a> </li><li><a href="/sg/en/home/life-science/protein-biology/protein-biology-learning-center/protein-isolation-purification-information.html">Protein Isolation and Purification Information</a></li><li><a href="/sg/en/home/life-science/protein-biology/protein-biology-learning-center/protein-assays-analysis-information.html">Protein Assays and Analysis Information</a> </li><li><a href="/sg/en/home/technical-resources/technical-reference-library/protein-purification-isolation-support-center.html">Protein Purification and Isolation Support Center</a> </li><li><a href="/sg/en/home/technical-resources/technical-reference-library/protein-purification-isolation-support-center/protein-ip-coip-pulldown-support.html">Protein IP, Co-IP, and Pulldown Support</a> </li><li><a href="/sg/en/home/brands/product-brand/dynal/dynabeads-magnetic-separation-products-selection-guide.html">Selection Guide for IP</a></li><li><a href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads.html">Streptavidin-Coupled Dynabeads</a> </li><li><a href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/surface-activated-dynabeads-magnetic-beads.html">Surface-Activated Dynabeads</a> </li><li><a href="/sg/en/home/brands/product-brand/dynal/magnets.html">Magnetic Racks and Accessories</a></li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="heading section"><h3> Literature </h3></div></div><div container-id="cq-colctrl-lt0" comp-id="B2"><div class="text parbase section"><ul class="the-icons clearfix"><li><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/LSG/brochures/reproducible-immunoprecipitation-brochure.pdf" target="_blank">Brochure: Perform reproducible immunoprecipitation in less than 40 minutes</a></li><li><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/choose-high-performing-magnetic-beads-immunoprecipitation-brochure.pdf" target="_blank">Brochure: DynaGreen magnetic beads</a></li><li><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/dynabeads-magnetic-beads-key-successful-immunoprecipitation-app-note.pdf" target="_blank">Application note: Dynabeads magnetic beads—the key to successful immunoprecipitation </a></li><li><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/automated-immunoprecipitation-integration-protein-analysis-app-note.pdf" target="_blank">Application note: Automated immunoprecipitation with seamless integration to protein analysis</a></li><li><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/high-performance-environmentally-sustainable-magnetic-beads-app-note.pdf" target="_blank">Application note: High-performance, environmentally sustainable magnetic beads for immunoprecipitation to facilitate proteomics</a></li></ul></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="well-cmp"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><div class="h2"> Resources </div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><ul class="the-icons clearfix"><li><a href="/sg/en/home/brands/product-brand/dynal.html">Dynabeads Magnetic Bead Separation Technology</a></li><li><a href="/sg/en/home/life-science/dna-rna-purification-analysis/learning-center.html">DNA & RNA Learning Center</a></li><li><a href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation.html">Immunoprecipitation with Magnetic Beads</a></li><li><a href="/sg/en/home/life-science/cell-analysis/cell-isolation-and-expansion/cell-isolation.html">Cell Isolation</a></li><li><a href="/sg/en/home/life-science/cell-analysis/cell-isolation-and-expansion/cell-expansion.html">T Cell Activation & Expansion</a></li><li><a href="/sg/en/home/life-science/dna-rna-purification-analysis.html">Nucleic Acid Isolation</a></li><li><a href="/sg/en/home/life-science/cell-analysis/exosomes.html">Exosome Isolation, Detection, and Analysis</a></li><li><a href="/sg/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads.html">Streptavidin-Coupled Dynabeads</a></li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><ul class="the-icons clearfix"><li><a href="/sg/en/home/products-and-services/promotions/dynabeads-oem-plus.html">IVD Assay Development</a></li><li><a href="/sg/en/home/clinical/diagnostic-development/molecular-diagnostic-test-development/bead-based-ivd-assays/iso-certification.html">ISO Certification</a></li><li><a href="/sg/en/home/clinical/diagnostic-development/molecular-diagnostic-test-development/bead-based-ivd-assays/customized-dynabeads-oem-supply.html">Customized Beads for OEM</a></li><li><a href="/sg/en/home/clinical/diagnostic-development/molecular-diagnostic-test-development/bead-based-ivd-assays/intellectual-property.html">Intellectual Property</a></li><li><a href="/sg/en/home/technical-resources/contact-us.html">Technical Support</a></li></ul><ul class="the-icons clearfix"><li><span class="icon-lt-pdf"></span> <a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/dynabeads-magnetic-beads-brochure.pdf" target="_blank">Brochure: Dynabeads magnetic beads</a></li><li><span class="icon-lt-pdf"></span> <a href="https://assets.thermofisher.com/TFS-Assets/LSG/brochures/OEM_Capabilities.pdf" target="_blank">Flyer: OEM Capabilities</a></li></ul></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="ltrawhtml_a06 LTRawHTML"><style> /* Hero Banner Dark Grandient */ .wellPageHeading .well-content { background-image: 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